                         SEQUENCE LISTING

<110>  IMPERIAL COLLEGE INNOVATIONS LIMITED
       CEVA SANTE ANIMALE
 
<120>  ACTINOBACILLUS PLEUROPNEUMONIAE VACCINES

<130>  P67379WO

<140>  PCT/IB2021/000549
<141>  2021-07-30

<150>  GB 2011902.0
<151>  2020-07-30

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  1022
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  1

Met Ala Asn Ser Gln Leu Asp Arg Val Lys Gly Leu Ile Asp Ser Leu 
1               5                   10                  15      


Asn Gln His Thr Lys Ser Ala Ala Lys Ser Gly Ala Gly Ala Leu Lys 
            20                  25                  30          


Asn Gly Leu Gly Gln Val Lys Gln Ala Gly Gln Lys Leu Ile Leu Tyr 
        35                  40                  45              


Ile Pro Lys Asp Tyr Gln Ala Ser Thr Gly Ser Ser Leu Asn Asp Leu 
    50                  55                  60                  


Val Lys Ala Ala Glu Ala Leu Gly Ile Glu Val His Arg Ser Glu Lys 
65                  70                  75                  80  


Asn Gly Thr Ala Leu Ala Lys Glu Leu Phe Gly Thr Thr Glu Lys Leu 
                85                  90                  95      


Leu Gly Phe Ser Glu Arg Gly Ile Ala Leu Phe Ala Pro Gln Phe Asp 
            100                 105                 110         


Lys Leu Leu Asn Lys Asn Gln Lys Leu Ser Lys Ser Leu Gly Gly Ser 
        115                 120                 125             


Ser Glu Ala Leu Gly Gln Arg Leu Asn Lys Thr Gln Thr Ala Leu Ser 
    130                 135                 140                 


Ala Leu Gln Ser Phe Leu Gly Thr Ala Ile Ala Gly Met Asp Leu Asp 
145                 150                 155                 160 


Ser Leu Leu Arg Arg Arg Arg Asn Gly Glu Asp Val Ser Gly Ser Glu 
                165                 170                 175     


Leu Ala Lys Ala Gly Val Asp Leu Ala Ala Gln Leu Val Asp Asn Ile 
            180                 185                 190         


Ala Ser Ala Thr Gly Thr Val Asp Ala Phe Ala Glu Gln Leu Gly Lys 
        195                 200                 205             


Leu Gly Asn Ala Leu Ser Asn Thr Arg Leu Ser Gly Leu Ala Ser Lys 
    210                 215                 220                 


Leu Asn Asn Leu Pro Asp Leu Ser Leu Ala Gly Pro Gly Phe Asp Ala 
225                 230                 235                 240 


Val Ser Gly Ile Leu Ser Val Val Ser Ala Ser Phe Ile Leu Ser Asn 
                245                 250                 255     


Lys Asp Ala Asp Ala Gly Thr Lys Ala Ala Ala Gly Ile Glu Ile Ser 
            260                 265                 270         


Thr Lys Ile Leu Gly Asn Ile Gly Lys Ala Val Ser Gln Tyr Ile Ile 
        275                 280                 285             


Ala Gln Arg Val Ala Ala Gly Leu Ser Thr Thr Ala Ala Thr Gly Gly 
    290                 295                 300                 


Leu Ile Gly Ser Val Val Ala Leu Ala Ile Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 


Asn Val Ala Asp Lys Phe Glu Arg Ala Lys Gln Leu Glu Gln Tyr Ser 
                325                 330                 335     


Glu Arg Phe Lys Lys Phe Gly Tyr Glu Gly Asp Ser Leu Leu Ala Ser 
            340                 345                 350         


Phe Tyr Arg Glu Thr Gly Ala Ile Glu Ala Ala Leu Thr Thr Ile Asn 
        355                 360                 365             


Ser Val Leu Ser Ala Ala Ser Ala Gly Val Gly Ala Ala Ala Thr Gly 
    370                 375                 380                 


Ser Leu Val Gly Ala Pro Val Ala Ala Leu Val Ser Ala Ile Thr Gly 
385                 390                 395                 400 


Ile Ile Ser Gly Ile Leu Asp Ala Ser Lys Gln Ala Ile Phe Glu Arg 
                405                 410                 415     


Val Ala Thr Lys Leu Ala Asn Lys Ile Asp Glu Trp Glu Lys Lys His 
            420                 425                 430         


Gly Lys Asn Tyr Phe Glu Asn Gly Tyr Asp Ala Arg His Ser Ala Phe 
        435                 440                 445             


Leu Glu Asp Thr Phe Glu Leu Leu Ser Gln Tyr Asn Lys Glu Tyr Ser 
    450                 455                 460                 


Val Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Val Asn Ile 
465                 470                 475                 480 


Gly Glu Leu Ala Gly Ile Thr Arg Lys Gly Ala Asp Ala Lys Ser Gly 
                485                 490                 495     


Lys Ala Tyr Val Asp Phe Phe Glu Glu Gly Lys Leu Leu Glu Lys Asp 
            500                 505                 510         


Pro Asp Arg Phe Asp Lys Lys Val Phe Asp Pro Leu Glu Gly Lys Ile 
        515                 520                 525             


Asp Leu Ser Ser Ile Asn Lys Thr Thr Leu Leu Lys Phe Ile Thr Pro 
    530                 535                 540                 


Val Phe Thr Ala Gly Glu Glu Ile Arg Glu Arg Lys Gln Thr Gly Lys 
545                 550                 555                 560 


Tyr Glu Tyr Met Thr Glu Leu Phe Val Lys Gly Lys Glu Lys Trp Val 
                565                 570                 575     


Val Thr Gly Val Gln Ser His Asn Ala Ile Tyr Asp Tyr Thr Asn Leu 
            580                 585                 590         


Ile Gln Leu Ala Ile Asp Lys Lys Gly Glu Lys Arg Gln Val Thr Ile 
        595                 600                 605             


Glu Ser His Leu Gly Glu Lys Asn Asp Arg Ile Tyr Leu Ser Ser Gly 
    610                 615                 620                 


Ser Ser Ile Val Tyr Ala Gly Asn Gly His Asp Val Ala Tyr Tyr Asp 
625                 630                 635                 640 


Lys Thr Asp Thr Gly Tyr Leu Thr Phe Asp Gly Gln Ser Ala Gln Lys 
                645                 650                 655     


Ala Gly Glu Tyr Ile Val Thr Lys Glu Leu Lys Ala Asp Val Lys Val 
            660                 665                 670         


Leu Lys Glu Val Val Lys Thr Gln Asp Ile Ser Val Gly Lys Arg Ser 
        675                 680                 685             


Glu Lys Leu Glu Tyr Arg Asp Tyr Glu Leu Ser Pro Phe Glu Leu Gly 
    690                 695                 700                 


Asn Gly Ile Arg Ala Lys Asp Glu Leu His Ser Val Glu Glu Ile Ile 
705                 710                 715                 720 


Gly Ser Asn Arg Lys Asp Lys Phe Phe Gly Ser Arg Phe Thr Asp Ile 
                725                 730                 735     


Phe His Gly Ala Lys Gly Asp Asp Glu Ile Tyr Gly Asn Asp Gly His 
            740                 745                 750         


Asp Ile Leu Tyr Gly Asp Asp Gly Asn Asp Val Ile His Gly Gly Asp 
        755                 760                 765             


Gly Asn Asp His Leu Val Gly Gly Asn Gly Asn Asp Arg Leu Ile Gly 
    770                 775                 780                 


Gly Lys Gly Asn Asn Phe Leu Asn Gly Gly Asp Gly Asp Asp Glu Leu 
785                 790                 795                 800 


Gln Val Phe Glu Gly Gln Tyr Asn Val Leu Leu Gly Gly Ala Gly Asn 
                805                 810                 815     


Asp Ile Leu Tyr Gly Ser Asp Gly Thr Asn Leu Phe Asp Gly Gly Val 
            820                 825                 830         


Gly Asn Asp Lys Ile Tyr Gly Gly Leu Gly Lys Asp Ile Tyr Arg Tyr 
        835                 840                 845             


Ser Lys Glu Tyr Gly Arg His Ile Ile Ile Glu Lys Gly Gly Asp Asp 
    850                 855                 860                 


Asp Thr Leu Leu Leu Ser Asp Leu Ser Phe Lys Asp Val Gly Phe Ile 
865                 870                 875                 880 


Arg Ile Gly Asp Asp Leu Leu Val Asn Lys Arg Ile Gly Gly Thr Leu 
                885                 890                 895     


Tyr Tyr His Glu Asp Tyr Asn Gly Asn Ala Leu Thr Ile Lys Asp Trp 
            900                 905                 910         


Phe Lys Glu Gly Lys Glu Gly Gln Asn Asn Lys Ile Glu Lys Ile Val 
        915                 920                 925             


Asp Lys Asp Gly Ala Tyr Val Leu Ser Gln Tyr Leu Thr Glu Leu Thr 
    930                 935                 940                 


Ala Pro Gly Arg Gly Ile Asn Tyr Phe Asn Gly Leu Glu Glu Lys Leu 
945                 950                 955                 960 


Tyr Tyr Gly Glu Gly Tyr Asn Ala Leu Pro Gln Leu Arg Lys Asp Ile 
                965                 970                 975     


Glu Gln Ile Ile Ser Ser Thr Gly Ala Leu Thr Gly Glu His Gly Gln 
            980                 985                 990         


Val Leu Val Gly Ala Gly Gly Pro  Leu Ala Tyr Ser Asn  Ser Pro Asn 
        995                 1000                 1005             


Ser Ile  Pro Asn Ala Phe Ser  Asn Tyr Leu Thr Gln  Ser Ala 
    1010                 1015                 1020         


<210>  2
<211>  956
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  2

Met Ser Lys Ile Thr Leu Ser Ser Leu Lys Ser Ser Leu Gln Gln Gly 
1               5                   10                  15      


Leu Lys Asn Gly Lys Asn Lys Leu Asn Gln Ala Gly Thr Thr Leu Lys 
            20                  25                  30          


Asn Gly Leu Thr Gln Thr Gly His Ser Leu Gln Asn Gly Ala Lys Lys 
        35                  40                  45              


Leu Ile Leu Tyr Ile Pro Gln Gly Tyr Asp Ser Gly Gln Gly Asn Gly 
    50                  55                  60                  


Val Gln Asp Leu Val Lys Ala Ala Asn Asp Leu Gly Ile Glu Val Trp 
65                  70                  75                  80  


Arg Glu Glu Arg Ser Asn Leu Asp Ile Ala Lys Thr Ser Phe Asp Thr 
                85                  90                  95      


Thr Gln Lys Ile Leu Gly Phe Thr Asp Arg Gly Ile Val Leu Phe Ala 
            100                 105                 110         


Pro Gln Leu Asp Asn Leu Leu Lys Lys Asn Pro Lys Ile Gly Asn Thr 
        115                 120                 125             


Leu Gly Ser Ala Ser Ser Ile Ser Gln Asn Ile Gly Lys Ala Asn Thr 
    130                 135                 140                 


Val Leu Gly Gly Ile Gln Ser Ile Leu Gly Ser Val Leu Ser Gly Val 
145                 150                 155                 160 


Asn Leu Asn Glu Leu Leu Gln Asn Lys Asp Pro Asn Gln Leu Glu Leu 
                165                 170                 175     


Ala Lys Ala Gly Leu Glu Leu Thr Asn Glu Leu Val Gly Asn Ile Ala 
            180                 185                 190         


Ser Ser Val Gln Thr Val Asp Ala Phe Ala Glu Gln Ile Ser Lys Leu 
        195                 200                 205             


Gly Ser His Leu Gln Asn Val Lys Gly Leu Gly Gly Leu Ser Asn Lys 
    210                 215                 220                 


Leu Gln Asn Leu Pro Asp Leu Gly Lys Ala Ser Leu Gly Leu Asp Ile 
225                 230                 235                 240 


Ile Ser Gly Leu Leu Ser Gly Ala Ser Ala Gly Leu Ile Leu Ala Asp 
                245                 250                 255     


Lys Glu Ala Ser Thr Glu Lys Lys Ala Ala Ala Gly Val Glu Phe Ala 
            260                 265                 270         


Asn Gln Ile Ile Gly Asn Val Thr Lys Ala Val Ser Ser Tyr Ile Leu 
        275                 280                 285             


Ala Gln Arg Val Ala Ser Gly Leu Ser Ser Thr Gly Pro Val Ala Ala 
    290                 295                 300                 


Leu Ile Ala Ser Thr Val Ala Leu Ala Val Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 


Asn Val Ala Asp Lys Phe Lys Gln Ala Asp Leu Ile Lys Ser Tyr Ser 
                325                 330                 335     


Glu Arg Phe Gln Lys Leu Gly Tyr Asp Gly Asp Arg Leu Leu Ala Asp 
            340                 345                 350         


Phe His Arg Glu Thr Gly Thr Ile Asp Ala Ser Val Thr Thr Ile Asn 
        355                 360                 365             


Thr Ala Leu Ala Ala Ile Ser Gly Gly Val Gly Ala Ala Ser Ala Gly 
    370                 375                 380                 


Ser Leu Val Gly Ala Pro Val Ala Leu Leu Val Ala Gly Val Thr Gly 
385                 390                 395                 400 


Leu Ile Thr Thr Ile Leu Glu Tyr Ser Lys Gln Ala Met Phe Glu His 
                405                 410                 415     


Val Ala Asn Lys Val His Asp Arg Ile Val Glu Trp Glu Lys Lys His 
            420                 425                 430         


Asn Lys Asn Tyr Phe Glu Gln Gly Tyr Asp Ser Arg His Leu Ala Asp 
        435                 440                 445             


Leu Gln Asp Asn Met Lys Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln 
    450                 455                 460                 


Ala Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile 
465                 470                 475                 480 


Gly Asp Leu Ala Ala Ile Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly 
                485                 490                 495     


Lys Ala Tyr Val Asp Ala Phe Glu Glu Gly Gln His Gln Ser Tyr Asp 
            500                 505                 510         


Ser Ser Val Gln Leu Asp Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn 
        515                 520                 525             


Thr Asn Arg Lys Thr Gln Ser Val Leu Phe Arg Thr Pro Leu Leu Thr 
    530                 535                 540                 


Pro Gly Glu Glu Asn Arg Glu Arg Ile Gln Glu Gly Lys Asn Ser Tyr 
545                 550                 555                 560 


Ile Thr Lys Leu His Ile Gln Arg Val Asp Ser Trp Thr Val Thr Asp 
                565                 570                 575     


Gly Asp Ala Ser Ser Ser Val Asp Phe Thr Asn Val Val Gln Arg Ile 
            580                 585                 590         


Ala Val Lys Phe Asp Asp Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr 
        595                 600                 605             


Lys Ile Ile Ala Asn Leu Gly Ala Gly Asn Asp Asn Val Phe Val Gly 
    610                 615                 620                 


Ser Ser Thr Thr Val Ile Asp Gly Gly Asp Gly His Asp Arg Val His 
625                 630                 635                 640 


Tyr Ser Arg Gly Glu Tyr Gly Ala Leu Val Ile Asp Ala Thr Ala Glu 
                645                 650                 655     


Thr Glu Lys Gly Ser Tyr Ser Val Lys Arg Tyr Val Gly Asp Ser Lys 
            660                 665                 670         


Ala Leu His Glu Thr Ile Ala Thr His Gln Thr Asn Val Gly Asn Arg 
        675                 680                 685             


Glu Glu Lys Ile Glu Tyr Arg Arg Glu Asp Asp Arg Phe His Thr Gly 
    690                 695                 700                 


Tyr Thr Val Thr Asp Ser Leu Lys Ser Val Glu Glu Ile Ile Gly Ser 
705                 710                 715                 720 


Gln Phe Asn Asp Ile Phe Lys Gly Ser Gln Phe Asp Asp Val Phe His 
                725                 730                 735     


Gly Gly Asn Gly Val Asp Thr Ile Asp Gly Asn Asp Gly Asp Asp His 
            740                 745                 750         


Leu Phe Gly Gly Ala Gly Asp Asp Val Ile Asp Gly Gly Asn Gly Asn 
        755                 760                 765             


Asn Phe Leu Val Gly Gly Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys 
    770                 775                 780                 


Asp Asn Asp Ile Tyr Val His Lys Thr Gly Asp Gly Asn Asp Ser Ile 
785                 790                 795                 800 


Thr Asp Ser Gly Gly Gln Asp Lys Leu Ala Phe Ser Asp Val Asn Leu 
                805                 810                 815     


Lys Asp Leu Thr Phe Lys Lys Val Asp Ser Ser Leu Glu Ile Ile Asn 
            820                 825                 830         


Gln Lys Gly Glu Lys Val Arg Ile Gly Asn Trp Phe Leu Glu Asp Asp 
        835                 840                 845             


Leu Ala Ser Thr Val Ala Asn Tyr Lys Ala Thr Asn Asp Arg Lys Ile 
    850                 855                 860                 


Glu Glu Ile Ile Gly Lys Gly Gly Glu Arg Ile Thr Ser Glu Gln Val 
865                 870                 875                 880 


Asp Lys Leu Ile Lys Glu Gly Asn Asn Gln Ile Ser Ala Glu Ala Leu 
                885                 890                 895     


Ser Lys Val Val Asn Asp Tyr Asn Thr Ser Lys Asp Arg Gln Asn Val 
            900                 905                 910         


Ser Asn Ser Leu Ala Lys Leu Ile Ser Ser Val Gly Ser Phe Thr Ser 
        915                 920                 925             


Ser Ser Asp Phe Arg Asn Asn Leu Gly Thr Tyr Val Pro Ser Ser Ile 
    930                 935                 940                 


Asp Val Ser Asn Asn Ile Gln Leu Ala Arg Ala Ala 
945                 950                 955     


<210>  3
<211>  1052
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  3

Met Ser Thr Trp Ser Ser Met Leu Ala Asp Leu Lys Lys Arg Ala Glu 
1               5                   10                  15      


Glu Ala Lys Arg Gln Ala Lys Lys Gly Tyr Asp Val Thr Lys Asn Gly 
            20                  25                  30          


Leu Gln Tyr Gly Val Ser Gln Ala Lys Leu Gln Ala Leu Ala Ala Gly 
        35                  40                  45              


Lys Ala Val Gln Lys Tyr Gly Asn Lys Leu Val Leu Val Ile Pro Lys 
    50                  55                  60                  


Glu Tyr Asp Gly Ser Val Gly Asn Gly Phe Phe Asp Leu Val Lys Ala 
65                  70                  75                  80  


Ala Glu Glu Leu Gly Ile Gln Val Lys Tyr Val Asn Arg Asn Glu Leu 
                85                  90                  95      


Glu Val Ala His Lys Ser Leu Gly Thr Ala Asp Gln Phe Leu Gly Leu 
            100                 105                 110         


Thr Glu Arg Gly Leu Thr Leu Phe Ala Pro Gln Leu Asp Gln Phe Leu 
        115                 120                 125             


Gln Lys His Ser Lys Ile Ser Asn Val Val Gly Ser Ser Thr Gly Asp 
    130                 135                 140                 


Ala Val Ser Lys Leu Ala Lys Ser Gln Thr Ile Ile Ser Gly Ile Gln 
145                 150                 155                 160 


Ser Val Leu Gly Thr Val Leu Ala Gly Ile Asn Leu Asn Glu Ala Ile 
                165                 170                 175     


Ile Ser Gly Gly Ser Glu Leu Glu Leu Ala Glu Ala Gly Val Ser Leu 
            180                 185                 190         


Ala Ser Glu Leu Val Ser Asn Ile Ala Lys Gly Thr Thr Thr Ile Asp 
        195                 200                 205             


Ala Phe Thr Thr Gln Ile Gln Asn Phe Gly Lys Leu Val Glu Asn Ala 
    210                 215                 220                 


Lys Gly Leu Gly Gly Val Gly Arg Gln Leu Gln Asn Ile Ser Gly Ser 
225                 230                 235                 240 


Ala Leu Ser Lys Thr Gly Leu Gly Leu Asp Ile Ile Ser Ser Leu Leu 
                245                 250                 255     


Ser Gly Val Thr Ala Ser Phe Ala Leu Ala Asn Lys Asn Ala Ser Thr 
            260                 265                 270         


Ser Thr Lys Val Ala Ala Gly Phe Glu Leu Ser Asn Gln Val Ile Gly 
        275                 280                 285             


Gly Ile Thr Lys Ala Val Ser Ser Tyr Ile Leu Ala Gln Arg Leu Ala 
    290                 295                 300                 


Ala Gly Leu Ser Thr Thr Gly Pro Ala Ala Ala Leu Ile Ala Ser Ser 
305                 310                 315                 320 


Ile Ser Leu Ala Ile Ser Pro Leu Ala Phe Leu Arg Val Ala Asp Asn 
                325                 330                 335     


Phe Asn Arg Ser Lys Glu Ile Gly Glu Phe Ala Glu Arg Phe Lys Lys 
            340                 345                 350         


Leu Gly Tyr Asp Gly Asp Lys Leu Leu Ser Glu Phe Tyr His Glu Ala 
        355                 360                 365             


Gly Thr Ile Asp Ala Ser Ile Thr Thr Ile Ser Thr Ala Leu Ser Ala 
    370                 375                 380                 


Ile Ala Ala Gly Thr Ala Ala Ala Ser Ala Gly Ala Leu Val Gly Ala 
385                 390                 395                 400 


Pro Ile Thr Leu Leu Val Thr Gly Ile Thr Gly Leu Ile Ser Gly Ile 
                405                 410                 415     


Leu Glu Phe Ser Lys Gln Pro Met Leu Asp His Val Ala Ser Lys Ile 
            420                 425                 430         


Gly Asn Lys Ile Asp Glu Trp Glu Lys Lys Tyr Gly Lys Asn Tyr Phe 
        435                 440                 445             


Glu Asn Gly Tyr Asp Ala Arg His Lys Ala Phe Leu Glu Asp Ser Phe 
    450                 455                 460                 


Ser Leu Leu Ser Ser Phe Asn Lys Gln Tyr Glu Thr Glu Arg Ala Val 
465                 470                 475                 480 


Leu Ile Thr Gln Gln Arg Trp Asp Glu Tyr Ile Gly Glu Leu Ala Gly 
                485                 490                 495     


Ile Thr Gly Lys Gly Asp Lys Leu Ser Ser Gly Lys Ala Tyr Val Asp 
            500                 505                 510         


Tyr Phe Gln Glu Gly Lys Leu Leu Glu Lys Lys Pro Asp Asp Phe Ser 
        515                 520                 525             


Lys Val Val Phe Asp Pro Thr Lys Gly Glu Ile Asp Ile Ser Asn Ser 
    530                 535                 540                 


Gln Thr Ser Thr Leu Leu Lys Phe Val Thr Pro Leu Leu Thr Pro Gly 
545                 550                 555                 560 


Thr Glu Ser Arg Glu Arg Thr Gln Thr Gly Lys Tyr Glu Tyr Ile Thr 
                565                 570                 575     


Lys Leu Val Val Lys Gly Lys Asp Lys Trp Val Val Asn Gly Val Lys 
            580                 585                 590         


Asp Lys Gly Ala Val Tyr Asp Tyr Thr Asn Leu Ile Gln His Ala His 
        595                 600                 605             


Ile Ser Ser Ser Val Ala Arg Gly Glu Glu Tyr Arg Glu Val Arg Leu 
    610                 615                 620                 


Val Ser His Leu Gly Asn Gly Asn Asp Lys Val Phe Leu Ala Ala Gly 
625                 630                 635                 640 


Ser Ala Glu Ile His Ala Gly Glu Gly His Asp Val Val Tyr Tyr Asp 
                645                 650                 655     


Lys Thr Asp Thr Gly Leu Leu Val Ile Asp Gly Thr Lys Ala Thr Glu 
            660                 665                 670         


Gln Gly Arg Tyr Ser Val Thr Arg Glu Leu Ser Gly Ala Thr Lys Ile 
        675                 680                 685             


Leu Arg Glu Val Ile Lys Asn Gln Lys Ser Ala Val Gly Lys Arg Glu 
    690                 695                 700                 


Glu Thr Leu Glu Tyr Arg Asp Tyr Glu Leu Thr Gln Ser Gly Asn Ser 
705                 710                 715                 720 


Asn Leu Lys Ala His Asp Glu Leu His Ser Val Glu Glu Ile Ile Gly 
                725                 730                 735     


Ser Asn Gln Arg Asp Glu Phe Lys Gly Ser Lys Phe Arg Asp Ile Phe 
            740                 745                 750         


His Gly Ala Asp Gly Asp Asp Leu Leu Asn Gly Asn Asp Gly Asp Asp 
        755                 760                 765             


Ile Leu Tyr Gly Asp Lys Gly Asn Asp Glu Leu Arg Gly Asp Asn Gly 
    770                 775                 780                 


Asn Asp Gln Leu Tyr Gly Gly Glu Gly Asn Asp Lys Leu Leu Gly Gly 
785                 790                 795                 800 


Asn Gly Asn Asn Tyr Leu Ser Gly Gly Asp Gly Asn Asp Glu Leu Gln 
                805                 810                 815     


Val Leu Gly Asn Gly Phe Asn Val Leu Arg Gly Gly Lys Gly Asp Asp 
            820                 825                 830         


Lys Leu Tyr Gly Ser Ser Gly Ser Asp Leu Leu Asp Gly Gly Glu Gly 
        835                 840                 845             


Asn Asp Tyr Leu Glu Gly Gly Asp Gly Ser Asp Phe Tyr Val Tyr Arg 
    850                 855                 860                 


Ser Thr Ser Gly Asn His Thr Ile Tyr Asp Gln Gly Lys Ser Ser Asp 
865                 870                 875                 880 


Leu Asp Lys Leu Tyr Leu Ser Asp Phe Ser Phe Asp Arg Leu Leu Val 
                885                 890                 895     


Glu Lys Val Asp Asp Asn Leu Val Leu Arg Ser Asn Glu Ser Ser His 
            900                 905                 910         


Asn Asn Gly Val Leu Thr Ile Lys Asp Trp Phe Lys Glu Gly Asn Lys 
        915                 920                 925             


Tyr Asn His Lys Ile Glu Gln Ile Val Asp Lys Asn Gly Arg Lys Leu 
    930                 935                 940                 


Thr Ala Glu Asn Leu Gly Thr Tyr Phe Lys Asn Ala Pro Lys Ala Asp 
945                 950                 955                 960 


Asn Leu Leu Asn Tyr Ala Thr Lys Glu Asp Gln Asn Glu Ser Asn Leu 
                965                 970                 975     


Ser Ser Leu Lys Thr Glu Leu Ser Lys Ile Ile Thr Asn Ala Gly Asn 
            980                 985                 990         


Phe Gly Val Ala Lys Gln Gly Asn  Thr Gly Ile Asn Thr  Ala Ala Leu 
        995                 1000                 1005             


Asn Asn  Glu Val Asn Lys Ile  Ile Ser Ser Ala Asn  Thr Phe Ala 
    1010                 1015                 1020             


Thr Ser  Gln Leu Gly Gly Ser  Gly Met Gly Thr Leu  Pro Ser Thr 
    1025                 1030                 1035             


Asn Val  Asn Ser Met Met Leu  Gly Asn Leu Ala Arg  Ala Ala 
    1040                 1045                 1050         


<210>  4
<211>  1022
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APP ApxIA K560A K686A

<400>  4

Met Ala Asn Ser Gln Leu Asp Arg Val Lys Gly Leu Ile Asp Ser Leu 
1               5                   10                  15      


Asn Gln His Thr Lys Ser Ala Ala Lys Ser Gly Ala Gly Ala Leu Lys 
            20                  25                  30          


Asn Gly Leu Gly Gln Val Lys Gln Ala Gly Gln Lys Leu Ile Leu Tyr 
        35                  40                  45              


Ile Pro Lys Asp Tyr Gln Ala Ser Thr Gly Ser Ser Leu Asn Asp Leu 
    50                  55                  60                  


Val Lys Ala Ala Glu Ala Leu Gly Ile Glu Val His Arg Ser Glu Lys 
65                  70                  75                  80  


Asn Gly Thr Ala Leu Ala Lys Glu Leu Phe Gly Thr Thr Glu Lys Leu 
                85                  90                  95      


Leu Gly Phe Ser Glu Arg Gly Ile Ala Leu Phe Ala Pro Gln Phe Asp 
            100                 105                 110         


Lys Leu Leu Asn Lys Asn Gln Lys Leu Ser Lys Ser Leu Gly Gly Ser 
        115                 120                 125             


Ser Glu Ala Leu Gly Gln Arg Leu Asn Lys Thr Gln Thr Ala Leu Ser 
    130                 135                 140                 


Ala Leu Gln Ser Phe Leu Gly Thr Ala Ile Ala Gly Met Asp Leu Asp 
145                 150                 155                 160 


Ser Leu Leu Arg Arg Arg Arg Asn Gly Glu Asp Val Ser Gly Ser Glu 
                165                 170                 175     


Leu Ala Lys Ala Gly Val Asp Leu Ala Ala Gln Leu Val Asp Asn Ile 
            180                 185                 190         


Ala Ser Ala Thr Gly Thr Val Asp Ala Phe Ala Glu Gln Leu Gly Lys 
        195                 200                 205             


Leu Gly Asn Ala Leu Ser Asn Thr Arg Leu Ser Gly Leu Ala Ser Lys 
    210                 215                 220                 


Leu Asn Asn Leu Pro Asp Leu Ser Leu Ala Gly Pro Gly Phe Asp Ala 
225                 230                 235                 240 


Val Ser Gly Ile Leu Ser Val Val Ser Ala Ser Phe Ile Leu Ser Asn 
                245                 250                 255     


Lys Asp Ala Asp Ala Gly Thr Lys Ala Ala Ala Gly Ile Glu Ile Ser 
            260                 265                 270         


Thr Lys Ile Leu Gly Asn Ile Gly Lys Ala Val Ser Gln Tyr Ile Ile 
        275                 280                 285             


Ala Gln Arg Val Ala Ala Gly Leu Ser Thr Thr Ala Ala Thr Gly Gly 
    290                 295                 300                 


Leu Ile Gly Ser Val Val Ala Leu Ala Ile Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 


Asn Val Ala Asp Lys Phe Glu Arg Ala Lys Gln Leu Glu Gln Tyr Ser 
                325                 330                 335     


Glu Arg Phe Lys Lys Phe Gly Tyr Glu Gly Asp Ser Leu Leu Ala Ser 
            340                 345                 350         


Phe Tyr Arg Glu Thr Gly Ala Ile Glu Ala Ala Leu Thr Thr Ile Asn 
        355                 360                 365             


Ser Val Leu Ser Ala Ala Ser Ala Gly Val Gly Ala Ala Ala Thr Gly 
    370                 375                 380                 


Ser Leu Val Gly Ala Pro Val Ala Ala Leu Val Ser Ala Ile Thr Gly 
385                 390                 395                 400 


Ile Ile Ser Gly Ile Leu Asp Ala Ser Lys Gln Ala Ile Phe Glu Arg 
                405                 410                 415     


Val Ala Thr Lys Leu Ala Asn Lys Ile Asp Glu Trp Glu Lys Lys His 
            420                 425                 430         


Gly Lys Asn Tyr Phe Glu Asn Gly Tyr Asp Ala Arg His Ser Ala Phe 
        435                 440                 445             


Leu Glu Asp Thr Phe Glu Leu Leu Ser Gln Tyr Asn Lys Glu Tyr Ser 
    450                 455                 460                 


Val Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Val Asn Ile 
465                 470                 475                 480 


Gly Glu Leu Ala Gly Ile Thr Arg Lys Gly Ala Asp Ala Lys Ser Gly 
                485                 490                 495     


Lys Ala Tyr Val Asp Phe Phe Glu Glu Gly Lys Leu Leu Glu Lys Asp 
            500                 505                 510         


Pro Asp Arg Phe Asp Lys Lys Val Phe Asp Pro Leu Glu Gly Lys Ile 
        515                 520                 525             


Asp Leu Ser Ser Ile Asn Lys Thr Thr Leu Leu Lys Phe Ile Thr Pro 
    530                 535                 540                 


Val Phe Thr Ala Gly Glu Glu Ile Arg Glu Arg Lys Gln Thr Gly Ala 
545                 550                 555                 560 


Tyr Glu Tyr Met Thr Glu Leu Phe Val Lys Gly Lys Glu Lys Trp Val 
                565                 570                 575     


Val Thr Gly Val Gln Ser His Asn Ala Ile Tyr Asp Tyr Thr Asn Leu 
            580                 585                 590         


Ile Gln Leu Ala Ile Asp Lys Lys Gly Glu Lys Arg Gln Val Thr Ile 
        595                 600                 605             


Glu Ser His Leu Gly Glu Lys Asn Asp Arg Ile Tyr Leu Ser Ser Gly 
    610                 615                 620                 


Ser Ser Ile Val Tyr Ala Gly Asn Gly His Asp Val Ala Tyr Tyr Asp 
625                 630                 635                 640 


Lys Thr Asp Thr Gly Tyr Leu Thr Phe Asp Gly Gln Ser Ala Gln Lys 
                645                 650                 655     


Ala Gly Glu Tyr Ile Val Thr Lys Glu Leu Lys Ala Asp Val Lys Val 
            660                 665                 670         


Leu Lys Glu Val Val Lys Thr Gln Asp Ile Ser Val Gly Ala Arg Ser 
        675                 680                 685             


Glu Lys Leu Glu Tyr Arg Asp Tyr Glu Leu Ser Pro Phe Glu Leu Gly 
    690                 695                 700                 


Asn Gly Ile Arg Ala Lys Asp Glu Leu His Ser Val Glu Glu Ile Ile 
705                 710                 715                 720 


Gly Ser Asn Arg Lys Asp Lys Phe Phe Gly Ser Arg Phe Thr Asp Ile 
                725                 730                 735     


Phe His Gly Ala Lys Gly Asp Asp Glu Ile Tyr Gly Asn Asp Gly His 
            740                 745                 750         


Asp Ile Leu Tyr Gly Asp Asp Gly Asn Asp Val Ile His Gly Gly Asp 
        755                 760                 765             


Gly Asn Asp His Leu Val Gly Gly Asn Gly Asn Asp Arg Leu Ile Gly 
    770                 775                 780                 


Gly Lys Gly Asn Asn Phe Leu Asn Gly Gly Asp Gly Asp Asp Glu Leu 
785                 790                 795                 800 


Gln Val Phe Glu Gly Gln Tyr Asn Val Leu Leu Gly Gly Ala Gly Asn 
                805                 810                 815     


Asp Ile Leu Tyr Gly Ser Asp Gly Thr Asn Leu Phe Asp Gly Gly Val 
            820                 825                 830         


Gly Asn Asp Lys Ile Tyr Gly Gly Leu Gly Lys Asp Ile Tyr Arg Tyr 
        835                 840                 845             


Ser Lys Glu Tyr Gly Arg His Ile Ile Ile Glu Lys Gly Gly Asp Asp 
    850                 855                 860                 


Asp Thr Leu Leu Leu Ser Asp Leu Ser Phe Lys Asp Val Gly Phe Ile 
865                 870                 875                 880 


Arg Ile Gly Asp Asp Leu Leu Val Asn Lys Arg Ile Gly Gly Thr Leu 
                885                 890                 895     


Tyr Tyr His Glu Asp Tyr Asn Gly Asn Ala Leu Thr Ile Lys Asp Trp 
            900                 905                 910         


Phe Lys Glu Gly Lys Glu Gly Gln Asn Asn Lys Ile Glu Lys Ile Val 
        915                 920                 925             


Asp Lys Asp Gly Ala Tyr Val Leu Ser Gln Tyr Leu Thr Glu Leu Thr 
    930                 935                 940                 


Ala Pro Gly Arg Gly Ile Asn Tyr Phe Asn Gly Leu Glu Glu Lys Leu 
945                 950                 955                 960 


Tyr Tyr Gly Glu Gly Tyr Asn Ala Leu Pro Gln Leu Arg Lys Asp Ile 
                965                 970                 975     


Glu Gln Ile Ile Ser Ser Thr Gly Ala Leu Thr Gly Glu His Gly Gln 
            980                 985                 990         


Val Leu Val Gly Ala Gly Gly Pro  Leu Ala Tyr Ser Asn  Ser Pro Asn 
        995                 1000                 1005             


Ser Ile  Pro Asn Ala Phe Ser  Asn Tyr Leu Thr Gln  Ser Ala 
    1010                 1015                 1020         


<210>  5
<211>  956
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APP ApxIIA S148G K557A N687A

<400>  5

Met Ser Lys Ile Thr Leu Ser Ser Leu Lys Ser Ser Leu Gln Gln Gly 
1               5                   10                  15      


Leu Lys Asn Gly Lys Asn Lys Leu Asn Gln Ala Gly Thr Thr Leu Lys 
            20                  25                  30          


Asn Gly Leu Thr Gln Thr Gly His Ser Leu Gln Asn Gly Ala Lys Lys 
        35                  40                  45              


Leu Ile Leu Tyr Ile Pro Gln Gly Tyr Asp Ser Gly Gln Gly Asn Gly 
    50                  55                  60                  


Val Gln Asp Leu Val Lys Ala Ala Asn Asp Leu Gly Ile Glu Val Trp 
65                  70                  75                  80  


Arg Glu Glu Arg Ser Asn Leu Asp Ile Ala Lys Thr Ser Phe Asp Thr 
                85                  90                  95      


Thr Gln Lys Ile Leu Gly Phe Thr Asp Arg Gly Ile Val Leu Phe Ala 
            100                 105                 110         


Pro Gln Leu Asp Asn Leu Leu Lys Lys Asn Pro Lys Ile Gly Asn Thr 
        115                 120                 125             


Leu Gly Ser Ala Ser Ser Ile Ser Gln Asn Ile Gly Lys Ala Asn Thr 
    130                 135                 140                 


Val Leu Gly Gly Ile Gln Ser Ile Leu Gly Ser Val Leu Ser Gly Val 
145                 150                 155                 160 


Asn Leu Asn Glu Leu Leu Gln Asn Lys Asp Pro Asn Gln Leu Glu Leu 
                165                 170                 175     


Ala Lys Ala Gly Leu Glu Leu Thr Asn Glu Leu Val Gly Asn Ile Ala 
            180                 185                 190         


Ser Ser Val Gln Thr Val Asp Ala Phe Ala Glu Gln Ile Ser Lys Leu 
        195                 200                 205             


Gly Ser His Leu Gln Asn Val Lys Gly Leu Gly Gly Leu Ser Asn Lys 
    210                 215                 220                 


Leu Gln Asn Leu Pro Asp Leu Gly Lys Ala Ser Leu Gly Leu Asp Ile 
225                 230                 235                 240 


Ile Ser Gly Leu Leu Ser Gly Ala Ser Ala Gly Leu Ile Leu Ala Asp 
                245                 250                 255     


Lys Glu Ala Ser Thr Glu Lys Lys Ala Ala Ala Gly Val Glu Phe Ala 
            260                 265                 270         


Asn Gln Ile Ile Gly Asn Val Thr Lys Ala Val Ser Ser Tyr Ile Leu 
        275                 280                 285             


Ala Gln Arg Val Ala Ser Gly Leu Ser Ser Thr Gly Pro Val Ala Ala 
    290                 295                 300                 


Leu Ile Ala Ser Thr Val Ala Leu Ala Val Ser Pro Leu Ser Phe Leu 
305                 310                 315                 320 


Asn Val Ala Asp Lys Phe Lys Gln Ala Asp Leu Ile Lys Ser Tyr Ser 
                325                 330                 335     


Glu Arg Phe Gln Lys Leu Gly Tyr Asp Gly Asp Arg Leu Leu Ala Asp 
            340                 345                 350         


Phe His Arg Glu Thr Gly Thr Ile Asp Ala Ser Val Thr Thr Ile Asn 
        355                 360                 365             


Thr Ala Leu Ala Ala Ile Ser Gly Gly Val Gly Ala Ala Ser Ala Gly 
    370                 375                 380                 


Ser Leu Val Gly Ala Pro Val Ala Leu Leu Val Ala Gly Val Thr Gly 
385                 390                 395                 400 


Leu Ile Thr Thr Ile Leu Glu Tyr Ser Lys Gln Ala Met Phe Glu His 
                405                 410                 415     


Val Ala Asn Lys Val His Asp Arg Ile Val Glu Trp Glu Lys Lys His 
            420                 425                 430         


Asn Lys Asn Tyr Phe Glu Gln Gly Tyr Asp Ser Arg His Leu Ala Asp 
        435                 440                 445             


Leu Gln Asp Asn Met Lys Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln 
    450                 455                 460                 


Ala Glu Arg Val Val Ala Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile 
465                 470                 475                 480 


Gly Asp Leu Ala Ala Ile Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly 
                485                 490                 495     


Lys Ala Tyr Val Asp Ala Phe Glu Glu Gly Gln His Gln Ser Tyr Asp 
            500                 505                 510         


Ser Ser Val Gln Leu Asp Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn 
        515                 520                 525             


Thr Asn Arg Lys Thr Gln Ser Val Leu Phe Arg Thr Pro Leu Leu Thr 
    530                 535                 540                 


Pro Gly Glu Glu Asn Arg Glu Arg Ile Gln Glu Gly Ala Asn Ser Tyr 
545                 550                 555                 560 


Ile Thr Lys Leu His Ile Gln Arg Val Asp Ser Trp Thr Val Thr Asp 
                565                 570                 575     


Gly Asp Ala Ser Ser Ser Val Asp Phe Thr Asn Val Val Gln Arg Ile 
            580                 585                 590         


Ala Val Lys Phe Asp Asp Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr 
        595                 600                 605             


Lys Ile Ile Ala Asn Leu Gly Ala Gly Asn Asp Asn Val Phe Val Gly 
    610                 615                 620                 


Ser Ser Thr Thr Val Ile Asp Gly Gly Asp Gly His Asp Arg Val His 
625                 630                 635                 640 


Tyr Ser Arg Gly Glu Tyr Gly Ala Leu Val Ile Asp Ala Thr Ala Glu 
                645                 650                 655     


Thr Glu Lys Gly Ser Tyr Ser Val Lys Arg Tyr Val Gly Asp Ser Lys 
            660                 665                 670         


Ala Leu His Glu Thr Ile Ala Thr His Gln Thr Asn Val Gly Ala Arg 
        675                 680                 685             


Glu Glu Lys Ile Glu Tyr Arg Arg Glu Asp Asp Arg Phe His Thr Gly 
    690                 695                 700                 


Tyr Thr Val Thr Asp Ser Leu Lys Ser Val Glu Glu Ile Ile Gly Ser 
705                 710                 715                 720 


Gln Phe Asn Asp Ile Phe Lys Gly Ser Gln Phe Asp Asp Val Phe His 
                725                 730                 735     


Gly Gly Asn Gly Val Asp Thr Ile Asp Gly Asn Asp Gly Asp Asp His 
            740                 745                 750         


Leu Phe Gly Gly Ala Gly Asp Asp Val Ile Asp Gly Gly Asn Gly Asn 
        755                 760                 765             


Asn Phe Leu Val Gly Gly Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys 
    770                 775                 780                 


Asp Asn Asp Ile Tyr Val His Lys Thr Gly Asp Gly Asn Asp Ser Ile 
785                 790                 795                 800 


Thr Asp Ser Gly Gly Gln Asp Lys Leu Ala Phe Ser Asp Val Asn Leu 
                805                 810                 815     


Lys Asp Leu Thr Phe Lys Lys Val Asp Ser Ser Leu Glu Ile Ile Asn 
            820                 825                 830         


Gln Lys Gly Glu Lys Val Arg Ile Gly Asn Trp Phe Leu Glu Asp Asp 
        835                 840                 845             


Leu Ala Ser Thr Val Ala Asn Tyr Lys Ala Thr Asn Asp Arg Lys Ile 
    850                 855                 860                 


Glu Glu Ile Ile Gly Lys Gly Gly Glu Arg Ile Thr Ser Glu Gln Val 
865                 870                 875                 880 


Asp Lys Leu Ile Lys Glu Gly Asn Asn Gln Ile Ser Ala Glu Ala Leu 
                885                 890                 895     


Ser Lys Val Val Asn Asp Tyr Asn Thr Ser Lys Asp Arg Gln Asn Val 
            900                 905                 910         


Ser Asn Ser Leu Ala Lys Leu Ile Ser Ser Val Gly Ser Phe Thr Ser 
        915                 920                 925             


Ser Ser Asp Phe Arg Asn Asn Leu Gly Thr Tyr Val Pro Ser Ser Ile 
    930                 935                 940                 


Asp Val Ser Asn Asn Ile Gln Leu Ala Arg Ala Ala 
945                 950                 955     


<210>  6
<211>  1052
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APP ApxIIIA K571A K702A

<400>  6

Met Ser Thr Trp Ser Ser Met Leu Ala Asp Leu Lys Lys Arg Ala Glu 
1               5                   10                  15      


Glu Ala Lys Arg Gln Ala Lys Lys Gly Tyr Asp Val Thr Lys Asn Gly 
            20                  25                  30          


Leu Gln Tyr Gly Val Ser Gln Ala Lys Leu Gln Ala Leu Ala Ala Gly 
        35                  40                  45              


Lys Ala Val Gln Lys Tyr Gly Asn Lys Leu Val Leu Val Ile Pro Lys 
    50                  55                  60                  


Glu Tyr Asp Gly Ser Val Gly Asn Gly Phe Phe Asp Leu Val Lys Ala 
65                  70                  75                  80  


Ala Glu Glu Leu Gly Ile Gln Val Lys Tyr Val Asn Arg Asn Glu Leu 
                85                  90                  95      


Glu Val Ala His Lys Ser Leu Gly Thr Ala Asp Gln Phe Leu Gly Leu 
            100                 105                 110         


Thr Glu Arg Gly Leu Thr Leu Phe Ala Pro Gln Leu Asp Gln Phe Leu 
        115                 120                 125             


Gln Lys His Ser Lys Ile Ser Asn Val Val Gly Ser Ser Thr Gly Asp 
    130                 135                 140                 


Ala Val Ser Lys Leu Ala Lys Ser Gln Thr Ile Ile Ser Gly Ile Gln 
145                 150                 155                 160 


Ser Val Leu Gly Thr Val Leu Ala Gly Ile Asn Leu Asn Glu Ala Ile 
                165                 170                 175     


Ile Ser Gly Gly Ser Glu Leu Glu Leu Ala Glu Ala Gly Val Ser Leu 
            180                 185                 190         


Ala Ser Glu Leu Val Ser Asn Ile Ala Lys Gly Thr Thr Thr Ile Asp 
        195                 200                 205             


Ala Phe Thr Thr Gln Ile Gln Asn Phe Gly Lys Leu Val Glu Asn Ala 
    210                 215                 220                 


Lys Gly Leu Gly Gly Val Gly Arg Gln Leu Gln Asn Ile Ser Gly Ser 
225                 230                 235                 240 


Ala Leu Ser Lys Thr Gly Leu Gly Leu Asp Ile Ile Ser Ser Leu Leu 
                245                 250                 255     


Ser Gly Val Thr Ala Ser Phe Ala Leu Ala Asn Lys Asn Ala Ser Thr 
            260                 265                 270         


Ser Thr Lys Val Ala Ala Gly Phe Glu Leu Ser Asn Gln Val Ile Gly 
        275                 280                 285             


Gly Ile Thr Lys Ala Val Ser Ser Tyr Ile Leu Ala Gln Arg Leu Ala 
    290                 295                 300                 


Ala Gly Leu Ser Thr Thr Gly Pro Ala Ala Ala Leu Ile Ala Ser Ser 
305                 310                 315                 320 


Ile Ser Leu Ala Ile Ser Pro Leu Ala Phe Leu Arg Val Ala Asp Asn 
                325                 330                 335     


Phe Asn Arg Ser Lys Glu Ile Gly Glu Phe Ala Glu Arg Phe Lys Lys 
            340                 345                 350         


Leu Gly Tyr Asp Gly Asp Lys Leu Leu Ser Glu Phe Tyr His Glu Ala 
        355                 360                 365             


Gly Thr Ile Asp Ala Ser Ile Thr Thr Ile Ser Thr Ala Leu Ser Ala 
    370                 375                 380                 


Ile Ala Ala Gly Thr Ala Ala Ala Ser Ala Gly Ala Leu Val Gly Ala 
385                 390                 395                 400 


Pro Ile Thr Leu Leu Val Thr Gly Ile Thr Gly Leu Ile Ser Gly Ile 
                405                 410                 415     


Leu Glu Phe Ser Lys Gln Pro Met Leu Asp His Val Ala Ser Lys Ile 
            420                 425                 430         


Gly Asn Lys Ile Asp Glu Trp Glu Lys Lys Tyr Gly Lys Asn Tyr Phe 
        435                 440                 445             


Glu Asn Gly Tyr Asp Ala Arg His Lys Ala Phe Leu Glu Asp Ser Phe 
    450                 455                 460                 


Ser Leu Leu Ser Ser Phe Asn Lys Gln Tyr Glu Thr Glu Arg Ala Val 
465                 470                 475                 480 


Leu Ile Thr Gln Gln Arg Trp Asp Glu Tyr Ile Gly Glu Leu Ala Gly 
                485                 490                 495     


Ile Thr Gly Lys Gly Asp Lys Leu Ser Ser Gly Lys Ala Tyr Val Asp 
            500                 505                 510         


Tyr Phe Gln Glu Gly Lys Leu Leu Glu Lys Lys Pro Asp Asp Phe Ser 
        515                 520                 525             


Lys Val Val Phe Asp Pro Thr Lys Gly Glu Ile Asp Ile Ser Asn Ser 
    530                 535                 540                 


Gln Thr Ser Thr Leu Leu Lys Phe Val Thr Pro Leu Leu Thr Pro Gly 
545                 550                 555                 560 


Thr Glu Ser Arg Glu Arg Thr Gln Thr Gly Ala Tyr Glu Tyr Ile Thr 
                565                 570                 575     


Lys Leu Val Val Lys Gly Lys Asp Lys Trp Val Val Asn Gly Val Lys 
            580                 585                 590         


Asp Lys Gly Ala Val Tyr Asp Tyr Thr Asn Leu Ile Gln His Ala His 
        595                 600                 605             


Ile Ser Ser Ser Val Ala Arg Gly Glu Glu Tyr Arg Glu Val Arg Leu 
    610                 615                 620                 


Val Ser His Leu Gly Asn Gly Asn Asp Lys Val Phe Leu Ala Ala Gly 
625                 630                 635                 640 


Ser Ala Glu Ile His Ala Gly Glu Gly His Asp Val Val Tyr Tyr Asp 
                645                 650                 655     


Lys Thr Asp Thr Gly Leu Leu Val Ile Asp Gly Thr Lys Ala Thr Glu 
            660                 665                 670         


Gln Gly Arg Tyr Ser Val Thr Arg Glu Leu Ser Gly Ala Thr Lys Ile 
        675                 680                 685             


Leu Arg Glu Val Ile Lys Asn Gln Lys Ser Ala Val Gly Ala Arg Glu 
    690                 695                 700                 


Glu Thr Leu Glu Tyr Arg Asp Tyr Glu Leu Thr Gln Ser Gly Asn Ser 
705                 710                 715                 720 


Asn Leu Lys Ala His Asp Glu Leu His Ser Val Glu Glu Ile Ile Gly 
                725                 730                 735     


Ser Asn Gln Arg Asp Glu Phe Lys Gly Ser Lys Phe Arg Asp Ile Phe 
            740                 745                 750         


His Gly Ala Asp Gly Asp Asp Leu Leu Asn Gly Asn Asp Gly Asp Asp 
        755                 760                 765             


Ile Leu Tyr Gly Asp Lys Gly Asn Asp Glu Leu Arg Gly Asp Asn Gly 
    770                 775                 780                 


Asn Asp Gln Leu Tyr Gly Gly Glu Gly Asn Asp Lys Leu Leu Gly Gly 
785                 790                 795                 800 


Asn Gly Asn Asn Tyr Leu Ser Gly Gly Asp Gly Asn Asp Glu Leu Gln 
                805                 810                 815     


Val Leu Gly Asn Gly Phe Asn Val Leu Arg Gly Gly Lys Gly Asp Asp 
            820                 825                 830         


Lys Leu Tyr Gly Ser Ser Gly Ser Asp Leu Leu Asp Gly Gly Glu Gly 
        835                 840                 845             


Asn Asp Tyr Leu Glu Gly Gly Asp Gly Ser Asp Phe Tyr Val Tyr Arg 
    850                 855                 860                 


Ser Thr Ser Gly Asn His Thr Ile Tyr Asp Gln Gly Lys Ser Ser Asp 
865                 870                 875                 880 


Leu Asp Lys Leu Tyr Leu Ser Asp Phe Ser Phe Asp Arg Leu Leu Val 
                885                 890                 895     


Glu Lys Val Asp Asp Asn Leu Val Leu Arg Ser Asn Glu Ser Ser His 
            900                 905                 910         


Asn Asn Gly Val Leu Thr Ile Lys Asp Trp Phe Lys Glu Gly Asn Lys 
        915                 920                 925             


Tyr Asn His Lys Ile Glu Gln Ile Val Asp Lys Asn Gly Arg Lys Leu 
    930                 935                 940                 


Thr Ala Glu Asn Leu Gly Thr Tyr Phe Lys Asn Ala Pro Lys Ala Asp 
945                 950                 955                 960 


Asn Leu Leu Asn Tyr Ala Thr Lys Glu Asp Gln Asn Glu Ser Asn Leu 
                965                 970                 975     


Ser Ser Leu Lys Thr Glu Leu Ser Lys Ile Ile Thr Asn Ala Gly Asn 
            980                 985                 990         


Phe Gly Val Ala Lys Gln Gly Asn  Thr Gly Ile Asn Thr  Ala Ala Leu 
        995                 1000                 1005             


Asn Asn  Glu Val Asn Lys Ile  Ile Ser Ser Ala Asn  Thr Phe Ala 
    1010                 1015                 1020             


Thr Ser  Gln Leu Gly Gly Ser  Gly Met Gly Thr Leu  Pro Ser Thr 
    1025                 1030                 1035             


Asn Val  Asn Ser Met Met Leu  Gly Asn Leu Ala Arg  Ala Ala 
    1040                 1045                 1050         


<210>  7
<211>  363
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  7

Gln Gly Tyr Asp Ser Arg His Leu Ala Asp Leu Gln Asp Asn Met Lys 
1               5                   10                  15      


Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln Ala Glu Arg Val Val Ala 
            20                  25                  30          


Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile Gly Asp Leu Ala Ala Ile 
        35                  40                  45              


Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly Lys Ala Tyr Val Asp Ala 
    50                  55                  60                  


Phe Glu Glu Gly Gln His Gln Ser Tyr Asp Ser Ser Val Gln Leu Asp 
65                  70                  75                  80  


Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn Thr Asn Arg Lys Thr Gln 
                85                  90                  95      


Ser Val Leu Phe Arg Thr Pro Leu Leu Thr Pro Gly Glu Glu Asn Arg 
            100                 105                 110         


Glu Arg Ile Gln Glu Gly Lys Asn Ser Tyr Ile Thr Lys Leu His Ile 
        115                 120                 125             


Gln Arg Val Asp Ser Trp Thr Val Thr Asp Gly Asp Ala Ser Ser Ser 
    130                 135                 140                 


Val Asp Phe Thr Asn Val Val Gln Arg Ile Ala Val Lys Phe Asp Asp 
145                 150                 155                 160 


Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr Lys Ile Ile Ala Asn Leu 
                165                 170                 175     


Gly Ala Gly Asn Asp Asn Val Phe Val Gly Ser Ser Thr Thr Val Ile 
            180                 185                 190         


Asp Gly Gly Asp Gly His Asp Arg Val His Tyr Ser Arg Gly Glu Tyr 
        195                 200                 205             


Gly Ala Leu Val Ile Asp Ala Thr Ala Glu Thr Glu Lys Gly Ser Tyr 
    210                 215                 220                 


Ser Val Lys Arg Tyr Val Gly Asp Ser Lys Ala Leu His Glu Thr Ile 
225                 230                 235                 240 


Ala Thr His Gln Thr Asn Val Gly Asn Arg Glu Glu Lys Ile Glu Tyr 
                245                 250                 255     


Arg Arg Glu Asp Asp Arg Phe His Thr Gly Tyr Thr Val Thr Asp Ser 
            260                 265                 270         


Leu Lys Ser Val Glu Glu Ile Ile Gly Ser Gln Phe Asn Asp Ile Phe 
        275                 280                 285             


Lys Gly Ser Gln Phe Asp Asp Val Phe His Gly Gly Asn Gly Val Asp 
    290                 295                 300                 


Thr Ile Asp Gly Asn Asp Gly Asp Asp His Leu Phe Gly Gly Ala Gly 
305                 310                 315                 320 


Asp Asp Val Ile Asp Gly Gly Asn Gly Asn Asn Phe Leu Val Gly Gly 
                325                 330                 335     


Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys Asp Asn Asp Ile Tyr Val 
            340                 345                 350         


His Lys Thr Gly Asp Gly Asn Asp Ser Ile Thr 
        355                 360             


<210>  8
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APP truncated ApxIIA K557A N687A

<400>  8

Gln Gly Tyr Asp Ser Arg His Leu Ala Asp Leu Gln Asp Asn Met Lys 
1               5                   10                  15      


Phe Leu Ile Asn Leu Asn Lys Glu Leu Gln Ala Glu Arg Val Val Ala 
            20                  25                  30          


Ile Thr Gln Gln Arg Trp Asp Asn Gln Ile Gly Asp Leu Ala Ala Ile 
        35                  40                  45              


Ser Arg Arg Thr Asp Lys Ile Ser Ser Gly Lys Ala Tyr Val Asp Ala 
    50                  55                  60                  


Phe Glu Glu Gly Gln His Gln Ser Tyr Asp Ser Ser Val Gln Leu Asp 
65                  70                  75                  80  


Asn Lys Asn Gly Ile Ile Asn Ile Ser Asn Thr Asn Arg Lys Thr Gln 
                85                  90                  95      


Ser Val Leu Phe Arg Thr Pro Leu Leu Thr Pro Gly Glu Glu Asn Arg 
            100                 105                 110         


Glu Arg Ile Gln Glu Gly Ala Asn Ser Tyr Ile Thr Lys Leu His Ile 
        115                 120                 125             


Gln Arg Val Asp Ser Trp Thr Val Thr Asp Gly Asp Ala Ser Ser Ser 
    130                 135                 140                 


Val Asp Phe Thr Asn Val Val Gln Arg Ile Ala Val Lys Phe Asp Asp 
145                 150                 155                 160 


Ala Gly Asn Ile Ile Glu Ser Lys Asp Thr Lys Ile Ile Ala Asn Leu 
                165                 170                 175     


Gly Ala Gly Asn Asp Asn Val Phe Val Gly Ser Ser Thr Thr Val Ile 
            180                 185                 190         


Asp Gly Gly Asp Gly His Asp Arg Val His Tyr Ser Arg Gly Glu Tyr 
        195                 200                 205             


Gly Ala Leu Val Ile Asp Ala Thr Ala Glu Thr Glu Lys Gly Ser Tyr 
    210                 215                 220                 


Ser Val Lys Arg Tyr Val Gly Asp Ser Lys Ala Leu His Glu Thr Ile 
225                 230                 235                 240 


Ala Thr His Gln Thr Asn Val Gly Ala Arg Glu Glu Lys Ile Glu Tyr 
                245                 250                 255     


Arg Arg Glu Asp Asp Arg Phe His Thr Gly Tyr Thr Val Thr Asp Ser 
            260                 265                 270         


Leu Lys Ser Val Glu Glu Ile Ile Gly Ser Gln Phe Asn Asp Ile Phe 
        275                 280                 285             


Lys Gly Ser Gln Phe Asp Asp Val Phe His Gly Gly Asn Gly Val Asp 
    290                 295                 300                 


Thr Ile Asp Gly Asn Asp Gly Asp Asp His Leu Phe Gly Gly Ala Gly 
305                 310                 315                 320 


Asp Asp Val Ile Asp Gly Gly Asn Gly Asn Asn Phe Leu Val Gly Gly 
                325                 330                 335     


Thr Gly Asn Asp Ile Ile Ser Gly Gly Lys Asp Asn Asp Ile Tyr Val 
            340                 345                 350         


His Lys Thr Gly Asp Gly Asn Asp Ser Ile Thr 
        355                 360             


<210>  9
<211>  3069
<212>  DNA
<213>  Actinobacillus pleuropneumoniae

<400>  9
atggctaact ctcagctcga tagagtcaaa ggattgattg attcacttaa tcaacataca       60

aaaagtgcag ctaaatcagg tgccggcgca ttaaaaaatg gtttgggaca ggtgaagcaa      120

gcagggcaga aattaatttt atatattccg aaagattatc aagctagtac cggctcaagt      180

cttaatgatt tagtgaaagc ggcggaggct ttagggatcg aagtacatcg ctcggaaaaa      240

aacggtaccg cactagcgaa agaattattc ggtacaacgg aaaaactatt aggtttctcg      300

gaacgaggca tcgcattatt tgcacctcag tttgataagt tactgaataa gaaccaaaaa      360

ttaagtaaat cgctcggcgg ttcatcggaa gcattaggac aacgtttaaa taaaacgcaa      420

acggcacttt cagccttaca aagtttctta ggtacggcta ttgcgggtat ggatcttgat      480

agcctgcttc gtcgccgtag aaacggtgag gacgtcagtg gttcggaatt agctaaagcg      540

ggtgtggatc tagccgctca gttagtggat aacattgcaa gtgcaacggg tacggtggat      600

gcgtttgccg aacaattagg taaattgggc aatgccttat ctaacactcg cttaagcggt      660

ttagcaagta agttaaataa ccttccagat ttaagccttg caggacctgg gtttgatgcc      720

gtatcaggta tcttatctgt tgtttcggct tcattcattt taagtaataa agatgccgat      780

gcaggtacaa aagcggcggc aggtattgaa atctcaacta aaatcttagg caatatcggt      840

aaagcggttt ctcaatatat tattgcgcaa cgtgtggcgg caggcttatc cacaactgcg      900

gcaaccggtg gtttaatcgg ttcggtcgta gcattagcga ttagcccgct ttcgttctta      960

aatgttgcgg ataagtttga acgtgcgaaa cagcttgaac aatattcgga gcgctttaaa     1020

aagttcggtt atgaaggtga tagtttatta gcttcattct accgtgaaac cggtgcgatt     1080

gaagcggcat taaccacgat taacagtgtg ttaagtgcgg cttccgcagg tgttggggct     1140

gctgcaaccg gctcattagt cggtgcgccg gtagcagctt tagttagtgc aatcaccggt     1200

attatttcag gtattttaga tgcttctaaa caggcaatct tcgaacgagt tgcaacgaaa     1260

ttagcgaata agattgacga atgggagaaa aaacacggta aaaactattt tgaaaacggt     1320

tatgacgccc gccattccgc attcttagaa gatacctttg aattgttatc acaatacaat     1380

aaagagtatt cggtagagcg tgtcgttgct attacgcaac aacgttggga tgtcaatatc     1440

ggggaacttg ccggtatcac gcgtaaaggt gcggatgcga aaagcggtaa ggcttatgtc     1500

gatttctttg aagaaggaaa attgttagag aaagatccgg atcgttttga taaaaaagtg     1560

tttgatccgc ttgaaggcaa aatcgacctt tcttcaatta acaaaaccac tttattgaaa     1620

tttattacac cggtttttac cgcaggtgaa gagattcgtg agcgtaagca aaccggtaaa     1680

tacgaatata tgaccgaatt attcgttaaa ggtaaagaaa aatgggtggt aaccggtgtg     1740

cagtcacata atgcgattta tgactatacg aatcttatcc aattagcgat agataaaaaa     1800

ggtgaaaaac gtcaagtgac cattgaatct catttgggtg agaaaaatga tcgtatatat     1860

ctttcatccg gttcatctat cgtatatgcg ggtaacggac atgatgtagc atattacgat     1920

aaaaccgata caggttactt aacatttgac ggacaaagtg cacagaaagc cggtgaatat     1980

attgtcacta aagaacttaa agctgatgta aaagttttaa aagaagtggt taaaactcag     2040

gatatttcag ttggaaaacg cagtgaaaaa ttagaatatc gtgattatga gttaagccca     2100

ttcgaacttg ggaacggtat cagagctaaa gatgaattac attctgttga agaaattatc     2160

ggtagtaatc gtaaagacaa attctttggt agtcgcttta ccgatatttt ccatggtgcg     2220

aaaggcgatg atgaaatcta cggtaatgac ggccacgata tcttatacgg agacgacggt     2280

aatgatgtaa tccatggcgg tgacggtaac gaccatcttg ttggtggtaa cggaaacgac     2340

cgattaatcg gcggaaaagg taataatttc cttaatggcg gtgatggtga cgatgagttg     2400

caggtctttg agggtcaata caacgtatta ttaggtggtg cgggtaatga cattctgtat     2460

ggcagcgatg gtactaactt atttgacggt ggtgtaggca atgacaaaat ctacggtggt     2520

ttaggtaagg atatttatcg ctacagtaag gagtacggtc gtcatatcat tattgagaaa     2580

ggcggtgatg atgatacgtt attgttatcg gatcttagtt ttaaagatgt aggatttatc     2640

agaatcggtg atgatcttct tgtgaataaa agaatcggag gaacactgta ttaccatgaa     2700

gattacaatg ggaatgcgct cacgattaaa gattggttca aggaaggtaa agaaggacaa     2760

aataataaaa ttgaaaaaat cgttgataaa gatggagctt atgttttaag ccaatatctg     2820

actgaactga cagctcctgg aagaggtatc aattacttta atgggttaga agaaaaattg     2880

tattatggag aaggatataa tgcacttcct caactcagaa aagatattga acaaatcatt     2940

tcatctactg gtgcacttac cggtgaacac ggacaagttt tagtgggagc aggcggtcca     3000

ttagcttaca gcaattcacc gaatagcata ccgaatgctt tcagtaatta tttaacacaa     3060

tctgcttaa                                                             3069


<210>  10
<211>  2871
<212>  DNA
<213>  Actinobacillus pleuropneumoniae

<400>  10
atgtcaaaaa tcactttgtc atcattaaaa tcgtccttac aacaaggatt gacaaatggg       60

aaaaacaagt taaatcaagc aggtacaaca ctgaagaatg gtttaactca aactggtcat      120

tctctacaga atggggctaa aaaattaatc ttatatattc ctcaaggcta tgattcgggt      180

caaggaaatg gaattcaaga tttagttaaa gctgctaatg atttaggtat tgaagtatgg      240

cgagaagaac gcagcaattt ggacattgca aaaactagct ttgatacaac tcagaaaatt      300

ctaggtttta ctgatagagg aattgtatta tttgcacctc agctagataa tttattaaag      360

aagaatccta aaattggcaa tacattagga agtgcttcta gcatctcaca aaatataggt      420

aaagccaata ctgtattagg tggtattcaa tctattttag gatctgtttt atctggagta      480

aatctgaatg aattacttca aaataaagat cctaatcaat tagaacttgc aaaagcaggg      540

ctagaactga ctaatgaatt agttggtaat attgctagct cggtgcaaac tgtagatgca      600

tttgcagaac aaatatctaa actaggttca catttacaga atgtgaaagg attaggagga      660

ttgagtaata aattacaaaa tctaccagat ctaggaaaag caagtttagg tttggacatt      720

atctctggtt tactttctgg agcatctgca ggtctcattt tagcagataa agaggcttca      780

acagaaaaga aagctgccgc aggtgtagaa tttgctaacc aaattatagg taatgtaaca      840

aaagcggtct catcttacat tcttgcccaa cgagtcgctt caggtttgtc ttcaactggt      900

cctgtcgctg cattaatcgc atctacagtt gcactagctg ttagccctct ttcattctta      960

aatgtagctg ataagtttaa acaagctgat ttaatcaaat catattctga acgcttccaa     1020

aaattaggat atgatggaga tcgtttatta gctgattttc accgtgagac aggaactatt     1080

gatgcttctg taacaacaat taacactgct ttagcagcta tctccggtgg agttggagct     1140

gcaagcgcgg gttctctagt cggagctcca gttgcgttac tcgttgctgg tgttacggga     1200

cttattacaa ctattctaga atattctaaa caagccatgt ttgaacatgt tgcaaataag     1260

gttcatgaca gaatagttga atgggagaaa aaacataata aaaactattt tgagcaaggt     1320

tatgattctc gtcatttagc tgatttacaa gacaatatga agtttcttat caatttaaat     1380

aaagaacttc aggctgaacg cgtagtagct attacccaac aaagatggga taaccaaatt     1440

ggagacctag cggcaattag ccgtagaacg gataaaattt ccagtggaaa agcttatgtg     1500

gatgcttttg aggaggggca acaccagtcc tacgattcat ccgtacagct agataacaaa     1560

aacggtatta ttaatattag taatacaaat agaaagacac aaagtgtttt attcagaact     1620

ccattactaa ctccaggtga agagaatcgg gaacgtattc aggaaggtaa aaattcttat     1680

attacaaaat tacatataca aagagttgac agttggactg taacagatgg tgatgctagc     1740

tcaagcgtag atttcactaa tgtagtacaa cgaatcgctg tgaaatttga tgatgcaggt     1800

aacattatcg aatctaaaga tactaaaatt atcgcaaatt taggtgctgg taacgataat     1860

gtatttgttg ggtcaagtac taccgttatt gatggcgggg acggacatga tcgagttcac     1920

tacagtagag gagaatatgg cgcattagtt attgatgcta cagccgagac agaaaaaggc     1980

tcatattcag taaaacgcta tgtcggagac agtaaagcat tacatgaaac aattgccacc     2040

caccaaacaa atgttggtaa tcgtgaagaa aaaattgaat atcgtcgtga agatgatcgt     2100

tttcatactg gttatactgt gacggactca ctcaaatcag ttgaagagat cattggttca     2160

caatttaatg atattttcaa aggaagccaa tttgatgatg tgttccatgg tggtaatggt     2220

gtagacacta ttgatggtaa cgatggtgac gatcatttat ttggtggcgc aggcgatgat     2280

gttatcgatg gaggaaacgg taacaatttc cttgttggag gaaccggtaa tgatattatc     2340

tcgggaggta aagataatga tatttatgtc cataaaacag gcgatggaaa tgattctatt     2400

acagactctg gcggacaaga taaactggca ttttcggatg taaatcttaa agacctcacc     2460

tttaagaaag tagattcttc tctcgaaatc attaatcaaa aaggagaaaa agttcgtatt     2520

gggaattggt tcttagaaga tgatttggct agcacagttg ctaactataa agctacgaat     2580

gaccgaaaaa ttgaggaaat tattggtaaa ggaggagaac gtattacatc agaacaagtt     2640

gataaactga ttaaggaggg taacaatcaa atctctgcag aagcattatc caaagttgtg     2700

aatgattaca atacgagtaa agatagacag aacgtatcta atagcttagc aaaattgatt     2760

tcttcagtcg ggagctttac gtcttcctca gactttagga ataatttagg aacatatgtt     2820

ccttcatcaa tagatgtctc gaataatatt caattagcta gagccgctta a              2871


<210>  11
<211>  3159
<212>  DNA
<213>  Actinobacillus pleuropneumoniae

<400>  11
atgagtactt ggtcaagcat gttagccgac ttaaaaaaac gggctgaaga agccaaaaga       60

caagccaaaa aaggctacga tgtaactaaa aatggtttgc aatatggggt gagtcaagca      120

aaattacaag cattagcagc tggtaaagcc gttcaaaagt acggtaataa attagtttta      180

gttattccaa aagagtatga cggaagtgtt ggtaacggtt tctttgattt agtaaaagca      240

gctgaggaat taggcattca agttaaatat gttaaccgta atgaattgga agttgcccat      300

aaaagtttag gtaccgcaga ccaattcttg ggtttaacag aacgtggact tactttattt      360

gcaccgcaac tagatcagtt cttacaaaaa cattcaaaaa tttctaacgt agtgggcagt      420

tctactggtg atgcagtaag taaacttgct aagagtcaaa ctattatttc aggaattcaa      480

tctgtattag gtactgtatt agcaggtatt aatcttaatg aagctattat tagtggcggt      540

tcagagctcg aattagctga agctggtgtt tctttagcct ctgagctcgt tagtaatatt      600

gctaaaggta caacaacaat agatgctttc actacacaaa tccagaactt tgggaaatta      660

gtggaaaatg ctaaagggtt aggtggtgtt ggccgccaat tacagaatat ttcaggttct      720

gcattaagca aaactggatt aggtttggat attatctcaa gcttactttc aggagtaact      780

gcaagttttg ctttagcgaa taagaatgct tcaacaagca ctaaagttgc tgctggcttt      840

gaactctcaa atcaagtaat tggtggtatt acgaaagcag tatcaagcta tattcttgca      900

cagcgtttag ctgctggttt atcaacgaca ggtcctgctg cagcactaat tgcgtctagt      960

atttctttag caatcagtcc attggcgttt ttacgtgtag ctgataattt taatcgttct     1020

aaagaaattg gcgaatttgc tgaacgtttc aaaaaattgg gctatgacgg cgataaacta     1080

ctttcagagt tttatcacga agctggtact attgatgcct caattactac aattagtaca     1140

gcactttctg ctatcgcagc tggaacggcc gccgcgagtg caggtgcatt agttggcgca     1200

ccaattactt tgttggttac tggtatcaca ggattaattt ctggtatttt agagttctct     1260

aaacaaccaa tgttagatca tgttgcatcg aaaattggta acaaaattga cgaatgggag     1320

aaaaaatacg gtaaaaatta cttcgagaat ggctatgatg ctcgtcataa agctttctta     1380

gaagattcat tctcattatt gtctagtttt aataaacaat atgaaactga aagagctgtt     1440

ttaattacac aacaacgttg ggatgaatat attggcgaac ttgcgggtat tactggcaaa     1500

ggtgacaaac tctctagtgg taaggcgtat gtagattact ttcaagaagg taaattatta     1560

gagaaaaaac ctgatgactt tagcaaagta gttttcgatc caactaaggg cgaaattgat     1620

atttcaaata gccaaacgtc aacgttgtta aaatttgtta cgccattatt aacaccaggt     1680

acagagtcac gtgaaagaac tcaaacaggt aaatatgaat atatcacgaa gttagttgta     1740

aaaggtaaag ataaatgggt tgttaatggc gttaaagata aaggtgccgt ttatgattat     1800

actaatttaa ttcaacatgc tcatattagt tcatcagtag cacgtggtga agaataccgt     1860

gaagttcgtt tggtatctca tctaggcaat ggtaatgaca aagtgttctt agctgcgggt     1920

tccgcagaaa ttcacgctgg tgaaggtcat gatgtggttt attatgataa aaccgataca     1980

ggtcttttag taattgatgg aaccaaagcg actgaacaag ggcgttattc tgttacgcgc     2040

gaattgagtg gtgctacaaa aatcctgaga gaagtaataa aaaatcaaaa atctgctgtt     2100

ggtaaacgtg aagaaacctt ggaatatcgt gattatgaat taacgcaatc aggtaatagt     2160

aacctaaaag cacatgatga attacattca gtagaagaaa ttattggaag taatcagaga     2220

gacgaattta aaggtagtaa attcagagat attttccatg gtgccgatgg tgatgatcta     2280

ttaaatggta atgatgggga tgatattcta tacggtgata aaggtaacga tgagttaaga     2340

ggtgataatg gtaacgacca actttatggt ggtgaaggta atgacaaact attaggaggt     2400

aatggcaata attacctcag tggtggtgat ggcaatgatg agcttcaagt cttaggcaat     2460

ggttttaatg tgcttcgtgg cggtaaaggc gatgataaac tttatggtag ctcaggttct     2520

gatttacttg atggtggaga aggtaatgat tatctagaag gaggcgatgg tagcgatttt     2580

tatgtttatc gttccacttc aggtaatcat actatttatg atcaaggtaa atctagtgat     2640

ttagataaac tatatttgtc tgatttttcc ttcgatcgtc ttcttgttga gaaagttgat     2700

gataaccttg tacttagaag taatgaaagt agtcataata atggagtact cacaatcaaa     2760

gactggttta aagaagggaa taaatataac cataaaattg aacaaattgt tgataaaaat     2820

ggtagaaaat tgacagcaga gaatttagga acttatttca aaaatgctcc aaaagctgac     2880

aatttgctta attatgcaac taaagaagat cagaatgaaa gcaatttatc ttcacttaaa     2940

actgaattaa gtaaaattat tactaatgca ggtaattttg gtgtggcaaa acaaggtaat     3000

actggaatca atacagctgc cttgaacaat gaagtgaata aaatcatttc ttctgctaat     3060

acctttgcta cttcacaatt gggtggctca gggatgggaa cattaccatc aacgaatgta     3120

aattcaatga tgctaggtaa cctagctaga gcagcttaa                            3159


<210>  12
<211>  3069
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APP ApxIA K560A K686A

<400>  12
atggctaact ctcagctcga tagagtcaaa ggattgattg attcacttaa tcaacataca       60

aaaagtgcag ctaaatcagg tgccggcgca ttaaaaaatg gtttgggaca ggtgaagcaa      120

gcagggcaga aattaatttt atatattccg aaagattatc aagctagtac cggctcaagt      180

cttaatgatt tagtgaaagc ggcggaggct ttagggatcg aagtacatcg ctcggaaaaa      240

aacggtaccg cactagcgaa agaattattc ggtacaacgg aaaaactatt aggtttctcg      300

gaacgaggca tcgcattatt tgcacctcag tttgataagt tactgaataa gaaccaaaaa      360

ttaagtaaat cgctcggcgg ttcatcggaa gcattaggac aacgtttaaa taaaacgcaa      420

acggcacttt cagccttaca aagtttctta ggtacggcta ttgcgggtat ggatcttgat      480

agcctgcttc gtcgccgtag aaacggtgag gacgtcagtg gttcggaatt agctaaagcg      540

ggtgtggatc tagccgctca gttagtggat aacattgcaa gtgcaacggg tacggtggat      600

gcgtttgccg aacaattagg taaattgggc aatgccttat ctaacactcg cttaagcggt      660

ttagcaagta agttaaataa ccttccagat ttaagccttg caggacctgg gtttgatgcc      720

gtatcaggta tcttatctgt tgtttcggct tcattcattt taagtaataa agatgccgat      780

gcaggtacaa aagcggcggc aggtattgaa atctcaacta aaatcttagg caatatcggt      840

aaagcggttt ctcaatatat tattgcgcaa cgtgtggcgg caggcttatc cacaactgcg      900

gcaaccggtg gtttaatcgg ttcggtcgta gcattagcga ttagcccgct ttcgttctta      960

aatgttgcgg ataagtttga acgtgcgaaa cagcttgaac aatattcgga gcgctttaaa     1020

aagttcggtt atgaaggtga tagtttatta gcttcattct accgtgaaac cggtgcgatt     1080

gaagcggcat taaccacgat taacagtgtg ttaagtgcgg cttccgcagg tgttggggct     1140

gctgcaaccg gctcattagt cggtgcgccg gtagcagctt tagttagtgc aatcaccggt     1200

attatttcag gtattttaga tgcttctaaa caggcaatct tcgaacgagt tgcaacgaaa     1260

ttagcgaata agattgacga atgggagaaa aaacacggta aaaactattt tgaaaacggt     1320

tatgacgccc gccattccgc attcttagaa gatacctttg aattgttatc acaatacaat     1380

aaagagtatt cggtagagcg tgtcgttgct attacgcaac aacgttggga tgtcaatatc     1440

ggggaacttg ccggtatcac gcgtaaaggt gcggatgcga aaagcggtaa ggcttatgtc     1500

gatttctttg aagaaggaaa attgttagag aaagatccgg atcgttttga taaaaaagtg     1560

tttgatccgc ttgaaggcaa aatcgacctt tcttcaatta acaaaaccac tttattgaaa     1620

tttattacac cggtttttac cgcaggtgaa gagattcgtg agcgtaagca aaccggtgca     1680

tacgaatata tgaccgaatt attcgttaaa ggtaaagaaa aatgggtggt aaccggtgtg     1740

cagtcacata atgcgattta tgactatacg aatcttatcc aattagcgat agataaaaaa     1800

ggtgaaaaac gtcaagtgac cattgaatct catttgggtg agaaaaatga tcgtatatat     1860

ctttcatccg gttcatctat cgtatatgcg ggtaacggac atgatgtagc atattacgat     1920

aaaaccgata caggttactt aacatttgac ggacaaagtg cacagaaagc cggtgaatat     1980

attgtcacta aagaacttaa agctgatgta aaagttttaa aagaagtggt taaaactcag     2040

gatatttcag ttggagcacg cagtgaaaaa ttagaatatc gtgattatga gttaagccca     2100

ttcgaacttg ggaacggtat cagagctaaa gatgaattac attctgttga agaaattatc     2160

ggtagtaatc gtaaagacaa attctttggt agtcgcttta ccgatatttt ccatggtgcg     2220

aaaggcgatg atgaaatcta cggtaatgac ggccacgata tcttatacgg agacgacggt     2280

aatgatgtaa tccatggcgg tgacggtaac gaccatcttg ttggtggtaa cggaaacgac     2340

cgattaatcg gcggaaaagg taataatttc cttaatggcg gtgatggtga cgatgagttg     2400

caggtctttg agggtcaata caacgtatta ttaggtggtg cgggtaatga cattctgtat     2460

ggcagcgatg gtactaactt atttgacggt ggtgtaggca atgacaaaat ctacggtggt     2520

ttaggtaagg atatttatcg ctacagtaag gagtacggtc gtcatatcat tattgagaaa     2580

ggcggtgatg atgatacgtt attgttatcg gatcttagtt ttaaagatgt aggatttatc     2640

agaatcggtg atgatcttct tgtgaataaa agaatcggag gaacactgta ttaccatgaa     2700

gattacaatg ggaatgcgct cacgattaaa gattggttca aggaaggtaa agaaggacaa     2760

aataataaaa ttgaaaaaat cgttgataaa gatggagctt atgttttaag ccaatatctg     2820

actgaactga cagctcctgg aagaggtatc aattacttta atgggttaga agaaaaattg     2880

tattatggag aaggatataa tgcacttcct caactcagaa aagatattga acaaatcatt     2940

tcatctactg gtgcacttac cggtgaacac ggacaagttt tagtgggagc aggcggtcca     3000

ttagcttaca gcaattcacc gaatagcata ccgaatgctt tcagtaatta tttaacacaa     3060

tctgcttaa                                                             3069


<210>  13
<211>  2871
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APP ApxIIA S148A K557A K686A

<400>  13
atgtcaaaaa tcactttgtc atcattaaaa tcgtccttac aacaaggatt gacaaatggg       60

aaaaacaagt taaatcaagc aggtacaaca ctgaagaatg gtttaactca aactggtcat      120

tctctacaga atggggctaa aaaattaatc ttatatattc ctcaaggcta tgattcgggt      180

caaggaaatg gaattcaaga tttagttaaa gctgctaatg atttaggtat tgaagtatgg      240

cgagaagaac gcagcaattt ggacattgca aaaactagct ttgatacaac tcagaaaatt      300

ctaggtttta ctgatagagg aattgtatta tttgcacctc agctagataa tttattaaag      360

aagaatccta aaattggcaa tacattagga agtgcttcta gcatctcaca aaatataggt      420

aaagccaata ctgtattagg tggtattcaa tctattttag gatctgtttt atctggagta      480

aatctgaatg aattacttca aaataaagat cctaatcaat tagaacttgc aaaagcaggg      540

ctagaactga ctaatgaatt agttggtaat attgctagct cggtgcaaac tgtagatgca      600

tttgcagaac aaatatctaa actaggttca catttacaga atgtgaaagg attaggagga      660

ttgagtaata aattacaaaa tctaccagat ctaggaaaag caagtttagg tttggacatt      720

atctctggtt tactttctgg agcatctgca ggtctcattt tagcagataa agaggcttca      780

acagaaaaga aagctgccgc aggtgtagaa tttgctaacc aaattatagg taatgtaaca      840

aaagcggtct catcttacat tcttgcccaa cgagtcgctt caggtttgtc ttcaactggt      900

cctgtcgctg cattaatcgc atctacagtt gcactagctg ttagccctct ttcattctta      960

aatgtagctg ataagtttaa acaagctgat ttaatcaaat catattctga acgcttccaa     1020

aaattaggat atgatggaga tcgtttatta gctgattttc accgtgagac aggaactatt     1080

gatgcttctg taacaacaat taacactgct ttagcagcta tctccggtgg agttggagct     1140

gcaagcgcgg gttctctagt cggagctcca gttgcgttac tcgttgctgg tgttacggga     1200

cttattacaa ctattctaga atattctaaa caagccatgt ttgaacatgt tgcaaataag     1260

gttcatgaca gaatagttga atgggagaaa aaacataata aaaactattt tgagcaaggt     1320

tatgattctc gtcatttagc tgatttacaa gacaatatga agtttcttat caatttaaat     1380

aaagaacttc aggctgaacg cgtagtagct attacccaac aaagatggga taaccaaatt     1440

ggagacctag cggcaattag ccgtagaacg gataaaattt ccagtggaaa agcttatgtg     1500

gatgcttttg aggaggggca acaccagtcc tacgattcat ccgtacagct agataacaaa     1560

aacggtatta ttaatattag taatacaaat agaaagacac aaagtgtttt attcagaact     1620

ccattactaa ctccaggtga agagaatcgg gaacgtattc aggaaggtgc aaattcttat     1680

attacaaaat tacatataca aagagttgac agttggactg taacagatgg tgatgctagc     1740

tcaagcgtag atttcactaa tgtagtacaa cgaatcgctg tgaaatttga tgatgcaggt     1800

aacattatcg aatctaaaga tactaaaatt atcgcaaatt taggtgctgg taacgataat     1860

gtatttgttg ggtcaagtac taccgttatt gatggcgggg acggacatga tcgagttcac     1920

tacagtagag gagaatatgg cgcattagtt attgatgcta cagccgagac agaaaaaggc     1980

tcatattcag taaaacgcta tgtcggagac agtaaagcat tacatgaaac aattgccacc     2040

caccaaacaa atgttggtgc tcgtgaagaa aaaattgaat atcgtcgtga agatgatcgt     2100

tttcatactg gttatactgt gacggactca ctcaaatcag ttgaagagat cattggttca     2160

caatttaatg atattttcaa aggaagccaa tttgatgatg tgttccatgg tggtaatggt     2220

gtagacacta ttgatggtaa cgatggtgac gatcatttat ttggtggcgc aggcgatgat     2280

gttatcgatg gaggaaacgg taacaatttc cttgttggag gaaccggtaa tgatattatc     2340

tcgggaggta aagataatga tatttatgtc cataaaacag gcgatggaaa tgattctatt     2400

acagactctg gcggacaaga taaactggca ttttcggatg taaatcttaa agacctcacc     2460

tttaagaaag tagattcttc tctcgaaatc attaatcaaa aaggagaaaa agttcgtatt     2520

gggaattggt tcttagaaga tgatttggct agcacagttg ctaactataa agctacgaat     2580

gaccgaaaaa ttgaggaaat tattggtaaa ggaggagaac gtattacatc agaacaagtt     2640

gataaactga ttaaggaggg taacaatcaa atctctgcag aagcattatc caaagttgtg     2700

aatgattaca atacgagtaa agatagacag aacgtatcta atagcttagc aaaattgatt     2760

tcttcagtcg ggagctttac gtcttcctca gactttagga ataatttagg aacatatgtt     2820

ccttcatcaa tagatgtctc gaataatatt caattagcta gagccgctta a              2871


<210>  14
<211>  3159
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APP ApxIIIA K571A K702A

<400>  14
atgagtactt ggtcaagcat gttagccgac ttaaaaaaac gggctgaaga agccaaaaga       60

caagccaaaa aaggctacga tgtaactaaa aatggtttgc aatatggggt gagtcaagca      120

aaattacaag cattagcagc tggtaaagcc gttcaaaagt acggtaataa attagtttta      180

gttattccaa aagagtatga cggaagtgtt ggtaacggtt tctttgattt agtaaaagca      240

gctgaggaat taggcattca agttaaatat gttaaccgta atgaattgga agttgcccat      300

aaaagtttag gtaccgcaga ccaattcttg ggtttaacag aacgtggact tactttattt      360

gcaccgcaac tagatcagtt cttacaaaaa cattcaaaaa tttctaacgt agtgggcagt      420

tctactggtg atgcagtaag taaacttgct aagagtcaaa ctattatttc aggaattcaa      480

tctgtattag gtactgtatt agcaggtatt aatcttaatg aagctattat tagtggcggt      540

tcagagctcg aattagctga agctggtgtt tctttagcct ctgagctcgt tagtaatatt      600

gctaaaggta caacaacaat agatgctttc actacacaaa tccagaactt tgggaaatta      660

gtggaaaatg ctaaagggtt aggtggtgtt ggccgccaat tacagaatat ttcaggttct      720

gcattaagca aaactggatt aggtttggat attatctcaa gcttactttc aggagtaact      780

gcaagttttg ctttagcgaa taagaatgct tcaacaagca ctaaagttgc tgctggcttt      840

gaactctcaa atcaagtaat tggtggtatt acgaaagcag tatcaagcta tattcttgca      900

cagcgtttag ctgctggttt atcaacgaca ggtcctgctg cagcactaat tgcgtctagt      960

atttctttag caatcagtcc attggcgttt ttacgtgtag ctgataattt taatcgttct     1020

aaagaaattg gcgaatttgc tgaacgtttc aaaaaattgg gctatgacgg cgataaacta     1080

ctttcagagt tttatcacga agctggtact attgatgcct caattactac aattagtaca     1140

gcactttctg ctatcgcagc tggaacggcc gccgcgagtg caggtgcatt agttggcgca     1200

ccaattactt tgttggttac tggtatcaca ggattaattt ctggtatttt agagttctct     1260

aaacaaccaa tgttagatca tgttgcatcg aaaattggta acaaaattga cgaatgggag     1320

aaaaaatacg gtaaaaatta cttcgagaat ggctatgatg ctcgtcataa agctttctta     1380

gaagattcat tctcattatt gtctagtttt aataaacaat atgaaactga aagagctgtt     1440

ttaattacac aacaacgttg ggatgaatat attggcgaac ttgcgggtat tactggcaaa     1500

ggtgacaaac tctctagtgg taaggcgtat gtagattact ttcaagaagg taaattatta     1560

gagaaaaaac ctgatgactt tagcaaagta gttttcgatc caactaaggg cgaaattgat     1620

atttcaaata gccaaacgtc aacgttgtta aaatttgtta cgccattatt aacaccaggt     1680

acagagtcac gtgaaagaac tcaaacaggt gcatatgaat atatcacgaa gttagttgta     1740

aaaggtaaag ataaatgggt tgttaatggc gttaaagata aaggtgccgt ttatgattat     1800

actaatttaa ttcaacatgc tcatattagt tcatcagtag cacgtggtga agaataccgt     1860

gaagttcgtt tggtatctca tctaggcaat ggtaatgaca aagtgttctt agctgcgggt     1920

tccgcagaaa ttcacgctgg tgaaggtcat gatgtggttt attatgataa aaccgataca     1980

ggtcttttag taattgatgg aaccaaagcg actgaacaag ggcgttattc tgttacgcgc     2040

gaattgagtg gtgctacaaa aatcctgaga gaagtaataa aaaatcaaaa atctgctgtt     2100

ggtgcacgtg aagaaacctt ggaatatcgt gattatgaat taacgcaatc aggtaatagt     2160

aacctaaaag cacatgatga attacattca gtagaagaaa ttattggaag taatcagaga     2220

gacgaattta aaggtagtaa attcagagat attttccatg gtgccgatgg tgatgatcta     2280

ttaaatggta atgatgggga tgatattcta tacggtgata aaggtaacga tgagttaaga     2340

ggtgataatg gtaacgacca actttatggt ggtgaaggta atgacaaact attaggaggt     2400

aatggcaata attacctcag tggtggtgat ggcaatgatg agcttcaagt cttaggcaat     2460

ggttttaatg tgcttcgtgg cggtaaaggc gatgataaac tttatggtag ctcaggttct     2520

gatttacttg atggtggaga aggtaatgat tatctagaag gaggcgatgg tagcgatttt     2580

tatgtttatc gttccacttc aggtaatcat actatttatg atcaaggtaa atctagtgat     2640

ttagataaac tatatttgtc tgatttttcc ttcgatcgtc ttcttgttga gaaagttgat     2700

gataaccttg tacttagaag taatgaaagt agtcataata atggagtact cacaatcaaa     2760

gactggttta aagaagggaa taaatataac cataaaattg aacaaattgt tgataaaaat     2820

ggtagaaaat tgacagcaga gaatttagga acttatttca aaaatgctcc aaaagctgac     2880

aatttgctta attatgcaac taaagaagat cagaatgaaa gcaatttatc ttcacttaaa     2940

actgaattaa gtaaaattat tactaatgca ggtaattttg gtgtggcaaa acaaggtaat     3000

actggaatca atacagctgc cttgaacaat gaagtgaata aaatcatttc ttctgctaat     3060

acctttgcta cttcacaatt gggtggctca gggatgggaa cattaccatc aacgaatgta     3120

aattcaatga tgctaggtaa cctagctaga gcagcttaa                            3159


<210>  15
<211>  2446
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pEX-A258

<400>  15
gtggcagctc tagagctagc gaattctttg gtgaaattgt tatccgctca caattccaca       60

caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact      120

cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct      180

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc      240

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca      300

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg      360

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca      420

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa      480

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc      540

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc      600

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct      660

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg      720

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag      780

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta      840

cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg      900

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt      960

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     1020

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     1080

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     1140

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     1200

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     1260

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaacc     1320

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     1380

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     1440

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     1500

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     1560

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     1620

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     1680

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     1740

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     1800

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     1860

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     1920

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     1980

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     2040

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     2100

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     2160

acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac     2220

gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct     2280

cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg     2340

cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat     2400

tgtactgaga gtttggcaat tggtcgacct cgagggcgcg cccgta                    2446


<210>  16
<211>  4751
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pQE-80L

<400>  16
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca       60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga      120

ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct      180

gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag      240

aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga      300

atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc      360

actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt      420

cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta      480

aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc      540

ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg      600

gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc      660

tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg      720

tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc      780

tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac      840

ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg      900

ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt      960

aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt     1020

tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg     1080

aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct     1140

cctgagtagg acaaatccgc cctctagatt acgtgcagtc gatgataagc tgtcaaacat     1200

gagaattgtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct     1260

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     1320

ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag     1380

ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg     1440

ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc     1500

ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt     1560

aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac     1620

gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc     1680

ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag     1740

acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc     1800

caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact     1860

gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc     1920

ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg     1980

ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat     2040

cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg     2100

cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc     2160

cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac     2220

tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg     2280

ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac     2340

cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc     2400

gatggtgtcg gaatttcggg cagcgttggg tcctggccac gggtgcgcat gatctagagc     2460

tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg     2520

gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg     2580

ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat     2640

actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     2700

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc     2760

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     2820

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     2880

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     2940

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     3000

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     3060

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     3120

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     3180

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     3240

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     3300

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     3360

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     3420

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     3480

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     3540

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     3600

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     3660

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     3720

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     3780

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     3840

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     3900

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     3960

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     4020

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     4080

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     4140

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     4200

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     4260

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     4320

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     4380

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     4440

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     4500

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     4560

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     4620

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     4680

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     4740

ttcgtcttca c                                                          4751


<210>  17
<211>  3431
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pQE-60

<400>  17
ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca       60

attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatggga      120

ggatccagat ctcatcacca tcaccatcac taagcttaat tagctgagct tggactcctg      180

ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc      240

cgccgggcgt tttttattgg tgagaatcca agctagcttg gcgagatttt caggagctaa      300

ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca      360

tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt      420

tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc      480

ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat      540

gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga      600

gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct      660

acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg      720

gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga      780

tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgc atgggcaaat      840

attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct      900

gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc      960

agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga     1020

ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt     1080

tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctctagagc     1140

tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg     1200

gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg     1260

ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat     1320

actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg     1380

aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc     1440

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     1500

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     1560

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     1620

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     1680

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     1740

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     1800

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     1860

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     1920

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     1980

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     2040

ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     2100

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     2160

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     2220

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     2280

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     2340

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     2400

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     2460

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     2520

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     2580

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     2640

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     2700

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     2760

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     2820

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     2880

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     2940

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     3000

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     3060

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     3120

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     3180

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     3240

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     3300

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     3360

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     3420

ttcgtcttca c                                                          3431


<210>  18
<211>  2938
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dfrA14sacB cassette

<400>  18
gttaatgccg tctgaagtgc gaagcggcat cagagcagat tgtactgaga gtgcaccata       60

tggtcgacct cgagttaatt aacgtatgcg gccgctttag actatttaaa taatattatt      120

taaattcttt actatagtgt acaatacaca cagtccatta accaaaataa aaggaggaat      180

taggatgaga accttgaaag tatcattgat agctgcgaaa gcgaaaaacg gcgtgattgg      240

ttgcggtcca gacataccct ggtccgcgaa aggggagcag ctacttttta aagcattgac      300

ctacaatcag tggcttctgg tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa      360

taggaaatac gcggtcgtta cccgctcagg ttggacatca aatgatgaca atgtagttgt      420

atttcagtca atcgaagagg ccatggacag gctagctgaa ttcaccggtc acgttatagt      480

gtctggtggc ggagaaattt accgagaaac attacccatg gcctctacgc tccacttatc      540

gacgatcgac atcgagccag agggggatgt tttcttcccg agtattccaa ataccttcga      600

agttgttttt gagcaacact ttacttcaaa cattaactat tgctatcaaa tttggaaaaa      660

gggttaatgc cgtctgaagt gcggtacaag cggtagaacc tgccccgtta gttgaaaccg      720

cttgttatgc atgcatggga tccgcgaatc ccgcggccat ggcggccggg agcatgcgac      780

gtcgggccca ttgggatccg cttttacagc gattgcagaa tgattgaatt gtaaacttta      840

gagctttata ttttgtttaa tggtattata tttacttata tttatgattc ttagttttta      900

ttgtaaatta aagtgtttat ttattgtatt ttaagtataa gatccttttt aacccatcac      960

atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt tctgaattgt     1020

gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca gagaatgaaa     1080

agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt ttatgatttt     1140

ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct aatagaatga     1200

ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc taaaatgtgt     1260

aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt tttttattgt     1320

gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt     1380

acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat     1440

taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga     1500

accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc     1560

aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa     1620

ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg     1680

ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc     1740

ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta     1800

ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg     1860

attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg     1920

gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga     1980

caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg     2040

attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg     2100

atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag     2160

ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag     2220

gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag     2280

aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc     2340

tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg     2400

catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat     2460

ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg     2520

atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca     2580

aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac     2640

acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca     2700

gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg aacatcaaag     2760

gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca     2820

aataaaaacg caaaagaaaa tgccgatatc ctattggcat tttcttttat ttcttatcaa     2880

cataaaggtg aatcccatac ctagagctgc acgcgagaca tgaacgtgca actgcttc       2938


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker tri_OE_for

<400>  19
gttaatgccg tctgaagtgc gaag                                              24


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker sac_OE_rev

<400>  20
gaagcagttg cacgttcatg tctc                                              24


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  left_flank_for primer

<400>  21
attgggtacc gagctcgc                                                     18


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tri_OE_rev primer

<400>  22
cttcgcactt cagacggcat taac                                              24


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sac_OE_for primer

<400>  23
gagacatgaa cgtgcaactg cttc                                              24


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  right_flank_rev primer

<400>  24
ccatttcaca caggaattcg gatc                                              24


<210>  25
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  for primer

<400>  25
aaacaagcgg tccggatctt ggaatttcgg c                                      31


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rev primer

<400>  26
tgccttcaag cggatcaaac ac                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  for primer

<400>  27
tcgaacttgg gaacggtatc ag                                                22


<210>  28
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rev primer

<400>  28
ttacaagcgg tactttgcca gcttacctac gatg                                   34


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIA_mut_for_OE

<400>  29
gtgtttgatc cgcttgaagg ca                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIA_mut_rev_OE

<400>  30
ctgataccgt tcccaagttc ga                                                22


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  left_flank_for_USS

<400>  31
atccacaagc ggtcatctgg c                                                 21


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sxy_TS_LF_for

<400>  32
gtaccgcttg ttaaatgatt acacc                                             25


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sxy_TS_LF_rev1

<400>  33
ggcattaact tagttagcct gtgagatagc                                        30


<210>  34
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sxy_TS_LF_rev2

<400>  34
cttcgcactt cagacggcat taacttagtt agcctgtgag                             40


<210>  35
<211>  3988
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta_apxIA_dfrA14sacB

<400>  35
attgggtacc gagctcgcgg ccgcaccggc tcattagtcg gtgcgccggt agcagcttta       60

gttagtgcaa tcaccggtat tatttcaggt attttagatg cttctaaaca ggcaatcttc      120

gaacgagttg caacgaaatt agcgaataag attgacgaat gggagaaaaa acacggtaaa      180

aactattttg aaaacggtta tgacgcccgc cattccgcat tcttagaaga tacctttgaa      240

ttgttatcac aatacaataa agagtattcg gtagagcgtg tcgttgctat tacgcaacaa      300

cgttgggatg tcaatatcgg ggaacttgcc ggtatcacgc gtaaaggtgc ggatgcgaaa      360

agcggtaagg cttatgtcga tttctttgaa gaaggaaaat tgttagagaa agatccggat      420

cgttttgata aaaaagtgtt tgatccgctt gaaggcaaaa tcgacctttc ttcaattaac      480

aaaaccactt tattgaaatt tattacaccg gtttttaccg caggtgttaa tgccgtctga      540

agtgcgaagc ggcatcagag cagattgtac tgagagtgca ccatatggtc gacctcgagt      600

taattaacgt atgcggccgc tttagactat ttaaataata ttatttaaat tctttactat      660

agtgtacaat acacacagtc cattaaccaa aataaaagga ggaattagga tgagaacctt      720

gaaagtatca ttgatagctg cgaaagcgaa aaacggcgtg attggttgcg gtccagacat      780

accctggtcc gcgaaagggg agcagctact ttttaaagca ttgacctaca atcagtggct      840

tctggtgggt cgcaagacgt ttgaatctat gggcgcactc cccaatagga aatacgcggt      900

cgttacccgc tcaggttgga catcaaatga tgacaatgta gttgtatttc agtcaatcga      960

agaggccatg gacaggctag ctgaattcac cggtcacgtt atagtgtctg gtggcggaga     1020

aatttaccga gaaacattac ccatggcctc tacgctccac ttatcgacga tcgacatcga     1080

gccagagggg gatgttttct tcccgagtat tccaaatacc ttcgaagttg tttttgagca     1140

acactttact tcaaacatta actattgcta tcaaatttgg aaaaagggtt aatgccgtct     1200

gaagtgcggt acaagcggta gaacctgccc cgttagttga aaccgcttgt tatgcatgca     1260

tgggatccgc gaatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccattggg     1320

atccgctttt acagcgattg cagaatgatt gaattgtaaa ctttagagct ttatattttg     1380

tttaatggta ttatatttac ttatatttat gattcttagt ttttattgta aattaaagtg     1440

tttatttatt gtattttaag tataagatcc tttttaaccc atcacatata cctgccgttc     1500

actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac     1560

tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt     1620

ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga     1680

ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat     1740

cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta     1800

tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc     1860

catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga     1920

catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg     1980

cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata     2040

aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc     2100

aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt     2160

ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg     2220

caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg     2280

acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa     2340

acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag     2400

accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat     2460

tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta     2520

acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct     2580

ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca     2640

gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat     2700

acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat     2760

ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc     2820

tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc     2880

taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa     2940

cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg     3000

actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg     3060

gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt     3120

taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc     3180

aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag     3240

acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg     3300

ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa     3360

gaaaatgccg atatcctatt ggcattttct tttatttctt atcaacataa aggtgaatcc     3420

catacctaga gctgcacgcg agacatgaac gtgcaactgc ttcccattcg aacttgggaa     3480

cggtatcaga gctaaagatg aattacattc tgttgaagaa attatcggta gtaatcgtaa     3540

agacaaattc tttggtagtc gctttaccga tattttccat ggtgcgaaag gcgatgatga     3600

aatctacggt aatgacggcc acgatatctt atacggagac gacggtaatg atgtaatcca     3660

tggcggtgac ggtaacgacc atcttgttgg tggtaacgga aacgaccgat taatcggcgg     3720

aaaaggtaat aatttcctta atggcggtga tggtgacgat gagttgcagg tctttgaggg     3780

tcaatacaac gtattattag gtggtgcggg taatgacatt ctgtatggca gcgatggtac     3840

taacttattt gacggtggtg taggcaatga caaaatctac ggtggtttag gtaaggatat     3900

ttatcgctac agtaaggagt acggtcgtca tatcattatt gagaaaggcg gtgatgatga     3960

tacggatccg aattcctgtg tgaaatgg                                        3988


<210>  36
<211>  1473
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIAmut

<400>  36
gaccgcggcc gcaccggctc attagtcggt gcgccggtag cagctttagt tagtgcaatc       60

accggtatta tttcaggtat tttagatgct tctaaacagg caatcttcga acgagttgca      120

acgaaattag cgaataagat tgacgaatgg gagaaaaaac acggtaaaaa ctattttgaa      180

aacggttatg acgcccgcca ttccgcattc ttagaagata cctttgaatt gttatcacaa      240

tacaataaag agtattcggt agagcgtgtc gttgctatta cgcaacaacg ttgggatgtc      300

aatatcgggg aacttgccgg tatcacgcgt aaaggtgcgg atgcgaaaag cggtaaggct      360

tatgtcgatt tctttgaaga aggaaaattg ttagagaaag atccggatcg ttttgataaa      420

aaagtgtttg atccgcttga aggcaaaatc gacctttctt caattaacaa aaccacttta      480

ttgaaattta ttacaccggt ttttaccgca ggtgaagaga ttcgtgagcg taagcaaacc      540

ggtgcatacg aatatatgac cgaattattc gttaaaggta aagaaaaatg ggtggtaacc      600

ggtgtgcagt cacataatgc gatttatgac tatacgaatc ttatccaatt agcgatagat      660

aaaaaaggtg aaaaacgtca agtgaccatt gaatctcatt tgggtgagaa aaatgatcgt      720

atatatcttt catccggttc atctatcgta tatgcgggta acggacatga tgtagcatat      780

tacgataaaa ccgatacagg ttacttaaca tttgacggac aaagtgcaca gaaagccggt      840

gaatatattg tcactaaaga acttaaagct gatgtaaaag ttttaaaaga agtggttaaa      900

actcaggata tttcagttgg agcacgcagt gaaaaattag aatatcgtga ttatgagtta      960

agcccattcg aacttgggaa cggtatcaga gctaaagatg aattacattc tgttgaagaa     1020

attatcggta gtaatcgtaa agacaaattc tttggtagtc gctttaccga tattttccat     1080

ggtgcgaaag gcgatgatga aatctacggt aatgacggcc acgatatctt atacggagac     1140

gacggtaatg atgtaatcca tggcggtgac ggtaacgacc atcttgttgg tggtaacgga     1200

aacgaccgat taatcggcgg aaaaggtaat aatttcctta atggcggtga tggtgacgat     1260

gagttgcagg tctttgaggg tcaatacaac gtattattag gtggtgcggg taatgacatt     1320

ctgtatggca gcgatggtac taacttattt gacggtggtg taggcaatga caaaatctac     1380

ggtggtttag gtaaggatat ttatcgctac agtaaggagt acggtcgtca tatcattatt     1440

gagaaaggcg gtgatgatga tacaagcggt ttg                                  1473


<210>  37
<211>  3987
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta_apxIIA_dfrA14sacB

<400>  37
attgggtacc gagctcgcgg ccgcgctgca agcgcgggtt ctctagtcgg agctccagtt       60

gcgttactcg ttgctggtgt tacgggactt attacaacta ttctagaata ttctaaacaa      120

gccatgtttg aacatgttgc aaataaggtt catgacagaa tagttgaatg ggagaaaaaa      180

cataataaaa actattttga gcaaggttat gattctcgtc atttagctga tttacaagac      240

aatatgaagt ttcttatcaa tttaaataaa gaacttcagg ctgaacgcgt agtagctatt      300

acccaacaaa gatgggataa ccaaattgga gacctagcgg caattagccg tagaacggat      360

aaaatttcca gtggaaaagc ttatgtggat gcttttgagg aggggcaaca ccagtcctac      420

gattcatccg tacagctaga taacaaaaac ggtattatta atattagtaa tacaaataga      480

aagacacaaa gtgttttatt cagaactcca ttactaactc caggtgttaa tgccgtctga      540

agtgcgaagc ggcatcagag cagattgtac tgagagtgca ccatatggtc gacctcgagt      600

taattaacgt atgcggccgc tttagactat ttaaataata ttatttaaat tctttactat      660

agtgtacaat acacacagtc cattaaccaa aataaaagga ggaattagga tgagaacctt      720

gaaagtatca ttgatagctg cgaaagcgaa aaacggcgtg attggttgcg gtccagacat      780

accctggtcc gcgaaagggg agcagctact ttttaaagca ttgacctaca atcagtggct      840

tctggtgggt cgcaagacgt ttgaatctat gggcgcactc cccaatagga aatacgcggt      900

cgttacccgc tcaggttgga catcaaatga tgacaatgta gttgtatttc agtcaatcga      960

agaggccatg gacaggctag ctgaattcac cggtcacgtt atagtgtctg gtggcggaga     1020

aatttaccga gaaacattac ccatggcctc tacgctccac ttatcgacga tcgacatcga     1080

gccagagggg gatgttttct tcccgagtat tccaaatacc ttcgaagttg tttttgagca     1140

acactttact tcaaacatta actattgcta tcaaatttgg aaaaagggtt aatgccgtct     1200

gaagtgcggt acaagcggta gaacctgccc cgttagttga aaccgcttgt tatgcatgca     1260

tgggatccgc gaatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccattggg     1320

atccgctttt acagcgattg cagaatgatt gaattgtaaa ctttagagct ttatattttg     1380

tttaatggta ttatatttac ttatatttat gattcttagt ttttattgta aattaaagtg     1440

tttatttatt gtattttaag tataagatcc tttttaaccc atcacatata cctgccgttc     1500

actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac     1560

tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt     1620

ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga     1680

ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat     1740

cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta     1800

tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc     1860

catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga     1920

catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg     1980

cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata     2040

aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc     2100

aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt     2160

ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg     2220

caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg     2280

acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa     2340

acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag     2400

accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat     2460

tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta     2520

acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct     2580

ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca     2640

gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat     2700

acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat     2760

ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc     2820

tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc     2880

taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa     2940

cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg     3000

actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg     3060

gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt     3120

taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc     3180

aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag     3240

acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg     3300

ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa     3360

gaaaatgccg atatcctatt ggcattttct tttatttctt atcaacataa aggtgaatcc     3420

catacctaga gctgcacgcg agacatgaac gtgcaactgc ttcgttttca tactggttat     3480

actgtgacgg actcactcaa atcagttgaa gagatcattg gttcacaatt taatgatatt     3540

ttcaaaggaa gccaatttga tgatgtgttc catggtggta atggtgtaga cactattgat     3600

ggtaacgatg gtgacgatca tttatttggt ggcgcaggcg atgatgttat cgatggagga     3660

aacggtaaca atttccttgt tggaggaacc ggtaatgata ttatctcggg aggtaaagat     3720

aatgatattt atgtccataa aacaggcgat ggaaatgatt ctattacaga ctctggcgga     3780

caagataaac tggcattttc ggatgtaaat cttaaagacc tcacctttaa gaaagtagat     3840

tcttctctcg aaatcattaa tcaaaaagga gaaaaagttc gtattgggaa ttggttctta     3900

gaagatgatt tggctagcac agttgctaac tataaagcta cgaatgaccg aaaaattgag     3960

gaagatccga attcctgtgt gaaatgg                                         3987


<210>  38
<211>  1483
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIIAmut

<400>  38
gaccgcggcc gcgctgcaag cgcgggttct ctagtcggag ctccagttgc gttactcgtt       60

gctggtgtta cgggacttat tacaactatt ctagaatatt ctaaacaagc catgtttgaa      120

catgttgcaa ataaggttca tgacagaata gttgaatggg agaaaaaaca taataaaaac      180

tattttgagc aaggttatga ttctcgtcat ttagctgatt tacaagacaa tatgaagttt      240

cttatcaatt taaataaaga acttcaggct gaacgcgtag tagctattac ccaacaaaga      300

tgggataacc aaattggaga cctagcggca attagccgta gaacggataa aatttccagt      360

ggaaaagctt atgtggatgc ttttgaggag gggcaacacc agtcctacga ttcatccgta      420

cagctagata acaaaaacgg tattattaat attagtaata caaatagaaa gacacaaagt      480

gttttattca gaactccatt actaactcca ggtgaagaga atcgggaacg tattcaggaa      540

ggtgcaaatt cttatattac aaaattacat atacaaagag ttgacagttg gactgtaaca      600

gatggtgatg ctagctcaag cgtagatttc actaatgtag tacaacgaat cgctgtgaaa      660

tttgatgatg caggtaacat tatcgaatct aaagatacta aaattatcgc aaatttaggt      720

gctggtaacg ataatgtatt tgttgggtca agtactaccg ttattgatgg cggggacgga      780

catgatcgag ttcactacag tagaggagaa tatggcgcat tagttattga tgctacagcc      840

gagacagaaa aaggctcata ttcagtaaaa cgctatgtcg gagacagtaa agcattacat      900

gaaacaattg ccacccacca aacaaatgtt ggtgctcgtg aagaaaaaat tgaatatcgt      960

cgtgaagatg atcgttttca tactggttat actgtgacgg actcactcaa atcagttgaa     1020

gagatcattg gttcacaatt taatgatatt ttcaaaggaa gccaatttga tgatgtgttc     1080

catggtggta atggtgtaga cactattgat ggtaacgatg gtgacgatca tttatttggt     1140

ggcgcaggcg atgatgttat cgatggagga aacggtaaca atttccttgt tggaggaacc     1200

ggtaatgata ttatctcggg aggtaaagat aatgatattt atgtccataa aacaggcgat     1260

ggaaatgatt ctattacaga ctctggcgga caagataaac tggcattttc ggatgtaaat     1320

cttaaagacc tcacctttaa gaaagtagat tcttctctcg aaatcattaa tcaaaaagga     1380

gaaaaagttc gtattgggaa ttggttctta gaagatgatt tggctagcac agttgctaac     1440

tataaagcta cgaatgaccg aaaaattgag gacaagcggt ttg                       1483


<210>  39
<211>  3988
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta_apxIIIA_dfrA14sacB

<400>  39
attgggtacc gagctcgcgg ccgcgcaggt gcattagttg gcgcaccaat tactttgttg       60

gttactggta tcacaggatt aatttctggt attttagagt tctctaaaca accaatgtta      120

gatcatgttg catcgaaaat tggtaacaaa attgacgaat gggagaaaaa atacggtaaa      180

aattacttcg agaatggcta tgatgctcgt cataaagctt tcttagaaga ttcattctca      240

ttattgtcta gttttaataa acaatatgaa actgaaagag ctgttttaat tacacaacaa      300

cgttgggatg aatatattgg cgaacttgcg ggtattactg gcaaaggtga caaactctct      360

agtggtaagg cgtatgtaga ttactttcaa gaaggtaaat tattagagaa aaaacctgat      420

gactttagca aagtagtttt cgatccaact aagggcgaaa ttgatatttc aaatagccaa      480

acgtcaacgt tgttaaaatt tgttacgcca ttattaacac caggtgttaa tgccgtctga      540

agtgcgaagc ggcatcagag cagattgtac tgagagtgca ccatatggtc gacctcgagt      600

taattaacgt atgcggccgc tttagactat ttaaataata ttatttaaat tctttactat      660

agtgtacaat acacacagtc cattaaccaa aataaaagga ggaattagga tgagaacctt      720

gaaagtatca ttgatagctg cgaaagcgaa aaacggcgtg attggttgcg gtccagacat      780

accctggtcc gcgaaagggg agcagctact ttttaaagca ttgacctaca atcagtggct      840

tctggtgggt cgcaagacgt ttgaatctat gggcgcactc cccaatagga aatacgcggt      900

cgttacccgc tcaggttgga catcaaatga tgacaatgta gttgtatttc agtcaatcga      960

agaggccatg gacaggctag ctgaattcac cggtcacgtt atagtgtctg gtggcggaga     1020

aatttaccga gaaacattac ccatggcctc tacgctccac ttatcgacga tcgacatcga     1080

gccagagggg gatgttttct tcccgagtat tccaaatacc ttcgaagttg tttttgagca     1140

acactttact tcaaacatta actattgcta tcaaatttgg aaaaagggtt aatgccgtct     1200

gaagtgcggt acaagcggta gaacctgccc cgttagttga aaccgcttgt tatgcatgca     1260

tgggatccgc gaatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccattggg     1320

atccgctttt acagcgattg cagaatgatt gaattgtaaa ctttagagct ttatattttg     1380

tttaatggta ttatatttac ttatatttat gattcttagt ttttattgta aattaaagtg     1440

tttatttatt gtattttaag tataagatcc tttttaaccc atcacatata cctgccgttc     1500

actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac     1560

tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt     1620

ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga     1680

ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat     1740

cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta     1800

tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc     1860

catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga     1920

catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg     1980

cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata     2040

aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc     2100

aaaaaaatga aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt     2160

ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg     2220

caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg     2280

acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa     2340

acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag     2400

accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat     2460

tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta     2520

acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct     2580

ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca     2640

gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat     2700

acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat     2760

ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc     2820

tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc     2880

taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa     2940

cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg     3000

actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg     3060

gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt     3120

taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc     3180

aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag     3240

acaaacaatc aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg     3300

ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa     3360

gaaaatgccg atatcctatt ggcattttct tttatttctt atcaacataa aggtgaatcc     3420

catacctaga gctgcacgcg agacatgaac gtgcaactgc ttctcaggta atagtaacct     3480

aaaagcacat gatgaattac attcagtaga agaaattatt ggaagtaatc agagagacga     3540

atttaaaggt agtaaattca gagatatttt ccatggtgcc gatggtgatg atctattaaa     3600

tggtaatgat ggggatgata ttctatacgg tgataaaggt aacgatgagt taagaggtga     3660

taatggtaac gaccaacttt atggtggtga aggtaatgac aaactattag gaggtaatgg     3720

caataattac ctcagtggtg gtgatggcaa tgatgagctt caagtcttag gcaatggttt     3780

taatgtgctt cgtggcggta aaggcgatga taaactttat ggtagctcag gttctgattt     3840

acttgatggt ggagaaggta atgattatct agaaggaggc gatggtagcg atttttatgt     3900

ttatcgttcc acttcaggta atcatactat ttatgatcaa ggtaaatcta gtgatttaga     3960

taaagatccg aattcctgtg tgaaatgg                                        3988


<210>  40
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIIIAmut

<400>  40
gaccgcggcc gcgcaggtgc attagttggc gcaccaatta ctttgttggt tactggtatc       60

acaggattaa tttctggtat tttagagttc tctaaacaac caatgttaga tcatgttgca      120

tcgaaaattg gtaacaaaat tgacgaatgg gagaaaaaat acggtaaaaa ttacttcgag      180

aatggctatg atgctcgtca taaagctttc ttagaagatt cattctcatt attgtctagt      240

tttaataaac aatatgaaac tgaaagagct gttttaatta cacaacaacg ttgggatgaa      300

tatattggcg aacttgcggg tattactggc aaaggtgaca aactctctag tggtaaggcg      360

tatgtagatt actttcaaga aggtaaatta ttagagaaaa aacctgatga ctttagcaaa      420

gtagttttcg atccaactaa gggcgaaatt gatatttcaa atagccaaac gtcaacgttg      480

ttaaaatttg ttacgccatt attaacacca ggtacagagt cacgtgaaag aactcaaaca      540

ggtgcatatg aatatatcac gaagttagtt gtaaaaggta aagataaatg ggttgttaat      600

ggcgttaaag ataaaggtgc cgtttatgat tatactaatt taattcaaca tgctcatatt      660

agttcatcag tagcacgtgg tgaagaatac cgtgaagttc gtttggtatc tcatctaggc      720

aatggtaatg acaaagtgtt cttagctgcg ggttccgcag aaattcacgc tggtgaaggt      780

catgatgtgg tttattatga taaaaccgat acaggtcttt tagtaattga tggaaccaaa      840

gcgactgaac aagggcgtta ttctgttacg cgcgaattga gtggtgctac aaaaatcctg      900

agagaagtaa taaaaaatca aaaatctgct gttggtgcac gtgaagaaac cttggaatat      960

cgtgattatg aattaacgca atcaggtaat agtaacctaa aagcacatga tgaattacat     1020

tcagtagaag aaattattgg aagtaatcag agagacgaat ttaaaggtag taaattcaga     1080

gatattttcc atggtgccga tggtgatgat ctattaaatg gtaatgatgg ggatgatatt     1140

ctatacggtg ataaaggtaa cgatgagtta agaggtgata atggtaacga ccaactttat     1200

ggtggtgaag gtaatgacaa actattagga ggtaatggca ataattacct cagtggtggt     1260

gatggcaatg atgagcttca agtcttaggc aatggtttta atgtgcttcg tggcggtaaa     1320

ggcgatgata aactttatgg tagctcaggt tctgatttac ttgatggtgg agaaggtaat     1380

gattatctag aaggaggcga tggtagcgat ttttatgttt atcgttccac ttcaggtaat     1440

catactattt atgatcaagg taaatctagt gatttagata caagcggttt g              1491


<210>  41
<211>  4050
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta_apxIA_trunc_dfrA14sacB

<400>  41
attgggtacc gagctcgcgg ccgcctaatt cacccgcttg cgattgcggg tctaaagtac       60

cgccgtacca aacgtccgct tgcggattat ttttttccgc ttcgattttt gcaaaggtac      120

tgccggaacc gttgcggata aaagaggttt tcacatcata tttttgttcg aatgtttttg      180

ccgcattctc acacatcaca ttggtcgcac tacagtaaat cactaaacgt ccttttgcct      240

gagccgccga actgaacatt aagcccgcac caagtaatgc ggttgaaacc gctaaagaaa      300

gttttccaaa tttcataatc aaagcctcat attgagcata aatcaataaa atgccgcgaa      360

tataatcgaa agcatttttc ttattggaac taatttaccg taattgaata aaaaataccg      420

tgaagcagtt cacaaaatac gagattaatg agcgatattg ttataaaatc ataatgtaaa      480

cctcatttgt aatgaattgg taaattatat aaataatcaa aaaacttact tttttttatt      540

tttatcggta agtatttaca atcaagtcag acaaacagta agattgaagg ttaatgccgt      600

ctgaagtgcg aagcggcatc agagcagatt gtactgagag tgcaccatat ggtcgacctc      660

gagttaatta acgtatgcgg ccgctttaga ctatttaaat aatattattt aaattcttta      720

ctatagtgta caatacacac agtccattaa ccaaaataaa aggaggaatt aggatgagaa      780

ccttgaaagt atcattgata gctgcgaaag cgaaaaacgg cgtgattggt tgcggtccag      840

acataccctg gtccgcgaaa ggggagcagc tactttttaa agcattgacc tacaatcagt      900

ggcttctggt gggtcgcaag acgtttgaat ctatgggcgc actccccaat aggaaatacg      960

cggtcgttac ccgctcaggt tggacatcaa atgatgacaa tgtagttgta tttcagtcaa     1020

tcgaagaggc catggacagg ctagctgaat tcaccggtca cgttatagtg tctggtggcg     1080

gagaaattta ccgagaaaca ttacccatgg cctctacgct ccacttatcg acgatcgaca     1140

tcgagccaga gggggatgtt ttcttcccga gtattccaaa taccttcgaa gttgtttttg     1200

agcaacactt tacttcaaac attaactatt gctatcaaat ttggaaaaag ggttaatgcc     1260

gtctgaagtg cggtacaagc ggtagaacct gccccgttag ttgaaaccgc ttgttatgca     1320

tgcatgggat ccgcgaatcc cgcggccatg gcggccggga gcatgcgacg tcgggcccat     1380

tgggatccgc ttttacagcg attgcagaat gattgaattg taaactttag agctttatat     1440

tttgtttaat ggtattatat ttacttatat ttatgattct tagtttttat tgtaaattaa     1500

agtgtttatt tattgtattt taagtataag atccttttta acccatcaca tatacctgcc     1560

gttcactatt atttagtgaa atgagatatt atgatatttt ctgaattgtg attaaaaagg     1620

caactttatg cccatgcaac agaaactata aaaaatacag agaatgaaaa gaaacagata     1680

gattttttag ttctttaggc ccgtagtctg caaatccttt tatgattttc tatcaaacaa     1740

aagaggaaaa tagaccagtt gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt     1800

aaatcgcgcg ggtttgttac tgataaagca ggcaagacct aaaatgtgta aagggcaaag     1860

tgtatacttt ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg cgtaactaac     1920

ttgccatctt caaacaggag ggctggaaga agcagaccgc taacacagta cataaaaaag     1980

gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact     2040

accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca     2100

tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa     2160

cagcaaaaaa atgaaaaata tcaagttcct gaattcgatt cgtccacaat taaaaatatc     2220

tcttctgcaa aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact     2280

gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg     2340

gatgacacat cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg     2400

aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta     2460

aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt     2520

ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa     2580

gttaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca     2640

atctttgacg gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac     2700

tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac     2760

aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct     2820

ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa     2880

cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt     2940

gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca     3000

gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc     3060

actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg     3120

cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt     3180

gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta     3240

cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac     3300

gcagacaaac aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca     3360

tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataaaaacgc     3420

aaaagaaaat gccgatatcc tattggcatt ttcttttatt tcttatcaac ataaaggtga     3480

atcccatacc tagagctgca cgcgagacat gaacgtgcaa ctgcttcgat agttattttt     3540

agatgataaa tagcaatcct atatatatta ggtgtgtagg attgctattt tatttatgga     3600

ggagcaaatg gatttttatc gggaagaaga ctacggatta tacgcactga cgattttagc     3660

ccagtaccat aatattgctg taaatccgga agaactaaaa cataaattcg accttgaagg     3720

aaaaggctta gatctaaccg cttggctatt agccgcaaaa tcattagaac ttaaagcaaa     3780

acaagtaaaa aaagcgattg atcgtttggc gtttatcgca ctaccggcac ttgtatggcg     3840

agaagacggt aaacatttta ttttgactaa aattgataat gaagcaaaaa aatatttaat     3900

ttttgatttg gaaacgcata atcctcgcat tttggaacaa gcggaattcg agagcttata     3960

ccaaggaaaa ctgattttag ttgcatcaag agcttccatc gtaggtaagc tggcaaagtt     4020

tgacttgatc cgaattcctg tgtgaaatgg                                      4050


<210>  42
<211>  4756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIAmut_long

<400>  42
aaacaagcgg tccggatctt ggaatttcgg cataatttga tcgatattcg gcgaacgata       60

cgcttctaat aagcctaatt cacccgcttg cgattgcggg tctaaagtac cgccgtacca      120

aacgtccgct tgcggattat ttttttccgc ttcgattttt gcaaaggtac tgccggaacc      180

gtttcggata aaagaggttt tcacatcata tttttgttcg aatgtttttg ccgcattctc      240

acacatcaca ttggtcgcac tacagtaaat cactaaacgt ccttttgcct gagccgccga      300

actgaacatt aagcccgcac caagtaatgc ggttgaaacc gctaaagaaa gttttccaaa      360

tttcataatc aaagcctcat attgagcata aatcaacaaa atgccgcgaa tataatcgaa      420

agcatttttc ttattggaac taatttaccg taattgaata aaaaataccg tgaagcagtt      480

cacaaaatac gagattaatg agcgatattg ttataaaatc ataatgtaaa cctcatttgt      540

aatgaattgg taaattatat aaataatcaa aaaacttact tttttttatt tttatcggta      600

agtatttaca atcaagtcag acaaacggca atattgttat aaatctgggg ggatgaatga      660

gtaaaaaaat taatggattt gaggttttag gagaggtggc atggttatgg gcaagttctc      720

ctttacatcg aaagtggccg ctttctttgt tagcaattaa tgtgctacct gcgattgaga      780

gtaatcaata tgttttgtta aagcgtgacg gttttcctat tgcattttgt agctgggcaa      840

atttgaattt ggaaaatgaa attaaatacc ttgatgatgt tgcctcgcta gttgcggatg      900

attggacttc cggcgatcgt cgatggttta tagattggat agcaccgttc ggagacagtg      960

ccgcattata caaacatatg cgagataact tcccgaatga gctgtttagg gctattcgag     1020

ttgatccgga ctctcgagta gggaaaattt cagaatttca tggaggaaaa attgataaga     1080

aactggcaag taaaattttt caacaatatc actttgaatt aatgagtgag ctaaaaaata     1140

aacaaaattt taaattttca ttagtaaata gctaaggaga caacatggct aactctcagc     1200

tcgatagagt caaaggattg attgattcac ttaatcaaca tacaaaaagt gcagctaaat     1260

caggtgccgg cgcattaaaa aatggtttgg gacaggtgaa gcaagcaggg cagaaattaa     1320

ttttatatat tccgaaagat tatcaagcta gtaccggctc aagtcttaat gatttagtga     1380

aagcggcgga ggctttaggg atcgaagtac atcgctcgga aaaaaacggt accgcactag     1440

cgaaagaatt attcggtaca acggaaaaac tattaggttt ctcggaacga ggcatcgcat     1500

tatttgcacc tcagtttgat aagttactga ataagaacca aaaattaagt aaatcgctcg     1560

gcggttcatc ggaagcatta ggacaacgtt taaataaaac gcaaacggca ctttcagcct     1620

tacaaagttt cttaggtacg gctattgcgg gtatggatct tgatagcctg cttcgtcgcc     1680

gtagaaacgg tgaggacgtc agtggttcgg aattagctaa agcaggtgtg gatctagccg     1740

ctcagttagt ggataacatt gcaagtgcaa cgggtacggt ggatgcgttt gccgaacaat     1800

taggtaaatt gggcaatgcc ttatctaaca ctcgcttaag cggtttagca agtaagttaa     1860

ataaccttcc agatttaagc cttgcaggac ctgggtttga tgccgtatca ggtatcttat     1920

ctgttgtttc ggcttcattc attttaagta ataaagatgc cgatgcaggt acaaaagcgg     1980

cggcaggtat tgaaatctca actaaaatct taggcaatat cggtaaagcg gtttctcaat     2040

atattattgc gcaacgtgtg gcggcaggct tatccacaac tgcggcaacc ggtggtttaa     2100

tcggttcggt cgtagcatta gcgattagcc cgctttcgtt cttaaatgtt gcggataagt     2160

ttgaacgtgc gaaacagctt gaacaatatt cggagcgctt taaaaagttc ggttatgaag     2220

gtgatagttt attagcttca ttctaccgtg aaaccggtgc gattgaagcg gcattaacca     2280

cgattaacag tgtgttaagt gcggcttccg caggtgttgg ggctgctgca accggctcat     2340

tagtcggtgc gccggtagca gctttagtta gtgcaatcac cggtattatt tcaggtattt     2400

tagatgcttc taaacaggca atcttcgaac gagttgcaac gaaattagcg aataagattg     2460

acgaatggga gaaaaaacac ggtaaaaact attttgaaaa cggttatgac gcccgccatt     2520

ccgcattctt agaagatacc tttgaattgt tatcacaata caataaagag tattcggtag     2580

agcgtgtcgt tgctattacg caacagcgtt gggatgtcaa tatcggtgaa cttgccggca     2640

ttactcgcaa aggttctgat acgaaaagcg gtaaagctta cgttgatttc tttgaagaag     2700

gaaaactttt agagaaagaa ccggatcgtt ttgataaaaa agtgtttgat ccgcttgaag     2760

gcaaaatcga cctttcttca attaacaaaa ccactttatt gaaatttatt acaccggttt     2820

ttaccgcagg tgaagagatt cgtgagcgta agcaaaccgg tgcatacgaa tatatgaccg     2880

aattattcgt taaaggtaaa gaaaaatggg tggtaaccgg tgtgcagtca cataatgcga     2940

tttatgacta tacgaatctt atccaattag cgatagataa aaaaggtgaa aaacgtcaag     3000

tgaccattga atctcatttg ggtgagaaaa atgatcgtat atatctttca tccggttcat     3060

ctatcgtata tgcgggtaac ggacatgatg tagcatatta cgataaaacc gatacaggtt     3120

acttaacatt tgacggacaa agtgcacaga aagccggtga atatattgtc actaaagaac     3180

ttaaagctga tgtaaaagtt ttaaaagaag tggttaaaac tcaggatatt tcagttggag     3240

cacgcagtga aaaattagaa tatcgtgatt atgagttaag cccattcgaa cttgggaacg     3300

gtatcagagc taaagatgaa ttacattctg ttgaagaaat tatcggtagt aatcgtaaag     3360

acaaattctt tggtagtcgc tttaccgata ttttccatgg tgcgaaaggc gatgatgaaa     3420

tctacggtaa tgacggccac gatatcttat acggagacga cggtaatgat gtaatccatg     3480

gcggtgacgg taacgaccat cttgttggtg gtaacggaaa cgaccgatta atcggcggaa     3540

aaggtaataa tttccttaat ggcggtgatg gtgacgatga gttgcaggtc tttgagggtc     3600

aatacaacgt attattaggt ggtgcgggta atgacattct gtatggcagc gatggtacta     3660

acttatttga cggtggtgta ggcaatgaca aaatctacgg tggtttaggt aaggatattt     3720

atcgctacag taaggagtac ggtcgtcata tcattattga gaaaggcggt gatgatgata     3780

cgttattgtt atcggatctt agttttaaag atgtaggatt tatcagaatc ggtgatgatc     3840

ttcttgtgaa taaaagaatc ggaggaacac tgtattacca tgaagattac aatgggaatg     3900

cgctcacgat taaagattgg ttcaaggaag gtaaagaagg acaaaataat aaaattgaaa     3960

aaatcgttga taaagatgga gcttatgttt taagccaata tctgactgaa ctgacagctc     4020

ctggaagagg tatcaattac tttaatgggt tagaagaaaa attgtattat ggagaaggat     4080

ataatgcact tcctcaactc agaaaagata ttgaacaaat catttcatct acgggtgcat     4140

ttaccggtga tcacggaaaa gtatctgtag gctcaggcgg accgttagtc tataataact     4200

cagctaacaa tgtagcaaat tctttgagtt attctttagc acaagcagct taagatagtt     4260

atttttagat gataaatagc aatcctatat atattaggtg tgtaggattg ctattttatt     4320

tatggaggag caaatggatt tttatcggga agaagactac ggattatacg cactgacgat     4380

tttagcccag taccataata ttgctgtaaa tccggaagaa ctaaaacata aattcgacct     4440

tgaaggaaaa ggcttagatc taaccgcttg gctattagcc gcaaaatcat tagaacttaa     4500

agcaaaacaa gtaaaaaaag cgattgatcg tttggcgttt atcgcactac cggcacttgt     4560

atggcgagaa gacggtaaac attttatttt gactaaaatt gataatgaag caaaaaaata     4620

tttaattttt gatttggaaa cgcataatcc tcgcattttg gaacaagcgg aattcgagag     4680

cttataccaa ggaaaactga ttttagttgc atcaagagct tccatcgtag gtaagctggc     4740

aaagtaccgc ttgtaa                                                     4756


<210>  43
<211>  3981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta_apxIVA_ dfrA14sacB

<400>  43
atccacaagc ggtcatctgg cgcgaataga gaacctgaac aatgggaaaa ttacatagta       60

tttgataatt gcagtggaat taaagaaaga caccaactgt attaaaaata gattagaagg      120

agacaacacg atgacaaaac taactatgca agatgtgact aatttatatt tatataagca      180

aagaacttta cctacggata ggttagatga ttcgcttatt agcaaaacag gaaaagggga      240

aaatattgat aaaaaggaat ttatggcggg gccgggacgt tttgtgacgg ccgataattt      300

tagtgttgta aaagactttt ttactgcaaa ggattcatta ataaacctaa gcttgcagac      360

tcgtatatta gcgaatttaa agccgggcaa atattccaaa gcgcagatat tagaaatgtt      420

gggctatacg aaaaatggag aaaaggtaga tggcatgttt accggtgaag tccagacatt      480

aggcttttat gacgatggca aaggggattt actcgaacgc gttaatgccg tctgaagtgc      540

gaagcggcat cagagcagat tgtactgaga gtgcaccata tggtcgacct cgagttaatt      600

aacgtatgcg gccgctttag actatttaaa taatattatt taaattcttt actatagtgt      660

acaatacaca cagtccatta accaaaataa aaggaggaat taggatgaga accttgaaag      720

tatcattgat agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct      780

ggtccgcgaa aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg      840

tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta      900

cccgctcagg ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg      960

ccatggacag gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt     1020

accgagaaac attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag     1080

agggggatgt tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact     1140

ttacttcaaa cattaactat tgctatcaaa tttggaaaaa gggttaatgc cgtctgaagt     1200

gcggtacaag cggtagaacc tgccccgtta gttgaaaccg cttgttatgc atgcatggga     1260

tccgcgaatc ccgcggccat ggcggccggg agcatgcgac gtcgggccca ttgggatccg     1320

cttttacagc gattgcagaa tgattgaatt gtaaacttta gagctttata ttttgtttaa     1380

tggtattata tttacttata tttatgattc ttagttttta ttgtaaatta aagtgtttat     1440

ttattgtatt ttaagtataa gatccttttt aacccatcac atatacctgc cgttcactat     1500

tatttagtga aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat     1560

gcccatgcaa cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta     1620

gttctttagg cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa     1680

atagaccagt tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc     1740

gggtttgtta ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt     1800

tggcgtcacc ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct     1860

tcaaacagga gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga     1920

acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg     1980

ctggcaggag gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa     2040

acatacggca tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa     2100

aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat ctcttctgca     2160

aaaggcctgg acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac     2220

tatcacggct accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca     2280

tcgatttaca tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct     2340

ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa     2400

acacaagaat ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac     2460

actgatttct ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta     2520

tcagcatcag acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac     2580

ggtgacggaa aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca     2640

ggcgacaacc atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta     2700

gtatttgaag caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac     2760

aaagcatact atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa     2820

agcgataaaa aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac     2880

gatgattaca cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat     2940

gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc     3000

cgcggatcaa aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat     3060

gtttctaatt ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa     3120

atggatcttg atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg     3180

aaaggaaaca atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa     3240

caatcaacgt ttgcgccaag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc     3300

aaagacagca tccttgaaca aggacaatta acagttaaca aataaaaacg caaaagaaaa     3360

tgccgatatc ctattggcat tttcttttat ttcttatcaa cataaaggtg aatcccatac     3420

ctagagctgc acgcgagaca tgaacgtgca actgcttcca attcgaaggg aaatgggtaa     3480

ccgattattc tcgtactgaa gccttattta actctacttt taaacaatcg cctgaaaatg     3540

cattatatga tttaagcgaa tacctttctt tctttaacga tcctacggaa tggaaagaag     3600

ggctattact gttaagccgt tatatagatt atgctaaagc acaaggattt tatgaaaact     3660

gggcggctac ttctaactta actattgccc gtttaagaga ggctggagta atttttgcag     3720

aatcgacgga tttaaaaggc gatgaaaaaa ataatatttt gttaggtagc caaaaagata     3780

ataacttatc gggtagtgca ggtgatgatc tacttatcgg cggagagggt aatgatacgt     3840

taaaaggcag ctacggtgcg gacacctata tctttagcaa aggacacgga caggatatcg     3900

tttatgaaga taccaataat gataaccgag caagagatat cgacacctta aaatttggat     3960

ccgaattcct gtgtgaaatg g                                               3981


<210>  44
<211>  1043
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  apxIV_int_del

<400>  44
atccacaagc ggtcatctgg cgcgaataga gaacctgaac aatgggaaaa ttacatagta       60

tttgataatt gcagtggaat taaagaaaga caccaactgt attaaaaata gattagaagg      120

agacaacacg atgacaaaac taactatgca agatgtgact aatttatatt tatataagca      180

aagaacttta cctacggata ggttagatga ttcgcttatt agcaaaacag gaaaagggga      240

aaatattgat aaaaaggaat ttatggcggg gccgggacgt tttgtgacgg ccgataattt      300

tagtgttgta aaagactttt ttactgcaaa ggattcatta ataaacctaa gcttgcagac      360

tcgtatatta gcgaatttaa agccgggcaa atattccaaa gcgcagatat tagaaatgtt      420

gggctatacg aaaaatggag aaaaggtaga tggcatgttt accggtgaag tccagacatt      480

aggcttttat gacgatggca aaggggattt actcgaacgc caattcgaag ggaaatgggt      540

aaccgattat tctcgtactg aagccttatt taactctact tttaaacaat cgcctgaaaa      600

tgcattatat gatttaagcg aatacctttc tttctttaac gatcctacgg aatggaaaga      660

agggctatta ctgttaagcc gttatataga ttatgctaaa gcacaaggat tttatgaaaa      720

ctgggcggct acttctaact taactattgc ccgtttaaga gaggctggag taatttttgc      780

agaatcgacg gatttaaaag gcgatgaaaa aaataatatt ttgttaggta gccaaaaaga      840

taataactta tcgggtagtg caggtgatga tctacttatc ggcggagagg gtaatgatac      900

gttaaaaggc agctacggtg cggacaccta tatctttagc aaaggacacg gacaggatat      960

cgtttatgaa gataccaata atgataaccg agcaagagat atcgacacct taaaatttgg     1020

atccgaattc ctgtgtgaaa tgg                                             1043


<210>  45
<211>  4390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sxy_dfrA14sacB_insert

<400>  45
gtaccgcttg ttaaatgatt acaccaagcg actctaaaaa tcttcgtatc tatatcataa       60

atacgatgag cgttaaagtg gcgatattct agtgtaaaaa acacttaaaa gcaagattta      120

attttatttt tctaaaaaat atagtttcaa acgaatcgga catattttta ccctttatta      180

tatttacatt attgacatta aataatttat tttgcaaaat atacataaat ttcgctcatt      240

aaaaaataat catatataaa aaaggagaaa cataatggca atatccccaa aaaagttcca      300

atatcttaag gagattttta gtcctcttgg agaaattaac ttcaaaagct atttttctta      360

cttaggaata tttaaagacg atactatgtt cgccctctat gatcataaaa acgatcgatt      420

atacttaaga aaatccgctc aattttatcc ggatattata agaacaatac cgatacattt      480

tttaattgat cgtcgtatcg gtaagcaaca atctcatatt ttttatctta taccttcttc      540

tattattcac aatcttcatt tatatactca ttggattctc tctgctatcg aagaatatca      600

aactgcaaag gccaaattga tttctcaaaa taaaaataaa attcgtctgc ttcccaattt      660

gaatatcaat atagaaagat tattggcacg tattgagatt tataccgtag atgatttaaa      720

aaacgtaggc gtgattaatg cgtttgtaaa actgataatg ctaggcttgg aagtaaccga      780

attactcctc ttcaaactct acgctgcgct cgaacataaa tatatctata tgttatccaa      840

gcaagaaaaa caatccctat taattgaagc cgatttatct ctctataacg caggcctacg      900

taaacgcttc gctatctcac aggctaacta agttaatgcc gtctgaagtg cgaagcggca      960

tcagagcaga ttgtactgag agtgcaccat atggtcgacc tcgagttaat taacgtatgc     1020

ggccgcttta gactatttaa ataatattat ttaaattctt tactatagtg tacaatacac     1080

acagtccatt aaccaaaata aaaggaggaa ttaggatgag aaccttgaaa gtatcattga     1140

tagctgcgaa agcgaaaaac ggcgtgattg gttgcggtcc agacataccc tggtccgcga     1200

aaggggagca gctacttttt aaagcattga cctacaatca gtggcttctg gtgggtcgca     1260

agacgtttga atctatgggc gcactcccca ataggaaata cgcggtcgtt acccgctcag     1320

gttggacatc aaatgatgac aatgtagttg tatttcagtc aatcgaagag gccatggaca     1380

ggctagctga attcaccggt cacgttatag tgtctggtgg cggagaaatt taccgagaaa     1440

cattacccat ggcctctacg ctccacttat cgacgatcga catcgagcca gagggggatg     1500

ttttcttccc gagtattcca aataccttcg aagttgtttt tgagcaacac tttacttcaa     1560

acattaacta ttgctatcaa atttggaaaa agggttaatg ccgtctgaag tgcggtacaa     1620

gcggtagaac ctgccccgtt agttgaaacc gcttgttatg catgcatggg atccgcgaat     1680

cccgcggcca tggcggccgg gagcatgcga cgtcgggccc attgggatcc gcttttacag     1740

cgattgcaga atgattgaat tgtaaacttt agagctttat attttgttta atggtattat     1800

atttacttat atttatgatt cttagttttt attgtaaatt aaagtgttta tttattgtat     1860

tttaagtata agatcctttt taacccatca catatacctg ccgttcacta ttatttagtg     1920

aaatgagata ttatgatatt ttctgaattg tgattaaaaa ggcaacttta tgcccatgca     1980

acagaaacta taaaaaatac agagaatgaa aagaaacaga tagatttttt agttctttag     2040

gcccgtagtc tgcaaatcct tttatgattt tctatcaaac aaaagaggaa aatagaccag     2100

ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa gtaaatcgcg cgggtttgtt     2160

actgataaag caggcaagac ctaaaatgtg taaagggcaa agtgtatact ttggcgtcac     2220

cccttacata ttttaggtct ttttttattg tgcgtaacta acttgccatc ttcaaacagg     2280

agggctggaa gaagcagacc gctaacacag tacataaaaa aggagacatg aacgatgaac     2340

atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta ctaccgcact gctggcagga     2400

ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc catataagga aacatacggc     2460

atttcccata ttacacgcca tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa     2520

tatcaagttc ctgaattcga ttcgtccaca attaaaaata tctcttctgc aaaaggcctg     2580

gacgtttggg acagctggcc attacaaaac gctgacggca ctgtcgcaaa ctatcacggc     2640

taccacatcg tctttgcatt agccggagat cctaaaaatg cggatgacac atcgatttac     2700

atgttctatc aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc tggccgcgtc     2760

tttaaagaca gcgacaaatt cgatgcaaat gattctatcc taaaagacca aacacaagaa     2820

tggtcaggtt cagccacatt tacatctgac ggaaaaatcc gtttattcta cactgatttc     2880

tccggtaaac attacggcaa acaaacactg acaactgcac aagttaacgt atcagcatca     2940

gacagctctt tgaacatcaa cggtgtagag gattataaat caatctttga cggtgacgga     3000

aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca actacagctc aggcgacaac     3060

catacgctga gagatcctca ctacgtagaa gataaaggcc acaaatactt agtatttgaa     3120

gcaaacactg gaactgaaga tggctaccaa ggcgaagaat ctttatttaa caaagcatac     3180

tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa     3240

aaacgcacgg ctgagttagc aaacggcgct ctcggtatga ttgagctaaa cgatgattac     3300

acactgaaaa aagtgatgaa accgctgatt gcatctaaca cagtaacaga tgaaattgaa     3360

cgcgcgaacg tctttaaaat gaacggcaaa tggtacctgt tcactgactc ccgcggatca     3420

aaaatgacga ttgacggcat tacgtctaac gatatttaca tgcttggtta tgtttctaat     3480

tctttaactg gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa aatggatctt     3540

gatcctaacg atgtaacctt tacttactca cacttcgctg tacctcaagc gaaaggaaac     3600

aatgtcgtga ttacaagcta tatgacaaac agaggattct acgcagacaa acaatcaacg     3660

tttgcgccaa gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt caaagacagc     3720

atccttgaac aaggacaatt aacagttaac aaataaaaac gcaaaagaaa atgccgatat     3780

cctattggca ttttctttta tttcttatca acataaaggt gaatcccata cctagagctg     3840

cacgcgagac atgaacgtgc aactgcttcg taagccggtt cctttctgat tatctcaatg     3900

ctaccatcct acctataact tgttagttta tttaagtgaa atctactttt atccatagga     3960

gaacacaatg gaatttcgta ttgaaaaaga taccatgggc gaagttcaag tacctgccaa     4020

tcgttattgg gcggcacaaa cagagcgttc acgcaataat tttaaaatcg gtcccgaagc     4080

gtcaatgcct aaagaaatta ttgaagcgtt cggttacttg aaaaaagcag cggcatttgc     4140

caacacagat ttaggcgtat tacctgcgga aaaacgtgat ttaatcgctc aagcctgtga     4200

tgaaatcctt gccggtaaat taaacgaaga attcccgctt gtaatctggc aaaccggttc     4260

cggtacgcaa tccaatatga acttaaacga agttattgca aaccgtgcgc atgttattca     4320

cggcggtaaa ttaggtgaaa aatcggtaat tcacccgaat gatgaggatc cgaattcctg     4380

tgtgaaatgg                                                            4390


<210>  46
<211>  1037
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sxy_del

<400>  46
atccacaagc ggtcatctgg ctcacgtgtg gagaaatcaa tacagtaaaa cgttctttac       60

gagttggtaa aggaattgga ccacgaactt gtgcaccagt acgtttagct gtttctacga      120

tctccgcagt agattgatca attaaacgat gatcaaatgc ttttaagcgg atacggattc      180

tttggttctg cattagacca gagctccaat taaaatttag ctaataaaaa aaccgaacta      240

ccacttaagc cacatagcat aagggagcgc agttatacct atatagtttc caaatcggaa      300

acattgtatg tactacaata tctgtagtac cgcttgataa atgattacac caagcgactc      360

taaaaatctt cgtatctata tcataaatac gatgagcgtt aaagtggcga tattctagtg      420

taaaaaacac ttaaaagcaa gatttaattt tatttttcta aaaaatatag tttcaaacga      480

atcggacata tttttaccct ttattatatt tacattgtaa gccggttcct ttctgattat      540

ctcaatgcta ccatcctacc tataacttgt tagtttattt aagtgaaatc tacttttatc      600

cataggagaa cacaatggaa tttcgtattg aaaaagatac catgggcgaa gttcaagtac      660

ctgccaatcg ttattgggcg gcacaaacag agcgttcacg caataatttt aaaatcggtc      720

ccgaagcgtc aatgcctaaa gaaattattg aagcgttcgg ttacttgaaa aaagcagcgg      780

catttgccaa cacagattta ggcgtattac ctgcggaaaa acgtgattta atcgctcaag      840

cctgtgatga aatccttgcc ggtaaattaa acgaagaatt cccgcttgta atctggcaaa      900

ccggttccgg tacgcaatcc aatatgaact taaacgaagt tattgcaaac cgtgcgcatg      960

ttattcacgg cggtaaatta ggtgaaaaat cggtaattca cccgaatgat gaggatccga     1020

attcctgtgt gaaatgg                                                    1037


<210>  47
<211>  1943
<212>  PRT
<213>  Actinobacillus pleuropneumoniae

<400>  47

Met Thr Lys Leu Thr Met Gln Asp Val Thr Asn Leu Tyr Leu Tyr Lys 
1               5                   10                  15      


Gln Arg Thr Leu Pro Thr Asp Arg Leu Asp Asp Ser Leu Ile Ser Lys 
            20                  25                  30          


Thr Gly Lys Gly Glu Asn Ile Asp Lys Lys Glu Phe Met Ala Gly Pro 
        35                  40                  45              


Gly Arg Phe Val Thr Ala Asp Asn Phe Ser Val Val Lys Asp Phe Phe 
    50                  55                  60                  


Thr Ala Lys Asp Ser Leu Ile Asn Leu Ser Leu Gln Thr Arg Ile Leu 
65                  70                  75                  80  


Ala Asn Leu Lys Pro Gly Lys Tyr Ser Lys Ala Gln Ile Leu Glu Met 
                85                  90                  95      


Leu Gly Tyr Thr Lys Asn Gly Glu Lys Val Asp Gly Met Phe Thr Gly 
            100                 105                 110         


Glu Val Gln Thr Leu Gly Phe Tyr Asp Asp Gly Lys Gly Asp Leu Leu 
        115                 120                 125             


Glu Arg Ala Tyr Ile Trp Asn Thr Thr Gly Phe Lys Met Ser Asp Asn 
    130                 135                 140                 


Ala Phe Phe Val Ile Glu Glu Ser Gly Lys Arg Tyr Ile Glu Asn Phe 
145                 150                 155                 160 


Gly Ile Glu Pro Leu Gly Lys Gln Glu Asp Phe Asp Phe Val Gly Gly 
                165                 170                 175     


Phe Trp Ser Asn Leu Val Asn Arg Gly Leu Glu Ser Ile Ile Asp Pro 
            180                 185                 190         


Ser Gly Ile Gly Gly Thr Val Asn Leu Asn Phe Thr Gly Glu Val Glu 
        195                 200                 205             


Thr Tyr Thr Leu Asp Glu Thr Arg Phe Lys Ala Glu Ala Ala Lys Lys 
    210                 215                 220                 


Ser His Trp Ser Leu Val Asn Ala Ala Lys Val Tyr Gly Gly Leu Asp 
225                 230                 235                 240 


Gln Ile Ile Lys Lys Leu Trp Asp Ser Gly Ser Ile Lys His Leu Tyr 
                245                 250                 255     


Gln Asp Lys Asp Thr Gly Lys Leu Lys Pro Ile Ile Tyr Gly Thr Ala 
            260                 265                 270         


Gly Asn Asp Ser Lys Ile Glu Gly Thr Lys Ile Thr Arg Arg Ile Ala 
        275                 280                 285             


Gly Lys Glu Val Thr Leu Asp Ile Ala Asn Gln Lys Ile Glu Lys Gly 
    290                 295                 300                 


Val Leu Glu Lys Leu Gly Leu Ser Val Ser Gly Ser Asp Ile Ile Lys 
305                 310                 315                 320 


Leu Leu Phe Gly Ala Leu Thr Pro Thr Leu Asn Arg Met Leu Leu Ser 
                325                 330                 335     


Gln Leu Ile Gln Ser Phe Ser Asp Ser Leu Ala Lys Leu Asp Asn Pro 
            340                 345                 350         


Leu Ala Pro Tyr Thr Lys Asn Gly Val Val Tyr Val Thr Gly Lys Gly 
        355                 360                 365             


Asn Asp Val Leu Lys Gly Thr Glu His Glu Asp Leu Phe Leu Gly Gly 
    370                 375                 380                 


Glu Gly Asn Asp Thr Tyr Tyr Ala Arg Val Gly Asp Thr Ile Glu Asp 
385                 390                 395                 400 


Ala Asp Gly Lys Gly Lys Val Tyr Phe Val Arg Glu Lys Gly Ile Pro 
                405                 410                 415     


Lys Ala Asp Pro Lys Arg Val Glu Phe Ser Lys Tyr Ile Thr Glu Glu 
            420                 425                 430         


Glu Ile Lys Glu Val Glu Lys Gly Leu Leu Thr Tyr Ala Val Leu Glu 
        435                 440                 445             


Asn Tyr Asn Trp Glu Glu Lys Thr Ala Thr Phe Ala His Ala Thr Met 
    450                 455                 460                 


Leu Asn Glu Leu Phe Thr Asp Tyr Thr Asn Tyr Arg Tyr Lys Val Lys 
465                 470                 475                 480 


Gly Leu Lys Leu Pro Ala Val Lys Lys Leu Lys Ser Pro Leu Val Glu 
                485                 490                 495     


Phe Thr Ala Asp Leu Leu Thr Val Thr Pro Ile Asp Glu Asn Gly Lys 
            500                 505                 510         


Ala Leu Ser Glu Lys Ser Ile Thr Val Lys Asn Phe Lys Asn Gly Asp 
        515                 520                 525             


Leu Gly Ile Arg Leu Leu Asp Pro Asn Ser Tyr Tyr Tyr Phe Leu Glu 
    530                 535                 540                 


Gly Gln Asp Thr Gly Phe Tyr Gly Pro Ala Phe Tyr Ile Glu Arg Lys 
545                 550                 555                 560 


Asn Gly Gly Gly Ala Lys Asn Asn Ser Ser Gly Ala Gly Asn Ser Lys 
                565                 570                 575     


Asp Trp Gly Gly Asn Gly His Gly Asn His Arg Asn Asn Ala Ser Asp 
            580                 585                 590         


Leu Asn Lys Pro Asp Gly Asn Asn Gly Asn Asn Gln Asn Asn Gly Ser 
        595                 600                 605             


Asn Gln Asp Asn His Ser Asp Val Asn Ala Pro Asn Asn Pro Gly Arg 
    610                 615                 620                 


Asn Tyr Asp Ile Tyr Asp Pro Leu Ala Leu Asp Leu Asp Gly Asp Gly 
625                 630                 635                 640 


Leu Glu Thr Val Ser Met Asn Gly Arg Gln Gly Ala Leu Phe Asp His 
                645                 650                 655     


Glu Gly Lys Gly Ile Arg Thr Ala Thr Gly Trp Leu Ala Ala Asp Asp 
            660                 665                 670         


Gly Phe Leu Val Leu Asp Arg Asn Gln Asp Gly Ile Ile Asn Asp Ile 
        675                 680                 685             


Ser Glu Leu Phe Ser Asn Lys Asn Gln Leu Ser Asp Gly Ser Ile Ser 
    690                 695                 700                 


Ala His Gly Phe Ala Thr Leu Ala Asp Leu Asp Thr Asn Gln Asp Gln 
705                 710                 715                 720 


Arg Ile Asp Gln Asn Asp Lys Leu Phe Ser Lys Leu Gln Ile Trp Arg 
                725                 730                 735     


Asp Leu Asn Gln Asn Gly Phe Ser Glu Ala Asn Glu Leu Phe Ser Leu 
            740                 745                 750         


Glu Ser Leu Asn Ile Lys Ser Leu His Thr Ala Tyr Glu Glu Arg Asn 
        755                 760                 765             


Asp Phe Leu Ala Gly Asn Asn Ile Leu Ala Gln Leu Gly Lys Tyr Glu 
    770                 775                 780                 


Lys Thr Asp Gly Thr Phe Ala Gln Met Gly Asp Leu Asn Phe Ser Phe 
785                 790                 795                 800 


Asn Pro Phe Tyr Ser Arg Phe Thr Glu Ala Leu Asn Leu Thr Glu Gln 
                805                 810                 815     


Gln Arg Arg Thr Ile Asn Leu Thr Gly Thr Gly Arg Val Arg Asp Leu 
            820                 825                 830         


Arg Glu Ala Ala Ala Leu Ser Glu Glu Leu Ala Ala Leu Leu Gln Gln 
        835                 840                 845             


Tyr Thr Lys Ala Ser Asp Phe Gln Ala Gln Arg Glu Leu Leu Pro Ala 
    850                 855                 860                 


Ile Leu Asp Lys Trp Ala Ala Thr Asp Leu Gln Tyr Gln His Tyr Asp 
865                 870                 875                 880 


Lys Thr Leu Leu Lys Thr Val Glu Ser Thr Asp Ser Ser Ala Ser Val 
                885                 890                 895     


Val Arg Val Thr Pro Ser Gln Leu Ser Ser Ile Arg Asn Ala Lys His 
            900                 905                 910         


Asp Pro Thr Val Met Gln Asn Phe Glu Gln Ser Lys Ala Lys Ile Ala 
        915                 920                 925             


Thr Leu Asn Ser Leu Tyr Gly Leu Asn Ile Asp Gln Leu Tyr Tyr Thr 
    930                 935                 940                 


Thr Asp Lys Asp Ile Arg Tyr Ile Thr Asp Lys Val Asn Asn Met Tyr 
945                 950                 955                 960 


Gln Thr Thr Val Glu Leu Ala Tyr Arg Ser Leu Leu Leu Gln Thr Arg 
                965                 970                 975     


Leu Lys Lys Tyr Val Tyr Ser Val Asn Ala Lys Gln Phe Glu Gly Lys 
            980                 985                 990         


Trp Val Thr Asp Tyr Ser Arg Thr  Glu Ala Leu Phe Asn  Ser Thr Phe 
        995                 1000                 1005             


Lys Gln  Ser Pro Glu Asn Ala  Leu Tyr Asp Leu Ser  Glu Tyr Leu 
    1010                 1015                 1020             


Ser Phe  Phe Asn Asp Pro Thr  Glu Trp Lys Glu Gly  Leu Leu Leu 
    1025                 1030                 1035             


Leu Ser  Arg Tyr Ile Asp Tyr  Ala Lys Ala Gln Gly  Phe Tyr Glu 
    1040                 1045                 1050             


Asn Trp  Ala Ala Thr Ser Asn  Leu Thr Ile Ala Arg  Leu Arg Glu 
    1055                 1060                 1065             


Ala Gly  Val Ile Phe Ala Glu  Ser Thr Asp Leu Lys  Gly Asp Glu 
    1070                 1075                 1080             


Lys Asn  Asn Ile Leu Leu Gly  Ser Gln Lys Asp Asn  Asn Leu Ser 
    1085                 1090                 1095             


Gly Ser  Ala Gly Asp Asp Leu  Leu Ile Gly Gly Glu  Gly Asn Asp 
    1100                 1105                 1110             


Thr Leu  Lys Gly Ser Tyr Gly  Ala Asp Thr Tyr Ile  Phe Ser Lys 
    1115                 1120                 1125             


Gly His  Gly Gln Asp Ile Val  Tyr Glu Asp Thr Asn  Asn Asp Asn 
    1130                 1135                 1140             


Arg Ala  Arg Asp Ile Asp Thr  Leu Lys Phe Thr Asp  Val Asn Tyr 
    1145                 1150                 1155             


Ala Glu  Val Lys Phe Arg Arg  Val Asp Asn Asp Leu  Met Leu Phe 
    1160                 1165                 1170             


Gly Tyr  His Asp Thr Asp Ser  Val Thr Val Lys Ser  Phe Tyr Asn 
    1175                 1180                 1185             


His Glu  Tyr Tyr Gln Phe Glu  Lys Leu Glu Phe Ala  Asp Arg Ser 
    1190                 1195                 1200             


Ile Thr  Arg Asp Glu Leu Gly  Lys Gln Gly Met Ala  Leu Phe Gly 
    1205                 1210                 1215             


Thr Asp  Gly Asp Asp Asp Ile  Asn Asp Trp Gly Arg  Asn Ser Val 
    1220                 1225                 1230             


Ile Asp  Ala Gly Ala Gly Asn  Asp Thr Ile Asn Gly  Ser Tyr Gly 
    1235                 1240                 1245             


Asp Asp  Thr Leu Ile Gly Gly  Thr Gly Asn Asp Ile  Leu Lys Gly 
    1250                 1255                 1260             


Ser Tyr  Gly Ala Asp Thr Tyr  Leu Phe Ser Lys Gly  His Gly Gln 
    1265                 1270                 1275             


Asp Val  Ile Tyr Glu Tyr Ser  Asp Ser Ala Asn Ser  Lys Arg Asp 
    1280                 1285                 1290             


Ile Asp  Thr Leu Lys Phe Thr  Asp Val Asn Tyr Ala  Glu Val Lys 
    1295                 1300                 1305             


Phe Arg  Arg Val Gly Asp Asp  Leu Met Leu Phe Gly  Tyr His Asp 
    1310                 1315                 1320             


Thr Asp  Ser Val Thr Val Lys  Ser Phe Tyr Asp His  Glu Tyr Tyr 
    1325                 1330                 1335             


Gln Phe  Glu Lys Leu Glu Phe  Ala Asp Arg Ser Ile  Ser Arg Asp 
    1340                 1345                 1350             


Glu Leu  Ile Lys Ala Gly Leu  His Leu Tyr Gly Thr  Asp Gly Asn 
    1355                 1360                 1365             


Asp Glu  Ile Asn Asp His Ala  Asp Trp Asp Ser Ile  Leu Glu Gly 
    1370                 1375                 1380             


Gly Lys  Gly Asn Asp Ile Leu  Arg Gly Ser Tyr Gly  Ala Asp Thr 
    1385                 1390                 1395             


Tyr Leu  Phe Ser Lys Gly His  Gly Gln Asp Val Ile  Tyr Glu Tyr 
    1400                 1405                 1410             


Ser Asp  Ser Ala Asn Ser Lys  Ser Asp Ile Asp Thr  Leu Lys Phe 
    1415                 1420                 1425             


Thr Asp  Ile Asn Tyr Ala Glu  Val Lys Phe Arg Arg  Val Gly Asp 
    1430                 1435                 1440             


Asp Leu  Met Leu Phe Gly Tyr  His Asp Thr Asp Ser  Val Thr Val 
    1445                 1450                 1455             


Lys Ser  Phe Tyr Asn His Glu  Tyr Tyr Gln Phe Glu  Lys Leu Glu 
    1460                 1465                 1470             


Phe Ala  Asp Arg Ser Ile Thr  Arg Asp Glu Leu Gly  Lys Gln Gly 
    1475                 1480                 1485             


Met Ala  Leu Phe Gly Thr Asp  Gly Asp Asp Asp Ile  Asn Asp Trp 
    1490                 1495                 1500             


Gly Arg  Asn Ser Val Ile Asp  Ala Gly Ala Gly Asn  Asp Thr Ile 
    1505                 1510                 1515             


Asn Gly  Ser Tyr Gly Asp Asp  Thr Leu Ile Gly Gly  Thr Gly Asn 
    1520                 1525                 1530             


Asp Ile  Leu Lys Gly Ser Tyr  Gly Ala Asp Thr Tyr  Leu Phe Ser 
    1535                 1540                 1545             


Lys Gly  His Gly Gln Asp Val  Ile Tyr Glu Tyr Ser  Asp Ser Ala 
    1550                 1555                 1560             


Asn Ser  Lys Arg Asp Ile Asp  Thr Leu Lys Phe Thr  Asp Val Asn 
    1565                 1570                 1575             


Tyr Ala  Glu Val Lys Phe Arg  Arg Val Gly Asp Asp  Leu Met Leu 
    1580                 1585                 1590             


Phe Gly  Tyr His Asp Thr Asp  Ser Val Thr Val Lys  Ser Phe Tyr 
    1595                 1600                 1605             


Ser His  Glu Tyr Tyr Gln Phe  Glu Lys Leu Glu Phe  Ala Asp Arg 
    1610                 1615                 1620             


Ser Ile  Ser Arg Asp Glu Leu  Ile Lys Ala Gly Leu  His Leu Tyr 
    1625                 1630                 1635             


Gly Thr  Asp Gly Asn Asp Glu  Ile Asn Asp His Ala  Asp Trp Asp 
    1640                 1645                 1650             


Ser Ile  Leu Glu Gly Gly Lys  Gly Asn Asp Ile Leu  Arg Gly Ser 
    1655                 1660                 1665             


Tyr Gly  Ala Asp Thr Tyr Ile  Phe Ser Lys Gly His  Gly Gln Asp 
    1670                 1675                 1680             


Val Ile  Tyr Glu Tyr Ser Asp  Ser Ala Asn Ser Lys  Arg Asp Ile 
    1685                 1690                 1695             


Asp Thr  Leu Lys Phe Thr Asp  Val Asn Tyr Ala Glu  Val Lys Phe 
    1700                 1705                 1710             


Arg Arg  Val Gly Asp Asp Leu  Met Leu Phe Gly Tyr  His Asp Thr 
    1715                 1720                 1725             


Asp Ser  Val Thr Val Lys Ser  Phe Tyr Asp His Glu  Tyr Tyr Gln 
    1730                 1735                 1740             


Phe Glu  Lys Leu Glu Phe Ala  Asp Arg Ser Ile Thr  Arg Asp Glu 
    1745                 1750                 1755             


Leu Gly  Lys Gln Gly Met Ala  Leu Phe Gly Thr Asp  Gly Asp Asp 
    1760                 1765                 1770             


Asp Ile  Asn Asp Trp Gly Arg  Asn Ser Val Ile Asp  Ala Gly Ala 
    1775                 1780                 1785             


Gly Asn  Asp Thr Ile Asn Gly  Gly Tyr Gly Asp Asp  Thr Leu Ile 
    1790                 1795                 1800             


Gly Gly  Lys Gly Asn Asp Ile  Leu Lys Gly Ser Tyr  Gly Ala Asp 
    1805                 1810                 1815             


Thr Tyr  Leu Phe Ser Lys Gly  His Gly Gln Asp Val  Ile Tyr Glu 
    1820                 1825                 1830             


Tyr Ser  Asp Ser Ala Ser Ser  Lys Ser Asp Ile Asp  Thr Leu Lys 
    1835                 1840                 1845             


Phe Thr  Asp Ile Gly Leu Ser  Glu Leu Trp Phe Ser  Arg Glu Asn 
    1850                 1855                 1860             


Asn Asp  Leu Ile Ile Lys Ser  Leu Leu Ser Glu Asp  Lys Val Thr 
    1865                 1870                 1875             


Val Gln  Asn Trp Tyr Ser His  Gln Asp His Lys Ile  Glu Asn Ile 
    1880                 1885                 1890             


Arg Leu  Ser Asn Glu Gln Met  Leu Val Ser Thr Gln  Val Glu Lys 
    1895                 1900                 1905             


Met Val  Glu Ser Met Ala Gly  Phe Ala Gln Gln His  Gly Gly Glu 
    1910                 1915                 1920             


Ile Ser  Leu Val Pro Arg Glu  Glu Val Lys Gln Tyr  Ile Asn Ser 
    1925                 1930                 1935             


Leu Thr  Ala Ala Leu 
    1940             


<210>  48
<211>  1086
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APP ApxIV with N-terminal in-frame deletion

<400>  48

Met Thr Lys Leu Thr Met Gln Asp Val Thr Asn Leu Tyr Leu Tyr Lys 
1               5                   10                  15      


Gln Arg Thr Leu Pro Thr Asp Arg Leu Asp Asp Ser Leu Ile Ser Lys 
            20                  25                  30          


Thr Gly Lys Gly Glu Asn Ile Asp Lys Lys Glu Phe Met Ala Gly Pro 
        35                  40                  45              


Gly Arg Phe Val Thr Ala Asp Asn Phe Ser Val Val Lys Asp Phe Phe 
    50                  55                  60                  


Thr Ala Lys Asp Ser Leu Ile Asn Leu Ser Leu Gln Thr Arg Ile Leu 
65                  70                  75                  80  


Ala Asn Leu Lys Pro Gly Lys Tyr Ser Lys Ala Gln Ile Leu Glu Met 
                85                  90                  95      


Leu Gly Tyr Thr Lys Asn Gly Glu Lys Val Asp Gly Met Phe Thr Gly 
            100                 105                 110         


Glu Val Gln Thr Leu Gly Phe Tyr Asp Asp Gly Lys Gly Asp Leu Leu 
        115                 120                 125             


Glu Arg Gln Phe Glu Gly Lys Trp Val Thr Asp Tyr Ser Arg Thr Glu 
    130                 135                 140                 


Ala Leu Phe Asn Ser Thr Phe Lys Gln Ser Pro Glu Asn Ala Leu Tyr 
145                 150                 155                 160 


Asp Leu Ser Glu Tyr Leu Ser Phe Phe Asn Asp Pro Thr Glu Trp Lys 
                165                 170                 175     


Glu Gly Leu Leu Leu Leu Ser Arg Tyr Ile Asp Tyr Ala Lys Ala Gln 
            180                 185                 190         


Gly Phe Tyr Glu Asn Trp Ala Ala Thr Ser Asn Leu Thr Ile Ala Arg 
        195                 200                 205             


Leu Arg Glu Ala Gly Val Ile Phe Ala Glu Ser Thr Asp Leu Lys Gly 
    210                 215                 220                 


Asp Glu Lys Asn Asn Ile Leu Leu Gly Ser Gln Lys Asp Asn Asn Leu 
225                 230                 235                 240 


Ser Gly Ser Ala Gly Asp Asp Leu Leu Ile Gly Gly Glu Gly Asn Asp 
                245                 250                 255     


Thr Leu Lys Gly Ser Tyr Gly Ala Asp Thr Tyr Ile Phe Ser Lys Gly 
            260                 265                 270         


His Gly Gln Asp Ile Val Tyr Glu Asp Thr Asn Asn Asp Asn Arg Ala 
        275                 280                 285             


Arg Asp Ile Asp Thr Leu Lys Phe Thr Asp Val Asn Tyr Ala Glu Val 
    290                 295                 300                 


Lys Phe Arg Arg Val Asp Asn Asp Leu Met Leu Phe Gly Tyr His Asp 
305                 310                 315                 320 


Thr Asp Ser Val Thr Val Lys Ser Phe Tyr Asn His Glu Tyr Tyr Gln 
                325                 330                 335     


Phe Glu Lys Leu Glu Phe Ala Asp Arg Ser Ile Thr Arg Asp Glu Leu 
            340                 345                 350         


Gly Lys Gln Gly Met Ala Leu Phe Gly Thr Asp Gly Asp Asp Asp Ile 
        355                 360                 365             


Asn Asp Trp Gly Arg Asn Ser Val Ile Asp Ala Gly Ala Gly Asn Asp 
    370                 375                 380                 


Thr Ile Asn Gly Ser Tyr Gly Asp Asp Thr Leu Ile Gly Gly Thr Gly 
385                 390                 395                 400 


Asn Asp Ile Leu Lys Gly Ser Tyr Gly Ala Asp Thr Tyr Leu Phe Ser 
                405                 410                 415     


Lys Gly His Gly Gln Asp Val Ile Tyr Glu Tyr Ser Asp Ser Ala Asn 
            420                 425                 430         


Ser Lys Arg Asp Ile Asp Thr Leu Lys Phe Thr Asp Val Asn Tyr Ala 
        435                 440                 445             


Glu Val Lys Phe Arg Arg Val Gly Asp Asp Leu Met Leu Phe Gly Tyr 
    450                 455                 460                 


His Asp Thr Asp Ser Val Thr Val Lys Ser Phe Tyr Asp His Glu Tyr 
465                 470                 475                 480 


Tyr Gln Phe Glu Lys Leu Glu Phe Ala Asp Arg Ser Ile Ser Arg Asp 
                485                 490                 495     


Glu Leu Ile Lys Ala Gly Leu His Leu Tyr Gly Thr Asp Gly Asn Asp 
            500                 505                 510         


Glu Ile Asn Asp His Ala Asp Trp Asp Ser Ile Leu Glu Gly Gly Lys 
        515                 520                 525             


Gly Asn Asp Ile Leu Arg Gly Ser Tyr Gly Ala Asp Thr Tyr Leu Phe 
    530                 535                 540                 


Ser Lys Gly His Gly Gln Asp Val Ile Tyr Glu Tyr Ser Asp Ser Ala 
545                 550                 555                 560 


Asn Ser Lys Ser Asp Ile Asp Thr Leu Lys Phe Thr Asp Ile Asn Tyr 
                565                 570                 575     


Ala Glu Val Lys Phe Arg Arg Val Gly Asp Asp Leu Met Leu Phe Gly 
            580                 585                 590         


Tyr His Asp Thr Asp Ser Val Thr Val Lys Ser Phe Tyr Asn His Glu 
        595                 600                 605             


Tyr Tyr Gln Phe Glu Lys Leu Glu Phe Ala Asp Arg Ser Ile Thr Arg 
    610                 615                 620                 


Asp Glu Leu Gly Lys Gln Gly Met Ala Leu Phe Gly Thr Asp Gly Asp 
625                 630                 635                 640 


Asp Asp Ile Asn Asp Trp Gly Arg Asn Ser Val Ile Asp Ala Gly Ala 
                645                 650                 655     


Gly Asn Asp Thr Ile Asn Gly Ser Tyr Gly Asp Asp Thr Leu Ile Gly 
            660                 665                 670         


Gly Thr Gly Asn Asp Ile Leu Lys Gly Ser Tyr Gly Ala Asp Thr Tyr 
        675                 680                 685             


Leu Phe Ser Lys Gly His Gly Gln Asp Val Ile Tyr Glu Tyr Ser Asp 
    690                 695                 700                 


Ser Ala Asn Ser Lys Arg Asp Ile Asp Thr Leu Lys Phe Thr Asp Val 
705                 710                 715                 720 


Asn Tyr Ala Glu Val Lys Phe Arg Arg Val Gly Asp Asp Leu Met Leu 
                725                 730                 735     


Phe Gly Tyr His Asp Thr Asp Ser Val Thr Val Lys Ser Phe Tyr Ser 
            740                 745                 750         


His Glu Tyr Tyr Gln Phe Glu Lys Leu Glu Phe Ala Asp Arg Ser Ile 
        755                 760                 765             


Ser Arg Asp Glu Leu Ile Lys Ala Gly Leu His Leu Tyr Gly Thr Asp 
    770                 775                 780                 


Gly Asn Asp Glu Ile Asn Asp His Ala Asp Trp Asp Ser Ile Leu Glu 
785                 790                 795                 800 


Gly Gly Lys Gly Asn Asp Ile Leu Arg Gly Ser Tyr Gly Ala Asp Thr 
                805                 810                 815     


Tyr Ile Phe Ser Lys Gly His Gly Gln Asp Val Ile Tyr Glu Tyr Ser 
            820                 825                 830         


Asp Ser Ala Asn Ser Lys Arg Asp Ile Asp Thr Leu Lys Phe Thr Asp 
        835                 840                 845             


Val Asn Tyr Ala Glu Val Lys Phe Arg Arg Val Gly Asp Asp Leu Met 
    850                 855                 860                 


Leu Phe Gly Tyr His Asp Thr Asp Ser Val Thr Val Lys Ser Phe Tyr 
865                 870                 875                 880 


Asp His Glu Tyr Tyr Gln Phe Glu Lys Leu Glu Phe Ala Asp Arg Ser 
                885                 890                 895     


Ile Thr Arg Asp Glu Leu Gly Lys Gln Gly Met Ala Leu Phe Gly Thr 
            900                 905                 910         


Asp Gly Asp Asp Asp Ile Asn Asp Trp Gly Arg Asn Ser Val Ile Asp 
        915                 920                 925             


Ala Gly Ala Gly Asn Asp Thr Ile Asn Gly Gly Tyr Gly Asp Asp Thr 
    930                 935                 940                 


Leu Ile Gly Gly Lys Gly Asn Asp Ile Leu Lys Gly Ser Tyr Gly Ala 
945                 950                 955                 960 


Asp Thr Tyr Leu Phe Ser Lys Gly His Gly Gln Asp Val Ile Tyr Glu 
                965                 970                 975     


Tyr Ser Asp Ser Ala Ser Ser Lys Ser Asp Ile Asp Thr Leu Lys Phe 
            980                 985                 990         


Thr Asp Ile Gly Leu Ser Glu Leu  Trp Phe Ser Arg Glu  Asn Asn Asp 
        995                 1000                 1005             


Leu Ile  Ile Lys Ser Leu Leu  Ser Glu Asp Lys Val  Thr Val Gln 
    1010                 1015                 1020             


Asn Trp  Tyr Ser His Gln Asp  His Lys Ile Glu Asn  Ile Arg Leu 
    1025                 1030                 1035             


Ser Asn  Glu Gln Met Leu Val  Ser Thr Gln Val Glu  Lys Met Val 
    1040                 1045                 1050             


Glu Ser  Met Ala Gly Phe Ala  Gln Gln His Gly Gly  Glu Ile Ser 
    1055                 1060                 1065             


Leu Val  Pro Arg Glu Glu Val  Lys Gln Tyr Ile Asn  Ser Leu Thr 
    1070                 1075                 1080             


Ala Ala  Leu 
    1085     


