                         SEQUENCE LISTING

<110>  Nanyang Technological University
 
<120>  CRISPR-BASED METHODS FOR THE DETECTION OF NUCLEIC ACIDS IN A 
       SAMPLE

<130>  P120277

<150>  SG10202006167W
<151>  2020-06-26

<160>  290   

<170>  PatentIn version 3.5

<210>  1
<211>  1307
<212>  PRT
<213>  Artificial

<220>
<223>  engineered AsCas12a

<400>  1

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Arg Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp 
    530                 535                 540                 


Asp Val Asn Arg Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  2
<211>  1307
<212>  PRT
<213>  Acidaminococcus sp. BV3L6

<400>  2

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  3
<211>  1228
<212>  PRT
<213>  Lachnospiraceae bacterium ND2006

<400>  3

Ala Ala Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys 
1               5                   10                  15      


Thr Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile 
            20                  25                  30          


Asp Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr 
        35                  40                  45              


Lys Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn 
    50                  55                  60                  


Asp Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser 
65                  70                  75                  80  


Leu Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu 
                85                  90                  95      


Asn Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly 
            100                 105                 110         


Ala Ala Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile 
        115                 120                 125             


Leu Pro Glu Ala Ala Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser 
    130                 135                 140                 


Phe Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu 
145                 150                 155                 160 


Asn Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys 
                165                 170                 175     


Ile Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu 
            180                 185                 190         


Lys Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu 
        195                 200                 205             


Lys Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu 
    210                 215                 220                 


Phe Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala 
225                 230                 235                 240 


Ile Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu 
                245                 250                 255     


Asn Glu Tyr Ile Asn Leu Tyr Asn Ala Lys Thr Lys Gln Ala Leu Pro 
            260                 265                 270         


Lys Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu 
        275                 280                 285             


Ser Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val 
    290                 295                 300                 


Phe Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys 
305                 310                 315                 320 


Lys Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly 
                325                 330                 335     


Ile Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile 
            340                 345                 350         


Phe Gly Glu Trp Asn Leu Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp 
        355                 360                 365             


Asp Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp 
    370                 375                 380                 


Asp Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln 
385                 390                 395                 400 


Leu Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys 
                405                 410                 415     


Glu Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser 
            420                 425                 430         


Ser Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys 
        435                 440                 445             


Lys Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val 
    450                 455                 460                 


Lys Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu 
465                 470                 475                 480 


Thr Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp 
                485                 490                 495     


Ile Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val 
            500                 505                 510         


Thr Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn 
        515                 520                 525             


Pro Gln Phe Met Gly Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg 
    530                 535                 540                 


Ala Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp 
545                 550                 555                 560 


Lys Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn 
                565                 570                 575     


Gly Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys 
            580                 585                 590         


Met Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn 
        595                 600                 605             


Pro Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys 
    610                 615                 620                 


Gly Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe 
625                 630                 635                 640 


Lys Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe 
                645                 650                 655     


Asn Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg 
            660                 665                 670         


Glu Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys 
        675                 680                 685             


Lys Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln 
    690                 695                 700                 


Ile Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu 
705                 710                 715                 720 


His Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln 
                725                 730                 735     


Ile Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu 
            740                 745                 750         


Lys Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn 
        755                 760                 765             


Lys Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val 
    770                 775                 780                 


Tyr Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro 
785                 790                 795                 800 


Ile Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu 
                805                 810                 815     


Val Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile 
            820                 825                 830         


Asp Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys 
        835                 840                 845             


Gly Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe 
    850                 855                 860                 


Asn Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys 
865                 870                 875                 880 


Glu Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn 
                885                 890                 895     


Ile Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile 
            900                 905                 910         


Cys Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu 
        915                 920                 925             


Asn Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr 
    930                 935                 940                 


Gln Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp 
945                 950                 955                 960 


Lys Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln 
                965                 970                 975     


Ile Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly 
            980                 985                 990         


Phe Ile Phe Tyr Ile Pro Ala Trp  Leu Thr Ser Lys Ile  Asp Pro Ser 
        995                 1000                 1005             


Thr Gly  Phe Val Asn Leu Leu  Lys Thr Lys Tyr Thr  Ser Ile Ala 
    1010                 1015                 1020             


Asp Ser  Lys Lys Phe Ile Ser  Ser Phe Asp Arg Ile  Met Tyr Val 
    1025                 1030                 1035             


Pro Glu  Glu Asp Leu Phe Glu  Phe Ala Leu Asp Tyr  Lys Asn Phe 
    1040                 1045                 1050             


Ser Arg  Thr Asp Ala Asp Tyr  Ile Lys Lys Trp Lys  Leu Tyr Ser 
    1055                 1060                 1065             


Tyr Gly  Asn Arg Ile Arg Ile  Phe Ala Ala Ala Lys  Lys Asn Asn 
    1070                 1075                 1080             


Val Phe  Ala Trp Glu Glu Val  Cys Leu Thr Ser Ala  Tyr Lys Glu 
    1085                 1090                 1095             


Leu Phe  Asn Lys Tyr Gly Ile  Asn Tyr Gln Gln Gly  Asp Ile Arg 
    1100                 1105                 1110             


Ala Leu  Leu Cys Glu Gln Ser  Asp Lys Ala Phe Tyr  Ser Ser Phe 
    1115                 1120                 1125             


Met Ala  Leu Met Ser Leu Met  Leu Gln Met Arg Asn  Ser Ile Thr 
    1130                 1135                 1140             


Gly Arg  Thr Asp Val Asp Phe  Leu Ile Ser Pro Val  Lys Asn Ser 
    1145                 1150                 1155             


Asp Gly  Ile Phe Tyr Asp Ser  Arg Asn Tyr Glu Ala  Gln Glu Asn 
    1160                 1165                 1170             


Ala Ile  Leu Pro Lys Asn Ala  Asp Ala Asn Gly Ala  Tyr Asn Ile 
    1175                 1180                 1185             


Ala Arg  Lys Val Leu Trp Ala  Ile Gly Gln Phe Lys  Lys Ala Glu 
    1190                 1195                 1200             


Asp Glu  Lys Leu Asp Lys Val  Lys Ile Ala Ile Ser  Asn Lys Glu 
    1205                 1210                 1215             


Trp Leu  Glu Tyr Ala Gln Thr  Ser Val Lys 
    1220                 1225             


<210>  4
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  FIP LAMP Primer

<400>  4
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg ct                          42


<210>  5
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  BIP LAMP Primer

<400>  5
tggacagctg gtgctaatag aaaagtccta ggttgaag                               38


<210>  6
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  F3 LAMP Primer

<400>  6
ttaatttagt gcgtgatctc c                                                 21


<210>  7
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  B3 LAMP Primer

<400>  7
agcatctgta atggttccat                                                   20


<210>  8
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  LF LAMP Primer

<400>  8
ggcaaatcta ccaatggttc taa                                               23


<210>  9
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  LB LAMP Primer

<400>  9
gcagcttatt atgtgggtta t                                                 21


<210>  10
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  F1c LAMP swarm Primer

<400>  10
tgtaaagcaa gtaaagtttg aaacc                                             25


<210>  11
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  B1c LAMP swarm Primer

<400>  11
tggacagctg gtgct                                                        15


<210>  12
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA spacer sequence

<400>  12
acuccuggug auucuucuuc                                                   20


<210>  13
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA spacer sequence

<400>  13
aaaccuagug auguuaauac                                                   20


<210>  14
<211>  20
<212>  RNA
<213>  SARS-CoV-2

<400>  14
gaagaagaau caccaggagu                                                   20


<210>  15
<211>  20
<212>  RNA
<213>  SARS-CoV-2

<400>  15
guauuaacau cacuagguuu                                                   20


<210>  16
<211>  20
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA Cas12a binding sequence

<400>  16
uaauuucuac ucuuguagau                                                   20


<210>  17
<211>  500
<212>  DNA
<213>  SARS-CoV-2

<400>  17
gctttgggct aagcgcaaca ttaaaccagt accagaggtg aaaatactca ataatttggg       60

tgtggacatt gctgctaata ctgtgatctg ggactacaaa agagatgctc cagcacatat      120

atctactatt ggtgtttgtt ctatgactga catagccaag aaaccaactg aaacgatttg      180

tgcaccactc actgtctttt ttgatggtag agttgatggt caagtagact tatttagaaa      240

tgcccgtaat ggtgttctta ttacagaagg tagtgttaaa ggtttacaac catctgtagg      300

tcccaaacaa gctagtctta atggagtcac attaattgga gaagccgtaa aaacacagtt      360

caattattat aagaaagttg atggtgttgt ccaacaatta cctgaaactt actttactca      420

gagtagaaat ttacaagaat ttaaacccag gagtcaaatg gaaattgatt tcttagaatt      480

agctatggat gaattcattg                                                  500


<210>  18
<211>  490
<212>  DNA
<213>  SARS-CoV-2

<400>  18
aaacagggta atttcaaaaa tcttagggaa tttgtgttta agaatattga tggttatttt       60

aaaatatatt ctaagcacac gcctattaat ttagtgcgtg atctccctca gggtttttcg      120

gctttagaac cattggtaga tttgccaata ggtattaaca tcactaggtt tcaaacttta      180

cttgctttac atagaagtta tttgactcct ggtgattctt cttcaggttg gacagctggt      240

gctgcagctt attatgtggg ttatcttcaa cctaggactt ttctattaaa atataatgaa      300

aatggaacca ttacagatgc tgtagactgt gcacttgacc ctctctcaga aacaaagtgt      360

acgttgaaat ccttcactgt agaaaaagga atctatcaaa cttctaactt tagagtccaa      420

ccaacagaat ctattgttag atttcctaat attacaaact tgtgcccttt tggtgaagtt      480

tttaacgcca                                                             490


<210>  19
<211>  490
<212>  DNA
<213>  SARS-CoV-2

<400>  19
cttctaagaa gcctcggcaa aaacgtactg ccactaaagc atacaatgta acacaagctt       60

tcggcagacg tggtccagaa caaacccaag gaaattttgg ggaccaggaa ctaatcagac      120

aaggaactga ttacaaacat tggccgcaaa ttgcacaatt tgcccccagc gcttcagcgt      180

tcttcggaat gtcgcgcatt ggcatggaag tcacaccttc gggaacgtgg ttgacctaca      240

caggtgccat caaattggat gacaaagatc caaatttcaa agatcaagtc attttgctga      300

ataagcatat tgacgcatac aaaacattcc caccaacaga gcctaaaaag gacaaaaaga      360

agaaggctga tgaaactcaa gccttaccgc agagacagaa gaaacagcaa actgtgactc      420

ttcttcctgc tgcagatttg gatgatttct ccaaacaatt gcaacaatcc atgagcagtg      480

ctgactcaac                                                             490


<210>  20
<211>  500
<212>  DNA
<213>  SARS-CoV

<400>  20
gctttgggct aagcgtaaca ttaaaccagt gccagagatt aagatactca ataatttggg       60

tgttgatatc gctgctaata ctgtaatctg ggactacaaa agagaagccc cagcacatgt      120

atctacaata ggtgtctgca caatgactga cattgccaag aaacctactg agagtgcttg      180

ttcttcactt actgtcttgt ttgatggtag agtggaagga caggtagacc tttttagaaa      240

cgcccgtaat ggtgttttaa taacagaagg ttcagtcaaa ggtctaacac cttcaaaggg      300

accagcacaa gctagcgtca atggagtcac attaattgga gaatcagtaa aaacacagtt      360

taactacttt aagaaagtag acggcattat tcaacagttg cctgaaacct actttactca      420

gagcagagac ttagaggatt ttaagcccag atcacaaatg gaaactgact ttctcgagct      480

cgctatggat gaattcatac                                                  500


<210>  21
<211>  482
<212>  DNA
<213>  SARS-CoV

<400>  21
aagtcaggta attttaaaca cttacgagag tttgtgttta aaaataaaga tgggtttctc       60

tatgtttata agggctatca acctatagat gtagttcgtg atctaccttc tggttttaac      120

actttgaaac ctatttttaa gttgcctctt ggtattaaca ttacaaattt tagagccatt      180

cttacagcct tttcacctgc tcaagacatt tggggcacgt cagctgcagc ctattttgtt      240

ggctatttaa agccaactac atttatgctc aagtatgatg aaaatggtac aatcacagat      300

gctgttgatt gttctcaaaa tccacttgct gaactcaaat gctctgttaa gagctttgag      360

attgacaaag gaatttacca gacctctaat ttcagggttg ttccctcagg agatgttgtg      420

agattcccta atattacaaa cttgtgtcct tttggagagg tttttaatgc tactaaattc      480

cc                                                                     482


<210>  22
<211>  496
<212>  DNA
<213>  SARS-CoV

<400>  22
catctaaaaa gcctcgccaa aaacgtactg ccacaaaaca gtacaacgtc actcaagcat       60

ttgggagacg tggtccagaa caaacccaag gaaatttcgg ggaccaagac ctaatcagac      120

aaggaactga ttacaaacat tggccgcaaa ttgcacaatt tgctccaagt gcctctgcat      180

tctttggaat gtcacgcatt ggcatggaag tcacaccttc gggaacatgg ctgacttatc      240

atggagccat taaattggat gacaaagatc cacaattcaa agacaacgtc atactgctga      300

acaagcacat tgacgcatac aaaacattcc caccaacaga gcctaaaaag gacaaaaaga      360

aaaagactga tgaagctcag cctttgccgc agagacaaaa gaagcagccc actgtgactc      420

ttcttcctgc ggctgacatg gatgatttct ccagacaact tcaaaattcc atgagtggag      480

cttctgctga ttcaac                                                      496


<210>  23
<211>  491
<212>  DNA
<213>  MERS-CoV

<400>  23
actctatgct aagcgtgctg tacgctcgca tcccgatttc aaattgctac acaatttaca       60

agcagacatt tgctacaagt tcgtcctttg ggattatgaa cgtagcaata tttatggtac      120

tgctactatt ggtgtatgta agtacactga tattgatgtt aattcagctt tgaatatatg      180

ttttgacata cgcgataatt gttcattgga gaagttcatg tctactccca atgccatctt      240

tatttctgat agaaaaatca agaaataccc ttgtatggta ggtcctgatt atgcttactt      300

caatggtgct atcatccgtg atagtgatgt tgttaaacaa ccagtgaagt tctacttgta      360

taagaaagtc aataatgagt ttattgatcc tactgagtgt atttacactc agagtcgctc      420

ttgtagtgac ttcctacccc tttctgacat ggagaaagac tttctatctt ttgatagtga      480

tgttttcatt a                                                           491


<210>  24
<211>  587
<212>  DNA
<213>  MERS-CoV

<400>  24
aattcctata cttcttttgc cacttatcac actcctgcaa cagattgttc tgatggcaat       60

tacaatcgta atgccagtct gaactctttt aaggagtatt ttaatttacg taactgcacc      120

tttatgtaca cttataacat taccgaagat gagattttag agtggtttgg cattacacaa      180

actgctcaag gtgttcacct cttctcatct cggtatgttg atttgtacgg cggcaatatg      240

tttcaatttg ccaccttgcc tgtttatgat actattaagt attattctat cattcctcac      300

agtattcgtt ctatccaaag tgatagaaaa gcttgggctg ccttctacgt atataaactt      360

caaccgttaa ctttcctgtt ggatttttct gttgatggtt atatacgcag agctatagac      420

tgtggtttta atgatttgtc acaactccac tgctcatatg aatccttcga tgttgaatct      480

ggagtttatt cagtttcgtc tttcgaagca aaaccttctg gctcagttgt ggaacaggct      540

gaaggtgttg aatgtgattt ttcacctctt ctgtctggca cacctcc                    587


<210>  25
<211>  484
<212>  DNA
<213>  MERS-CoV

<400>  25
ctaaaaataa gatgcgccac aagcgcactt ccaccaaaag tttcaacatg gtgcaagctt       60

ttggtcttcg cggaccagga gacctccagg gaaactttgg tgatcttcaa ttgaataaac      120

tcggcactga ggacccacgt tggccccaaa ttgctgagct tgctcctaca gccagtgctt      180

ttatgggtat gtcgcaattt aaacttaccc atcagaacaa tgatgatcat ggcaaccctg      240

tgtacttcct tcggtacagt ggagccatta aacttgaccc aaagaatccc aactacaata      300

agtggttgga gcttcttgag caaaatattg atgcctacaa aaccttccct aagaaggaaa      360

agaaacaaaa ggcaccaaaa gaagaatcaa cagaccaaat gtctgaacct ccaaaggagc      420

agcgtgtgca aggtagcatc actcagcgca ctcgcacccg tccaagtgtt cagcctggtc      480

caat                                                                   484


<210>  26
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  T7 promoter

<400>  26
taatacgact cactatagg                                                    19


<210>  27
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  27
taatacgact cactataggt aatttctact cttgtagat                              39


<210>  28
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  28
taatacgact cactataggt aatttctact aagtgtagat                             40


<210>  29
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  29
tgcaccactc actgtctttt                                                   20


<210>  30
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  30
aaaagacagt gagtggtgca atctacaaga gtagaaatta cctatagtga gtc              53


<210>  31
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  31
caaccatctg taggtcccaa                                                   20


<210>  32
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  32
ttgggaccta cagatggttg atctacaaga gtagaaatta cctatagtga gtc              53


<210>  33
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  33
cttgctttac atagaagtta                                                   20


<210>  34
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  34
taacttctat gtaaagcaag atctacaaga gtagaaatta cctatagtga gtc              53


<210>  35
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  35
actcctggtg attcttcttc                                                   20


<210>  36
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  36
gaagaagaat caccaggagt atctacaaga gtagaaatta cctatagtga gtc              53


<210>  37
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  37
gagtccaacc aacagaatct                                                   20


<210>  38
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  38
agattctgtt ggttggactc atctacaaga gtagaaatta cctatagtga gtc              53


<210>  39
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  39
aaactttact tgctttacat                                                   20


<210>  40
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  40
atgtaaagca agtaaagttt atctacaaga gtagaaatta cctatagtga gtc              53


<210>  41
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  41
catagaagtt atttgactcc                                                   20


<210>  42
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  42
ggagtcaaat aacttctatg atctacaaga gtagaaatta cctatagtga gtc              53


<210>  43
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  43
aaacctagtg atgttaatac                                                   20


<210>  44
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  44
gtattaacat cactaggttt atctacaaga gtagaaatta cctatagtga gtc              53


<210>  45
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  45
ccaataggta ttaacatcac                                                   20


<210>  46
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  46
gtgatgttaa tacctattgg atctacaaga gtagaaatta cctatagtga gtc              53


<210>  47
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  47
gaaccattgg tagatttgcc                                                   20


<210>  48
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  48
ggcaaatcta ccaatggttc atctacaaga gtagaaatta cctatagtga gtc              53


<210>  49
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  49
gaaccattgg tagatttgcc                                                   20


<210>  50
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  50
ctaccaatgg ttctaaagcc atctacaaga gtagaaatta cctatagtga gtc              53


<210>  51
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  51
atagaaaagt cctaggttga                                                   20


<210>  52
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  52
tcaacctagg acttttctat atctacaaga gtagaaatta cctatagtga gtc              53


<210>  53
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  53
tgagagaggg tcaagtgcac                                                   20


<210>  54
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  54
gtgcacttga ccctctctca atctacaaga gtagaaatta cctatagtga gtc              53


<210>  55
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  55
tttctgagag agggtcaagt                                                   20


<210>  56
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  56
acttgaccct ctctcagaaa atctacaaga gtagaaatta cctatagtga gtc              53


<210>  57
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  57
aacgtacact ttgtttctga                                                   20


<210>  58
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  58
tcagaaacaa agtgtacgtt atctacaaga gtagaaatta cctatagtga gtc              53


<210>  59
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  59
tacagtgaag gatttcaacg                                                   20


<210>  60
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  60
cgttgaaatc cttcactgta atctacaaga gtagaaatta cctatagtga gtc              53


<210>  61
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  61
gtgcgtgatc tccctcaggg                                                   20


<210>  62
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  62
ccctgaggga gatcacgcac atctacaaga gtagaaatta cctatagtga gtc              53


<210>  63
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  63
aaagatcaag tcattttgct                                                   20


<210>  64
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  64
agcaaaatga cttgatcttt atctacaaga gtagaaatta cctatagtga gtc              53


<210>  65
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  65
cccccagcgc ttcagcgttc                                                   20


<210>  66
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  66
gaacgctgaa gcgctggggg atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  67
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  67
tgcaccactc actgtctttt                                                   20


<210>  68
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  68
aaaagacagt gagtggtgca atctacactt agtagaaatt acctatagtg ag               52


<210>  69
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  69
caaccatctg taggtcccaa                                                   20


<210>  70
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  70
ttgggaccta cagatggttg atctacactt agtagaaatt acctatagtg ag               52


<210>  71
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  71
cttgctttac atagaagtta                                                   20


<210>  72
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  72
taacttctat gtaaagcaag atctacactt agtagaaatt acctatagtg ag               52


<210>  73
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  73
actcctggtg attcttcttc                                                   20


<210>  74
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  74
gaagaagaat caccaggagt atctacactt agtagaaatt acctatagtg ag               52


<210>  75
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  75
gagtccaacc aacagaatct                                                   20


<210>  76
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  76
agattctgtt ggttggactc atctacactt agtagaaatt acctatagtg ag               52


<210>  77
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  77
aaagatcaag tcattttgct                                                   20


<210>  78
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  78
agcaaaatga cttgatcttt atctacactt agtagaaatt acctatagtg ag               52


<210>  79
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  79
cccccagcgc ttcagcgttc                                                   20


<210>  80
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  80
gaacgctgaa gcgctggggg atctacactt agtagaaatt acctatagtg agtcg            55


<210>  81
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  81
actcctggtg attcttct                                                     18


<210>  82
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  82
agaagaatca ccaggagtat ctacaagagt agaaattacc tatagtgagt cg               52


<210>  83
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  83
actcctggtg attcttct                                                     18


<210>  84
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  84
agaagaatca ccaggagtat ctacacttag tagaaattac ctatagtgag tc               52


<210>  85
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  85
cccccagcgc ttcagcgt                                                     18


<210>  86
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  86
acgctgaagc gctgggggat ctacaagagt agaaattacc tatagtgagt                  50


<210>  87
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  87
cccccagcgc ttcagcgt                                                     18


<210>  88
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  88
acgctgaagc gctgggggat ctacacttag tagaaattac ctatagtgag                  50


<210>  89
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  89
actcctggtg attcttctt                                                    19


<210>  90
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  90
aagaagaatc accaggagta tctacaagag tagaaattac ctatagtgag                  50


<210>  91
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  91
actcctggtg attcttctt                                                    19


<210>  92
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  92
aagaagaatc accaggagta tctacactta gtagaaatta cctatagtga                  50


<210>  93
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  93
cccccagcgc ttcagcgtt                                                    19


<210>  94
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  94
aacgctgaag cgctggggga tctacaagag tagaaattac ctatagtgag tcgt             54


<210>  95
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  95
cccccagcgc ttcagcgtt                                                    19


<210>  96
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  96
aacgctgaag cgctggggga tctacactta gtagaaatta cctatagtga gtcg             54


<210>  97
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  97
accccagcgc ttcagcgttc                                                   20


<210>  98
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  98
gaacgctgaa gcgctggggt atctacaaga gtagaaatta cctatagtga                  50


<210>  99
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  99
ccaccagcgc ttcagcgttc                                                   20


<210>  100
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  100
gaacgctgaa gcgctggtgg atctacaaga gtagaaatta cctatagtga                  50


<210>  101
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  101
ccccaagcgc ttcagcgttc                                                   20


<210>  102
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  102
gaacgctgaa gcgcttgggg atctacaaga gtagaaatta cctatagtga                  50


<210>  103
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  103
cccccatcgc ttcagcgttc                                                   20


<210>  104
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  104
gaacgctgaa gcgatggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  105
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  105
cccccagctc ttcagcgttc                                                   20


<210>  106
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  106
gaacgctgaa gagctggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  107
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  107
cccccagcgc gtcagcgttc                                                   20


<210>  108
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  108
gaacgctgac gcgctggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  109
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  109
cccccagcgc ttaagcgttc                                                   20


<210>  110
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  110
gaacgcttaa gcgctggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  111
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  111
cccccagcgc ttcatcgttc                                                   20


<210>  112
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  112
gaacgatgaa gcgctggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  113
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  113
cccccagcgc ttcagcattc                                                   20


<210>  114
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  114
gaatgctgaa gcgctggggg atctacaaga gtagaaatta cctatagtga                  50


<210>  115
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  115
cccccagcgc ttcagcgtgc                                                   20


<210>  116
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  116
gcacgctgaa gcgctggggg atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  117
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  117
accccagcgc ttcagcgttc                                                   20


<210>  118
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  118
gaacgctgaa gcgctggggt atctacactt agtagaaatt acctatagtg                  50


<210>  119
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  119
ccaccagcgc ttcagcgttc                                                   20


<210>  120
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  120
gaacgctgaa gcgctggtgg atctacactt agtagaaatt acctatagtg                  50


<210>  121
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  121
ccccaagcgc ttcagcgttc                                                   20


<210>  122
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  122
gaacgctgaa gcgcttgggg atctacactt agtagaaatt acctatagtg                  50


<210>  123
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  123
cccccatcgc ttcagcgttc                                                   20


<210>  124
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  124
gaacgctgaa gcgatggggg atctacactt agtagaaatt acctatagtg                  50


<210>  125
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  125
cccccagctc ttcagcgttc                                                   20


<210>  126
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  126
gaacgctgaa gagctggggg atctacactt agtagaaatt acctatagtg                  50


<210>  127
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  127
cccccagcgc gtcagcgttc                                                   20


<210>  128
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  128
gaacgctgac gcgctggggg atctacactt agtagaaatt acctatagtg                  50


<210>  129
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  129
cccccagcgc ttaagcgttc                                                   20


<210>  130
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  130
gaacgcttaa gcgctggggg atctacactt agtagaaatt acctatagtg                  50


<210>  131
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  131
cccccagcgc ttcatcgttc                                                   20


<210>  132
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  132
gaacgatgaa gcgctggggg atctacactt agtagaaatt acctatagtg                  50


<210>  133
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  133
cccccagcgc ttcagcattc                                                   20


<210>  134
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  134
gaatgctgaa gcgctggggg atctacactt agtagaaatt acctatagtg                  50


<210>  135
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  135
cccccagcgc ttcagcgtgc                                                   20


<210>  136
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  136
gcacgctgaa gcgctggggg atctacactt agtagaaatt acctatagtg agtcg            55


<210>  137
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  137
cctcctggtg attcttcttc                                                   20


<210>  138
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  138
gaagaagaat caccaggagg atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  139
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  139
acgcctggtg attcttcttc                                                   20


<210>  140
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  140
gaagaagaat caccaggcgt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  141
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  141
actcatggtg attcttcttc                                                   20


<210>  142
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  142
gaagaagaat caccatgagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  143
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  143
actccttgtg attcttcttc                                                   20


<210>  144
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  144
gaagaagaat cacaaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  145
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  145
actcctgggg attcttcttc                                                   20


<210>  146
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  146
gaagaagaat ccccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  147
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  147
actcctggtg cttcttcttc                                                   20


<210>  148
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  148
gaagaagaag caccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  149
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  149
actcctggtg atgcttcttc                                                   20


<210>  150
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  150
gaagaagcat caccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  151
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  151
actcctggtg attcgtcttc                                                   20


<210>  152
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  152
gaagacgaat caccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  153
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  153
actcctggtg attcttattc                                                   20


<210>  154
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  154
gaataagaat caccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  155
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  155
actcctggtg attcttctgc                                                   20


<210>  156
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  156
gcagaagaat caccaggagt atctacaaga gtagaaatta cctatagtga gtcgt            55


<210>  157
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  157
cctcctggtg attcttcttc                                                   20


<210>  158
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  158
gaagaagaat caccaggagg atctacactt agtagaaatt acctatagtg agtcg            55


<210>  159
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  159
acgcctggtg attcttcttc                                                   20


<210>  160
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  160
gaagaagaat caccaggcgt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  161
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  161
actcatggtg attcttcttc                                                   20


<210>  162
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  162
gaagaagaat caccatgagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  163
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  163
actccttgtg attcttcttc                                                   20


<210>  164
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  164
gaagaagaat cacaaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  165
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  165
actcctgggg attcttcttc                                                   20


<210>  166
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  166
gaagaagaat ccccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  167
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  167
actcctggtg cttcttcttc                                                   20


<210>  168
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  168
gaagaagaag caccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  169
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  169
actcctggtg atgcttcttc                                                   20


<210>  170
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  170
gaagaagcat caccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  171
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  171
actcctggtg attcgtcttc                                                   20


<210>  172
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  172
gaagacgaat caccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  173
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  173
actcctggtg attcttattc                                                   20


<210>  174
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  174
gaataagaat caccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  175
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  175
actcctggtg attcttctgc                                                   20


<210>  176
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  176
gcagaagaat caccaggagt atctacactt agtagaaatt acctatagtg agtcg            55


<210>  177
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  177
tagtccaacc aacagaatct                                                   20


<210>  178
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  178
agattctgtt ggttggacta atctacaaga gtagaaatta cctatagtga                  50


<210>  179
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  179
gattccaacc aacagaatct                                                   20


<210>  180
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  180
agattctgtt ggttggaatc atctacaaga gtagaaatta cctatagtga                  50


<210>  181
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  181
gagtacaacc aacagaatct                                                   20


<210>  182
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  182
agattctgtt ggttgtactc atctacaaga gtagaaatta cctatagtga                  50


<210>  183
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  183
gagtcccacc aacagaatct                                                   20


<210>  184
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  184
agattctgtt ggtgggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  185
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  185
gagtccaaac aacagaatct                                                   20


<210>  186
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  186
agattctgtt gtttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  187
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  187
gagtccaacc cacagaatct                                                   20


<210>  188
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  188
agattctgtg ggttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  189
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  189
gagtccaacc aaaagaatct                                                   20


<210>  190
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  190
agattctttt ggttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  191
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  191
gagtccaacc aacataatct                                                   20


<210>  192
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  192
agattatgtt ggttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  193
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  193
gagtccaacc aacagactct                                                   20


<210>  194
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  194
agagtctgtt ggttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  195
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  195
gagtccaacc aacagaatat                                                   20


<210>  196
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  196
atattctgtt ggttggactc atctacaaga gtagaaatta cctatagtga                  50


<210>  197
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  197
tagtccaacc aacagaatct                                                   20


<210>  198
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  198
agattctgtt ggttggacta atctacactt agtagaaatt acctatagtg                  50


<210>  199
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  199
gattccaacc aacagaatct                                                   20


<210>  200
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  200
agattctgtt ggttggaatc atctacactt agtagaaatt acctatagtg                  50


<210>  201
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  201
gagtacaacc aacagaatct                                                   20


<210>  202
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  202
agattctgtt ggttgtactc atctacactt agtagaaatt acctatagtg                  50


<210>  203
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  203
gagtcccacc aacagaatct                                                   20


<210>  204
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  204
agattctgtt ggtgggactc atctacactt agtagaaatt acctatagtg                  50


<210>  205
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  205
gagtccaaac aacagaatct                                                   20


<210>  206
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  206
agattctgtt gtttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  207
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  207
gagtccaacc cacagaatct                                                   20


<210>  208
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  208
agattctgtg ggttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  209
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  209
gagtccaacc aaaagaatct                                                   20


<210>  210
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  210
agattctttt ggttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  211
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  211
gagtccaacc aacataatct                                                   20


<210>  212
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  212
agattatgtt ggttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  213
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  213
gagtccaacc aacagactct                                                   20


<210>  214
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  214
agagtctgtt ggttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  215
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  spacer

<400>  215
gagtccaacc aacagaatat                                                   20


<210>  216
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide

<400>  216
atattctgtt ggttggactc atctacactt agtagaaatt acctatagtg                  50


<210>  217
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  217
gctttgggct aagcgcaa                                                     18


<210>  218
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  218
aaacagggta atttcaaaaa tct                                               23


<210>  219
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  219
cttctaagaa gcctcggc                                                     18


<210>  220
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  220
gctttgggct aagcgtaa                                                     18


<210>  221
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  221
aagtcaggta attttaaaca cttac                                             25


<210>  222
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  222
catctaaaaa gcctcgcca                                                    19


<210>  223
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  223
actctatgct aagcgtgc                                                     18


<210>  224
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  224
aattcctata cttcttttgc cactt                                             25


<210>  225
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  225
ctaaaaataa gatgcgccac aag                                               23


<210>  226
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  226
caatgaattc atccatagct aattctaaga                                        30


<210>  227
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  227
tggcgttaaa aacttcacca                                                   20


<210>  228
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  228
gttgagtcag cactgctcat                                                   20


<210>  229
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  229
gtatgaattc atccatagcg ag                                                22


<210>  230
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  230
gggaatttag tagcattaaa aacc                                              24


<210>  231
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  231
gttgaatcag cagaagctcc                                                   20


<210>  232
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  232
taatgaaaac atcactatca aaagat                                            26


<210>  233
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  233
ggaggtgtgc cagacaga                                                     18


<210>  234
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  234
attggaccag gctgaacac                                                    19


<210>  235
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  235
taatacgact cactataggg ctttgggcta agcgcaa                                37


<210>  236
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  236
taatacgact cactatagga aacagggtaa tttcaaaaat ct                          42


<210>  237
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  237
taatacgact cactataggc ttctaagaag cctcggc                                37


<210>  238
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  238
aacacaagct ttcggcag                                                     18


<210>  239
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  239
gaaatttgga tctttgtcat cc                                                22


<210>  240
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  240
tgcggccaat gtttgtaatc agccaaggaa attttgggga c                           41


<210>  241
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  241
cgcattggca tggaagtcac tttgatggca cctgtgtag                              39


<210>  242
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  242
ttccttgtct gattagttc                                                    19


<210>  243
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  243
accttcggga acgtggtt                                                     18


<210>  244
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  244
ttaatttagt gcgtgatctc c                                                 21


<210>  245
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  245
agcatctgta atggttccat                                                   20


<210>  246
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  246
gcaagtaaag tttgaaacct agctcagggt ttttcggct                              39


<210>  247
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  247
aagcaagtaa agtttgaaac ctactcaggg tttttcggct                             40


<210>  248
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  248
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg ct                          42


<210>  249
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  249
tggacagctg gtgctaatag aaaagtccta ggttgaag                               38


<210>  250
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  250
ggcaaatcta ccaatggttc taa                                               23


<210>  251
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  251
gcagcttatt atgtgggtta t                                                 21


<210>  252
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  252
tgtaaagcaa gtaaagtttg aaacc                                             25


<210>  253
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  253
tggacagctg gtgct                                                        15


<210>  254
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  254
agaagttatt tgactcctgg tg                                                22


<210>  255
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  255
cctgaagaag aatcaccagg                                                   20


<210>  256
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  256
caccaggagt caaataactt ct                                                22


<210>  257
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  257
actcctggtg attcttcttc a                                                 21


<210>  258
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer sequence

<400>  258
taatttctac tcttgtagat                                                   20


<210>  259
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer sequence

<400>  259
aatttctact aagtgtagat                                                   20


<210>  260
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  260
ttaatttagt gcgtgatctc a                                                 21


<210>  261
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  261
ttaatttagt gcgtgatcta c                                                 21


<210>  262
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  262
ttaatttagt gcgtgatcgc c                                                 21


<210>  263
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  263
agcatctgta atggttccag                                                   20


<210>  264
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  264
agcatctgta atggttccct                                                   20


<210>  265
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  265
agcatctgta atggttcaat                                                   20


<210>  266
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  266
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg cg                          42


<210>  267
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  267
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg at                          42


<210>  268
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  268
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgt ct                          42


<210>  269
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  269
ggtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg ct                          42


<210>  270
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  270
tttaaagcaa gtaaagtttg aaaccctcag ggtttttcgg ct                          42


<210>  271
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  271
tggaaagcaa gtaaagtttg aaaccctcag ggtttttcgg ct                          42


<210>  272
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  272
tgtaaagcaa gtaaagtttg aaaccctcag ggtttttcgg c                           41


<210>  273
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  273
gtaaagcaag taaagtttga aaccctcagg gtttttcggc t                           41


<210>  274
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  274
tggacagctg gtgctaatag aaaagtccta ggttgaat                               38


<210>  275
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  275
tggacagctg gtgctaatag aaaagtccta ggttgacg                               38


<210>  276
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  276
tggacagctg gtgctaatag aaaagtccta ggttgcag                               38


<210>  277
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  277
gggacagctg gtgctaatag aaaagtccta ggttgaag                               38


<210>  278
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  278
ttgacagctg gtgctaatag aaaagtccta ggttgaag                               38


<210>  279
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  279
tgtacagctg gtgctaatag aaaagtccta ggttgaag                               38


<210>  280
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  280
tggacagctg gtgctaatag aaaagtccta ggttgaa                                37


<210>  281
<211>  40
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  281
uaauuucuac ucuuguagau acuccuggug auucuucuuc                             40


<210>  282
<211>  44
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  282
uggauaauuu cuacucuugu agauacuccu ggugauucuu cuuc                        44


<210>  283
<211>  49
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  283
gggaauggau aauuucuacu cuuguagaua cuccugguga uucuucuuc                   49


<210>  284
<211>  42
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  2'-O-methyl-C, phosphorothioate linkage

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  phosphorothioate linkage

<400>  284
cuuaauuucu acucuuguag auacuccugg ugauucuucu uc                          42


<210>  285
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  gRNA/DNA hybrid


<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  RNA nucleotides

<400>  285
uaauuucuac ucuuguagau acuccuggug auucuucutc                             40


<210>  286
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  rRNA/DNA hybrid


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  DNA nucleotide

<220>
<221>  misc_feature
<222>  (22)..(27)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  DNA nucleotide

<220>
<221>  misc_feature
<222>  (29)..(38)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  DNA nucleotide

<400>  286
uaauuucuac ucuuguagau acuccuggug auucuucutc                             40


<210>  287
<211>  40
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  287
uaauuucuac ucuuguagau aaaccuagug auguuaauac                             40


<210>  288
<211>  44
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  288
uggauaauuu cuacucuugu agauaaaccu agugauguua auac                        44


<210>  289
<211>  49
<212>  RNA
<213>  Artificial

<220>
<223>  gRNA

<400>  289
gggaauggau aauuucuacu cuuguagaua aaccuaguga uguuaauac                   49


<210>  290
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  gRNA/DNA hybrid


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  DNA nucleotide

<220>
<221>  misc_feature
<222>  (22)..(27)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  DNA nucleotide

<220>
<221>  misc_feature
<222>  (29)..(38)
<223>  RNA nucleotide

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  DNA nucleotide

<400>  290
uaauuucuac ucuuguagau aaaccuagug auguuaauac                             40


