                         SEQUENCE LISTING

<110>  Rijksuniversiteit Groningen
 
<120>  Engineered PlyAB nanopores and uses thereof

<130>  P125675PC00

<140>  PCT/NL2021/050167
<141>  2021-03-11

<150>  EP 20162431.9
<151>  2020-03-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  1
gattaacgcc agtggtcgct c                                                 21


<210>  2
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  2
cccacggact atcccagta                                                    19


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  3
taatacgact cactataggg                                                   20


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
cttcaggtcg aaggcttcgt cctccg                                            26


<210>  5
<211>  145
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PlyA with His tag

<400>  5

Met Ala Tyr Ala Gln Trp Val Ile Ile Ile Ile His Asn Val Gly Ser 
1               5                   10                  15      


Lys Asp Val Lys Ile Lys Asn Leu Lys Pro Ser Trp Gly Lys Leu His 
            20                  25                  30          


Ala Asp Gly Asp Lys Asp Thr Glu Val Ser Ala Ser Lys Tyr Glu Gly 
        35                  40                  45              


Thr Val Ile Lys Pro Asp Glu Lys Leu Gln Ile Asn Ala Cys Gly Arg 
    50                  55                  60                  


Ser Asp Ala Ala Glu Gly Thr Thr Gly Thr Phe Asp Leu Val Asp Pro 
65                  70                  75                  80  


Ala Asp Gly Asp Lys Gln Val Arg His Phe Tyr Trp Asp Cys Pro Trp 
                85                  90                  95      


Gly Ser Lys Thr Asn Thr Trp Thr Val Ser Gly Ser Asn Thr Lys Trp 
            100                 105                 110         


Met Ile Glu Tyr Ser Gly Gln Asn Leu Asp Ser Gly Ala Leu Gly Thr 
        115                 120                 125             


Ile Thr Val Asp Thr Leu Lys Lys Gly Ser Ala His His His His His 
    130                 135                 140                 


His 
145 


<210>  6
<211>  449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyA with restriction sites

<400>  6
ccatggctta tgcacagtgg gttattatta tcattcacaa tgtgggatcc aaggatgtga       60

agattaaaaa tctgaagccg tcctggggaa aattgcatgc agatggtgat aaggataccg      120

aggtatcggc gtcgaagtac gagggcactg taattaaacc agatgaaaaa ttacagatta      180

acgcctgtgg tcgctcggat gcggcagagg gcacgaccgg gactttcgac ctggttgatc      240

cggcagacgg cgacaagcag gtccgccact tttactggga ttgtccgtgg gggtctaaga      300

caaatacatg gacggtttcc ggtagcaaca cgaaatggat gatcgaatac tccggtcaga      360

acttagacag tggtgcgctg gggaccatta ccgtagatac gctgaagaag gggtcagcgc      420

atcatcacca ccaccattga taagcttcg                                        449


<210>  7
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PlyB with His tag

<400>  7

Met Ala Ser Gln Ala Gly Asp Thr Leu Asn Asp Val Ile Gln Asp Pro 
1               5                   10                  15      


Thr Arg Arg Asn Lys Leu Ile Asn Asp Asn Asn Leu Leu Lys Gly Ile 
            20                  25                  30          


Ile Met Gly Arg Asp Gly Pro Val Pro Ser Ser Arg Glu Leu Ile Val 
        35                  40                  45              


Arg Pro Asp Thr Leu Arg Ala Ile Ile Asn Asn Arg Ala Thr Ile Glu 
    50                  55                  60                  


Thr Thr Thr Met Glu Ala Glu Phe Thr Glu Thr Leu Met Glu Ser Asn 
65                  70                  75                  80  


Tyr Asn Ser Ala Ser Val Lys Val Ser Ala Pro Phe Ile Thr Ala Asn 
                85                  90                  95      


Ser Glu Tyr Ser Glu Ser Ser Ser Phe Lys Asn Thr Glu Thr Glu Lys 
            100                 105                 110         


Ser Met Tyr Thr Ser Ser Arg Tyr Leu Phe Pro Gln Gly Arg Ile Asp 
        115                 120                 125             


Phe Thr Thr Pro Asp Ser Gly Phe Asp Asp Val Ile Lys Leu Ser Pro 
    130                 135                 140                 


Gln Phe Thr Ser Gly Val Gln Ala Ala Leu Ala Lys Ala Thr Gly Thr 
145                 150                 155                 160 


Glu Lys Arg Glu Ala Leu Gln Asn Leu Phe Gln Glu Tyr Gly His Val 
                165                 170                 175     


Phe Arg Thr Lys Val His Ile Gly Gly Val Leu Ser Ala His Thr Met 
            180                 185                 190         


Glu Thr Phe Ser Arg Ser Glu Asn Glu Thr Glu Val Lys Gln Asp Val 
        195                 200                 205             


Lys Ala Gly Leu Glu Gly Ala Val Lys Gly Trp Gly Gly Gly Ala Thr 
    210                 215                 220                 


Ala Gly His Gly Asn Thr Gln Gly Thr Ile Thr Thr Ser Gln Asn Arg 
225                 230                 235                 240 


Lys Leu Asn Val Lys Tyr Ile Val Asn Gly Gly Asp Tyr Thr Lys Ile 
                245                 250                 255     


Gln Asn Thr Glu Glu Trp Val Ala Ser Thr Asn Gln Ser Glu His Trp 
            260                 265                 270         


Arg Val Ile Glu Val Thr Glu Val Thr Ala Val Ala Asp Leu Leu Pro 
        275                 280                 285             


Gln Pro Ile Arg Gly Gln Val Lys Asp Leu Leu Lys Pro Leu Leu Gly 
    290                 295                 300                 


Lys Trp Val Asp Val Glu Lys Val Pro Gly Leu Glu Ser Leu Pro Val 
305                 310                 315                 320 


Ser Val Tyr Arg Pro Lys Gly Ala Ile Pro Ala Gly Trp Phe Trp Leu 
                325                 330                 335     


Gly Asp Thr Ala Asp Ala Ser Lys Ala Leu Leu Val Lys Pro Thr Leu 
            340                 345                 350         


Pro Ala Arg Ser Gly Arg Asn Pro Ala Leu Thr Ser Leu His Gln Gly 
        355                 360                 365             


Ser Gly Met Thr Glu Gln Pro Phe Val Asp Leu Pro Gln Tyr Gln Tyr 
    370                 375                 380                 


Leu Ser Thr Tyr Phe Gly Ser Phe Ala His Asp Thr Pro Pro Gly Ser 
385                 390                 395                 400 


Thr Leu Arg Gly Leu Arg Pro Asp His Val Leu Pro Gly Arg Tyr Glu 
                405                 410                 415     


Met His Gly Asp Thr Ile Ser Thr Ala Val Tyr Val Thr Arg Pro Val 
            420                 425                 430         


Asp Val Pro Phe Pro Glu Asp Glu Cys Phe Asp Leu Lys Ser Leu Val 
        435                 440                 445             


Arg Val Lys Leu Pro Gly Ser Gly Asn Pro Pro Lys Pro Arg Ser Ala 
    450                 455                 460                 


Leu Lys Lys Ser Met Val Leu Phe Asp Ser Gly Glu Lys Gly Ser Ala 
465                 470                 475                 480 


His His His His His His 
                485     


<210>  8
<211>  1472
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyB with His tag

<400>  8
ccatggcttc ccaagcgggt gatactttga atgacgtgat ccaagatccc acccggagaa       60

ataagttaat caatgacaat aacttactta aggggatcat aatggggaga gacgggccag      120

tcccttcatc gagagaattg atagtccgtc cggacactct gcgggcaata atcaacaacc      180

gcgccacgat agagactacc actatggagg ccgaatttac ggaaacgctt atggagtcga      240

attacaattc agcgtcggtg aaagttagcg cacctttcat aacagcaaac tcagaatata      300

gcgaatcctc gtccttcaag aacacggaga ctgaaaagtc catgtacacc agttctagat      360

atttgtttcc gcaaggtcgc attgatttta caaccccaga ctcggggttc gatgacgtta      420

ttaagctatc acctcaattc acaagcggag ttcaagcagc attggcaaag gccacaggga      480

ccgaaaaacg ggaagcgctt cagaacctgt tccaagagta tggacacgtg ttcagaacta      540

aagttcatat cgggggcgtt ttaagtgctc atacaatgga gacgttcagc agaagcgaga      600

atgagacaga agtcaaacaa gacgtcaaag ctgggcttga gggcgctgtt aaggggtggg      660

gaggtggcgc aaccgcggga catggcaaca ctcaagggac cattactact agccaaaacc      720

gcaaattgaa tgtaaaatac atcgtgaatg gaggcgatta cacaaaaatt cagaataccg      780

aggagtgggt agcgtctacg aaccagtccg aacattggcg tgtaatcgaa gttaccgagg      840

taaccgctgt tgcagattta ctgccacagc ctattcgtgg gcaagtcaag gatcttctta      900

aaccgttgct tgggaagtgg gtagatgtag agaaagtgcc tggtttagag tcacttcctg      960

tatccgtata tcgcccgaaa ggggccattc ccgccggatg gttttggtta ggcgatactg     1020

ctgacgcttc caaagccctt cttgtcaaac caacattacc ggctcggtct ggacgtaacc     1080

ctgcgcttac gagccttcac caggggtcag gtatgacgga acaaccgttc gttgatttgc     1140

ctcagtatca atacctttcg acatatttcg gaagttttgc gcatgatacc ccgccaggat     1200

cgactcttcg tggtttgcgc ccggatcacg tcctgccagg ccggtacgaa atgcatggag     1260

acaccatatc tacggcggtc tatgtaacac gtcccgtgga tgtaccattc ccggaggacg     1320

aatgcttcga cctgaagagc ttggttcgtg ttaagttgcc cgggtccggg aatcctccaa     1380

aaccccgtag cgcgttgaag aaatcaatgg tgctgttcga ttcaggtgaa aaagggtcag     1440

cgcatcatca ccaccaccat tgataagctt cg                                   1472


