                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CELL

<130>  P120145PCT

<140>  PCT/GB2021/050866
<141>  2021-04-08

<150>  GB 2005331.0
<151>  2020-04-09

<150>  GB 2017358.9
<151>  2020-11-02

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  58
<212>  PRT
<213>  Homo sapiens

<400>  1

Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met Trp Pro 
1               5                   10                  15      


Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu Asp Ala 
            20                  25                  30          


Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu 
        35                  40                  45              


Glu Lys Gly Arg Leu Gly Asp Leu Trp Val 
    50                  55              


<210>  2
<211>  188
<212>  PRT
<213>  Homo sapiens

<400>  2

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  3
<211>  169
<212>  PRT
<213>  Homo sapiens

<400>  3

Thr Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Gly 
1               5                   10                  15      


Trp Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp Val Leu 
            20                  25                  30          


Tyr Cys Lys Gln Phe Phe Asn Arg His Cys Gln Arg Gly Gly Leu Leu 
        35                  40                  45              


Gln Phe Glu Ala Asp Lys Thr Ala Lys Glu Glu Ser Leu Phe Pro Val 
    50                  55                  60                  


Pro Pro Ser Lys Glu Thr Ser Ala Glu Ser Gln Val Ser Glu Asn Ile 
65                  70                  75                  80  


Phe Gln Thr Gln Pro Leu Asn Pro Ile Leu Glu Asp Asp Cys Ser Ser 
                85                  90                  95      


Thr Ser Gly Phe Pro Thr Gln Glu Ser Phe Thr Met Ala Ser Cys Thr 
            100                 105                 110         


Ser Glu Ser His Ser His Trp Val His Ser Pro Ile Glu Cys Thr Glu 
        115                 120                 125             


Leu Asp Leu Gln Lys Phe Ser Ser Ser Ala Ser Tyr Thr Gly Ala Glu 
    130                 135                 140                 


Thr Leu Gly Gly Asn Thr Val Glu Ser Thr Gly Asp Arg Leu Glu Leu 
145                 150                 155                 160 


Asn Val Pro Phe Glu Val Pro Ser Pro 
                165                 


<210>  4
<211>  62
<212>  PRT
<213>  Homo sapiens

<400>  4

Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln 
1               5                   10                  15      


Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr 
            20                  25                  30          


Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln 
        35                  40                  45              


Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln 
    50                  55                  60          


<210>  5
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  5

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  6
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  6

Arg Lys Ile Asn Ser Glu Pro Leu Lys Asp Glu Phe Lys Asn Thr Gly 
1               5                   10                  15      


Ser Gly Leu Leu Gly Met Ala Asn Ile Asp Leu Glu Lys Ser Arg Thr 
            20                  25                  30          


Gly Asp Glu Ile Ile Leu Pro Arg Gly Leu Glu Tyr Thr Val Glu Glu 
        35                  40                  45              


Cys Thr Cys Glu Asp Cys Ile Lys Ser Lys Pro Lys Val Asp Ser Asp 
    50                  55                  60                  


His Cys Phe Pro Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val 
65                  70                  75                  80  


Thr Thr Lys Thr Asn Asp Tyr Cys Lys Ser Leu Pro Ala Ala Leu Ser 
                85                  90                  95      


Ala Thr Glu Ile Glu Lys Ser Ile Ser Ala Arg 
            100                 105         


<210>  7
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  7

Arg Arg Cys Arg Arg Arg Glu Lys Phe Thr Thr Pro Ile Glu Glu Thr 
1               5                   10                  15      


Gly Gly Glu Gly Cys Pro Ala Val Ala Leu Ile Gln 
            20                  25              


<210>  8
<211>  154
<212>  PRT
<213>  Homo sapiens

<400>  8

Arg Lys Lys Phe Ile Ser Tyr Leu Lys Gly Ile Cys Ser Gly Gly Gly 
1               5                   10                  15      


Gly Gly Pro Glu Arg Val His Arg Val Leu Phe Arg Arg Arg Ser Cys 
            20                  25                  30          


Pro Ser Arg Val Pro Gly Ala Glu Asp Asn Ala Arg Asn Glu Thr Leu 
        35                  40                  45              


Ser Asn Arg Tyr Leu Gln Pro Thr Gln Val Ser Glu Gln Glu Ile Gln 
    50                  55                  60                  


Gly Gln Glu Leu Ala Glu Leu Thr Gly Val Thr Val Glu Ser Pro Glu 
65                  70                  75                  80  


Glu Pro Gln Arg Leu Leu Glu Gln Ala Glu Ala Glu Gly Cys Gln Arg 
                85                  90                  95      


Arg Arg Leu Leu Val Pro Val Asn Asp Ala Asp Ser Ala Asp Ile Ser 
            100                 105                 110         


Thr Leu Leu Asp Ala Ser Ala Thr Leu Glu Glu Gly His Ala Lys Glu 
        115                 120                 125             


Thr Ile Gln Asp Gln Leu Val Gly Ser Glu Lys Leu Phe Tyr Glu Glu 
    130                 135                 140                 


Asp Glu Ala Gly Ser Ala Thr Ser Cys Leu 
145                 150                 


<210>  9
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR DcR3 domain sequence

<400>  9

Val Ala Glu Thr Pro Thr Tyr Pro Trp Arg Asp Ala Glu Thr Gly Glu 
1               5                   10                  15      


Arg Leu Val Cys Ala Gln Cys Pro Pro Gly Thr Phe Val Gln Arg Pro 
            20                  25                  30          


Cys Arg Arg Asp Ser Pro Thr Thr Cys Gly Pro Cys Pro Pro Arg His 
        35                  40                  45              


Tyr Thr Gln Phe Trp Asn Tyr Leu Glu Arg Cys Arg Tyr Cys Asn Val 
    50                  55                  60                  


Leu Cys Gly Glu Arg Glu Glu Glu Ala Arg Ala Cys His Ala Thr His 
65                  70                  75                  80  


Asn Arg Ala Cys Arg Cys Arg Thr Gly Phe Phe Ala His Ala Gly Phe 
                85                  90                  95      


Cys Leu Glu His Ala Ser Cys Pro Pro Gly Ala Gly Val Ile Ala Pro 
            100                 105                 110         


Gly Thr Pro Ser Gln Asn Thr Gln Cys Gln Pro Cys Pro Pro Gly Thr 
        115                 120                 125             


Phe Ser Ala Ser Ser Ser Ser Ser Glu Gln Cys Gln Pro His Arg Asn 
    130                 135                 140                 


Cys Thr Ala Leu Gly Leu Ala Leu Asn Val Pro Gly Ser Ser Ser His 
145                 150                 155                 160 


Asp Thr Leu Cys Thr Ser Cys Thr Gly Phe Pro Leu Ser Thr Arg Val 
                165                 170                 175     


Pro Gly Ala Glu Glu Cys Glu Arg Ala Val Ile Asp Phe Val Ala Phe 
            180                 185                 190         


Gln Asp Ile Ser Ile Lys Arg Leu Gln Arg Leu Leu Gln Ala Leu Glu 
        195                 200                 205             


Ala Pro Glu Gly Trp Gly Pro Thr Pro Arg Ala Gly Arg Ala Ala Leu 
    210                 215                 220                 


Gln Leu Lys Leu Arg Arg Arg Leu Thr Glu Leu Leu Gly Ala Gln Asp 
225                 230                 235                 240 


Gly Ala Leu Leu Val Arg Leu Leu Gln Ala Leu Arg Val Ala Arg Met 
                245                 250                 255     


Pro Gly Leu Glu Arg Ser Val Arg Glu Arg Phe Leu Pro Val His 
            260                 265                 270     


<210>  10
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, mutant DcR3

<400>  10

Val Ala Glu Thr Pro Thr Tyr Pro Trp Arg Asp Ala Glu Thr Gly Glu 
1               5                   10                  15      


Arg Leu Val Cys Ala Gln Cys Pro Pro Gly Thr Phe Val Gln Arg Pro 
            20                  25                  30          


Cys Arg Arg Asp Ser Pro Thr Thr Cys Gly Pro Cys Pro Pro Arg His 
        35                  40                  45              


Tyr Thr Gln Phe Trp Asn Leu Glu Arg Cys Arg Tyr Cys Asn Val Leu 
    50                  55                  60                  


Cys Gly Glu Arg Glu Glu Glu Ala Arg Ala Cys His Ala Thr His Asn 
65                  70                  75                  80  


Arg Ala Cys Arg Cys Arg Thr Gly Phe Phe Ala His Ala Gly Phe Cys 
                85                  90                  95      


Leu Glu His Ala Ser Cys Pro Pro Gly Ala Gly Val Ile Ala Pro Gly 
            100                 105                 110         


Thr Pro Ser Gln Asn Thr Gln Cys Gln Pro Cys Pro Pro Gly Thr Phe 
        115                 120                 125             


Ser Ala Ser Ser Ser Ser Ser Glu Gln Cys Gln Pro His Arg Asn Cys 
    130                 135                 140                 


Thr Ala Leu Gly Leu Ala Leu Asn Val Pro Gly Ser Ser Ser His Asp 
145                 150                 155                 160 


Thr Leu Cys Thr Ser Cys Thr Gly Phe Pro Leu Ser Thr Arg Val Pro 
                165                 170                 175     


Gly Ala Glu Glu Cys Glu Arg Ala Val Ile Asp Phe Val Ala Phe Gln 
            180                 185                 190         


Asp Ile Ser Ile Lys Arg Leu Gln Arg Leu Leu Gln Ala Leu Glu Ala 
        195                 200                 205             


Pro Glu Gly Trp Gly Pro Thr Pro Arg Ala Gly Arg Ala Ala Leu Gln 
    210                 215                 220                 


Leu Ala Leu Ala Ala Arg Leu Thr Glu Leu Leu Gly Ala Gln Asp Gly 
225                 230                 235                 240 


Ala Leu Leu Val Arg Leu Leu Gln Ala Leu Arg Val Ala Arg Met Pro 
                245                 250                 255     


Gly Leu Glu Arg Ser Val Arg Glu Arg Phe Leu Pro Val His 
            260                 265                 270 


<210>  11
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD8stk

<400>  11

Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 
1               5                   10                  15      


Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 
            20                  25                  30          


Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45      


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, Hinge

<400>  12

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  13
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, COMP

<400>  13

Ala Gly Ser Asp Leu Gly Pro Gln Met Leu Arg Glu Leu Gln Glu Thr 
1               5                   10                  15      


Asn Ala Ala Leu Gln Asp Val Arg Glu Leu Leu Arg Gln Gln Val Arg 
            20                  25                  30          


Glu Ile Thr Phe Leu Lys Asn Thr Val Met Glu Cys Asp Ala Cys Gly 
        35                  40                  45              


Ser Gly Lys 
    50      


<210>  14
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CH1-Hinge

<400>  14

Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
1               5                   10                  15      


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
            20                  25                  30          


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
        35                  40                  45              


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
    50                  55                  60                  


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
65                  70                  75                  80  


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu 
                85                  90                  95      


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
            100                 105                 110 


<210>  15
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CH1-Hinge-CH2-CH3

<400>  15

Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr 
1               5                   10                  15      


Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
            20                  25                  30          


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
        35                  40                  45              


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
    50                  55                  60                  


Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile 
65                  70                  75                  80  


Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val 
                85                  90                  95      


Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
            100                 105                 110         


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
        195                 200                 205             


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Pro Gly Lys 
                325         


<210>  16
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, HuIG Kappa

<400>  16

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  17
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, CD28 Stalk

<400>  17

Ala Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys 
1               5                   10                  15      


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
            20                  25                  30          


Pro Leu Phe Pro Gly Pro Ser Lys Pro 
        35                  40      


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  polar anchor sequence

<400>  18

Arg Lys Lys Arg 
1               


<210>  19
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rigid linker with truncations

<400>  19

Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
1               5                   10                  15      


Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys 
            20                  25                  30          


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45              


Leu Glu 
    50  


<210>  20
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  20

Leu Gly Trp Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp 
1               5                   10                  15      


Val 
    


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DcR2 transmembrane domain

<400>  21

Tyr Leu Ile Ile Ile Val Val Leu Val Ile Ile Leu Ala Val Val Val 
1               5                   10                  15      


Val Gly Phe Ser Cys 
            20      


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GITR transmembrane domain

<400>  22

Leu Gly Trp Leu Thr Val Val Leu Leu Ala Val Ala Ala Cys Val Leu 
1               5                   10                  15      


Leu Leu Thr Ser Ala 
            20      


<210>  23
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD30 transmembrane domain

<400>  23

Pro Val Leu Phe Trp Val Ile Leu Val Leu Val Val Val Val Gly Ser 
1               5                   10                  15      


Ser Ala Phe Leu Leu 
            20      


<210>  24
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  24

Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
1               5                   10                  15      


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
            20                  25                  30          


Cys Asp 
        


<210>  25
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  25

Phe Leu Phe Val Leu Leu Gly Val Gly Ser Met Gly Val Ala Ala Ile 
1               5                   10                  15      


Val Trp Gly Ala Trp 
            20      


<210>  26
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB transmembrane domain

<400>  26

Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  27
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DR3 transmembrane domain

<400>  27

Met Phe Trp Val Gln Val Leu Leu Ala Gly Leu Val Val Pro Leu Leu 
1               5                   10                  15      


Leu Gly Ala Thr Leu 
            20      


<210>  28
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 transmembrane domain

<400>  28

Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro 
1               5                   10                  15      


Leu Ala Ile Leu Leu 
            20      


<210>  29
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD70 transmembrane domain

<400>  29

Val Leu Arg Ala Ala Leu Val Pro Leu Val Ala Gly Leu Val Ile Cys 
1               5                   10                  15      


Leu Val Val Cys Ile 
            20      


<210>  30
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40 transmembrane domain

<400>  30

Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu 
1               5                   10                  15      


Leu Val Leu Val Phe Ile 
            20          


<210>  31
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XEDAR transmembrane domain

<400>  31

Leu Val Ala Leu Val Ser Ser Leu Leu Val Val Phe Thr Leu Ala Phe 
1               5                   10                  15      


Leu Gly Leu Phe Phe 
            20      


<210>  32
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fn14 transmembrane domain

<400>  32

Ile Leu Gly Gly Ala Leu Ser Leu Thr Phe Val Leu Gly Leu Leu Ser 
1               5                   10                  15      


Gly Phe Leu Val Trp 
            20      


<210>  33
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BCMA transmembrane domain

<400>  33

Ile Leu Trp Thr Cys Leu Gly Leu Ser Leu Ile Ile Ser Leu Ala Val 
1               5                   10                  15      


Phe Val Leu Met Phe Leu Leu 
            20              


<210>  34
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD27 transmembrane domain

<400>  34

Ile Leu Val Ile Phe Ser Gly Met Phe Leu Val Phe Thr Leu Ala Gly 
1               5                   10                  15      


Ala Leu Phe Leu His 
            20      


<210>  35
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FasL-binding receptor (FLBR) sequence, human Fas-Dcr2

<400>  35

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Arg Lys Lys Phe Ile Ser Tyr Leu 
            180                 185                 190         


Lys Gly Ile Cys Ser Gly Gly Gly Gly Gly Pro Glu Arg Val His Arg 
        195                 200                 205             


Val Leu Phe Arg Arg Arg Ser Cys Pro Ser Arg Val Pro Gly Ala Glu 
    210                 215                 220                 


Asp Asn Ala Arg Asn Glu Thr Leu Ser Asn Arg Tyr Leu Gln Pro Thr 
225                 230                 235                 240 


Gln Val Ser Glu Gln Glu Ile Gln Gly Gln Glu Leu Ala Glu Leu Thr 
                245                 250                 255     


Gly Val Thr Val Glu Ser Pro Glu Glu Pro Gln Arg Leu Leu Glu Gln 
            260                 265                 270         


Ala Glu Ala Glu Gly Cys Gln Arg Arg Arg Leu Leu Val Pro Val Asn 
        275                 280                 285             


Asp Ala Asp Ser Ala Asp Ile Ser Thr Leu Leu Asp Ala Ser Ala Thr 
    290                 295                 300                 


Leu Glu Glu Gly His Ala Lys Glu Thr Ile Gln Asp Gln Leu Val Gly 
305                 310                 315                 320 


Ser Glu Lys Leu Phe Tyr Glu Glu Asp Glu Ala Gly Ser Ala Thr Ser 
                325                 330                 335     


Cys Leu 
        


<210>  36
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-GITR

<400>  36

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Arg Lys Gln Leu Gly Leu His Ile 
            180                 185                 190         


Trp Gln Leu Arg Ser Gln Cys Met Trp Pro Arg Glu Thr Gln Leu Leu 
        195                 200                 205             


Leu Glu Val Pro Pro Ser Thr Glu Asp Ala Arg Ser Cys Gln Phe Pro 
    210                 215                 220                 


Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu Glu Lys Gly Arg Leu Gly 
225                 230                 235                 240 


Asp Leu Trp Val 
                


<210>  37
<211>  374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-CD30

<400>  37

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Arg Lys His Arg Arg Ala Cys Arg 
            180                 185                 190         


Lys Arg Ile Arg Gln Lys Leu His Leu Cys Tyr Pro Val Gln Thr Ser 
        195                 200                 205             


Gln Pro Lys Leu Glu Leu Val Asp Ser Arg Pro Arg Arg Ser Ser Thr 
    210                 215                 220                 


Gln Leu Arg Ser Gly Ala Ser Val Thr Glu Pro Val Ala Glu Glu Arg 
225                 230                 235                 240 


Gly Leu Met Ser Gln Pro Leu Met Glu Thr Cys His Ser Val Gly Ala 
                245                 250                 255     


Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro Ala Gly Gly 
            260                 265                 270         


Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro Arg Val Ser Thr Glu His 
        275                 280                 285             


Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala Asp Thr Val 
    290                 295                 300                 


Ile Val Gly Thr Val Lys Ala Glu Leu Pro Glu Gly Arg Gly Leu Ala 
305                 310                 315                 320 


Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala Asp His Thr 
                325                 330                 335     


Pro His Tyr Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly Ser Cys Ser 
            340                 345                 350         


Asp Val Met Leu Ser Val Glu Glu Glu Gly Lys Glu Asp Pro Leu Pro 
        355                 360                 365             


Thr Ala Ala Ser Gly Lys 
    370                 


<210>  38
<211>  322
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-XEDAR

<400>  38

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Leu Tyr Cys Lys Gln Phe Phe Asn 
            180                 185                 190         


Arg His Cys Gln Arg Gly Gly Leu Leu Gln Phe Glu Ala Asp Lys Thr 
        195                 200                 205             


Ala Lys Glu Glu Ser Leu Phe Pro Val Pro Pro Ser Lys Glu Thr Ser 
    210                 215                 220                 


Ala Glu Ser Gln Val Ser Glu Asn Ile Phe Gln Thr Gln Pro Leu Asn 
225                 230                 235                 240 


Pro Ile Leu Glu Asp Asp Cys Ser Ser Thr Ser Gly Phe Pro Thr Gln 
                245                 250                 255     


Glu Ser Phe Thr Met Ala Ser Cys Thr Ser Glu Ser His Ser His Trp 
            260                 265                 270         


Val His Ser Pro Ile Glu Cys Thr Glu Leu Asp Leu Gln Lys Phe Ser 
        275                 280                 285             


Ser Ser Ala Ser Tyr Thr Gly Ala Glu Thr Leu Gly Gly Asn Thr Val 
    290                 295                 300                 


Glu Ser Thr Gly Asp Arg Leu Glu Leu Asn Val Pro Phe Glu Val Pro 
305                 310                 315                 320 


Ser Pro 
        


<210>  39
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-CD27

<400>  39

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Gln Arg Arg Lys Tyr Arg Ser Asn 
            180                 185                 190         


Lys Gly Glu Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys 
        195                 200                 205             


Pro Arg Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg 
    210                 215                 220                 


Lys Pro Glu Pro Ala Cys Ser Pro 
225                 230         


<210>  40
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-BCMA

<400>  40

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Arg Lys Ile Asn Ser Glu Pro Leu 
            180                 185                 190         


Lys Asp Glu Phe Lys Asn Thr Gly Ser Gly Leu Leu Gly Met Ala Asn 
        195                 200                 205             


Ile Asp Leu Glu Lys Ser Arg Thr Gly Asp Glu Ile Ile Leu Pro Arg 
    210                 215                 220                 


Gly Leu Glu Tyr Thr Val Glu Glu Cys Thr Cys Glu Asp Cys Ile Lys 
225                 230                 235                 240 


Ser Lys Pro Lys Val Asp Ser Asp His Cys Phe Pro Leu Pro Ala Met 
                245                 250                 255     


Glu Glu Gly Ala Thr Ile Leu Val Thr Thr Lys Thr Asn Asp Tyr Cys 
            260                 265                 270         


Lys Ser Leu Pro Ala Ala Leu Ser Ala Thr Glu Ile Glu Lys Ser Ile 
        275                 280                 285             


Ser Ala Arg 
    290     


<210>  41
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-CD40

<400>  41

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Lys Lys Val Ala Lys Lys Pro Thr 
            180                 185                 190         


Asn Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro 
        195                 200                 205             


Asp Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu 
    210                 215                 220                 


His Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile 
225                 230                 235                 240 


Ser Val Gln Glu Arg Gln 
                245     


<210>  42
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLBR sequence, human Fas-Fn14

<400>  42

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg 
            20                  25                  30          


Lys Thr Val Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His 
        35                  40                  45              


Asp Gly Gln Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala 
    50                  55                  60                  


Arg Asp Cys Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln 
65                  70                  75                  80  


Glu Gly Lys Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg 
                85                  90                  95      


Arg Cys Arg Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn 
            100                 105                 110         


Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe 
        115                 120                 125             


Cys Asn Ser Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu 
    130                 135                 140                 


His Gly Ile Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys 
145                 150                 155                 160 


Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp Leu Cys Leu Leu Leu Leu 
                165                 170                 175     


Pro Ile Pro Leu Ile Val Trp Val Arg Arg Cys Arg Arg Arg Glu Lys 
            180                 185                 190         


Phe Thr Thr Pro Ile Glu Glu Thr Gly Gly Glu Gly Cys Pro Ala Val 
        195                 200                 205             


Ala Leu Ile Gln 
    210         


<210>  43
<211>  394
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  complete FLBR sequence, human DcR3-CD8 stalk/TM/rigid linker

<400>  43

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Val Ala Glu Thr Pro Thr Tyr Pro Trp Arg Asp Ala 
            20                  25                  30          


Glu Thr Gly Glu Arg Leu Val Cys Ala Gln Cys Pro Pro Gly Thr Phe 
        35                  40                  45              


Val Gln Arg Pro Cys Arg Arg Asp Ser Pro Thr Thr Cys Gly Pro Cys 
    50                  55                  60                  


Pro Pro Arg His Tyr Thr Gln Phe Trp Asn Tyr Leu Glu Arg Cys Arg 
65                  70                  75                  80  


Tyr Cys Asn Val Leu Cys Gly Glu Arg Glu Glu Glu Ala Arg Ala Cys 
                85                  90                  95      


His Ala Thr His Asn Arg Ala Cys Arg Cys Arg Thr Gly Phe Phe Ala 
            100                 105                 110         


His Ala Gly Phe Cys Leu Glu His Ala Ser Cys Pro Pro Gly Ala Gly 
        115                 120                 125             


Val Ile Ala Pro Gly Thr Pro Ser Gln Asn Thr Gln Cys Gln Pro Cys 
    130                 135                 140                 


Pro Pro Gly Thr Phe Ser Ala Ser Ser Ser Ser Ser Glu Gln Cys Gln 
145                 150                 155                 160 


Pro His Arg Asn Cys Thr Ala Leu Gly Leu Ala Leu Asn Val Pro Gly 
                165                 170                 175     


Ser Ser Ser His Asp Thr Leu Cys Thr Ser Cys Thr Gly Phe Pro Leu 
            180                 185                 190         


Ser Thr Arg Val Pro Gly Ala Glu Glu Cys Glu Arg Ala Val Ile Asp 
        195                 200                 205             


Phe Val Ala Phe Gln Asp Ile Ser Ile Lys Arg Leu Gln Arg Leu Leu 
    210                 215                 220                 


Gln Ala Leu Glu Ala Pro Glu Gly Trp Gly Pro Thr Pro Arg Ala Gly 
225                 230                 235                 240 


Arg Ala Ala Leu Gln Leu Lys Leu Arg Arg Arg Leu Thr Glu Leu Leu 
                245                 250                 255     


Gly Ala Gln Asp Gly Ala Leu Leu Val Arg Leu Leu Gln Ala Leu Arg 
            260                 265                 270         


Val Ala Arg Met Pro Gly Leu Glu Arg Ser Val Arg Glu Arg Phe Leu 
        275                 280                 285             


Pro Val His Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
    290                 295                 300                 


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
305                 310                 315                 320 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
                325                 330                 335     


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
            340                 345                 350         


Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Lys Lys Arg Ser 
        355                 360                 365             


Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
    370                 375                 380                 


Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu 
385                 390                 


<210>  44
<211>  394
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  complete FLBR sequence, human mutant DcR3-CD8 stalk/TM/rigid 
       linker

<400>  44

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Val Ala Glu Thr Pro Thr Tyr Pro Trp Arg Asp Ala 
            20                  25                  30          


Glu Thr Gly Glu Arg Leu Val Cys Ala Gln Cys Pro Pro Gly Thr Phe 
        35                  40                  45              


Val Gln Arg Pro Cys Arg Arg Asp Ser Pro Thr Thr Cys Gly Pro Cys 
    50                  55                  60                  


Pro Pro Arg His Tyr Thr Gln Phe Trp Asn Tyr Leu Glu Arg Cys Arg 
65                  70                  75                  80  


Tyr Cys Asn Val Leu Cys Gly Glu Arg Glu Glu Glu Ala Arg Ala Cys 
                85                  90                  95      


His Ala Thr His Asn Arg Ala Cys Arg Cys Arg Thr Gly Phe Phe Ala 
            100                 105                 110         


His Ala Gly Phe Cys Leu Glu His Ala Ser Cys Pro Pro Gly Ala Gly 
        115                 120                 125             


Val Ile Ala Pro Gly Thr Pro Ser Gln Asn Thr Gln Cys Gln Pro Cys 
    130                 135                 140                 


Pro Pro Gly Thr Phe Ser Ala Ser Ser Ser Ser Ser Glu Gln Cys Gln 
145                 150                 155                 160 


Pro His Arg Asn Cys Thr Ala Leu Gly Leu Ala Leu Asn Val Pro Gly 
                165                 170                 175     


Ser Ser Ser His Asp Thr Leu Cys Thr Ser Cys Thr Gly Phe Pro Leu 
            180                 185                 190         


Ser Thr Arg Val Pro Gly Ala Glu Glu Cys Glu Arg Ala Val Ile Asp 
        195                 200                 205             


Phe Val Ala Phe Gln Asp Ile Ser Ile Lys Arg Leu Gln Arg Leu Leu 
    210                 215                 220                 


Gln Ala Leu Glu Ala Pro Glu Gly Trp Gly Pro Thr Pro Arg Ala Gly 
225                 230                 235                 240 


Arg Ala Ala Leu Gln Leu Ala Leu Ala Ala Arg Leu Thr Glu Leu Leu 
                245                 250                 255     


Gly Ala Gln Asp Gly Ala Leu Leu Val Arg Leu Leu Gln Ala Leu Arg 
            260                 265                 270         


Val Ala Arg Met Pro Gly Leu Glu Arg Ser Val Arg Glu Arg Phe Leu 
        275                 280                 285             


Pro Val His Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
    290                 295                 300                 


Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
305                 310                 315                 320 


Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
                325                 330                 335     


Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
            340                 345                 350         


Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg Lys Lys Arg Ser 
        355                 360                 365             


Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
    370                 375                 380                 


Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu 
385                 390                 


<210>  45
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta endodomain

<400>  45

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  46
<211>  368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB and CD3-zeta endodomains

<400>  46

Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu 
1               5                   10                  15      


Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro 
            20                  25                  30          


Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys 
        35                  40                  45              


Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile 
    50                  55                  60                  


Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser 
65                  70                  75                  80  


Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly 
                85                  90                  95      


Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu 
            100                 105                 110         


Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln 
        115                 120                 125             


Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys 
    130                 135                 140                 


Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala 
                165                 170                 175     


Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu 
            180                 185                 190         


Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu 
        195                 200                 205             


Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    210                 215                 220                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
225                 230                 235                 240 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
                245                 250                 255     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            260                 265                 270         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        275                 280                 285             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    290                 295                 300                 


Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
305                 310                 315                 320 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                325                 330                 335     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            340                 345                 350         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 365             


<210>  47
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 and CD3-zeta endodomains

<400>  47

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
        35                  40                  45              


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
65                  70                  75                  80  


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                85                  90                  95      


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            100                 105                 110         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        115                 120                 125             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    130                 135                 140                 


His Met Gln Ala Leu Pro Pro Arg 
145                 150         


<210>  48
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28, OX40 and CD3-zeta endodomains

<400>  48

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp 
        35                  40                  45              


Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu 
    50                  55                  60                  


Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe 
65                  70                  75                  80  


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                85                  90                  95      


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            100                 105                 110         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        115                 120                 125             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    130                 135                 140                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
145                 150                 155                 160 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                165                 170                 175     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185             


<210>  49
<211>  335
<212>  PRT
<213>  Homo sapiens

<400>  49

Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala 
1               5                   10                  15      


Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser 
            20                  25                  30          


Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn 
        35                  40                  45              


Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro 
    50                  55                  60                  


Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro 
65                  70                  75                  80  


Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His 
                85                  90                  95      


Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly 
            100                 105                 110         


Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg 
        115                 120                 125             


Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp 
    130                 135                 140                 


Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr 
145                 150                 155                 160 


Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp 
                165                 170                 175     


Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp Val Lys Arg 
            180                 185                 190         


Lys Glu Val Gln Lys Thr Cys Arg Lys His Arg Lys Glu Asn Gln Gly 
        195                 200                 205             


Ser His Glu Ser Pro Thr Leu Asn Pro Glu Thr Val Ala Ile Asn Leu 
    210                 215                 220                 


Ser Asp Val Asp Leu Ser Lys Tyr Ile Thr Thr Ile Ala Gly Val Met 
225                 230                 235                 240 


Thr Leu Ser Gln Val Lys Gly Phe Val Arg Lys Asn Gly Val Asn Glu 
                245                 250                 255     


Ala Lys Ile Asp Glu Ile Lys Asn Asp Asn Val Gln Asp Thr Ala Glu 
            260                 265                 270         


Gln Lys Val Gln Leu Leu Arg Asn Trp His Gln Leu His Gly Lys Lys 
        275                 280                 285             


Glu Ala Tyr Asp Thr Leu Ile Lys Asp Leu Lys Lys Ala Asn Leu Cys 
    290                 295                 300                 


Thr Leu Ala Glu Lys Ile Gln Thr Ile Ile Leu Lys Asp Ile Thr Ser 
305                 310                 315                 320 


Asp Ser Glu Asn Ser Asn Phe Arg Asn Glu Ile Gln Ser Leu Val 
                325                 330                 335 


<210>  50
<211>  148
<212>  PRT
<213>  Homo sapiens

<400>  50

Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg Lys Thr Val 
1               5                   10                  15      


Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His Asp Gly Gln 
            20                  25                  30          


Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala Arg Asp Cys 
        35                  40                  45              


Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln Glu Gly Lys 
    50                  55                  60                  


Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg Arg Cys Arg 
65                  70                  75                  80  


Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn Cys Thr Arg 
                85                  90                  95      


Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe Cys Asn Ser 
            100                 105                 110         


Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu His Gly Ile 
        115                 120                 125             


Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys Glu Glu Gly 
    130                 135                 140                 


Ser Arg Ser Asn 
145             


<210>  51
<211>  144
<212>  PRT
<213>  Homo sapiens

<400>  51

Lys Arg Lys Glu Val Gln Lys Thr Cys Arg Lys His Arg Lys Glu Asn 
1               5                   10                  15      


Gln Gly Ser His Glu Ser Pro Thr Leu Asn Pro Glu Thr Val Ala Ile 
            20                  25                  30          


Asn Leu Ser Asp Val Asp Leu Ser Lys Tyr Ile Thr Thr Ile Ala Gly 
        35                  40                  45              


Val Met Thr Leu Ser Gln Val Lys Gly Phe Val Arg Lys Asn Gly Val 
    50                  55                  60                  


Asn Glu Ala Lys Ile Asp Glu Ile Lys Asn Asp Asn Val Gln Asp Thr 
65                  70                  75                  80  


Ala Glu Gln Lys Val Gln Leu Leu Arg Asn Trp His Gln Leu His Gly 
                85                  90                  95      


Lys Lys Glu Ala Tyr Asp Thr Leu Ile Lys Asp Leu Lys Lys Ala Asn 
            100                 105                 110         


Leu Cys Thr Leu Ala Glu Lys Ile Gln Thr Ile Ile Leu Lys Asp Ile 
        115                 120                 125             


Thr Asp Ser Glu Asn Ser Asn Phe Arg Asn Glu Ile Gln Ser Leu Val 
    130                 135                 140                 


<210>  52
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain (TM) domain

<400>  52

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  53
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 TM domain

<400>  53

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  54
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  54

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  55
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM (immunoreceptor tyrosine-based activation motif)


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Leu or Ile

<400>  55

Tyr Xaa Xaa Xaa 
1               


