               SEQUENCE LISTING

<110> Heinrich-Pette-Institut

<120> REPLICATION-DEFICIENT ADENOVIRUS

<130> P 111587

<160> 29

<170> BiSSAP 1.3.6

<210> 1
<211> 529
<212> PRT
<213> Adenoviridae


<220> 
<223> Wild-type amino acid sequence of DBP of HAdV-5

<400> 1
Met Ala Ser Arg Glu Glu Glu Gln Arg Glu Thr Thr Pro Glu Arg Gly 
1               5                   10                  15      
Arg Gly Ala Ala Arg Arg Pro Pro Thr Met Glu Asp Val Ser Ser Pro 
            20                  25                  30          
Ser Pro Ser Pro Pro Pro Pro Arg Ala Pro Pro Lys Lys Arg Met Arg 
        35                  40                  45              
Arg Arg Ile Glu Ser Glu Asp Glu Glu Asp Ser Ser Gln Asp Ala Leu 
    50                  55                  60                  
Val Pro Arg Thr Pro Ser Pro Arg Pro Ser Thr Ser Ala Ala Asp Leu 
65                  70                  75                  80  
Ala Ile Ala Pro Lys Lys Lys Lys Lys Arg Pro Ser Pro Lys Pro Glu 
                85                  90                  95      
Arg Pro Pro Ser Pro Glu Val Ile Val Asp Ser Glu Glu Glu Arg Glu 
            100                 105                 110         
Asp Val Ala Leu Gln Met Val Gly Phe Ser Asn Pro Pro Val Leu Ile 
        115                 120                 125             
Lys His Gly Lys Gly Gly Lys Arg Thr Val Arg Arg Leu Asn Glu Asp 
    130                 135                 140                 
Asp Pro Val Ala Arg Gly Met Arg Thr Gln Glu Glu Glu Glu Glu Pro 
145                 150                 155                 160 
Ser Glu Ala Glu Ser Glu Ile Thr Val Met Asn Pro Leu Ser Val Pro 
                165                 170                 175     
Ile Val Ser Ala Trp Glu Lys Gly Met Glu Ala Ala Arg Ala Leu Met 
            180                 185                 190         
Asp Lys Tyr His Val Asp Asn Asp Leu Lys Ala Asn Phe Lys Leu Leu 
        195                 200                 205             
Pro Asp Gln Val Glu Ala Leu Ala Ala Val Cys Lys Thr Trp Leu Asn 
    210                 215                 220                 
Glu Glu His Arg Gly Leu Gln Leu Thr Phe Thr Ser Asn Lys Thr Phe 
225                 230                 235                 240 
Val Thr Met Met Gly Arg Phe Leu Gln Ala Tyr Leu Gln Ser Phe Ala 
                245                 250                 255     
Glu Val Thr Tyr Lys His His Glu Pro Thr Gly Cys Ala Leu Trp Leu 
            260                 265                 270         
His Arg Cys Ala Glu Ile Glu Gly Glu Leu Lys Cys Leu His Gly Ser 
        275                 280                 285             
Ile Met Ile Asn Lys Glu His Val Ile Glu Met Asp Val Thr Ser Glu 
    290                 295                 300                 
Asn Gly Gln Arg Ala Leu Lys Glu Gln Ser Ser Lys Ala Lys Ile Val 
305                 310                 315                 320 
Lys Asn Arg Trp Gly Arg Asn Val Val Gln Ile Ser Asn Thr Asp Ala 
                325                 330                 335     
Arg Cys Cys Val His Asp Ala Ala Cys Pro Ala Asn Gln Phe Ser Gly 
            340                 345                 350         
Lys Ser Cys Gly Met Phe Phe Ser Glu Gly Ala Lys Ala Gln Val Ala 
        355                 360                 365             
Phe Lys Gln Ile Lys Ala Phe Met Gln Ala Leu Tyr Pro Asn Ala Gln 
    370                 375                 380                 
Thr Gly His Gly His Leu Leu Met Pro Leu Arg Cys Glu Cys Asn Ser 
385                 390                 395                 400 
Lys Pro Gly His Ala Pro Phe Leu Gly Arg Gln Leu Pro Lys Leu Thr 
                405                 410                 415     
Pro Phe Ala Leu Ser Asn Ala Glu Asp Leu Asp Ala Asp Leu Ile Ser 
            420                 425                 430         
Asp Lys Ser Val Leu Ala Ser Val His His Pro Ala Leu Ile Val Phe 
        435                 440                 445             
Gln Cys Cys Asn Pro Val Tyr Arg Asn Ser Arg Ala Gln Gly Gly Gly 
    450                 455                 460                 
Pro Asn Cys Asp Phe Lys Ile Ser Ala Pro Asp Leu Leu Asn Ala Leu 
465                 470                 475                 480 
Val Met Val Arg Ser Leu Trp Ser Glu Asn Phe Thr Glu Leu Pro Arg 
                485                 490                 495     
Met Val Val Pro Glu Phe Lys Trp Ser Thr Lys His Gln Tyr Arg Asn 
            500                 505                 510         
Val Ser Leu Pro Val Ala His Ser Asp Ala Arg Gln Asn Pro Phe Asp 
        515                 520                 525             
Phe 
    

<210> 2
<211> 529
<212> PRT
<213> Adenoviridae


<220> 
<223> Mutated amino acid sequence of DBP of HAdV-5

<400> 2
Met Ala Ser Arg Glu Glu Glu Gln Arg Glu Thr Thr Pro Glu Arg Gly 
1               5                   10                  15      
Arg Gly Ala Ala Arg Arg Pro Pro Thr Met Glu Asp Val Ser Ser Pro 
            20                  25                  30          
Ser Pro Ser Pro Pro Pro Pro Arg Ala Pro Pro Lys Lys Arg Met Arg 
        35                  40                  45              
Arg Arg Ile Glu Ser Glu Asp Glu Glu Asp Ser Ser Gln Asp Ala Leu 
    50                  55                  60                  
Val Pro Arg Thr Pro Ser Pro Arg Pro Ser Thr Ser Ala Ala Asp Leu 
65                  70                  75                  80  
Ala Ile Ala Pro Lys Lys Lys Lys Lys Arg Pro Ser Pro Lys Pro Glu 
                85                  90                  95      
Arg Pro Pro Ser Pro Glu Val Ile Val Asp Ser Glu Glu Glu Arg Glu 
            100                 105                 110         
Asp Val Ala Leu Gln Met Val Gly Phe Ser Asn Pro Pro Val Leu Ile 
        115                 120                 125             
Lys His Gly Lys Gly Gly Lys Arg Thr Val Arg Arg Leu Asn Glu Asp 
    130                 135                 140                 
Asp Pro Val Ala Arg Gly Met Arg Thr Gln Glu Glu Glu Glu Glu Pro 
145                 150                 155                 160 
Ser Glu Ala Glu Ser Glu Ile Thr Val Met Asn Pro Leu Ser Val Pro 
                165                 170                 175     
Ile Val Ser Ala Trp Glu Lys Gly Met Glu Ala Ala Arg Ala Leu Met 
            180                 185                 190         
Asp Lys Tyr His Val Asp Asn Asp Leu Lys Ala Asn Phe Lys Leu Leu 
        195                 200                 205             
Pro Asp Gln Val Glu Ala Leu Ala Ala Val Cys Lys Thr Trp Leu Asn 
    210                 215                 220                 
Glu Glu His Arg Gly Leu Gln Leu Thr Phe Thr Ser Asn Lys Thr Phe 
225                 230                 235                 240 
Val Thr Met Met Gly Arg Phe Leu Gln Ala Tyr Leu Gln Ser Phe Ala 
                245                 250                 255     
Glu Val Thr Tyr Lys His His Glu Pro Thr Gly Cys Ala Leu Trp Leu 
            260                 265                 270         
His Arg Cys Ala Glu Ile Glu Gly Glu Leu Lys Cys Leu His Gly Ser 
        275                 280                 285             
Ile Met Ile Asn Lys Glu His Val Ile Glu Met Asp Val Thr Ser Glu 
    290                 295                 300                 
Asn Gly Gln Arg Ala Leu Lys Glu Gln Ser Ser Lys Ala Lys Ile Val 
305                 310                 315                 320 
Lys Asn Arg Trp Gly Arg Asn Val Val Gln Ile Ser Asn Thr Asp Ala 
                325                 330                 335     
Arg Cys Cys Val His Asp Ala Ala Cys Pro Ala Asn Gln Phe Ser Gly 
            340                 345                 350         
Lys Ala Cys Gly Met Phe Phe Ser Glu Gly Ala Lys Ala Gln Val Ala 
        355                 360                 365             
Phe Lys Gln Ile Lys Ala Phe Met Gln Ala Leu Tyr Pro Asn Ala Gln 
    370                 375                 380                 
Thr Gly His Gly His Leu Leu Met Pro Leu Arg Cys Glu Cys Asn Ser 
385                 390                 395                 400 
Lys Pro Gly His Ala Pro Phe Leu Gly Arg Gln Leu Pro Lys Leu Thr 
                405                 410                 415     
Pro Phe Ala Leu Ser Asn Ala Glu Asp Leu Asp Ala Asp Leu Ile Ser 
            420                 425                 430         
Asp Lys Ser Val Leu Ala Ser Val His His Pro Ala Leu Ile Val Phe 
        435                 440                 445             
Gln Cys Cys Asn Pro Val Tyr Arg Asn Ser Arg Ala Gln Gly Gly Gly 
    450                 455                 460                 
Pro Asn Cys Asp Phe Lys Ile Ser Ala Pro Asp Leu Leu Asn Ala Leu 
465                 470                 475                 480 
Val Met Val Arg Ser Leu Trp Ser Glu Asn Phe Thr Glu Leu Pro Arg 
                485                 490                 495     
Met Val Val Pro Glu Phe Lys Trp Ser Thr Lys His Gln Tyr Arg Asn 
            500                 505                 510         
Val Ser Leu Pro Val Ala His Ser Asp Ala Arg Gln Asn Pro Phe Asp 
        515                 520                 525             
Phe 
    

<210> 3
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 3
ccagcccgcg gccatcgacc gcggcggcgg atttggcc                             38


<210> 4
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 4
ggccaaatcc gccgccgcgg tcgatggccg cgggctgg                             38


<210> 5
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 5
ccaatcagtt ttccggcaag gcttgcggca tgttcttctc                           40


<210> 6
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 6
gagaagaaca tgccgcaagc cttgccggaa aactgattgg                           40


<210> 7
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 7
ccagcccgcg gccatcgacc gcggcggcgg atttggcc                             38


<210> 8
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 8
ggccaaatcc gccgccgcgg tcgatggccg cgggctgg                             38


<210> 9
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 9
ccaatcagtt ttccggcaag gcttgcggca tgttcttctc                           40


<210> 10
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> oligonucleotide primer

<400> 10
gagaagaaca tgccgcaagc cttgccggaa aactgattgg                           40


<210> 11
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 11
Pro Ser Pro Ser 
1               

<210> 12
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 12
Pro Ser Thr Ser 
1               

<210> 13
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 13
Val Gly Phe Ser 
1               

<210> 14
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 14
Pro Ile Val Ser 
1               

<210> 15
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 15
Ser Gly Lys Ser 
1               

<210> 16
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 16
Ser Ser Lys Ser 
1               

<210> 17
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 17
Ser Ser Arg Ser 
1               

<210> 18
<211> 4
<212> PRT
<213> Adenoviridae


<220> 
<223> binding motif

<400> 18
Ser Ala Lys Ser 
1               

<210> 19
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutated binding motif

<400> 19
Pro Ser Thr Ala 
1               

<210> 20
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutated binding motif

<400> 20
Ser Gly Lys Ala 
1               

<210> 21
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutated binding motif

<400> 21
Pro Ser Pro Ala 
1               

<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutated binding motif

<400> 22
Val Gly Phe Ala 
1               

<210> 23
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutated binding motif

<400> 23
Pro Ile Val Ala 
1               

<210> 24
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus sequence

<220> 
<221> SITE
<222> 2
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 3
<223> Xaa = Lys or Arg

<400> 24
Ser Xaa Xaa Ser 
1               

<210> 25
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus sequence

<220> 
<221> SITE
<222> 2
<223> Xaa = one or more amino acids

<220> 
<221> SITE
<222> 3
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 4
<223> Xaa = Lys or Arg

<400> 25
Ser Xaa Xaa Xaa Ser 
1               5   

<210> 26
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus seqeunce

<220> 
<221> SITE
<222> 2
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 3
<223> Xaa = one or more amino acids

<220> 
<221> SITE
<222> 4
<223> Xaa = Lys or Arg

<400> 26
Ser Xaa Xaa Xaa Ser 
1               5   

<210> 27
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus seqeunce

<220> 
<221> SITE
<222> 2
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 3
<223> Xaa = Lys or Arg

<220> 
<221> SITE
<222> 4
<223> Xaa = one or more amino acids

<400> 27
Ser Xaa Xaa Xaa Ser 
1               5   

<210> 28
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus sequence

<220> 
<221> SITE
<222> 2
<223> Xaa = one or more amino acids

<220> 
<221> SITE
<222> 3
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 4
<223> Xaa = Lys or Arg

<220> 
<221> SITE
<222> 5
<223> Xaa = one or more amino acids

<400> 28
Ser Xaa Xaa Xaa Xaa Ser 
1               5       

<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> consensus sequence

<220> 
<221> SITE
<222> 2
<223> Xaa = one or more amino acids

<220> 
<221> SITE
<222> 3
<223> Xaa = Gly, Ser or Ala

<220> 
<221> SITE
<222> 4
<223> Xaa = one or more amino acids

<220> 
<221> SITE
<222> 5
<223> Xaa = Lys or Arg

<220> 
<221> SITE
<222> 6
<223> Xaa = one or more amino acids

<400> 29
Ser Xaa Xaa Xaa Xaa Xaa Ser 
1               5           

