                         SEQUENCE LISTING

<110>  NGM BIOPHARMACEUTICALS, INC.
 
<120>  BAMA-BINDING AGENTS AND METHODS OF USE THEREOF

<130>  13370-115-228

<140>  TBA
<141>  On even date herewith

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  841
<212>  PRT
<213>  Acinetobacter baumannii

<220>
<223>  Acinetobacter baumannii BamA(ATCC 19606)

<400>  1

Met Arg His Thr His Phe Leu Met Pro Leu Ala Leu Val Ser Ala Met 
1               5                   10                  15      


Ala Ala Val Gln Gln Ala Tyr Ala Ala Asp Asp Phe Val Val Arg Asp 
            20                  25                  30          


Ile Arg Val Asn Gly Leu Val Arg Leu Thr Pro Ala Asn Val Tyr Thr 
        35                  40                  45              


Met Leu Pro Ile Asn Ser Gly Asp Arg Val Asn Glu Pro Met Ile Ala 
    50                  55                  60                  


Glu Ala Ile Arg Thr Leu Tyr Ala Thr Gly Leu Phe Asp Asp Ile Lys 
65                  70                  75                  80  


Ala Ser Lys Glu Asn Asp Thr Leu Val Phe Asn Val Ile Glu Arg Pro 
                85                  90                  95      


Ile Ile Ser Lys Leu Glu Phe Lys Gly Asn Lys Leu Ile Pro Lys Glu 
            100                 105                 110         


Ala Leu Glu Gln Gly Leu Lys Lys Met Gly Ile Ala Glu Gly Glu Val 
        115                 120                 125             


Phe Lys Lys Ser Ala Leu Gln Thr Ile Glu Thr Glu Leu Glu Gln Gln 
    130                 135                 140                 


Tyr Thr Gln Gln Gly Arg Tyr Asp Ala Asp Val Thr Val Asp Thr Val 
145                 150                 155                 160 


Ala Arg Pro Asn Asn Arg Val Glu Leu Lys Ile Asn Phe Asn Glu Gly 
                165                 170                 175     


Thr Pro Ala Lys Val Phe Asp Ile Asn Val Ile Gly Asn Thr Val Phe 
            180                 185                 190         


Lys Asp Ser Glu Ile Lys Gln Ala Phe Ala Val Lys Glu Ser Gly Trp 
        195                 200                 205             


Ala Ser Val Val Thr Arg Asn Asp Arg Tyr Ala Arg Glu Lys Met Ala 
    210                 215                 220                 


Ala Ser Leu Glu Ala Leu Arg Ala Met Tyr Leu Asn Lys Gly Tyr Ile 
225                 230                 235                 240 


Asn Phe Asn Ile Asn Asn Ser Gln Leu Asn Ile Ser Glu Asp Lys Lys 
                245                 250                 255     


His Ile Phe Ile Glu Val Ala Val Asp Glu Gly Ser Gln Phe Lys Phe 
            260                 265                 270         


Gly Gln Thr Lys Phe Leu Gly Asp Ala Leu Tyr Lys Pro Glu Glu Leu 
        275                 280                 285             


Gln Ala Leu Lys Ile Tyr Lys Asp Gly Asp Thr Tyr Ser Gln Glu Lys 
    290                 295                 300                 


Val Asn Ala Val Lys Gln Leu Leu Leu Arg Lys Tyr Gly Asn Ala Gly 
305                 310                 315                 320 


Tyr Tyr Phe Ala Asp Val Asn Ile Val Pro Gln Ile Asn Asn Glu Thr 
                325                 330                 335     


Gly Val Val Asp Leu Asn Tyr Tyr Val Asn Pro Gly Gln Gln Val Thr 
            340                 345                 350         


Val Arg Arg Ile Asn Phe Thr Gly Asn Ser Lys Thr Ser Asp Glu Val 
        355                 360                 365             


Leu Arg Arg Glu Met Arg Gln Met Glu Gly Ala Leu Ala Ser Asn Glu 
    370                 375                 380                 


Lys Ile Asp Leu Ser Lys Val Arg Leu Glu Arg Thr Gly Phe Phe Lys 
385                 390                 395                 400 


Thr Val Asp Ile Lys Pro Ala Arg Ile Pro Asn Ser Pro Asp Gln Val 
                405                 410                 415     


Asp Leu Asn Val Asn Val Glu Glu Gln His Ser Gly Thr Thr Thr Leu 
            420                 425                 430         


Ala Val Gly Tyr Ser Gln Ser Gly Gly Ile Thr Phe Gln Ala Gly Leu 
        435                 440                 445             


Ser Gln Thr Asn Phe Met Gly Thr Gly Asn Arg Val Ala Ile Asp Leu 
    450                 455                 460                 


Ser Arg Ser Glu Thr Gln Asp Tyr Tyr Asn Leu Ser Val Thr Asp Pro 
465                 470                 475                 480 


Tyr Phe Thr Ile Asp Gly Val Ser Arg Gly Tyr Asn Val Tyr Tyr Arg 
                485                 490                 495     


Lys Thr Lys Leu Asn Asp Asp Tyr Asn Val Asn Asn Tyr Val Thr Asp 
            500                 505                 510         


Ser Phe Gly Gly Ser Leu Ser Phe Gly Tyr Pro Ile Asp Glu Asn Gln 
        515                 520                 525             


Ser Leu Ser Ala Ser Val Gly Val Asp Asn Thr Lys Val Thr Thr Gly 
    530                 535                 540                 


Pro Tyr Val Ser Thr Tyr Val Arg Asp Tyr Leu Leu Ala Asn Gly Gly 
545                 550                 555                 560 


Lys Ala Thr Ser Lys Gly Thr Tyr Cys Pro Thr Asp Ala Asn Gly Asp 
                565                 570                 575     


Ser Gln Tyr Asp Thr Glu Lys Gly Glu Cys Lys Val Pro Glu Glu Thr 
            580                 585                 590         


Tyr Asp Asn Ala Phe Glu Gly Glu Phe Phe Thr Tyr Asn Leu Asn Leu 
        595                 600                 605             


Gly Trp Ser Tyr Asn Thr Leu Asn Arg Pro Ile Phe Pro Thr Ser Gly 
    610                 615                 620                 


Met Ser His Arg Val Gly Leu Glu Ile Gly Leu Pro Gly Ser Asp Val 
625                 630                 635                 640 


Asp Tyr Gln Lys Val Thr Tyr Asp Thr Gln Ala Phe Phe Pro Ile Gly 
                645                 650                 655     


Ser Thr Gly Phe Val Leu Arg Gly Tyr Gly Lys Leu Gly Tyr Gly Asn 
            660                 665                 670         


Asp Leu Pro Phe Tyr Lys Asn Phe Tyr Ala Gly Gly Tyr Gly Ser Val 
        675                 680                 685             


Arg Gly Tyr Asp Asn Ser Thr Leu Gly Pro Lys Tyr Pro Ser Val Asn 
    690                 695                 700                 


Leu Gln Glu Thr Lys Gln Asn Asp Ser Ser Pro Glu Glu Val Gly Gly 
705                 710                 715                 720 


Asn Ala Leu Val Gln Phe Gly Thr Glu Leu Val Leu Pro Met Pro Phe 
                725                 730                 735     


Lys Gly Asp Trp Thr Arg Gln Val Arg Pro Val Leu Phe Ala Glu Gly 
            740                 745                 750         


Gly Gln Val Phe Asp Thr Lys Cys Asn Ile Asp Asn Ser Val Tyr Gly 
        755                 760                 765             


Asn Lys Gly Met Lys Ile Asn Gly Gln Thr Ile Thr Asp Val Arg Lys 
    770                 775                 780                 


Tyr Cys Glu Asp Asn Tyr Gly Phe Asp Leu Gly Asn Leu Arg Tyr Ser 
785                 790                 795                 800 


Val Gly Val Gly Val Thr Trp Ile Thr Met Ile Gly Pro Leu Ser Leu 
                805                 810                 815     


Ser Tyr Ala Phe Pro Leu Asn Asp Lys Pro Gly Asp Glu Thr Lys Glu 
            820                 825                 830         


Ile Gln Phe Glu Ile Gly Arg Thr Phe 
        835                 840     


<210>  2
<211>  415
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain (aa 427-841)  

<400>  2

Ser Gly Thr Thr Thr Leu Ala Val Gly Tyr Ser Gln Ser Gly Gly Ile 
1               5                   10                  15      


Thr Phe Gln Ala Gly Leu Ser Gln Thr Asn Phe Met Gly Thr Gly Asn 
            20                  25                  30          


Arg Val Ala Ile Asp Leu Ser Arg Ser Glu Thr Gln Asp Tyr Tyr Asn 
        35                  40                  45              


Leu Ser Val Thr Asp Pro Tyr Phe Thr Ile Asp Gly Val Ser Arg Gly 
    50                  55                  60                  


Tyr Asn Val Tyr Tyr Arg Lys Thr Lys Leu Asn Asp Asp Tyr Asn Val 
65                  70                  75                  80  


Asn Asn Tyr Val Thr Asp Ser Phe Gly Gly Ser Leu Ser Phe Gly Tyr 
                85                  90                  95      


Pro Ile Asp Glu Asn Gln Ser Leu Ser Ala Ser Val Gly Val Asp Asn 
            100                 105                 110         


Thr Lys Val Thr Thr Gly Pro Tyr Val Ser Thr Tyr Val Arg Asp Tyr 
        115                 120                 125             


Leu Leu Ala Asn Gly Gly Lys Ala Thr Ser Lys Gly Thr Tyr Cys Pro 
    130                 135                 140                 


Thr Asp Ala Asn Gly Asp Ser Gln Tyr Asp Thr Glu Lys Gly Glu Cys 
145                 150                 155                 160 


Lys Val Pro Glu Glu Thr Tyr Asp Asn Ala Phe Glu Gly Glu Phe Phe 
                165                 170                 175     


Thr Tyr Asn Leu Asn Leu Gly Trp Ser Tyr Asn Thr Leu Asn Arg Pro 
            180                 185                 190         


Ile Phe Pro Thr Ser Gly Met Ser His Arg Val Gly Leu Glu Ile Gly 
        195                 200                 205             


Leu Pro Gly Ser Asp Val Asp Tyr Gln Lys Val Thr Tyr Asp Thr Gln 
    210                 215                 220                 


Ala Phe Phe Pro Ile Gly Ser Thr Gly Phe Val Leu Arg Gly Tyr Gly 
225                 230                 235                 240 


Lys Leu Gly Tyr Gly Asn Asp Leu Pro Phe Tyr Lys Asn Phe Tyr Ala 
                245                 250                 255     


Gly Gly Tyr Gly Ser Val Arg Gly Tyr Asp Asn Ser Thr Leu Gly Pro 
            260                 265                 270         


Lys Tyr Pro Ser Val Asn Leu Gln Glu Thr Lys Gln Asn Asp Ser Ser 
        275                 280                 285             


Pro Glu Glu Val Gly Gly Asn Ala Leu Val Gln Phe Gly Thr Glu Leu 
    290                 295                 300                 


Val Leu Pro Met Pro Phe Lys Gly Asp Trp Thr Arg Gln Val Arg Pro 
305                 310                 315                 320 


Val Leu Phe Ala Glu Gly Gly Gln Val Phe Asp Thr Lys Cys Asn Ile 
                325                 330                 335     


Asp Asn Ser Val Tyr Gly Asn Lys Gly Met Lys Ile Asn Gly Gln Thr 
            340                 345                 350         


Ile Thr Asp Val Arg Lys Tyr Cys Glu Asp Asn Tyr Gly Phe Asp Leu 
        355                 360                 365             


Gly Asn Leu Arg Tyr Ser Val Gly Val Gly Val Thr Trp Ile Thr Met 
    370                 375                 380                 


Ile Gly Pro Leu Ser Leu Ser Tyr Ala Phe Pro Leu Asn Asp Lys Pro 
385                 390                 395                 400 


Gly Asp Glu Thr Lys Glu Ile Gln Phe Glu Ile Gly Arg Thr Phe 
                405                 410                 415 


<210>  3
<211>  5
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain loop 1 (aa 438-442)  

<400>  3

Gln Ser Gly Gly Ile 
1               5   


<210>  4
<211>  6
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain loop 2 (aa 467-472)  

<400>  4

Ser Glu Thr Gln Asp Tyr 
1               5       


<210>  5
<211>  19
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain loop 3 (aa 496-514)  

<400>  5

Arg Lys Thr Lys Leu Asn Asp Asp Tyr Asn Val Asn Asn Tyr Val Thr 
1               5                   10                  15      


Asp Ser Phe 
            


<210>  6
<211>  66
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain loop 4 (aa 538-603)  

<400>  6

Asn Thr Lys Val Thr Thr Gly Pro Tyr Val Ser Thr Tyr Val Arg Asp 
1               5                   10                  15      


Tyr Leu Leu Ala Asn Gly Gly Lys Ala Thr Ser Lys Gly Thr Tyr Cys 
            20                  25                  30          


Pro Thr Asp Ala Asn Gly Asp Ser Gln Tyr Asp Thr Glu Lys Gly Glu 
        35                  40                  45              


Cys Lys Val Pro Glu Glu Thr Tyr Asp Asn Ala Phe Glu Gly Glu Phe 
    50                  55                  60                  


Phe Thr 
65      


<210>  7
<211>  9
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel domain 
loop 5 (aa 635-643)  

<400>  7

Leu Pro Gly Ser Asp Val Asp Tyr Gln 
1               5                   


<210>  8
<211>  52
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel domain 
loop 6 (aa 672-723)  

<400>  8

Asn Asp Leu Pro Phe Tyr Lys Asn Phe Tyr Ala Gly Gly Tyr Gly Ser 
1               5                   10                  15      


Val Arg Gly Tyr Asp Asn Ser Thr Leu Gly Pro Lys Tyr Pro Ser Val 
            20                  25                  30          


Asn Leu Gln Glu Thr Lys Gln Asn Asp Ser Ser Pro Glu Glu Val Gly 
        35                  40                  45              


Gly Asn Ala Leu 
    50          


<210>  9
<211>  46
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel 
domain loop 7 (aa 754-799)  

<400>  9

Gln Val Phe Asp Thr Lys Cys Asn Ile Asp Asn Ser Val Tyr Gly Asn 
1               5                   10                  15      


Lys Gly Met Lys Ile Asn Gly Gln Thr Ile Thr Asp Val Arg Lys Tyr 
            20                  25                  30          


Cys Glu Asp Asn Tyr Gly Phe Asp Leu Gly Asn Leu Arg Tyr 
        35                  40                  45      


<210>  10
<211>  12
<212>  PRT
<213>  Acinetobacter baumannii 

<220>
<223>  Acinetobacter baumannii (ATCC 19606) Predicted barrel domain 
loop 8 (aa 821-832)  

<400>  10

Pro Leu Asn Asp Lys Pro Gly Asp Glu Thr Lys Glu 
1               5                   10          


<210>  11
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR1-Exemplary and AbM

<400>  11

Gly Leu Ser Phe Gly Leu Asp Ala Tyr Ala Val Ala 
1               5                   10          


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR2-Exemplary and Kabat

<400>  12

Cys Ile Ser Pro Thr Gly Ser Arg Val Ala Tyr Ala Asp Ser Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR3-Exemplary, Chothia, 
AbM and Kabat

<400>  13

Ser Asn Asp Lys Arg Cys Ser Asp Phe Gly Val Asp Arg Val Gly Tyr 
1               5                   10                  15      


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR1 -Chothia

<400>  14

Gly Leu Ser Phe Gly Leu Asp Ala Tyr 
1               5                   


<210>  15
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR2 - Chothia

<400>  15

Ser Pro Thr Gly Ser Arg 
1               5       


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR2 -AbM

<400>  16

Cys Ile Ser Pro Thr Gly Ser Arg Val Ala 
1               5                   10  


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR1 -Kabat

<400>  17

Leu Asp Ala Tyr Ala Val Ala 
1               5           


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR1 -Contact

<400>  18

Gly Leu Asp Ala Tyr Ala Val Ala 
1               5               


<210>  19
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR2 -Contact

<400>  19

Gly Val Ser Cys Ile Ser Pro Thr Gly Ser Arg Val Ala 
1               5                   10              


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29 Heavy Chain variable region CDR3 -Contact

<400>  20

Ala Thr Ser Asn Asp Lys Arg Cys Ser Asp Phe Gly Val Asp Arg Val 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR1-Exemplary and AbM

<400>  21

Gly Ser Thr Ala Thr Arg Asp Thr Phe Ser Ser His Arg Met Thr 
1               5                   10                  15  


<210>  22
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR2 -Exemplary and Kabat

<400>  22

Thr Ile Thr Gly Asp Asp Ile Thr Asn Tyr Thr Gly Ser Val Lys Gly 
1               5                   10                  15      


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR3 -Exemplary, 
Chothia, AbM and Kabat

<400>  23

Leu Glu Arg Gly Ile Trp Ala Tyr 
1               5               


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR1 -Chothia

<400>  24

Gly Ser Thr Ala Thr Arg Asp Thr Phe Ser Ser His 
1               5                   10          


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR2 -Chothia

<400>  25

Thr Gly Asp Asp Ile 
1               5   


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR2 -AbM

<400>  26

Thr Ile Thr Gly Asp Asp Ile Thr Asn 
1               5                   


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR1 -Kabat

<400>  27

Arg Asp Thr Phe Ser Ser His Arg Met Thr 
1               5                   10  


<210>  28
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR1 -Contact

<400>  28

Thr Arg Asp Thr Phe Ser Ser His Arg Met Thr 
1               5                   10      


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR2 -Contact

<400>  29

Met Val Ala Thr Ile Thr Gly Asp Asp Ile Thr Asn 
1               5                   10          


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47 Heavy Chain variable region CDR3 -Contact

<400>  30

His Leu Leu Glu Arg Gly Ile Trp Ala 
1               5                   


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR1 -
Exemplary and AbM

<400>  31

Gly Phe Ser Phe Arg Asp Tyr Ser Met Ser 
1               5                   10  


<210>  32
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR2-Exemplary and Kabat

<400>  32

Gly Ile Arg Ser Leu Gly Thr Thr Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  33
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR3 -Exemplary, 
Chothia, AbM and Kabat

<400>  33

Cys Leu Gly Lys Ile Cys Asp Arg Phe Gly Ile Val Asp Ile Tyr Ser 
1               5                   10                  15      


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR1 -Chothia 

<400>  34

Gly Phe Ser Phe Arg Asp Tyr 
1               5           


<210>  35
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR2 -Chothia

<400>  35

Arg Ser Leu Gly Thr Thr 
1               5       


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR2 -AbM 

<400>  36

Gly Ile Arg Ser Leu Gly Thr Thr Thr Tyr 
1               5                   10  


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR1 -Kabat

<400>  37

Asp Tyr Ser Met Ser 
1               5   


<210>  38
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR1 -Contact

<400>  38

Arg Asp Tyr Ser Met Ser 
1               5       


<210>  39
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR2 -Contact

<400>  39

Trp Val Ser Gly Ile Arg Ser Leu Gly Thr Thr Thr Tyr 
1               5                   10              


<210>  40
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82 Heavy Chain variable region CDR3 -Contact

<400>  40

Ala Lys Cys Leu Gly Lys Ile Cys Asp Arg Phe Gly Ile Val Asp Ile 
1               5                   10                  15      


Tyr 
    


<210>  41
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-29

<400>  41

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Leu Ser Phe Gly Leu Asp 
            20                  25                  30          


Ala Tyr Ala Val Ala Trp Phe Arg Gln Val Pro Gly Lys Gly Arg Glu 
        35                  40                  45              


Gly Val Ser Cys Ile Ser Pro Thr Gly Ser Arg Val Ala Tyr Ala Asp 
    50                  55                  60                  


Ser Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Val Gln Lys Thr 
65                  70                  75                  80  


Val Ala Leu Gln Ile His Thr Leu Lys Pro Glu Asp Thr Ala Lys Tyr 
                85                  90                  95      


Phe Cys Ala Thr Ser Asn Asp Lys Arg Cys Ser Asp Phe Gly Val Asp 
            100                 105                 110         


Arg Val Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  42
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-47

<400>  42

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Ser Thr Ala Thr Arg Asp 
            20                  25                  30          


Thr Phe Ser Ser His Arg Met Thr Trp Tyr Arg Gln Ala Pro Gly Lys 
        35                  40                  45              


Gln Arg Glu Met Val Ala Thr Ile Thr Gly Asp Asp Ile Thr Asn Tyr 
    50                  55                  60                  


Thr Gly Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ser Pro Lys 
65                  70                  75                  80  


Lys Thr Met Tyr Leu Gln Met Asn Asn Leu Arg Pro Glu Asp Thr Ala 
                85                  90                  95      


Val Tyr Tyr Cys His Leu Leu Glu Arg Gly Ile Trp Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  43
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH-82

<400>  43

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Phe Arg Asp Tyr 
            20                  25                  30          


Ser Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Arg Ser Leu Gly Thr Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Lys Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Asp Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Cys Leu Gly Lys Ile Cys Asp Arg Phe Gly Ile Val Asp Ile 
            100                 105                 110         


Tyr Ser Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  44
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region - CH1, hinge, CH2, and CH3  

<400>  44

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  45
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region E233A/L235A

<400>  45

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Ala Leu Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  46
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region L234A/L235A

<400>  46

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  47
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region L234A/L235A/P329G

<400>  47

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  48
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region N297G

<400>  48

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Gly Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  49
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region N297G/H310A

<400>  49

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Gly Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


Ala Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  50
<211>  330
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IgG1 constant region L234F/L235E/P331G

<400>  50

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Gly Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  51
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 constant region L234A/L235A/P331G

<400>  51

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Gly Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hexahistidine peptide tag

<400>  52

His His His His His His 
1               5       


