                         SEQUENCE LISTING

<110>  Wayne State University
 
<120>  ANTIVIRAL COMPOSITIONS AND METHODS

<130>  47WAY14652WO

<150>  63/021,747
<151>  2020-05-08

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  181
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Gly Ser Arg Glu Val Val Ala Met Asp Cys Glu Met Val Gly 
1               5                   10                  15      


Leu Gly Pro His Arg Glu Ser Gly Leu Ala Arg Cys Ser Leu Val Asn 
            20                  25                  30          


Val His Gly Ala Val Leu Tyr Asp Lys Phe Ile Arg Pro Glu Gly Glu 
        35                  40                  45              


Ile Thr Asp Tyr Arg Thr Arg Val Ser Gly Val Thr Pro Gln His Met 
    50                  55                  60                  


Val Gly Ala Thr Pro Phe Ala Val Ala Arg Leu Glu Ile Leu Gln Leu 
65                  70                  75                  80  


Leu Lys Gly Lys Leu Val Val Gly His Asp Leu Lys His Asp Phe Gln 
                85                  90                  95      


Ala Leu Lys Glu Asp Met Ser Gly Tyr Thr Ile Tyr Asp Thr Ser Thr 
            100                 105                 110         


Asp Arg Leu Leu Trp Arg Glu Ala Lys Leu Asp His Cys Arg Arg Val 
        115                 120                 125             


Ser Leu Arg Val Leu Ser Glu Arg Leu Leu His Lys Ser Ile Gln Asn 
    130                 135                 140                 


Ser Leu Leu Gly His Ser Ser Val Glu Asp Ala Arg Ala Thr Met Glu 
145                 150                 155                 160 


Leu Tyr Gln Ile Ser Gln Arg Ile Arg Ala Arg Arg Gly Leu Pro Arg 
                165                 170                 175     


Leu Ala Val Ser Asp 
            180     


<210>  2
<211>  546
<212>  DNA
<213>  Homo sapiens

<400>  2
atggctggga gccgtgaggt ggtggccatg gactgcgaga tggtggggct ggggccccac       60

cgggagagtg gcctggctcg ttgcagcctc gtgaacgtcc acggtgctgt gctgtacgac      120

aagttcatcc ggcctgaggg agagatcacc gattacagaa cccgggtcag cggggtcacc      180

cctcagcaca tggtgggggc cacaccattt gccgtggcca ggctagagat cctgcagctc      240

ctgaaaggca agctggtggt gggtcatgac ctgaagcacg acttccaggc actgaaagag      300

gacatgagcg gctacacaat ctacgacacg tccactgaca ggctgttgtg gcgtgaggcc      360

aagctggacc actgcaggcg tgtctccctg cgggtgctga gtgagcgcct cctgcacaag      420

agcatccaga acagcctgct tggacacagc tcggtggaag atgcgagggc aacgatggag      480

ctctatcaaa tctcccagag aatccgagcc cgccgagggc tgccccgcct ggctgtgtca      540

gattaa                                                                 546


<210>  3
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide for use in CRISPR-mediated knockout

<400>  3
caccgcagca ccgtggacgt tcacg                                             25


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide for use in CRISPR-mediated knockout

<400>  4
aaaccgtgaa cgtccacggt gctgc                                             25


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  5

Gly Ser Gly Ser Gly 
1               5   


<210>  6
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer for human interferon-stimulated gene 20-kDa 
       protein (human ISG20)

<400>  6
ccccaaagcc caaagacact ctgtatatca ccagggagcc tgaagttaca tgcgtcgttg       60

t                                                                       61


<210>  7
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutagenesis primer for human interferon-stimulated gene 20-kDa 
       protein (human ISG20)

<400>  7
acaacgacgc atgtaacttc aggctccctg gtgatataca gagtgtcttt gggctttggg       60

g                                                                       61


<210>  8
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer for Zika virus

<400>  8
ccgctgccca acacaag                                                      17


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer for Zika virus

<400>  9
ccactaacgt tcttttgcag acat                                              24


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for Zika virus nucleic acid

<400>  10
agcctacctt gacaagcagt cagacactca a                                      31


<210>  11
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-tag

<400>  11

Met His His His His His His Ser Ser Leu Ala 
1               5                   10      


<210>  12
<211>  452
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein including including ISG20 and Fc portion of human 
       IgG

<400>  12

Met Arg Gly Pro Ala Val Leu Leu Thr Val Ala Leu Ala Thr Leu Leu 
1               5                   10                  15      


Ala Pro Gly Ala Gly Ala His His His His His His His His His His 
            20                  25                  30          


Glu Asn Leu Tyr Phe Gln Gly Met Ala Gly Ser Arg Glu Val Val Ala 
        35                  40                  45              


Met Asp Cys Glu Met Val Gly Leu Gly Pro His Arg Glu Ser Gly Leu 
    50                  55                  60                  


Ala Arg Cys Ser Leu Val Asn Val His Gly Ala Val Leu Tyr Asp Lys 
65                  70                  75                  80  


Phe Ile Arg Pro Glu Gly Glu Ile Thr Asp Tyr Arg Thr Arg Val Ser 
                85                  90                  95      


Gly Val Thr Pro Gln His Met Val Gly Ala Thr Pro Phe Ala Val Ala 
            100                 105                 110         


Arg Leu Glu Ile Leu Gln Leu Leu Lys Gly Lys Leu Val Val Gly His 
        115                 120                 125             


Asp Leu Lys His Asp Phe Gln Ala Leu Lys Glu Asp Met Ser Gly Tyr 
    130                 135                 140                 


Thr Ile Tyr Asp Thr Ser Thr Asp Arg Leu Leu Trp Arg Glu Ala Lys 
145                 150                 155                 160 


Leu Asp His Cys Arg Arg Val Ser Leu Arg Val Leu Ser Glu Arg Leu 
                165                 170                 175     


Leu His Lys Ser Ile Gln Asn Ser Leu Leu Gly His Ser Ser Val Glu 
            180                 185                 190         


Asp Ala Arg Ala Thr Met Glu Leu Tyr Gln Ile Ser Gln Arg Ile Arg 
        195                 200                 205             


Ala Arg Arg Gly Leu Pro Arg Leu Ala Val Ser Asp Gly Ser Gly Ser 
    210                 215                 220                 


Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
225                 230                 235                 240 


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                245                 250                 255     


Tyr Ile Thr Arg Glu Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            260                 265                 270         


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        275                 280                 285             


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    290                 295                 300                 


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
305                 310                 315                 320 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                325                 330                 335     


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            340                 345                 350         


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
        355                 360                 365             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    370                 375                 380                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
385                 390                 395                 400 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                405                 410                 415     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            420                 425                 430         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        435                 440                 445             


Ser Pro Gly Lys 
    450         


<210>  13
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal Sequence

<400>  13

Met Arg Gly Pro Ala Val Leu Leu Thr Val Ala Leu Ala Thr Leu Leu 
1               5                   10                  15      


Ala Pro Gly Ala Gly Ala 
            20          


<210>  14
<211>  7
<212>  PRT
<213>  Tobacco etch virus

<400>  14

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  15
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  15

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  16
<211>  227
<212>  PRT
<213>  Homo sapiens

<400>  16

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  17
<211>  227
<212>  PRT
<213>  Homo sapiens

<400>  17

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Tyr 
            20                  25                  30          


Ile Thr Arg Glu Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


