                         SEQUENCE LISTING

<110>  CycleZyme AB
 
<120>  Thermostable engineered IsPETases

<130>  P5750PC00

<150>  EP 20193259.7
<151>  2020-08-28

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  Xaa = Hydrogen bond donating amino acid, such as Arg, Lys, or His

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  Xaa = Negatively charged amino acid, such as Asp or Glu

<400>  1

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Xaa Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Xaa Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  2
<211>  262
<212>  PRT
<213>  Ideonella sakaiensis

<400>  2

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Ser Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  3
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Arg

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Asp

<400>  3

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Arg Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  4
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Lys

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Asp

<400>  4

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Lys Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  5
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = His

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Asp

<400>  5

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro His Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  6
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Leu

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Asp

<400>  6

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Leu Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  7
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Arg

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Glu

<400>  7

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Arg Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Glu Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  8
<211>  261
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Lys

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Glu

<400>  8

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Lys Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Glu Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys 
            260     


<210>  9
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = His

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Glu

<400>  9

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro His Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Glu Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  10
<211>  262
<212>  PRT
<213>  Artifical sequence


<220>
<221>  MISC_FEATURE
<222>  (93)..(93)
<223>  X1 = Leu

<220>
<221>  MISC_FEATURE
<222>  (158)..(158)
<223>  X2 = Glu

<400>  10

Met Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu 
1               5                   10                  15      


Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro 
            20                  25                  30          


Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly 
        35                  40                  45              


Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser 
    50                  55                  60                  


Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val 
65                  70                  75                  80  


Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro Leu Ser Arg Ser 
                85                  90                  95      


Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr 
            100                 105                 110         


Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val 
        115                 120                 125             


Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn 
    130                 135                 140                 


Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro Trp Glu Ser Ser 
145                 150                 155                 160 


Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu 
                165                 170                 175     


Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp 
            180                 185                 190         


Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser 
        195                 200                 205             


His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys 
    210                 215                 220                 


Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr 
225                 230                 235                 240 


Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe 
                245                 250                 255     


Arg Thr Ala Asn Cys Ser 
            260         


<210>  11
<211>  426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant


<220>
<221>  MISC_FEATURE
<222>  (257)..(257)
<223>  S to H

<400>  11

Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp 
1               5                   10                  15      


Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp 
            20                  25                  30          


Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp 
        35                  40                  45              


Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn 
    50                  55                  60                  


Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu 
65                  70                  75                  80  


Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser 
                85                  90                  95      


Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly 
            100                 105                 110         


Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro 
        115                 120                 125             


Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln 
    130                 135                 140                 


His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 
145                 150                 155                 160 


Ala Ile Ser Asp Pro Asn Pro Tyr Ala Arg Gly Pro Asn Pro Thr Ala 
                165                 170                 175     


Ala Ser Leu Glu Ala Ser Ala Gly Pro Phe Thr Val Arg Ser Phe Thr 
            180                 185                 190         


Val Ser Arg Pro Ser Gly Tyr Gly Ala Gly Thr Val Tyr Tyr Pro Thr 
        195                 200                 205             


Asn Ala Gly Gly Thr Val Gly Ala Ile Ala Ile Val Pro Gly Tyr Thr 
    210                 215                 220                 


Ala Arg Gln Ser Ser Ile Lys Trp Trp Gly Pro Arg Leu Ala Ser His 
225                 230                 235                 240 


Gly Phe Val Val Ile Thr Ile Asp Thr Asn Ser Thr Leu Asp Gln Pro 
                245                 250                 255     


His Ser Arg Ser Ser Gln Gln Met Ala Ala Leu Arg Gln Val Ala Ser 
            260                 265                 270         


Leu Asn Gly Thr Ser Ser Ser Pro Ile Tyr Gly Lys Val Asp Thr Ala 
        275                 280                 285             


Arg Met Gly Val Met Gly Trp Ser Met Gly Gly Gly Gly Ser Leu Ile 
    290                 295                 300                 


Ser Ala Ala Asn Asn Pro Ser Leu Lys Ala Ala Ala Pro Gln Ala Pro 
305                 310                 315                 320 


Trp Asp Ser Ser Thr Asn Phe Ser Ser Val Thr Val Pro Thr Leu Ile 
                325                 330                 335     


Phe Ala Cys Glu Asn Asp Ser Ile Ala Pro Val Asn Ser Ser Ala Leu 
            340                 345                 350         


Pro Ile Tyr Asp Ser Met Ser Arg Asn Ala Lys Gln Phe Leu Glu Ile 
        355                 360                 365             


Asn Gly Gly Ser His Ser Cys Ala Asn Ser Gly Asn Ser Asn Gln Ala 
    370                 375                 380                 


Leu Ile Gly Lys Lys Gly Val Ala Trp Met Lys Arg Phe Met Asp Asn 
385                 390                 395                 400 


Asp Thr Arg Tyr Ser Thr Phe Ala Cys Glu Asn Pro Asn Ser Thr Arg 
                405                 410                 415     


Val Ser Asp Phe Arg Thr Ala Asn Cys Ser 
            420                 425     


