                         SEQUENCE LISTING

<110>  PentaBase ApS
 
<120>  Detection of methylation status

<130>  P5659PC00

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  266
<212>  DNA
<213>  Homo sapiens

<400>  1
ctcttgcttt tctcaggtcc tcggctccgc cccgctctag accccgcccc acgccgccat       60

ccccgtgccc ctcggccccg cccccgcgcc ccggatatgc tgggacagcc cgcgccccta      120

gaacgctttg cgtcccgacg cccgcaggtc ctcgcggtgc gcaccgtttg cgacttggtg      180

agtgtctggg tcgcctcgct cccggaagag tgcggagctc tccctcggga cggtggcagc      240

ctcgagtggt cctgcaggcg ccctca                                           266


<210>  2
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Z Probe


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type Z

<400>  2
cngtcccnga cngcccng                                                     18


<210>  3
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E Probe


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  3
cngtcccnga cngcccng                                                     18


<210>  4
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E2 Probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Hydrophobic nucleotide of type E

<400>  4
ncgtccncga ncgccncg                                                     18


<210>  5
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E3 Probe


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Hydrophobic nucleotide of type E

<400>  5
cgntcccgna cgncccgn                                                     18


<210>  6
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Ref Probe


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Probe

<400>  6
cgtcccgacg cccg                                                         14


<210>  7
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_0mC Target


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Target

<400>  7
cgggcgtcgg gacg                                                         14


<210>  8
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_1,2,3,4mC Target


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Methylated nucleotide

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Methylated nucleotide

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Methylated nucleotide

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Methylated nucleotide

<400>  8
cgggcgtcgg gacg                                                         14


<210>  9
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_1mC Target


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Methylated nucleotide

<400>  9
cgggcgtcgg gacg                                                         14


<210>  10
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_2mC Target


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Methylated nucleotide

<400>  10
cgggcgtcgg gacg                                                         14


<210>  11
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_3mC Target


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Methylated nucleotide

<400>  11
cgggcgtcgg gacg                                                         14


<210>  12
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_4mC Target


<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Methylated nucleotide

<400>  12
cgggcgtcgg gacg                                                         14


<210>  13
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_2,3mC Target


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Methylated nucleotide

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Methylated nucleotide

<400>  13
cgggcgtcgg gacg                                                         14


<210>  14
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_FwLoop3B BasePrimer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  14
cngtcccnga cngcccngag gcttctgaca ggtcctcg                               38


<210>  15
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_FwLoop3C BasePrimer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  15
cngtcccnga cngcccngag gcttctgaca ggtcctcgcg                             40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_FwLoop4B BasePrimer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  16
cngtcccnga cngcccngag gcttcactga caggtcctcg                             40


<210>  17
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_FwLoop4C BasePrimer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  17
cngtcccnga cngcccngag gcttcactga caggtcctcg cg                          42


<210>  18
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Fw2C Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Primer

<400>  18
cgcccctaga acgctttgcg tccc                                              24


<210>  19
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Rev2C Primer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  Primer

<400>  19
ggagcgaggc gacccagaca ctca                                              24


<210>  20
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Probe2A


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Hydrophobic nucleotide of type Z

<400>  20
caagtcngca aacnggtng                                                    19


<210>  21
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Probe2B


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Hydrophobic nucleotide of type Z

<400>  21
caagtcgcna aacnggtng                                                    19


<210>  22
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Probe2D


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Hydrophobic nucleotide of type Z

<400>  22
caagtcgcna aacnggtgcn g                                                 21


<210>  23
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Probe2E


<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (14)..(14)
<223>  Hydrophobic nucleotide of type Z

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Hydrophobic nucleotide of type Z

<400>  23
caagtcgcna aacnggtgcn gcac                                              24


<210>  24
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_FwLoop5C BasePrimer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  24
cngtcccnga cngcccngag cgcttcactg agacaggtcc tcgcg                       45


<210>  25
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Fw2C_suE Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Hydrophobic nucleotide of type E

<400>  25
ncgcccctag aacgctttgc gtccc                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_Rev2C_suE Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Hydrophobic nucleotide of type E

<400>  26
nggagcgagg cgacccagac actca                                             25


<210>  27
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E_M1


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  27
cngacccngt cngcccng                                                     18


<210>  28
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E_M2


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  28
cngtcacnga cngcacng                                                     18


<210>  29
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E_M3


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  29
cngtcacnga cngcccng                                                     18


<210>  30
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E_M4


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  30
cngtcccnga cngcacng                                                     18


<210>  31
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  MGMT_E_M5


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  31
cngacccnga cngcccng                                                     18


<210>  32
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5D_M5


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  32
cngacccnga cngcccngag cgcttcactg agacaggtcc tcgc                        44


<210>  33
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_Rev2E

<400>  33
gagcgaggcg acccagacac tca                                               23


<210>  34
<211>  43
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  34
cngacccnga cngcccngag cgcttcactg agacaggtcc tcg                         43


<210>  35
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_2A


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  35
cngacccnga cngcccngag cgcttcactg agaggtcctc gc                          42


<210>  36
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_2B


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  36
cngacccnga cngcccngag cgcttcactg agaggtcctc g                           41


<210>  37
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_4A


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  37
cngacccnga cngcccngag cgcttcactg agatcctcgc gg                          42


<210>  38
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_4B


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  38
cngacccnga cngcccngag cgcttcactg agatcctcgc g                           41


<210>  39
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_6A


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  39
cngacccnga cngcccngag cgcttcactg agactcgcgg t                           41


<210>  40
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_6B


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  40
cngacccnga cngcccngag cgcttcactg agactcgcgg                             40


<210>  41
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_8A


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  41
cngacccnga cngcccngag cgcttcactg agacgcggt                              39


<210>  42
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  MGMT_FwLoop5B_M5_8B


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Hydrophobic nucleotide of type E

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Hydrophobic nucleotide of type E

<400>  42
cngacccnga cngcccngag cgcttcactg agacgcggtg                             40


