                               SEQUENCE LISTING

<110> REGENTS OF THE UNIVERSITY OF MINNESOTA
      PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> CAS9-BASED DIAGNOSTIC ASSAY AND METHODS OF USING

<130> 0110.000653WO01

<140>
<141>

<150> 63/018,933
<151> 2020-05-01

<160> 71    

<170> PatentIn version 3.5

<210> 1
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
taacaaacat gctgattttg acacatgg                                          28


<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 2
ccaggcacga caaaacccac                                                   20


<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
ctaaatcacc cattcagtac atcgatatcg                                        30


<210> 4
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
cttcatagaa cgaacaacgc actacaagac tacc                                   34


<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
gaattgtgcg tggatgaggc tgg                                               23


<210> 6
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
caacacgaac gtcatgatac tc                                                22


<210> 7
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
auuaauugua aaaggugaac                                                   20


<210> 8
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
auuaauugua aaagguaaac                                                   20


<210> 9
<211> 20
<212> DNA
<213> Severe acute respiratory syndrome coronavirus 2

<400> 9
attaattgta aaaggtaaac                                                   20


<210> 10
<211> 20
<212> DNA
<213> Severe acute respiratory syndrome coronavirus 2

<400> 10
attaattgta aaaggtgaac                                                   20


<210> 11
<211> 23
<212> DNA
<213> Severe acute respiratory syndrome coronavirus 2

<400> 11
attaattgta aaaggtaaac agg                                               23


<210> 12
<211> 23
<212> DNA
<213> Severe acute respiratory syndrome coronavirus 2

<400> 12
attaattgta aaaggtgaac agg                                               23


<210> 13
<211> 184
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 13
gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc tgattttgac       60

acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc attgattgct      120

gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac gatattacgc      180

acaa                                                                   184


<210> 14
<211> 120
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 14
uaacaaacau gcugauuuug acacaugguu uagccagcgu ggugguaguu auacuaauga       60

caaagcuugc ccauugauug cugcagucau aacaagagaa guggguuuug ucgugccugg      120


<210> 15
<211> 175
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 15
gaattgtgcg tggatgaggc tggttctaaa tcacccattc agtacatcga tatcggtaat       60

tatacagttt cctgtttacc ttttacaatt aattgccagg aacctaaatt gggtagtctt      120

gtagtgcgtt gttcgttcta tgaagacttt ttagagtatc atgacgttcg tgttg           175


<210> 16
<211> 120
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 16
cuaaaucacc cauucaguac aucgauaucg guaauuauac aguuuccugu uuaccuuuua       60

caauuaauug ccaggaaccu aaauugggua gucuuguagu gcguuguucg uucuaugaag      120


<210> 17
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
taacaaacat gctgattttg acacatgg                                          28


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
ccaggcacga caaaacccac                                                   20


<210> 19
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ctaaatcacc cattcagtac atcgatatcg                                        30


<210> 20
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
cttcatagaa cgaacaacgc actacaagac tacc                                   34


<210> 21
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
gaattgtgcg tggatgaggc tgg                                               23


<210> 22
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
ucagaacagc aacauuugaa                                                   20


<210> 23
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
cgggagggtg ggcgggaggg tgggcgggag ggtgggcggg agggtggg                    48


<210> 24
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
cccaccctcc cgcccaccct cccgcccacc ctcccgccca ccctcccg                    48


<210> 25
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 25
ggagggtggg gattaattgt aaaaggtgaa cgggcgggag ggtgg                       45


<210> 26
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 26
ccaccctccc gcccgttcac cttttacaat taatccccac cctcc                       45


<210> 27
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
ggagggtggg gattaattgt aaaaggtaaa cgggcgggag ggtgg                       45


<210> 28
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
ccaccctccc gcccgtttac cttttacaat taatccccac cctcc                       45


<210> 29
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
gtcactcgtg acatagcatc tacag                                             25


<210> 30
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
ttgtgcgtaa tatcgtgcca gg                                                22


<210> 31
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
ttgtgcgtaa tatcgtgcca gg                                                22


<210> 32
<211> 120
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 32
taacaaacat gctgattttg acacatggtt tagccagcgt ggtggtagtt atactaatga       60

caaagcttgc ccattgattg ctgcagtcat aacaagagaa gtgggttttg tcgtgcctgg      120


<210> 33
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 33
taatacgact cactataggg acacatggtt tagtcagcgg ttttagagct agaaatagca       60

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      120

tt                                                                     122


<210> 34
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 34
taatacgact cactataggg acacatggtt tagccagcgg ttttagagct agaaatagca       60

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      120

tt                                                                     122


<210> 35
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 35
taatacgact cactatagg                                                    19


<210> 36
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
aaaaaaagca ccgactcggt gccac                                             25


<210> 37
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
caacacgaac gtcatgatac tc                                                22


<210> 38
<211> 120
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 38
ctaaatcacc cattcagtac atcgatatcg gtaattatac agtttcctgt ttacctttta       60

caattaattg ccaggaacct aaattgggta gtcttgtagt gcgttgttcg ttctatgaag      120


<210> 39
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
cttcatagaa cgaacaacgc actacaagac tacc                                   34


<210> 40
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 40
taatacgact cactatagga ttaattgtaa aaggtgaacg ttttagagct agaaatagca       60

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      120

tt                                                                     122


<210> 41
<211> 122
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 41
taatacgact cactatagga ttaattgtaa aaggtaaacg ttttagagct agaaatagca       60

agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt      120

tt                                                                     122


<210> 42
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
taatacgact cactataggt aacaaacatg ctgattttga cacatgg                     47


<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 43
atatcgccgc gctcgtcgtc                                                   20


<210> 44
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 44
atgtcgtccc agttggtgac g                                                 21


<210> 45
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 45
atgtcgtccc agttggtgac g                                                 21


<210> 46
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 46
atatcgccgc gctcgtcgtc                                                   20


<210> 47
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 47
atatcgccgc gctcgtcgtc                                                   20


<210> 48
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 48
atgtcgtccc agttggtgac g                                                 21


<210> 49
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 49
taatacgact cactataggg tcactcgtga catagcatct acag                        44


<210> 50
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 50
taatacgact cactataggg aattgtgcgt ggatgaggct gg                          42


<210>  51
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
      oligonucleotide

<400>  51
cucaccgugc ccagugagcg                                                  20

<210>  52
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
      oligonucleotide

<400>  52
aauucgagca gcugaaacug                                                  20

<210>  53
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
      oligonucleotide

<400>  53
uugaacagca gcuguguaug                                                  20

<210>  54
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
      oligonucleotide

<400>  54
cacuggcugu cgcuucucaa                                                 20

<210>  55
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic
      oligonucleotide

<400>  55
ttagatgaaa tctaaaacaa cacg                                          24

<210>  56
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic SARS-CoV-2 
PCR template containing a cytosine SNP at position 28144

<400>  56
gaattgtgcg tggatgaggc tggttctaaa tcacccattc agtacatcga tatcggtaat    60
tatacagtt tcctgtttcac cttttacaat taattgccag gaacctaaat tgggtagtct    120
tgtagtgcgt tgttcgttct atgaagactt tttagagtat catgacgttc gtgttg        176

<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Forward primer for 
Influenza A amplification from synthetic DNA

<400>  57
gcattytgga caaavcgtct acg                                          23

<210>  58
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Reverse primer for 
Influenza A amplification from synthetic DNA

<400>  58
gcattytgga caaavcgtct acg                                         23

<210>  59
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic PCR template
to generate Influenza A DNA amplicons

<400>  59
gctctcatgg aatggctaaa gacaagacca atcttgtcac ctctgactaa ggggatttta      60
ggatttgtgt tcacgctcac cgtgcccagt gagcgaggac tgcagcgtag acgctttgtc      120
caaaatgccc taaatgggaa tggggacccg aacaacatgg                            160	
		
<210>  60
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Forward primer for 
Influenza B amplification from synthetic DNA

<400>  60
tcctcaaytc actcttcgag gcg                                              23	

<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Reverse primer for 
Influenza B amplification from synthetic DNA

<400>  61
cggtgctctt gaccaaattg g                                               21

<210>  62
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic PCR template 
to generate Influenza B DNA amplicons

<400>  62
tacagtggag gatgaagaag atggccatcg gatcctcaat tcactcttcg agcgtcttaa       60
tgaaggacat tcaaagccaa ttcgagcagc tgaaactgcg gtgggagtct tatcccaatt       120
tggtcaagag caccgactat caccagaaga gggagacaat                             160

<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Forward primer for 
RSV amplification from synthetic DNA

<400>  63
ggcaaatatg gaaacatacg tgaa                                               24

<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Reverse primer for 
RSV amplification from synthetic DNA

<400>  64
catgggcacc catattgtaa                                                   20

<210>  65
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic PCR 
template to generate RSV DNA amplicons

<400>  65
ggcaaatatg gaaacatacg tgaacaagct tcacgaaggc tccacataca cagctgctgt       60
tcaatacaat gtcctagaaa aagacgatga ccctgcatca cttacaatat gggtgcccat       120
gttccaatca                                                              130

<210>  66
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: FAM fluorescent probe 
with Iowa Black quencher that recognizes SARS-CoV-2 with a 
thymine at position 28144

<400>  66
tcctgtttac cttttacaat taattgccag ga                                     32

<210>  67
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: FAM fluorescent probe 
with Iowa Black quencher that recognizes SARS-CoV-2 
with a cytosine at position 28144

<400>  67
tcctgttcac cttttacaat taattgccag ga                                     32 

<210>  68
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Texas Red fluorescent 
probe with Iowa Black quencher that recognizes 
Influenza A sequence

<400>  68
cagtcctcgc tcactgggca cggtgagcgt ga                                    32

<210>  69
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Yakima Yellow 
fluorescent probe with Iowa Black quencher 
that recognizes Influenza B sequence

<400>  69
tcccaccgca gtttcagctg ctcgaattgg ct                                  32

<210>  70
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Cy3 fluorescent 
probe with Iowa Black quencher that 
recognizes RSV sequence

<400>  70
gctccacata cacagctgct gttcaataca at                                     32

<210>  71
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: TAMRA fluorescent 
probe with Iowa Black quencher 
that recognizes RSV sequence

<400>  71
gctccacata cacagctgct gttcaataca at                                      32
