                         SEQUENCE LISTING

<110>  The Broad Institute, Inc.
       Massachusetts Institute of Technology
 
<120>  Sequencing-Based Population Scale Screening

<130>  BROD-5130WP

<150>  63/004,456
<151>  2020-04-02

<150>  63,032,697
<151>  2020-05-31

<160>  198   

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  n is g, c, or a

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  n is a or c

<220>
<221>  misc_feature
<222>  (59)..(59)
<223>  n is a, c, or t

<220>
<221>  misc_feature
<222>  (60)..(60)
<223>  n is c or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  n is t or a

<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  n is g or a

<220>
<221>  misc_feature
<222>  (63)..(63)
<223>  n is a or g

<220>
<221>  misc_feature
<222>  (64)..(64)
<223>  n is t, g, or a

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  n is a, g, or c

<400>  1
aacgctgaag cgctgggggc aaattgtgca atttgcggcc aatgtttgta atcagcnnnn       60

nnnnnccaag gaaattttgg ggaccaggaa ctaatcagac aaggaactga ttacaaac        118


<210>  2
<211>  289
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2
tccagatgag gatgaagaag aaggtgattg tgaagaagaa gagtttgagc catcaactca       60

atatgagtat ggtactgaag atgattacca aggtaaacct ttggaatttg gtgccacttc      120

tgctgctctt caacctgaag aagagcaaga agaagattgg ttagatgatg atagtcaaca      180

aactgttggt caacaagacg gcagtgagga caatcagaca actactattc aaacaattgt      240

tgaggttcaa cctcaattag agatggaact tacaccagtt gttcagact                  289


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
tccagatgag gatgaagaag a                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4
agtctgaaca actggtgtaa g                                                 21


<210>  5
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
agagcagcag aagtggcaca ggtgattgtg aagaagaaga g                           41


<210>  6
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
tcaacctgaa gaagagcaag aactgattgt cctcactgcc                             40


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
ctcatattga gttgatggct ca                                                22


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
acaaactgtt ggtcaacaag ac                                                22


<210>  9
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
agagcagcag aagtggcaca gcttaggtga ttgtgaagaa gaagag                      46


<210>  10
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
agagcagcag aagtggcaca gcttacacta ggtgattgtg aagaagaaga g                51


<210>  11
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
agagcagcag aagtggcaca gcttacactc aatgatccga ggtgattgtg aagaagaaga       60

g                                                                       61


<210>  12
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12
agagcagcag aagtggcaca gcttacactc aatgatccga tatggagccc agaggtgatt       60

gtgaagaaga agag                                                         74


<210>  13
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
tggctactac cgaagagcta ccagacgaat tcgtggtggt gacggtaaaa tgaaagatct       60

cagtccaaga tggtatttct actacctagg aactgggcca gaagctggac ttccctatgg      120

tgctaacaaa gacggcatca tatgggttgc aactgaggga gccttgaata caccaaaaga      180

tcacattggc acccgcaatc ctgctaacaa tgctgca                               217


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
tggctactac cgaagagct                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
tgcagcattg ttagcaggat                                                   20


<210>  16
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
tctggcccag ttcctaggta gtccagacga attcgtggtg g                           41


<210>  17
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
ggactgagat ctttcatttt accgt                                             25


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19
actgagggag ccttgaatac a                                                 21


<210>  20
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20
tctggcccag ttcctaggta gtagcttcca gacgaattcg tggtgg                      46


<210>  21
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21
tctggcccag ttcctaggta gtagcttaca ctccagacga attcgtggtg g                51


<210>  22
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22
tctggcccag ttcctaggta gtagcttaca ctcaatgatc cgccagacga attcgtggtg       60

g                                                                       61


<210>  23
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
tctggcccag ttcctaggta gtagcttaca ctcaatgatc cgatatggag cccagccaga       60

cgaattcgtg gtgg                                                         74


<210>  24
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  24
agacggcatc atatgggttg caagagccgg gtgccaatgt gatct                       45


<210>  25
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  25
agacggcatc atatgggttg caagagccgt tgcgggtgcc aatgtgatct                  50


<210>  26
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  26
agacggcatc atatgggttg caagagccgt tgcagggatt aacgggtgcc aatgtgatct       60


<210>  27
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27
agacggcatc atatgggttg caagagccgt tgcagggatt aaggaacacg tccaccgggt       60

gccaatgtga tct                                                          73


<210>  28
<211>  229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28
aacacaagct ttcggcagac gtggtccaga acaaacccaa ggaaattttg gggaccagga       60

actaatcaga caaggaactg attacaaaca ttggccgcaa attgcacaat ttgcccccag      120

cgcttcagcg ttcttcggaa tgtcgcgcat tggcatggaa gtcacacctt cgggaacgtg      180

gttgacctac acaggtgcca tcaaattgga tgacaaagat ccaaatttc                  229


<210>  29
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29
aacacaagct ttcggcag                                                     18


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30
gaaatttgga tctttgtcat cc                                                22


<210>  31
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31
tgcggccaat gtttgtaatc agccaaggaa attttgggga c                           41


<210>  32
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33
ttccttgtct gattagttc                                                    19


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34
accttcggga acgtggtt                                                     18


<210>  35
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  35
tgcggccaat gtttgtaatc agagcttcca aggaaatttt ggggac                      46


<210>  36
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  36
tgcggccaat gtttgtaatc agagcttaca ctccaaggaa attttgggga c                51


<210>  37
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  37
tgcggccaat gtttgtaatc agagcttaca ctcaatgatc cgccaaggaa attttgggga       60

c                                                                       61


<210>  38
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  38
tgcggccaat gtttgtaatc agagcttaca ctcaatgatc cgatatggag cccagccaag       60

gaaattttgg ggac                                                         74


<210>  39
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  39
aacgctgaag cgctgggggc aaattgtgca atttgcggcc aatgtttgta atcagagctt       60

acactccaag gaaattttgg ggaccaggaa ctaatcagac aaggaactga ttacaaac        118


<210>  40
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  40
cagatgtccc                                                              10


<210>  41
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  41
atcgctgtcg                                                              10


<210>  42
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  42
gattatgtcc                                                              10


<210>  43
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  43
tgcttagtcc                                                              10


<210>  44
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  44
ttagaagtcc                                                              10


<210>  45
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  45
ctttccgtcc                                                              10


<210>  46
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  n is a, c, g, or t

<400>  46
nnnnnnnnnn nn                                                           12


<210>  47
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - barcoded forward inner primer where N can be any 
       nucleotide and collectively denote a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  47
tctggcccag ttcctaggta gtnnnnnnnn nnccagacga attcgtggtg g                51


<210>  48
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - barcoded backward inner primer

<400>  48
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-F3)

<400>  49
tggctactac cgaagagct                                                    19


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-B3)

<400>  50
tgcagcattg ttagcaggat                                                   20


<210>  51
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-LF)

<400>  51
ggactgagat ctttcatttt accgt                                             25


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (Bl-LB)

<400>  52
actgagggag ccttgaatac a                                                 21


<210>  53
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer-PCR B-fwd-10 primer

<400>  53
acactctttc cctacacgac gctcttccga tctccgtctt tgttagcacc ataggg           56


<210>  54
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer PCR-B-rev-12

<400>  54
tgactggagt tcagacgtgt gctcttccga tctaccatct tggactgaga tctttc           56


<210>  55
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - pool-specific forward barcoding primer, N's can be 
       any nucleotide and collectively denote a specific barcode 
       sequence


<220>
<221>  misc_feature
<222>  (30)..(39)
<223>  n is a, c, g, or t

<400>  55
aatgatacgg cgaccaccga gatctacacn nnnnnnnnna cactctttcc ctacacgacg       60

ct                                                                      62


<210>  56
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - poll specific reverse barcoding primer, N can be any 
       nucleotide and collectively N's denote a specific barcode 
       sequence


<220>
<221>  misc_feature
<222>  (25)..(34)
<223>  n is a, c, g, or t

<400>  56
caagcagaag acggcatacg agatnnnnnn nnnngtgact ggagttcaga cgtgtgct         58


<210>  57
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  57
tccagatgag gatgaagaag a                                                 21


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  58
agtctgaaca actggtgtaa g                                                 21


<210>  59
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence


<220>
<221>  misc_feature
<222>  (20)..(29)
<223>  n is a, c, g, or t

<400>  59
agagcagcag aagtggcacn nnnnnnnnna ggtgattgtg aagaagaaga g                51


<210>  60
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  60
tcaacctgaa gaagagcaag aactgattgt cctcactgcc                             40


<210>  61
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  61
ctcatattga gttgatggct ca                                                22


<210>  62
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  62
acaaactgtt ggtcaacaag ac                                                22


<210>  63
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  63
tggctactac cgaagagct                                                    19


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  64
tgcagcattg ttagcaggat                                                   20


<210>  65
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  65
tctggcccag ttcctaggta gtnnnnnnnn nnccagacga attcgtggtg g                51


<210>  66
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  66
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  67
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  67
ggactgagat ctttcatttt accgt                                             25


<210>  68
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  68
actgagggag ccttgaatac a                                                 21


<210>  69
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  69
acactctttc cctacacgac gctcttccga tctccgtctt tgttagcacc ataggg           56


<210>  70
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  70
tgactggagt tcagacgtgt gctcttccga tctaccatct tggactgaga tctttc           56


<210>  71
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  71
aacacaagct ttcggcag                                                     18


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  72
gaaatttgga tctttgtcat cc                                                22


<210>  73
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  73
tgcggccaat gtttgtaatc agnnnnnnnn nnccaaggaa attttgggga c                51


<210>  74
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  74
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  75
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  75
ttccttgtct gattagttc                                                    19


<210>  76
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  76
accttcggga acgtggtt                                                     18


<210>  77
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  77
acactctttc cctacacgac gctcttccga tctaacgctg aagcgctggg ggcaaa           56


<210>  78
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer, where relevant N is any nucleotide and 
       collectively denotes a specific barcode seuqence

<400>  78
tgactggagt tcagacgtgt gctcttccga tctgtttgta atcagttcct tgtctg           56


<210>  79
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  79
tccagatgag gatgaagaag a                                                 21


<210>  80
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  80
tccagatgag gatgaagaag a                                                 21


<210>  81
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-F3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  81
tccagatgag gatgaagaag a                                                 21


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  82
cttacaccag ttgttcagac t                                                 21


<210>  83
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  83
agtctgaaca actggtgtaa g                                                 21


<210>  84
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-B3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  84
agtctgaaca actggtgtaa g                                                 21


<210>  85
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  85
aggtgattgt gaagaagaag ag                                                22


<210>  86
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  86
aggtgattgt gaagaagaag ag                                                22


<210>  87
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-FIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence


<220>
<221>  misc_feature
<222>  (20)..(29)
<223>  n is a, c, g, or t

<400>  87
agagcagcag aagtggcacn nnnnnnnnna ggtgattgtg aagaagaaga g                51


<210>  88
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  88
tcaacctgaa gaagagcaag aa                                                22


<210>  89
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  89
tcaacctgaa gaagagcaag aa                                                22


<210>  90
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  90
tcaacctgaa gaagagcaag aactgattgt cctcactgcc                             40


<210>  91
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  91
ggcagtgagg acaatcag                                                     18


<210>  92
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  92
ctgattgtcc tcactgcc                                                     18


<210>  93
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  93
tcaacctgaa gaagagcaag aactgattgt cctcactgcc                             40


<210>  94
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  94
tgagccatca actcaatatg ag                                                22


<210>  95
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  95
ctcatattga gttgatggct ca                                                22


<210>  96
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-LF), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  96
ctcatattga gttgatggct ca                                                22


<210>  97
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  97
acaaactgtt ggtcaacaag ac                                                22


<210>  98
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  98
acaaactgtt ggtcaacaag ac                                                22


<210>  99
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (A-LB), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  99
acaaactgtt ggtcaacaag ac                                                22


<210>  100
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  100
ggtactgaag atgattacca agg                                               23


<210>  101
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  101
ccttggtaat catcttcagt acc                                               23


<210>  102
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq A f wd 10), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  102
acactctttc cctacacgac gctcttccga tctccttggt aatcatcttc agtacc           56


<210>  103
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  103
aggacaatca g                                                            11


<210>  104
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  104
aggacaatca g                                                            11


<210>  105
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq A r ev 12), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  105
tgactggagt tcagacgtgt gctcttccga tctaggacaa tcagttcttg ctcttc           56


<210>  106
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  106
gaagagcaag aa                                                           12


<210>  107
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  107
ttcttgctct tc                                                           12


<210>  108
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq A r ev 12), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  108
tgactggagt tcagacgtgt gctcttccga tctaggacaa tcagttcttg ctcttc           56


<210>  109
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  109
tggctactac cgaagagct                                                    19


<210>  110
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  110
tggctactac cgaagagct                                                    19


<210>  111
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-F3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  111
tggctactac cgaagagct                                                    19


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  112
atcctgctaa caatgctgca                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  113
tgcagcattg ttagcaggat                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-B3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  114
tgcagcattg ttagcaggat                                                   20


<210>  115
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  115
ccagacgaat tcgtggtgg                                                    19


<210>  116
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  116
ccagacgaat tcgtggtgg                                                    19


<210>  117
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-FIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  117
tctggcccag ttcctaggta gtnnnnnnnn nnccagacga attcgtggtg g                51


<210>  118
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  118
agacggcatc atatgggttg ca                                                22


<210>  119
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  119
agacggcatc atatgggttg ca                                                22


<210>  120
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  120
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  121
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  121
agatcacatt ggcacccg                                                     18


<210>  122
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  122
cgggtgccaa tgtgatct                                                     18


<210>  123
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  123
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  124
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  124
acggtaaaat gaaagatctc agtcc                                             25


<210>  125
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  125
ggactgagat ctttcatttt accgt                                             25


<210>  126
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-LF), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  126
ggactgagat ctttcatttt accgt                                             25


<210>  127
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  127
actgagggag ccttgaatac a                                                 21


<210>  128
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  128
actgagggag ccttgaatac a                                                 21


<210>  129
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (B-LB), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  129
actgagggag ccttgaatac a                                                 21


<210>  130
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  130
ccctatggtg ctaacaaaga cgg                                               23


<210>  131
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  131
ccgtctttgt tagcaccata ggg                                               23


<210>  132
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq B f wd 10), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  132
acactctttc cctacacgac gctcttccga tctccgtctt tgttagcacc ataggg           56


<210>  133
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  133
gaaagatctc agtccaagat ggt                                               23


<210>  134
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  134
accatcttgg actgagatct ttc                                               23


<210>  135
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq B r ev 12), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  135
tgactggagt tcagacgtgt gctcttccga tctaccatct tggactgaga tctttc           56


<210>  136
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  136
aacacaagct ttcggcag                                                     18


<210>  137
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  137
aacacaagct ttcggcag                                                     18


<210>  138
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-F3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  138
aacacaagct ttcggcag                                                     18


<210>  139
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  139
ggatgacaaa gatccaaatt tc                                                22


<210>  140
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  140
gaaatttgga tctttgtcat cc                                                22


<210>  141
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-B3), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  141
gaaatttgga tctttgtcat cc                                                22


<210>  142
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  142
ccaaggaaat tttggggac                                                    19


<210>  143
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  143
ccaaggaaat tttggggac                                                    19


<210>  144
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-FIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  144
tgcggccaat gtttgtaatc agnnnnnnnn nnccaaggaa attttgggga c                51


<210>  145
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  145
cgcattggca tggaagtcac                                                   20


<210>  146
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  146
cgcattggca tggaagtcac                                                   20


<210>  147
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  147
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  148
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  148
ctacacaggt gccatcaaa                                                    19


<210>  149
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  149
tttgatggca cctgtgtag                                                    19


<210>  150
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-BIP), where relevant N is any nucleotide 
       and collectively denotes a specific barcode sequence

<400>  150
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  151
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  151
gaactaatca gacaaggaa                                                    19


<210>  152
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  152
ttccttgtct gattagttc                                                    19


<210>  153
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-LF), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  153
ttccttgtct gattagttc                                                    19


<210>  154
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  154
accttcggga acgtggtt                                                     18


<210>  155
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  155
accttcggga acgtggtt                                                     18


<210>  156
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (C-LB), where relevant N is any nucleotide and
       collectively denotes a specific barcode sequence

<400>  156
accttcggga acgtggtt                                                     18


<210>  157
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  157
tttgccccca gcgcttcagc gtt                                               23


<210>  158
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  158
aacgctgaag cgctgggggc aaa                                               23


<210>  159
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq C f wd 10), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  159
acactctttc cctacacgac gctcttccga tctaacgctg aagcgctggg ggcaaa           56


<210>  160
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Binding Site in SARS CoV-2 Genome, where 
       relevant N is any nucleotide and collectively denotes a specific 
       barcode sequence

<400>  160
cagacaagga actgattaca aac                                               23


<210>  161
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - Primer Sequence (5'-3'), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  161
gtttgtaatc agttccttgt ctg                                               23


<210>  162
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer (miseq C r ev 11), where relevant N is any 
       nucleotide and collectively denotes a specific barcode sequence

<400>  162
tgactggagt tcagacgtgt gctcttccga tctgtttgta atcagttcct tgtctg           56


<210>  163
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - barcoded FIP primer, N can be any nucletoide and 
       collectively denotes a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  163
tctggcccag ttcctaggta gtnnnnnnnn nnccagacga attcgtggtg g                51


<210>  164
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - BIP primer

<400>  164
agacggcatc atatgggttg cacgggtgcc aatgtgatct                             40


<210>  165
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer F3

<400>  165
tggctactac cgaagagct                                                    19


<210>  166
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer B3

<400>  166
tgcagcattg ttagcaggat                                                   20


<210>  167
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer LF

<400>  167
ggactgagat ctttcatttt accgt                                             25


<210>  168
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer LB

<400>  168
actgagggag ccttgaatac a                                                 21


<210>  169
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer B_fwd_10

<400>  169
acactctttc cctacacgac gctcttccga tctccgtctt tgttagcacc ataggg           56


<210>  170
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - FIP primer B_rev_12

<400>  170
tgactggagt tcagacgtgt gctcttccga tctaccatct tggactgaga tctttc           56


<210>  171
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer D501

<400>  171
aatgatacgg cgaccaccga gatctacact atagcctaca ctctttccct acacgacgct       60


<210>  172
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer D701

<400>  172
caagcagaag acggcatacg agatcgagta atgtgactgg agttcagacg tgtgct           56


<210>  173
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - - barcoded C-FIP primers, N can be any nucleotide, 
       collectively N's denote a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  173
tgcggccaat gtttgtaatc agnnnnnnnn nnccaaggaa attttgggga c                51


<210>  174
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer C-BIP

<400>  174
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  175
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-F3 primer

<400>  175
aacacaagct ttcggcag                                                     18


<210>  176
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-B3 primer

<400>  176
gaaatttgga tctttgtcat cc                                                22


<210>  177
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-LF primer

<400>  177
ttccttgtct gattagttc                                                    19


<210>  178
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-LB primer

<400>  178
accttcggga acgtggtt                                                     18


<210>  179
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer, PCR-C-forward primer

<400>  179
acactctttc cctacacgac gctcttccga tctaacgctg aagcgctggg ggcaaa           56


<210>  180
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer, PCR-C-reverse primer

<400>  180
tgactggagt tcagacgtgt gctcttccga tctgtttgta atcagttcct tgtctg           56


<210>  181
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - , pool-specific forward barcoding primer, N can be 
       any nucleotide, where collectively N's denotes a specific barcode
       sequence


<220>
<221>  misc_feature
<222>  (30)..(39)
<223>  n is a, c, g, or t

<400>  181
aatgatacgg cgaccaccga gatctacacn nnnnnnnnna cactctttcc ctacacgacg       60

ct                                                                      62


<210>  182
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - pool-specific reverse barcoding primer, N can be any 
       nucleotide, where collectively N's denotes a specific barcode 
       sequence


<220>
<221>  misc_feature
<222>  (25)..(34)
<223>  n is a, c, g, or t

<400>  182
caagcagaag acggcatacg agatnnnnnn nnnngtgact ggagttcaga cgtgtgct         58


<210>  183
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - barcoded C-FIP primers, N can be any nucleotide, 
       collectively N's denote a specific barcode sequence


<220>
<221>  misc_feature
<222>  (23)..(32)
<223>  n is a, c, g, or t

<400>  183
tgcggccaat gtttgtaatc agnnnnnnnn nnccaaggaa attttgggga c                51


<210>  184
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - primer C-BIP

<400>  184
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  185
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-F3 primer

<400>  185
aacacaagct ttcggcag                                                     18


<210>  186
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-B3 primer

<400>  186
gaaatttgga tctttgtcat cc                                                22


<210>  187
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-LF primer

<400>  187
ttccttgtct gattagttc                                                    19


<210>  188
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - C-LB primer

<400>  188
accttcggga acgtggtt                                                     18


<210>  189
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer, PCR-C-forward primer

<400>  189
acactctttc cctacacgac gctcttccga tctaacgctg aagcgctggg ggcaaa           56


<210>  190
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - PCR primer, PCR-C-reverse primer

<400>  190
tgactggagt tcagacgtgt gctcttccga tctgtttgta atcagttcct tgtctg           56


<210>  191
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - pool-specific forward barcoding primer, N can be any 
       nucleotide, where collectively N's denotes a specific barcode 
       sequence


<220>
<221>  misc_feature
<222>  (30)..(39)
<223>  n is a, c, g, or t

<400>  191
aatgatacgg cgaccaccga gatctacacn nnnnnnnnna cactctttcc ctacacgacg       60

ct                                                                      62


<210>  192
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - pool-specific reverse barcoding primer, N can be any 
       nucleotide, where collectively N's denotes a specific barcode 
       sequence


<220>
<221>  misc_feature
<222>  (25)..(34)
<223>  n is a, c, g, or t

<400>  192
caagcagaag acggcatacg agatnnnnnn nnnngtgact ggagttcaga cgtgtgct         58


<210>  193
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR forward primer for N-gene

<400>  193
cacattggca cccgcaatc                                                    19


<210>  194
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR reverse primer for N-gene

<400>  194
gaggaacgag aagaggcttg                                                   20


<210>  195
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR probe sequence, 5' FAM label, 3' BBQ label

<400>  195
acttcctcaa ggaacaacat tgcca                                             25


<210>  196
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR forward primer MS2 gene

<400>  196
tgctcgcgga tacccg                                                       16


<210>  197
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR reverse primer MS2 gene

<400>  197
aacttgcgtt ctcgagcgat                                                   20


<210>  198
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - RT-qPCR probe MS2 gene, 5' FAM label, 3' BBQ label

<400>  198
acctcgggtt tccgtcttgc tcgt                                              24


