                         SEQUENCE LISTING

<110>  BEIJING MEIKANG GENO-IMMUNE BIOTECHNOLOGY CO., LTD.
 
<120>  UNIVERSAL SARS-COV-2 VACCINE AND PREPARATION METHOD THEREOF

<130>  20200911

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  198
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  S protein receptor binding domain

<400>  1

Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
1               5                   10                  15      


Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
            20                  25                  30          


Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
        35                  40                  45              


Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
    50                  55                  60                  


Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
65                  70                  75                  80  


Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
                85                  90                  95      


Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
            100                 105                 110         


Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
        115                 120                 125             


Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
    130                 135                 140                 


Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
145                 150                 155                 160 


Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
                165                 170                 175     


Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
            180                 185                 190         


Ala Thr Val Cys Gly Pro 
        195             


<210>  2
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  S protein signal peptide 

<400>  2

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro 
            20                  25  


<210>  3
<211>  228
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  fusion protein S 

<400>  3

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Phe Asp Ile Lys Leu Ile Ala Ile Pro Ser Thr Ser 
    210                 215                 220                 


Arg Glu Phe Arg 
225             


<210>  4
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>  
<223>   the amino terminal peptide fragment of the E protein

<400>  4

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      


Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val 
            20                  25                  


<210>  5
<211>  58
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  the carboxyl terminal peptide fragment of the M protein

<400>  5

Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu Ser Tyr Tyr Lys 
1               5                   10                  15      


Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly Phe Ala Ala Tyr 
            20                  25                  30          


Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr Asp His Ser Ser 
        35                  40                  45              


Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    50                  55              


<210>  6
<211>  315
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  fusion protein SEM

<400>  6

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Met 
    210                 215                 220                 


Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser Val 
225                 230                 235                 240 


Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Pro Lys Glu Ile 
                245                 250                 255     


Thr Val Ala Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser 
            260                 265                 270         


Gln Arg Val Ala Gly Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg 
        275                 280                 285             


Ile Gly Asn Tyr Lys Leu Asn Thr Asp His Ser Ser Ser Ser Asp Asn 
    290                 295                 300                 


Ile Ala Leu Leu Val Gln Pro Asn Asn Thr Ala 
305                 310                 315 


<210>  7
<211>  162
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  the helix-turn domain of the N protein

<400>  7

Pro Asn Asn Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys 
1               5                   10                  15      


Glu Asp Leu Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn 
            20                  25                  30          


Ser Ser Pro Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg 
        35                  40                  45              


Ile Arg Gly Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr 
    50                  55                  60                  


Phe Tyr Tyr Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala 
65                  70                  75                  80  


Asn Lys Asp Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr 
                85                  90                  95      


Pro Lys Asp His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile 
            100                 105                 110         


Val Leu Gln Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala 
        115                 120                 125             


Glu Gly Ser Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg 
    130                 135                 140                 


Ser Arg Asn Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr 
145                 150                 155                 160 


Ser Pro 
        


<210>  8
<211>  123
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  the partial peptide fragment of ORF1a poly-protease 

<400>  8

Pro Phe Val Asp Arg Gln Thr Ala Gln Ala Ala Gly Thr Asp Thr Thr 
1               5                   10                  15      


Ile Thr Val Asn Val Leu Ala Trp Leu Tyr Ala Ala Val Ile Asn Gly 
            20                  25                  30          


Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr Thr Leu Asn Asp Phe Asn 
        35                  40                  45              


Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro Leu Thr Gln Asp His Val 
    50                  55                  60                  


Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr Gly Ile Ala Val Leu Asp 
65                  70                  75                  80  


Met Cys Ala Ser Leu Lys Glu Leu Leu Gln Asn Gly Met Asn Gly Arg 
                85                  90                  95      


Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp Glu Phe Thr Pro Phe Asp 
            100                 105                 110         


Val Val Arg Gln Cys Ser Gly Val Thr Phe Gln 
        115                 120             


<210>  9
<211>  595
<212>  PRT
<213>  Artificial sequence

<220>  
<223>  fusion protein SEMNP

<400>  9

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Met 
    210                 215                 220                 


Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser Val 
225                 230                 235                 240 


Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Pro Lys Glu Ile 
                245                 250                 255     


Thr Val Ala Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser 
            260                 265                 270         


Gln Arg Val Ala Gly Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg 
        275                 280                 285             


Ile Gly Asn Tyr Lys Leu Asn Thr Asp His Ser Ser Ser Ser Asp Asn 
    290                 295                 300                 


Ile Ala Leu Leu Val Gln Pro Asn Asn Thr Ala Ser Trp Phe Thr Ala 
305                 310                 315                 320 


Leu Thr Gln His Gly Lys Glu Asp Leu Lys Phe Pro Arg Gly Gln Gly 
                325                 330                 335     


Val Pro Ile Asn Thr Asn Ser Ser Pro Asp Asp Gln Ile Gly Tyr Tyr 
            340                 345                 350         


Arg Arg Ala Thr Arg Arg Ile Arg Gly Gly Asp Gly Lys Met Lys Asp 
        355                 360                 365             


Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr Leu Gly Thr Gly Pro Glu Ala 
    370                 375                 380                 


Gly Leu Pro Tyr Gly Ala Asn Lys Asp Gly Ile Ile Trp Val Ala Thr 
385                 390                 395                 400 


Glu Gly Ala Leu Asn Thr Pro Lys Asp His Ile Gly Thr Arg Asn Pro 
                405                 410                 415     


Ala Asn Asn Ala Ala Ile Val Leu Gln Leu Pro Gln Gly Thr Thr Leu 
            420                 425                 430         


Pro Lys Gly Phe Tyr Ala Glu Gly Ser Arg Gly Gly Ser Gln Ala Ser 
        435                 440                 445             


Ser Arg Ser Ser Ser Arg Ser Arg Asn Ser Ser Arg Asn Ser Thr Pro 
    450                 455                 460                 


Gly Ser Ser Arg Gly Thr Ser Pro Pro Phe Val Asp Arg Gln Thr Ala 
465                 470                 475                 480 


Gln Ala Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp 
                485                 490                 495     


Leu Tyr Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe 
            500                 505                 510         


Thr Thr Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr 
        515                 520                 525             


Glu Pro Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala 
    530                 535                 540                 


Gln Thr Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu 
545                 550                 555                 560 


Leu Gln Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu 
                565                 570                 575     


Glu Asp Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val 
            580                 585                 590         


Thr Phe Gln 
        595 


<210>  10
<211>  687
<212>  DNA
<213>  Artificial sequence

<220>  
<223>  the coding gene of the fusion protein S

<400>  10
atgttcgtat tccttgtact cttgcctctc gttagcagtc agtgcgtaaa cctcactacg     60

aggacgcagt tgccccccaa cataacgaat ctgtgcccgt tcggagaggt tttcaacgcg    120

acccggtttg cgtctgtgta tgcgtggaac agaaagcgaa tctctaattg tgtcgccgat    180

tattctgttt tgtacaacag cgcatcattt tccaccttca aatgttatgg agtcagcccc    240

acaaagctga atgatctttg ctttacgaat gtctacgccg acagctttgt aatacgcggc    300

gacgaggtgc gacagatcgc ccccggacaa acagggaaga tcgcagacta caattataaa    360

ctgcctgatg acttcacggg ttgtgttatt gcgtggaata gtaataacct ggactctaaa    420

gttggcggca actacaatta tctgtatcgc ctgtttagaa agagtaatct caagcctttc    480

gagcgggaca tcagtaccga gatctaccag gccggctcta ccccgtgcaa cggtgtagaa    540

ggctttaatt gttactttcc cttgcagtcc tatggctttc agccaaccaa tggcgtgggc    600

tatcaaccgt accgggtagt tgtgctgtca ttcgaattcg atatcaagct tatcgcgata    660

ccgtcgacct cgagggaatt ccgataa                                        687


<210>  11
<211>  947
<212>  DNA
<213>  Artificial sequence

<220>  
<223>  the coding gene of the fusion protein SEM

<400>  11
atgttcgtat tccttgtact cttgcctctc gttagcagtc agtgcgtaaa cctcactacg     60

aggacgcagt tgccccccaa cataacgaat ctgtgcccgt tcggagaggt tttcaacgcg    120

acccggtttg cgtctgtgta tgcgtggaac agaaagcgaa tctctaattg tgtcgccgat    180

tattctgttt tgtacaacag cgcatcattt tccaccttca aatgttatgg agtcagcccc    240

acaaagctga atgatctttg ctttacgaat gtctacgccg acagctttgt aatacgcggc    300

gacgaggtgc gacagatcgc ccccggacaa acagggaaga tcgcagacta caattataaa    360

ctgcctgatg acttcacggg ttgtgttatt gcgtggaata gtaataacct ggactctaaa    420

gttggcggca actacaatta tctgtatcgc ctgtttagaa agagtaatct caagcctttc    480

gagcgggaca tcagtaccga gatctaccag gccggctcta ccccgtgcaa cggtgtagaa    540

ggctttaatt gttactttcc cttgcagtcc tatggctttc agccaaccaa tggcgtgggc    600

tatcaaccgt accgggtagt tgtgctgtca ttcgaactgt tgcacgcccc agcaacggtg    660

tgtgggccca tgtatagctt cgtgtctgaa gagaccggta ctctcattgt aaactccgtc    720

ctcctgttcc tcgcgttcgt agtgttcctc ctcgtgccta aagagataac agtggcaact    780

tctaggactc tctcctacta taaactcggt gcctcccaga gggtagcggg cgatagtggg    840

ttcgctgcct actccaggta tcgaatcgga aattataaac ttaatacgga tcacagttca    900

tcttccgata acatcgctct tcttgtacaa ccgaataaca ccgctag                  947


<210>  12
<211>  1788
<212>  DNA
<213>  Artificial sequence

<220>  
<223>  the coding gene of the fusion protein SEMNP

<400>  12
atgttcgtat tccttgtact cttgcctctc gttagcagtc agtgcgtaaa cctcactacg     60

aggacgcagt tgccccccaa cataacgaat ctgtgcccgt tcggagaggt tttcaacgcg    120

acccggtttg cgtctgtgta tgcgtggaac agaaagcgaa tctctaattg tgtcgccgat    180

tattctgttt tgtacaacag cgcatcattt tccaccttca aatgttatgg agtcagcccc    240

acaaagctga atgatctttg ctttacgaat gtctacgccg acagctttgt aatacgcggc    300

gacgaggtgc gacagatcgc ccccggacaa acagggaaga tcgcagacta caattataaa    360

ctgcctgatg acttcacggg ttgtgttatt gcgtggaata gtaataacct ggactctaaa    420

gttggcggca actacaatta tctgtatcgc ctgtttagaa agagtaatct caagcctttc    480

gagcgggaca tcagtaccga gatctaccag gccggctcta ccccgtgcaa cggtgtagaa    540

ggctttaatt gttactttcc cttgcagtcc tatggctttc agccaaccaa tggcgtgggc    600

tatcaaccgt accgggtagt tgtgctgtca ttcgaactgt tgcacgcccc agcaacggtg    660

tgtgggccca tgtatagctt cgtgtctgaa gagaccggta ctctcattgt aaactccgtc    720

ctcctgttcc tcgcgttcgt agtgttcctc ctcgtgccta aagagataac agtggcaact    780

tctaggactc tctcctacta taaactcggt gcctcccaga gggtagcggg cgatagtggg    840

ttcgctgcct actccaggta tcgaatcgga aattataaac ttaatacgga tcacagttca    900

tcttccgata acatcgctct tcttgtacaa ccgaataaca ccgctagctg gtttacggcc    960

ctcacgcagc acggtaagga agacctcaaa ttcccccgag gccagggtgt tccgataaac   1020

actaatagtt caccagacga ccaaattggg tattacagac gagctactcg acggattcga   1080

gggggagacg gtaagatgaa agacctgtca ccacggtggt atttttatta tttggggact   1140

ggtccggaag cgggcctgcc gtatggggca aataaggacg gtatcatctg ggtggctacg   1200

gaaggcgctc ttaacacacc caaggaccat ataggaaccc gaaatccagc taataatgcc   1260

gcaatagttt tgcaactccc ccaagggaca acacttccta aaggttttta tgccgaaggt   1320

agtcgcgggg gctcacaagc ctctagtcgg tcatcatcca ggtcccgcaa tagtagtcgc   1380

aactctactc cgggaagtag ccggggtaca tcccctccct ttgtggatag gcaaacggct   1440

caggcagcgg gtacggatac taccatcaca gtgaatgtgc ttgcatggct gtatgcagcg   1500

gttattaacg gggacaggtg gttcctgaac cggttcacca ctacattgaa tgatttcaat   1560

ctcgtagcca tgaagtataa ctacgagcca ttgactcaag atcatgtaga catcttgggg   1620

cccctgagcg cgcaaacggg aattgcagta ctggacatgt gcgcatcttt gaaagagttg   1680

ctgcagaacg gaatgaatgg aaggacgatt ctcggaagcg cattgctgga agacgaattc   1740

acaccatttg acgttgtccg acaatgctcc ggagtcacgt ttcagtga                1788


