                         SEQUENCE LISTING

<110>  Biofidelity Limited
 
<120>  DETECTION METHOD

<130>  P32073WO1

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded first oligonucleotide 1

<400>  1
cgctcgatgt atacgctcgg accactcgta cctcgaactg tcgttagtat ttttatatgt       60

agtttctgaa gtagatatgg cagcacataa tgac                                   94


<210>  2
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded oligonucleotide 2

<400>  2
agtacaaata tgtcattatg tgctgccata tctacttcag aaactacata taaaaatact       60

aactttaagg                                                              70


<210>  3
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded oligonucleotide 3

<400>  3
agtacaaata tctcattatg tgctgccata tctacttcag aaactacata taaaaatact       60

aactttaagg                                                              70


<210>  4
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded oligonucleotide 4

<400>  4
agtacaaata tgtcattatg agctgccata tctacttcag aaactacata taaaaatact       60

aactttaagg                                                              70


<210>  5
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded oligonucleotide 5

<400>  5
agtacaaata tgtcattatg tgctgccata actacttcag aaactacata taaaaatact       60

aactttaagg                                                              70


<210>  6
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 1 a single-stranded oligonucleotide 6

<400>  6
agtacaaata tgtcattatg tgctgccata tctacttcag taactacata taaaaatact       60

aactttaagg                                                              70


<210>  7
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 2 a single-stranded oligonucleotide 1

<220>
<221>  C at position 1 has a 5' phosphate group
<222>  1

<400>  7
cgctcgatgt atacgctcgg accactcgta cctcgaactg tcgttagtat ttttatatgt       60

agtttctgaa gtagatatgg cagcacataa tgac                                   94


<210>  8
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 2 a single-stranded oligonucleotide 2

<220>
<221>  A at position 1 has a 5' phosphate group 
<222>  1

<400>  8
atgttcgatg aggcacgata tagatgtacg ctttgacata cgctttgaca atacttgagc       60

agtcggcaga tataggatgt tgcaagctcc gtgagtccca caaaccaata acctcgtttt      120

ttatatgtag tt                                                          132


<210>  9
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 2 a single-stranded oligonucleotide 3

<220>
<221>  C at position 57 is a 3'dideoxycytosine 
<222>  57

<400>  9
tatcgtgcct catcgaacat aactacatat aaaaaacgag gttattggtt tgtggcc          57


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 3 a single-stranded oligonucleotide primer 1

<400>  10
tgctcaagta ttgtcaaagc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 3 a single-stranded oligonucleotide primer 2

<400>  11
cggcagatat aggatgttgc                                                   20


<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 4 a single-stranded oligonucleotide 1

<220>
<221>  T at position 26 is a deoxythymidine labelled with Atto 594 dye
<222>  26

<220>
<221>  T at position 27 is a deoxythymidine labelled with Atto 594 dye
<222>  27

<220>
<221>  T at position 30 is a deoxythymidine labelled with BHQ-2 quencher
<222>  30

<400>  12
atgacctcgt aagccagtgt cagagtttttttc cagccgt                              40


<210>  13
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 4 a single-stranded oligonucleotide 2

<220>
<221>  T at position 51 is an inverted 3'dT 
<222>  51

<400>  13
ttcacacggc tggaaaaaaa ctctgacact ggcttacgag gtcattagatt                  51


<210>  14
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 T790M

<220>
<221>  A at position 1 has a 5' phosphate group
<222>  1

<400>  14
atgttcgatg agctttgaca atacttgagc acggcagata taggatgttg cgaagggcat       60

gagctgcatg atgagctg                                                     78


<210>  15
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 C797S

<220>
<221>  A at position 1 has a 5' phosphate group
<222>  1

<400>  15
atgttcgatg agctttgaca atacttgaag ctcgcagata taggatgttg cgatagtcca       60

ggaggctgc                                                               69


<210>  16
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 L861Q

<220>
<221>  A at position 1 has a 5' phosphate group
<222>  1

<400>  16
atgttcgatg agctttgaca atacttgatc gatgcagata taggatgttg cgatccgcac       60

ccagctgttt ggc                                                          73


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 T790M splint oligo

<400>  17
tgtcaaagct catcgaacat gcccttcgca acatct                                 36


<210>  18
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 C797S splint oligo

<400>  18
tgtcaaagct catcgaacat tcctggacta tcgcat                                 36


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 L861Q splint oligo

<400>  19
agctcatcga acatctgggt gcggatcgca acaa                                   34


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 T790M primer

<400>  20
acatcctata tctgccgt                                                     18


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 C797S primer

<400>  21
catcgaacat tcctggacta                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 L861Q primer

<400>  22
tcatcgaaca tctgggtgcg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 5 universal reverse primer

<400>  23
atgttcgatg agctttgaca                                                   20


<210>  24
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 synthetic  oligonucleotide 1

<400>  24
ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc ggaagagaaa       60

gaataccatg cagaaggagg                                                   80


<210>  25
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 synthetic 'mutant' oligonucleotide 2

<400>  25
ccgcagcatg tcaagatcac agattttggg cgggccaaac tgctgggtgc ggaagagaaa       60

gaataccatg cagaaggagg                                                   80


<210>  26
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 assay probe oligonucleotide 3

<220>
<221>  A at position 1 has a 5' phosphate group
<222>  1

<400>  26
atgttcgatg agctttgaca atacttgatc gatgcagata taggatgttg cgacagtttg       60

gcccgcccaa a                                                            71


<210>  27
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 assay probe oligonucleotide 4

<220>
<221>  A at position 1 has a 5' phosphate group
<222>  1

<400>  27
atgttcgatg agctttgaca atacttgatc gatgcagata taggatgttg cgacagtttg       60

gccggcccaa a                                                            71


<210>  28
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 splint olignucleotide 5

<400>  28
tgtcaaagct catcgaacat gccaaactgt cgcaag                                 36


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 primer oligonucleotide 6

<400>  29
tcgcaacatc ctatatctgc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example 8 primer oligonucleotide 7

<400>  30
tgagctttga caatacttga                                                   20


<210>  31
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  A at position 1 has a 5' phosphate.
<222>  1

<220>
<221>  A, T, G and T (positions 1-4) are all linked by phosphorothioate bonds.
<222>  1       

<400>  31
atgttcgatg agctttgaca atacttgaag ctcgcagata taggatgttg cgatagtcca       60

ggaggctgc                                                               69


<210>  32
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence

<400>  32
tgtcaaagct catcgaacat cctggactat gtctcc                                 36


<210>  33
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of human EGFR gene

<400>  33
tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg cccttcggca       60

gcctcctgga ctatg                                                        75


<210>  34
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Part of human EGFR gene with C797S mutation

<400>  34
tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg cccttcggct       60

gcctcctgga ctatg                                                        75


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence

<400>  35
tcgcaacatc ctatatctgc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA Sequence

<400>  36
tgagctttga caatacttga                                                   20


<210>  37
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT oligonucleotide

<400>  37
catctgcctc acctccaccg tgcagctcat cacgcagctc atgcccttcg gctgcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  38
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M oligonucleotide

<400>  38
catctgcctc acctccaccg tgcagctcat catgcagctc atgcccttcg gctgcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  39
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 oligonucleotide

<400>  39
catctgcctc acctccaccg tgcagctcat cacgcagctc atgcccttcg gcagcctcct       60

ggactatgtc cgggaacaca aagacaatat                                        90


<210>  40
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M probe. 

<220>
<221>  5' phosphate. 
<222>  1

<220>
<221>  Phosphorothioate bonds between A, T, G and T at positions 1-4.
<222>  1

<400>  40
atgttcgatg agctttgaca atacttgagc acggcagata taggatgttg cgaagggcat       60

gagctgcatg atgagctg                                                     78


<210>  41
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 probe. 

<220>
<221>  5' phosphate. 
<222>  1

<220>
<221>  Phosphorothioate bonds between A, T, G and T at positions 1-4.
<222>  1

<400>  41
atgttcgatg agctttgaca atacttgaag ctcgcagata taggatgttg cgatagtcca       60

ggaggctgc                                                               69


<210>  42
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T790M splint oligonucleotide.

<400>  42
tgtcaaagct catcgaacat gcccttcgca acatct                                 36


<210>  43
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C797S_2389 splint oligonucleotide.

<400>  43
tgtcaaagct catcgaacat tcctggacta tcgcat                                 36


<210>  44
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5 primer. 

<220>
<221> 3' Qusar670 dye, Quencher between G at position 50 
       and A at position 51.
<222> 50

<400>  44
acgcctggtt accgagccag gttcgcacat gtaggctcgg taaccaggcg acatcctata       60

tctgccgtgc                                                              70


<210>  45
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TexasRed primer. 5' TexasRed dye. Quencher between G at position 
       38 and C at position 39.

<400>  45
acgcctggtt acaggttcgc acatgtagta accaggcgca acatcctata tctgcgag         58


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer.

<400>  46
atgttcgatg agctttgaca                                                   20


<210>  47
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT oligonucleotide.

<400>  47
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

ggctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  48
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6239 oligonucleotide.

<400>  48
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  49
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6252 oligonucleotide.

<400>  49
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

agctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  50
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6253 oligonucleotide.

<400>  50
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

tgctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  51
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6239 probe oligonucleotide. 5' phosphate. Phosphorothioate 
       bonds between A, T, G and T at positions 1-4.

<400>  51
atgttcgatg agctttgaca atacttgaca tgcgcagata taggatgttg cgaaacgcac       60

cggaggccag cactttg                                                      77


<210>  52
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6252 probe oligonucleotide. 5' phosphate. Phosphorothioate 
       bonds between A, T, G and T at positions 1-4.

<400>  52
atgttcgatg agctttgaca atacttgaca tgccgagtaa tgagagtttc gcaaacgcac       60

cggagctcag cactttg                                                      77


<210>  53
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G719X_6253 probe oligonucleotide. 5' phosphate. Phosphorothioate 
       bonds between A, T, G and T at positions 1-4.

<400>  53
atgttcgatg agctttgaca atacttgaca tgcgagcaat taggtagtgt cgtaacgcac       60

cggagcacag cactttg                                                      77


<210>  54
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splint oligonucleotide.

<400>  54
tgtcaaagct catcgaacat ccggtgcgtt cggcaa                                 36


<210>  55
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 1. 5' Cy5 dye, phosphorothioate bond between A at 
       position 1 and C at position 2.

<400>  55
actgaccagc tccatgacaa tcgctgtcgc catgatcgat cgcaacatcc tatatctgc        59


<210>  56
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 2. 5' TEX dye, phosphorothioate bond between A at 
       position 1 and C at position 2.

<400>  56
actgaccagc tccatgacaa tcgctgtcgc catgatcgat gcgaaactct cattactcg        59


<210>  57
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Dye primer 3. 5' TEX dye, phosphorothioate bond between T at 
       position 1 and A at position 2.

<400>  57
tacgaccgac tcactcctta cagcagtccg cagtatgcta cgacactacc taattgctc        59


<210>  58
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quencher primer 1. 3' Iowa Black RQ quencher.

<400>  58
tcgatcatgg cgacagcgat tgtcatggag ctggtcagt                              39


<210>  59
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Quencher primer 2. 3' Iowa Black FQ quencher.

<400>  59
agcatactgc ggactgctgt aaggagtgag tcggtcgta                              39


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer. Phosphorothioate bonds between T, G and A at 
       positions 1-3.

<400>  60
tgagctttga caatacttga                                                   20



<210>  61
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C at positions 51, 58, 62, 63, 65, 66, 71, 75, 77 are 5-methyldeoxycytidine

<400>  61
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  62
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

<400>  62
accttacctt atacaccgtg ccgaacgcac cggaggccag cactttgatc tttttgaatt       60

cagtttcctt                                                              70


<210>  63
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

<400>  63
cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg       60

gcctccggtg cgttcggcac ggtgtataag gtaaggtccc                            100


<210>  64
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' phosphate, a,t,g,t at positions 1-4 linked by phosphorothioate bonds

<400>  64
atgttcgatg agctttgaca atacttgaca tgcgcagata taggatgttg cgaaacgcac       60

cggaggccag cactttg                                                      77


<210>  65
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

<400>  65
tgtcaaagct catcgaacat ccggtgcgtt cggcaa                                 36


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  t, c, g at positions 1-3 linked by phosphorothioate bonds

<400>  66
tcgcaacatc ctatatctgc                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  t, g, a at positions 1-3 linked by phosphorothioate bonds

<400>  67
tgagctttga caatacttga                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

<400>  68
ctcagcgtac ccttgtcccc aggaagcata cgtgatggca tacgtgatgg ctggtgtggg 	60

ctccccatat gtctcccgcc ttctgggcat					30


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223> c at position 20 is 5-methyldeoxycytidine 

<400>  69
ctcagcgtac ccttgtcccc aggaagcata cgtgatggca tacgtgatgg ctggtgtggg	60

ctccccatat gtctcccgcc ttctgggcat					30


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223> 5' phosphate, a-t at position 1-2 linked by phophorothioate bonds, 3' C3 spacer 

<400>  70
atgttcgatg agctttgaca atacttgatc gatgcagata taggatgttg cgaccatcac	60

gtatgccatc acgtatgctt cctggggaca ttt					33


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  

<400>  71
tgtcaaagct cagctatctg acgtgattcg caacaa 				36


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  t, c, g at positions 1-3 linked by phosphorothioate bonds

<400>  72
tcgcaacatc ctatatctgc                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  t, g, a at positions 1-3 linked by phosphorothioate bonds

<400>  73
tgagctttga caatacttga                                                   20


<210>  74
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype oligo

<400>  74
ctgctgggca tctgcctcac ctccaccgtg cagctcatca cgcagctcat gcccttcggc 	60

tgcctcctgg actatgtccg g 						21


<210>  75
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wildtype oligo

<400>  75
ctgctgggca tctgcctcac ctccaccgtg cagctcatca tgcagctcat gcccttcggc 	60

tgcctcctgg actatgtccg g 						21


<210>  76
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'phosphate.Phosphorothioate bonds betweeen a,g,c,t at 
positions 1-4.

<400>  76
agctgcatct gagctttgac aatacttgag cacggcagat ataggatgtt gcgaagggca	60

tgagctgcat gatg								14


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Phosphorothioate bonds betweeen t,c and g at positions 1-3.

<400>  77
tcgcaacatc ctatatctgc							20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223> 

<400>  78
atgttgcgaa gggcatatgt							20

