                         SEQUENCE LISTING

<110>  Flagship Pioneering, Inc.
 
<120>  BIFUNCTIONAL MOLECULES AND METHODS OF USING THEREOF

<130>  127250-5006-WO

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MALAT1 targetting ASO

<400>  1
cguuaacuag gcuuua                                                       16


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XIST targeting ASO

<400>  2
ggaagggaat cagcaggtat                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HSP70 targeting ASO

<400>  3
tcttgggccg aggctactga                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC targeting ASO

<400>  4
cctggggctg gtgcattttc                                                   20


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MALAT1 targetting ASO

<400>  5
cgttaactag gcttta                                                       16


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HSP70 targetting ASO

<400>  6
tcttgggccg aggctactga                                                   20


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO1

<400>  7
gtaagtggaa ttccagttg                                                    19


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO2

<400>  8
agctttagac cacgaggcac                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO3

<400>  9
aagctttaga ccacgaggca                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO4

<400>  10
gaagctttag accacgaggc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO5

<400>  11
cgaagcttta gaccacgagg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO6

<400>  12
ccgaagcttt agaccacgag                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO7

<400>  13
gccgaagctt tagaccacga                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO8

<400>  14
tgccgaagct ttagaccacg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO9

<400>  15
gtgccgaagc tttagaccac                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO10

<400>  16
tgtgccgaag ctttagacca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO11

<400>  17
ttgtgccgaa gctttagacc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO12

<400>  18
cttgtgccga agctttagac                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO13

<400>  19
ccttgtgccg aagctttaga                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO14

<400>  20
cccttgtgcc gaagctttag                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO15

<400>  21
gcccttgtgc cgaagcttta                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO16

<400>  22
ggcccttgtg ccgaagcttt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO17

<400>  23
gacacggatt ctgtatttgt                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO18

<400>  24
aggccacgag gtttctccca                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO19

<400>  25
catctcaaat aatggagacc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO20

<400>  26
tttagaccac gaggcacgtc                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO21

<400>  27
agtaaacaga gatctcaacc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO22

<400>  28
ctggatggaa gtatacacca                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO23

<400>  29
tatcacagaa ctaggctgtg                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO24

<400>  30
cattgaagga tcatggtcat                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO25

<400>  31
ttatagacta gattggccag                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO26

<400>  32
tttaatctcc ttctggccaa                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO27

<400>  33
cagcagtcat ccaaatattc                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO28

<400>  34
aagctccagc cacagaaaca                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO29

<400>  35
actcctcctt tccagtgcag                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO30

<400>  36
ccacttaaca aatccctctg                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO31

<400>  37
gccactctta accaggcaaa                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO32

<400>  38
agtcataccc gtaagtggaa                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1 ASO33

<400>  39
cacagtcata cccgtaagtg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO1

<400>  40
tttcttcttt ctctcgccgg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO2

<400>  41
aaggtttcag aggtgatgag                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO3

<400>  42
cggagacgca cttagtgaac                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO4

<400>  43
gtcctaacac ctctagagac                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO5

<400>  44
ttcattcact ctcagagatc                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MYC ASO6

<400>  45
gcatgaatac gttagaaagg                                                   20


<210>  46
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SCN1A ASO1

<400>  46
agtaagactg gggttgtt                                                     18


<210>  47
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNGAP1 ASO1

<400>  47
taggaagtat caagctgtg                                                    19


<210>  48
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNGAP1 ASO2

<400>  48
atcacctcct atagctcct                                                    19


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNGAP1 ASO3

<400>  49
catctctcac cacgtttgg                                                    19


<210>  50
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SYNGAP1 ASO4

<400>  50
aatcttgcca tcacccaca                                                    19


<210>  51
<211>  169
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Cy5-labeled IVT RNA

<400>  51
ccuugaaauc caugacgcag ggagaauugc gucauuuaaa gccuaguuaa cgcauuuacu       60

aaacgcagac gaaaauggaa agauuaauug ggagugguag gaugaaacaa uuuggagaag      120

auagaaguuu gaaguggaaa acuggaagac agaaguacgg gaaggcgaa                  169


<210>  52
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO1

<400>  52
gtcgaataaa ccagtatc                                                     18


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO2

<400>  53
gatccaagta aatcagcacg acc                                               23


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO3

<400>  54
ataggtggtc tctgatggtc                                                   20


<210>  55
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO4

<400>  55
agtaagactg gggttgtt                                                     18


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO5

<400>  56
gtatgtgtac cgcattgttt                                                   20


<210>  57
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO6

<400>  57
gagccagtca caaattcaga tcaccc                                            26


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO7

<400>  58
ttgtcgtaag tgttgcaaac                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non PVT1 targeting ASO8

<400>  59
actgaattct gacaaatgac                                                   20


<210>  60
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scramble A (ScrA)

<400>  60
agaggtggcg tggtag                                                       16


<210>  61
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Scramble B (ScrB)

<400>  61
aacacgtcta tacgcc                                                       16


<210>  62
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1- ASO1

<400>  62
gtaagtggaa ttccagttg                                                    19


<210>  63
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr1

<400>  63
tgaagtggaa ttccagttg                                                    19


<210>  64
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr2

<400>  64
gtaagaggta ttccagttg                                                    19


<210>  65
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr3

<400>  65
gtaagtggaa ctatcgttg                                                    19


<210>  66
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr4

<400>  66
gtaagtggaa ttccattgg                                                    19


<210>  67
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr5

<400>  67
taggatggaa ttccagttg                                                    19


<210>  68
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr6

<400>  68
gtaagatagg ttccagttg                                                    19


<210>  69
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr7

<400>  69
gtaagtggaa cattcgttg                                                    19


<210>  70
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVT1-ASO1-scr8

<400>  70
gtaagtggaa ttccatggt                                                    19


<210>  71
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTK

<400>  71

Lys Asn Ala Pro Ser Thr Ala Gly Leu Gly Tyr Gly Ser Trp Glu Ile 
1               5                   10                  15      


Asp Pro Lys Asp Leu Thr Phe Leu Lys Glu Leu Gly Thr Gly Gln Phe 
            20                  25                  30          


Gly Val Val Lys Tyr Gly Lys Trp Arg Gly Gln Tyr Asp Val Ala Ile 
        35                  40                  45              


Lys Met Ile Lys Glu Gly Ser Met Ser Glu Asp Glu Phe Ile Glu Glu 
    50                  55                  60                  


Ala Lys Val Met Met Asn Leu Ser His Glu Lys Leu Val Gln Leu Tyr 
65                  70                  75                  80  


Gly Val Cys Thr Lys Gln Arg Pro Ile Phe Ile Ile Thr Glu Tyr Met 
                85                  90                  95      


Ala Asn Gly Cys Leu Leu Asn Tyr Leu Arg Glu Met Arg His Arg Phe 
            100                 105                 110         


Gln Thr Gln Gln Leu Leu Glu Met Cys Lys Asp Val Cys Glu Ala Met 
        115                 120                 125             


Glu Tyr Leu Glu Ser Lys Gln Phe Leu His Arg Asp Leu Ala Ala Arg 
    130                 135                 140                 


Asn Cys Leu Val Asn Asp Gln Gly Val Val Lys Val Ser Asp Phe Gly 
145                 150                 155                 160 


Leu Ser Arg Tyr Val Leu Asp Asp Glu Tyr Thr Ser Ser Val Gly Ser 
                165                 170                 175     


Lys Phe Pro Val Arg Trp Ser Pro Pro Glu Val Leu Met Tyr Ser Lys 
            180                 185                 190         


Phe Ser Ser Lys Ser Asp Ile Trp Ala Phe Gly Val Leu Met Trp Glu 
        195                 200                 205             


Ile Tyr Ser Leu Gly Lys Met Pro Tyr Glu Arg Phe Thr Asn Ser Glu 
    210                 215                 220                 


Thr Ala Glu His Ile Ala Gln Gly Leu Arg Leu Tyr Arg Pro His Leu 
225                 230                 235                 240 


Ala Ser Glu Lys Val Tyr Thr Ile Met Tyr Ser Cys Trp His Glu Lys 
                245                 250                 255     


Ala Asp Glu Arg Pro Thr Phe Lys Ile Leu Leu Ser Asn Ile Leu Asp 
            260                 265                 270         


Val Met Asp Glu Glu Ser 
        275             


<210>  72
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence

<400>  72

Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp Ile Asp Tyr 
1               5                   10                  15      


Lys Asp Asp Asp Asp Lys 
            20          


