                         SEQUENCE LISTING

<110>  Fraunhofer-Gesellschaft zur Frderung der angewandten 
       Forschung e.V.
 
<120>  PD-L1 variants with improved affinity towards PD-1

<130>  FH16072PC

<150>  EP20172388.9
<151>  2020-04-30

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  348
<212>  DNA
<213>  Mus musculus

<400>  1
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt ggtgtactgg      120

gaaaaggaag atgagcaagt gattcagttt gtggcaggag aggaggacct taagcctcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttactgctg cataatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcccca                   348


<210>  2
<211>  348
<212>  DNA
<213>  Homo sapiens

<400>  2
gcatttactg tcacggttcc caaggaccta tatgtggtag agtatggtag caatatgaca       60

attgaatgca aattcccagt agaaaaacaa ttagacctgg ctgcactaat tgtctattgg      120

gaaatggagg ataagaacat tattcaattt gtgcatggag aggaagacct gaaggttcag      180

catagtagct acagacagag ggcccggctg ttgaaggacc agctctccct gggaaatgct      240

gcacttcaga tcacagatgt gaaattgcag gatgcagggg tgtaccgctg catgatcagc      300

tatggtggtg ccgactacaa gcgaattact gtgaaagtca atgcccca                   348


<210>  3
<211>  873
<212>  DNA
<213>  Mus musculus

<400>  3
atgaggatat ttgctggcat tatattcaca gcctgctgtc acttgctacg ggcgtttact       60

atcacggctc caaaggactt gtacgtggtg gagtatggca gcaacgtcac gatggagtgc      120

agattccctg tagaacggga gctggacctg cttgcgttag tggtgtactg ggaaaaggaa      180

gatgagcaag tgattcagtt tgtggcagga gaggaggacc ttaagcctca gcacagcaac      240

ttcaggggga gagcctcgct gccaaaggac cagcttttga agggaaatgc tgcccttcag      300

atcacagacg tcaagctgca ggacgcaggc gtttactgct gcataatcag ctacggtggt      360

gcggactaca agcgaatcac gctgaaagtc aatgccccat accgcaaaat caaccagaga      420

atttccgtgg atccagccac ttctgagcat gaactaatat gtcaggccga gggttatcca      480

gaagctgagg taatctggac aaacagtgac caccaacccg tgagtgggaa gagaagtgtc      540

accacttccc ggacagaggg gatgcttctc aatgtgacca gcagtctgag ggtcaacgcc      600

acagcgaatg atgttttcta ctgtacgttt tggagatcac agccagggca aaaccacaca      660

gcggagctga tcatcccaga actgcctgca acacatcctc cacagaacag gactcactgg      720

gtgcttctgg gatccatcct gttgttcctc attgtagtgt ccacggtcct cctcttcttg      780

agaaaacaag tgagaatgct agatgtggag aaatgtggcg ttgaagatac aagctcaaaa      840

aaccgaaatg atacacaatt cgaggagacg taa                                   873


<210>  4
<211>  116
<212>  PRT
<213>  Mus musculus

<400>  4

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val Ile 
        35                  40                  45              


Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Cys 
                85                  90                  95      


Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala Pro 
        115     


<210>  5
<211>  116
<212>  PRT
<213>  Homo sapiens

<400>  5

Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu Asp 
            20                  25                  30          


Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile 
        35                  40                  45              


Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser Tyr 
    50                  55                  60                  


Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val Lys 
            100                 105                 110         


Val Asn Ala Pro 
        115     


<210>  6
<211>  290
<212>  PRT
<213>  Mus musculus

<400>  6

Met Arg Ile Phe Ala Gly Ile Ile Phe Thr Ala Cys Cys His Leu Leu 
1               5                   10                  15      


Arg Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu 
        35                  40                  45              


Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val 
    50                  55                  60                  


Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn 
65                  70                  75                  80  


Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp 
    130                 135                 140                 


Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro 
145                 150                 155                 160 


Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly 
                165                 170                 175     


Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val 
            180                 185                 190         


Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys 
        195                 200                 205             


Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile 
    210                 215                 220                 


Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Thr His Trp 
225                 230                 235                 240 


Val Leu Leu Gly Ser Ile Leu Leu Phe Leu Ile Val Val Ser Thr Val 
                245                 250                 255     


Leu Leu Phe Leu Arg Lys Gln Val Arg Met Leu Asp Val Glu Lys Cys 
            260                 265                 270         


Gly Val Glu Asp Thr Ser Ser Lys Asn Arg Asn Asp Thr Gln Phe Glu 
        275                 280                 285             


Glu Thr 
    290 


<210>  7
<211>  23
<212>  PRT
<213>  Mus musculus

<400>  7

Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val Ile Gln Phe Val Ala 
1               5                   10                  15      


Gly Glu Glu Asp Leu Lys Pro 
            20              


<210>  8
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  8

Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile Gln Phe Val His 
1               5                   10                  15      


Gly Glu Glu Asp Leu Lys Val 
            20              


<210>  9
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  9

Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg 
1               5                   10              


<210>  10
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  10

Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg 
1               5                   10              


<210>  11
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  clone 214 encoding

<400>  11
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttatt ggtggactgg      120

gaaaaggaag atgagcaagt gattcgattt gagtcaggag aggaggacct taagcatcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttaccgctg cataatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcc                      345


<210>  12
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  clone 258 encoding

<400>  12
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt agtgtcctgg      120

gaaaaggaag atgagcaagt gattcagttt gtggccggag aggaggacct taagtttcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttacaggtg cctaatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcc                      345


<210>  13
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  clone 243 encoding

<400>  13
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt cgtggcctgg      120

gaaaaggaag atgagcacgt gattcagttt gttacaggag aggaggacct taagtttcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttaccgctg cataatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcc                      345


<210>  14
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  clone 246 encoding

<400>  14
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt ggtgggctgg      120

gaaaaggaag atgagcatgt gattcagttt gtagcaggag aggaggacct taagtttcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttacaggtg cttaatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcc                      345


<210>  15
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  clone 291 encoding

<400>  15
gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg       60

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt ggtggcctgg      120

gaaaaggaag atgagcacgt gattcagttt gtggcaggag aggaggacct taagcatcag      180

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      240

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttaccgctg cataatcagc      300

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgcc                      345


<210>  16
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  clone 214

<400>  16

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Leu Val Asp Trp Glu Lys Glu Asp Glu Gln Val Ile 
        35                  40                  45              


Arg Phe Glu Ser Gly Glu Glu Asp Leu Lys His Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala 
        115 


<210>  17
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  clone 258

<400>  17

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Val Val Ser Trp Glu Lys Glu Asp Glu Gln Val Ile 
        35                  40                  45              


Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Phe Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Leu Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala 
        115 


<210>  18
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  clone 243

<400>  18

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Val Val Ala Trp Glu Lys Glu Asp Glu His Val Ile 
        35                  40                  45              


Gln Phe Val Thr Gly Glu Glu Asp Leu Lys Phe Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala 
        115 


<210>  19
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  clone 246

<400>  19

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Val Val Gly Trp Glu Lys Glu Asp Glu His Val Ile 
        35                  40                  45              


Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Phe Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Leu Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala 
        115 


<210>  20
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  clone 291

<400>  20

Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp 
            20                  25                  30          


Leu Leu Ala Leu Val Val Ala Trp Glu Lys Glu Asp Glu His Val Ile 
        35                  40                  45              


Gln Phe Val Ala Gly Glu Glu Asp Leu Lys His Gln His Ser Asn Phe 
    50                  55                  60                  


Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys 
            100                 105                 110         


Val Asn Ala 
        115 


<210>  21
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  214-Fc

<400>  21

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val 
            20                  25                  30          


Val Glu Tyr Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu 
        35                  40                  45              


Arg Glu Leu Asp Leu Leu Ala Leu Leu Val Asp Trp Glu Lys Glu Asp 
    50                  55                  60                  


Glu Gln Val Ile Arg Phe Glu Ser Gly Glu Glu Asp Leu Lys His Gln 
65                  70                  75                  80  


His Ser Asn Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu 
                85                  90                  95      


Lys Gly Asn Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala 
            100                 105                 110         


Gly Val Tyr Arg Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg 
        115                 120                 125             


Ile Thr Leu Lys Val Asn Ala Gly Ser Arg Ser Asp Lys Thr His Thr 
    130                 135                 140                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
145                 150                 155                 160 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                165                 170                 175     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            180                 185                 190         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        195                 200                 205             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    210                 215                 220                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
225                 230                 235                 240 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                245                 250                 255     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            260                 265                 270         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        275                 280                 285             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    290                 295                 300                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
305                 310                 315                 320 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                325                 330                 335     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            340                 345                 350         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 365     


<210>  22
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  258-Fc

<400>  22

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val 
            20                  25                  30          


Val Glu Tyr Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu 
        35                  40                  45              


Arg Glu Leu Asp Leu Leu Ala Leu Val Val Ser Trp Glu Lys Glu Asp 
    50                  55                  60                  


Glu Gln Val Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Phe Gln 
65                  70                  75                  80  


His Ser Asn Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu 
                85                  90                  95      


Lys Gly Asn Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala 
            100                 105                 110         


Gly Val Tyr Arg Cys Leu Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg 
        115                 120                 125             


Ile Thr Leu Lys Val Asn Ala Gly Ser Arg Ser Asp Lys Thr His Thr 
    130                 135                 140                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
145                 150                 155                 160 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                165                 170                 175     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            180                 185                 190         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        195                 200                 205             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    210                 215                 220                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
225                 230                 235                 240 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                245                 250                 255     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            260                 265                 270         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        275                 280                 285             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    290                 295                 300                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
305                 310                 315                 320 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                325                 330                 335     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            340                 345                 350         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 365     


<210>  23
<211>  1098
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  214-Fc encoding

<400>  23
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg      120

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttatt ggtggactgg      180

gaaaaggaag atgagcaagt gattcgattt gagtcaggag aggaggacct taagcatcag      240

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      300

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttaccgctg cataatcagc      360

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgccggcag cagatctgac      420

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      480

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      540

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      600

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      660

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc      720

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg      780

cagccccgag aaccacaggt gtacaccctg cccccatccc gggaggagat gaccaagaac      840

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg      900

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac      960

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1020

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc     1080

tccctgtctc cgggtaaa                                                   1098


<210>  24
<211>  1098
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  258-Fc encoding

<400>  24
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

gcgtttacta tcacggctcc aaaggacttg tacgtggtgg agtatggcag caacgtcacg      120

atggagtgca gattccctgt agaacgggag ctggacctgc ttgcgttagt agtgtcctgg      180

gaaaaggaag atgagcaagt gattcagttt gtggccggag aggaggacct taagtttcag      240

cacagcaact tcagggggag agcctcgctg ccaaaggacc agcttttgaa gggaaatgct      300

gcccttcaga tcacagacgt caagctgcag gacgcaggcg tttacaggtg cctaatcagc      360

tacggtggtg cggactacaa gcgaatcacg ctgaaagtca atgccggcag cagatctgac      420

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      480

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      540

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      600

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      660

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc      720

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg      780

cagccccgag aaccacaggt gtacaccctg cccccatccc gggaggagat gaccaagaac      840

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg      900

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac      960

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1020

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc     1080

tccctgtctc cgggtaaa                                                   1098


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSRS linker

<400>  25

Gly Ser Arg Ser 
1               


<210>  26
<211>  290
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Arg Ile Phe Ala Val Phe Ile Phe Met Thr Tyr Trp His Leu Leu 
1               5                   10                  15      


Asn Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
            20                  25                  30          


Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
        35                  40                  45              


Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
    50                  55                  60                  


Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
65                  70                  75                  80  


Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
                85                  90                  95      


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
            100                 105                 110         


Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
        115                 120                 125             


Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
    130                 135                 140                 


Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
145                 150                 155                 160 


Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
                165                 170                 175     


Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
            180                 185                 190         


Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
        195                 200                 205             


Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
    210                 215                 220                 


Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr His 
225                 230                 235                 240 


Leu Val Ile Leu Gly Ala Ile Leu Leu Cys Leu Gly Val Ala Leu Thr 
                245                 250                 255     


Phe Ile Phe Arg Leu Arg Lys Gly Arg Met Met Asp Val Lys Lys Cys 
            260                 265                 270         


Gly Ile Gln Asp Thr Asn Ser Lys Lys Gln Ser Asp Thr His Leu Glu 
        275                 280                 285             


Glu Thr 
    290 


