               SEQUENCE LISTING

<110> Bayer AG
      Deutsches Krebsforschungszentrum (DKFZ)

<120> SUBSTITUTED AMINOTHIAZOLES AS DGKZETA INHIBITORS FOR IMMUNE ACTIVATION 

<130> BHC 21 3 007 WO-PCT

<150> EP 20171280.9 
<151> 2020-04-24

<160> 5

<170> BiSSAP 1.3.6

<210> 1
<211> 938
<212> PRT
<213> human DGKzM1


<220> 
<223> DGKz hu 1 encoding human DGKzM1 to V928 plus N-terminal Flag-Tag

<400> 1
Met Gly Asp Tyr Lys Asp Asp Asp Asp Lys Met Glu Pro Arg Asp Gly 
1               5                   10                  15      
Ser Pro Glu Ala Arg Ser Ser Asp Ser Glu Ser Ala Ser Ala Ser Ser 
            20                  25                  30          
Ser Gly Ser Glu Arg Asp Ala Gly Pro Glu Pro Asp Lys Ala Pro Arg 
        35                  40                  45              
Arg Leu Asn Lys Arg Arg Phe Pro Gly Leu Arg Leu Phe Gly His Arg 
    50                  55                  60                  
Lys Ala Ile Thr Lys Ser Gly Leu Gln His Leu Ala Pro Pro Pro Pro 
65                  70                  75                  80  
Thr Pro Gly Ala Pro Cys Ser Glu Ser Glu Arg Gln Ile Arg Ser Thr 
                85                  90                  95      
Val Asp Trp Ser Glu Ser Ala Thr Tyr Gly Glu His Ile Trp Phe Glu 
            100                 105                 110         
Thr Asn Val Ser Gly Asp Phe Cys Tyr Val Gly Glu Gln Tyr Cys Val 
        115                 120                 125             
Ala Arg Met Leu Lys Ser Val Ser Arg Arg Lys Cys Ala Ala Cys Lys 
    130                 135                 140                 
Ile Val Val His Thr Pro Cys Ile Glu Gln Leu Glu Lys Ile Asn Phe 
145                 150                 155                 160 
Arg Cys Lys Pro Ser Phe Arg Glu Ser Gly Ser Arg Asn Val Arg Glu 
                165                 170                 175     
Pro Thr Phe Val Arg His His Trp Val His Arg Arg Arg Gln Asp Gly 
            180                 185                 190         
Lys Cys Arg His Cys Gly Lys Gly Phe Gln Gln Lys Phe Thr Phe His 
        195                 200                 205             
Ser Lys Glu Ile Val Ala Ile Ser Cys Ser Trp Cys Lys Gln Ala Tyr 
    210                 215                 220                 
His Ser Lys Val Ser Cys Phe Met Leu Gln Gln Ile Glu Glu Pro Cys 
225                 230                 235                 240 
Ser Leu Gly Val His Ala Ala Val Val Ile Pro Pro Thr Trp Ile Leu 
                245                 250                 255     
Arg Ala Arg Arg Pro Gln Asn Thr Leu Lys Ala Ser Lys Lys Lys Lys 
            260                 265                 270         
Arg Ala Ser Phe Lys Arg Lys Ser Ser Lys Lys Gly Pro Glu Glu Gly 
        275                 280                 285             
Arg Trp Arg Pro Phe Ile Ile Arg Pro Thr Pro Ser Pro Leu Met Lys 
    290                 295                 300                 
Pro Leu Leu Val Phe Val Asn Pro Lys Ser Gly Gly Asn Gln Gly Ala 
305                 310                 315                 320 
Lys Ile Ile Gln Ser Phe Leu Trp Tyr Leu Asn Pro Arg Gln Val Phe 
                325                 330                 335     
Asp Leu Ser Gln Gly Gly Pro Lys Glu Ala Leu Glu Met Tyr Arg Lys 
            340                 345                 350         
Val His Asn Leu Arg Ile Leu Ala Cys Gly Gly Asp Gly Thr Val Gly 
        355                 360                 365             
Trp Ile Leu Ser Thr Leu Asp Gln Leu Arg Leu Lys Pro Pro Pro Pro 
    370                 375                 380                 
Val Ala Ile Leu Pro Leu Gly Thr Gly Asn Asp Leu Ala Arg Thr Leu 
385                 390                 395                 400 
Asn Trp Gly Gly Gly Tyr Thr Asp Glu Pro Val Ser Lys Ile Leu Ser 
                405                 410                 415     
His Val Glu Glu Gly Asn Val Val Gln Leu Asp Arg Trp Asp Leu His 
            420                 425                 430         
Ala Glu Pro Asn Pro Glu Ala Gly Pro Glu Asp Arg Asp Glu Gly Ala 
        435                 440                 445             
Thr Asp Arg Leu Pro Leu Asp Val Phe Asn Asn Tyr Phe Ser Leu Gly 
    450                 455                 460                 
Phe Asp Ala His Val Thr Leu Glu Phe His Glu Ser Arg Glu Ala Asn 
465                 470                 475                 480 
Pro Glu Lys Phe Asn Ser Arg Phe Arg Asn Lys Met Phe Tyr Ala Gly 
                485                 490                 495     
Thr Ala Phe Ser Asp Phe Leu Met Gly Ser Ser Lys Asp Leu Ala Lys 
            500                 505                 510         
His Ile Arg Val Val Cys Asp Gly Met Asp Leu Thr Pro Lys Ile Gln 
        515                 520                 525             
Asp Leu Lys Pro Gln Cys Val Val Phe Leu Asn Ile Pro Arg Tyr Cys 
    530                 535                 540                 
Ala Gly Thr Met Pro Trp Gly His Pro Gly Glu His His Asp Phe Glu 
545                 550                 555                 560 
Pro Gln Arg His Asp Asp Gly Tyr Leu Glu Val Ile Gly Phe Thr Met 
                565                 570                 575     
Thr Ser Leu Ala Ala Leu Gln Val Gly Gly His Gly Glu Arg Leu Thr 
            580                 585                 590         
Gln Cys Arg Glu Val Val Leu Thr Thr Ser Lys Ala Ile Pro Val Gln 
        595                 600                 605             
Val Asp Gly Glu Pro Cys Lys Leu Ala Ala Ser Arg Ile Arg Ile Ala 
    610                 615                 620                 
Leu Arg Asn Gln Ala Thr Met Val Gln Lys Ala Lys Arg Arg Ser Ala 
625                 630                 635                 640 
Ala Pro Leu His Ser Asp Gln Gln Pro Val Pro Glu Gln Leu Arg Ile 
                645                 650                 655     
Gln Val Ser Arg Val Ser Met His Asp Tyr Glu Ala Leu His Tyr Asp 
            660                 665                 670         
Lys Glu Gln Leu Lys Glu Ala Ser Val Pro Leu Gly Thr Val Val Val 
        675                 680                 685             
Pro Gly Asp Ser Asp Leu Glu Leu Cys Arg Ala His Ile Glu Arg Leu 
    690                 695                 700                 
Gln Gln Glu Pro Asp Gly Ala Gly Ala Lys Ser Pro Thr Cys Gln Lys 
705                 710                 715                 720 
Leu Ser Pro Lys Trp Cys Phe Leu Asp Ala Thr Thr Ala Ser Arg Phe 
                725                 730                 735     
Tyr Arg Ile Asp Arg Ala Gln Glu His Leu Asn Tyr Val Thr Glu Ile 
            740                 745                 750         
Ala Gln Asp Glu Ile Tyr Ile Leu Asp Pro Glu Leu Leu Gly Ala Ser 
        755                 760                 765             
Ala Arg Pro Asp Leu Pro Thr Pro Thr Ser Pro Leu Pro Thr Ser Pro 
    770                 775                 780                 
Cys Ser Pro Thr Pro Arg Ser Leu Gln Gly Asp Ala Ala Pro Pro Gln 
785                 790                 795                 800 
Gly Glu Glu Leu Ile Glu Ala Ala Lys Arg Asn Asp Phe Cys Lys Leu 
                805                 810                 815     
Gln Glu Leu His Arg Ala Gly Gly Asp Leu Met His Arg Asp Glu Gln 
            820                 825                 830         
Ser Arg Thr Leu Leu His His Ala Val Ser Thr Gly Ser Lys Asp Val 
        835                 840                 845             
Val Arg Tyr Leu Leu Asp His Ala Pro Pro Glu Ile Leu Asp Ala Val 
    850                 855                 860                 
Glu Glu Asn Gly Glu Thr Cys Leu His Gln Ala Ala Ala Leu Gly Gln 
865                 870                 875                 880 
Arg Thr Ile Cys His Tyr Ile Val Glu Ala Gly Ala Ser Leu Met Lys 
                885                 890                 895     
Thr Asp Gln Gln Gly Asp Thr Pro Arg Gln Arg Ala Glu Lys Ala Gln 
            900                 905                 910         
Asp Thr Glu Leu Ala Ala Tyr Leu Glu Asn Arg Gln His Tyr Gln Met 
        915                 920                 925             
Ile Gln Arg Glu Asp Gln Glu Thr Ala Val 
    930                 935             

<210> 2
<211> 8
<212> PRT
<213> mouse


<220> 
<223> SIINFEKL

<400> 2
Ser Ile Ile Asn Phe Glu Lys Leu 
1               5               

<210> 3
<211> 30
<212> PRT
<213> chicken


<220> 
<223> Ovalbumin antigen OVA-30

<400> 3
Ser Met Leu Val Leu Leu Pro Asp Glu Val Ser Gly Leu Glu Gln Leu 
1               5                   10                  15      
Glu Ser Ile Ile Asn Phe Glu Lys Leu Thr Glu Trp Thr Ser 
            20                  25                  30  

<210> 4
<211> 8
<212> PRT
<213> synthetic organisms


<220> 
<223> FLAG-Tag

<400> 4
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               

<210> 5
<211> 6
<212> PRT
<213> synthetic organisms


<220> 
<223> Kozak sequence for translation initiation

<400> 5
Gly Cys Cys Ala Cys Cys 
1               5       

