                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
       Charit - Universittsmedizin Berlin
       Universitt Greifswald
 
<120>  Synthetic Streptococcus pneumoniae saccharide conjugates to 
       conserved membrane protein

<130>  GAR-P04242WO

<140>  PCT/EP2021/060448
<141>  2021-04-21

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer SH33


<220>
<221>  SH33
<222>  (1)..(27)
<223>  forward primer SH33

<400>  1
ggatccgaag aagaatctcc cgtagcc                                           27


<210>  2
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer PRP2


<220>
<221>  PRP2
<222>  (1)..(27)
<223>  revese primer PRP2

<400>  2
aagcttatta actgctttct taaggtc                                           27


<210>  3
<211>  2086
<212>  DNA
<213>  Streptococcus pneumoniae


<220>
<221>  M74122
<222>  (1)..(2086)
<223>  sequence ID: M74122 (Streptococcus pneumoniae pneumococcal 
       surface protein A (pspA) gene, complete cds)

<400>  3
aagcttatga tatagaaatt tgtaacaaaa atgtaatata aaacacttga caaatattta       60

cggaggaggc ttatacttaa tataagtata gtctgaaaat gactatcaga aaagaggtaa      120

atttagatga ataagaaaaa aatgatttta acaagtctag ccagcgtcgc tatcttaggg      180

gctggttttg ttgcgtctca gcctactgtt gtaagagcag aagaatctcc cgtagccagt      240

cagtctaaag ctgagaaaga ctatgatgca gcgaagaaag atgctaagaa tgcgaaaaaa      300

gcagtagaag atgctcaaaa ggctttagat gatgcaaaag ctgctcagaa aaaatatgac      360

gaggatcaga agaaaactga ggagaaagcc gcgctagaaa aagcagcgtc tgaagagatg      420

gataaggcag tggcagcagt tcaacaagcg tatctagcct atcaacaagc tacagacaaa      480

gccgcaaaag acgcagcaga taagatgata gatgaagcta agaaacgcga agaagaggca      540

aaaactaaat ttaatactgt tcgagcaatg gtagttcctg agccagagca gttggctgag      600

actaagaaaa aatcagaaga agctaaacaa aaagcaccag aacttactaa aaaactagaa      660

gaagctaaag caaaattaga agaggctgag aaaaaagcta ctgaagccaa acaaaaagtg      720

gatgctgaag aagtcgctcc tcaagctaaa atcgctgaat tggaaaatca agttcataga      780

ctagaacaag agctcaaaga gattgatgag tctgaatcag aagattatgc taaagaaggt      840

ttccgtgctc ctcttcaatc taaattggat gccaaaaaag ctaaactatc aaaacttgaa      900

gagttaagtg ataagattga tgagttagac gctgaaattg caaaacttga agatcaactt      960

aaagctgctg aagaaaacaa taatgtagaa gactacttta aagaaggttt agagaaaact     1020

attgctgcta aaaaagctga attagaaaaa actgaagctg accttaagaa agcagttaat     1080

gagccagaaa aaccagctcc agctccagaa actccagccc cagaagcacc agctgaacaa     1140

ccaaaaccag cgccggctcc tcaaccagct cccgcaccaa aaccagagaa gccagctgaa     1200

caaccaaaac cagaaaaaac agatgatcaa caagctgaag aagactatgc tcgtagatca     1260

gaagaagaat ataatcgctt gactcaacag caaccgccaa aagctgaaaa accagctcct     1320

gcaccaaaaa caggctggaa acaagaaaac ggtatgtggt acttctacaa tactgatggt     1380

tcaatggcga caggatggct ccaaaacaac ggttcatggt actacctcaa cagcaatggt     1440

gctatggcta caggttggct ccaatacaat ggttcatggt attacctcaa cgctaacggc     1500

gctatggcaa caggttgggc taaagtcaac ggttcatggt actacctcaa cgctaatggt     1560

gctatggcta caggttggct ccaatacaac ggttcatggt attacctcaa cgctaacggc     1620

gctatggcaa caggttgggc taaagtcaac ggttcatggt actacctcaa cgctaatggt     1680

gctatggcta caggttggct ccaatacaac ggttcatggt actacctcaa cgctaacggt     1740

gctatggcta caggttgggc taaagtcaac ggttcatggt actacctcaa cgctaatggt     1800

gctatggcaa caggttgggt gaaagatgga gatacctggt actatcttga agcatcaggt     1860

gctatgaaag caagccaatg gttcaaagta tcagataaat ggtactatgt caatggttta     1920

ggtgcccttg cagtcaacac aactgtagat ggctataaag tcaatgccaa tggtgaatgg     1980

gtttaagccg attaaattaa agcatgttaa gaacatttga cattttaatt ttgaaacaaa     2040

gataaggttc gattgaatag atttatgttc gtattcttta ggtacc                    2086


<210>  4
<211>  619
<212>  PRT
<213>  Streptococcus pneumoniae


<220>
<221>  sequene AAA27018.1
<222>  (1)..(619)
<223>  pneumococcal surface protein A -sequene AAA27018.1

<400>  4

Met Asn Lys Lys Lys Met Ile Leu Thr Ser Leu Ala Ser Val Ala Ile 
1               5                   10                  15      


Leu Gly Ala Gly Phe Val Ala Ser Gln Pro Thr Val Val Arg Ala Glu 
            20                  25                  30          


Glu Ser Pro Val Ala Ser Gln Ser Lys Ala Glu Lys Asp Tyr Asp Ala 
        35                  40                  45              


Ala Lys Lys Asp Ala Lys Asn Ala Lys Lys Ala Val Glu Asp Ala Gln 
    50                  55                  60                  


Lys Ala Leu Asp Asp Ala Lys Ala Ala Gln Lys Lys Tyr Asp Glu Asp 
65                  70                  75                  80  


Gln Lys Lys Thr Glu Glu Lys Ala Ala Leu Glu Lys Ala Ala Ser Glu 
                85                  90                  95      


Glu Met Asp Lys Ala Val Ala Ala Val Gln Gln Ala Tyr Leu Ala Tyr 
            100                 105                 110         


Gln Gln Ala Thr Asp Lys Ala Ala Lys Asp Ala Ala Asp Lys Met Ile 
        115                 120                 125             


Asp Glu Ala Lys Lys Arg Glu Glu Glu Ala Lys Thr Lys Phe Asn Thr 
    130                 135                 140                 


Val Arg Ala Met Val Val Pro Glu Pro Glu Gln Leu Ala Glu Thr Lys 
145                 150                 155                 160 


Lys Lys Ser Glu Glu Ala Lys Gln Lys Ala Pro Glu Leu Thr Lys Lys 
                165                 170                 175     


Leu Glu Glu Ala Lys Ala Lys Leu Glu Glu Ala Glu Lys Lys Ala Thr 
            180                 185                 190         


Glu Ala Lys Gln Lys Val Asp Ala Glu Glu Val Ala Pro Gln Ala Lys 
        195                 200                 205             


Ile Ala Glu Leu Glu Asn Gln Val His Arg Leu Glu Gln Glu Leu Lys 
    210                 215                 220                 


Glu Ile Asp Glu Ser Glu Ser Glu Asp Tyr Ala Lys Glu Gly Phe Arg 
225                 230                 235                 240 


Ala Pro Leu Gln Ser Lys Leu Asp Ala Lys Lys Ala Lys Leu Ser Lys 
                245                 250                 255     


Leu Glu Glu Leu Ser Asp Lys Ile Asp Glu Leu Asp Ala Glu Ile Ala 
            260                 265                 270         


Lys Leu Glu Asp Gln Leu Lys Ala Ala Glu Glu Asn Asn Asn Val Glu 
        275                 280                 285             


Asp Tyr Phe Lys Glu Gly Leu Glu Lys Thr Ile Ala Ala Lys Lys Ala 
    290                 295                 300                 


Glu Leu Glu Lys Thr Glu Ala Asp Leu Lys Lys Ala Val Asn Glu Pro 
305                 310                 315                 320 


Glu Lys Pro Ala Pro Ala Pro Glu Thr Pro Ala Pro Glu Ala Pro Ala 
                325                 330                 335     


Glu Gln Pro Lys Pro Ala Pro Ala Pro Gln Pro Ala Pro Ala Pro Lys 
            340                 345                 350         


Pro Glu Lys Pro Ala Glu Gln Pro Lys Pro Glu Lys Thr Asp Asp Gln 
        355                 360                 365             


Gln Ala Glu Glu Asp Tyr Ala Arg Arg Ser Glu Glu Glu Tyr Asn Arg 
    370                 375                 380                 


Leu Thr Gln Gln Gln Pro Pro Lys Ala Glu Lys Pro Ala Pro Ala Pro 
385                 390                 395                 400 


Lys Thr Gly Trp Lys Gln Glu Asn Gly Met Trp Tyr Phe Tyr Asn Thr 
                405                 410                 415     


Asp Gly Ser Met Ala Thr Gly Trp Leu Gln Asn Asn Gly Ser Trp Tyr 
            420                 425                 430         


Tyr Leu Asn Ser Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Tyr Asn 
        435                 440                 445             


Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ala Met Ala Thr Gly Trp 
    450                 455                 460                 


Ala Lys Val Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ala Met 
465                 470                 475                 480 


Ala Thr Gly Trp Leu Gln Tyr Asn Gly Ser Trp Tyr Tyr Leu Asn Ala 
                485                 490                 495     


Asn Gly Ala Met Ala Thr Gly Trp Ala Lys Val Asn Gly Ser Trp Tyr 
            500                 505                 510         


Tyr Leu Asn Ala Asn Gly Ala Met Ala Thr Gly Trp Leu Gln Tyr Asn 
        515                 520                 525             


Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ala Met Ala Thr Gly Trp 
    530                 535                 540                 


Ala Lys Val Asn Gly Ser Trp Tyr Tyr Leu Asn Ala Asn Gly Ala Met 
545                 550                 555                 560 


Ala Thr Gly Trp Val Lys Asp Gly Asp Thr Trp Tyr Tyr Leu Glu Ala 
                565                 570                 575     


Ser Gly Ala Met Lys Ala Ser Gln Trp Phe Lys Val Ser Asp Lys Trp 
            580                 585                 590         


Tyr Tyr Val Asn Gly Leu Gly Ala Leu Ala Val Asn Thr Thr Val Asp 
        595                 600                 605             


Gly Tyr Lys Val Asn Ala Asn Gly Glu Trp Val 
    610                 615                 


