                         SEQUENCE LISTING

<110>  Lassogen, Inc.
 
<120>  LASSO PEPTIDES FOR TREATMENT OF CANCER

<130>  14619-007-228

<140>  TBA
<141>  

<150>  US 62/957,762
<151>  2020-01-06

<150>  US 62/980,918
<151>  2020-02-24

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 1

<400>  1

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 2

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is 7-hydroxyl-trptophan

<400>  2

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 3

<400>  3

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


<210>  4
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 4

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is 7-hydroxyl-trptophan

<400>  4

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 5

<400>  5

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr 
1               5                   10                  15  


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 6

<400>  6

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


Trp 
    


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 7

<400>  7

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
1               5                   10                  15      


<210>  8
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 8

<400>  8

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Phe 
1               5                   10                  15      


<210>  9
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 9

<400>  9

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Tyr 
1               5                   10                  15      


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 10

<400>  10

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Asn 
1               5                   10                  15      


Tyr Tyr Trp 
            


<210>  11
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 11

<400>  11

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Asn 
1               5                   10                  15      


Ile Ile Trp 
            


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 12

<400>  12

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
1               5                   10                  15      


His Leu Asp Ile Ile Trp 
            20          


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 13

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa = (2S)-2-amino-3-(1H-pyrrolo[5,4-b]pyridin-3-yl)propanoic 
(Aza derivative of Tryptophan)

<400>  13

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  14
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 14

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Tryptophan having a C-terminal methyl ester group (-CO2Me)
 in place of the carboxylic acid group (-CO2H)

<400>  14

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 15

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Tryptophan having a C-terminal benzyl ester group 
(-CO2Bn) in place of the carboxylic acid group (-CO2H)

<400>  15

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 16

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Tryptophan having a C-terminal amide group 
(-CONH2) in place of the carboxylic acid group (-CO2H)

<400>  16

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  17
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 17

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is 2-naphthylalanine

<400>  17

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  18
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 1

<400>  18

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
        35                  40                  


<210>  19
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 2

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is 7-hydroxyl-trptophan

<400>  19

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  20
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 3

<400>  20

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
        35                  40                  


<210>  21
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 4

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is 7-hydroxyl-trptophan

<400>  21

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  22
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 5

<400>  22

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr 
        35                  40              


<210>  23
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 6

<400>  23

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp Trp 
        35                  40                  45  


<210>  24
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 7

<400>  24

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
        35                  40                  


<210>  25
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 8

<400>  25

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Phe 
        35                  40                  


<210>  26
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 9

<400>  26

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Tyr 
        35                  40                  


<210>  27
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 10

<400>  27

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Asn Tyr Tyr Trp 
        35                  40                  45          


<210>  28
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 11

<400>  28

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Asn Ile Ile Trp 
        35                  40                  45          


<210>  29
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 12

<400>  29

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Ala His Leu Asp Ile 
        35                  40                  45              


Ile Trp 
    50  


<210>  30
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 13

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa = (2S)-2-amino-3-(1H-pyrrolo[5,4-b]pyridin-3-yl)propanoic 
(Aza derivative of Tryptophan)

<400>  30

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  31
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 14

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is Tryptophan having a C-terminal methyl ester group (-CO2Me)
 in place of the carboxylic acid group (-CO2H)

<400>  31

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  32
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 15

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is Tryptophan having a C-terminal benzyl ester group 
(-CO2Bn) in place of the carboxylic acid group (-CO2H)

<400>  32

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  33
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 16

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Tryptophan having a C-terminal amide group (-CONH2) in place of the carboxylic acid group (-CO2H)

<400>  33

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  34
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 17

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is 2-naphthylalanine

<400>  34

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  35
<211>  145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lasso peptidase 

<400>  35

Met Ser Gln Ser Met Ala Leu Asp Gln Arg Ala Arg Leu Pro Leu Ala 
1               5                   10                  15      


Arg Arg Leu Leu Pro Leu Leu Ala Val Gly Ala Ala Arg Pro Leu Ala 
            20                  25                  30          


Arg Leu Lys Pro Ala Arg Leu Arg Ala Val Leu Glu Phe Ala Arg Arg 
        35                  40                  45              


Gly Ala Ala Pro Ala Gly Ala Gly Gln Ala Gln Arg Ala Arg Glu Gln 
    50                  55                  60                  


Val Val Ser Val Ser Leu Arg Cys Ala Gly Gln Asn Cys Leu Gln Arg 
65                  70                  75                  80  


Ser Leu Ala Thr Val Leu Leu Cys Arg Ala Arg Gly Val Trp Pro Thr 
                85                  90                  95      


Trp Cys Thr Gly Val Arg Thr His Pro Phe Ala Ala His Ala Trp Val 
            100                 105                 110         


Glu Ala Glu Gly Arg Leu Ile Gly Glu Pro His Pro Glu Gly Tyr Tyr 
        115                 120                 125             


Lys Pro Leu Leu Thr Val Pro Pro Arg Thr Gly Ala Gly Ala Gly Asp 
    130                 135                 140                 


Arg 
145 


<210>  36
<211>  615
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lasso cyclase 

<400>  36

Met Ala Lys Leu Ser Ala Gly Phe Val Val Leu Pro Asp Thr Ala Asp 
1               5                   10                  15      


Gly Ala Glu Ile Arg Ala Leu Val Pro Phe Ala Pro Ala Arg Val Ile 
            20                  25                  30          


Ala His Ala Ser Gly Arg Pro Trp Leu Val Gly Glu Trp Ser Gln Gly 
        35                  40                  45              


Gln Val Arg Val Ala Ser Ala Gly Pro Val Arg Leu Ala Val Ser Gly 
    50                  55                  60                  


Thr Cys Pro Val Thr Asp Asp Gly Leu Ala Arg Ile Ala Ala Arg Ile 
65                  70                  75                  80  


Ser Arg Leu Ser Glu Val Asp Arg Ala Val Pro Ala Leu Leu Gly Ser 
                85                  90                  95      


Cys His Ile Val Ala Ser Val Asp Gly His Val Arg Phe Gln Gly Ser 
            100                 105                 110         


Ala Ser Gly Leu Arg Arg Val Phe His Thr Arg Val Asn Gly Val Arg 
        115                 120                 125             


Val Ala Ala Asp Arg Ser Asp Val Leu Ala Ala Met Thr Gly Ala Gly 
    130                 135                 140                 


Val Asp Glu Glu Thr Leu Ala Leu His Val Ala Cys Gly Leu Gln Val 
145                 150                 155                 160 


Pro Phe Pro Ala Asn Ala Arg Ser Ala Trp Ser Gly Ile Ser Thr Leu 
                165                 170                 175     


Ala Pro Glu Asn Cys Leu Leu Trp Glu Gly Asp Arg Asp Arg Glu Thr 
            180                 185                 190         


Val Trp Trp Arg Pro Pro Glu Pro Asp Arg Ser Leu Arg Glu Gly Thr 
        195                 200                 205             


Ala Ala Val Arg Asp Thr Leu Ala Ala Val Val Gly Arg Gln Ala Pro 
    210                 215                 220                 


Val Glu Gly Arg Leu Ser Ala Asp Leu Ser Gly Gly Leu Asp Ser Thr 
225                 230                 235                 240 


Ser Leu Cys Phe Leu Ala Ala Arg His Thr Pro Glu Leu Leu Thr Phe 
                245                 250                 255     


Arg Trp Gly Glu Ala Asp Ala Gly Asn Asp Asp Ala Val Tyr Ala Gly 
            260                 265                 270         


Gln Ala Ala Arg Leu Leu Asp Arg Ala Glu His Leu Val Val Pro Gln 
        275                 280                 285             


His Glu Leu Pro Asp Ile Phe Ala Asp Pro Ala Leu Ala Val Ser Ala 
    290                 295                 300                 


Glu Glu Pro Leu Ser Leu Thr Arg Ala Thr Ala Arg Ile Arg Arg Gly 
305                 310                 315                 320 


Ala Arg Leu Leu Ala Asp Arg Gly Ser Arg Arg His Leu Ala Gly His 
                325                 330                 335     


Gly Gly Asp Glu Leu Phe Ser Pro Met Pro Ser Tyr Leu His Gln Leu 
            340                 345                 350         


Met Arg Arg His Pro Val Thr Ala Leu Lys His Val Arg Ala His Cys 
        355                 360                 365             


Ala Leu Lys Arg Trp Pro Leu Lys Ala Thr Leu Ala Ala Leu Ala Gln 
    370                 375                 380                 


Pro Gly Ser Leu Pro Ala Trp Trp Arg Glu Gln Ala Gly Leu Leu Thr 
385                 390                 395                 400 


Glu Pro Arg Pro Pro Leu Arg Arg Pro Ala Leu Gly Trp Gly Leu Gly 
                405                 410                 415     


Pro Val Arg Ala Ala Pro Trp Met Thr Pro Glu Gly Val Glu Leu Ala 
            420                 425                 430         


Arg Glu Ala Leu Arg Arg Thr Ala Glu Trp Ala Gln Pro Leu Ala Glu 
        435                 440                 445             


Asp Leu Ala Thr His Thr Met Val Leu Met Ile Arg Ser Asn Thr Ala 
    450                 455                 460                 


Ser Tyr Arg Leu Leu Ala Arg Leu Tyr Ala Glu Ala Gly Val Glu Leu 
465                 470                 475                 480 


Asp Met Pro Tyr Leu Asp Asp Ala Val Ile Asp Ala Val Leu Arg Val 
                485                 490                 495     


Pro Ala His Ala His Ala Gly Pro Trp Arg Tyr Lys Pro Leu Leu Ala 
            500                 505                 510         


Asp Ala Met His Gly Ile Val Pro Asp Gly Ile Arg Ala Arg Ser Thr 
        515                 520                 525             


Lys Gly Glu Phe Gly Glu Asp Ile Arg Arg Gly Leu Arg Arg Asn Leu 
    530                 535                 540                 


Pro Ala Ile Leu Asp Leu Phe Ala Asp Ser Glu Leu Ala Ala Arg Gly 
545                 550                 555                 560 


Leu Ile Asp Thr Gly Ala Leu Arg Leu Arg Leu Gly Gly Pro Gln Pro 
                565                 570                 575     


Asp Asn Thr Thr Ala Gln Ala Leu Glu Ser Leu Leu Gly Cys Glu Thr 
            580                 585                 590         


Trp Leu Arg Thr Thr Ser Gly Pro Gly Thr Ile Pro Arg Ala Gly Asp 
        595                 600                 605             


Gly Thr Val Pro Ser Glu Val 
    610                 615 


<210>  37
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lasso RRE 

<400>  37

Met Arg Leu His Pro Asp Val Ser Met Thr Asp Thr Asp Asp Gly Thr 
1               5                   10                  15      


Val Leu Leu His Gln Arg Thr Gly Arg Tyr Trp Gln Leu Asn Val Thr 
            20                  25                  30          


Gly Ser Arg Val Leu His Arg Leu Leu Asp Gly Asp Thr Pro Glu Thr 
        35                  40                  45              


Ile Ala Asp Gly Leu Ala Ala Ala His Gly Ile Asp Pro Gln Arg Ala 
    50                  55                  60                  


Arg Gln Asp Val Gly Ala Val Ile Glu Gln Leu Arg Thr Ala Glu Leu 
65                  70                  75                  80  


Thr Val Ala Ser 
                


<210>  38
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Precursor Peptide for Lasso 3

<400>  38
atgaccgagc agtccgagca gacacccgcg gaatacattc cgccgatgct cgtcgaggtc       60

ggcgagttca ccgaagacac gttgggcaac tggcacggca catcgcccga ctggttcttc      120

aactactact gg                                                          132


<210>  39
<211>  435
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lasso peptidase 

<400>  39
atgagccagt ccatggcact ggaccagcgg gcccggctcc cgcttgcgcg ccgtctgctg       60

ccgctgctgg ccgtcggcgc cgcccggccg ctcgcccgcc tcaagcccgc gcggctgcgc      120

gccgtgctcg aattcgcccg ccgtggtgcg gcgccggcgg gtgccgggca ggcgcagcgg      180

gcgcgcgagc aggtcgtctc ggtgagcctg cgctgtgcgg ggcagaactg tctgcagcgc      240

tcgctcgcca ccgtgctgct gtgccgggcc cgcggggtgt ggccgacctg gtgcaccggc      300

gtacggacgc acccgttcgc cgcccacgcc tgggttgagg ccgagggccg gctgatcggc      360

gaaccgcacc ccgagggcta ttacaagccg ctgctgaccg tcccgccccg caccggagcc      420

ggcgccgggg accgc                                                       435


<210>  40
<211>  1845
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lasso cyclase 

<400>  40
atggcgaagt tgagtgctgg gtttgtggtc ctgccggaca ccgccgacgg tgcggagatc       60

cgggcgctcg taccgttcgc gcctgcccga gtgatcgcgc acgcctccgg gcggccgtgg      120

ctggtcggcg agtggtctca gggtcaggtg agggtggctt cggcgggtcc ggtacgtctg      180

gccgtgagcg gcacgtgtcc ggtcaccgac gacgggctgg cgcggattgc cgcccgtatc      240

tcccggctgt ccgaggtgga ccgggccgtt cccgcgctgc tggggtcctg ccacatcgtg      300

gcatcggtgg acgggcacgt ccggttccag ggcagcgcct ccgggctgcg tcgcgtgttc      360

cacacccggg tgaacggggt gcgggtggcc gcggaccggt ccgatgtgct cgcggcgatg      420

accggtgccg gggtggacga ggagacgctg gcactgcacg tcgcatgcgg tttgcaggtg      480

ccctttccgg ccaacgcgcg cagcgcgtgg tccggcatca gcacgctcgc gccggagaac      540

tgtctgctgt gggagggcga ccgggaccgg gaaacggtgt ggtggcgtcc gcccgagccg      600

gaccggtcgt tgcgggaggg gacggcagcg gtacgggaca ccctggctgc cgtagtgggc      660

cgacaagcgc ccgtcgaagg ccggttgagt gcggatctgt ccggcgggct ggattcgacg      720

tcgctgtgtt ttctggcggc ccggcacaca ccggagctgc tgaccttccg gtggggcgag      780

gccgacgcgg gcaatgacga cgcggtctac gcggggcagg cggcgcggct gctcgaccgt      840

gccgagcacc tcgtcgtgcc gcagcacgaa ctccccgaca tcttcgccga cccggccctc      900

gcggtgtccg ccgaggagcc gctgtcgctg acgcgcgcga cggcccggat ccgccggggc      960

gcccgactgc tcgcggaccg cggctcccgt cggcatctgg ccgggcacgg cggcgacgag     1020

ctgttctccc cgatgccctc ctatctgcat cagttgatgc gccgtcatcc ggtgacggcg     1080

ctcaagcacg tacgggcgca ctgcgcgctg aagcgctggc cgctgaaggc gacgcttgcc     1140

gcgctcgcgc agccgggaag tctccccgcg tggtggcggg agcaggccgg gctgctgacc     1200

gagccccggc cgccgctgcg gcgcccggcg ctgggctggg gtctgggacc cgtacgtgcg     1260

gcgccctgga tgacccccga gggcgtcgag ctggcgcggg aggcgctgcg ccggaccgcc     1320

gagtgggcgc agccgctcgc cgaggacctc gccacccaca cgatggtgct catgatccgt     1380

tcgaacacgg ccagttaccg gttgctggcg cggctgtacg ccgaggccgg tgtggagctg     1440

gacatgccgt atctggacga cgccgtgatc gacgcggtgc tccgggtacc ggcgcacgcg     1500

cacgccggac cgtggcggta caagcccctg ctggccgatg cgatgcacgg catcgtgccg     1560

gacggcatcc gggcgcggtc caccaagggc gagttcggtg aggacatcag aagggggctg     1620

cgtcgcaacc tgcccgccat cctggacctc ttcgccgatt ccgagctggc ggcccgcggt     1680

ctgatcgaca ccggcgcgct ccggctgcgg ctgggcgggc cgcagccgga caacaccacc     1740

gcccaggcgc tggagagcct gctcggctgc gagacctggc tgcgcaccac gtccggcccc     1800

ggcaccatcc cccgtgccgg cgacggcacc gttccctcgg aggtc                     1845


<210>  41
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lasso RRE 

<400>  41
atgcggctcc atcccgacgt ctccatgacg gacaccgacg acggcacggt gctgctgcat       60

cagcgcaccg gccgctactg gcagctcaac gtcaccggca gccgcgtgct gcaccggctg      120

ctggacgggg acacccccga gaccatcgcc gacggcctcg ccgccgcgca cggcatcgac      180

ccgcagcggg cccggcagga tgtcggcgcc gtgatcgagc aactgcgcac cgccgagctg      240

acggtggcct ca                                                          252


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 42

<400>  42

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr 
1               5                   10                  15  


<210>  43
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 43

<400>  43

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


Trp 
    


<210>  44
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 44

<400>  44

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
1               5                   10                  15      


<210>  45
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 45

<400>  45

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Phe 
1               5                   10                  15      


<210>  46
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 46

<400>  46

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Tyr 
1               5                   10                  15      


<210>  47
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 47

<400>  47

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Asn 
1               5                   10                  15      


Tyr Tyr Trp 
            


<210>  48
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 48

<400>  48

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Asn 
1               5                   10                  15      


Ile Ile Trp 
            


<210>  49
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 49

<400>  49

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
1               5                   10                  15      


His Leu Asp Ile Ile Trp 
            20          


<210>  50
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 50

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa = (2S)-2-amino-3-(1H-pyrrolo[5,4-b]pyridin-3-yl)propanoic 
(Aza derivative of Tryptophan)

<400>  50

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  51
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 51

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Tryptophan having a C-terminal methyl ester group (-CO2Me)
 in place of the carboxylic acid group (-CO2H)

<400>  51

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  52
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 52

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is Tryptophan having a C-terminal benzyl ester group 
(-CO2Bn) in place of the carboxylic acid group (-CO2H)

<400>  52

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  53
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 53

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Tryptophan having a C-terminal amide group (-CONH2) in place of the carboxylic acid group (-CO2H)

<400>  53

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  54
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 54

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is 2-naphthylalanine

<400>  54

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
1               5                   10                  15      


<210>  55
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 55

<400>  55

Gly Asn Trp His Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


Ile Ile Trp 
            


<210>  56
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Core Peptide for Lasso 56

<400>  56

Gly Asn Trp His Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp 
1               5                   10                  15      


Ile Ile Trp 
            


<210>  57
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 42

<400>  57

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr 
        35                  40              


<210>  58
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 43

<400>  58

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp Trp 
        35                  40                  45  


<210>  59
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 44

<400>  59

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Ala 
        35                  40                  


<210>  60
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 45

<400>  60

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Phe 
        35                  40                  


<210>  61
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 46

<400>  61

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Tyr 
        35                  40                  


<210>  62
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 47

<400>  62

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Asn Tyr Tyr Trp 
        35                  40                  45          


<210>  63
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 48

<400>  63

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Asn Ile Ile Trp 
        35                  40                  45          


<210>  64
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 49

<400>  64

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Ala His Leu Asp Ile 
        35                  40                  45              


Ile Trp 
    50  


<210>  65
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 50

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa = (2S)-2-amino-3-(1H-pyrrolo[5,4-b]pyridin-3-yl)propanoic 
(Aza derivative of Tryptophan)

<400>  65

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  66
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 51

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is Tryptophan having a C-terminal methyl ester group (-CO2Me)
 in place of the carboxylic acid group (-CO2H)

<400>  66

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  67
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 52

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is Tryptophan having a C-terminal benzyl ester group 
(-CO2Bn) in place of the carboxylic acid group (-CO2H)

<400>  67

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  68
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 53

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Tryptophan having a C-terminal amide group (-CONH2) in place of the carboxylic acid group (-CO2H)

<400>  68

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  69
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 54

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  Xaa is 2-naphthylalanine

<400>  69

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Xaa 
        35                  40                  


<210>  70
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 55

<400>  70

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ala Pro Asp Trp Phe Phe Asn Tyr Tyr Trp Ile Ile Trp 
        35                  40                  45          


<210>  71
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Precursor peptide for Lasso 56

<400>  71

Met Thr Glu Gln Ser Glu Gln Thr Pro Ala Glu Tyr Ile Pro Pro Met 
1               5                   10                  15      


Leu Val Glu Val Gly Glu Phe Thr Glu Asp Thr Leu Gly Asn Trp His 
            20                  25                  30          


Gly Thr Ser Pro Asp Trp Phe Phe Asn Tyr Tyr Trp Ile Ile Trp 
        35                  40                  45          


<210>  72
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  72

Ser Glu Lys Asp Arg Glu Gly Val Arg Ile Arg Glu Arg Gly Ala Val 
1               5                   10                  15      


Arg Lys Ser Leu Pro Ala 
            20          


