                         SEQUENCE LISTING

<110>  Howell, Alexandra
       Eszterhas, Susan
       Hayden, Matthew
 
<120>  METHODS AND COMPOSITIONS FOR CRISPR/CAS9 GUIDE RNA EFFICIENCY AND
        SPECIFICITY AGAINST GENETICALLY DIVERSE HIV-1 ISOLATES

<130>  37759.0296P1

<140>  PCT/US2021/041385
<141>  2021-07-13

<150>  63/051,212
<151>  2020-07-13

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; gRNA binding site

<400>  1
ttggatggtg cttcaagtta                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct, gRNA binding site

<400>  2
ctacaaggga ctttccgctg                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; gRNA binding site

<400>  3
tctacaaggg actttccgct                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reverse primer

<400>  4
acaggccagg attaactgcg                                                   20


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; forward primer

<400>  5
ggaagggcta attcactccc aa                                                22


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hairpin adapter


<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  phosphorothioate linkage between position 29 and 30

<400>  6
cggtggaccg atgatcuatc ggtccaccgt                                        30


