                         SEQUENCE LISTING

<110>  Fate Therapeutics, Inc.
 
<120>  COMBINING iPSC-DERIVED EFFECTOR CELL TYPES FOR IMMUNOTHERAPY USE

<130>  056932-527001WO

<150>  US 63/041,672
<151>  2020-06-19

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD64 domain-based construction 

<400>  1

Met Trp Phe Leu Thr Thr Leu Leu Leu Trp Val Pro Val Asp Gly Gln 
1               5                   10                  15      


Val Asp Thr Thr Lys Ala Val Ile Thr Leu Gln Pro Pro Trp Val Ser 
            20                  25                  30          


Val Phe Gln Glu Glu Thr Val Thr Leu His Cys Glu Val Leu His Leu 
        35                  40                  45              


Pro Gly Ser Ser Ser Thr Gln Trp Phe Leu Asn Gly Thr Ala Thr Gln 
    50                  55                  60                  


Thr Ser Thr Pro Ser Tyr Arg Ile Thr Ser Ala Ser Val Asn Asp Ser 
65                  70                  75                  80  


Gly Glu Tyr Arg Cys Gln Arg Gly Leu Ser Gly Arg Ser Asp Pro Ile 
                85                  90                  95      


Gln Leu Glu Ile His Arg Gly Trp Leu Leu Leu Gln Val Ser Ser Arg 
            100                 105                 110         


Val Phe Thr Glu Gly Glu Pro Leu Ala Leu Arg Cys His Ala Trp Lys 
        115                 120                 125             


Asp Lys Leu Val Tyr Asn Val Leu Tyr Tyr Arg Asn Gly Lys Ala Phe 
    130                 135                 140                 


Lys Phe Phe His Trp Asn Ser Asn Leu Thr Ile Leu Lys Thr Asn Ile 
145                 150                 155                 160 


Ser His Asn Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Arg Tyr 
                165                 170                 175     


Thr Ser Ala Gly Ile Ser Val Thr Val Lys Glu Leu Phe Pro Ala Pro 
            180                 185                 190         


Val Leu Asn Ala Ser Val Thr Ser Pro Leu Leu Glu Gly Asn Leu Val 
        195                 200                 205             


Thr Leu Ser Cys Glu Thr Lys Leu Leu Leu Gln Arg Pro Gly Leu Gln 
    210                 215                 220                 


Leu Tyr Phe Ser Phe Tyr Met Gly Ser Lys Thr Leu Arg Gly Arg Asn 
225                 230                 235                 240 


Thr Ser Ser Glu Tyr Gln Ile Leu Thr Ala Arg Arg Glu Asp Ser Gly 
                245                 250                 255     


Leu Tyr Trp Cys Glu Ala Ala Thr Glu Asp Gly Asn Val Leu Lys Arg 
            260                 265                 270         


Ser Pro Glu Leu Glu Leu Gln Val Leu Gly Leu Gln Leu Pro Thr Pro 
        275                 280                 285             


Val Trp Phe His Tyr Gln Val Ser Phe Cys Leu Val Met Val Leu Leu 
    290                 295                 300                 


Phe Ala Val Asp Thr Gly Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg 
305                 310                 315                 320 


Ser Ser Thr Arg Asp Trp Lys Asp His Lys Phe Lys Trp Arg Lys Asp 
                325                 330                 335     


Pro Gln Asp Lys 
            340 


<210>  2
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD64 exon-based construction

<400>  2

Met Trp Phe Leu Thr Thr Leu Leu Leu Trp Val Pro Val Asp Gly Gln 
1               5                   10                  15      


Val Asp Thr Thr Lys Ala Val Ile Thr Leu Gln Pro Pro Trp Val Ser 
            20                  25                  30          


Val Phe Gln Glu Glu Thr Val Thr Leu His Cys Glu Val Leu His Leu 
        35                  40                  45              


Pro Gly Ser Ser Ser Thr Gln Trp Phe Leu Asn Gly Thr Ala Thr Gln 
    50                  55                  60                  


Thr Ser Thr Pro Ser Tyr Arg Ile Thr Ser Ala Ser Val Asn Asp Ser 
65                  70                  75                  80  


Gly Glu Tyr Arg Cys Gln Arg Gly Leu Ser Gly Arg Ser Asp Pro Ile 
                85                  90                  95      


Gln Leu Glu Ile His Arg Gly Trp Leu Leu Leu Gln Val Ser Ser Arg 
            100                 105                 110         


Val Phe Thr Glu Gly Glu Pro Leu Ala Leu Arg Cys His Ala Trp Lys 
        115                 120                 125             


Asp Lys Leu Val Tyr Asn Val Leu Tyr Tyr Arg Asn Gly Lys Ala Phe 
    130                 135                 140                 


Lys Phe Phe His Trp Asn Ser Asn Leu Thr Ile Leu Lys Thr Asn Ile 
145                 150                 155                 160 


Ser His Asn Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Arg Tyr 
                165                 170                 175     


Thr Ser Ala Gly Ile Ser Val Thr Val Lys Glu Leu Phe Pro Ala Pro 
            180                 185                 190         


Val Leu Asn Ala Ser Val Thr Ser Pro Leu Leu Glu Gly Asn Leu Val 
        195                 200                 205             


Thr Leu Ser Cys Glu Thr Lys Leu Leu Leu Gln Arg Pro Gly Leu Gln 
    210                 215                 220                 


Leu Tyr Phe Ser Phe Tyr Met Gly Ser Lys Thr Leu Arg Gly Arg Asn 
225                 230                 235                 240 


Thr Ser Ser Glu Tyr Gln Ile Leu Thr Ala Arg Arg Glu Asp Ser Gly 
                245                 250                 255     


Leu Tyr Trp Cys Glu Ala Ala Thr Glu Asp Gly Asn Val Leu Lys Arg 
            260                 265                 270         


Ser Pro Glu Leu Glu Leu Gln Val Leu Gly Leu Phe Phe Pro Pro Gly 
        275                 280                 285             


Tyr Gln Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp 
    290                 295                 300                 


Thr Gly Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg 
305                 310                 315                 320 


Asp Trp Lys Asp His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
                325                 330                 335     


<210>  3
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD64 exon-based construction

<400>  3

Met Trp Phe Leu Thr Thr Leu Leu Leu Trp Val Pro Val Asp Gly Gln 
1               5                   10                  15      


Val Asp Thr Thr Lys Ala Val Ile Thr Leu Gln Pro Pro Trp Val Ser 
            20                  25                  30          


Val Phe Gln Glu Glu Thr Val Thr Leu His Cys Glu Val Leu His Leu 
        35                  40                  45              


Pro Gly Ser Ser Ser Thr Gln Trp Phe Leu Asn Gly Thr Ala Thr Gln 
    50                  55                  60                  


Thr Ser Thr Pro Ser Tyr Arg Ile Thr Ser Ala Ser Val Asn Asp Ser 
65                  70                  75                  80  


Gly Glu Tyr Arg Cys Gln Arg Gly Leu Ser Gly Arg Ser Asp Pro Ile 
                85                  90                  95      


Gln Leu Glu Ile His Arg Gly Trp Leu Leu Leu Gln Val Ser Ser Arg 
            100                 105                 110         


Val Phe Thr Glu Gly Glu Pro Leu Ala Leu Arg Cys His Ala Trp Lys 
        115                 120                 125             


Asp Lys Leu Val Tyr Asn Val Leu Tyr Tyr Arg Asn Gly Lys Ala Phe 
    130                 135                 140                 


Lys Phe Phe His Trp Asn Ser Asn Leu Thr Ile Leu Lys Thr Asn Ile 
145                 150                 155                 160 


Ser His Asn Gly Thr Tyr His Cys Ser Gly Met Gly Lys His Arg Tyr 
                165                 170                 175     


Thr Ser Ala Gly Ile Ser Val Thr Val Lys Glu Leu Phe Pro Ala Pro 
            180                 185                 190         


Val Leu Asn Ala Ser Val Thr Ser Pro Leu Leu Glu Gly Asn Leu Val 
        195                 200                 205             


Thr Leu Ser Cys Glu Thr Lys Leu Leu Leu Gln Arg Pro Gly Leu Gln 
    210                 215                 220                 


Leu Tyr Phe Ser Phe Tyr Met Gly Ser Lys Thr Leu Arg Gly Arg Asn 
225                 230                 235                 240 


Thr Ser Ser Glu Tyr Gln Ile Leu Thr Ala Arg Arg Glu Asp Ser Gly 
                245                 250                 255     


Leu Tyr Trp Cys Glu Ala Ala Thr Glu Asp Gly Asn Val Leu Lys Arg 
            260                 265                 270         


Ser Pro Glu Leu Glu Leu Gln Val Leu Gly Phe Phe Pro Pro Gly Tyr 
        275                 280                 285             


Gln Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr 
    290                 295                 300                 


Gly Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp 
305                 310                 315                 320 


Trp Lys Asp His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
                325                 330                 335 


<210>  4
<211>  1032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD64 domain-based construction (encoding SEQ ID NO. 1)

<400>  4
cttggagaca acatgtggtt cttgacaact ctgctccttt gggttccagt tgatgggcaa       60

gtggacacca caaaggcagt gatcactttg cagcctccat gggtcagcgt gttccaagag      120

gaaaccgtaa ccttgcattg tgaggtgctc catctgcctg ggagcagctc tacacagtgg      180

tttctcaatg gcacagccac tcagacctcg acccccagct acagaatcac ctctgccagt      240

gtcaatgaca gtggtgaata caggtgccag agaggtctct cagggcgaag tgaccccata      300

cagctggaaa tccacagagg ctggctacta ctgcaggtct ccagcagagt cttcacggaa      360

ggagaacctc tggccttgag gtgtcatgcg tggaaggata agctggtgta caatgtgctt      420

tactatcgaa atggcaaagc ctttaagttt ttccactgga attctaacct caccattctg      480

aaaaccaaca taagtcacaa tggcacctac cattgctcag gcatgggaaa gcatcgctac      540

acatcagcag gaatatctgt cactgtgaaa gagctatttc cagctccagt gctgaatgca      600

tctgtgacat ccccactcct ggaggggaat ctggtcaccc tgagctgtga aacaaagttg      660

ctcttgcaga ggcctggttt gcagctttac ttctccttct acatgggcag caagaccctg      720

cgaggcagga acacatcctc tgaataccaa atactaactg ctagaagaga agactctggg      780

ttatactggt gcgaggctgc cacagaggat ggaaatgtcc ttaagcgcag ccctgagttg      840

gagcttcaag tgcttggcct ccagttacca actcctgtct ggtttcatta ccaagtctct      900

ttctgcttgg tgatggtact cctttttgca gtggacacag gactatattt ctctgtgaag      960

acaaacattc gaagctcaac aagagactgg aaggaccata aatttaaatg gagaaaggac     1020

cctcaagaca aa                                                         1032


<210>  5
<211>  1020
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD64 exon-based construction (encoding SEQ ID NO. 2)

<400>  5
cttggagaca acatgtggtt cttgacaact ctgctccttt gggttccagt tgatgggcaa       60

gtggacacca caaaggcagt gatcactttg cagcctccat gggtcagcgt gttccaagag      120

gaaaccgtaa ccttgcattg tgaggtgctc catctgcctg ggagcagctc tacacagtgg      180

tttctcaatg gcacagccac tcagacctcg acccccagct acagaatcac ctctgccagt      240

gtcaatgaca gtggtgaata caggtgccag agaggtctct cagggcgaag tgaccccata      300

cagctggaaa tccacagagg ctggctacta ctgcaggtct ccagcagagt cttcacggaa      360

ggagaacctc tggccttgag gtgtcatgcg tggaaggata agctggtgta caatgtgctt      420

tactatcgaa atggcaaagc ctttaagttt ttccactgga attctaacct caccattctg      480

aaaaccaaca taagtcacaa tggcacctac cattgctcag gcatgggaaa gcatcgctac      540

acatcagcag gaatatctgt cactgtgaaa gagctatttc cagctccagt gctgaatgca      600

tctgtgacat ccccactcct ggaggggaat ctggtcaccc tgagctgtga aacaaagttg      660

ctcttgcaga ggcctggttt gcagctttac ttctccttct acatgggcag caagaccctg      720

cgaggcagga acacatcctc tgaataccaa atactaactg ctagaagaga agactctggg      780

ttatactggt gcgaggctgc cacagaggat ggaaatgtcc ttaagcgcag ccctgagttg      840

gagcttcaag tgcttggttt gttctttcca cctgggtacc aagtctcttt ctgcttggtg      900

atggtactcc tttttgcagt ggacacagga ctatatttct ctgtgaagac aaacattcga      960

agctcaacaa gagactggaa ggaccataaa tttaaatgga gaaaggaccc tcaagacaaa     1020


<210>  6
<211>  1005
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD64 exon-based construction (encoding SEQ ID NO. 3)

<400>  6
atgtggttct tgacaactct gctcctttgg gttccagttg atgggcaagt ggacaccaca       60

aaggcagtga tcactttgca gcctccatgg gtcagcgtgt tccaagagga aaccgtaacc      120

ttgcactgtg aggtgctcca tctgcctggg agcagctcta cacagtggtt tctcaatggc      180

acagccactc agacctcgac ccccagctac agaatcacct ctgccagtgt caatgacagt      240

ggtgaataca ggtgccagag aggtctctca gggcgaagtg accccataca gctggaaatc      300

cacagaggct ggctactact gcaggtctcc agcagagtct tcacggaagg agaacctctg      360

gccttgaggt gtcatgcgtg gaaggataag ctggtgtaca atgtgcttta ctatcgaaat      420

ggcaaagcct ttaagttttt ccactggaac tctaacctca ccattctgaa aaccaacata      480

agtcacaatg gcacctacca ttgctcaggc atgggaaagc atcgctacac atcagcagga      540

atatctgtca ctgtgaaaga gctatttcca gctccagtgc tgaatgcatc tgtgacatcc      600

ccactcctgg aggggaatct ggtcaccctg agctgtgaaa caaagttgct cttgcagagg      660

cctggtttgc agctttactt ctccttctac atgggcagca agaccctgcg aggcaggaac      720

acatcctctg aataccaaat actaactgct agaagagaag actctgggtt atactggtgc      780

gaggctgcca cagaggatgg aaatgtcctt aagcgcagcc ctgagttgga gcttcaagtg      840

cttggcttct ttccacctgg gtaccaagtc tctttctgct tggtgatggt actccttttt      900

gcagtggaca caggactata tttctctgtg aagacaaaca ttcgaagctc aacaagagac      960

tggaaggacc ataaatttaa atggagaaag gaccctcaag acaaa                     1005


<210>  7
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 co-stim + CD3-zeta-ITAM construct

<400>  7

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      


Glu Gly Leu Phe Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Phe 
            100                 105                 110         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             


Gly Leu Phe Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Phe Asp Ala 
    130                 135                 140                 


Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             


<210>  8
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 hinge + CD28 TM + CD28 co-stim + CD3-zeta-ITAM construct

<400>  8

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 
        35                  40                  45              


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
    50                  55                  60                  


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
65                  70                  75                  80  


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
                85                  90                  95      


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser 
            100                 105                 110         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        115                 120                 125             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    130                 135                 140                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
145                 150                 155                 160 


Pro Gln Glu Gly Leu Phe Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                165                 170                 175     


Ala Phe Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            180                 185                 190         


His Asp Gly Leu Phe Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Phe 
        195                 200                 205             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    210                 215                 


<210>  9
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NKG2D TM + 2B4 + CD3-zeta construct

<400>  9

Ser Asn Leu Phe Val Ala Ser Trp Ile Ala Val Met Ile Ile Phe Arg 
1               5                   10                  15      


Ile Gly Met Ala Val Ala Ile Phe Cys Cys Phe Phe Phe Pro Ser Trp 
            20                  25                  30          


Arg Arg Lys Arg Lys Glu Lys Gln Ser Glu Thr Ser Pro Lys Glu Phe 
        35                  40                  45              


Leu Thr Ile Tyr Glu Asp Val Lys Asp Leu Lys Thr Arg Arg Asn His 
    50                  55                  60                  


Glu Gln Glu Gln Thr Phe Pro Gly Gly Gly Ser Thr Ile Tyr Ser Met 
65                  70                  75                  80  


Ile Gln Ser Gln Ser Ser Ala Pro Thr Ser Gln Glu Pro Ala Tyr Thr 
                85                  90                  95      


Leu Tyr Ser Leu Ile Gln Pro Ser Arg Lys Ser Gly Ser Arg Lys Arg 
            100                 105                 110         


Asn His Ser Pro Ser Phe Asn Ser Thr Ile Tyr Glu Val Ile Gly Lys 
        115                 120                 125             


Ser Gln Pro Lys Ala Gln Asn Pro Ala Arg Leu Ser Arg Lys Glu Leu 
    130                 135                 140                 


Glu Asn Phe Asp Val Tyr Ser Arg Val Lys Phe Ser Arg Ser Ala Asp 
145                 150                 155                 160 


Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
                165                 170                 175     


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
            180                 185                 190         


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
        195                 200                 205             


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
    210                 215                 220                 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
225                 230                 235                 240 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
                245                 250                 255     


Met Gln Ala Leu Pro Pro Arg 
            260             


<210>  10
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge + NKG2D TM + 2B4 + CD3-zeta construct

<400>  10

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ser Asn Leu 
        35                  40                  45              


Phe Val Ala Ser Trp Ile Ala Val Met Ile Ile Phe Arg Ile Gly Met 
    50                  55                  60                  


Ala Val Ala Ile Phe Cys Cys Phe Phe Phe Pro Ser Trp Arg Arg Lys 
65                  70                  75                  80  


Arg Lys Glu Lys Gln Ser Glu Thr Ser Pro Lys Glu Phe Leu Thr Ile 
                85                  90                  95      


Tyr Glu Asp Val Lys Asp Leu Lys Thr Arg Arg Asn His Glu Gln Glu 
            100                 105                 110         


Gln Thr Phe Pro Gly Gly Gly Ser Thr Ile Tyr Ser Met Ile Gln Ser 
        115                 120                 125             


Gln Ser Ser Ala Pro Thr Ser Gln Glu Pro Ala Tyr Thr Leu Tyr Ser 
    130                 135                 140                 


Leu Ile Gln Pro Ser Arg Lys Ser Gly Ser Arg Lys Arg Asn His Ser 
145                 150                 155                 160 


Pro Ser Phe Asn Ser Thr Ile Tyr Glu Val Ile Gly Lys Ser Gln Pro 
                165                 170                 175     


Lys Ala Gln Asn Pro Ala Arg Leu Ser Arg Lys Glu Leu Glu Asn Phe 
            180                 185                 190         


Asp Val Tyr Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
        195                 200                 205             


Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
    210                 215                 220                 


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
225                 230                 235                 240 


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
                245                 250                 255     


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            260                 265                 270         


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
        275                 280                 285             


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
    290                 295                 300                 


Leu Pro Pro Arg 
305             


<210>  11
<211>  414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a construct mimicking trans-presentation of IL15 (Design 2)

<400>  11

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu 
            20                  25                  30          


Pro Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
        35                  40                  45              


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
    50                  55                  60                  


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
65                  70                  75                  80  


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
                85                  90                  95      


His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser 
            100                 105                 110         


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
        115                 120                 125             


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
    130                 135                 140                 


Gln Met Phe Ile Asn Thr Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Leu 
                165                 170                 175     


Gln Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp 
            180                 185                 190         


Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser 
        195                 200                 205             


Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu 
    210                 215                 220                 


Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys 
225                 230                 235                 240 


Ile Arg Asp Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr 
                245                 250                 255     


Val Thr Thr Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser 
            260                 265                 270         


Gly Lys Glu Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala 
        275                 280                 285             


Thr Thr Ala Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser 
    290                 295                 300                 


Pro Ser Thr Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly 
305                 310                 315                 320 


Thr Pro Ser Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala 
                325                 330                 335     


Ser His Gln Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr 
            340                 345                 350         


Val Ala Ile Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val 
        355                 360                 365             


Ser Leu Leu Ala Cys Tyr Leu Lys Ser Arg Gln Thr Pro Pro Leu Ala 
    370                 375                 380                 


Ser Val Glu Met Glu Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr 
385                 390                 395                 400 


Ser Ser Arg Asp Glu Asp Leu Glu Asn Cys Ser His His Leu 
                405                 410                 


<210>  12
<211>  1242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary DNA sequence encoding the amino acid sequence of 
SEQ ID NO: 11

<400>  12
atggactgga cctggattct gttcctggtc gcggctgcaa cgcgagtcca tagcggtatc       60

catgttttta ttcttgggtg tttttctgct gggctgccta agaccgaggc caactgggta      120

aatgtcatca gtgacctcaa gaaaatagaa gaccttatac aaagcatgca cattgatgct      180

actctctaca ctgagtcaga tgtacatccc tcatgcaaag tgacggccat gaaatgtttc      240

ctcctcgaac ttcaagtcat atctctggaa agtggcgacg cgtccatcca cgacacggtc      300

gaaaacctga taatactcgc taataatagt ctctcttcaa atggtaacgt aaccgagtca      360

ggttgcaaag agtgcgaaga gttggaagaa aaaaacataa aggagttcct gcaaagtttc      420

gtgcacattg tgcagatgtt cattaatacc tctagcggcg gaggatcagg tggcggtgga      480

agcggaggtg gaggctccgg tggaggaggt agtggcggag gttctcttca aataacttgt      540

cctccaccga tgtccgtaga acatgcggat atttgggtaa aatcctatag cttgtacagc      600

cgagagcggt atatctgcaa cagcggcttc aagcggaagg ccggcacaag cagcctgacc      660

gagtgcgtgc tgaacaaggc caccaacgtg gcccactgga ccacccctag cctgaagtgc      720

atcagagatc ccgccctggt gcatcagcgg cctgcccctc caagcacagt gacaacagct      780

ggcgtgaccc cccagcctga gagcctgagc ccttctggaa aagagcctgc cgccagcagc      840

cccagcagca acaatactgc cgccaccaca gccgccatcg tgcctggatc tcagctgatg      900

cccagcaaga gccctagcac cggcaccacc gagatcagca gccacgagtc tagccacggc      960

accccatctc agaccaccgc caagaactgg gagctgacag ccagcgcctc tcaccagcct     1020

ccaggcgtgt accctcaggg ccacagcgat accacagtgg ccatcagcac ctccaccgtg     1080

ctgctgtgtg gactgagcgc cgtgtcactg ctggcctgct acctgaagtc cagacagacc     1140

cctccactgg ccagcgtgga aatggaagcc atggaagcac tgcccgtgac ctggggcacc     1200

agctccagag atgaggatct ggaaaactgc tcccaccacc tg                        1242


<210>  13
<211>  379
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a construct mimicking trans-presentation of IL15 (Design 3)

<400>  13

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu 
            20                  25                  30          


Pro Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
        35                  40                  45              


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
    50                  55                  60                  


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
65                  70                  75                  80  


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
                85                  90                  95      


His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser 
            100                 105                 110         


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
        115                 120                 125             


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
    130                 135                 140                 


Gln Met Phe Ile Asn Thr Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Leu 
                165                 170                 175     


Gln Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp 
            180                 185                 190         


Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser 
        195                 200                 205             


Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu 
    210                 215                 220                 


Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys 
225                 230                 235                 240 


Ile Arg Asp Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr 
                245                 250                 255     


Val Thr Thr Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser 
            260                 265                 270         


Gly Lys Glu Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala 
        275                 280                 285             


Thr Thr Ala Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser 
    290                 295                 300                 


Pro Ser Thr Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly 
305                 310                 315                 320 


Thr Pro Ser Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala 
                325                 330                 335     


Ser His Gln Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr 
            340                 345                 350         


Val Ala Ile Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val 
        355                 360                 365             


Ser Leu Leu Ala Cys Tyr Leu Lys Ser Arg Gln 
    370                 375                 


<210>  14
<211>  1140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary nucleic acid sequence encoding the amino acid sequence of 
SEQ ID NO: 13

<400>  14
atggactgga cctggattct gttcctggtc gcggctgcaa cgcgagtcca tagcggtatc       60

catgttttta ttcttgggtg tttttctgct gggctgccta agaccgaggc caactgggta      120

aatgtcatca gtgacctcaa gaaaatagaa gaccttatac aaagcatgca cattgatgct      180

actctctaca ctgagtcaga tgtacatccc tcatgcaaag tgacggccat gaaatgtttc      240

ctcctcgaac ttcaagtcat atctctggaa agtggcgacg cgtccatcca cgacacggtc      300

gaaaacctga taatactcgc taataatagt ctctcttcaa atggtaacgt aaccgagtca      360

ggttgcaaag agtgcgaaga gttggaagaa aaaaacataa aggagttcct gcaaagtttc      420

gtgcacattg tgcagatgtt cattaatacc tctagcggcg gaggatcagg tggcggtgga      480

agcggaggtg gaggctccgg tggaggaggt agtggcggag gttctcttca aataacttgt      540

cctccaccga tgtccgtaga acatgcggat atttgggtaa aatcctatag cttgtacagc      600

cgagagcggt atatctgcaa cagcggcttc aagcggaagg ccggcacaag cagcctgacc      660

gagtgcgtgc tgaacaaggc caccaacgtg gcccactgga ccacccctag cctgaagtgc      720

atcagagatc ccgccctggt gcatcagcgg cctgcccctc caagcacagt gacaacagct      780

ggcgtgaccc cccagcctga gagcctgagc ccttctggaa aagagcctgc cgccagcagc      840

cccagcagca acaatactgc cgccaccaca gccgccatcg tgcctggatc tcagctgatg      900

cccagcaaga gccctagcac cggcaccacc gagatcagca gccacgagtc tagccacggc      960

accccatctc agaccaccgc caagaactgg gagctgacag ccagcgcctc tcaccagcct     1020

ccaggcgtgt accctcaggg ccacagcgat accacagtgg ccatcagcac ctccaccgtg     1080

ctgctgtgtg gactgagcgc cgtgtcactg ctggcctgct acctgaagtc cagacagtga     1140


<210>  15
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  a construct mimicking trans-presentation of IL15 (Design 4)

<400>  15

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu 
            20                  25                  30          


Pro Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys 
        35                  40                  45              


Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr 
    50                  55                  60                  


Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe 
65                  70                  75                  80  


Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile 
                85                  90                  95      


His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser 
            100                 105                 110         


Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu 
        115                 120                 125             


Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val 
    130                 135                 140                 


Gln Met Phe Ile Asn Thr Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Leu 
                165                 170                 175     


Gln Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp 
            180                 185                 190         


Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser 
        195                 200                 205             


Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu 
    210                 215                 220                 


Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys 
225                 230                 235                 240 


Ile Arg 
        


<210>  16
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary nucleic acid sequence encoding the amino acid sequence of 
SEQ ID NO: 15

<400>  16
atggactgga cctggattct gttcctggtc gcggctgcaa cgcgagtcca tagcggtatc       60

catgttttta ttcttgggtg tttttctgct gggctgccta agaccgaggc caactgggta      120

aatgtcatca gtgacctcaa gaaaatagaa gaccttatac aaagcatgca cattgatgct      180

actctctaca ctgagtcaga tgtacatccc tcatgcaaag tgacggccat gaaatgtttc      240

ctcctcgaac ttcaagtcat atctctggaa agtggcgacg cgtccatcca cgacacggtc      300

gaaaacctga taatactcgc taataatagt ctctcttcaa atggtaacgt aaccgagtca      360

ggttgcaaag agtgcgaaga gttggaagaa aaaaacataa aggagttcct gcaaagtttc      420

gtgcacattg tgcagatgtt cattaatacc tctagcggcg gaggatcagg tggcggtgga      480

agcggaggtg gaggctccgg tggaggaggt agtggcggag gttctcttca aataacttgt      540

cctccaccga tgtccgtaga acatgcggat atttgggtaa aatcctatag cttgtacagc      600

cgagagcggt atatctgcaa cagcggcttc aagcggaagg ccggcacaag cagcctgacc      660

gagtgcgtgc tgaacaaggc caccaacgtg gcccactgga ccacccctag cctgaagtgc      720

atcaga                                                                 726


