
                                SEQUENCE LISTING

<110> Zhang, Qihong
      Searby, Charles C.
      Sheffield, Val C.
      Carter, Calvin S. PhD
      Huang, Sunny C.
      University of Iowa Research Foundation
      

<120> GENE KNOCK-OUT FOR TREATMENT OF GLAUCOMA
  
  

<130> 875.214WO1

<140> PCT/US2021/048018   
<141> 2021-08-27  

<150> US 63/071,161       
<151> 2020-08-27  

<160> 15

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequecne

<400> 1
gtcagtcatc cataacttac a                                                21

<210> 2
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 2
gaccagctgg aaacccaaac ca                                               22

<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 3
ggctccagag aagtttctac g                                                21

<210> 4

<220> 
<223> A synthetic sequence

<400> 4
 000

<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 5
ggcctgcctg gtgtgggatg                                                  20

<210> 6
<211> 1934
<212> DNA
<213> Homo sapiens

<400> 6
cgggccccgg acacccgctc tgcacagcag agctttccag aggaagcctc accaagcctc      60
tgcaatgagg ttcttctgtg cacgttgctg cagctttggg cctgagatgc cagctgtcca     120
gctgctgctt ctggcctgcc tggtgtggga tgtgggggcc aggacagctc agctcaggaa     180
ggccaatgac cagagtggcc gatgccagta taccttcagt gtggccagtc ccaatgaatc     240
cagctgccca gagcagagcc aggccatgtc agtcatccat aacttacaga gagacagcag     300
cacccaacgc ttagacctgg aggccaccaa agctcgactc agctccctgg agagcctcct     360
ccaccaattg accttggacc aggctgccag gccccaggag acccaggagg ggctgcagag     420
ggagctgggc accctgaggc gggagcggga ccagctggaa acccaaacca gagagttgga     480
gactgcctac agcaacctcc tccgagacaa gtcagttctg gaggaagaga agaagcgact     540
aaggcaagaa aatgagaatc tggccaggag gttggaaagc agcagccagg aggtagcaag     600
gctgagaagg ggccagtgtc cccagacccg agacactgct cgggctgtgc caccaggctc     660
cagagaagtt tctacgtgga atttggacac tttggccttc caggaactga agtccgagct     720
aactgaagtt cctgcttccc gaattttgaa ggagagccca tctggctatc tcaggagtgg     780
agagggagac accggatgtg gagaactagt ttgggtagga gagcctctca cgctgagaac     840
agcagaaaca attactggca agtatggtgt gtggatgcga gaccccaagc ccacctaccc     900
ctacacccag gagaccacgt ggagaatcga cacagttggc acggatgtcc gccaggtttt     960
tgagtatgac ctcatcagcc agtttatgca gggctaccct tctaaggttc acatactgcc    1020
taggccactg gaaagcacgg gtgctgtggt gtactcgggg agcctctatt tccagggcgc    1080
tgagtccaga actgtcataa gatatgagct gaataccgag acagtgaagg ctgagaagga    1140
aatccctgga gctggctacc acggacagtt cccgtattct tggggtggct acacggacat    1200
tgacttggct gtggatgaag caggcctctg ggtcatttac agcaccgatg aggccaaagg    1260
tgccattgtc ctctccaaac tgaacccaga gaatctggaa ctcgaacaaa cctgggagac    1320
aaacatccgt aagcagtcag tcgccaatgc cttcatcatc tgtggcacct tgtacaccgt    1380
cagcagctac acctcagcag atgctaccgt caactttgct tatgacacag gcacaggtat    1440
cagcaagacc ctgaccatcc cattcaagaa ccgctataag tacagcagca tgattgacta    1500
caaccccctg gagaagaagc tctttgcctg ggacaacttg aacatggtca cttatgacat    1560
caagctctcc aagatgtgaa aagcctccaa gctgtacagg caatggcaga aggagatgct    1620
cagggctcct ggggggagca ggctgaaggg agagccagcc agccagggcc caggcagctt    1680
tgactgcttt ccaagttttc attaatccag aaggatgaac atggtcacca tctaactatt    1740
caggaattgt agtctgaggg cgtagacaat ttcatataat aaatatcctt tatcttctgt    1800
cagcatttat gggatgttta atgacatagt tcaagttttc ttgtgatttg gggcaaaagc    1860
tgtaaggcat aatagtttct tcctgaaaac cattgctctt gcatgttaca tgggtaccac    1920
aagccacaat aaaa                                                      1934

<210> 7
<211> 504
<212> PRT
<213> Homo sapiens

<400> 7
Met Arg Phe Phe Cys Ala Arg Cys Cys Ser Phe Gly Pro Glu Met Pro
 1               5                  10                  15
Ala Val Gln Leu Leu Leu Leu Ala Cys Leu Val Trp Asp Val Gly Ala
            20                  25                  30
Arg Thr Ala Gln Leu Arg Lys Ala Asn Asp Gln Ser Gly Arg Cys Gln
        35                  40                  45
Tyr Thr Phe Ser Val Ala Ser Pro Asn Glu Ser Ser Cys Pro Glu Gln
    50                  55                  60
Ser Gln Ala Met Ser Val Ile His Asn Leu Gln Arg Asp Ser Ser Thr
65                  70                  75                  80
Gln Arg Leu Asp Leu Glu Ala Thr Lys Ala Arg Leu Ser Ser Leu Glu
                85                  90                  95
Ser Leu Leu His Gln Leu Thr Leu Asp Gln Ala Ala Arg Pro Gln Glu
            100                 105                 110
Thr Gln Glu Gly Leu Gln Arg Glu Leu Gly Thr Leu Arg Arg Glu Arg
        115                 120                 125
Asp Gln Leu Glu Thr Gln Thr Arg Glu Leu Glu Thr Ala Tyr Ser Asn
    130                 135                 140
Leu Leu Arg Asp Lys Ser Val Leu Glu Glu Glu Lys Lys Arg Leu Arg
145                 150                 155                 160
Gln Glu Asn Glu Asn Leu Ala Arg Arg Leu Glu Ser Ser Ser Gln Glu
                165                 170                 175
Val Ala Arg Leu Arg Arg Gly Gln Cys Pro Gln Thr Arg Asp Thr Ala
            180                 185                 190
Arg Ala Val Pro Pro Gly Ser Arg Glu Val Ser Thr Trp Asn Leu Asp
        195                 200                 205
Thr Leu Ala Phe Gln Glu Leu Lys Ser Glu Leu Thr Glu Val Pro Ala
    210                 215                 220
Ser Arg Ile Leu Lys Glu Ser Pro Ser Gly Tyr Leu Arg Ser Gly Glu
225                 230                 235                 240
Gly Asp Thr Gly Cys Gly Glu Leu Val Trp Val Gly Glu Pro Leu Thr
                245                 250                 255
Leu Arg Thr Ala Glu Thr Ile Thr Gly Lys Tyr Gly Val Trp Met Arg
            260                 265                 270
Asp Pro Lys Pro Thr Tyr Pro Tyr Thr Gln Glu Thr Thr Trp Arg Ile
        275                 280                 285
Asp Thr Val Gly Thr Asp Val Arg Gln Val Phe Glu Tyr Asp Leu Ile
    290                 295                 300
Ser Gln Phe Met Gln Gly Tyr Pro Ser Lys Val His Ile Leu Pro Arg
305                 310                 315                 320
Pro Leu Glu Ser Thr Gly Ala Val Val Tyr Ser Gly Ser Leu Tyr Phe
                325                 330                 335
Gln Gly Ala Glu Ser Arg Thr Val Ile Arg Tyr Glu Leu Asn Thr Glu
            340                 345                 350
Thr Val Lys Ala Glu Lys Glu Ile Pro Gly Ala Gly Tyr His Gly Gln
        355                 360                 365
Phe Pro Tyr Ser Trp Gly Gly Tyr Thr Asp Ile Asp Leu Ala Val Asp
    370                 375                 380
Glu Ala Gly Leu Trp Val Ile Tyr Ser Thr Asp Glu Ala Lys Gly Ala
385                 390                 395                 400
Ile Val Leu Ser Lys Leu Asn Pro Glu Asn Leu Glu Leu Glu Gln Thr
                405                 410                 415
Trp Glu Thr Asn Ile Arg Lys Gln Ser Val Ala Asn Ala Phe Ile Ile
            420                 425                 430
Cys Gly Thr Leu Tyr Thr Val Ser Ser Tyr Thr Ser Ala Asp Ala Thr
        435                 440                 445
Val Asn Phe Ala Tyr Asp Thr Gly Thr Gly Ile Ser Lys Thr Leu Thr
    450                 455                 460
Ile Pro Phe Lys Asn Arg Tyr Lys Tyr Ser Ser Met Ile Asp Tyr Asn
465                 470                 475                 480
Pro Leu Glu Lys Lys Leu Phe Ala Trp Asp Asn Leu Asn Met Val Thr
                485                 490                 495
Tyr Asp Ile Lys Leu Ser Lys Met
            500

<210> 8
<211> 1228
<212> DNA
<213> Homo sapiens

<400> 8
gatctccagt tcctagcata gtgcctggca cagtgcaggt tctcaatgag tttgcagagt      60
gaatggaaat ataaactaga aatatatcct tgttgaaatc agcacaccag tagtcctggt     120
gtaagtgtgt gtacgtgtgt gtgtgtgtgt gtgtgtgtgt aaaaccaggt ggagatatag     180
gaactattat tggggtatgg gtgcataaat tgggatgttc tttttaaaaa gaaactccaa     240
acagacttcc ggaaggttat tttctaagaa tcttgctggc agcgtgaagg caacccccct     300
gtgcacagcc ccacccagcc tcacgtggcc acctctgtct tcccccatga agggctggct     360
ccccagtata tataaacctc tctggagctc gggcatgagc cagcaaggcc acccatccag     420
gcacctctca gcacagcaga gctttccaga ggaagcctca ccaagcctct gcaatgaggt     480
tcttctgtgc acgttgctgc agctttgggc ctgagatgcc agctgtccag ctgctgcttc     540
tggcctgcct ggtgtgggat gtgggggcca ggacagctca gctcaggaag gccaatgacc     600
agagtggccg atgccagtat accttcagtg tggccagtcc caatgaatcc agctgcccag     660
agcagagcca ggccatgtca gtcatccata acttacagag agacagcagc acccaacgct     720
tagacctgga ggccaccaaa gctcgactca gctccctgga gagcctcctc caccaattga     780
ccttggacca ggctgccagg ccccaggaga cccaggaggg gctgcagagg gagctgggca     840
ccctgaggcg ggagcgggac cagctggaaa cccaaaccag agagttggag actgcctaca     900
gcaacctcct ccgagacaag tcagttctgg aggaagagaa gaagcgacta aggcaagaaa     960
atgagaatct ggccaggagg ttggaaagca gcagccagga ggtagcaagg ctgagaaggg    1020
gccagtgtcc ccagacccga gacactgctc gggctgtgcc accaggctcc agagaaggta    1080
agaatgcaga gtggggggac tctgagttca gcaggtgata tggctcgtag tgacctgcta    1140
caggcgctcc aggcctccct gcctgccctt tctcctagag actgcacagc tagcacaaga    1200
cagatgaatt aaggaaagca cagcgatc                                       1228

<210> 9

<400> 9
 000

<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 10
tggcctgcct ggtgtgggat                                                  20

<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 11
cagagagaca gcagcaccca                                                  20

<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 12
gacctggagg ccaccaaagc                                                  20

<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 13
tggaggaaga gaagaagcga                                                  20

<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 14
caggccatgt cagtcatcca                                                  20

<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> A synthetic sequence

<400> 15
ggaggaagag aagaagcgac                                                  20
