                         SEQUENCE LISTING

<110>  GEORGIA TECH RESEARCH CORPORATION et al.
 
<120>  CROSSLINKED HYDROGEL FOR IMMUNE CHECKPOINT BLOCKADE DELIVERY

<130>  10034-110WO1

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1
agatctgcgt ttactatcac ggctccaaag gacttgtacg tggtggagta tggcagcaac       60

gtcacgatgg agtgcagatt ccctgtagaa cgggagctgg acctgcttgc gttagtggtg      120

tactgggaaa aggaagatga gcaagtgatt cagtttgtgg caggagagga ggaccttaag      180

cctcagcaca gcaacttcag ggggagagcc tcgctgccaa aggaccagct tttgaaggga      240

aatgctgccc ttcagatcac agacgtcaag ctgcaggacg caggcgttta ctgctgcata      300

atcagctacg gtggtgcgga ctacaagcga atcacgctga aagtcaatgc cccataccgc      360

aaaatcaacc agagaatttc cgtggatcca gccacttctg agcatgaact aatatgtcag      420

gccgagggtt atccagaagc tgaggtaatc tggacaaaca gtgaccacca acccgtgagt      480

gggaagagaa gtgtcaccac ttcccggaca gaggggatgc ttctcaatgt gaccagcagt      540

ctgagggtca acgccacagc gaatgatgtt ttctactgta cgttttggag atcacagcca      600

gggcaaaacc acacagcgga gctgatcatc ccagaactgc ctgcaacaca tcctccacag      660

aacaggagat ctgctgaagc tgcagctaaa gaagctgcag ctaaagctgc tgctggtacc      720

atcaccggca cctggtacaa ccagctcggc tcgaccttca tcgtgaccgc gggcgccgat      780

ggcgccctga ccggaaccta cgagtcggcc gtcggcaacg ccgagagccg ctacgtcctg      840

accggtcgtt acgacagcgc cccggccacc gacggcagcg gcaccgccct cggttggacg      900

gtggcctgga agaataacta ccgcaacgcc cactccgcga ccacgtggag cggccagtac      960

gtcggcggcg ccgaggcgag gatcaacacc cagtggctgt tgacctccgg cgccaccgag     1020

gccaacgcct ggaagtccac gctggtcggc cacgacacct tcaccaaggt gaagccgtcc     1080

gccgcctcaa gcggaggcgg tggatcaggt ggaggcgaat tccatcatca ccatcaccat     1140

taatagctcg agtaatagct cgag                                            1164


<210>  2
<211>  380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Arg Ser Ala Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu 
1               5                   10                  15      


Tyr Gly Ser Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu 
            20                  25                  30          


Leu Asp Leu Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln 
        35                  40                  45              


Val Ile Gln Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser 
    50                  55                  60                  


Asn Phe Arg Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly 
65                  70                  75                  80  


Asn Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val 
                85                  90                  95      


Tyr Cys Cys Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr 
            100                 105                 110         


Leu Lys Val Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val 
        115                 120                 125             


Asp Pro Ala Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr 
    130                 135                 140                 


Pro Glu Ala Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser 
145                 150                 155                 160 


Gly Lys Arg Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn 
                165                 170                 175     


Val Thr Ser Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr 
            180                 185                 190         


Cys Thr Phe Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu 
        195                 200                 205             


Ile Ile Pro Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg Arg Ser 
    210                 215                 220                 


Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ala Gly Thr 
225                 230                 235                 240 


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
                245                 250                 255     


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
            260                 265                 270         


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
        275                 280                 285             


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
    290                 295                 300                 


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
305                 310                 315                 320 


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
                325                 330                 335     


Gly Ala Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
            340                 345                 350         


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ser Gly Gly Gly Gly 
        355                 360                 365             


Ser Gly Gly Gly Glu Phe His His His His His His 
    370                 375                 380 


<210>  3
<211>  219
<212>  PRT
<213>  Mouse

<400>  3

Phe Thr Ile Thr Ala Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly Ser 
1               5                   10                  15      


Asn Val Thr Met Glu Cys Arg Phe Pro Val Glu Arg Glu Leu Asp Leu 
            20                  25                  30          


Leu Ala Leu Val Val Tyr Trp Glu Lys Glu Asp Glu Gln Val Ile Gln 
        35                  40                  45              


Phe Val Ala Gly Glu Glu Asp Leu Lys Pro Gln His Ser Asn Phe Arg 
    50                  55                  60                  


Gly Arg Ala Ser Leu Pro Lys Asp Gln Leu Leu Lys Gly Asn Ala Ala 
65                  70                  75                  80  


Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Cys Cys 
                85                  90                  95      


Ile Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Leu Lys Val 
            100                 105                 110         


Asn Ala Pro Tyr Arg Lys Ile Asn Gln Arg Ile Ser Val Asp Pro Ala 
        115                 120                 125             


Thr Ser Glu His Glu Leu Ile Cys Gln Ala Glu Gly Tyr Pro Glu Ala 
    130                 135                 140                 


Glu Val Ile Trp Thr Asn Ser Asp His Gln Pro Val Ser Gly Lys Arg 
145                 150                 155                 160 


Ser Val Thr Thr Ser Arg Thr Glu Gly Met Leu Leu Asn Val Thr Ser 
                165                 170                 175     


Ser Leu Arg Val Asn Ala Thr Ala Asn Asp Val Phe Tyr Cys Thr Phe 
            180                 185                 190         


Trp Arg Ser Gln Pro Gly Gln Asn His Thr Ala Glu Leu Ile Ile Pro 
        195                 200                 205             


Glu Leu Pro Ala Thr His Pro Pro Gln Asn Arg 
    210                 215                 


<210>  4
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4

Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
1               5                   10                  15      


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
            20                  25                  30          


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
        35                  40                  45              


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
    50                  55                  60                  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
65                  70                  75                  80  


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
                85                  90                  95      


Gly Ala Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
            100                 105                 110         


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ser 
        115                 120                 


<210>  5
<211>  1146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5
agatctttta ctgtcacggt tcccaaggac ctatatgtgg tagagtatgg tagcaatatg       60

acaattgaat gcaaattccc agtagaaaaa caattagacc tggctgcact aattgtctat      120

tgggaaatgg aggataagaa cattattcaa tttgtgcatg gagaggaaga cctgaaggtt      180

cagcatagta gctacagaca gagggcccgg ctgttgaagg accagctctc cctgggaaat      240

gctgcacttc agatcacaga tgtgaaattg caggatgcag gggtgtaccg ctgcatgatc      300

agctatggtg gtgccgacta caagcgaatt actgtgaaag tcaatgcccc atacaacaaa      360

atcaaccaaa gaattttggt tgtggatcca gtcacctctg aacatgaact gacatgtcag      420

gctgagggct accccaaggc cgaagtcatc tggacaagca gtgaccatca agtcctgagt      480

ggtaagacca ccaccaccaa ttccaagaga gaggagaagc ttttcaatgt gaccagcaca      540

ctgagaatca acacaacaac taatgagatt ttctactgca cttttaggag attagatcct      600

gaggaaaacc atacagctga attggtcatc ccagaactac ctctggcaca tcctccaaat      660

gaaagggctg aagctgcagc taaagaagct gcagctaaag ctgctgctgg taccatcacc      720

ggcacctggt acaaccagct cggctcgacc ttcatcgtga ccgcgggcgc cgatggcgcc      780

ctgaccggaa cctacgagtc ggccgtcggc aacgccgaga gccgctacgt cctgaccggt      840

cgttacgaca gcgccccggc caccgacggc agcggcaccg ccctcggttg gacggtggcc      900

tggaagaata actaccgcaa cgcccactcc gcgaccacgt ggagcggcca gtacgtcggc      960

ggcgccgagg cgaggatcaa cacccagtgg ctgttgacct ccggcgccac cgaggccaac     1020

gcctggaagt ccacgctggt cggccacgac accttcacca aggtgaagcc gtccgccgcc     1080

tcaagcggag gcggtggatc aggtggaggc gaattccatc atcaccatca ccattaatag     1140

ctcgag                                                                1146


<210>  6
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6

Arg Ser Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr 
1               5                   10                  15      


Gly Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu 
            20                  25                  30          


Asp Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile 
        35                  40                  45              


Ile Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser 
    50                  55                  60                  


Tyr Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn 
65                  70                  75                  80  


Ala Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr 
                85                  90                  95      


Arg Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val 
            100                 105                 110         


Lys Val Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val 
        115                 120                 125             


Asp Pro Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr 
    130                 135                 140                 


Pro Lys Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser 
145                 150                 155                 160 


Gly Lys Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn 
                165                 170                 175     


Val Thr Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr 
            180                 185                 190         


Cys Thr Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu 
        195                 200                 205             


Val Ile Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Ala Glu 
    210                 215                 220                 


Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Ala Ala Gly Thr Ile Thr 
225                 230                 235                 240 


Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr Ala Gly 
                245                 250                 255     


Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly Asn Ala 
            260                 265                 270         


Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro Ala Thr 
        275                 280                 285             


Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys Asn Asn 
    290                 295                 300                 


Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr Val Gly 
305                 310                 315                 320 


Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser Gly Ala 
                325                 330                 335     


Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp Thr Phe 
            340                 345                 350         


Thr Lys Val Lys Pro Ser Ala Ala Ser Ser Gly Gly Gly Gly Ser Gly 
        355                 360                 365             


Gly Gly Glu Phe His His His His His His 
    370                 375             


<210>  7
<211>  220
<212>  PRT
<213>  Homo sapiens

<400>  7

Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly Ser 
1               5                   10                  15      


Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu Asp Leu 
            20                  25                  30          


Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile Gln 
        35                  40                  45              


Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser Tyr Arg 
    50                  55                  60                  


Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn Ala Ala 
65                  70                  75                  80  


Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg Cys 
                85                  90                  95      


Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val Lys Val 
            100                 105                 110         


Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val Asp Pro 
        115                 120                 125             


Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr Pro Lys 
    130                 135                 140                 


Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser Gly Lys 
145                 150                 155                 160 


Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn Val Thr 
                165                 170                 175     


Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr Cys Thr 
            180                 185                 190         


Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu Val Ile 
        195                 200                 205             


Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg 
    210                 215                 220 


