               SEQUENCE LISTING

<110> SYNHELIX
      RANDRIANJATOVO-GBALOU Irina
      SAID Ahmed
      RAHIER Renaud

<120> CONTROLLED TEMPLATE-INDEPENDENT SYNTHESIS OF NUCLEIC 
      ACIDS USING THERMOSTABLE ENZYMES

<130> IBIO-1583/PCT

<150> US63/038,168
<151> 2020-06-12
<150> EP20305903.5
<151> 2020-08-06

<160> 16

<170> BiSSAP 1.3.6

<210> 1
<211> 700
<212> PRT
<213> Pyrococcus sp. 12/1


<220> 
<223> Pyrococcus sp. 12-1 DNA primase

<400> 1
Met Arg Pro Ser Asp Ile Ile Ile Asp Val Tyr Lys Ala Ile Gln Asp 
1               5                   10                  15      
His Pro Gly Ala Gly Lys Leu Ala Ile Glu Leu Arg Phe Tyr Pro Arg 
            20                  25                  30          
Pro Thr Ser Glu Trp Ile Ile Val Ala Asp Ile Glu Asp Lys Ala Glu 
        35                  40                  45              
Glu Leu His Lys Val Leu Phe Lys Asn Asn Val Leu Gly Lys Lys Glu 
    50                  55                  60                  
Ala Tyr Ile Ser Met Ala Leu His Asp Phe Glu Glu Val Gly Lys Lys 
65                  70                  75                  80  
Leu Glu Lys Leu Arg Glu Leu Glu Glu Glu Arg Ala Gln Lys Glu Gly 
                85                  90                  95      
Arg Lys Pro Arg Glu Val Thr Leu Arg Asn Val Gln Gly Glu Ala Thr 
            100                 105                 110         
Gly Lys Val His Lys Thr Val Ser Lys Tyr Thr Leu Thr Leu Val Val 
        115                 120                 125             
Asp Ile Asp Val Glu Glu Ile His Lys Ser Lys Val Val Glu Ser Glu 
    130                 135                 140                 
Glu Lys Ala Phe Glu Leu Ala Lys Arg Ala Trp Asp Glu Leu Lys Pro 
145                 150                 155                 160 
Lys Leu Glu Gly Ile Gly Val Lys Pro Arg Tyr Val Phe Phe Thr Gly 
                165                 170                 175     
Gly Gly Val Gln Leu Trp Phe Val Ala Pro Gly Leu Glu Pro Ile Glu 
            180                 185                 190         
Val Ile Asp Arg Ala Ser Arg Val Ile Pro Pro Val Leu Asn Ala Met 
        195                 200                 205             
Leu Pro Glu Gly Tyr Ser Val Asp Asn Ile Phe Asp Arg Ala Arg Ile 
    210                 215                 220                 
Val Arg Val Pro Leu Thr Ile Asn Tyr Lys Tyr Lys Thr Pro Asp Glu 
225                 230                 235                 240 
Arg Pro Leu Glu Ile Arg Gly Arg Leu Ile Glu Phe Asn Asp Val Arg 
                245                 250                 255     
Thr Pro Leu Gly Glu Val Leu Asp Lys Leu Glu Ala Tyr Ala Lys Glu 
            260                 265                 270         
His Gly Ile Ser Leu Val Thr Pro Ser Gln Ala Arg Phe Ile Gly Thr 
        275                 280                 285             
Val Gly Arg Tyr Glu Val Asp Lys Gly Asp Phe Arg Val Leu Ala Glu 
    290                 295                 300                 
Arg Leu Val Gln Glu Leu Ala Pro Trp Phe Arg Arg Val Lys Glu Lys 
305                 310                 315                 320 
Gly Gly Ser Arg His His Leu Val Asn Ala Ile Ala Ala Tyr Ile Ala 
                325                 330                 335     
Arg Asn Thr Asn Leu Thr Phe Glu Asp Leu Ala Lys Ile Trp Glu Ile 
            340                 345                 350         
Val His Ala Glu Leu Val Arg Leu Gly Leu Glu Asp Pro Asn Asp Trp 
        355                 360                 365             
Ser Asn Arg Tyr His Thr Ile Lys Asp Val Tyr Glu Lys Leu Gln Ala 
    370                 375                 380                 
Gly Thr Phe Leu Gly Thr Arg Ala Tyr Met Leu Lys Tyr Leu Asn Met 
385                 390                 395                 400 
Met Ser Glu Glu Glu Ile Val Glu Ile Leu Arg Ala Val Lys Arg Ala 
                405                 410                 415     
Leu Phe Pro Tyr Leu Lys Pro Val Asn Ala Gly Phe Val Ser Lys Phe 
            420                 425                 430         
Val Asp Glu Pro Tyr Gly Arg Asp Glu Ala Pro Lys Lys Trp Glu Asp 
        435                 440                 445             
Val Asp Glu Asp Arg Arg Lys Ala Val Gly Val Phe Phe Ile Arg Ala 
    450                 455                 460                 
Leu Ala Phe Glu Thr Ala Glu Tyr Ile Tyr Val Glu Asp Leu Pro Lys 
465                 470                 475                 480 
Pro Lys Thr Phe Ser Ile Lys Ser His Ser Glu Asp Gly Lys Glu Lys 
                485                 490                 495     
Leu Arg Phe Asn Glu Ala Leu Trp Gln Ser Phe Leu Ser Trp Leu Gly 
            500                 505                 510         
Val Gln Glu Gly Glu Pro Phe Asp Arg Val Asp Phe Glu Asn Leu Leu 
        515                 520                 525             
Ile Glu Lys Phe Gly Ile Thr Glu His Glu Leu Arg Ser Ile Tyr Phe 
    530                 535                 540                 
Ser Lys Val Leu Tyr Leu Leu Thr Pro Glu Gly Met Arg Met Pro Lys 
545                 550                 555                 560 
Cys Ile Val Glu Phe Leu Lys Glu Leu Ala Asp Glu Gly Asn Leu Ser 
                565                 570                 575     
Asp Glu Lys Val Ala His Leu Ala His Trp Ile Lys Tyr Phe Ala Lys 
            580                 585                 590         
Pro Leu Arg His Ser Thr Thr Ser Ile Met Leu Arg Ala Gln Gly Lys 
        595                 600                 605             
Pro Val Asp Met Arg Met Ala Val Trp Ala Lys Ile Val Glu Phe Phe 
    610                 615                 620                 
Ala Glu Asp Asp Asp Val Ala Lys Arg Leu Val Ser Val Phe Lys Lys 
625                 630                 635                 640 
Ala Tyr Glu Glu Ala Glu Pro Pro Phe Pro Cys Phe Gly Val Lys Glu 
                645                 650                 655     
Cys Pro Phe Phe Pro Gly His Lys Gly Cys Pro Phe Ile Ala Pro Lys 
            660                 665                 670         
Arg Asn Glu Ile Leu Ala Val Ser Leu Val Asp Val Gln Ile His Gly 
        675                 680                 685             
Thr Asp Gly Ile Val Val Val Val Gly Gly Gly Glu 
    690                 695                 700 

<210> 2
<211> 296
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpP12Δ297-898

<400> 2
Met Arg Pro Ser Asp Ile Ile Ile Asp Val Tyr Lys Ala Ile Gln Asp 
1               5                   10                  15      
His Pro Gly Ala Gly Lys Leu Ala Ile Glu Leu Arg Phe Tyr Pro Arg 
            20                  25                  30          
Pro Thr Ser Glu Trp Ile Ile Val Ala Asp Ile Glu Asp Lys Ala Glu 
        35                  40                  45              
Glu Leu His Lys Val Leu Phe Lys Asn Asn Val Leu Gly Lys Lys Glu 
    50                  55                  60                  
Ala Tyr Ile Ser Met Ala Leu His Asp Phe Glu Glu Val Gly Lys Lys 
65                  70                  75                  80  
Leu Glu Lys Leu Arg Glu Leu Glu Glu Glu Arg Ala Gln Lys Glu Gly 
                85                  90                  95      
Arg Lys Pro Arg Glu Val Thr Leu Arg Asn Val Gln Gly Glu Ala Thr 
            100                 105                 110         
Gly Lys Val His Lys Thr Val Ser Lys Tyr Thr Leu Thr Leu Val Val 
        115                 120                 125             
Asp Ile Asp Val Glu Glu Ile His Lys Ser Lys Val Val Glu Ser Glu 
    130                 135                 140                 
Glu Lys Ala Phe Glu Leu Ala Lys Arg Ala Trp Asp Glu Leu Lys Pro 
145                 150                 155                 160 
Lys Leu Glu Gly Ile Gly Val Lys Pro Arg Tyr Val Phe Phe Thr Gly 
                165                 170                 175     
Gly Gly Val Gln Leu Trp Phe Val Ala Pro Gly Leu Glu Pro Ile Glu 
            180                 185                 190         
Val Ile Asp Arg Ala Ser Arg Val Ile Pro Pro Val Leu Asn Ala Met 
        195                 200                 205             
Leu Pro Glu Gly Tyr Ser Val Asp Asn Ile Phe Asp Arg Ala Arg Ile 
    210                 215                 220                 
Val Arg Val Pro Leu Thr Ile Asn Tyr Lys Tyr Lys Thr Pro Asp Glu 
225                 230                 235                 240 
Arg Pro Leu Glu Ile Arg Gly Arg Leu Ile Glu Phe Asn Asp Val Arg 
                245                 250                 255     
Thr Pro Leu Gly Glu Val Leu Asp Lys Leu Glu Ala Tyr Ala Lys Glu 
            260                 265                 270         
His Gly Ile Ser Leu Val Thr Pro Ser Gln Ala Arg Phe Ile Gly Thr 
        275                 280                 285             
Val Gly Arg Tyr Glu Val Asp Lys 
    290                 295     

<210> 3
<211> 290
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpP12Δ87-92Δ297-898

<400> 3
Met Arg Pro Ser Asp Ile Ile Ile Asp Val Tyr Lys Ala Ile Gln Asp 
1               5                   10                  15      
His Pro Gly Ala Gly Lys Leu Ala Ile Glu Leu Arg Phe Tyr Pro Arg 
            20                  25                  30          
Pro Thr Ser Glu Trp Ile Ile Val Ala Asp Ile Glu Asp Lys Ala Glu 
        35                  40                  45              
Glu Leu His Lys Val Leu Phe Lys Asn Asn Val Leu Gly Lys Lys Glu 
    50                  55                  60                  
Ala Tyr Ile Ser Met Ala Leu His Asp Phe Glu Glu Val Gly Lys Lys 
65                  70                  75                  80  
Leu Glu Lys Leu Arg Glu Gln Lys Glu Gly Arg Lys Pro Arg Glu Val 
                85                  90                  95      
Thr Leu Arg Asn Val Gln Gly Glu Ala Thr Gly Lys Val His Lys Thr 
            100                 105                 110         
Val Ser Lys Tyr Thr Leu Thr Leu Val Val Asp Ile Asp Val Glu Glu 
        115                 120                 125             
Ile His Lys Ser Lys Val Val Glu Ser Glu Glu Lys Ala Phe Glu Leu 
    130                 135                 140                 
Ala Lys Arg Ala Trp Asp Glu Leu Lys Pro Lys Leu Glu Gly Ile Gly 
145                 150                 155                 160 
Val Lys Pro Arg Tyr Val Phe Phe Thr Gly Gly Gly Val Gln Leu Trp 
                165                 170                 175     
Phe Val Ala Pro Gly Leu Glu Pro Ile Glu Val Ile Asp Arg Ala Ser 
            180                 185                 190         
Arg Val Ile Pro Pro Val Leu Asn Ala Met Leu Pro Glu Gly Tyr Ser 
        195                 200                 205             
Val Asp Asn Ile Phe Asp Arg Ala Arg Ile Val Arg Val Pro Leu Thr 
    210                 215                 220                 
Ile Asn Tyr Lys Tyr Lys Thr Pro Asp Glu Arg Pro Leu Glu Ile Arg 
225                 230                 235                 240 
Gly Arg Leu Ile Glu Phe Asn Asp Val Arg Thr Pro Leu Gly Glu Val 
                245                 250                 255     
Leu Asp Lys Leu Glu Ala Tyr Ala Lys Glu His Gly Ile Ser Leu Val 
            260                 265                 270         
Thr Pro Ser Gln Ala Arg Phe Ile Gly Thr Val Gly Arg Tyr Glu Val 
        275                 280                 285             
Asp Lys 
    290 

<210> 4
<211> 928
<212> PRT
<213> Thermococcus sp. CIR10


<220> 
<223> Thermococcus sp. CIR10 DNA primase

<400> 4
Met Ser Gly Arg Glu Phe Lys Arg Pro Ser Asp Val Ile Ile Asp Ile 
1               5                   10                  15      
Tyr Lys Val Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu 
            20                  25                  30          
Phe Arg Phe Tyr Pro Tyr Pro Thr Ser Glu Trp Ile Leu Leu Asn Asp 
        35                  40                  45              
Ile Glu Asp Lys Ala Arg Glu Ile Asp Lys Val Leu Phe Lys Asn Asn 
    50                  55                  60                  
Ile Leu Gly Lys Lys Glu Ala Tyr Ile Ser Met Ala Ile His Asp Phe 
65                  70                  75                  80  
Asp Glu Val Thr Lys Lys Leu Glu Lys Leu Gln Glu Leu Glu His Glu 
                85                  90                  95      
Lys Ala Gln Lys Glu Gly Arg Gln Pro Lys Glu Ile Thr Leu Arg His 
            100                 105                 110         
Val Gln Gly Glu Ala Thr Gly Lys Ile His Thr Thr Val Ser Ser Tyr 
        115                 120                 125             
Thr Leu Thr Leu Val Val Asp Ile Asp Val Asn Glu Ile His Asp Ser 
    130                 135                 140                 
Lys Ala Val Glu Ser Glu Glu Lys Ala Leu Glu Val Ser Lys Arg Ala 
145                 150                 155                 160 
Trp Glu Val Leu Lys Pro Asn Leu Glu Glu Leu Gly Ile Lys Pro Arg 
                165                 170                 175     
Tyr Val Phe Phe Thr Gly Gly Gly Ile Gln Leu Trp Phe Val Ala Pro 
            180                 185                 190         
Glu Pro Glu Asn Ile Ser Val Ile Asp Lys Ala Ala Glu Ile Ile Pro 
        195                 200                 205             
Pro Val Leu Asn Thr Leu Leu Pro Glu Gly Tyr Ser Val Asp Asn Ile 
    210                 215                 220                 
Phe Asp Arg Ala Arg Ile Val Arg Val Pro Phe Thr Val Asn Tyr Lys 
225                 230                 235                 240 
Tyr Lys Thr Pro Asp Gly Lys Pro Leu Glu Leu Arg Gly Arg Leu Leu 
                245                 250                 255     
Glu Phe Asn Asp Val Arg Thr Pro Leu Gly Asp Ile Leu Glu Lys Leu 
            260                 265                 270         
Glu Ala Tyr Ala Lys Gly His Lys Ile Ser Leu Gly Ser Thr Ser Arg 
        275                 280                 285             
Ser Gly Lys Phe Arg Gly Val Ala Gly Arg Tyr Glu Val Lys Lys Glu 
    290                 295                 300                 
Asn Phe Glu Glu Leu Ala Lys Arg Leu Val Glu Glu Leu Ala Pro Trp 
305                 310                 315                 320 
Phe Lys Lys Ile Lys Glu Arg Gly Gly Ser Arg His His Leu Val Asn 
                325                 330                 335     
Ala Ile Ala Ala Tyr Ile Ala Arg Asn Thr Asn Leu Thr Glu Glu Asp 
            340                 345                 350         
Leu Leu Gly Lys Asp Gln Glu Asp Gly Thr His Val Val Gly Leu Trp 
        355                 360                 365             
Glu Leu Val His Ser Lys Leu Val Glu Leu Gly Leu Glu Asp Pro Gly 
    370                 375                 380                 
Asp Trp Ser Asn Arg Tyr His Thr Ile Lys Asp Val Tyr Glu Lys Leu 
385                 390                 395                 400 
Tyr Ala Gly Thr Thr Leu Gly Thr Arg Ala Tyr Met Met Lys Tyr Leu 
                405                 410                 415     
Asn Val Ser Glu Glu Glu Ala Ile Glu Ile Leu Arg Ser Val Lys Arg 
            420                 425                 430         
Ala Leu Phe Pro Tyr Leu His Pro Val Asn Val Gln Val Ile Ser Lys 
        435                 440                 445             
Phe Glu Ala Lys Pro Tyr Ser Lys Glu Glu Ala Pro Thr Glu Trp Glu 
    450                 455                 460                 
Ala Val Asp Glu Asp Arg Lys Lys Ala Val Gly Ile Trp Tyr Ile Glu 
465                 470                 475                 480 
Val Leu Ala Leu Glu Thr Ala Asn Tyr Val Tyr Ile Glu Asp Leu Ser 
                485                 490                 495     
Lys Pro Gly Val Phe Tyr Ile Val Glu Lys Val Lys Arg Thr Val Lys 
            500                 505                 510         
Val Gly Lys Lys Glu Lys Gly Val Glu Val Asp Glu Tyr His Phe Asn 
        515                 520                 525             
Pro Ala Leu Trp Gln Ser Phe Leu Asn Trp Leu Gly Ile Lys Glu Gly 
    530                 535                 540                 
Glu Pro Ile Glu Arg Glu Glu Leu Trp Asn Leu Leu Leu Glu Lys Phe 
545                 550                 555                 560 
Asp Ile Lys Asp Tyr Glu Leu Arg Ala Ile Tyr Phe Arg Lys Ile Leu 
                565                 570                 575     
His Leu Leu Ser Pro Glu Gly Met Arg Arg Pro Arg Cys Val Glu Glu 
            580                 585                 590         
Phe Leu Arg Glu Leu Ala Asp Glu Gly Phe Leu Ser Glu Asp Lys Val 
        595                 600                 605             
Arg His Leu Ala His Trp Ile Lys Phe Tyr Ala Lys Pro Leu Arg His 
    610                 615                 620                 
Ser Thr Thr Ser Ile Met Leu Arg Ala Lys Gly Lys Pro Val Asp Met 
625                 630                 635                 640 
Arg Met Ala Val Trp Ala Lys Val Val Glu Phe Phe Ala Glu Asp Glu 
                645                 650                 655     
Glu Thr Ala Gln Gly Leu Ile Glu Thr Phe Arg Glu Ala Tyr Glu Gln 
            660                 665                 670         
Ala Glu Pro Pro Phe Pro Cys Phe Gly Ala Arg Glu Cys Pro Phe Phe 
        675                 680                 685             
Gln Glu His Arg Gly Cys Pro Phe Ile Ala Pro Lys Arg Asp Glu Ile 
    690                 695                 700                 
Leu Ala Val Ser Leu Val Asp Val Gln Leu His Gly Ser Asp Gly Ile 
705                 710                 715                 720 
Val Ile Ile Val Gly Ser Glu Glu Gly Thr Lys Lys Phe Val His Lys 
                725                 730                 735     
Gly Lys Val Glu Trp Gln Lys Gln Gly Lys Ser Lys Ile Lys Tyr Pro 
            740                 745                 750         
Val Ala Glu Trp Phe Leu Asp Val Tyr Ala Lys Glu Phe Leu Ser Leu 
        755                 760                 765             
Pro Glu Ala Pro Ser Trp Ser His Glu Glu Val Thr Glu Ile Leu Lys 
    770                 775                 780                 
Ser Arg Ala Arg Val Val Arg Ser Gln Leu Asn Glu Phe Asp Glu Tyr 
785                 790                 795                 800 
Phe Asp Asn Phe Ile Asp Trp Leu Arg Ser Glu Asn Ala Arg Gly Ile 
                805                 810                 815     
Tyr Pro Tyr Glu Lys Ala Asp Ser Ser His Ile Phe Ile Lys Gly Asn 
            820                 825                 830         
Met Ile Gly Ile Pro Pro Arg Leu Ala Glu Asp Phe Tyr Arg Asn Glu 
        835                 840                 845             
Leu Gly Ile Ser Gly Arg Lys Phe Lys Glu Met Leu Ile Arg Glu Leu 
    850                 855                 860                 
Gly Ser Tyr Tyr Leu Gly Lys Lys Ala Ala Trp Ile Lys Leu Ser Ser 
865                 870                 875                 880 
Gly Gln His Asn Gly Val Asn Cys Tyr Phe Ile Ser Leu Asp Trp Phe 
                885                 890                 895     
Lys Lys Ile Val Gly Glu Pro Asn Ile Lys Asp Ile Glu Ala Glu Gly 
            900                 905                 910         
Asp Ile Gly Ser Gly Gly Phe Asn Tyr Glu Glu Glu Glu Gly Glu Ala 
        915                 920                 925             



<210> 5
<211> 303
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpTCIR10Δ303-928

<400> 5
Met Ser Gly Arg Glu Phe Lys Arg Pro Ser Asp Val Ile Ile Asp Ile 
1               5                   10                  15      
Tyr Lys Val Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu 
            20                  25                  30          
Phe Arg Phe Tyr Pro Tyr Pro Thr Ser Glu Trp Ile Leu Leu Asn Asp 
        35                  40                  45              
Ile Glu Asp Lys Ala Arg Glu Ile Asp Lys Val Leu Phe Lys Asn Asn 
    50                  55                  60                  
Ile Leu Gly Lys Lys Glu Ala Tyr Ile Ser Met Ala Ile His Asp Phe 
65                  70                  75                  80  
Asp Glu Val Thr Lys Lys Leu Glu Lys Leu Gln Glu Leu Glu His Glu 
                85                  90                  95      
Lys Ala Gln Lys Glu Gly Arg Gln Pro Lys Glu Ile Thr Leu Arg His 
            100                 105                 110         
Val Gln Gly Glu Ala Thr Gly Lys Ile His Thr Thr Val Ser Ser Tyr 
        115                 120                 125             
Thr Leu Thr Leu Val Val Asp Ile Asp Val Asn Glu Ile His Asp Ser 
    130                 135                 140                 
Lys Ala Val Glu Ser Glu Glu Lys Ala Leu Glu Val Ser Lys Arg Ala 
145                 150                 155                 160 
Trp Glu Val Leu Lys Pro Asn Leu Glu Glu Leu Gly Ile Lys Pro Arg 
                165                 170                 175     
Tyr Val Phe Phe Thr Gly Gly Gly Ile Gln Leu Trp Phe Val Ala Pro 
            180                 185                 190         
Glu Pro Glu Asn Ile Ser Val Ile Asp Lys Ala Ala Glu Ile Ile Pro 
        195                 200                 205             
Pro Val Leu Asn Thr Leu Leu Pro Glu Gly Tyr Ser Val Asp Asn Ile 
    210                 215                 220                 
Phe Asp Arg Ala Arg Ile Val Arg Val Pro Phe Thr Val Asn Tyr Lys 
225                 230                 235                 240 
Tyr Lys Thr Pro Asp Gly Lys Pro Leu Glu Leu Arg Gly Arg Leu Leu 
                245                 250                 255     
Glu Phe Asn Asp Val Arg Thr Pro Leu Gly Asp Ile Leu Glu Lys Leu 
            260                 265                 270         
Glu Ala Tyr Ala Lys Gly His Lys Ile Ser Leu Gly Ser Thr Ser Arg 
        275                 280                 285             
Ser Gly Lys Phe Arg Gly Val Ala Gly Arg Tyr Glu Val Lys Lys 
    290                 295                 300             

<210> 6
<211> 914
<212> PRT
<213> Thermococcus peptonophilus


<220> 
<223> Thermococcus peptonophilus DNA primase

<400> 6
Met Ser Glu Leu Thr Pro Gly Lys Val Leu Ala Asp Val Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Tyr Pro Val Ile Lys Ser Glu Trp Val Leu Leu Asn Asp Ile Glu Asp 
        35                  40                  45              
Lys Ala Arg Asp Ile Asp Lys Val Leu Ala Lys Gln Asn Leu Ile Lys 
    50                  55                  60                  
Gly Lys Glu Ala Tyr Val Ser Met Ala Ile His Gly Phe Glu Ala Val 
65                  70                  75                  80  
Lys Lys Lys Leu Glu Lys Leu Arg Glu Ser Val Glu Glu Gly Lys Val 
                85                  90                  95      
Arg Lys Leu Gly Leu Glu Asn Val Gln Gly Glu Ala Lys Gly Lys Val 
            100                 105                 110         
His Pro Thr Val Ser Asn Tyr Thr Leu Thr Leu Val Val Asp Val Asp 
        115                 120                 125             
Ile Glu Ala Val His Lys Leu Lys Val Val Glu Asp Val Asp Lys Val 
    130                 135                 140                 
Phe Glu Lys Ala Lys Glu Gly Trp Leu Ala Leu Lys Pro Val Phe Glu 
145                 150                 155                 160 
Glu Leu Gly Val Leu Pro Arg Tyr Val Phe Phe Thr Gly Gly Gly Leu 
                165                 170                 175     
Gln Leu Trp Phe Val Ala Pro Lys Leu Glu Asp Ile Ala Val Ile Asp 
            180                 185                 190         
Arg Ala Ser Gly Ile Val Pro Asn Val Leu Asn Ala Leu Leu Pro Glu 
        195                 200                 205             
Gly Phe Val Val Asp Asn Ile Phe Asp Arg Ala Arg Ile Val Arg Ala 
    210                 215                 220                 
Pro Leu Thr Val Asn His Lys Tyr Lys Ala Pro Asn Gly Ala Arg Val 
225                 230                 235                 240 
Gly Val Lys Gly Arg Leu Ile Glu Phe Asn Asp Val Arg Val Ser Leu 
                245                 250                 255     
Ser Glu Val Leu Asp Lys Leu Glu Val Tyr Ala Lys Glu Arg Gly Ile 
            260                 265                 270         
Gln Leu Gly Gly Gln Glu Lys Val Arg Gly Gly Arg Gly Phe Val Asn 
        275                 280                 285             
Val Arg Tyr Val Val Lys Lys Glu Glu Leu Glu Thr Leu Ala Leu Asn 
    290                 295                 300                 
Leu Ala Asp Glu Leu Ile Pro Trp Phe Lys Lys Val Lys Glu Arg Gly 
305                 310                 315                 320 
Gly Ser Trp His His Leu Val Asn Ala Ile Gly Ala Tyr Val Val Arg 
                325                 330                 335     
Asn Thr Asn Leu Ser Leu Glu Asp Leu Ile Gly Lys Asp Asn Pro Asp 
            340                 345                 350         
Gly Thr His Val Val Gly Leu Trp Glu Ile Val Phe Gln Arg Leu Val 
        355                 360                 365             
Glu Lys Ser Ala Glu Asp Pro Gly Asp Trp Val Asn Arg Arg Asn Thr 
    370                 375                 380                 
Ile Lys Asp Val Tyr Glu Lys His Ile Ala Gly Lys Pro Leu Gly Thr 
385                 390                 395                 400 
Arg Ala Tyr Leu Lys Lys Tyr Leu Pro Val Ser Asp Glu Glu Val Val 
                405                 410                 415     
Glu Ile Leu Met Ala Val Arg Arg Ala Leu Leu Pro Phe Leu Lys Glu 
            420                 425                 430         
Val Lys Lys Val Asp Ser Ser Gly Phe Gly Val Ala Pro Tyr Lys Lys 
        435                 440                 445             
Thr Ala Pro Arg Ser Trp Asp Glu Val Asp Glu Asp Arg Lys Arg Ala 
    450                 455                 460                 
Thr Gly Arg Trp Tyr Val Trp Lys Leu Ala Phe Asn Thr Ala Glu Tyr 
465                 470                 475                 480 
Leu Phe Thr Asp Glu Leu Pro Lys Ala Gly Thr Phe Tyr Ile Asp Val 
                485                 490                 495     
Trp Met Lys Glu Gly Lys Arg Glu Trp Met Lys Arg Phe Phe Asn Glu 
            500                 505                 510         
Ala Leu Phe Arg Ser Phe Ile Glu Asp Gly Leu Gly Tyr Lys Tyr Gly 
        515                 520                 525             
Ala Pro Val Glu Arg Glu Glu Leu Phe Glu Arg Leu Val Glu Val Phe 
    530                 535                 540                 
Asn Ile Thr Asp Glu Glu Val Arg Gly Ile Tyr Ile Asp Ala Ala Leu 
545                 550                 555                 560 
Ser Leu Leu Ser Pro Val Gly Met Arg Thr Pro Pro Cys Ile Glu Glu 
                565                 570                 575     
Phe Ile Met Glu Phe Ala Ala Asn Gly Ser Leu Ser Glu Asp Lys Val 
            580                 585                 590         
Arg His Leu Ala Arg Trp Ile Lys Leu Tyr Ala Lys Pro Leu Lys His 
        595                 600                 605             
Ser Thr Thr Thr Thr Lys Leu Val Gly Ala Gly Tyr Lys Val Asp Met 
    610                 615                 620                 
Arg Met Ala Val Trp Ala Lys Leu Val Glu Phe Phe Ala Glu Asp Asp 
625                 630                 635                 640 
Glu Val Ala Arg Glu Leu Val Arg Val Phe Lys Glu Glu Tyr Gly Ala 
                645                 650                 655     
Ala Glu Pro Pro Phe Thr Cys Ile Gly Thr Lys Thr Cys Gln Phe Tyr 
            660                 665                 670         
Leu Asn Glu Lys Met Cys Pro Phe Ile Ile Pro Lys Glu Lys Glu Ile 
        675                 680                 685             
Leu Ala Val Ser Leu Ile Asp Val Gln Arg His Glu Ser Asp Gly Leu 
    690                 695                 700                 
Val Val Ile Val Gly Gly Asp Lys Glu Val Arg Thr Phe Val Lys Lys 
705                 710                 715                 720 
Gly Asn Val Glu Trp Val Lys Lys Thr Glu Arg Arg Glu Lys Tyr Pro 
                725                 730                 735     
Val Ala Glu Trp Phe Ile Asp Val Phe Ala Thr Glu Tyr Leu Ser Val 
            740                 745                 750         
Ser Pro Asp Asp Leu Asp Val Asp Leu Glu Glu Val Thr Asp Ile Leu 
        755                 760                 765             
Lys Ser Arg Ala Arg Val Val Lys Ser Arg Leu Asn Glu Leu Glu Asp 
    770                 775                 780                 
Met Tyr Glu Lys Phe Val Glu Trp Leu Lys Arg Glu Asn Ala Val Arg 
785                 790                 795                 800 
Gly Val Leu Pro Tyr Glu Lys Ala Asp Phe Asn His Leu Phe Ile Lys 
                805                 810                 815     
Gly Asn Met Ile Gly Ile Pro Pro Ala Leu Ala Glu Glu Phe Tyr Arg 
            820                 825                 830         
Phe Glu Leu Asn Ile Lys Gly Ser Glu Phe Arg Glu Met Leu Glu Lys 
        835                 840                 845             
Lys Leu Gly Phe His Tyr Thr Lys Lys Ala Val Lys Leu Ser Val Gly 
    850                 855                 860                 
Glu Lys Lys Asp Val Arg Arg Cys Tyr Leu Val Ser Leu Glu Trp Phe 
865                 870                 875                 880 
Arg Lys Val Val Gly Glu Pro Asn Val Lys Asp Val Val Met Ala Gly 
                885                 890                 895     
Asp Ile Ala Leu Ser Gly Leu Val Tyr Tyr Glu Ser Gly Glu Glu Val 
            900                 905                 910         
Val Glu 
        

<210> 7
<211> 295
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpTpepΔ295-914

<400> 7
Met Ser Glu Leu Thr Pro Gly Lys Val Leu Ala Asp Val Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Tyr Pro Val Ile Lys Ser Glu Trp Val Leu Leu Asn Asp Ile Glu Asp 
        35                  40                  45              
Lys Ala Arg Asp Ile Asp Lys Val Leu Ala Lys Gln Asn Leu Ile Lys 
    50                  55                  60                  
Gly Lys Glu Ala Tyr Val Ser Met Ala Ile His Gly Phe Glu Ala Val 
65                  70                  75                  80  
Lys Lys Lys Leu Glu Lys Leu Arg Glu Ser Val Glu Glu Gly Lys Val 
                85                  90                  95      
Arg Lys Leu Gly Leu Glu Asn Val Gln Gly Glu Ala Lys Gly Lys Val 
            100                 105                 110         
His Pro Thr Val Ser Asn Tyr Thr Leu Thr Leu Val Val Asp Val Asp 
        115                 120                 125             
Ile Glu Ala Val His Lys Leu Lys Val Val Glu Asp Val Asp Lys Val 
    130                 135                 140                 
Phe Glu Lys Ala Lys Glu Gly Trp Leu Ala Leu Lys Pro Val Phe Glu 
145                 150                 155                 160 
Glu Leu Gly Val Leu Pro Arg Tyr Val Phe Phe Thr Gly Gly Gly Leu 
                165                 170                 175     
Gln Leu Trp Phe Val Ala Pro Lys Leu Glu Asp Ile Ala Val Ile Asp 
            180                 185                 190         
Arg Ala Ser Gly Ile Val Pro Asn Val Leu Asn Ala Leu Leu Pro Glu 
        195                 200                 205             
Gly Phe Val Val Asp Asn Ile Phe Asp Arg Ala Arg Ile Val Arg Ala 
    210                 215                 220                 
Pro Leu Thr Val Asn His Lys Tyr Lys Ala Pro Asn Gly Ala Arg Val 
225                 230                 235                 240 
Gly Val Lys Gly Arg Leu Ile Glu Phe Asn Asp Val Arg Val Ser Leu 
                245                 250                 255     
Ser Glu Val Leu Asp Lys Leu Glu Val Tyr Ala Lys Glu Arg Gly Ile 
            260                 265                 270         
Gln Leu Gly Gly Gln Glu Lys Val Arg Gly Gly Arg Gly Phe Val Asn 
        275                 280                 285             
Val Arg Tyr Val Val Lys Lys 
    290                 295 

<210> 8
<211> 913
<212> PRT
<213> Thermococcus celericrescens


<220> 
<223> Thermococcus celericrescens DNA primase

<400> 8
Met Ser Glu Leu Thr Pro Gly Lys Val Leu Ala Asp Val Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Tyr Pro Val Ile Lys Ser Glu Trp Val Leu Leu Asn Asp Ile Glu Asp 
        35                  40                  45              
Lys Ala Arg Asp Ile Asp Lys Val Leu Ala Lys Lys Asn Ile Ile Asn 
    50                  55                  60                  
Gly Lys Glu Ala Tyr Val Ser Met Ala Ile His Asp Phe Gly Ala Val 
65                  70                  75                  80  
Lys Lys Lys Leu Glu Lys Leu Arg Glu Lys Ala Glu Gly Glu Arg Ala 
                85                  90                  95      
Arg Arg Ile Gly Leu Glu Asn Val Gln Gly Glu Ala Lys Gly Lys Val 
            100                 105                 110         
His Pro Thr Val Ser Asn Tyr Thr Leu Ala Leu Val Val Asp Ile Asp 
        115                 120                 125             
Ile Glu Glu Val His Lys Ser Arg Val Val Glu Asp Val Glu Ala Val 
    130                 135                 140                 
Phe Glu Arg Ala Lys Lys Gly Trp Leu Ala Leu Arg Pro Val Phe Glu 
145                 150                 155                 160 
Glu Leu Gly Val Leu Pro Arg Tyr Val Phe Phe Thr Gly Gly Gly Leu 
                165                 170                 175     
Gln Ile Trp Phe Val Ala Pro Glu Leu Glu Asp Ile Ala Val Ile Asp 
            180                 185                 190         
Arg Ala Ser Gly Ile Val Pro Gly Val Leu Asn Ala Leu Leu Pro Glu 
        195                 200                 205             
Gly Phe Val Val Asp Asn Ile Phe Asp Arg Ala Arg Ile Val Arg Ala 
    210                 215                 220                 
Pro Leu Thr Val Asn His Lys Tyr Lys Ala Pro Asn Gly Ala Gly Leu 
225                 230                 235                 240 
Gly Val Lys Gly Arg Leu Ile Glu Phe Asn Asp Val Arg Val Ser Leu 
                245                 250                 255     
Ser Glu Val Leu Asp Lys Leu Glu Ala Tyr Ala Lys Glu Lys Gly Ile 
            260                 265                 270         
Gln Leu Gly Gly Gln Glu Arg Ala Ser Gly Val Arg Val Phe Gly Lys 
        275                 280                 285             
Val Arg Tyr Glu Val Lys Lys Glu Arg Leu Glu Thr Leu Ala Leu Asn 
    290                 295                 300                 
Leu Ala Asp Glu Leu Ala Pro Trp Phe Lys Lys Val Lys Glu Arg Gly 
305                 310                 315                 320 
Gly Ser Trp His His Leu Val Asn Ala Ile Gly Ala Tyr Ile Val Arg 
                325                 330                 335     
Asn Thr Asn Leu Ser Leu Glu Asp Leu Ile Gly Lys Asp Asn Pro Asp 
            340                 345                 350         
Gly Thr His Val Val Gly Leu Trp Glu Leu Val Phe Gln Arg Leu Val 
        355                 360                 365             
Glu Lys Gly Ala Glu Asp Pro Ser Asp Trp Leu Asn Arg Arg Asn Thr 
    370                 375                 380                 
Ile Lys Asp Val Tyr Glu Lys His Ile Ala Gly Lys Pro Leu Gly Thr 
385                 390                 395                 400 
Arg Ala Tyr Leu Lys Lys Tyr Leu Pro Val Ser Asp Glu Glu Val Val 
                405                 410                 415     
Glu Ile Leu Met Ala Ala Arg Arg Ala Leu Leu Pro Phe Leu Lys Gly 
            420                 425                 430         
Val Lys Arg Ala Gly Ser Ser Gly Phe Gly Val Phe Pro Tyr Lys Asp 
        435                 440                 445             
Thr Ala Pro Arg Ser Trp Asn Glu Val Glu Ala Glu Arg Lys Arg Ala 
    450                 455                 460                 
Thr Gly Leu Trp Tyr Val Trp Ser Leu Ala Phe Ser Thr Ala Glu Tyr 
465                 470                 475                 480 
Ile Phe Thr Asp Glu Leu Ser Lys Ala Gly Thr Phe Phe Ile Leu Val 
                485                 490                 495     
Lys Glu Gly Lys Ser Ile Lys Ser Val Phe Asn Glu Thr Leu Phe Arg 
            500                 505                 510         
Ser Phe Ile Glu Glu Gly Leu Gly Tyr Lys Tyr Gly Met Pro Val Arg 
        515                 520                 525             
Arg Asp Glu Leu Phe Glu Arg Leu Val Glu Val Phe Asn Ile Thr Asp 
    530                 535                 540                 
Glu Glu Val Arg Gly Val Tyr Ile Asp Ala Ala Leu Ser Leu Leu Ser 
545                 550                 555                 560 
Pro Val Gly Met Arg Thr Pro Pro Cys Ile Glu Glu Phe Ile Met Glu 
                565                 570                 575     
Phe Ala Ala Asn Gly Asn Leu Pro Glu Asp Lys Val Arg His Leu Ala 
            580                 585                 590         
Arg Trp Ile Lys Leu Tyr Ala Lys Pro Leu Lys His Ser Thr Thr Thr 
        595                 600                 605             
Thr Lys Leu Val Gly Ala Gly Tyr Asn Val Asp Met Arg Met Ala Val 
    610                 615                 620                 
Trp Ala Lys Leu Val Glu Phe Phe Ala Glu Asp Asp Glu Val Ala Gly 
625                 630                 635                 640 
Glu Leu Val Arg Ile Phe Lys Glu Glu Tyr Lys Glu Ala Glu Pro Pro 
                645                 650                 655     
Phe Thr Cys Ile Gly Ala Arg Thr Cys Pro Phe Tyr Leu Lys Asp Asp 
            660                 665                 670         
Val Ala Lys Met Cys Pro Phe Ile Phe Pro Lys Glu Lys Glu Ile Leu 
        675                 680                 685             
Ala Val Ser Leu Ile Asp Val Gln Arg His Glu Ser Asp Gly Ile Val 
    690                 695                 700                 
Ile Leu Val Gly Gly Ala Arg Glu Val Arg Thr Phe Ile Lys Lys Gly 
705                 710                 715                 720 
Lys Val Glu Trp Val Lys Arg Thr Lys Lys Thr Glu Lys Tyr Pro Ile 
                725                 730                 735     
Ala Glu Trp Phe Ile Asp Val Phe Ala Thr Glu Tyr Leu Gly Val Pro 
            740                 745                 750         
Pro Asp Asn Leu Asp Phe Asp Leu Lys Asp Val Thr Gly Ile Leu Lys 
        755                 760                 765             
Ser Arg Glu Arg Val Val Pro Ser Gln Leu Asn Glu Val Glu Asp Trp 
    770                 775                 780                 
Tyr Glu Lys Phe Val Glu Trp Leu Lys Arg Glu Asn Glu Val Arg Gly 
785                 790                 795                 800 
Val Leu Pro Tyr Lys Lys Ala Asp Val His His Leu Phe Ile Lys Asp 
                805                 810                 815     
Asn Met Ile Gly Ile Pro Pro Ala Leu Ala Glu Glu Phe Tyr Arg Tyr 
            820                 825                 830         
Glu Ala Asn Ile Lys Pro Ser Glu Phe Arg Glu Met Leu Glu Val Lys 
        835                 840                 845             
Leu Ser Ile His Tyr Lys Lys Lys Ala Val Lys Leu Ser Val Gly Glu 
    850                 855                 860                 
Lys Lys Asp Val Arg Arg Cys Tyr Leu Val Ser Leu Glu Trp Phe Arg 
865                 870                 875                 880 
Lys Val Val Gly Glu Pro Asn Val Lys Asp Val Val Met Ala Gly Asp 
                885                 890                 895     
Ile Ala Leu Ser Gly Leu Val Tyr Tyr Glu Ser Gly Glu Glu Val Val 
            900                 905                 910         
Glu 
    

<210> 9
<211> 295
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpTceleΔ295-913

<400> 9
Met Ser Glu Leu Thr Pro Gly Lys Val Leu Ala Asp Val Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Glu Ala Gly Arg Leu Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Tyr Pro Val Ile Lys Ser Glu Trp Val Leu Leu Asn Asp Ile Glu Asp 
        35                  40                  45              
Lys Ala Arg Asp Ile Asp Lys Val Leu Ala Lys Lys Asn Ile Ile Asn 
    50                  55                  60                  
Gly Lys Glu Ala Tyr Val Ser Met Ala Ile His Asp Phe Gly Ala Val 
65                  70                  75                  80  
Lys Lys Lys Leu Glu Lys Leu Arg Glu Lys Ala Glu Gly Glu Arg Ala 
                85                  90                  95      
Arg Arg Ile Gly Leu Glu Asn Val Gln Gly Glu Ala Lys Gly Lys Val 
            100                 105                 110         
His Pro Thr Val Ser Asn Tyr Thr Leu Ala Leu Val Val Asp Ile Asp 
        115                 120                 125             
Ile Glu Glu Val His Lys Ser Arg Val Val Glu Asp Val Glu Ala Val 
    130                 135                 140                 
Phe Glu Arg Ala Lys Lys Gly Trp Leu Ala Leu Arg Pro Val Phe Glu 
145                 150                 155                 160 
Glu Leu Gly Val Leu Pro Arg Tyr Val Phe Phe Thr Gly Gly Gly Leu 
                165                 170                 175     
Gln Ile Trp Phe Val Ala Pro Glu Leu Glu Asp Ile Ala Val Ile Asp 
            180                 185                 190         
Arg Ala Ser Gly Ile Val Pro Gly Val Leu Asn Ala Leu Leu Pro Glu 
        195                 200                 205             
Gly Phe Val Val Asp Asn Ile Phe Asp Arg Ala Arg Ile Val Arg Ala 
    210                 215                 220                 
Pro Leu Thr Val Asn His Lys Tyr Lys Ala Pro Asn Gly Ala Gly Leu 
225                 230                 235                 240 
Gly Val Lys Gly Arg Leu Ile Glu Phe Asn Asp Val Arg Val Ser Leu 
                245                 250                 255     
Ser Glu Val Leu Asp Lys Leu Glu Ala Tyr Ala Lys Glu Lys Gly Ile 
            260                 265                 270         
Gln Leu Gly Gly Gln Glu Arg Ala Ser Gly Val Arg Val Phe Gly Lys 
        275                 280                 285             
Val Arg Tyr Glu Val Lys Lys 
    290                 295 

<210> 10
<211> 923
<212> PRT
<213> Thermococcus nautili


<220> 
<223> Thermococcus nautili sp. 30-1 DNA primase

<400> 10
Met Ser Ser Leu Arg Pro Ser Ser Ile Ile Ile Asp Ile Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Asp Ser Ser Arg Trp Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Leu Pro Lys Pro Ile Ser Ser Glu Trp Ile Phe Ile Thr Asp Ile Glu 
        35                  40                  45              
Glu Arg Ala Ser Glu Ile Asp Lys Val Leu Thr Lys Tyr Asn Ile Met 
    50                  55                  60                  
Lys Lys Lys Asp Ala Tyr Val Ser Met Ala Ile His Asp Phe Glu Lys 
65                  70                  75                  80  
Val Thr Ala Lys Leu Lys Arg Val Gln Glu Glu Glu Asn Lys Lys Ala 
                85                  90                  95      
Thr Glu Gly Glu Arg Arg Leu Arg Arg Ile Thr Leu Asp Lys Ile Gln 
            100                 105                 110         
Gly Asp Ser Glu Thr Thr Ser Gly Phe Thr Leu Ala Leu Val Val Asp 
        115                 120                 125             
Ile Asp Asn Thr Lys Ile His Asp Thr Arg Ile Ile Glu Asn Glu Glu 
    130                 135                 140                 
Glu Ala Phe Glu Ala Ser Lys Arg Glu Trp Glu Ala Leu Lys Pro Lys 
145                 150                 155                 160 
Leu Gln Glu Leu Gly Phe Leu Pro Arg Trp Ile Leu Tyr Thr Gly Gly 
                165                 170                 175     
Gly Leu Gln Leu Trp Phe Val Ser Asp Lys Leu Glu Pro Ile Ser Val 
            180                 185                 190         
Ile Asp Arg Ala Ser Glu Ile Ile Pro Asn Ile Met Asn Gly Val Asn 
        195                 200                 205             
Gly Val Lys Gly Leu Leu Ser Glu Gly Phe Lys Ala Asp Asn Ile Phe 
    210                 215                 220                 
Asp Pro Ala Arg Ile Val Arg Ala Pro Leu Thr Phe Asn His Lys Tyr 
225                 230                 235                 240 
Arg Thr Ile Ile Lys Asp Glu Asp Gly Thr Glu Arg Val Val Pro Thr 
                245                 250                 255     
Gln Val Lys Gly Arg Val Ile Glu Phe Asn Asp Val Arg Ile Ser Leu 
            260                 265                 270         
Thr Glu Phe Leu Asp Arg Leu Glu Ala Tyr Ala Lys Glu Lys Gly Ile 
        275                 280                 285             
Pro Leu Glu Lys Pro Thr Lys Arg Lys Phe Leu Glu Leu Ala Ser Lys 
    290                 295                 300                 
Arg Tyr Glu Val Thr Ser Ser Asn Phe Glu Ala Leu Ala Glu Arg Leu 
305                 310                 315                 320 
Phe Thr Glu Leu Arg Pro Trp Trp Glu Ile Ala Lys Glu Lys Gly Trp 
                325                 330                 335     
Ser Arg His His Leu Thr Met Gly Ile Ala Thr Tyr Ile Leu Arg Asn 
            340                 345                 350         
Thr Asn Leu Thr Pro Glu Gln Leu Ile Gly Ser Glu Asn Ser Pro Gly 
        355                 360                 365             
Leu Trp Glu Leu Val Phe Ala Lys Leu Val Glu Ala Gly Leu Glu Asp 
    370                 375                 380                 
Pro Asp Asp Trp Lys Asn Arg Ala Ser Thr Ile Arg Asp Ala Tyr Lys 
385                 390                 395                 400 
Lys Ile Glu Ser Gly Lys Lys Val Ala Thr Lys Ala Tyr Leu Arg Lys 
                405                 410                 415     
Tyr Ile Glu Gly Leu Ser Glu Glu Asp Ala Val Gln Ile Leu Leu Ser 
            420                 425                 430         
Val Lys Arg Ala Leu Leu Pro Tyr Leu Lys Ala Val Asp Val Lys Arg 
        435                 440                 445             
Ile Ser Lys Tyr Ser Ala Arg Pro Tyr Glu Ile Thr Glu Glu Ile Pro 
    450                 455                 460                 
Lys Ser Trp Glu Asp Ile Asp Glu Asn Arg Lys Lys Ala Thr Gly Val 
465                 470                 475                 480 
Trp Tyr Ile Asp Phe Leu Ala Leu Glu Thr Ala Asn Asp Ile Tyr Phe 
                485                 490                 495     
Glu Asp Leu Pro Lys Pro Pro Val Phe Tyr Ile Arg Phe Val Glu Lys 
            500                 505                 510         
Asn Lys Glu Lys Phe Lys Leu Asn Glu Thr Leu Tyr His Ser Phe Leu 
        515                 520                 525             
Thr Trp Leu Gly Ile Thr Glu Gly Glu Pro Leu Asp Arg Thr Glu Leu 
    530                 535                 540                 
Ile Asp Leu Leu Val Glu Lys Phe Gly Phe Thr Val Glu Asp Leu Lys 
545                 550                 555                 560 
Ala Ile Tyr Tyr Arg Lys Ile Leu Thr Leu Leu Lys Pro Glu Gly Met 
                565                 570                 575     
Arg Thr Pro Lys Cys Ile Gln Glu Phe Leu Phe Glu Leu Ala Thr Glu 
            580                 585                 590         
Gly Asn Leu Pro Glu Asp Lys Ile Arg His Leu Ala His Trp Val Lys 
        595                 600                 605             
Phe Tyr Ala Arg Pro Leu Arg His Ser Thr Thr Ser Val Leu Leu Lys 
    610                 615                 620                 
Gly Arg Gly Lys Pro Val Asp Met Arg Leu Ala Ile Trp Ala Lys Val 
625                 630                 635                 640 
Val Glu Phe Phe Ala Glu Asp Asp Glu Val Ala Glu Glu Leu Ile Thr 
                645                 650                 655     
Thr Phe Lys Lys Ala Tyr Met Gly Ala Glu Pro Pro Phe Pro Cys Ile 
            660                 665                 670         
Gly Ala Glu Ser Cys Pro Phe Tyr Pro Asp His Arg Ala Cys Pro Phe 
        675                 680                 685             
Ile Val Pro Lys Arg Lys Glu Val Leu Pro Val Ser Ile Val Asp Val 
    690                 695                 700                 
Gln Leu His Gly Ser Asp Gly Ile Val Val Leu Val Gly Gly Pro Thr 
705                 710                 715                 720 
Glu Val Thr Ala Phe Thr Leu Glu Gly Lys Val Glu Trp Ile Lys Thr 
                725                 730                 735     
Thr Lys Lys Thr Val Lys Tyr Pro Ile Ala Glu Trp Phe Leu Asp Arg 
            740                 745                 750         
Phe Ala Lys Glu Tyr Leu Ser Leu Pro Glu Ala Pro Ser Trp Trp Lys 
        755                 760                 765             
Leu Glu Glu Val Thr Glu Ile Leu Lys Ser Arg Ala Arg Val Val Lys 
    770                 775                 780                 
Ser Gln Phe Asp Lys Phe Glu Asp Tyr Leu Glu Gln Phe Ile Glu Trp 
785                 790                 795                 800 
Leu Gln Lys Glu Asn Ser Arg Arg Gly Ile Leu Pro Tyr Glu Lys Ala 
                805                 810                 815     
Asp Glu Asn His Leu Phe Ile Lys Gly Glu Trp Val Gly Ile Pro Pro 
            820                 825                 830         
Gly Phe Ala Arg Glu Phe Tyr Ser Gly Glu Leu Leu Ile Gly Gly Pro 
        835                 840                 845             
Thr Phe Arg Arg Met Leu Glu Gln Lys Leu Gly Lys Asp Tyr Arg Lys 
    850                 855                 860                 
Met Ser Ala Lys Ile His Leu Asn Thr Gly Leu Lys Asp Lys Arg Asn 
865                 870                 875                 880 
Cys Tyr Phe Val Ser Val Glu Trp Phe Arg Lys His Val Gly Glu Pro 
                885                 890                 895     
Asn Ile Gln Glu Ile Thr Ser Glu Gly Asp Val Ser Phe Asp Gly Leu 
            900                 905                 910         
Ser Tyr Asp Asp Glu Glu Glu Gly Val Val Gly 
        915                 920             

<210> 11
<211> 310
<212> PRT
<213> Artificial Sequence


<220> 
<223> PolpTN2Δ311-923

<400> 11
Met Ser Ser Leu Arg Pro Ser Ser Ile Ile Ile Asp Ile Tyr Lys Val 
1               5                   10                  15      
Ile Gln Asp His Pro Asp Ser Ser Arg Trp Ala Ile Glu Leu Arg Phe 
            20                  25                  30          
Leu Pro Lys Pro Ile Ser Ser Glu Trp Ile Phe Ile Thr Asp Ile Glu 
        35                  40                  45              
Glu Arg Ala Ser Glu Ile Asp Lys Val Leu Thr Lys Tyr Asn Ile Met 
    50                  55                  60                  
Lys Lys Lys Asp Ala Tyr Val Ser Met Ala Ile His Asp Phe Glu Lys 
65                  70                  75                  80  
Val Thr Ala Lys Leu Lys Arg Val Gln Glu Glu Glu Asn Lys Lys Ala 
                85                  90                  95      
Thr Glu Gly Glu Arg Arg Leu Arg Arg Ile Thr Leu Asp Lys Ile Gln 
            100                 105                 110         
Gly Asp Ser Glu Thr Thr Ser Gly Phe Thr Leu Ala Leu Val Val Asp 
        115                 120                 125             
Ile Asp Asn Thr Lys Ile His Asp Thr Arg Ile Ile Glu Asn Glu Glu 
    130                 135                 140                 
Glu Ala Phe Glu Ala Ser Lys Arg Glu Trp Glu Ala Leu Lys Pro Lys 
145                 150                 155                 160 
Leu Gln Glu Leu Gly Phe Leu Pro Arg Trp Ile Leu Tyr Thr Gly Gly 
                165                 170                 175     
Gly Leu Gln Leu Trp Phe Val Ser Asp Lys Leu Glu Pro Ile Ser Val 
            180                 185                 190         
Ile Asp Arg Ala Ser Glu Ile Ile Pro Asn Ile Met Asn Gly Val Asn 
        195                 200                 205             
Gly Val Lys Gly Leu Leu Ser Glu Gly Phe Lys Ala Asp Asn Ile Phe 
    210                 215                 220                 
Asp Pro Ala Arg Ile Val Arg Ala Pro Leu Thr Phe Asn His Lys Tyr 
225                 230                 235                 240 
Arg Thr Ile Ile Lys Asp Glu Asp Gly Thr Glu Arg Val Val Pro Thr 
                245                 250                 255     
Gln Val Lys Gly Arg Val Ile Glu Phe Asn Asp Val Arg Ile Ser Leu 
            260                 265                 270         
Thr Glu Phe Leu Asp Arg Leu Glu Ala Tyr Ala Lys Glu Lys Gly Ile 
        275                 280                 285             
Pro Leu Glu Lys Pro Thr Lys Arg Lys Phe Leu Glu Leu Ala Ser Lys 
    290                 295                 300                 
Arg Tyr Glu Val Thr Ser 
305                 310 

<210> 12
<211> 347
<212> PRT
<213> Pyrococcus furiosus


<220> 
<223> DNA primase catalytic subunit PriS

<400> 12
Met Leu Met Arg Glu Val Thr Lys Glu Glu Arg Ser Glu Phe Tyr Ser 
1               5                   10                  15      
Lys Glu Trp Ser Ala Lys Lys Ile Pro Lys Phe Ile Val Asp Thr Leu 
            20                  25                  30          
Glu Ser Arg Glu Phe Gly Phe Asp His Asn Gly Glu Gly Pro Ser Asp 
        35                  40                  45              
Arg Lys Asn Gln Tyr Ser Asp Ile Arg Asp Leu Glu Asp Tyr Ile Arg 
    50                  55                  60                  
Ala Thr Ser Pro Tyr Ala Val Tyr Ser Ser Val Ala Phe Tyr Glu Asn 
65                  70                  75                  80  
Pro Arg Glu Met Glu Gly Trp Arg Gly Ala Glu Leu Val Phe Asp Ile 
                85                  90                  95      
Asp Ala Lys Asp Leu Pro Leu Lys Arg Cys Asn His Glu Pro Gly Thr 
            100                 105                 110         
Val Cys Pro Ile Cys Leu Glu Asp Ala Lys Glu Leu Ala Lys Asp Thr 
        115                 120                 125             
Leu Ile Ile Leu Arg Glu Glu Leu Gly Phe Glu Asn Ile His Val Val 
    130                 135                 140                 
Tyr Ser Gly Arg Gly Tyr His Ile Arg Ile Leu Asp Glu Trp Ala Leu 
145                 150                 155                 160 
Gln Leu Asp Ser Lys Ser Arg Glu Arg Ile Leu Ala Phe Ile Ser Ala 
                165                 170                 175     
Ser Glu Ile Glu Asn Val Glu Glu Phe Arg Arg Phe Leu Leu Glu Lys 
            180                 185                 190         
Arg Gly Trp Phe Val Leu Lys His Gly Tyr Pro Arg Val Phe Arg Leu 
        195                 200                 205             
Arg Leu Gly Tyr Phe Ile Leu Arg Val Asn Val Pro His Leu Leu Ser 
    210                 215                 220                 
Ile Gly Ile Arg Arg Asn Ile Ala Lys Lys Ile Leu Asp His Lys Glu 
225                 230                 235                 240 
Glu Ile Tyr Glu Gly Phe Val Arg Lys Ala Ile Leu Ala Ser Phe Pro 
                245                 250                 255     
Glu Gly Val Gly Ile Glu Ser Met Ala Lys Leu Phe Ala Leu Ser Thr 
            260                 265                 270         
Arg Phe Ser Lys Ala Tyr Phe Asp Gly Arg Val Thr Val Asp Ile Lys 
        275                 280                 285             
Arg Ile Leu Arg Leu Pro Ser Thr Leu His Ser Lys Val Gly Leu Ile 
    290                 295                 300                 
Ala Thr Tyr Val Gly Thr Lys Glu Arg Glu Val Met Lys Phe Asn Pro 
305                 310                 315                 320 
Phe Arg His Ala Val Pro Lys Phe Arg Lys Lys Glu Val Arg Glu Ala 
                325                 330                 335     
Tyr Lys Leu Trp Arg Glu Ser Leu Glu Tyr Glu 
            340                 345         

<210> 13
<211> 346
<212> PRT
<213> Thermococcus kodakarensis


<220> 
<223> DNA primase catalytic subunit PriS

<400> 13
Met Ser Lys Leu Leu Arg Glu Val Thr Pro Glu Glu Arg Arg Leu Tyr 
1               5                   10                  15      
Tyr Ser Gly Glu Trp Asp Ala Lys Lys Leu Pro Glu Phe Ile Val Glu 
            20                  25                  30          
Ser Ile Glu Arg Arg Glu Phe Gly Phe Asp His Thr Gly Glu Gly Pro 
        35                  40                  45              
Ser Asp Arg Lys Asn Ala Phe Ser Asp Val Arg Asp Leu Glu Asp Tyr 
    50                  55                  60                  
Ile Arg Ala Thr Ala Pro Tyr Ala Ala Tyr Ser Ser Val Ala Phe Tyr 
65                  70                  75                  80  
Arg Asn Pro Gln Glu Met Glu Gly Trp Leu Gly Ala Glu Leu Val Phe 
                85                  90                  95      
Asp Ile Asp Ala Lys Asp Leu Pro Leu Arg Arg Cys Gln Asn Glu His 
            100                 105                 110         
Pro Ser Gly Gln Val Cys Pro Ile Cys Leu Glu Asp Ala Lys Glu Leu 
        115                 120                 125             
Ala Arg Asp Thr Leu Ile Ile Leu Lys Glu Asp Phe Gly Phe Glu Asn 
    130                 135                 140                 
Ile His Val Val Tyr Ser Gly Arg Gly Tyr His Ile Arg Val Ile Asp 
145                 150                 155                 160 
Glu Trp Ala Leu Lys Leu Asp Ser Lys Ala Arg Glu Arg Ile Leu Ser 
                165                 170                 175     
Tyr Val Ser Ala Ala Glu Glu Val Thr Phe Asp Asp Ile Gln Lys Arg 
            180                 185                 190         
Tyr Ile Met Leu Ser Ser Gly Tyr Phe Arg Val Phe Arg Leu Arg Phe 
        195                 200                 205             
Gly Tyr Phe Ile Gln Arg Ile Asn Glu Asn His Leu Lys Asn Ile Gly 
    210                 215                 220                 
Leu Lys Arg Ser Thr Ala Glu Lys Leu Leu Asp Glu Lys Thr Arg Gln 
225                 230                 235                 240 
Asp Ile Val Glu Lys Phe Val Asn Lys Gly Leu Leu Ala Ala Phe Pro 
                245                 250                 255     
Glu Gly Val Gly Tyr Arg Thr Leu Leu Arg Leu Phe Gly Leu Ser Thr 
            260                 265                 270         
Thr Phe Ser Lys Ala Tyr Phe Asp Gly Arg Val Thr Val Asp Leu Lys 
        275                 280                 285             
Arg Ile Leu Arg Leu Pro Ser Thr Leu His Ser Lys Val Gly Leu Val 
    290                 295                 300                 
Ala Thr Tyr Ile Gly Ser Asp Glu Lys Arg Leu Glu Lys Phe Asp Pro 
305                 310                 315                 320 
Phe Lys Asp Ala Val Pro Glu Phe Arg Lys Glu Glu Val Gln Lys Ala 
                325                 330                 335     
Tyr Gln Glu Trp Lys Glu Leu His Glu Gly 
            340                 345     

<210> 14
<211> 330
<212> PRT
<213> Sulfolobus solfataricus


<220> 
<223> DNA primase small subunit PriS

<400> 14
Met Gly Thr Phe Thr Leu His Gln Gly Gln Thr Asn Leu Ile Lys Ser 
1               5                   10                  15      
Phe Phe Arg Asn Tyr Tyr Leu Asn Ala Glu Leu Glu Leu Pro Lys Asp 
            20                  25                  30          
Met Glu Leu Arg Glu Phe Ala Leu Gln Pro Phe Gly Ser Asp Thr Tyr 
        35                  40                  45              
Val Arg His Leu Ser Phe Ser Ser Ser Glu Glu Leu Arg Asp Tyr Leu 
    50                  55                  60                  
Val Asn Arg Asn Leu Pro Leu His Leu Phe Tyr Ser Ser Ala Arg Tyr 
65                  70                  75                  80  
Gln Leu Pro Ser Ala Arg Asn Met Glu Glu Lys Ala Trp Met Gly Ser 
                85                  90                  95      
Asp Leu Leu Phe Asp Ile Asp Ala Asp His Leu Cys Lys Leu Arg Ser 
            100                 105                 110         
Ile Arg Phe Cys Pro Val Cys Gly Asn Ala Val Val Ser Glu Lys Cys 
        115                 120                 125             
Glu Arg Asp Asn Val Glu Thr Leu Glu Tyr Val Glu Met Thr Ser Glu 
    130                 135                 140                 
Cys Ile Lys Arg Gly Leu Glu Gln Thr Arg Asn Leu Val Glu Ile Leu 
145                 150                 155                 160 
Glu Asp Asp Phe Gly Leu Lys Pro Lys Val Tyr Phe Ser Gly Asn Arg 
                165                 170                 175     
Gly Phe His Val Gln Val Asp Cys Tyr Gly Asn Cys Ala Leu Leu Asp 
            180                 185                 190         
Ser Asp Glu Arg Lys Glu Ile Ala Glu Tyr Val Met Gly Ile Gly Val 
        195                 200                 205             
Pro Gly Tyr Pro Gly Gly Ser Glu Asn Ala Pro Gly Trp Val Gly Arg 
    210                 215                 220                 
Lys Asn Arg Gly Ile Asn Gly Val Thr Ile Asp Glu Gln Val Thr Ile 
225                 230                 235                 240 
Asp Val Lys Arg Leu Ile Arg Ile Pro Asn Ser Leu His Gly Lys Ser 
                245                 250                 255     
Gly Leu Ile Val Lys Arg Val Pro Asn Leu Asp Asp Phe Glu Phe Asn 
            260                 265                 270         
Glu Thr Leu Ser Pro Phe Thr Gly Tyr Thr Ile Phe Leu Pro Tyr Ile 
        275                 280                 285             
Thr Ile Glu Thr Glu Val Leu Gly Ser Ile Ile Lys Leu Asn Arg Gly 
    290                 295                 300                 
Ile Pro Ile Lys Ile Lys Ser Ser Ile Gly Ile Tyr Leu His Leu Arg 
305                 310                 315                 320 
Asn Leu Gly Glu Val Lys Ala Tyr Val Arg 
                325                 330 

<210> 15
<211> 346
<212> PRT
<213> Pyrococcus horikoshii


<220> 
<223> DNA primase small subunit PriS

<400> 15
Met Leu Leu Arg Glu Val Thr Arg Glu Glu Arg Lys Asn Phe Tyr Thr 
1               5                   10                  15      
Asn Glu Trp Lys Val Lys Asp Ile Pro Asp Phe Ile Val Lys Thr Leu 
            20                  25                  30          
Glu Leu Arg Glu Phe Gly Phe Asp His Ser Gly Glu Gly Pro Ser Asp 
        35                  40                  45              
Arg Lys Asn Gln Tyr Thr Asp Ile Arg Asp Leu Glu Asp Tyr Ile Arg 
    50                  55                  60                  
Ala Thr Ala Pro Tyr Ala Val Tyr Ser Ser Val Ala Leu Tyr Glu Lys 
65                  70                  75                  80  
Pro Gln Glu Met Glu Gly Trp Leu Gly Thr Glu Leu Val Phe Asp Ile 
                85                  90                  95      
Asp Ala Lys Asp Leu Pro Leu Arg Arg Cys Glu His Glu Pro Gly Thr 
            100                 105                 110         
Val Cys Pro Ile Cys Leu Asn Asp Ala Lys Glu Ile Val Arg Asp Thr 
        115                 120                 125             
Val Ile Ile Leu Arg Glu Glu Leu Gly Phe Asn Asp Ile His Ile Ile 
    130                 135                 140                 
Tyr Ser Gly Arg Gly Tyr His Ile Arg Val Leu Asp Glu Trp Ala Leu 
145                 150                 155                 160 
Lys Leu Asp Ser Lys Ser Arg Glu Arg Ile Leu Ser Phe Val Ser Ala 
                165                 170                 175     
Ser Glu Ile Glu Asp Val Glu Glu Phe Arg Lys Leu Leu Leu Asn Lys 
            180                 185                 190         
Arg Gly Trp Phe Val Leu Asn His Gly Tyr Pro Arg Ala Phe Arg Leu 
        195                 200                 205             
Arg Phe Gly Tyr Phe Ile Leu Arg Ile Lys Leu Pro His Leu Ile Asn 
    210                 215                 220                 
Ala Gly Ile Arg Lys Ser Ile Ala Lys Ser Ile Leu Lys Ser Lys Glu 
225                 230                 235                 240 
Glu Ile Tyr Glu Glu Phe Val Arg Lys Ala Ile Leu Ala Ala Phe Pro 
                245                 250                 255     
Gln Gly Val Gly Ile Glu Ser Leu Ala Lys Leu Phe Ala Leu Ser Thr 
            260                 265                 270         
Arg Phe Ser Lys Ser Tyr Phe Asp Gly Arg Val Thr Val Asp Leu Lys 
        275                 280                 285             
Arg Ile Leu Arg Leu Pro Ser Thr Leu His Ser Lys Val Gly Leu Ile 
    290                 295                 300                 
Ala Lys Tyr Val Gly Thr Asn Glu Arg Asp Val Met Arg Phe Asn Pro 
305                 310                 315                 320 
Phe Lys His Ala Val Pro Lys Phe Arg Lys Glu Glu Val Lys Val Glu 
                325                 330                 335     
Tyr Lys Lys Phe Leu Glu Ser Leu Gly Thr 
            340                 345     

<210> 16
<211> 335
<212> PRT
<213> Archaeoglobus fulgidus


<220> 
<223> DNA primase small subunit PriS

<400> 16
Met Leu Thr Lys Leu Phe Leu Lys Lys Lys Phe Glu Glu Tyr Tyr Ser 
1               5                   10                  15      
Lys Asn Glu Val Glu Leu Pro Arg Lys Phe Lys Asn Arg Glu Phe Ala 
            20                  25                  30          
Phe Val Pro Leu Glu Leu Leu Pro Asp Phe Val Met His Arg His Ile 
        35                  40                  45              
Ser Phe Arg Ser Glu Thr Asp Phe Arg Ala Tyr Ile Leu Ser Asn Val 
    50                  55                  60                  
Pro Ala His Ile Tyr Phe Ser Ser Ala Tyr Tyr Glu Arg Pro Ala Glu 
65                  70                  75                  80  
Asp Lys Met Glu Asn Lys Gly Trp Leu Gly Ala Asp Leu Ile Phe Asp 
                85                  90                  95      
Ile Asp Ala Asp His Leu Pro Val Lys Ala Gln Ser Phe Glu Lys Ala 
            100                 105                 110         
Leu Glu Met Ala Lys Arg Glu Ile Lys Lys Leu Thr Ala Val Leu Arg 
        115                 120                 125             
Ala Asp Phe Gly Ile Arg Asp Met Lys Ile Tyr Phe Ser Gly Gly Arg 
    130                 135                 140                 
Gly Tyr His Val His Val His Asp Glu Glu Phe Leu Ser Leu Gly Ser 
145                 150                 155                 160 
Ala Glu Arg Arg Glu Ile Val Asp Tyr Leu Arg Leu Asn Ser Pro Lys 
                165                 170                 175     
Ile Val Val Glu Asp Arg Phe Ala Asn Ser Asn Ala Ala Lys Arg Val 
            180                 185                 190         
Leu Asn Tyr Leu Arg Lys Lys Leu Glu Glu Asp Glu Arg Leu Thr Ser 
        195                 200                 205             
Lys Leu Lys Ile Lys Pro Ala Asp Leu Lys Lys Glu Lys Leu Thr Lys 
    210                 215                 220                 
Lys Val Ile Arg Ala Val Glu Lys Phe Asp Tyr Ser Ala Leu Ser Ile 
225                 230                 235                 240 
Tyr Ile Asp Ala Pro Val Thr Ala Asp Val Lys Arg Leu Ile Arg Leu 
                245                 250                 255     
Pro Gly Ser Leu His Gly Lys Thr Gly Leu Arg Val Thr Glu Val Glu 
            260                 265                 270         
Asp Ile Glu Ser Phe Asn Pro Leu Lys Asp Ala Leu Ala Phe Gly Asp 
        275                 280                 285             
Glu Ala Val Val Val Lys Val Ala Arg Lys Leu Asn Leu Ser Ile Gly 
    290                 295                 300                 
Asp Phe Ser Gly Lys Ile Tyr Pro Gly Arg Val Lys Leu Pro Glu Tyr 
305                 310                 315                 320 
Ala Ala Val Phe Leu Ile Cys Arg Gly Asp Ala Ser Tyr Asp Ser 
                325                 330                 335 

