<110>    HieraBio Inc.

<120>    Composition comprising a cardiac stem cell for the prevention and
         treatment of ischemic disease

<130>    MPCT21-026

<150>    KR 10-2020-0032823
<151>    2020-03-17

<150>    KR 10-2021-0034721
<151>    2021-03-17

<160>    12

<170>    KoPatentIn 3.0

<210>    1
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    VEGF forward primer


<400>    1
ggagtacccc gatgagatag agt                                                 23


<210>    2
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    VEGF reverse primer


<400>    2
ctatgtgctg gctttggtga g                                                   21


<210>    3
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PDGF-B forward primer


<400>    3
tggagtcgag tcggaaagct                                                     20


<210>    4
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PDGF-B reverse primer


<400>    4
gaagttggca ttggtgcgat                                                     20


<210>    5
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bFGF forward primer


<400>    5
gcgacccaca cgtcaaacta                                                     20


<210>    6
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    bFGF reverse primer


<400>    6
cagccgtcca tcttccttca                                                     20


<210>    7
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ang-1 forward primer


<400>    7
ctcgctgcca ttctgactac ac                                                  22


<210>    8
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Ang-1 reverse primer


<400>    8
gacagttgcc atcgtgttct g                                                   21


<210>    9
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    beta-actin forward primer


<400>    9
agatcaagat cattgctcct cct                                                 23


<210>    10
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    beta-actin reverse primer


<400>    10
acgcagctca gtaacagtcc                                                     20


<210>    11
<211>    823
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    HIF-1 protein


<400>    11
Met Glu Gly Ala Gly Gly Glu Asn Glu Lys Lys Asn Arg Met Ser Ser
  1               5                  10                  15 

Glu Arg Arg Lys Glu Lys Ser Arg Asp Ala Ala Arg Ser Arg Arg Ser
             20                  25                  30 

Lys Glu Ser Glu Val Phe Tyr Glu Leu Ala His Gln Leu Pro Leu Pro
         35                  40                  45 

His Asn Val Ser Ser His Leu Asp Lys Ala Ser Val Met Arg Leu Thr
     50                  55                  60 

Ile Ser Tyr Leu Arg Val Arg Lys Leu Leu Gly Ala Gly Asp Leu Asp
 65                  70                  75                  80 

Ile Glu Asp Glu Met Lys Ala Gln Met Asn Cys Phe Tyr Leu Lys Ala
                 85                  90                  95 

Leu Asp Gly Phe Val Met Val Leu Thr Asp Asp Gly Asp Met Ile Tyr
            100                 105                 110 

Ile Ser Asp Asn Val Asn Lys Tyr Met Gly Leu Thr Gln Phe Glu Leu
        115                 120                 125 

Thr Gly His Ser Val Phe Asp Phe Thr His Pro Cys Asp His Glu Glu
    130                 135                 140 

Met Arg Glu Met Leu Thr His Arg Asn Gly Pro Val Arg Lys Gly Lys
145                 150                 155                 160 

Glu Gln Asn Thr Gln Arg Ser Phe Phe Leu Arg Met Lys Cys Thr Leu
                165                 170                 175 

Thr Ser Arg Gly Arg Thr Met Asn Ile Lys Ser Ala Thr Trp Lys Val
            180                 185                 190 

Leu His Cys Thr Gly His Ile His Val Tyr Asp Thr Ser Ser Asn Gln
        195                 200                 205 

Pro Gln Cys Gly Tyr Lys Lys Pro Pro Met Thr Cys Leu Val Leu Ile
    210                 215                 220 

Cys Glu Pro Ile Pro His Pro Ser Asn Ile Glu Ile Pro Leu Asp Ser
225                 230                 235                 240 

Lys Thr Phe Leu Ser Arg His Ser Leu Asp Met Lys Phe Ser Tyr Cys
                245                 250                 255 

Asp Glu Arg Ile Thr Glu Leu Met Gly Tyr Glu Pro Glu Glu Leu Leu
            260                 265                 270 

Gly Arg Ser Ile Tyr Glu Tyr Tyr His Ala Leu Asp Ser Asp His Leu
        275                 280                 285 

Thr Lys Thr His His Asp Met Phe Thr Lys Gly Gln Val Thr Thr Gly
    290                 295                 300 

Gln Tyr Arg Met Leu Ala Lys Arg Gly Gly Tyr Val Trp Val Glu Thr
305                 310                 315                 320 

Gln Ala Thr Val Ile Tyr Asn Thr Lys Asn Ser Gln Pro Gln Cys Ile
                325                 330                 335 

Val Cys Val Asn Tyr Val Val Ser Gly Ile Ile Gln His Asp Leu Ile
            340                 345                 350 

Phe Ser Leu Gln Gln Thr Glu Ser Val Leu Lys Pro Val Glu Ser Ser
        355                 360                 365 

Asp Met Lys Met Thr Gln Leu Phe Thr Lys Val Glu Ser Glu Asp Thr
    370                 375                 380 

Ser Cys Leu Phe Asp Lys Leu Lys Lys Glu Pro Asp Ala Leu Thr Leu
385                 390                 395                 400 

Leu Ala Pro Ala Ala Gly Asp Thr Ile Ile Ser Leu Asp Phe Gly Ser
                405                 410                 415 

Asp Asp Thr Glu Thr Glu Asp Gln Gln Leu Glu Asp Val Pro Leu Tyr
            420                 425                 430 

Asn Asp Val Met Phe Pro Ser Ser Asn Glu Lys Leu Asn Ile Asn Leu
        435                 440                 445 

Ala Met Ser Pro Leu Pro Ala Ser Glu Thr Pro Lys Pro Leu Arg Ser
    450                 455                 460 

Ser Ala Asp Pro Ala Leu Asn Gln Glu Val Ala Leu Lys Leu Glu Ser
465                 470                 475                 480 

Ser Pro Glu Ser Leu Gly Leu Ser Phe Thr Met Pro Gln Ile Gln Asp
                485                 490                 495 

Gln Pro Ala Ser Pro Ser Asp Gly Ser Thr Arg Gln Ser Ser Pro Glu
            500                 505                 510 

Pro Asn Ser Pro Ser Glu Tyr Cys Phe Asp Val Asp Ser Asp Met Val
        515                 520                 525 

Asn Val Phe Lys Leu Glu Leu Val Glu Lys Leu Phe Ala Glu Asp Thr
    530                 535                 540 

Glu Ala Lys Asn Pro Phe Ser Ala Gln Asp Thr Asp Leu Asp Leu Glu
545                 550                 555                 560 

Met Leu Ala Pro Tyr Ile Pro Met Asp Asp Asp Phe Gln Leu Arg Ser
                565                 570                 575 

Phe Asp Gln Leu Ser Pro Leu Glu Ser Asn Ser Pro Ser Pro Pro Ser
            580                 585                 590 

Val Ser Thr Val Thr Gly Phe Gln Gln Thr Gln Leu Gln Lys Pro Thr
        595                 600                 605 

Ile Thr Val Thr Ala Thr Ala Thr Ala Thr Thr Asp Glu Ser Lys Ala
    610                 615                 620 

Val Thr Lys Asp Asn Ile Glu Asp Ile Lys Ile Leu Ile Ala Ser Pro
625                 630                 635                 640 

Pro Ser Thr Gln Val Pro Gln Glu Met Thr Thr Ala Lys Ala Ser Ala
                645                 650                 655 

Tyr Ser Gly Thr His Ser Arg Thr Ala Ser Pro Asp Arg Ala Gly Lys
            660                 665                 670 

Arg Val Ile Glu Lys Thr Asp Lys Ala His Pro Arg Ser Leu Asn Leu
        675                 680                 685 

Ser Val Thr Leu Asn Gln Arg Asn Thr Val Pro Glu Glu Glu Leu Asn
    690                 695                 700 

Pro Lys Thr Ile Ala Leu Gln Asn Ala Gln Arg Lys Arg Lys Met Glu
705                 710                 715                 720 

His Asp Gly Ser Leu Phe Gln Ala Ala Gly Ile Gly Thr Leu Leu Gln
                725                 730                 735 

Gln Pro Gly Asp Arg Ala Pro Thr Met Ser Leu Ser Trp Lys Arg Val
            740                 745                 750 

Lys Gly Tyr Ile Ser Ser Glu Gln Asp Gly Met Glu Gln Lys Thr Ile
        755                 760                 765 

Phe Leu Ile Pro Ser Asp Leu Ala Cys Arg Leu Leu Gly Gln Ser Met
    770                 775                 780 

Asp Glu Ser Gly Leu Pro Gln Leu Thr Ser Tyr Asp Cys Glu Val Asn
785                 790                 795                 800 

Ala Pro Ile Gln Gly Ser Arg Asn Leu Leu Gln Gly Glu Glu Leu Leu
                805                 810                 815 

Arg Ala Leu Asp Gln Val Asn
            820            


<210>    12
<211>    696
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    constitutively active HIF-1 protein


<400>    12
Met Glu Gly Ala Gly Gly Ala Asn Asp Lys Lys Lys Ile Ser Ser Glu
  1               5                  10                  15 

Arg Arg Lys Glu Lys Ser Arg Asp Ala Ala Arg Ser Arg Arg Ser Lys
             20                  25                  30 

Glu Ser Glu Val Phe Tyr Glu Leu Ala His Gln Leu Pro Leu Pro His
         35                  40                  45 

Asn Val Ser Ser His Leu Asp Lys Ala Ser Val Met Arg Leu Thr Ile
     50                  55                  60 

Ser Tyr Leu Arg Val Arg Lys Leu Leu Asp Ala Gly Asp Leu Asp Ile
 65                  70                  75                  80 

Glu Asp Asp Met Lys Ala Gln Met Asn Cys Phe Tyr Leu Lys Ala Leu
                 85                  90                  95 

Asp Gly Phe Val Met Val Leu Thr Asp Asp Gly Asp Met Ile Tyr Ile
            100                 105                 110 

Ser Asp Asn Val Asn Lys Tyr Met Gly Leu Thr Gln Phe Glu Leu Thr
        115                 120                 125 

Gly His Ser Val Phe Asp Phe Thr His Pro Cys Asp His Glu Glu Met
    130                 135                 140 

Arg Glu Met Leu Thr His Arg Asn Gly Leu Val Lys Lys Gly Lys Glu
145                 150                 155                 160 

Gln Asn Thr Gln Arg Ser Phe Phe Leu Arg Met Lys Cys Thr Leu Thr
                165                 170                 175 

Ser Arg Gly Arg Thr Met Asn Ile Lys Ser Ala Thr Trp Lys Val Leu
            180                 185                 190 

His Cys Thr Gly His Ile His Val Tyr Asp Thr Asn Ser Asn Gln Pro
        195                 200                 205 

Gln Cys Gly Tyr Lys Lys Pro Pro Met Thr Cys Leu Val Leu Ile Cys
    210                 215                 220 

Glu Pro Ile Pro His Pro Ser Asn Ile Glu Ile Pro Leu Asp Ser Lys
225                 230                 235                 240 

Thr Phe Leu Ser Arg His Ser Leu Asp Met Lys Phe Ser Tyr Cys Asp
                245                 250                 255 

Glu Arg Ile Thr Glu Leu Met Gly Tyr Glu Pro Glu Glu Leu Leu Gly
            260                 265                 270 

Arg Ser Ile Tyr Glu Tyr Tyr His Ala Leu Asp Ser Asp His Leu Thr
        275                 280                 285 

Lys Thr His His Asp Met Phe Thr Lys Gly Gln Val Thr Thr Gly Gln
    290                 295                 300 

Tyr Arg Met Leu Ala Lys Arg Gly Gly Tyr Val Trp Val Glu Thr Gln
305                 310                 315                 320 

Ala Thr Val Ile Tyr Asn Thr Lys Asn Ser Gln Pro Gln Cys Ile Val
                325                 330                 335 

Cys Val Asn Tyr Val Val Ser Gly Ile Ile Gln His Asp Leu Ile Phe
            340                 345                 350 

Ser Leu Gln Gln Thr Glu Cys Val Leu Lys Pro Val Glu Ser Ser Asp
        355                 360                 365 

Met Lys Met Thr Gln Leu Phe Thr Lys Val Glu Ser Glu Asp Thr Ser
    370                 375                 380 

Ser Leu Phe Asp Lys Leu Lys Phe Tyr Val Asp Ser Asp Met Val Asn
385                 390                 395                 400 

Glu Phe Lys Leu Glu Leu Val Glu Lys Leu Phe Ala Glu Asp Thr Glu
                405                 410                 415 

Ala Lys Asn Pro Phe Ser Thr Gln Asp Thr Asp Leu Asp Leu Glu Met
            420                 425                 430 

Leu Ala Pro Tyr Ile Thr Asp Asp Asp Phe Gln Leu Arg Ser Phe Asp
        435                 440                 445 

Gln Leu Ser Pro Leu Glu Ser Ser Ser Ala Ser Pro Glu Ser Ala Ser
    450                 455                 460 

Pro Gln Ser Thr Val Thr Val Phe Gln Gln Thr Gln Ile Gln Glu Pro
465                 470                 475                 480 

Thr Ala Asn Ala Thr Thr Thr Thr Ala Thr Thr Asp Glu Leu Lys Thr
                485                 490                 495 

Val Thr Lys Asp Arg Met Glu Asp Ile Lys Ile Leu Ile Ala Ser Pro
            500                 505                 510 

Ser Pro Thr His Ile His Lys Glu Thr Thr Ser Ala Thr Ser Ser Gln
        515                 520                 525 

Tyr Arg Asp Thr Gln Ser Arg Thr Ala Ser Pro Asn Arg Ala Gly Lys
    530                 535                 540 

Gly Val Ile Glu Gln Thr Glu Lys Ser His Pro Arg Ser Pro Asn Val
545                 550                 555                 560 

Leu Ser Val Ala Leu Ser Gln Arg Thr Thr Val Pro Glu Glu Glu Leu
                565                 570                 575 

Asn Pro Lys Ile Leu Ala Leu Gln Asn Ala Gln Arg Lys Arg Lys Met
            580                 585                 590 

Glu His Asp Gly Ser Leu Phe Gln Ala Val Gly Ile Gly Thr Leu Leu
        595                 600                 605 

Gln Gln Pro Asp Asp His Ala Ala Thr Thr Ser Leu Ser Trp Lys Arg
    610                 615                 620 

Val Lys Gly Cys Lys Ser Ser Glu Gln Asn Gly Met Glu Gln Lys Thr
625                 630                 635                 640 

Ile Ile Leu Ile Pro Ser Asp Leu Ala Cys Arg Leu Leu Gly Gln Ser
                645                 650                 655 

Met Asp Glu Ser Gly Leu Pro Gln Leu Thr Ser Tyr Asp Cys Glu Val
            660                 665                 670 

Asn Ala Pro Ile Gln Gly Ser Arg Asn Leu Leu Gln Gly Glu Glu Leu
        675                 680                 685 

Leu Arg Ala Leu Asp Gln Val Asn
    690                 695    
