               SEQUENCE LISTING

<110> Universita' degli Studi di Torino

<120> Anti-Morgana monoclonal antibody for the treatment of tumors

<130> E0136153-PC1871EC

<160> 16

<170> BiSSAP 1.3.6

<210> 1
<211> 26
<212> PRT
<213> Homo sapiens


<220> 
<223> Morgana epitope

<400> 1
Leu Ser Glu Leu Lys Pro Lys Phe Gln Glu His Ile Ile Gln Ala Pro 
1               5                   10                  15      
Lys Pro Val Glu Ala Ile Lys Arg Pro Ser 
            20                  25      

<210> 2
<211> 331
<212> PRT
<213> Homo sapiens


<220> 
<223> Morgana

<400> 2
Met Ala Leu Leu Cys Tyr Asn Arg Gly Cys Gly Gln Arg Phe Asp Pro 
1               5                   10                  15      
Glu Ala Asn Ser Asp Asp Ala Cys Thr Tyr His Pro Gly Val Pro Val 
            20                  25                  30          
Phe His Asp Ala Leu Lys Gly Trp Ser Cys Cys Lys Arg Arg Thr Thr 
        35                  40                  45              
Asp Phe Ser Asp Phe Leu Ser Ile Val Gly Cys Thr Lys Gly Arg His 
    50                  55                  60                  
Asn Ser Glu Lys Pro Pro Glu Pro Val Lys Pro Glu Val Lys Thr Thr 
65                  70                  75                  80  
Glu Lys Lys Glu Leu Ser Glu Leu Lys Pro Lys Phe Gln Glu His Ile 
                85                  90                  95      
Ile Gln Ala Pro Lys Pro Val Glu Ala Ile Lys Arg Pro Ser Pro Asp 
            100                 105                 110         
Glu Pro Met Thr Asn Leu Glu Leu Lys Ile Ser Ala Ser Leu Lys Gln 
        115                 120                 125             
Ala Leu Asp Lys Leu Lys Leu Ser Ser Gly Ser Glu Glu Asp Lys Lys 
    130                 135                 140                 
Glu Glu Asp Ser Asp Glu Ile Lys Ile Gly Thr Ser Cys Lys Asn Gly 
145                 150                 155                 160 
Gly Cys Ser Lys Thr Tyr Gln Gly Leu Gln Ser Leu Glu Glu Val Cys 
                165                 170                 175     
Val Tyr His Ser Gly Val Pro Ile Phe His Glu Gly Met Lys Tyr Trp 
            180                 185                 190         
Ser Cys Cys Arg Arg Lys Thr Ser Asp Phe Asn Thr Phe Leu Ala Gln 
        195                 200                 205             
Glu Gly Cys Thr Arg Gly Lys His Val Trp Thr Lys Lys Asp Ala Gly 
    210                 215                 220                 
Lys Lys Val Val Pro Cys Arg His Asp Trp His Gln Thr Gly Gly Glu 
225                 230                 235                 240 
Val Thr Ile Ser Val Tyr Ala Lys Asn Ser Leu Pro Glu Leu Ser Gln 
                245                 250                 255     
Val Glu Ala Asn Ser Thr Leu Leu Asn Val His Ile Val Phe Glu Gly 
            260                 265                 270         
Glu Lys Glu Phe His Gln Asn Val Lys Leu Trp Gly Val Ile Asp Val 
        275                 280                 285             
Lys Arg Ser Tyr Val Thr Met Thr Ala Thr Lys Ile Glu Ile Thr Met 
    290                 295                 300                 
Arg Lys Ala Glu Pro Met Gln Trp Ala Ser Leu Glu Leu Pro Thr Thr 
305                 310                 315                 320 
Lys Lys Gln Glu Lys Gln Lys Asp Ile Ala Asp 
                325                 330     

<210> 3
<211> 5
<212> PRT
<213> Mus musculus


<220> 
<223> CDRH1

<400> 3
Ser Tyr Trp Met His 
1               5   

<210> 4
<211> 17
<212> PRT
<213> Mus musculus


<220> 
<223> CDRH2

<400> 4
Gln Ile Asp Pro Ser Asp Asn Tyr Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      
Gly 
    

<210> 5
<211> 5
<212> PRT
<213> Mus musculus


<220> 
<223> CDRH3

<400> 5
Pro Tyr Gly Ser Tyr 
1               5   

<210> 6
<211> 16
<212> PRT
<213> Mus musculus


<220> 
<223> CDRL1

<400> 6
Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Ser Tyr Leu His 
1               5                   10                  15      



<210> 7
<211> 7
<212> PRT
<213> Mus musculus


<220> 
<223> CDRL2

<400> 7
Lys Val Ser Asn Arg Phe Ser 
1               5           

<210> 8
<211> 9
<212> PRT
<213> Mus musculus


<220> 
<223> CDRL3

<400> 8
Ser Gln Ser Thr His Val Pro Leu Thr 
1               5                   

<210> 9
<211> 114
<212> PRT
<213> Mus musculus


<220> 
<223> Heavy chain variable region

<400> 9
Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          
Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Gln Ile Asp Pro Ser Asp Asn Tyr Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Ser Pro Tyr Gly Ser Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         
Ser Ala 
        

<210> 10
<211> 438
<212> PRT
<213> Mus musculus


<220> 
<223> Heavy chain

<400> 10
Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          
Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Gln Ile Asp Pro Ser Asp Asn Tyr Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Ser Pro Tyr Gly Ser Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         
Ser Ala Ser Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly 
        115                 120                 125             
Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys 
    130                 135                 140                 
Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu 
145                 150                 155                 160 
Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr 
                165                 170                 175     
Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu 
            180                 185                 190         
Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp 
        195                 200                 205             
Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr 
    210                 215                 220                 
Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp 
225                 230                 235                 240 
Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 
                245                 250                 255     
Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp 
            260                 265                 270         
Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn 
        275                 280                 285             
Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp 
    290                 295                 300                 
Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro 
305                 310                 315                 320 
Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala 
                325                 330                 335     
Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp 
            340                 345                 350         
Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile 
        355                 360                 365             
Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn 
    370                 375                 380                 
Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys 
385                 390                 395                 400 
Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys 
                405                 410                 415     
Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu 
            420                 425                 430         
Ser His Ser Pro Gly Lys 
        435             

<210> 11
<211> 114
<212> PRT
<213> Mus musculus


<220> 
<223> Light chain variable region

<400> 11
Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          
Asn Gly Asn Ser Tyr Leu His Trp Tyr Leu Gln Asn Pro Gly Gln Ser 
        35                  40                  45              
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         
Arg Ala 
        

<210> 12
<211> 219
<212> PRT
<213> Mus musculus


<220> 
<223> Light chain

<400> 12
Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          
Asn Gly Asn Ser Tyr Leu His Trp Tyr Leu Gln Asn Pro Gly Gln Ser 
        35                  40                  45              
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      
Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         
Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             
Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 
Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         
Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             
Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 

<210> 13
<211> 1374
<212> DNA
<213> Mus musculus


<220> 
<223> Heavy chain (includes signal sequence and stop codon)

<400> 13
atgggatgga gctgtatcat cctcttcttg gtatcaacag ctacaggtgt ccactcccag      60

gtccaactgc agcagcctgg ggctgagctt gtgaagcctg gggcttcagt gaagctgtcc     120

tgcaaggctt ctggctacac cttcacctcc tactggatgc actgggtgaa acagaggcct     180

ggacaaggcc ttgagtggat cggacagatt gatccttctg ataattatac tagctacaat     240

caaaaattca agggcaaggc cacattgact gtagacacat cctccagcac agcctacatg     300

caactcagca gcctgacatc tgaggattct gcggtctatt actgtgcaag tccgtatggt     360

agttactggg gccaagggac tctggtcact gtctctgcat ccaaaacgac acccccatct     420

gtctatccac tggcccctgg atctgctgcc caaactaact ccatggtgac cctgggatgc     480

ctggtcaagg gctatttccc tgagccagtg acagtgacct ggaactctgg atccctgtcc     540

agcggtgtgc acaccttccc agctgtcctg cagtctgacc tctacactct gagcagctca     600

gtgactgtcc cctccagcac ctggcccagc gagaccgtca cctgcaacgt tgcccacccg     660

gccagcagca ccaaggtgga caagaaaatt gtgcccaggg attgtggttg taagccttgc     720

atatgtacag tcccagaagt atcatctgtc ttcatcttcc ccccaaagcc caaggatgtg     780

ctcaccatta ctctgactcc taaggtcacg tgtgttgtgg tagacatcag caaggatgat     840

cccgaggtcc agttcagctg gtttgtagat gatgtggagg tgcacacagc tcagacgcaa     900

ccccgggagg agcagttcaa cagcactttc cgctcagtca gtgaacttcc catcatgcac     960

caggactggc tcaatggcaa ggagttcaaa tgcagggtca acagtgcagc tttccctgcc    1020

cccatcgaga aaaccatctc caaaaccaaa ggcagaccga aggctccaca ggtgtacacc    1080

attccacctc ccaaggagca gatggccaag gataaagtca gtctgacctg catgataaca    1140

gacttcttcc ctgaagacat tactgtggag tggcagtgga atgggcagcc agcggagaac    1200

tacaagaaca ctcagcccat catggacaca gatggctctt acttcgtcta cagcaagctc    1260

aatgtgcaga agagcaactg ggaggcagga aatactttca cctgctctgt gttacatgag    1320

ggcctgcaca accaccatac tgagaagagc ctctcccact ctcctggtaa atga          1374


<210> 14
<211> 457
<212> PRT
<213> Mus musculus


<220> 
<223> Heavy chain (includes signal peptide)

<400> 14
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ser Thr Ala Thr Gly 
1               5                   10                  15      
Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          
Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              
Thr Ser Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  
Glu Trp Ile Gly Gln Ile Asp Pro Ser Asp Asn Tyr Thr Ser Tyr Asn 
65                  70                  75                  80  
Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      
Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         
Tyr Tyr Cys Ala Ser Pro Tyr Gly Ser Tyr Trp Gly Gln Gly Thr Leu 
        115                 120                 125             
Val Thr Val Ser Ala Ser Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
    130                 135                 140                 
Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys 
145                 150                 155                 160 
Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
                165                 170                 175     
Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
            180                 185                 190         
Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp 
        195                 200                 205             
Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
    210                 215                 220                 
Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys 
225                 230                 235                 240 
Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys 
                245                 250                 255     
Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val 
            260                 265                 270         
Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe 
        275                 280                 285             
Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu 
    290                 295                 300                 
Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His 
305                 310                 315                 320 
Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala 
                325                 330                 335     
Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg 
            340                 345                 350         
Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met 
        355                 360                 365             
Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro 
    370                 375                 380                 
Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn 
385                 390                 395                 400 
Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val 
                405                 410                 415     
Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr 
            420                 425                 430         
Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu 
        435                 440                 445             
Lys Ser Leu Ser His Ser Pro Gly Lys 
    450                 455         

<210> 15
<211> 717
<212> DNA
<213> Mus musculus


<220> 
<223> Light chain (includes signal sequence and stop codon)

<400> 15
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgat      60

gttgtgatga cccaaactcc actctccctg cctgtcagtc ttggtgatca agcctccatc     120

tcttgcagat ctagtcagag ccttgtacac agtaatggaa actcctattt acattggtac     180

ctgcagaacc caggccagtc tccaaagctc ctgatctaca aagtttccaa ccgattttct     240

ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact caagatcagc     300

agagtggagg ctgaggatct gggagtttat ttctgctctc aaagtacaca tgttccgctc     360

acgttcggtg ctgggaccaa gctggagctg aaacgggctg atgctgcacc aactgtatcc     420

atcttcccac catccagtga gcagttaaca tctggaggtg cctcagtcgt gtgcttcttg     480

aacaacttct accccaaaga catcaatgtc aagtggaaga ttgatggcag tgaacgacaa     540

aatggcgtcc tgaacagttg gactgatcag gacagcaaag acagcaccta cagcatgagc     600

agcaccctca cgttgaccaa ggacgagtat gaacgacata acagctatac ctgtgaggcc     660

actcacaaga catcaacttc acccattgtc aagagcttca acaggaatga gtgttag        717


<210> 16
<211> 238
<212> PRT
<213> Mus musculus


<220> 
<223> Light chain (includes signal peptide)

<400> 16
Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      
Ser Ser Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          
Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu 
        35                  40                  45              
Val His Ser Asn Gly Asn Ser Tyr Leu His Trp Tyr Leu Gln Asn Pro 
    50                  55                  60                  
Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  
Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      
Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys 
            100                 105                 110         
Ser Gln Ser Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
        115                 120                 125             
Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
    130                 135                 140                 
Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu 
145                 150                 155                 160 
Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly 
                165                 170                 175     
Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser 
            180                 185                 190         
Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp 
        195                 200                 205             
Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr 
    210                 215                 220                 
Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230                 235             

