               SEQUENCE LISTING

<110> CANVAX BIOTECH SL

<120> Novel non-immunogenic chimeric antigen receptors and uses thereof

<130> AX2100052WO

<150> EP20382160.8
<151> 2020-03-05

<160> 84

<170> BiSSAP 1.3.6

<210> 1
<211> 292
<212> PRT
<213> Homo sapiens


<220> 
<223> human native CD64 extracellular domain

<400> 1
Met Trp Phe Leu Thr Thr Leu Leu Leu Trp Val Pro Val Asp Gly Gln 
1               5                   10                  15      
Val Asp Thr Thr Lys Ala Val Ile Thr Leu Gln Pro Pro Trp Val Ser 
            20                  25                  30          
Val Phe Gln Glu Glu Thr Val Thr Leu His Cys Glu Val Leu His Leu 
        35                  40                  45              
Pro Gly Ser Ser Ser Thr Gln Trp Phe Leu Asn Gly Thr Ala Thr Gln 
    50                  55                  60                  
Thr Ser Thr Pro Ser Tyr Arg Ile Thr Ser Ala Ser Val Asn Asp Ser 
65                  70                  75                  80  
Gly Glu Tyr Arg Cys Gln Arg Gly Leu Ser Gly Arg Ser Asp Pro Ile 
                85                  90                  95      
Gln Leu Glu Ile His Arg Gly Trp Leu Leu Leu Gln Val Ser Ser Arg 
            100                 105                 110         
Val Phe Thr Glu Gly Glu Pro Leu Ala Leu Arg Cys His Ala Trp Lys 
        115                 120                 125             
Asp Lys Leu Val Tyr Asn Val Leu Tyr Tyr Arg Asn Gly Lys Ala Phe 
    130                 135                 140                 
Lys Phe Phe His Trp Asn Ser Asn Leu Thr Ile Leu Lys Thr Asn Ile 
145                 150                 155                 160 
Ser His Asn Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Arg Tyr 
                165                 170                 175     
Thr Ser Ala Gly Ile Ser Val Thr Val Lys Glu Leu Phe Pro Ala Pro 
            180                 185                 190         
Val Leu Asn Ala Ser Val Thr Ser Pro Leu Leu Glu Gly Asn Leu Val 
        195                 200                 205             
Thr Leu Ser Cys Glu Thr Lys Leu Leu Leu Gln Arg Pro Gly Leu Gln 
    210                 215                 220                 
Leu Tyr Phe Ser Phe Tyr Met Gly Ser Lys Thr Leu Arg Gly Arg Asn 
225                 230                 235                 240 
Thr Ser Ser Glu Tyr Gln Ile Leu Thr Ala Arg Arg Glu Asp Ser Gly 
                245                 250                 255     
Leu Tyr Trp Cys Glu Ala Ala Thr Glu Asp Gly Asn Val Leu Lys Arg 
            260                 265                 270         
Ser Pro Glu Leu Glu Leu Gln Val Leu Gly Leu Gln Leu Pro Thr Pro 
        275                 280                 285             
Val Trp Phe His 
    290         

<210> 2
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 R175A

<400> 2
Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Ala Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 3
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 H174E

<400> 3
Gly Thr Tyr His Cys Ser Gly Met Gly Lys Glu Arg Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 4
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 R175G

<400> 4
Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Gly Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 5
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 KHR
      changed to AAA (KHR->AAA)

<400> 5
Gly Thr Tyr His Cys Ser Gly Met Gly Ala Ala Ala Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 6
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 KHR
      changed to GGG (KHR->GGG)

<400> 6
Gly Thr Tyr His Cys Ser Gly Met Gly Gly Gly Gly Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 7
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 with
      Valine inserted after Methionine at 171 (CD64+V172)

<400> 7
Gly Thr Tyr His Cys Ser Gly Met Val Gly Lys His Arg Tyr Thr Ser 
1               5                   10                  15      
Ala Gly Ile Ser Val 
            20      

<210> 8
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 with
      Glycine inserted after Methionine at 171 (CD64+G172)

<400> 8
Gly Thr Tyr His Cys Ser Gly Met Gly Gly Lys His Arg Tyr Thr Ser 
1               5                   10                  15      
Ala Gly Ile Ser Val 
            20      

<210> 9
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 with
      Glycine inserted after Glycine at 170 (CD64+G171)

<400> 9
Gly Thr Tyr His Cys Ser Gly Gly Met Gly Lys His Arg Tyr Thr Ser 
1               5                   10                  15      
Ala Gly Ile Ser Val 
            20      

<210> 10
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN FG LOOP REGION: CD64 with
      Valine inserted after Methionine at 171 and KHR changed to AAA
      (CD64+V172 KHR->GGG)

<400> 10
Gly Thr Tyr His Cys Ser Gly Met Val Gly Gly Gly Gly Tyr Thr Ser 
1               5                   10                  15      
Ala Gly Ile Ser Val 
            20      

<210> 11
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130Y

<400> 11
Ala Leu Arg Cys His Ala Trp Lys Asp Tyr Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 12
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130F

<400> 12
Ala Leu Arg Cys His Ala Trp Lys Asp Phe Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 13
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130F

<400> 13
Ala Leu Arg Cys His Ala Trp Lys Asp Trp Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 14
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130A

<400> 14
Ala Leu Arg Cys His Ala Trp Lys Asp Ala Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 15
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130S

<400> 15
Ala Leu Arg Cys His Ala Trp Lys Asp Ser Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 16
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> PARTIAL SEQUENCE OF CD64 MUTATED IN HYDROPHOBIC POCKET: CD64
      K130G

<400> 16
Ala Leu Arg Cys His Ala Trp Lys Asp Gly Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 17
<211> 21
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF CD8 ALPHA TRANSMEMBRANE REGION

<400> 17
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      
Ser Leu Val Ile Thr 
            20      

<210> 18
<211> 27
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF CD8 ALPHA TRANSMEMBRANE REGION WITH PALMITOYLATION
      SEQUENCE (LYC)

<400> 18
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      
Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg 
            20                  25          

<210> 19
<211> 41
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE

<400> 19
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 20
<211> 156
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A DIGLYCINE LINKER TO FULL CD3 ZETA INTRACELLULAR STIMULATION
      DOMAIN - (CD28 con CD3z complete)

<400> 20
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Arg Val Lys Phe Ser 
        35                  40                  45              
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
    50                  55                  60                  
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
65                  70                  75                  80  
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
                85                  90                  95      
Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            100                 105                 110         
Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
        115                 120                 125             
Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
    130                 135                 140                 
Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 155     

<210> 21
<211> 124
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A DIGLYCINE LINKER TO TRUNCATED CD3 ZETA INTRACELLULAR
      STIMULATION DOMAIN COMPRISING TWO ITAMS - (CD28 con CD3z 2ITAMs)

<400> 21
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Arg Val Lys Phe Ser 
        35                  40                  45              
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
    50                  55                  60                  
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
65                  70                  75                  80  
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
                85                  90                  95      
Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
            100                 105                 110         
Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
        115                 120                 

<210> 22
<211> 200
<212> PRT
<213> Homo sapiens


<220> 
<223> HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH DILEUCINE INT
      ERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING A TRIGLYCINE 
      LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN OF HUMAN 4-1BB
       FUSED USING A DIGLYCINE LINKER TO FULL CD3 ZETA INTRACELLULAR

<400> 22
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Gly Gly Gly Lys Arg Gly Arg Lys 
        35                  40                  45              
Lys Gly Gly Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
    50                  55                  60                  
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
65                  70                  75                  80  
Gly Gly Cys Glu Leu Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
                85                  90                  95      
Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            100                 105                 110         
Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        115                 120                 125             
Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
    130                 135                 140                 
Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
145                 150                 155                 160 
Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                165                 170                 175     
Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            180                 185                 190         
His Met Gln Ala Leu Pro Pro Arg 
        195                 200 

<210> 23
<211> 195
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A TRIGLYCINE LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN
      OF HUMAN OX40 FUSED USING A DIGLYCINE LINKER TO FULL CD3 ZETA

<400> 23
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Gly Gly Gly Arg Arg Asp Gln Arg 
        35                  40                  45              
Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr 
    50                  55                  60                  
Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
65                  70                  75                  80  
Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                85                  90                  95      
Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            100                 105                 110         
Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        115                 120                 125             
Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    130                 135                 140                 
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
145                 150                 155                 160 
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                165                 170                 175     
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            180                 185                 190         
Pro Pro Arg 
        195 

<210> 24
<211> 204
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A TRIGLYCINE LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN
      OF HUMAN ICOS FUSED USING A DIGLYCINE LINKER TO FULL CD3 ZETA

<400> 24
Cys Asp Ile Cys Ser Cys Asp Glx Arg Ser Lys Arg Ser Arg Gly Gly 
1               5                   10                  15      
His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
            20                  25                  30          
Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
        35                  40                  45              
Gly Gly Gly Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His 
    50                  55                  60                  
Asp Pro Asn Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys 
65                  70                  75                  80  
Lys Ser Arg Leu Thr Asp Val Thr Leu Gly Gly Arg Val Lys Phe Ser 
                85                  90                  95      
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
            100                 105                 110         
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        115                 120                 125             
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
    130                 135                 140                 
Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
145                 150                 155                 160 
Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                165                 170                 175     
Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            180                 185                 190         
Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        195                 200                 

<210> 25
<211> 168
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH D
      ILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING A
       TRIGLYCINE LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN OF
       HUMAN 4-1BB FUSED USING A DIGLYCINE LINKER TO TRUNCATED CD3 ZETA

<400> 25
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Gly Gly Gly Lys Arg Gly Arg Lys 
        35                  40                  45              
Lys Gly Gly Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
    50                  55                  60                  
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
65                  70                  75                  80  
Gly Gly Cys Glu Leu Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
                85                  90                  95      
Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            100                 105                 110         
Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        115                 120                 125             
Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu 
    130                 135                 140                 
Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
145                 150                 155                 160 
Glu Ile Gly Met Lys Gly Glu Arg 
                165             

<210> 26
<211> 163
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A TRIGLYCINE

<400> 26
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Gly Gly Gly Arg Arg Asp Gln Arg 
        35                  40                  45              
Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr 
    50                  55                  60                  
Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
65                  70                  75                  80  
Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                85                  90                  95      
Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            100                 105                 110         
Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        115                 120                 125             
Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    130                 135                 140                 
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
145                 150                 155                 160 
Gly Glu Arg 
            

<210> 27
<211> 164
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH D
      ILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING A
       TRIGLYCINE LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN OF
       HUMAN ICOS FUSED USING A DIGLYCINE LINKER TO TRUNCATED CD3 ZETA

<400> 27
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Gly Gly Gly Cys Trp Leu Thr Lys 
        35                  40                  45              
Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn Gly Glu Tyr Met Phe 
    50                  55                  60                  
Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg Leu Thr Asp Val Thr 
65                  70                  75                  80  
Leu Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                85                  90                  95      
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            100                 105                 110         
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        115                 120                 125             
Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
    130                 135                 140                 
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
145                 150                 155                 160 
Lys Gly Glu Arg 
                

<210> 28
<211> 113
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF HUMAN CD28 INTRACELLULAR CO-STIMULATION DOMAIN WITH
      DILEUCINE INTERNALIZATION MOTIF CHANGED TO DIGLYCINE FUSED USING
      A TRIGLYCINE LINKER TO FULL INTRACELLULAR CO-STIMULATION DOMAIN
      OF HUMAN ICOS FUSED USING A DIGLYCINE LINKER TO TRUNCATED

<400> 28
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         
Arg 
    

<210> 29
<211> 81
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF TRUNCATED HUMAN CD3 ZETA INTRACELLULAR STIMULATION
      DOMAIN COMPRISING TWO ITAMS

<400> 29
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg 
    

<210> 30
<211> 157
<212> PRT
<213> Homo sapiens


<220> 
<223> SEQUENCE OF FULL INTRACELLULAR CO-STIMULATION DOMAIN OF HUMAN
      4-1BB FUSED USING A DIGLYCINE LINKER TO FULL CD3 ZETA
      INTRACELLULAR STIMULATION DOMAIN

<400> 30
Lys Arg Gly Arg Lys Lys Gly Gly Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Arg Val Lys Phe 
        35                  40                  45              
Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
    50                  55                  60                  
Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
65                  70                  75                  80  
Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg 
                85                  90                  95      
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
            100                 105                 110         
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
        115                 120                 125             
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
    130                 135                 140                 
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 155         

<210> 31
<211> 489
<212> DNA
<213> Homo sapiens


<220> 
<223> NUCLEOTIDE SEQUENCE OF TCRVB6.7 PROMOTER THAT MAY BE USED FOR
      EXPRESSION OF CAR-T

<400> 31
gagggaaccc atgtgatggg acaatcccgt tgggcacatg tgagtggggg aatggaggag      60

gctggggcat gaatggggat ggcagagggg accctgactt ggaggaaaga caatgagctc     120

atcccatggt gcctggtgtt gggggcactg ttggcacatc ctacagaacg tgctcagcag     180

acagaggagc ggctgtggga tgagaaggtg aactcggaga tgcagcgtga ggcctccaag     240

tccagacagc atgggagccc aaagcgatgt cccatgcaat aacgttgttt acaaggagct     300

tataaatatt taaagtagtc acccaagtgt ggtctaatat aaatcctgtg ttcctgaggt     360

catgcagatt gagagaggaa gtgatgtcac tgtgggaact tccgtgtaag gacggggcgt     420

ccctcctcct ctgctcctgc tcacagtgat cctgatctgg taaagctccc atcctgccct     480

gaccctgcc                                                             489


<210> 32
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 32
Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Arg Tyr Thr Ser Ala 
1               5                   10                  15      
Gly Ile Ser Val 
            20  

<210> 33
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 33
Cys Ser Gly Met Gly Lys His Arg Tyr 
1               5                   

<210> 34
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 34
Arg Tyr Thr Ser Ala Gly Ile Ser Val 
1               5                   

<210> 35
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 35
Met Gly Lys His Arg Tyr Thr Ser Ala 
1               5                   

<210> 36
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 36
Met Val Gly Lys His Arg Tyr Thr Ser 
1               5                   

<210> 37
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 37
Val Gly Lys His Arg Tyr Thr Ser Ala 
1               5                   

<210> 38
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 38
Met Gly Gly Lys His Arg Tyr Thr Ser 
1               5                   

<210> 39
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 39
Gly Gly Lys His Arg Tyr Thr Ser Ala 
1               5                   

<210> 40
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 40
Gly Thr Tyr His Cys Ser Gly Gly Met 
1               5                   

<210> 41
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 41
Tyr His Cys Ser Gly Gly Met Gly Lys 
1               5                   

<210> 42
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 42
Tyr His Cys Ser Gly Met Val Gly Gly 
1               5                   

<210> 43
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 43
Ser Gly Met Val Gly Gly Gly Gly Tyr 
1               5                   

<210> 44
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 44
Cys Ser Gly Met Gly Lys Glu Arg Tyr 
1               5                   

<210> 45
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 45
Met Gly Lys Glu Arg Tyr Thr Ser Ala 
1               5                   

<210> 46
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 46
Lys Glu Arg Tyr Thr Ser Ala Gly Ile 
1               5                   

<210> 47
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 47
Cys Ser Gly Met Gly Lys His Ala Tyr 
1               5                   

<210> 48
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 48
Met Gly Lys His Ala Tyr Thr Ser Ala 
1               5                   

<210> 49
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 49
Lys His Ala Tyr Thr Ser Ala Gly Ile 
1               5                   

<210> 50
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 50
Thr Ser Ala Gly Ile Ser Val Thr Val 
1               5                   

<210> 51
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 51
Cys Ser Gly Met Gly Ala Ala Ala Tyr 
1               5                   

<210> 52
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 52
Tyr His Cys Ser Gly Met Gly Ala Ala 
1               5                   

<210> 53
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 53
Cys Ser Gly Met Gly Gly Gly Gly Tyr 
1               5                   

<210> 54
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 54
Thr Ser Ala Gly Ile Ser Val Thr Val 
1               5                   

<210> 55
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 55
Ala Leu Arg Cys His Ala Trp Lys Asp Lys Leu Val Tyr Asn Val Leu 
1               5                   10                  15      
Tyr Tyr Arg Asn 
            20  

<210> 56
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 56
Lys Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 57
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 57
His Ala Trp Lys Asp Ser Leu Val Tyr 
1               5                   

<210> 58
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 58
Asp Ser Leu Val Tyr Asn Val Leu Tyr 
1               5                   

<210> 59
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 59
Ser Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 60
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 60
Trp Lys Asp Ser Leu Val Tyr Asn Val 
1               5                   

<210> 61
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 61
Gly Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 62
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 62
Trp Lys Asp Gly Leu Val Tyr Asn Val 
1               5                   

<210> 63
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 63
His Ala Trp Lys Asp Gly Leu Val Tyr 
1               5                   

<210> 64
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 64
Ala Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 65
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 65
Asp Ala Leu Val Tyr Asn Val Leu Tyr 
1               5                   

<210> 66
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 66
Arg Cys His Ala Trp Lys Asp Ala Leu 
1               5                   

<210> 67
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 67
Trp Lys Asp Ala Leu Val Tyr Asn Val 
1               5                   

<210> 68
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 68
His Ala Trp Lys Asp Ala Leu Val Tyr 
1               5                   

<210> 69
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 69
His Ala Trp Lys Asp Trp Leu Val Tyr 
1               5                   

<210> 70
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 70
Trp Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 71
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 71
Cys His Ala Trp Lys Asp Trp Leu Val 
1               5                   

<210> 72
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 72
Trp Lys Asp Trp Leu Val Tyr Asn Val 
1               5                   

<210> 73
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 73
Tyr Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 74
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 74
His Ala Trp Lys Asp Tyr Leu Val Tyr 
1               5                   

<210> 75
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 75
Cys His Ala Trp Lys Asp Tyr Leu Val 
1               5                   

<210> 76
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 76
Trp Lys Asp Tyr Leu Val Tyr Asn Val 
1               5                   

<210> 77
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 77
Cys Ser Gly Met Gly Lys His Gly Tyr 
1               5                   

<210> 78
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 78
Met Gly Lys His Gly Tyr Thr Ser Ala 
1               5                   

<210> 79
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 79
Phe Leu Val Tyr Asn Val Leu Tyr Tyr 
1               5                   

<210> 80
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 80
Leu Val Tyr Asn Val Leu Tyr Tyr Arg 
1               5                   

<210> 81
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 81
Cys His Ala Trp Lys Asp Phe Leu Val 
1               5                   

<210> 82
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 82
Trp Lys Asp Phe Leu Val Tyr Asn Val 
1               5                   

<210> 83
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 83
His Ala Trp Lys Asp Phe Leu Val Tyr 
1               5                   

<210> 84
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> Epitopes of mutated human CD64

<400> 84
Met Gly Lys His Arg Tyr 
1               5       

