                         SEQUENCE LISTING

<110>  Okogen, LLC
       Strem, Brian M.
 
<120>  Ranpirnase for Viral Conjunctivitis

<130>  OKGEN.006WO

<150>  US 63/014,380
<151>  2020-04-23

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  127
<212>  PRT
<213>  Rana pipiens

<400>  1

Met Phe Pro Lys Phe Ser Phe Leu Leu Ile Phe Ala Val Val Leu Ser 
1               5                   10                  15      


Leu Thr His Lys Ser Leu Cys Gln Asp Trp Leu Thr Phe Gln Lys Lys 
            20                  25                  30          


His Ile Thr Asn Thr Arg Asp Val Asp Cys Asp Asn Ile Met Ser Thr 
        35                  40                  45              


Asn Leu Phe His Cys Lys Asp Lys Asn Thr Phe Ile Tyr Ser Arg Pro 
    50                  55                  60                  


Glu Pro Val Lys Ala Ile Cys Lys Gly Ile Ile Ala Ser Lys Asn Val 
65                  70                  75                  80  


Leu Thr Thr Ser Glu Phe Tyr Leu Ser Asp Cys Asn Val Thr Ser Arg 
                85                  90                  95      


Pro Cys Lys Tyr Lys Leu Lys Lys Ser Thr Asn Lys Phe Cys Val Thr 
            100                 105                 110         


Cys Glu Asn Gln Ala Pro Val His Phe Val Gly Val Gly Ser Cys 
        115                 120                 125         


<210>  2
<211>  104
<212>  PRT
<213>  Rana pipiens

<400>  2

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  3
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  3

Glu Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  4
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ranpirnase variant

<400>  4

Glu Asp Trp Leu Thr Phe Gln Lys Lys His Val Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asn Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Arg Cys 
            100                 


<210>  5
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  5

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Cys Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  6
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  6

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Cys Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  7
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  7

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Ala Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Ala 
            100                 


<210>  8
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  8

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Ala Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Ala 
            100                 


<210>  9
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  9

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Asn Cys Ala Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  10
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  10

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Asn Cys Ala Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  11
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  11

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Ala Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Ala Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  12
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  12

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Ala Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Ala Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Cys Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  13
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  13

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Leu Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Ser Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly 
            100         


<210>  14
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Ranpirnase variant

<400>  14

Gln Asp Trp Leu Thr Phe Gln Lys Lys His Ile Thr Asn Thr Arg Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Ile Met Ser Thr Asn Leu Phe His Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Arg Pro Glu Pro Val Lys Ala Ile Cys 
        35                  40                  45              


Lys Gly Ile Ile Ala Ser Lys Asn Val Leu Thr Thr Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Val Thr Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Thr Asn Lys Phe Ser Val Thr Cys Glu Asn Gln Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly 
            100         


<210>  15
<211>  117
<212>  PRT
<213>  Rana dybowskii

<400>  15

Leu Phe Ala Val Val Leu Ser Leu Thr His Lys Phe Leu Cys Gln Asp 
1               5                   10                  15      


Trp Lys Thr Phe Gln Asp Lys His Leu Thr Lys Thr Arg Asp Val Asp 
            20                  25                  30          


Cys Asp Asn Val Leu Ser Lys Pro Leu Phe Asn Cys Lys Asp Arg Asn 
        35                  40                  45              


Thr Phe Ile Phe Ser Arg Pro Glu Pro Val Lys Ala Leu Cys Lys Gly 
    50                  55                  60                  


Val Lys Asp Lys Asn Val Leu Ser Arg Ser Glu Phe Tyr Leu Ser Asp 
65                  70                  75                  80  


Cys Asn Val Thr Thr Arg His Cys Lys Tyr Lys Leu Lys Lys Lys Ile 
                85                  90                  95      


Asn Thr Ile Cys Ile Thr Cys Arg Gly Glu Ala Pro Val His Phe Val 
            100                 105                 110         


Gly Val Gly Ser Cys 
        115         


<210>  16
<211>  104
<212>  PRT
<213>  Rana dybowskii

<400>  16

Gln Asp Trp Lys Thr Phe Gln Lys Lys His Leu Thr Lys Ala Arg Asp 
1               5                   10                  15      


Ile Lys Cys Asp Asn Ile Met Ser Lys Thr Leu Phe Asn Cys Lys Asp 
            20                  25                  30          


Thr Asn Thr Phe Ile Phe Ser Leu Pro Gly Pro Val Lys Ala Leu Cys 
        35                  40                  45              


Arg Gly Ile Lys Val Ser Lys Asn Val Leu Ser Arg Ser Glu Phe Asp 
    50                  55                  60                  


Leu Ser Glu Cys Asn Val Lys Ser Lys Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Lys Ser Asp Gly Ile Cys Ile Thr Cys Arg Asp Glu Ala Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  17
<211>  104
<212>  PRT
<213>  Rana dybowskii

<400>  17

Gln Asp Trp Lys Thr Phe Gln Lys Lys His Leu Thr Asn Thr Arg Asn 
1               5                   10                  15      


Ile Lys Cys Asp Lys Ile Met Pro Ile Asn Leu Phe His Cys Lys Tyr 
            20                  25                  30          


Arg Asn Thr Phe Ile Tyr Ser Arg Pro Arg Gly Val Glu Asn Leu Cys 
        35                  40                  45              


Arg Gly Lys Ile Asn Ala Thr Asn Val Ser Ser Ser Ser Lys Phe Ala 
    50                  55                  60                  


Leu Phe Glu Cys Ile Glu Lys Ser Arg Pro Cys Lys Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Gln Thr Asn Val Ile Cys Ile Thr Cys Glu His Lys Val Pro Val 
                85                  90                  95      


His Phe Val Gly Val Gly Ser Cys 
            100                 


<210>  18
<211>  129
<212>  PRT
<213>  Rana catesbeiana

<400>  18

Met Phe Pro Lys Phe Ser Phe Leu Leu Ile Phe Ala Ile Val Leu Ser 
1               5                   10                  15      


Leu Thr His Lys Ser Leu Cys Gln Asp Trp Ala Thr Phe Lys Lys Lys 
            20                  25                  30          


His Leu Thr Asp Thr Trp Asp Val Asp Cys Asp Asn Leu Met Pro Thr 
        35                  40                  45              


Ser Leu Phe Asp Cys Lys Asp Lys Asn Thr Phe Ile Tyr Ser Leu Pro 
    50                  55                  60                  


Gly Pro Val Lys Ala Leu Cys Arg Gly Val Ile Phe Ser Ala Asp Val 
65                  70                  75                  80  


Leu Ser Asn Ser Glu Phe Tyr Leu Ala Glu Cys Asn Val Lys Pro Arg 
                85                  90                  95      


Lys Pro Cys Lys Tyr Lys Leu Lys Lys Ser Ser Asn Arg Ile Cys Ile 
            100                 105                 110         


Arg Cys Glu His Glu Leu Pro Val His Phe Ala Gly Val Gly Ile Cys 
        115                 120                 125             


Pro 
    


<210>  19
<211>  106
<212>  PRT
<213>  Rana catesbeiana

<400>  19

Gln Asp Trp Ala Thr Phe Lys Lys Lys His Leu Thr Asp Thr Trp Asp 
1               5                   10                  15      


Val Asp Cys Asp Asn Leu Met Pro Thr Ser Leu Phe Asp Cys Lys Asp 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ser Leu Pro Gly Pro Val Lys Ala Leu Cys 
        35                  40                  45              


Arg Gly Val Ile Phe Ser Ala Asp Val Leu Ser Asn Ser Glu Phe Tyr 
    50                  55                  60                  


Leu Ala Glu Cys Asn Val Lys Pro Arg Lys Pro Cys Lys Tyr Lys Leu 
65                  70                  75                  80  


Lys Lys Ser Ser Asn Arg Ile Cys Ile Arg Cys Glu His Glu Leu Pro 
                85                  90                  95      


Val His Phe Ala Gly Val Gly Ile Cys Pro 
            100                 105     


<210>  20
<211>  128
<212>  PRT
<213>  Rana catesbeiana

<400>  20

Met Phe Pro Lys Phe Ser Phe Leu Leu Ile Phe Ala Val Val Leu Ser 
1               5                   10                  15      


Leu Thr His Lys Ser Leu Cys Gln Asn Trp Glu Thr Phe Gln Lys Lys 
            20                  25                  30          


His Leu Thr Asn Ile Leu Asp Ile Asn Cys Asp Val Glu Met Ala Lys 
        35                  40                  45              


Ala Leu Phe Asn Cys Lys Glu Met Asn Thr Phe Ile Tyr Ala Leu Pro 
    50                  55                  60                  


Gly Arg Val Gln Ala Leu Cys Lys Asn Ile Lys Asp Asn Thr Glu Val 
65                  70                  75                  80  


Leu Ser Thr Asp Thr Phe Tyr Leu Pro Glu Cys Asn Arg Ile Lys Leu 
                85                  90                  95      


Pro Cys His Tyr Lys Leu Lys Lys Pro Leu Glu Lys Ile Cys Ile Thr 
            100                 105                 110         


Cys Val Asn Glu Leu Pro Ile His Phe Ala Gly Val Gly Ser Cys Pro 
        115                 120                 125             


<210>  21
<211>  105
<212>  PRT
<213>  Rana catesbeiana

<400>  21

Gln Asn Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asn Ile Leu Asp 
1               5                   10                  15      


Ile Asn Cys Asp Val Glu Met Ala Lys Ala Leu Phe Asn Cys Lys Glu 
            20                  25                  30          


Met Asn Thr Phe Ile Tyr Ala Leu Pro Gly Arg Val Gln Ala Leu Cys 
        35                  40                  45              


Lys Asn Ile Lys Asp Asn Thr Glu Val Leu Ser Thr Asp Thr Phe Tyr 
    50                  55                  60                  


Leu Pro Glu Cys Asn Arg Ile Lys Leu Pro Cys His Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Pro Leu Glu Lys Ile Cys Ile Thr Cys Val Asn Glu Leu Pro Ile 
                85                  90                  95      


His Phe Ala Gly Val Gly Ser Cys Pro 
            100                 105 


<210>  22
<211>  128
<212>  PRT
<213>  Rana catesbeiana

<400>  22

Met Phe Pro Lys Phe Ser Phe Leu Leu Ile Phe Ala Val Val Leu Ser 
1               5                   10                  15      


Leu Thr His Lys Ser Leu Cys Gln Asn Trp Glu Thr Phe Gln Lys Lys 
            20                  25                  30          


His Leu Thr Asn Ile Leu Asp Ile Asn Cys Asp Val Glu Met Ala Lys 
        35                  40                  45              


Ala Leu Phe Asn Cys Lys Glu Met Asn Thr Phe Ile Tyr Ala Leu Pro 
    50                  55                  60                  


Gly Arg Val Gln Ala Leu Cys Lys Asn Ile Arg Asp Asn Thr Asp Val 
65                  70                  75                  80  


Leu Ser Arg Asp Ala Phe Leu Leu Pro Gln Cys Asp Arg Ile Lys Leu 
                85                  90                  95      


Pro Cys His Tyr Lys Leu Ser Ser Ser Thr Asn Thr Ile Cys Ile Thr 
            100                 105                 110         


Cys Val Asn Gln Leu Pro Ile His Phe Ala Gly Val Gly Ser Cys Pro 
        115                 120                 125             


<210>  23
<211>  105
<212>  PRT
<213>  Rana catesbeiana

<400>  23

Gln Asn Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asn Ile Leu Asp 
1               5                   10                  15      


Ile Asn Cys Asp Val Glu Met Ala Lys Ala Leu Phe Asn Cys Lys Glu 
            20                  25                  30          


Met Asn Thr Phe Ile Tyr Ala Leu Pro Gly Arg Val Gln Ala Leu Cys 
        35                  40                  45              


Lys Asn Ile Arg Asp Asn Thr Asp Val Leu Ser Arg Asp Ala Phe Leu 
    50                  55                  60                  


Leu Pro Gln Cys Asp Arg Ile Lys Leu Pro Cys His Tyr Lys Leu Ser 
65                  70                  75                  80  


Ser Ser Thr Asn Thr Ile Cys Ile Thr Cys Val Asn Gln Leu Pro Ile 
                85                  90                  95      


His Phe Ala Gly Val Gly Ser Cys Pro 
            100                 105 


<210>  24
<211>  131
<212>  PRT
<213>  Rana catesbeiana

<400>  24

Met Phe Pro Lys Phe Ser Phe Leu Leu Ile Phe Ala Val Val Leu Ser 
1               5                   10                  15      


Leu Thr His Lys Ser Leu Cys Gln Asp Trp Glu Thr Phe Gln Lys Lys 
            20                  25                  30          


His Leu Thr Asp Thr Val Asp Val Asn Cys Asp Val Glu Met Gln Lys 
        35                  40                  45              


Ala Leu Phe Asn Cys Lys Gln Thr Asn Thr Phe Ile Phe Ala Arg Pro 
    50                  55                  60                  


Pro Arg Val Gln Ala Leu Cys Lys Asn Ile Lys Asp Asn Thr Asp Val 
65                  70                  75                  80  


Leu Ser Arg Asp Glu Phe Tyr Leu Pro Glu Cys Asn Arg Thr Lys Leu 
                85                  90                  95      


Pro Cys His Tyr Lys Leu Lys Lys Pro Leu Asn Thr Ile Cys Leu Thr 
            100                 105                 110         


Cys Arg Lys Glu Leu Pro Val His Phe Ala Gly Val Gly Lys Cys Pro 
        115                 120                 125             


Glu Lys Val 
    130     


<210>  25
<211>  108
<212>  PRT
<213>  Rana catesbeiana

<400>  25

Gln Asp Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Val Asp 
1               5                   10                  15      


Val Asn Cys Asp Val Glu Met Gln Lys Ala Leu Phe Asn Cys Lys Gln 
            20                  25                  30          


Thr Asn Thr Phe Ile Phe Ala Arg Pro Pro Arg Val Gln Ala Leu Cys 
        35                  40                  45              


Lys Asn Ile Lys Asp Asn Thr Asp Val Leu Ser Arg Asp Glu Phe Tyr 
    50                  55                  60                  


Leu Pro Glu Cys Asn Arg Thr Lys Leu Pro Cys His Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Pro Leu Asn Thr Ile Cys Leu Thr Cys Arg Lys Glu Leu Pro Val 
                85                  90                  95      


His Phe Ala Gly Val Gly Lys Cys Pro Glu Lys Val 
            100                 105             


<210>  26
<211>  105
<212>  PRT
<213>  Rana catesbeiana

<400>  26

Gln Asn Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Arg Asp 
1               5                   10                  15      


Val Lys Cys Asp Ala Glu Met Lys Lys Ala Leu Phe Asp Cys Lys Gln 
            20                  25                  30          


Lys Asn Thr Phe Ile Tyr Ala Arg Pro Gly Arg Val Gln Ala Leu Cys 
        35                  40                  45              


Lys Asn Ile Ile Val Ser Lys Asn Val Leu Ser Thr Asp Glu Phe Tyr 
    50                  55                  60                  


Leu Ser Asp Cys Asn Arg Ile Lys Leu Pro Cys His Tyr Lys Leu Lys 
65                  70                  75                  80  


Lys Ser Ser Asn Thr Ile Cys Ile Thr Cys Glu Asn Lys Leu Pro Val 
                85                  90                  95      


His Phe Val Ala Val Glu Glu Cys Pro 
            100                 105 


<210>  27
<211>  114
<212>  PRT
<213>  Rana pipens

<400>  27

Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His Ile 
1               5                   10                  15      


Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn Asp 
            20                  25                  30          


Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe Ile 
        35                  40                  45              


His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr Gly 
    50                  55                  60                  


Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Thr Leu Thr Thr Cys Lys 
65                  70                  75                  80  


Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe Ile 
                85                  90                  95      


Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr Gly 
            100                 105                 110         


Lys Cys 
        


<210>  28
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  28

Met Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His 
1               5                   10                  15      


Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn 
            20                  25                  30          


Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe 
        35                  40                  45              


Ile His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr 
    50                  55                  60                  


Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Thr Leu Thr Thr Cys 
65                  70                  75                  80  


Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe 
                85                  90                  95      


Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr 
            100                 105                 110         


Gly Lys Cys 
        115 


<210>  29
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  29

Met Ala Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys 
1               5                   10                  15      


His Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met 
            20                  25                  30          


Asn Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr 
        35                  40                  45              


Phe Ile His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala 
    50                  55                  60                  


Thr Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Thr Leu Thr Thr 
65                  70                  75                  80  


Cys Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn 
                85                  90                  95      


Phe Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys 
            100                 105                 110         


Thr Gly Lys Cys 
        115     


<210>  30
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  30

Ala Ala Gln Pro Ala Met Ala Lys Pro Lys Glu Asp Arg Glu Trp Glu 
1               5                   10                  15      


Lys Phe Lys Thr Lys His Ile Thr Ser Gln Ser Val Ala Asp Phe Asn 
            20                  25                  30          


Cys Asn Arg Thr Met Asn Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys 
        35                  40                  45              


Lys Pro Ile Asn Thr Phe Ile His Ser Thr Thr Gly Pro Val Lys Glu 
    50                  55                  60                  


Ile Cys Arg Arg Ala Thr Gly Arg Val Asn Lys Ser Ser Thr Gln Gln 
65                  70                  75                  80  


Phe Thr Leu Thr Thr Cys Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln 
                85                  90                  95      


Ser Asn Thr Thr Asn Phe Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro 
            100                 105                 110         


Val His Phe Val Lys Thr Gly Lys Cys 
        115                 120     


<210>  31
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  31

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys 
            20                  25                  30          


Phe Lys Thr Lys His Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys 
        35                  40                  45              


Asn Arg Thr Met Asn Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys 
    50                  55                  60                  


Pro Ile Asn Thr Phe Ile His Ser Thr Thr Gly Pro Val Lys Glu Ile 
65                  70                  75                  80  


Cys Arg Arg Ala Thr Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe 
                85                  90                  95      


Thr Leu Thr Thr Cys Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser 
            100                 105                 110         


Asn Thr Thr Asn Phe Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val 
        115                 120                 125             


His Phe Val Lys Thr Gly Lys Cys 
    130                 135     


<210>  32
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  32

Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His Ile 
1               5                   10                  15      


Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn Asp 
            20                  25                  30          


Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe Ile 
        35                  40                  45              


His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr Gly 
    50                  55                  60                  


Arg Val Asn Lys Ser Ser Cys Gln Gln Phe Thr Leu Thr Thr Cys Lys 
65                  70                  75                  80  


Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe Ile 
                85                  90                  95      


Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr Gly 
            100                 105                 110         


Lys Cys 
        


<210>  33
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  33

Met Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His 
1               5                   10                  15      


Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn 
            20                  25                  30          


Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe 
        35                  40                  45              


Ile His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr 
    50                  55                  60                  


Gly Arg Val Asn Lys Ser Ser Cys Gln Gln Phe Thr Leu Thr Thr Cys 
65                  70                  75                  80  


Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe 
                85                  90                  95      


Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr 
            100                 105                 110         


Gly Lys Cys 
        115 


<210>  34
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  34

Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His Ile 
1               5                   10                  15      


Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn Asp 
            20                  25                  30          


Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Val Asn Thr Phe Ile 
        35                  40                  45              


His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr Gly 
    50                  55                  60                  


Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Thr Leu Thr Thr Cys Lys 
65                  70                  75                  80  


Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe Ile 
                85                  90                  95      


Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr Gly 
            100                 105                 110         


Lys Cys 
        


<210>  35
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  35

Met Lys Pro Lys Glu Asp Arg Glu Trp Glu Lys Phe Lys Thr Lys His 
1               5                   10                  15      


Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Arg Thr Met Asn 
            20                  25                  30          


Asp Pro Ala Tyr Thr Pro Asp Gly Gln Cys Lys Pro Val Asn Thr Phe 
        35                  40                  45              


Ile His Ser Thr Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Thr 
    50                  55                  60                  


Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Thr Leu Thr Thr Cys 
65                  70                  75                  80  


Lys Asn Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe 
                85                  90                  95      


Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Thr 
            100                 105                 110         


Gly Lys Cys 
        115 


<210>  36
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  36

Lys Pro Lys Glu Asp Lys Glu Trp Glu Lys Phe Lys Val Lys His Ile 
1               5                   10                  15      


Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Thr Ser Thr Met Asn Asn 
            20                  25                  30          


Pro Asp Phe Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe Ile 
        35                  40                  45              


His Ser Asn Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Ser Gly 
    50                  55                  60                  


Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Pro Leu Thr Thr Cys Lys 
65                  70                  75                  80  


Asn Pro Lys Arg Cys Lys Tyr Ser Gln Ser Asn Glu Thr Asn Tyr Ile 
                85                  90                  95      


Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Ile Gly 
            100                 105                 110         


Lys Cys 
        


<210>  37
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  37

Met Lys Pro Lys Glu Asp Lys Glu Trp Glu Lys Phe Lys Val Lys His 
1               5                   10                  15      


Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Thr Ser Thr Met Asn 
            20                  25                  30          


Asn Pro Asp Phe Thr Pro Asp Gly Gln Cys Lys Pro Ile Asn Thr Phe 
        35                  40                  45              


Ile His Ser Asn Thr Gly Pro Val Lys Glu Ile Cys Arg Arg Ala Ser 
    50                  55                  60                  


Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Pro Leu Thr Thr Cys 
65                  70                  75                  80  


Lys Asn Pro Lys Arg Cys Lys Tyr Ser Gln Ser Asn Glu Thr Asn Tyr 
                85                  90                  95      


Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Ile 
            100                 105                 110         


Gly Lys Cys 
        115 


<210>  38
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  38

Lys Pro Lys Glu Asp Lys Glu Trp Val Lys Phe Lys Ala Lys His Ile 
1               5                   10                  15      


Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Lys Thr Met Asn Asp 
            20                  25                  30          


Pro Asp Phe Thr Pro Asp Gly Gln Cys Lys Pro Val Asn Thr Phe Ile 
        35                  40                  45              


His Ser Asn Thr Gly Pro Val Lys Asp Ile Cys Arg Arg Ala Ser Gly 
    50                  55                  60                  


Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Pro Leu Thr Thr Cys Asn 
65                  70                  75                  80  


Lys Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe Ile 
                85                  90                  95      


Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Ile Gly 
            100                 105                 110         


Lys Cys 
        


<210>  39
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant Amphinase-2 variant

<400>  39

Met Lys Pro Lys Glu Asp Lys Glu Trp Val Lys Phe Lys Ala Lys His 
1               5                   10                  15      


Ile Thr Ser Gln Ser Val Ala Asp Phe Asn Cys Asn Lys Thr Met Asn 
            20                  25                  30          


Asp Pro Asp Phe Thr Pro Asp Gly Gln Cys Lys Pro Val Asn Thr Phe 
        35                  40                  45              


Ile His Ser Asn Thr Gly Pro Val Lys Asp Ile Cys Arg Arg Ala Ser 
    50                  55                  60                  


Gly Arg Val Asn Lys Ser Ser Thr Gln Gln Phe Pro Leu Thr Thr Cys 
65                  70                  75                  80  


Asn Lys Pro Ile Arg Cys Lys Tyr Ser Gln Ser Asn Thr Thr Asn Phe 
                85                  90                  95      


Ile Cys Ile Thr Cys Arg Asp Asn Tyr Pro Val His Phe Val Lys Ile 
            100                 105                 110         


Gly Lys Cys 
        115 


