﻿                         序列表

<110>  凯莱英生命科学技术（天津）有限公司

<120>  PVA膜固定化酶及其制备方法

<130>  PN109064

<160>  5

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  459
<212>  PRT
<213>  紫色色杆菌DSM30191(Chromobacterium violaceum DSM30191)

<400>  1
Met Gln Lys Gln Arg Thr Thr Ser Gln Trp Arg Glu Leu Asp Ala Ala 
1               5                   10                  15      
His His Leu His Pro Phe Thr Asp Thr Ala Ser Leu Asn Gln Ala Gly 
            20                  25                  30          
Ala Arg Val Met Thr Arg Gly Glu Gly Val Tyr Leu Trp Asp Ser Glu 
        35                  40                  45              
Gly Asn Lys Ile Ile Asp Gly Met Ala Gly Leu Trp Cys Val Asn Val 
    50                  55                  60                  
Gly Tyr Gly Arg Lys Asp Phe Ala Glu Ala Ala Arg Arg Gln Met Glu 
65                  70                  75                  80  
Glu Leu Pro Phe Tyr Asn Thr Phe Phe Lys Thr Thr His Pro Ala Val 
                85                  90                  95      
Val Glu Leu Ser Ser Leu Leu Ala Glu Val Thr Pro Ala Gly Phe Asp 
            100                 105                 110         
Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ser Val Asp Thr Met Ile 
        115                 120                 125             
Arg Met Val Arg Arg Tyr Trp Asp Val Gln Gly Lys Pro Glu Lys Lys 
    130                 135                 140                 
Thr Leu Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr Ile Gly Gly 
145                 150                 155                 160 
Ala Ser Leu Gly Gly Met Lys Tyr Met His Glu Gln Gly Asp Leu Pro 
                165                 170                 175     
Ile Pro Gly Met Ala His Ile Glu Gln Pro Trp Trp Tyr Lys His Gly 
            180                 185                 190         
Lys Asp Met Thr Pro Asp Glu Phe Gly Val Val Ala Ala Arg Trp Leu 
        195                 200                 205             
Glu Glu Lys Ile Leu Glu Ile Gly Ala Asp Lys Val Ala Ala Phe Val 
    210                 215                 220                 
Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Ala Thr 
225                 230                 235                 240 
Tyr Trp Pro Glu Ile Glu Arg Ile Cys Arg Lys Tyr Asp Val Leu Leu 
                245                 250                 255     
Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe 
            260                 265                 270         
Gly His Gln His Phe Gly Phe Gln Pro Asp Leu Phe Thr Ala Ala Lys 
        275                 280                 285             
Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Phe Val Gly Lys 
    290                 295                 300                 
Arg Val Ala Glu Gly Leu Ile Ala Gly Gly Asp Phe Asn His Gly Phe 
305                 310                 315                 320 
Thr Tyr Ser Gly His Pro Val Cys Ala Ala Val Ala His Ala Asn Val 
                325                 330                 335     
Ala Ala Leu Arg Asp Glu Gly Ile Val Gln Arg Val Lys Asp Asp Ile 
            340                 345                 350         
Gly Pro Tyr Met Gln Lys Arg Trp Arg Glu Thr Phe Ser Arg Phe Glu 
        355                 360                 365             
His Val Asp Asp Val Arg Gly Val Gly Met Val Gln Ala Phe Thr Leu 
    370                 375                 380                 
Val Lys Asn Lys Ala Lys Arg Glu Leu Phe Pro Asp Phe Gly Glu Ile 
385                 390                 395                 400 
Gly Thr Leu Cys Arg Asp Ile Phe Phe Arg Asn Asn Leu Ile Met Arg 
                405                 410                 415     
Ala Cys Gly Asp His Ile Val Ser Ala Pro Pro Leu Val Met Thr Arg 
            420                 425                 430         
Ala Glu Val Asp Glu Met Leu Ala Val Ala Glu Arg Cys Leu Glu Glu 
        435                 440                 445             
Phe Glu Gln Thr Leu Lys Ala Arg Gly Leu Ala 
    450                 455                 

<210>  2
<211>  476
<212>  PRT
<213>  柠檬节杆菌(Arthrobacter citreus)

<400>  2
Met Gly Leu Thr Val Gln Lys Ile Asn Trp Glu Gln Val Lys Glu Trp 
1               5                   10                  15      
Asp Arg Lys Tyr Leu Met Arg Thr Phe Ser Thr Gln Asn Glu Tyr Gln 
            20                  25                  30          
Pro Val Pro Ile Glu Ser Thr Glu Gly Asp Tyr Leu Ile Thr Pro Gly 
        35                  40                  45              
Gly Thr Arg Leu Leu Asp Phe Phe Asn Gln Leu Cys Cys Val Asn Leu 
    50                  55                  60                  
Gly Gln Lys Asn Gln Lys Val Asn Ala Ala Ile Lys Glu Ala Leu Asp 
65                  70                  75                  80  
Arg Tyr Gly Phe Val Trp Asp Thr Tyr Ala Thr Asp Tyr Lys Ala Lys 
                85                  90                  95      
Ala Ala Lys Ile Ile Ile Glu Asp Ile Leu Gly Asp Glu Asp Trp Pro 
            100                 105                 110         
Gly Lys Val Arg Phe Val Ser Thr Gly Ser Glu Ala Val Glu Thr Ala 
        115                 120                 125             
Leu Asn Ile Ala Arg Leu Tyr Thr Asn Arg Pro Leu Val Val Thr Arg 
    130                 135                 140                 
Glu His Asp Tyr His Gly Trp Thr Gly Gly Ala Ala Thr Val Thr Arg 
145                 150                 155                 160 
Leu Arg Ser Phe Arg Ser Gly Leu Val Gly Glu Asn Ser Glu Ser Phe 
                165                 170                 175     
Ser Ala Gln Ile Pro Gly Ser Ser Cys Ser Ser Ala Val Leu Met Ala 
            180                 185                 190         
Pro Ser Ser Asn Thr Phe Gln Asp Ser Asn Gly Asn Tyr Leu Lys Asp 
        195                 200                 205             
Glu Asn Gly Glu Leu Leu Ser Val Lys Tyr Thr Arg Arg Met Ile Glu 
    210                 215                 220                 
Asn Tyr Gly Pro Glu Gln Val Ala Ala Val Ile Thr Glu Val Ser Gln 
225                 230                 235                 240 
Gly Val Gly Ser Thr Met Pro Pro Tyr Glu Tyr Val Pro Gln Ile Arg 
                245                 250                 255     
Lys Met Thr Lys Glu Leu Gly Val Leu Trp Ile Ser Asp Glu Val Leu 
            260                 265                 270         
Thr Gly Phe Gly Arg Thr Gly Lys Trp Phe Gly Tyr Gln His Tyr Gly 
        275                 280                 285             
Val Gln Pro Asp Ile Ile Thr Met Gly Lys Gly Leu Ser Ser Ser Ser 
    290                 295                 300                 
Leu Pro Ala Gly Ala Val Val Val Ser Lys Glu Ile Ala Ala Phe Met 
305                 310                 315                 320 
Asp Lys His Arg Trp Glu Ser Val Ser Thr Tyr Ala Gly His Pro Val 
                325                 330                 335     
Ala Met Ala Ala Val Cys Ala Asn Leu Glu Val Met Met Glu Glu Asn 
            340                 345                 350         
Leu Val Glu Gln Ala Lys Asn Ser Gly Glu Tyr Ile Arg Ser Lys Leu 
        355                 360                 365             
Glu Leu Leu Gln Glu Lys His Lys Ser Ile Gly Asn Phe Asp Gly Tyr 
    370                 375                 380                 
Gly Leu Leu Trp Ile Val Asp Ile Val Asn Ala Lys Thr Lys Thr Pro 
385                 390                 395                 400 
Tyr Val Lys Leu Asp Arg Asn Phe Arg His Gly Met Asn Pro Asn Gln 
                405                 410                 415     
Ile Pro Thr Gln Ile Ile Met Glu Lys Ala Leu Glu Lys Gly Val Leu 
            420                 425                 430         
Ile Gly Gly Ala Met Pro Asn Thr Met Arg Ile Gly Ala Ser Leu Asn 
        435                 440                 445             
Val Ser Arg Gly Asp Ile Asp Lys Ala Met Asp Ala Leu Asp Tyr Ala 
    450                 455                 460                 
Leu Asp Tyr Leu Glu Ser Gly Glu Trp Gln Gln Ser 
465                 470                 475     

<210>  3
<211>  253
<212>  PRT
<213>  醋酸杆菌属CCTCC M20906(Acetobacter sp. CCTCC M209061)

<400>  3
Met Ala Arg Val Ala Gly Lys Val Ala Ile Val Ser Gly Ala Ala Asn 
1               5                   10                  15      
Gly Ile Gly Lys Ala Thr Ala Gln Leu Leu Ala Lys Glu Gly Ala Lys 
            20                  25                  30          
Val Val Ile Gly Asp Leu Lys Glu Glu Asp Gly Gln Lys Ala Val Ala 
        35                  40                  45              
Glu Ile Lys Ala Ala Gly Gly Glu Ala Ala Phe Val Lys Leu Asn Val 
    50                  55                  60                  
Thr Asp Glu Ala Ala Trp Lys Ala Ala Ile Gly Gln Thr Leu Lys Leu 
65                  70                  75                  80  
Tyr Gly Arg Leu Asp Ile Ala Val Asn Asn Ala Gly Ile Asn Tyr Ser 
                85                  90                  95      
Gly Ser Val Glu Ser Thr Ser Leu Glu Asp Trp Arg Arg Val Gln Ser 
            100                 105                 110         
Ile Asn Leu Asp Gly Val Phe Leu Gly Thr Gln Val Ala Ile Glu Ala 
        115                 120                 125             
Met Lys Lys Ser Gly Gly Gly Ser Ile Val Asn Leu Ser Ser Ile Ser 
    130                 135                 140                 
Gly Leu Ile Gly Asp Pro Met Leu Ala Ala Tyr Val Ala Ser Lys Gly 
145                 150                 155                 160 
Gly Val Arg Leu Phe Thr Lys Ser Ala Ala Leu His Cys Ala Lys Ser 
                165                 170                 175     
Gly Tyr Lys Ile Arg Val Asn Ser Val His Pro Gly Tyr Ile Trp Thr 
            180                 185                 190         
Pro Met Val Ala Gly Leu Thr Lys Glu Asp Ala Ala Ala Arg Gln Lys 
        195                 200                 205             
Leu Val Asp Leu His Pro Ile Gly His Leu Gly Glu Pro Asn Asp Ile 
    210                 215                 220                 
Ala Tyr Gly Ile Leu Tyr Leu Ala Ser Asp Glu Ser Lys Phe Val Thr 
225                 230                 235                 240 
Gly Ser Glu Leu Val Ile Asp Gly Gly Tyr Thr Ala Gln 
                245                 250             

<210>  4
<211>  541
<212>  PRT
<213>  红球菌属 Phi1(Rhodococcus sp. Phi1)

<400>  4
Met Thr Ala Gln Ile Ser Pro Thr Val Val Asp Ala Val Val Ile Gly 
1               5                   10                  15      
Ala Gly Phe Gly Gly Ile Tyr Ala Val His Lys Leu His Asn Glu Gln 
            20                  25                  30          
Gly Leu Thr Val Val Gly Phe Asp Lys Ala Asp Gly Pro Gly Gly Thr 
        35                  40                  45              
Trp Tyr Trp Asn Arg Tyr Pro Gly Ala Leu Ser Asp Thr Glu Ser His 
    50                  55                  60                  
Leu Tyr Arg Phe Ser Phe Asp Arg Asp Leu Leu Gln Asp Gly Thr Trp 
65                  70                  75                  80  
Lys Thr Thr Tyr Ile Thr Gln Pro Glu Ile Leu Glu Tyr Leu Glu Ser 
                85                  90                  95      
Val Val Asp Arg Phe Asp Leu Arg Arg His Phe Arg Phe Gly Thr Glu 
            100                 105                 110         
Val Thr Ser Ala Ile Tyr Leu Glu Asp Glu Asn Leu Trp Glu Val Ser 
        115                 120                 125             
Thr Asp Lys Gly Glu Val Tyr Arg Ala Lys Tyr Val Val Asn Ala Val 
    130                 135                 140                 
Gly Leu Leu Ser Ala Ile Asn Phe Pro Asp Leu Pro Gly Leu Asp Thr 
145                 150                 155                 160 
Phe Glu Gly Glu Thr Ile His Thr Ala Ala Trp Pro Glu Gly Lys Asn 
                165                 170                 175     
Leu Ala Gly Lys Arg Val Gly Val Ile Gly Thr Gly Ser Thr Gly Gln 
            180                 185                 190         
Gln Val Ile Thr Ala Leu Ala Pro Glu Val Glu His Leu Thr Val Phe 
        195                 200                 205             
Val Arg Thr Pro Gln Tyr Ser Val Pro Val Gly Asn Arg Pro Val Thr 
    210                 215                 220                 
Lys Glu Gln Ile Asp Ala Ile Lys Ala Asp Tyr Asp Gly Ile Trp Asp 
225                 230                 235                 240 
Ser Val Lys Lys Ser Ala Val Ala Phe Gly Phe Glu Glu Ser Thr Leu 
                245                 250                 255     
Pro Ala Met Ser Val Ser Glu Glu Glu Arg Asn Arg Ile Phe Gln Glu 
            260                 265                 270         
Ala Trp Asp His Gly Gly Gly Phe Arg Phe Met Phe Gly Thr Phe Gly 
        275                 280                 285             
Asp Ile Ala Thr Asp Glu Ala Ala Asn Glu Ala Ala Ala Ser Phe Ile 
    290                 295                 300                 
Arg Ser Lys Ile Ala Glu Ile Ile Glu Asp Pro Glu Thr Ala Arg Lys 
305                 310                 315                 320 
Leu Met Pro Thr Gly Leu Tyr Ala Lys Arg Pro Leu Cys Asp Asn Gly 
                325                 330                 335     
Tyr Tyr Glu Val Tyr Asn Arg Pro Asn Val Glu Ala Val Ala Ile Lys 
            340                 345                 350         
Glu Asn Pro Ile Arg Glu Val Thr Ala Lys Gly Val Val Thr Glu Asp 
        355                 360                 365             
Gly Val Leu His Glu Leu Asp Val Leu Val Phe Ala Thr Gly Phe Asp 
    370                 375                 380                 
Ala Val Asp Gly Asn Tyr Arg Arg Ile Glu Ile Arg Gly Arg Asn Gly 
385                 390                 395                 400 
Leu His Ile Asn Asp His Trp Asp Gly Gln Pro Thr Ser Tyr Leu Gly 
                405                 410                 415     
Val Thr Thr Ala Asn Phe Pro Asn Trp Phe Met Val Leu Gly Pro Asn 
            420                 425                 430         
Gly Pro Phe Thr Asn Leu Pro Pro Ser Ile Glu Thr Gln Val Glu Trp 
        435                 440                 445             
Ile Ser Asp Thr Val Ala Tyr Ala Glu Arg Asn Glu Ile Arg Ala Ile 
    450                 455                 460                 
Glu Pro Thr Pro Glu Ala Glu Glu Glu Trp Thr Gln Thr Cys Thr Asp 
465                 470                 475                 480 
Ile Ala Asn Ala Thr Leu Phe Thr Arg Gly Asp Ser Trp Ile Phe Gly 
                485                 490                 495     
Ala Asn Val Pro Gly Lys Lys Pro Ser Val Leu Phe Tyr Leu Gly Gly 
            500                 505                 510         
Leu Gly Asn Tyr Arg Asn Val Leu Ala Gly Val Val Ala Asp Ser Tyr 
        515                 520                 525             
Arg Gly Phe Glu Leu Lys Ser Ala Val Pro Val Thr Ala 
    530                 535                 540     

<210>  5
<211>  603
<212>  PRT
<213>  红球菌属 红色-SD-1(Rhodococcus ruber-SD1)

<400>  5
Met Thr Thr Ser Ile Asp Arg Glu Ala Leu Arg Arg Lys Tyr Ala Glu 
1               5                   10                  15      
Glu Arg Asp Lys Arg Ile Arg Pro Asp Gly Asn Asp Gln Tyr Ile Arg 
            20                  25                  30          
Leu Asp His Val Asp Gly Trp Ser His Asp Pro Tyr Met Pro Ile Thr 
        35                  40                  45              
Pro Arg Glu Pro Lys Leu Asp His Val Thr Phe Ala Phe Ile Gly Gly 
    50                  55                  60                  
Gly Phe Ser Gly Leu Val Thr Ala Ala Arg Leu Arg Glu Ser Gly Val 
65                  70                  75                  80  
Glu Ser Val Arg Ile Ile Asp Lys Ala Gly Asp Phe Gly Gly Val Trp 
                85                  90                  95      
Tyr Trp Asn Arg Tyr Pro Gly Ala Met Cys Asp Thr Ala Ala Met Val 
            100                 105                 110         
Tyr Met Pro Leu Leu Glu Glu Thr Gly Tyr Met Pro Thr Glu Lys Tyr 
        115                 120                 125             
Ala His Gly Pro Glu Ile Leu Glu His Cys Gln Arg Ile Gly Lys His 
    130                 135                 140                 
Tyr Asp Leu Tyr Asp Asp Ala Leu Phe His Thr Glu Val Thr Asp Leu 
145                 150                 155                 160 
Val Trp Gln Glu His Asp Gln Arg Trp Arg Ile Ser Thr Asn Arg Gly 
                165                 170                 175     
Asp His Phe Thr Ala Gln Phe Val Gly Met Gly Thr Gly Pro Leu His 
            180                 185                 190         
Val Ala Gln Leu Pro Gly Ile Pro Gly Ile Glu Ser Phe Arg Gly Lys 
        195                 200                 205             
Ser Phe His Thr Ser Arg Trp Asp Tyr Asp Tyr Thr Gly Gly Asp Ala 
    210                 215                 220                 
Leu Gly Ala Pro Met Asp Lys Leu Ala Asp Lys Arg Val Ala Val Ile 
225                 230                 235                 240 
Gly Thr Gly Ala Thr Ala Val Gln Cys Val Pro Glu Leu Ala Lys Tyr 
                245                 250                 255     
Cys Arg Glu Leu Tyr Val Val Gln Arg Thr Pro Ser Ala Val Asp Glu 
            260                 265                 270         
Arg Gly Asn His Pro Ile Asp Glu Lys Trp Phe Ala Gln Ile Ala Thr 
        275                 280                 285             
Pro Gly Trp Gln Lys Arg Trp Leu Asp Ser Phe Thr Ala Ile Trp Asp 
    290                 295                 300                 
Gly Val Leu Thr Asp Pro Ser Glu Leu Ala Ile Glu His Glu Asp Leu 
305                 310                 315                 320 
Val Gln Asp Gly Trp Thr Ala Leu Gly Gln Arg Met Arg Ala Ala Val 
                325                 330                 335     
Gly Ser Val Pro Ile Glu Gln Tyr Ser Pro Glu Asn Val Gln Arg Ala 
            340                 345                 350         
Leu Glu Glu Ala Asp Asp Glu Gln Met Glu Arg Ile Arg Ala Arg Val 
        355                 360                 365             
Asp Glu Ile Val Thr Asp Pro Ala Thr Ala Ala Gln Leu Lys Ala Trp 
    370                 375                 380                 
Phe Arg Gln Met Cys Lys Arg Pro Cys Phe His Asp Asp Tyr Leu Pro 
385                 390                 395                 400 
Ala Phe Asn Arg Pro Asn Thr His Leu Val Asp Thr Gly Gly Lys Gly 
                405                 410                 415     
Val Glu Arg Ile Thr Glu Asn Gly Val Val Val Ala Gly Val Glu Tyr 
            420                 425                 430         
Glu Val Asp Cys Ile Val Tyr Ala Ser Gly Phe Glu Phe Leu Gly Thr 
        435                 440                 445             
Gly Tyr Thr Asp Arg Ala Gly Phe Asp Pro Thr Gly Arg Asp Gly Val 
    450                 455                 460                 
Lys Leu Ser Glu His Trp Ala Gln Gly Thr Arg Thr Leu His Gly Met 
465                 470                 475                 480 
His Thr Tyr Gly Phe Pro Asn Leu Phe Val Leu Gln Leu Met Gln Gly 
                485                 490                 495     
Ala Ala Leu Gly Ser Asn Ile Pro His Asn Phe Val Glu Ala Ala Arg 
            500                 505                 510         
Val Val Ala Ala Ile Val Asp His Val Leu Ser Thr Gly Thr Ser Ser 
        515                 520                 525             
Val Glu Thr Thr Lys Glu Ala Glu Gln Ala Trp Val Gln Leu Leu Leu 
    530                 535                 540                 
Asp His Gly Arg Pro Leu Gly Asn Pro Glu Cys Thr Pro Gly Tyr Tyr 
545                 550                 555                 560 
Asn Asn Glu Gly Lys Pro Ala Glu Leu Lys Asp Arg Leu Asn Val Gly 
                565                 570                 575     
Tyr Pro Ala Gly Ser Ala Ala Phe Phe Arg Met Met Asp His Trp Leu 
            580                 585                 590         
Ala Ala Gly Ser Phe Asp Gly Leu Thr Phe Arg 
        595                 600             

