                         SEQUENCE LISTING

<110>  Singular Genomics Systems, Inc.
 
<120>  ENGINEERED PYROCOCCUS ENZYMES AND USES THEREOF

<130>  051385-543001WO

<150>  US 16/803,763
<151>  2020-02-27

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  775
<212>  PRT
<213>  Pyrococcus horikoshii OT3

<400>  1

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Glu Ile Lys Lys Ile Thr Ala Gln Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Ile Val Glu Thr Glu Lys Ile Gln Arg Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Thr Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Lys Leu Thr Phe Leu Ala Val Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Val Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Leu Ile Arg Val Ile Lys Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Leu Leu Gly Arg Asp Asn Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Lys Met Gly Asp Ser Leu Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Phe Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Thr Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Val Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Lys Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Gln Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Lys Arg Met Lys Glu Ser Lys Asp Pro Val Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Asp Leu Val Arg Arg Glu 
        515                 520                 525             


Leu Glu Ala Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Val Lys Asp Trp Glu Glu Val Lys Arg Arg 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asp Tyr Ile Asn Ser Lys Leu Pro Gly Val Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Ala Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Val Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Asp Val Thr Glu Lys Leu Thr Asn Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Ile Asn 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Met 
        675                 680                 685             


Ala Arg Gly Ile Lys Val Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Ser Ile Glu Glu 
705                 710                 715                 720 


Phe Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Lys 
            740                 745                 750         


Arg Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Val Gly Leu Gly Ala 
        755                 760                 765             


Trp Ile Lys Val Lys Lys Ser 
    770                 775 


<210>  2
<211>  6285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2
cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc       60

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca      120

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta      180

gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct      240

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg      300

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc      360

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta      420

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg      480

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt      540

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg      600

cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg      660

ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc      720

gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg      780

agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc      840

ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag      900

tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac      960

gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca     1020

gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat     1080

gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat     1140

aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta     1200

aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa     1260

aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt     1320

tattatgcat ttagaataaa ttttgtgtcg cccttccgcg aaattaatac gactcactat     1380

aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttttgag     1440

accttaagga ggtaaaaaat gattctggac gctgattata ttactgaaga tggtaaaccg     1500

attattcgta tttttaaaaa agaaaatggc gagttcaaag ttgaatatga ccgtaacttt     1560

cgtccgtaca tctacgcgct gttgcgcgac gatagcgcga tcgatgagat taagaaaatt     1620

accgcgcagc gtcatggtaa agttgttcgc atcgttgaaa ccgagaaaat tcaacgtaaa     1680

ttcctgggcc gcccaattga agtgtggaag ctgtacctgg agcatccgca agatgtcccg     1740

gcgatccgtg acaagattcg cgagcacccg gccgtcgtcg acattttcga atacgatatt     1800

ccgttcgcaa agcgttacct gatcgataag ggtctgaccc cgatggaggg taatgaaaag     1860

ctgacgttcc tggctgtcga tattgaaacg ttgtaccacg agggtgaaga gtttggtaag     1920

ggcccggtca ttatgatcag ctacgcggat gaagagggcg ccaaagttat cacgtggaaa     1980

aaaattgatc tgccgtacgt tgaagttgtg tccagcgagc gcgagatgat taaacgcttg     2040

attcgtgtga ttaaagaaaa agatccagac gtgatcatta cctataatgg tgacaacttt     2100

gactttccgt acttgctgaa acgtgctgag aaactgggta tcaagctgtt gctgggtcgc     2160

gataatagcg agccgaagat gcaaaaaatg ggcgatagcc tggcagtcga gatcaagggt     2220

cgcatccact ttgatctctt tccggtgatt cgtcgcacga tcaatctgcc gacctatacg     2280

ctggaagctg tctacgaggc aatctttggt aagccgaaag aaaaagtcta tgcggacgaa     2340

attgcgaaag cgtgggaaac cggcgagggc ctggagcgtg tggcaaagta ctctatggaa     2400

gatgccaaag tgacctatga actgggtcgt gagttcttcc caatggaagc ccagttggcg     2460

cgcttggtgg gccaaccggt ttgggacgtt tcccgtagca gcaccggtaa cctggttgag     2520

tggtttctgt tgcgtaaagc gtatgagcgt aatgaactgg caccgaacaa gcctgacgag     2580

aaagaatatg aacgtcgcct gcgtgaatct tacgagggtg gttacgtcaa agaaccggaa     2640

aagggtctgt gggaaggcat cgtgagcctg gatttccgta gcctgtaccc tagcatcatc     2700

atcacgcaca atgttagccc ggacaccctg aaccgcgagg gctgcgaaga gtacgacgtt     2760

gcgccgaaag tcggccatcg tttttgtaaa gacttccctg gtttcatccc aagcctgctg     2820

ggtcagctgc tggaagagag acagaaaatt aaaaaacgca tgaaagaatc gaaagatccg     2880

gttgagaaaa agctgctgga ttaccgccag cgtgccatca agattctggc taactcatat     2940

tatggctact acggttatgc taaagcgcgt tggtactgta aagagtgcgc ggagtccgtc     3000

accgcgtggg gtcgccagta tatcgatctg gtgcgtcgcg agctggaagc gcgtggtttt     3060

aaggtcctgt acatcgatac tgacggtctg tatgcaacca tccctggtgt caaagactgg     3120

gaagaggtta agcgtcgtgc actggaattt gtggactata tcaattctaa gttgccgggt     3180

gtgctggagc tggagtacga aggcttctat gcacgcggct ttttcgttac gaaaaagaaa     3240

tacgcactga tcgacgaaga gggcaagatt gtgactcgtg gtctggaaat cgttcgtcgc     3300

gactggagcg agattgcaaa agaaacccaa gctcgcgttc tggaagcaat cctgaaacat     3360

ggtaacgtcg aagaagccgt caagatcgtg aaagatgtca ccgaaaagtt gaccaactac     3420

gaagttccac cggaaaaact ggtgatttat gagcaaatca cgcgtccgat caatgaatat     3480

aaggccattg gcccgcacgt cgcggtggcc aagcgcctga tggcgcgtgg tatcaaagtg     3540

aaaccgggta tggttattgg ttacatcgtg ctgcgtggcg acggcccgat tagcaaacgt     3600

gcgatcagca ttgaagaatt tgacccgcgt aagcacaaat atgacgcgga atactatatc     3660

gagaatcaag tgctgccggc cgtggaacgc attctgaaag ctttcggcta caagcgtgaa     3720

gatttgcgct ggcagaaaac caaacaggtt ggtcttggtg cgtggatcaa ggtcaaaaag     3780

tcctaaggtt gaggtctcac cccctagcat aaccccttgg ggcctctaaa cgggtcttga     3840

ggggtttttt gcccctgaga cgcgtcaatc gagttcgtac ctaagggcga caccccctaa     3900

ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc     3960

agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg cgtttcactt     4020

ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc aaacaagggg     4080

tgttatgagc catattcagg tataaatggg ctcgcgataa tgttcagaat tggttaattg     4140

gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc     4200

atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagccat     4260

attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg     4320

tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg     4380

aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt     4440

acagatgaga tggtcagact aaactggctg acggaattta tgccacttcc gaccatcaag     4500

cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca     4560

gcgttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca     4620

gtgttcctgc gccggttgca ctcgattcct gtttgtaatt gtccttttaa cagcgatcgc     4680

gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat     4740

tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt     4800

ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt     4860

tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga     4920

taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa     4980

cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg     5040

atgctcgatg agtttttcta agcggcgcgc catcgaatgg cgcaaaacct ttcgcggtat     5100

ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatatgaaac cagtaacgtt     5160

atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca     5220

ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa     5280

ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt     5340

tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg     5400

cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc     5460

ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta     5520

tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt     5580

atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaggacgg     5640

tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc     5700

gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac     5760

tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt     5820

tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa     5880

cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc     5940

ggatatctcg gtagtgggat acgacgatac cgaagatagc tcatgttata tcccgccgtt     6000

aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca     6060

actctctcag ggccaggcgg tgaagggcaa tcagctgttg ccagtctcac tggtgaaaag     6120

aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     6180

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgactca tgaccaaaat     6240

cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagac                     6285


<210>  3
<211>  2328
<212>  DNA
<213>  Pyrococcus horikoshii OT3

<400>  3
atgattctgg acgctgatta tattactgaa gatggtaaac cgattattcg tatttttaaa       60

aaagaaaatg gcgagttcaa agttgaatat gaccgtaact ttcgtccgta catctacgcg      120

ctgttgcgcg acgatagcgc gatcgatgag attaagaaaa ttaccgcgca gcgtcatggt      180

aaagttgttc gcatcgttga aaccgagaaa attcaacgta aattcctggg ccgcccaatt      240

gaagtgtgga agctgtacct ggagcatccg caagatgtcc cggcgatccg tgacaagatt      300

cgcgagcacc cggccgtcgt cgacattttc gaatacgata ttccgttcgc aaagcgttac      360

ctgatcgata agggtctgac cccgatggag ggtaatgaaa agctgacgtt cctggctgtc      420

gatattgaaa cgttgtacca cgagggtgaa gagtttggta agggcccggt cattatgatc      480

agctacgcgg atgaagaggg cgccaaagtt atcacgtgga aaaaaattga tctgccgtac      540

gttgaagttg tgtccagcga gcgcgagatg attaaacgct tgattcgtgt gattaaagaa      600

aaagatccag acgtgatcat tacctataat ggtgacaact ttgactttcc gtacttgctg      660

aaacgtgctg agaaactggg tatcaagctg ttgctgggtc gcgataatag cgagccgaag      720

atgcaaaaaa tgggcgatag cctggcagtc gagatcaagg gtcgcatcca ctttgatctc      780

tttccggtga ttcgtcgcac gatcaatctg ccgacctata cgctggaagc tgtctacgag      840

gcaatctttg gtaagccgaa agaaaaagtc tatgcggacg aaattgcgaa agcgtgggaa      900

accggcgagg gcctggagcg tgtggcaaag tactctatgg aagatgccaa agtgacctat      960

gaactgggtc gtgagttctt cccaatggaa gcccagttgg cgcgcttggt gggccaaccg     1020

gtttgggacg tttcccgtag cagcaccggt aacctggttg agtggtttct gttgcgtaaa     1080

gcgtatgagc gtaatgaact ggcaccgaac aagcctgacg agaaagaata tgaacgtcgc     1140

ctgcgtgaat cttacgaggg tggttacgtc aaagaaccgg aaaagggtct gtgggaaggc     1200

atcgtgagcc tggatttccg tagcctgtac cctagcatca tcatcacgca caatgttagc     1260

ccggacaccc tgaaccgcga gggctgcgaa gagtacgacg ttgcgccgaa agtcggccat     1320

cgtttttgta aagacttccc tggtttcatc ccaagcctgc tgggtcagct gctggaagag     1380

agacagaaaa ttaaaaaacg catgaaagaa tcgaaagatc cggttgagaa aaagctgctg     1440

gattaccgcc agcgtgccat caagattctg gctaactcat attatggcta ctacggttat     1500

gctaaagcgc gttggtactg taaagagtgc gcggagtccg tcaccgcgtg gggtcgccag     1560

tatatcgatc tggtgcgtcg cgagctggaa gcgcgtggtt ttaaggtcct gtacatcgat     1620

actgacggtc tgtatgcaac catccctggt gtcaaagact gggaagaggt taagcgtcgt     1680

gcactggaat ttgtggacta tatcaattct aagttgccgg gtgtgctgga gctggagtac     1740

gaaggcttct atgcacgcgg ctttttcgtt acgaaaaaga aatacgcact gatcgacgaa     1800

gagggcaaga ttgtgactcg tggtctggaa atcgttcgtc gcgactggag cgagattgca     1860

aaagaaaccc aagctcgcgt tctggaagca atcctgaaac atggtaacgt cgaagaagcc     1920

gtcaagatcg tgaaagatgt caccgaaaag ttgaccaact acgaagttcc accggaaaaa     1980

ctggtgattt atgagcaaat cacgcgtccg atcaatgaat ataaggccat tggcccgcac     2040

gtcgcggtgg ccaagcgcct gatggcgcgt ggtatcaaag tgaaaccggg tatggttatt     2100

ggttacatcg tgctgcgtgg cgacggcccg attagcaaac gtgcgatcag cattgaagaa     2160

tttgacccgc gtaagcacaa atatgacgcg gaatactata tcgagaatca agtgctgccg     2220

gccgtggaac gcattctgaa agctttcggc tacaagcgtg aagatttgcg ctggcagaaa     2280

accaaacagg ttggtcttgg tgcgtggatc aaggtcaaaa agtcctaa                  2328


<210>  4
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  dideoxycytosine

<400>  4
gactcacatg aatcagtgca gcatcagatg tatgaccgaa gcggacgaag gtgcgtggac       60


<210>  5
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  dideoxycytosine

<400>  5
gtggttcatc gcgtccgata tcaaacttcg tcaagtcgaa gcggacgaag gtgcgtggac       60


<210>  6
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  dideoxycytosine

<400>  6
tactaggttg tacgatccct gcacttcagc taagcacgaa gcggacgaag gtgcgtggac       60


<210>  7
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  dideoxycytosine

<400>  7
agctaccaat atttagtttc cgagtctcag ctcatgcgaa gcggacgaag gtgcgtggac       60


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8
aaaaaaaaaa aagtccacgc accttcgtcc gcttcg                                 36


<210>  9
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  dideoxycytosine

<400>  9
ccaacttgat attaataaca ctatagacca ccgcccgaag cggacgaagg tgcgtggac        59


<210>  10
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  dideoxycytosine

<400>  10
atgattaaac tcctaagcag aaaacctacc gcgctcgaag cggacgaagg tgcgtggac        59


<210>  11
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  dideoxycytosine

<400>  11
tctttaataa cctgattcag cgaaaccaat ccgcgcgaag cggacgaagg tgcgtggac        59


<210>  12
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  dideoxycytosine

<400>  12
cggttatcgc tggcgactcc ttcgagatgg acgcccgaag cggacgaagg tgcgtggac        59


<210>  13
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  13
aaaaaaaaaa aagtccacgc accttcgtcc gcttcgggcg g                           41


<210>  14
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  14
aaaaaaaaaa aagtccacgc accttcgtcc gcttcgagcg c                           41


<210>  15
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  15
aaaaaaaaaa aagtccacgc accttcgtcc gcttcgcgcg g                           41


<210>  16
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  16
aaaaaaaaaa aagtccacgc accttcgtcc gcttcgggcg t                           41


<210>  17
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  17
ccgcc                                                                    5


<210>  18
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  18
gcgct                                                                    5


<210>  19
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  19
ccgcg                                                                    5


<210>  20
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  20
acgcc                                                                    5


<210>  21
<211>  771
<212>  PRT
<213>  Pyrococcus abyssi

<400>  21

Met Ile Ile Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Lys Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Thr Glu Val Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Glu Lys Ile Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Thr Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Glu Leu Thr Phe Leu Ala Val Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Ser Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Leu Val Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Asn Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Met Gly Asp Ser Leu Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Phe Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Ser Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Phe 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Val Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Asn Cys Lys Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Asn Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Lys Arg Met Lys Glu Ser Lys Asp Pro Val Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Asp Leu Val Arg Arg Glu 
        515                 520                 525             


Leu Glu Ser Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Ala Lys His Glu Glu Ile Lys Glu Lys Ala 
545                 550                 555                 560 


Leu Lys Phe Val Glu Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Ala Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Val Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Asp Glu Ala Val 
625                 630                 635                 640 


Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Ile Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu Ser Glu 
            660                 665                 670         


Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Lys Gly Val Lys Val Lys Pro Gly Met Val Ile Gly Tyr Ile Val Leu 
    690                 695                 700                 


Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Ala Ile Glu Glu Phe 
705                 710                 715                 720 


Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Lys Tyr Gln Lys Thr Lys Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Phe 
    770     


<210>  22
<211>  775
<212>  PRT
<213>  Pyrococcus woesei

<400>  22

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  23
<211>  775
<212>  PRT
<213>  Pyrococcus furiosus

<400>  23

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  24
<211>  775
<212>  PRT
<213>  Pyrococcus glycovorans

<400>  24

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Gln Ile 
        35                  40                  45              


Asp Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Lys Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Lys Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Leu Pro Tyr Leu Val Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Asp Met Thr Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Tyr Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Leu Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Met Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Glu Val Lys His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Lys Arg Leu Leu Asp Glu Arg Gln Glu Ile 
    450                 455                 460                 


Lys Arg Arg Met Lys Ala Ser Lys Asp Pro Ile Glu Lys Lys Met Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Phe Val Arg Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Ala Lys Pro Glu Glu Ile Lys Arg Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Glu Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Arg Gly Val Lys Val Arg Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Phe Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Ala Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Thr Gly Leu Thr Ala 
        755                 760                 765             


Trp Leu Asn Val Lys Lys Lys 
    770                 775 


<210>  25
<211>  775
<212>  PRT
<213>  Pyrococcus sp. NA2

<400>  25

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Arg Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Asp Val Arg Lys Ile Thr Ser Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Ile Asp Val Glu Lys Val Lys Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Met 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Glu Leu Thr Phe Leu Ala Val Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ala Asn Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Leu Ile Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Met Lys Leu Pro Leu Gly Arg Asp Asn Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Asp Ser Leu Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Phe Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Gln Lys Glu 
        275                 280                 285             


Lys Val Tyr Pro His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Tyr Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Lys Glu Gly Cys Gly Glu Tyr 
            420                 425                 430         


Asp Glu Ala Pro Glu Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Ser Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Lys Arg Met Lys Glu Ser Lys Asp Pro Val Glu Arg Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Glu Leu Val Arg Arg Glu 
        515                 520                 525             


Leu Glu Glu Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Glu Lys Asn Trp Glu Glu Ile Lys Arg Arg 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asn Tyr Ile Asn Ser Lys Leu Pro Gly Ile Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Thr Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Val Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Asn Tyr Glu Ile 
                645                 650                 655     


Pro Val Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu Asn 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Lys Gly Ile Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Val Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Ala Ile Glu Glu 
705                 710                 715                 720 


Phe Asp Gly Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Lys 
            740                 745                 750         


Arg Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Val Gly Leu Gly Ala 
        755                 760                 765             


Trp Leu Lys Val Lys Lys Ser 
    770                 775 


<210>  26
<211>  759
<212>  PRT
<213>  Pyrococcus sp. ST700

<400>  26

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asn Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Asp Val Arg Lys Ile Thr Ser Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Ile Asp Val Glu Lys Val Ser Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Glu Leu Ser Phe Leu Ala Val Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ala Asn Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Leu Val Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Asn Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Glu Ser Leu Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Phe Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Arg Thr Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Pro His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Val Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Tyr Glu Lys Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Gly Lys Tyr 
            420                 425                 430         


Asp Glu Ala Pro Glu Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Lys Arg Met Lys Glu Ser Lys Asp Pro Ile Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Glu Leu Val Arg Arg Glu 
        515                 520                 525             


Leu Glu Glu Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Glu Lys Asn Trp Glu Glu Ile Lys Arg Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asn Tyr Ile Asn Ser Lys Leu Pro Gly Ile Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Thr Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Asn Tyr Glu Ile 
                645                 650                 655     


Pro Val Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu Asn 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Lys Gly Ile Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Val Leu 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Ala Ile Glu Glu 
705                 710                 715                 720 


Phe Asp Gly Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Lys 
            740                 745                 750         


Lys Glu Asp Leu Arg Trp Gln 
        755                 


<210>  27
<211>  775
<212>  PRT
<213>  Pyrococcus kukulkanii

<400>  27

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Gln Ile 
        35                  40                  45              


Asp Glu Val Arg Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Ile Asp Ala Glu Lys Val Arg Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Arg Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Ser Ala Val Ile Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Glu Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Leu Pro Tyr Leu Val Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Asp Met Thr Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Tyr Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Leu Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Lys Arg Leu Leu Asp Glu Arg Gln Glu Ile 
    450                 455                 460                 


Lys Arg Lys Met Lys Ala Ser Lys Asp Pro Ile Glu Lys Lys Met Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Phe Val Arg Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Ala Lys Pro Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asp Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Arg Gly Val Lys Val Arg Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Phe Asp Leu Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Ala Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Thr Gly Leu Thr Ala 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Lys 
    770                 775 


<210>  28
<211>  776
<212>  PRT
<213>  Pyrococcus yayanosii

<400>  28

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asn Gly Lys Pro Val Val 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Ser Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Asp Ile Lys Lys Ile Thr Ala Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Val Glu Ala Glu Lys Val Arg Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Glu Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Asp Glu Phe Gly Ser Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Lys Gly Ala Lys Val Ile Thr Trp Lys Gly Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Met Lys Leu Pro Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Met Gly Asp Gly Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Ile Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Val Phe Gly Arg Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Pro Asn Glu Ile Ala Arg Ala Trp Glu Asn Cys Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Val Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Arg Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Ile Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Ile Ser Pro Asp Thr Leu Asn Lys Glu Gly Cys Asn Ser Tyr 
            420                 425                 430         


Asp Val Ala Pro Lys Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Gln Leu Leu Asp Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Arg Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Arg Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Leu Val Ser Arg Glu 
        515                 520                 525             


Leu Glu Lys Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Ser Arg Glu Trp Asp Lys Ile Lys Glu Arg 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ala Arg Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Ala Ile Leu Lys Glu Gly Asn Leu Glu Lys Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Asp Leu Lys 
            660                 665                 670         


Asp Tyr Lys Ala Val Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Arg Gly Ile Lys Val Arg Pro Gly Met Val Ile Gly Tyr Leu Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Arg Arg Ala Ile Pro Ala Glu Glu 
705                 710                 715                 720 


Phe Asp Pro Ser Arg His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Ala Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Ala Gly Leu Asp Ala 
        755                 760                 765             


Trp Leu Lys Arg Lys Ala Ser Leu 
    770                 775     


<210>  29
<211>  774
<212>  PRT
<213>  Pyrococcus sp. ST04

<400>  29

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Lys Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Glu Val Arg Lys Val Thr Ala Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Ile Asp Val Glu Lys Val Lys Lys Lys Tyr Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Glu Lys Ile Arg Glu His Pro Ala Val Val Glu Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Ile Val Pro 
        115                 120                 125             


Met Asp Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Arg Ile 
                165                 170                 175     


Asn Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Val Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Gly Ser Pro Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Asp Met Asn Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Val Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gln Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Val Gln Leu Thr Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Tyr Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Lys Tyr 
            420                 425                 430         


Asp Ile Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Val Glu Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Arg Lys Met Lys Ala Thr Ile Asn Pro Val Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Leu Val Arg Lys Glu 
        515                 520                 525             


Leu Glu Gly Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Arg Gly Asp Pro Ala Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Arg Tyr Ile Asn Glu Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Arg Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Asp Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys Glu Gly Asn Val Glu Lys Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Met Lys Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu Asn 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Val Arg Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Arg Lys Asn Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Ala Phe Gly Tyr Lys 
            740                 745                 750         


Lys Glu Asp Leu Lys Tyr Gln Lys Ser Arg Gln Val Gly Leu Gly Ala 
        755                 760                 765             


Trp Ile Lys Val Lys Lys 
    770                 


<210>  30
<211>  775
<212>  PRT
<213>  Pyrococcus sp. GB-D

<400>  30

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Gln Ile 
        35                  40                  45              


Asp Glu Val Arg Lys Ile Thr Ala Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Ile Asp Ala Glu Lys Val Arg Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Arg Leu Tyr Phe Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Ser Ala Val Ile Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Leu Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Glu Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Ile Arg Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Leu Pro Tyr Leu Val Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Pro Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Leu Gly Asp Met Thr Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala His Glu Ile Ala Glu Ala Trp Glu Thr Gly Lys Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Tyr Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Leu Val Ser Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Lys Arg Leu Leu Asp Glu Arg Gln Glu Ile 
    450                 455                 460                 


Lys Arg Lys Met Lys Ala Ser Lys Asp Pro Ile Glu Lys Lys Met Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Phe Val Arg Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Ala Lys Pro Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asp Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Lys Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Ala 
        675                 680                 685             


Ala Arg Gly Val Lys Val Arg Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Phe Asp Leu Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Ala Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Thr Gly Leu Thr Ala 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Lys 
    770                 775 


<210>  31
<211>  775
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant P.horikoshii polymerase

<400>  31

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Ile Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Val Glu Tyr Asp Arg 
            20                  25                  30          


Asn Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Ala Ile 
        35                  40                  45              


Asp Glu Ile Lys Lys Ile Thr Ala Gln Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Ile Val Glu Thr Glu Lys Ile Gln Arg Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Thr Pro 
        115                 120                 125             


Met Glu Gly Asn Glu Lys Leu Thr Phe Leu Ala Val Ala Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Val Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Lys Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Leu Ile Arg Val Ile Lys Glu Lys Asp Pro Asp Val Ile Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Pro Tyr Leu Leu Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Leu Leu Gly Arg Asp Asn Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Lys Met Gly Asp Ser Leu Ala Val Glu Ile Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Phe Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Thr Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ala Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Val Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Tyr Glu Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Glu Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Gly 
385                 390                 395                 400 


Ile Val Ser Leu Asp Phe Arg Ser Ser Ala Val Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Glu Tyr 
            420                 425                 430         


Asp Val Ala Pro Lys Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly Gln Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Lys Arg Met Lys Glu Ser Lys Asp Pro Val Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Asp Leu Val Arg Arg Glu 
        515                 520                 525             


Leu Glu Ala Arg Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly Leu 
    530                 535                 540                 


Tyr Ala Thr Ile Pro Gly Val Lys Asp Trp Glu Glu Val Lys Arg Arg 
545                 550                 555                 560 


Ala Leu Glu Phe Val Asp Tyr Ile Asn Ser Lys Leu Pro Gly Val Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Ala Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Lys Tyr Ala Leu Ile Asp Glu Glu Gly Lys Ile Val Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Ala Ile Leu Lys His Gly Asn Val Glu Glu Ala 
625                 630                 635                 640 


Val Lys Ile Val Lys Asp Val Thr Glu Lys Leu Thr Asn Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Pro Ile Asn 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Arg Leu Met 
        675                 680                 685             


Ala Arg Gly Ile Lys Val Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Lys Arg Ala Ile Ser Ile Glu Glu 
705                 710                 715                 720 


Phe Asp Pro Arg Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Lys 
            740                 745                 750         


Arg Glu Asp Leu Arg Trp Gln Lys Thr Lys Gln Val Gly Leu Gly Ala 
        755                 760                 765             


Trp Ile Lys Val Lys Lys Ser 
    770                 775 


<210>  32
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  32

Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Ser Ala Trp Gly Arg Glu Tyr Leu Glu Met Val Ile Arg 
    50                  55                  60                  


Glu Leu Glu Glu Lys Phe Gly 
65                  70      


<210>  33
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  33

Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Met Val Ile Arg 
    50                  55                  60                  


Glu Leu Glu Glu Lys Phe Gly 
65                  70      


<210>  34
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  34

Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Glu Thr Thr Ile Arg 
    50                  55                  60                  


Glu Ile Glu Glu Lys Phe Gly 
65                  70      


<210>  35
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  35

Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile Thr Met Thr Ile Lys 
    50                  55                  60                  


Glu Ile Glu Glu Lys Tyr Gly 
65                  70      


<210>  36
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  36

Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr Asp Ile Ala 
1               5                   10                  15      


Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys 
    50                  55                  60                  


Glu Leu Glu Glu Lys Phe Gly 
65                  70      


<210>  37
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  37

Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Lys Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Asp Leu Val Arg Arg 
    50                  55                  60                  


Glu Leu Glu Ala Arg Gly Phe 
65                  70      


<210>  38
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  38

Ser Pro Asp Thr Leu Asn Arg Glu Asn Cys Lys Glu Tyr Asp Val Ala 
1               5                   10                  15      


Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro 
            20                  25                  30          


Ser Leu Leu Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala 
        35                  40                  45              


Glu Ser Val Thr Ala Trp Gly Arg Gln Tyr Ile Asp Leu Val Arg Arg 
    50                  55                  60                  


Glu Leu Glu Ser Arg Gly Phe 
65                  70      


