                         SEQUENCE LISTING

<110>  The Rockefeller University
 
<120>  SEQUENCE SPECIFIC DEGRADATION OF SINGLE-STRANDED POLYNUCLEOTIDES 
       WITH CARD1 NUCLEASE

<130>  076091.00111

<150>  US 63/120,565
<151>  2020-12-02

<150>  US 63/054,157
<151>  2020-07-20

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  373
<212>  PRT
<213>  Treponema succinifaciens

<400>  1

Met Lys Glu Thr Ile Leu Val Asn Leu Val Ser Glu Gln Thr Ile Pro 
1               5                   10                  15      


Asn Val Gln Phe Ile Lys Trp Tyr Phe Asn Lys Lys Gln Thr Pro Met 
            20                  25                  30          


Lys Ile Leu Leu Val Ser Thr Lys Glu Met Glu Gln Lys Glu Lys Ser 
        35                  40                  45              


Leu Phe Ile Lys Asn Ala Leu His Phe Ser Asp Ser Phe Val Glu Trp 
    50                  55                  60                  


Glu Thr Ile His Thr Asp Gly Asn Asp Ile Ser Lys Thr Glu Asn Ile 
65                  70                  75                  80  


Leu Thr Asp Tyr Phe Arg Asp Asn Glu Tyr Lys Asn Ile Ile Val Asn 
                85                  90                  95      


Ile Thr Gly Gly Thr Lys Ile Met Ser Leu Ala Ala Phe Asp Phe Phe 
            100                 105                 110         


Asn Asn Lys Pro Asn Thr Glu Ile Phe Tyr Gln Pro Ile Gly Lys Glu 
        115                 120                 125             


Leu Gln Glu Leu Tyr Pro Asn Lys Gln Lys Tyr Asp Met Phe Glu Val 
    130                 135                 140                 


Leu Ser Leu Lys Glu Tyr Leu Asp Ala His Gly Ile Ser Tyr Lys Tyr 
145                 150                 155                 160 


Asp Asn Glu Cys Val Lys Asp Trp Asn Tyr Asn Lys Thr Val Tyr Asp 
                165                 170                 175     


Leu Cys Val Ala Asp Asn Arg Glu Leu Ile Lys Gly Met Ile Ala Leu 
            180                 185                 190         


Gln Asn Asn Ser Tyr Phe Asn Asn Val Tyr Lys Arg Lys Asp Phe Leu 
        195                 200                 205             


Asp Phe Thr Gln Ile Glu Glu Glu Lys Phe Ile Ala Ile Asn His Pro 
    210                 215                 220                 


Ala Ala Thr Lys Glu Asn Met Ile Lys Ile Leu Gln Ile Phe Gly Phe 
225                 230                 235                 240 


Asp Val Ser Arg Ile Glu His Lys His Ile Arg Tyr Ile Thr Gly Gly 
                245                 250                 255     


Trp Phe Glu Glu Tyr Val Tyr Gln Lys Ile Cys Asn Glu Tyr His Asn 
            260                 265                 270         


Val Asp Glu Lys Asn Val Ala Leu Asn Val Thr Ile Gln Lys Gly Asn 
        275                 280                 285             


Asp Lys Asn Glu Leu Asp Val Ile Tyr Leu Asp Lys Asp Asn Lys Leu 
    290                 295                 300                 


His Val Ile Glu Cys Lys Ser Phe Val Asp Gly Asn Glu Gly Asn Arg 
305                 310                 315                 320 


Val Leu Asn Asp Ala Leu Tyr Lys Leu Gln Ala Ile Ile Lys Ser Lys 
                325                 330                 335     


Phe Gly Leu Tyr Val Lys Gln His Leu Tyr Thr Lys Ser Ile Ile Glu 
            340                 345                 350         


Lys Glu Thr Pro Leu Asn Arg Ala Lys Glu Phe Gly Ile Asp Ile Lys 
        355                 360                 365             


Asp Gly Thr Gln Leu 
    370             


<210>  2
<211>  1122
<212>  DNA
<213>  Treponema succinifaciens

<400>  2
atgaaagaga ctattttggt taacttggtt agtgagcaaa caattcctaa tgtacaattt       60

atcaagtggt actttaataa aaagcaaaca ccaatgaaga tattgttagt gagtactaag      120

gagatggaac agaaagaaaa atcattgttc atcaagaacg ctttacactt ttcagattca      180

ttcgtggagt gggagacaat tcacactgac ggaaacgaca tatcaaagac agaaaatatt      240

ttgacagact atttcagaga taatgaatac aaaaatataa tagttaatat tacgggcggt      300

acaaagatca tgtctttagc agcatttgat ttctttaata acaaacctaa cacagagata      360

ttctaccaac ctataggtaa agaattgcag gagttatacc caaataagca aaagtacgac      420

atgttcgaag tgttatcttt aaaggaatat ttggatgcgc atggtattag ttataaatac      480

gataacgagt gtgtcaaaga ctggaactat aataagacgg tatatgattt gtgcgttgcg      540

gacaatcgtg aattaataaa gggcatgatc gctttgcaga acaactcata tttcaacaat      600

gtatataagc gaaaagattt tttagacttt actcagattg aggaggagaa gttcattgct      660

atcaatcatc cagcggctac aaaggagaat atgattaaga tcttacaaat atttggattt      720

gatgttagtc gaattgagca caagcacatc cgttatataa ctggcggttg gttcgaggag      780

tatgtatatc agaaaatttg caatgaatac cataacgtcg atgaaaagaa cgtagcgtta      840

aacgttacaa ttcagaaggg aaatgacaaa aacgagttgg atgttatcta cttggacaaa      900

gacaataagt tacacgtgat agaatgtaag agtttcgtcg atggaaacga aggcaacaga      960

gtattgaacg acgcgttata taagttacaa gcgatcatca agagtaagtt cggattatat     1020

gtaaagcaac acttgtatac gaaaagtatt atagaaaaag aaactccatt gaacagagct     1080

aaagagtttg gaattgacat taaagacggt acacaattat ga                        1122


<210>  3
<211>  757
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Representative Cas10 amino acid sequence

<400>  3

Met Asn Lys Lys Asn Ile Leu Met Tyr Gly Ser Leu Leu His Asp Ile 
1               5                   10                  15      


Gly Lys Ile Ile Tyr Arg Ser Gly Asp His Thr Phe Ser Arg Gly Thr 
            20                  25                  30          


His Ser Lys Leu Gly His Gln Phe Leu Ser Gln Phe Ser Glu Phe Lys 
        35                  40                  45              


Asp Asn Glu Val Leu Asp Asn Val Ala Tyr His His Tyr Lys Glu Leu 
    50                  55                  60                  


Ala Lys Ala Asn Leu Asp Asn Asp Asn Thr Ala Tyr Ile Thr Tyr Ile 
65                  70                  75                  80  


Ala Asp Asn Ile Ala Ser Gly Ile Asp Arg Arg Asp Ile Ile Glu Glu 
                85                  90                  95      


Gly Asp Glu Glu Tyr Glu Lys Gln Leu Phe Asn Phe Asp Lys Tyr Thr 
            100                 105                 110         


Pro Leu Tyr Ser Val Phe Asn Ile Val Asn Ser Glu Lys Leu Lys Gln 
        115                 120                 125             


Thr Asn Gly Lys Phe Lys Phe Ser Asn Glu Ser Asn Ile Glu Tyr Pro 
    130                 135                 140                 


Lys Thr Glu Asn Ile Gln Tyr Ser Ser Gly Asn Tyr Thr Thr Leu Met 
145                 150                 155                 160 


Lys Asp Met Ser His Asp Leu Glu His Lys Leu Ser Ile Lys Glu Gly 
                165                 170                 175     


Thr Phe Pro Ser Leu Leu Gln Trp Thr Glu Ser Leu Trp Gln Tyr Val 
            180                 185                 190         


Pro Ser Ser Thr Asn Lys Asn Gln Leu Ile Asp Ile Ser Leu Tyr Asp 
        195                 200                 205             


His Ser Arg Ile Thr Cys Ala Ile Ala Ser Cys Ile Phe Asp Tyr Leu 
    210                 215                 220                 


Asn Glu Asn Asn Ile His Asn Tyr Lys Asp Glu Leu Phe Ser Lys Tyr 
225                 230                 235                 240 


Glu Asn Thr Lys Ser Phe Tyr Gln Lys Glu Ala Phe Leu Leu Leu Ser 
                245                 250                 255     


Met Asp Met Ser Gly Ile Gln Asp Phe Ile Tyr Asn Ile Ser Gly Ser 
            260                 265                 270         


Lys Ala Leu Lys Ser Leu Arg Ser Arg Ser Phe Tyr Leu Glu Leu Met 
        275                 280                 285             


Leu Glu Val Ile Val Asp Gln Leu Leu Glu Arg Leu Glu Leu Ala Arg 
    290                 295                 300                 


Ala Asn Leu Leu Tyr Thr Gly Gly Gly His Ala Tyr Leu Leu Val Ser 
305                 310                 315                 320 


Asn Thr Asp Lys Val Lys Lys Lys Ile Thr Gln Phe Asn Asn Glu Leu 
                325                 330                 335     


Lys Lys Trp Phe Met Ser Glu Phe Thr Thr Asp Leu Ser Leu Ser Met 
            340                 345                 350         


Ala Phe Glu Lys Cys Ser Gly Asp Asp Leu Met Asn Thr Ser Gly Asn 
        355                 360                 365             


Tyr Arg Thr Ile Trp Arg Asn Val Ser Ser Lys Leu Ser Asp Ile Lys 
    370                 375                 380                 


Ala His Lys Tyr Ser Ala Glu Asp Ile Leu Lys Leu Asn His Phe His 
385                 390                 395                 400 


Ser Tyr Gly Asp Arg Glu Cys Lys Glu Cys Leu Arg Ser Asp Ile Asp 
                405                 410                 415     


Ile Asn Asp Asp Gly Leu Cys Ser Ile Cys Glu Gly Ile Ile Asn Ile 
            420                 425                 430         


Ser Asn Asp Leu Arg Asp Lys Ser Phe Phe Val Leu Ser Glu Thr Gly 
        435                 440                 445             


Lys Leu Lys Met Pro Phe Asn Lys Phe Ile Ser Val Ile Asp Tyr Glu 
    450                 455                 460                 


Glu Ala Glu Met Leu Val Gln Asn Asn Asn Gln Val Arg Ile Tyr Ser 
465                 470                 475                 480 


Lys Asn Lys Pro Tyr Ile Gly Ile Gly Ile Ser Thr Asn Leu Trp Met 
                485                 490                 495     


Cys Asp Tyr Asp Tyr Ala Ser Gln Asn Gln Asp Met Arg Glu Lys Gly 
            500                 505                 510         


Ile Gly Ser Tyr Val Asp Arg Glu Glu Gly Val Lys Arg Leu Gly Val 
        515                 520                 525             


Val Arg Ala Asp Ile Asp Asn Leu Gly Ala Thr Phe Ile Ser Gly Ile 
    530                 535                 540                 


Pro Glu Lys Tyr Asn Ser Ile Ser Arg Thr Ala Thr Leu Ser Arg Gln 
545                 550                 555                 560 


Leu Ser Leu Phe Phe Lys Tyr Glu Leu Asn His Leu Leu Glu Asn Tyr 
                565                 570                 575     


Gln Ile Thr Ala Ile Tyr Ser Gly Gly Asp Asp Leu Phe Leu Ile Gly 
            580                 585                 590         


Ala Trp Asp Asp Ile Ile Glu Ala Ser Ile Tyr Ile Asn Asp Lys Phe 
        595                 600                 605             


Lys Glu Phe Thr Leu Asp Lys Leu Thr Leu Ser Ala Gly Val Gly Met 
    610                 615                 620                 


Phe Ser Gly Lys Tyr Pro Val Ser Lys Met Ala Phe Glu Thr Gly Arg 
625                 630                 635                 640 


Leu Glu Glu Ala Ala Lys Thr Gly Glu Lys Asn Gln Ile Ser Leu Trp 
                645                 650                 655     


Leu Gln Glu Lys Val Tyr Asn Trp Asp Glu Phe Lys Lys Asn Ile Leu 
            660                 665                 670         


Glu Glu Lys Leu Leu Val Leu Gln Gln Gly Phe Ser Gln Thr Asp Glu 
        675                 680                 685             


His Gly Lys Ala Phe Ile Tyr Lys Met Leu Ala Leu Leu Arg Asn Asn 
    690                 695                 700                 


Glu Ala Ile Asn Ile Ala Arg Leu Ala Tyr Leu Leu Ala Arg Ser Lys 
705                 710                 715                 720 


Met Asn Glu Asp Phe Thr Ser Lys Ile Phe Asn Trp Ala Gln Asn Asp 
                725                 730                 735     


Lys Asp Lys Asn Gln Leu Ile Thr Ala Leu Glu Tyr Tyr Ile Tyr Gln 
            740                 745                 750         


Ile Arg Glu Ala Asp 
        755         


<210>  4
<211>  2274
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Representative Cas10 nucleotide coding sequence

<400>  4
atgaataaaa aaaatatatt aatgtatggc tctttattac atgatatagg gaaaattata       60

tatcgaagtg gtgatcatac attttcaaga ggtacgcatt caaaattagg tcatcaattt      120

ttgtcccaat tttcagaatt taaagacaac gaagtgcttg ataacgttgc ttatcatcat      180

tacaaagaac tcgcaaaagc taatttagat aatgataata cagcttatat tacctatatt      240

gcggataata ttgcgagtgg tattgataga agagatatta tagaagaagg cgatgaagaa      300

tacgaaaaac aactatttaa ttttgataaa tatacaccgc tatatagtgt gtttaatatt      360

gtgaattctg aaaaattgaa acaaacaaac gggaagttta aattttctaa tgaaagtaat      420

attgaatatc ctaaaactga aaacattcaa tattcaagtg gaaattatac aacattaatg      480

aaagatatga gtcatgattt agagcacaaa ttaagtatta aagaaggtac atttccttca      540

ttattacaat ggacggaaag tctatggcaa tatgtaccta gttcgacaaa taaaaaccaa      600

ttaattgata tttctcttta tgatcatagt cgtattacat gtgccatcgc cagttgtata      660

tttgattatt taaatgaaaa taacatacat aattacaaag atgaattgtt ctcaaagtat      720

gaaaatacca aatcatttta tcaaaaagaa gcttttttac tacttagtat ggatatgagt      780

ggtattcaag attttattta caatataagc ggttctaaag cattaaagag tctaagatct      840

cgtagttttt atttagaact catgcttgaa gtaatcgttg atcaattatt agaaagatta      900

gaattagcac gagcaaatct tttgtataca ggtggtggcc atgcttattt attagtgtct      960

aatactgata aagtgaagaa aaaaataact caatttaata atgaattaaa aaaatggttt     1020

atgtcagaat ttactacaga tctttcatta tcaatggctt ttgaaaaatg tagtggcgat     1080

gatttaatga atacaagtgg taattataga actatttggc gtaatgttag cagcaaactt     1140

tctgatatta aagcgcataa atattccgcg gaagatatat taaaattaaa tcattttcat     1200

tcgtatggag atcgggaatg taaagaatgt ttaagaagtg acatagatat taatgatgat     1260

ggactatgta gtatatgtga aggaattatt aatatatcaa atgatttaag agataaatca     1320

ttctttgtac tgtcagaaac tggaaaatta aaaatgccat tcaataaatt tatatcggtt     1380

attgattatg aagaggcaga aatgttagta caaaataata atcaagttcg tatttacagt     1440

aaaaataaac catatatagg cataggaata tcaacaaatt tatggatgtg tgattacgac     1500

tatgctagtc aaaatcaaga tatgagagaa aaaggtattg gaagttatgt agatagagaa     1560

gaaggggtta agcgtttagg cgtggtacgt gccgatatag ataatctcgg tgctacattt     1620

atatctggaa ttccagaaaa atataattca atttcaagaa cagctacatt gtctcgtcaa     1680

ttatcattat tttttaaata cgaattaaat catttattag aaaattatca aattactgct     1740

atatattcag gcggtgacga tttattttta atcggtgcat gggatgacat tatagaagca     1800

agcatttata taaatgacaa atttaaagag tttactcttg ataaactaac attgtctgcc     1860

ggggttggaa tgtttagtgg taagtaccca gtttctaaaa tggcttttga gacaggacga     1920

cttgaagaag cggctaagac tggtgaaaaa aatcagatat ctctttggtt acaagaaaaa     1980

gtatataact gggatgagtt taaaaagaat atcttagaag aaaaacttct cgttttacaa     2040

caggggtttt ctcaaacaga tgaacacggg aaagccttca tttataaaat gctcgcttta     2100

ctgagaaata atgaagctat taatattgct cgtttagctt acttattagc aagaagcaag     2160

atgaatgagg attttacgtc taaaattttt aattgggctc aaaacgacaa agataaaaat     2220

caattaatta cagcgttaga gtattatatt tatcaaataa gggaggctga ttga           2274


<210>  5
<211>  232
<212>  PRT
<213>  Listeriaphage (PhiLS46)

<400>  5

Met Ile Tyr Tyr Ile Lys Asp Leu Lys Val Lys Gly Lys Ile Phe Glu 
1               5                   10                  15      


Asn Leu Met Asn Lys Glu Ala Val Glu Gly Leu Ile Thr Phe Leu Lys 
            20                  25                  30          


Lys Ala Glu Phe Glu Ile Tyr Ser Arg Glu Asn Tyr Ser Lys Tyr Asn 
        35                  40                  45              


Lys Trp Phe Glu Met Trp Lys Ser Pro Thr Ser Ser Leu Val Phe Trp 
    50                  55                  60                  


Lys Asn Tyr Ser Phe Arg Cys His Leu Leu Phe Val Ile Glu Lys Asp 
65                  70                  75                  80  


Gly Glu Cys Leu Gly Ile Pro Ala Ser Val Phe Glu Ser Val Leu Gln 
                85                  90                  95      


Ile Tyr Leu Ala Asp Pro Phe Ala Pro Asp Thr Lys Glu Leu Phe Val 
            100                 105                 110         


Glu Val Cys Asn Leu Tyr Glu Cys Leu Ala Asp Val Thr Val Val Glu 
        115                 120                 125             


His Phe Glu Ala Glu Glu Ser Ala Trp His Lys Leu Thr His Asn Glu 
    130                 135                 140                 


Thr Glu Val Ser Lys Arg Val Tyr Ser Lys Asp Asp Asp Glu Leu Leu 
145                 150                 155                 160 


Lys Tyr Ile Pro Glu Phe Leu Asp Thr Ile Ala Thr Asn Lys Lys Ser 
                165                 170                 175     


Gln Lys Tyr Asn Gln Ile Gln Gly Lys Ile Gln Glu Ile Asn Lys Glu 
            180                 185                 190         


Ile Ala Thr Leu Tyr Glu Ser Ser Glu Asp Tyr Ile Phe Thr Glu Tyr 
        195                 200                 205             


Val Ser Asn Leu Tyr Arg Glu Ser Ala Lys Leu Glu Gln His Ser Lys 
    210                 215                 220                 


Gln Ile Leu Lys Glu Glu Leu Asn 
225                 230         


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  6
catattgcct gatgaagtga atag                                              24


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  7
ctattcactt catcaggcaa tatg                                              24


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  8
gaacagtgtc taacaactgc aattcactaa atgctgtaa                              39


<210>  9
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  9
gatcttacag catttagtga attgcagttg ttagacact                              39


<210>  10
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  10
atgataaata aaattacagt agagttagac ttgc                                   34


<210>  11
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  11
tatagcacct cattatttaa ctcttgaaaa c                                      31


<210>  12
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  12
caagagttaa ataatgaggt gctataatga aagagactat tttggttaac ttgg             54


<210>  13
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  13
ctaactctac tgtaatttta tttatcatat gtatatctcc ttctcataat tgtgtaccgt       60

ctttaatgtc                                                              70


<210>  14
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  14
gttacacgtg atagcatgtg ctagtttcgt cgatggaaac g                           41


<210>  15
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  15
gacgaaacta gcacatgcta tcacgtgtaa cttattgtct ttgtc                       45


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  16
gatccggctg ctaacaaagc                                                   20


<210>  17
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  17
atgtatatct ccttcttaaa gttaaacaaa attatttcta g                           41


<210>  18
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  18
gtttaacttt aagaaggaga tatacatatg aaagagacta ttttggttaa cttgg            55


<210>  19
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  19
cgggctttgt tagcagccgg atcttagtga tggtgatggt gatgtcctg                   49


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  20
caacaaaagt atttaaactt tcttttaaga ctc                                    33


<210>  21
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  21
gaaagtttaa atacttttgt tgccaatgtc attcacctac ttaattttaa gatttg           56


<210>  22
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  22
aacaattcct aatgtacaat ttatcaagtg g                                      31


<210>  23
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  23
gataaattgt acattaggaa ttgttgcctc actaaccaag ttaaccaaaa tagtc            55


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  24
gagcaaacaa ttcctaatgt acaatttatc                                        30


<210>  25
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  25
gtacattagg aattgtttgc tctgcaacca agttaaccaa aatagtctct ttc              53


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  26
atggaacaga aagaaaaatc attgttc                                           27


<210>  27
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  27
caatgatttt tctttctgtt ccattgcctt agtactcact aacaatatct tcattgg          57


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  28
gaacagaaag aaaaatcatt gttcatcaag                                        30


<210>  29
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  29
gaacaatgat ttttctttct gttctgcctc cttagtactc actaacaata tcttcattgg       60


<210>  30
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  30
aggtaaagaa ttgcaggagt tatacc                                            26


<210>  31
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  31
gtataactcc tgcaattctt tacctgcagg ttggtagaat atctctgtgt tagg             54


<210>  32
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  32
gtaaagcaac acttgtatac gaaaag                                            26


<210>  33
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  33
cttttcgtat acaagtgttg ctttactgct aatccgaact tactcttgat gatcg            55


<210>  34
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  34
atgtaagagt ttcgtcgatg gaaacg                                            26


<210>  35
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  35
ccatcgacga aactcttaca tgctatcacg tgtaacttat tgtctttgtc c                51


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  36
aatgtaagag tttcgtcgat gg                                                22


<210>  37
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  37
ccatcgacga aactcttaca ttgtatcacg tgtaacttat tgtctttgtc c                51


<210>  38
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  38
caacctatag gtaaagaatt gcagg                                             25


<210>  39
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  39
caattcttta cctataggtt gtgcgaatat ctctgtgtta ggtttgttat taaagaaatc       60


<210>  40
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  40
gttatctact tggacaaaga caataagtta c                                      31


<210>  41
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  41
gtctttgtcc aagtagataa ctgccaactc gtttttgtca tttccc                      46


<210>  42
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
gttcagttac attagataat gcgctaggtg                                        30


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
cgataaatgc tgccaccccg                                                   20


<210>  44
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
ctgtgacttt accaataact ggcacatg                                          28


<210>  45
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
ctgttcatgg tcacctttca cgtcttg                                           27


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
cgatactata tatgctgaag gacaacgacg                                        30


<210>  47
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
cttgttgttt tttgatctat tgaaattgct ggc                                    33


<210>  48
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
cgagtgtttc ttgtattgaa aaggggc                                           27


<210>  49
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
gtggtatttc aaacaatcgc gaccc                                             25


<210>  50
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  50
ggttatacta aaagtcgttt gttgg                                             25


<210>  51
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  51
ccaacaaacg acttttagta taacc                                             25


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  52
gtcaatgacc ataacgcaga ag                                                22


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  53
caatcggtgt tactaaatcc atg                                               23


<210>  54
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  54
ccgattaaaa ataaagctgc accgcctgaa tatatagcag taatttg                     47


<210>  55
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo used in cloning

<400>  55
tattcaggcg gtgcagcttt atttttaatc ggtgcatggg atg                         43


