                         SEQUENCE LISTING

<110>  FLAGSHIP PIONEERING INNOVATIONS VI, LLC
 
<120>  METHODS FOR MANUFACTURING ADAS

<130>  51296-042WO2

<150>  US 63/040,459
<151>  2020-06-17

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  88
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ala Leu Leu Asp Phe Phe Leu Ser Arg Lys Lys Asn Thr Ala Asn 
1               5                   10                  15      


Ile Ala Lys Glu Arg Leu Gln Ile Ile Val Ala Glu Arg Arg Arg Ser 
            20                  25                  30          


Asp Ala Glu Pro His Tyr Leu Pro Gln Leu Arg Lys Asp Ile Leu Glu 
        35                  40                  45              


Val Ile Cys Lys Tyr Val Gln Ile Asp Pro Glu Met Val Thr Val Gln 
    50                  55                  60                  


Leu Glu Gln Lys Asp Gly Asp Ile Ser Ile Leu Glu Leu Asn Val Thr 
65                  70                  75                  80  


Leu Pro Glu Ala Glu Glu Leu Lys 
                85              


<210>  2
<211>  231
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Ser Asn Thr Pro Ile Glu Leu Lys Gly Ser Ser Phe Thr Leu Ser 
1               5                   10                  15      


Val Val His Leu His Glu Ala Glu Pro Lys Val Ile His Gln Ala Leu 
            20                  25                  30          


Glu Asp Lys Ile Ala Gln Ala Pro Ala Phe Leu Lys His Ala Pro Val 
        35                  40                  45              


Val Leu Asn Val Ser Ala Leu Glu Asp Pro Val Asn Trp Ser Ala Met 
    50                  55                  60                  


His Lys Ala Val Ser Ala Thr Gly Leu Arg Val Ile Gly Val Ser Gly 
65                  70                  75                  80  


Cys Lys Asp Ala Gln Leu Lys Ala Glu Ile Glu Lys Met Gly Leu Pro 
                85                  90                  95      


Ile Leu Thr Glu Gly Lys Glu Lys Ala Pro Arg Pro Ala Pro Thr Pro 
            100                 105                 110         


Gln Ala Pro Ala Gln Asn Thr Thr Pro Val Thr Lys Thr Arg Leu Ile 
        115                 120                 125             


Asp Thr Pro Val Arg Ser Gly Gln Arg Ile Tyr Ala Pro Gln Cys Asp 
    130                 135                 140                 


Leu Ile Val Thr Ser His Val Ser Ala Gly Ala Glu Leu Ile Ala Asp 
145                 150                 155                 160 


Gly Asn Ile His Val Tyr Gly Met Met Arg Gly Arg Ala Leu Ala Gly 
                165                 170                 175     


Ala Ser Gly Asp Arg Glu Thr Gln Ile Phe Cys Thr Asn Leu Met Ala 
            180                 185                 190         


Glu Leu Val Ser Ile Ala Gly Glu Tyr Trp Leu Ser Asp Gln Ile Pro 
        195                 200                 205             


Ala Glu Phe Tyr Gly Lys Ala Ala Arg Leu Gln Leu Val Glu Asn Ala 
    210                 215                 220                 


Leu Thr Val Gln Pro Leu Asn 
225                 230     


<210>  3
<211>  270
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Ala Arg Ile Ile Val Val Thr Ser Gly Lys Gly Gly Val Gly Lys 
1               5                   10                  15      


Thr Thr Ser Ser Ala Ala Ile Ala Thr Gly Leu Ala Gln Lys Gly Lys 
            20                  25                  30          


Lys Thr Val Val Ile Asp Phe Asp Ile Gly Leu Arg Asn Leu Asp Leu 
        35                  40                  45              


Ile Met Gly Cys Glu Arg Arg Val Val Tyr Asp Phe Val Asn Val Ile 
    50                  55                  60                  


Gln Gly Asp Ala Thr Leu Asn Gln Ala Leu Ile Lys Asp Lys Arg Thr 
65                  70                  75                  80  


Glu Asn Leu Tyr Ile Leu Pro Ala Ser Gln Thr Arg Asp Lys Asp Ala 
                85                  90                  95      


Leu Thr Arg Glu Gly Val Ala Lys Val Leu Asp Asp Leu Lys Ala Met 
            100                 105                 110         


Asp Phe Glu Phe Ile Val Cys Asp Ser Pro Ala Gly Ile Glu Thr Gly 
        115                 120                 125             


Ala Leu Met Ala Leu Tyr Phe Ala Asp Glu Ala Ile Ile Thr Thr Asn 
    130                 135                 140                 


Pro Glu Val Ser Ser Val Arg Asp Ser Asp Arg Ile Leu Gly Ile Leu 
145                 150                 155                 160 


Ala Ser Lys Ser Arg Arg Ala Glu Asn Gly Glu Glu Pro Ile Lys Glu 
                165                 170                 175     


His Leu Leu Leu Thr Arg Tyr Asn Pro Gly Arg Val Ser Arg Gly Asp 
            180                 185                 190         


Met Leu Ser Met Glu Asp Val Leu Glu Ile Leu Arg Ile Lys Leu Val 
        195                 200                 205             


Gly Val Ile Pro Glu Asp Gln Ser Val Leu Arg Ala Ser Asn Gln Gly 
    210                 215                 220                 


Glu Pro Val Ile Leu Asp Ile Asn Ala Asp Ala Gly Lys Ala Tyr Ala 
225                 230                 235                 240 


Asp Thr Val Glu Arg Leu Leu Gly Glu Glu Arg Pro Phe Arg Phe Ile 
                245                 250                 255     


Glu Glu Glu Lys Lys Gly Phe Leu Lys Arg Leu Phe Gly Gly 
            260                 265                 270 


<210>  4
<211>  164
<212>  PRT
<213>  Bacillus subtilis

<400>  4

Met Pro Leu Thr Pro Asn Asp Ile His Asn Lys Thr Phe Thr Lys Ser 
1               5                   10                  15      


Phe Arg Gly Tyr Asp Glu Asp Glu Val Asn Glu Phe Leu Ala Gln Val 
            20                  25                  30          


Arg Lys Asp Tyr Glu Ile Val Leu Arg Lys Lys Thr Glu Leu Glu Ala 
        35                  40                  45              


Lys Val Asn Glu Leu Asp Glu Arg Ile Gly His Phe Ala Asn Ile Glu 
    50                  55                  60                  


Glu Thr Leu Asn Lys Ser Ile Leu Val Ala Gln Glu Ala Ala Glu Asp 
65                  70                  75                  80  


Val Lys Arg Asn Ser Gln Lys Glu Ala Lys Leu Ile Val Arg Glu Ala 
                85                  90                  95      


Glu Lys Asn Ala Asp Arg Ile Ile Asn Glu Ser Leu Ser Lys Ser Arg 
            100                 105                 110         


Lys Ile Ala Met Glu Ile Glu Glu Leu Lys Lys Gln Ser Lys Val Phe 
        115                 120                 125             


Arg Thr Arg Phe Gln Met Leu Ile Glu Ala Gln Leu Asp Leu Leu Lys 
    130                 135                 140                 


Asn Asp Asp Trp Asp His Leu Leu Glu Tyr Glu Val Asp Ala Val Phe 
145                 150                 155                 160 


Glu Glu Lys Glu 
                


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5
cccgcagctg agcttccgtt atcg                                              24


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6
cccgcagctg agcttccaat atcg                                              24


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  7
aacacgcgca ggctggttgt g                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8
aacacgcgca ggctgattgt g                                                 21


<210>  9

<400>  9
000

<210>  10
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10
ggatgagagg gatgaaaaac tcaaggcaga gataactctg attacacgtc ttgagcgat        59


<210>  11
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11
ataaatggga gggtgacttg cctcaatata atccagacta ctgacatggg aattagcca        59


<210>  12
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12
ctttgccata aaattctgct gggatttgat cactcagcat tacacgtctt gagcgat          57


<210>  13
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13
ctggcgatga ttaatagcta attgagtaag gccaggactg acatgggaat tagcca           56


<210>  14
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14
acaagcgttt gaggaagcct ttcttctctt cttcaatgaa attacacgtc ttgagcgat        59


<210>  15
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15
caaggaattt ctatggcacg cattattgtt gttacttcgg ctgacatggg aattagcca        59


<210>  16
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16
ttgagtaagg ccaggatgtc aaacacgcca atcgagcttt tgtgtaggct ggagctgct        59


<210>  17
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17
ctttttgctc aagctggacg gtaaccattt ccggatcgat cggctgacat gggaattagc       60


<210>  18
<211>  3882
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18
tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga       60

acttttgctg agttgaagga tcagcagtgc tcttgtgggt ccgattcgcc agatgataag      120

gaacagagac gggaccaaaa cgaaaaaagg cccccctttc gggaggcctc ttttctggaa      180

tttggtaccg aggcattcag tctgcctgct ttcgcaggaa cgccggaatg tccagataat      240

ccggctcttt ggctgtctga ggagcattat cattaacaac tttggcgact ggtttctgct      300

cctgcgtaag gggcgccata ccatgttgtt gatagcggtc catcacaggc tgctgaacct      360

gtttattagt cactaaggta atttctggtc ttttatccat gccgattccc gtggcgacaa      420

ccgttacgcg cagttcatca ttcatgtcag ggtctaagga ggtacctata actacggtcg      480

cgttgtctga ggcaaatgct ctaatggtgt ttcccacagt ctcgaactca tctaaccgaa      540

gatcgaaacc ggcagtaata tttacaagaa caccgcgggc gccggacaaa tcaatatcct      600

ctaataacgg tgaagatata gccatctcag cagcttcttc tgcgcgatcc tccccagatg      660

ccacgcctga gcccatcatg gcatagccca tctctgacat cactgtcctc acatccgcaa      720

aatctacgtt cataagccca ggtcgggtga tcaattcggc tataccctga accgcgcctt      780

tcagaacatc attagccgca ccgaacgcat ccagcagtga gattcctcgc cccaggactt      840

taagcagttt atcatttggg atcgtgatca atgaatcaac atgtttagaa agctccgtga      900

tgccctgctc cgcaaatgcc atacgtttct taccttcaaa gttgaacggt ttggtaacta      960

cggcaacggt cagaataccc agatctttgg ccacctccgc cacaaccggc gccgcgcccg     1020

tccccgtgcc gccgcccatg cctgcggcga taaaaaccat atctgcccct tccaatgcgg     1080

ctctcaaggc gtcgcgatcc tcatccgccg cgttgcgtcc tacttccgga ttcgccccag     1140

cacccaaacc tttggtgatg cctgacccga tctgaatagt ttgacctacg gcggttttac     1200

ggagtgcctg agcatctgtg ttcactgcaa agaattccac accctcaatg cgttctctaa     1260

ccatatgctc aacagcgttg ccgccaccac cgccgactcc tatgacttta ataaccgcat     1320

cgttggtaag ttccattggc tcaaacatct gatcctcctt agtatagtga cctcttaaac     1380

aaaattattt gtagaggcgc tttcgtcctc acggactcat cagaacggga agcacacccg     1440

ttgagcgctt tcacttttct ctatcactga tagggagtgg taaaataact ctatcaatga     1500

tagagtgtca acaaaaatta ggaattaatg atgtctagat tagataaaag taaagtgatt     1560

aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc     1620

gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct     1680

ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta     1740

gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta     1800

ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat     1860

gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca     1920

ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag     1980

catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta     2040

cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt     2100

gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc ttaaaatcag     2160

agaatataaa aagccagatt attaatccgg cttttttatt atttcgtctc ggtggaaggg     2220

ctcggagttg ccaggataca tagattacca cccgtagaaa agatcaaaag atcttcttga     2280

gatccttttt ttctgcgcgt aatctgctac ttgcaaacaa aaaaaccacc gctaccagcg     2340

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggctttagc     2400

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     2460

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     2520

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     2580

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     2640

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     2700

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     2760

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     2820

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     2880

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatttccgc tactgaacta     2940

ctcgatgtca gagccagcgt cttgcataaa aaaacccgcc gaagcgggtt tttacgttat     3000

tattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca     3060

ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc     3120

caaagaatgg caaggtcctg gtaacggtct gcgattccga cccgtccaac atcaatacaa     3180

cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg     3240

actgaatccg gtgagaatgg caagagcttg tgcatttctt tccagacttg ttcaacaggc     3300

cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt catgcgtgat     3360

tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag gacaattaca aacaggaatc     3420

gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa tattttcacc tgaatcagga     3480

tattcttcta atacctggaa ggctgttttc ccaggaatcg cggtggtgag taaccacgca     3540

tcatcaggag tacggataaa atgcttgatg gtcgggagag gcataaactc cgtcagccag     3600

ttgaggcgga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga     3660

aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg     3720

acattatcgc gagcccattt atacccatat aaatcagcgt ccatgttgga gtttaagcgc     3780

ggacgggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt     3840

atgtaagcag acagttttat tgttcatgat gatatatttt ta                        3882


