                         SEQUENCE LISTING

<110>  NLC PHARMA LTD
       ARAD, Dorit
 
<120>  RAPID DETECTION TEST FOR SARS-COV-2

<130>  82013

<150>  US 62/972,005
<151>  2020-02-09

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  EDANS 3' conjugated

<400>  1

Lys Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys Met Glu 
1               5                   10                  


<210>  2
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  EDANS 3' conjugated

<400>  2

Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys 
1               5                   10      


<210>  3
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  EDANS 3' conjugated

<400>  3

Thr Ser Ala Val Leu Gln Ser Gly Phe 
1               5                   


<210>  4
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  EDANS 3' conjugated

<400>  4

Ala Val Leu Gln Ser Gly Phe 
1               5           


<210>  5
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  EDANS 3' conjugated

<400>  5

Ala Val Leu Gln Ser Gly Phe Arg Lys 
1               5                   


<210>  6
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  EDANS 3' conjugated

<400>  6

Thr Ser Ala Val Leu Gln Ser Gly Phe Tyr Lys 
1               5                   10      


<210>  7
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  BHQ-1 conjugated

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  AF488 conjugated

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  AMIDATION

<400>  7

Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys Cys 
1               5                   10          


<210>  8
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  BHQ-1 conjugated

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  AF488 conjugated

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  AMIDATION

<400>  8

Thr Ser Ala Val Leu Gln Ser Gly Phe Cys Arg Lys 
1               5                   10          


<210>  9
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  BHQ-1 conjugated

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  AF488 conjugated

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  AMIDATION

<400>  9

Ala Val Leu Gln Ser Gly Phe Cys Arg Lys 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  BHQ-1 conjugated

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  AF488 conjugated

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  AMIDATION

<400>  10

Ala Val Leu Gln Ser Gly Phe Arg Lys Cys 
1               5                   10  


<210>  11
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl  conjugated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  EDANS 3' conjugated

<400>  11

Tyr Gly Ser Thr Leu Gln Ala Gly Leu Arg Lys 
1               5                   10      


<210>  12
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Dabcyl 5' conjugated

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  EDANS  conjugated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  AMIDATION

<400>  12

Leu Glu Ala Leu Phe Gln Gly Pro Asp Ser Gln 
1               5                   10      


<210>  13
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  13

Ala Val Leu Gln Ser Gly Phe 
1               5           


<210>  14
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  14

Thr Phe Gln Ser Ala Val 
1               5       


<210>  15
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  15

Thr Val Gln Ser Lys Met 
1               5       


<210>  16
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  16

Thr Leu Gln Ala Ile Ala Ser 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  17

Lys Leu Gln Asn Asn Glu Leu 
1               5           


<210>  18
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  18

Arg Leu Gln Ala Gly Asn 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  19

Met Leu Gln Ser Ala Asp 
1               5       


<210>  20
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  20

Val Leu Gln Ala Val Gly 
1               5       


<210>  21
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  21

Thr Leu Gln Ala Glu Asn Val 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  22

Arg Leu Gln Ser Leu Glu Asn 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  23

Lys Leu Gln Ser Ser Gln Ala 
1               5           


<210>  24
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  24

Lys Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys Met Glu 
1               5                   10                  


<210>  25
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  25

Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys 
1               5                   10      


<210>  26
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  26

Thr Ser Ala Val Leu Gln Ser Gly Phe 
1               5                   


<210>  27
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  27

Ala Val Leu Gln Ser Gly Phe 
1               5           


<210>  28
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  28

Ala Val Leu Gln Ser Gly Phe Arg Lys 
1               5                   


<210>  29
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide

<400>  29

Thr Ser Ala Val Leu Gln Ser Gly Phe Tyr Lys 
1               5                   10      


<210>  30
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Xaa can be any naturally occurring amino acid

<400>  30

Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys Xaa 
1               5                   10          


<210>  31
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<400>  31

Thr Ser Ala Val Leu Gln Ser Gly Phe Xaa Arg Lys 
1               5                   10          


<210>  32
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Xaa can be any naturally occurring amino acid

<400>  32

Ala Val Leu Gln Ser Gly Phe Xaa Arg Lys 
1               5                   10  


<210>  33
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  synthetic peptide


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Ala Val Leu Gln Ser Gly Phe Arg Lys Xaa 
1               5                   10  


<210>  34
<211>  306
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3CL protease of the SARS-CoV-2 virus

<400>  34

Ser Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys 
1               5                   10                  15      


Met Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu 
            20                  25                  30          


Asp Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp 
        35                  40                  45              


Met Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His 
    50                  55                  60                  


Asn Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His 
65                  70                  75                  80  


Ser Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro 
                85                  90                  95      


Lys Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe 
            100                 105                 110         


Ser Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys 
        115                 120                 125             


Ala Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser 
    130                 135                 140                 


Cys Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys 
145                 150                 155                 160 


Tyr Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp 
                165                 170                 175     


Leu Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln 
            180                 185                 190         


Ala Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu 
        195                 200                 205             


Tyr Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr 
    210                 215                 220                 


Thr Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu 
225                 230                 235                 240 


Pro Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln 
                245                 250                 255     


Thr Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu 
            260                 265                 270         


Gln Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu 
        275                 280                 285             


Asp Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr 
    290                 295                 300                 


Phe Gln 
305     


<210>  35
<211>  320
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3CL protease of the SARS-CoV-2 virus with the histidine tag and 
       cleavable sequence

<400>  35

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Ser Gly 
1               5                   10                  15      


Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys Met Val 
            20                  25                  30          


Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu Asp Asp 
        35                  40                  45              


Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp Met Leu 
    50                  55                  60                  


Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His Asn Phe 
65                  70                  75                  80  


Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His Ser Met 
                85                  90                  95      


Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro Lys Thr 
            100                 105                 110         


Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe Ser Val 
        115                 120                 125             


Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys Ala Met 
    130                 135                 140                 


Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser Cys Gly 
145                 150                 155                 160 


Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys Tyr Met 
                165                 170                 175     


His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp Leu Glu 
            180                 185                 190         


Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln Ala Ala 
        195                 200                 205             


Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu Tyr Ala 
    210                 215                 220                 


Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr Thr 
225                 230                 235                 240 


Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro Leu 
                245                 250                 255     


Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr Gly 
            260                 265                 270         


Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu Gln Asn 
        275                 280                 285             


Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp Glu 
    290                 295                 300                 


Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr Phe Gln 
305                 310                 315                 320 


<210>  36
<211>  183
<212>  PRT
<213>  Artificial sequence

<220>
<223>  sequence of HRV 3C protease

<400>  36

Gly Pro Glu Glu Glu Phe Gly Met Ser Ile Ile Lys Asn Asn Thr Cys 
1               5                   10                  15      


Val Val Thr Thr Thr Asn Gly Lys Phe Thr Gly Leu Gly Ile Tyr Asp 
            20                  25                  30          


Arg Ile Leu Ile Leu Pro Thr His Ala Asp Pro Gly Ser Glu Ile Gln 
        35                  40                  45              


Val Asn Gly Ile His Thr Lys Val Leu Asp Ser Tyr Asp Leu Phe Asn 
    50                  55                  60                  


Lys Glu Gly Val Lys Leu Glu Ile Thr Val Leu Lys Leu Asp Arg Asn 
65                  70                  75                  80  


Glu Lys Phe Arg Asp Ile Arg Lys Tyr Ile Pro Glu Ser Glu Asp Asp 
                85                  90                  95      


Tyr Pro Glu Cys Asn Leu Ala Leu Val Ala Asn Gln Thr Glu Pro Thr 
            100                 105                 110         


Ile Ile Lys Val Gly Asp Val Val Ser Tyr Gly Asn Ile Leu Leu Ser 
        115                 120                 125             


Gly Thr Gln Thr Ala Arg Met Leu Lys Tyr Asn Tyr Pro Thr Lys Ser 
    130                 135                 140                 


Gly Tyr Cys Gly Gly Val Leu Tyr Lys Ile Gly Gln Ile Leu Gly Ile 
145                 150                 155                 160 


His Val Gly Gly Asn Gly Arg Asp Gly Phe Ser Ser Met Leu Leu Arg 
                165                 170                 175     


Ser Tyr Phe Thr Glu Gln Gln 
            180             


<210>  37
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  cleavable tag

<400>  37

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  38
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  38

Thr Ser Ala Val Leu Gln Ser Gly Phe Cys Arg Lys 
1               5                   10          


<210>  39
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  39

Thr Ser Ala Val Leu Gln Ser Gly Phe Arg Lys Cys 
1               5                   10          


