                         SEQUENCE LISTING

<110>  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
       KOHN, DONALD B
       PAUL, AYOUB
 
<120>  SARS-COV-2 SPIKE GLYCOPROTEIN FOR VIRUS GENERATION AND 
       PSEUDOTYPING

<130>  UCLAP229WO

<140>  PCT/US2021/049312
<141>  2021-09-07

<150>  US 63/076,227
<151>  2020-09-09

<150>  US 63/075,703
<151>  2020-09-08

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  8202
<212>  DNA
<213>  Artificial

<220>
<223>  Lentiviral construct encoding modified SARS-CoV-2 spike protein

<400>  1
ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc       60

caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg      120

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc      180

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca      240

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg      300

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg      360

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt      420

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca      480

tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg      540

tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact      600

ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag      660

ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata      720

gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat      780

cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta      840

tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca      900

gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt      960

tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta     1020

tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag     1080

caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat     1140

attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag     1200

gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct     1260

tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag     1320

aattctgaca ctatgttcgt tttccttgtt ctgttgcctc tcgttagtag ccaatgcgtc     1380

aaccttacta ctagaaccca gctccctcca gcatatacca actctttcac caggggcgta     1440

tattacccgg acaaagtgtt ccgctcaagt gtgctgcatt ctacgcagga ccttttcttg     1500

ccctttttca gtaatgttac ttggtttcat gctatccatg tgtctggaac taacggaacc     1560

aagcgctttg acaaccccgt cctccctttc aacgatggcg tgtacttcgc ttccacggaa     1620

aagtcaaaca taattcgcgg ctggatcttt ggtacaacac tcgactcaaa gacgcagagc     1680

ctgctgatcg ttaataacgc tacaaatgtt gtgataaagg tgtgtgaatt tcagttctgc     1740

aatgatccct tcctgggtgt gtactaccat aagaataaca agagctggat ggaatccgaa     1800

tttagggttt acagttccgc taacaactgc acattcgaat acgtaagcca gccatttctt     1860

atggatcttg agggcaagca aggaaacttc aagaacttga gggagttcgt gttcaaaaat     1920

atcgacggct attttaagat atatagcaag cacactccaa taaacttggt gcgcgacctg     1980

ccccagggat tctctgctct ggagcccctg gtggatctgc ccattggaat aaacataact     2040

cgctttcaaa cactgctcgc cctgcatcgc agttacctca cccctggtga tagtagttca     2100

ggatggacag caggagccgc cgcatactac gtcggctacc tgcagcctag gaccttcttg     2160

ctgaagtaca acgagaacgg tacaataact gacgctgtgg actgcgctct ggaccctctg     2220

tccgagacga agtgcaccct gaagagcttt actgttgaaa aaggcattta ccaaaccagc     2280

aacttccgcg tccagccaac cgagagcatc gtcagatttc ccaacattac aaatctgtgt     2340

cccttcggcg aggtgttcaa cgccacacgc ttcgcttcag tgtacgcatg gaaccgcaag     2400

cgcatatcta actgcgtcgc ggattattct gtcctctaca actccgcctc tttctccacc     2460

ttcaagtgct acggagtgtc accgactaag ctgaacgatc tctgctttac caacgtctac     2520

gcggactcct tcgtgataag aggtgatgaa gtgagacaaa tagccccagg tcagactggt     2580

aagatcgcag attacaacta caaattgcct gatgatttca ctggttgcgt tatcgcgtgg     2640

aactctaata acctcgattc taaggtcggt ggtaactaca attacctgta ccgcttgttt     2700

aggaagtcaa acctgaagcc tttcgagagg gatatttcaa ccgaaatcta tcaagcgggt     2760

tcaacaccgt gtaacggtgt ggaaggattt aactgctact tccccctgca gtcttacgga     2820

ttccagccaa ccaatggcgt gggttaccaa ccttatcgcg tggtggttct gagtttcgaa     2880

ctgttgcacg ctcccgccac ggtatgcggt cccaagaaga gcactaactt ggtgaagaat     2940

aagtgcgtga atttcaattt caatggcctc actggaactg gagtgctgac cgaatccaat     3000

aagaagttct tgcccttcca gcagttcgga agagacattg ctgacacaac cgacgcggtg     3060

cgcgatcctc agactctgga gatattggac attacaccat gttctttcgg cggtgtgtct     3120

gtcattactc cgggcacgaa tactagcaac caggtagccg tgctgtacca aggcgtgaat     3180

tgcacagagg ttcccgtcgc aattcacgct gaccagctga cccccacgtg gagggtttac     3240

agcactggta gtaacgtctt ccagacgaga gccggttgct tgatcggagc ggaacatgtg     3300

aataactcct acgagtgcga catccccatc ggagccggta tatgcgcctc ttatcagaca     3360

caaactaact cacccaggag agcccgcagt gtggcttctc aaagcattat agcatacact     3420

atgtctcttg gtgccgaaaa ttccgtggcc tattctaaca attcaatcgc catcccaacc     3480

aacttcacaa ttagcgtgac taccgaaata ctgcctgtga gcatgacgaa aaccagcgta     3540

gactgcacta tgtatatctg tggagactcc actgagtgct ccaaccttct cctgcagtac     3600

ggtagcttct gtacccaatt gaaccgcgcc cttacaggca tcgctgttga gcaagataag     3660

aatacccagg aagtttttgc ccaggttaag cagatataca aaacaccgcc cattaaggac     3720

ttcggaggct tcaacttctc tcagatactg cctgacccct ccaagccatc aaaacgcagc     3780

ttcattgagg acctcttgtt caacaaagtg actctggctg atgctggctt cattaagcag     3840

tacggagatt gcctgggaga tattgctgcc agggacctca tctgcgccca gaagtttaat     3900

ggcctgacag tcttgccccc acttctgaca gacgagatga ttgctcagta cacatctgcc     3960

ctcctcgctg gcaccataac atccggatgg acatttggtg ctggtgctgc cctccagatt     4020

cccttcgcaa tgcagatggc gtatcgcttt aacggcatcg gtgtcacaca aaacgtgttg     4080

tatgagaacc aaaagctcat cgctaaccag tttaattctg ctattggtaa gattcaggac     4140

agcctgtcat caaccgcgtc tgcccttggt aagttgcagg acgtggtgaa ccagaatgct     4200

caggctttga atactctggt gaagcaactc tcttcaaatt tcggcgctat ctcttctgtg     4260

ttgaacgaca tcctgagtcg ccttgataag gtggaagctg aagttcaaat tgatagattg     4320

attactggca ggctccagtc tttgcagacc tacgttacac agcagctgat tagggcggct     4380

gaaattagag cttccgccaa tctggctgca accaagatgt ccgaatgcgt cctgggtcag     4440

tcaaagcgcg ttgacttttg tggtaaaggc taccacctca tgtcatttcc ccagtcagca     4500

cctcacggag tagtgttcct ccacgtcacc tacgttccag cacaggaaaa gaattttacc     4560

actgcgccgg caatctgtca cgacggtaag gcacacttcc cccgcgaggg cgtattcgtg     4620

tctaacggaa ctcattggtt cgtcacacag agaaacttct atgagcctca gatcattacc     4680

accgacaata catttgtgtc cggtaactgc gacgttgtga ttggaatcgt caacaacact     4740

gtgtacgatc cacttcagcc agaactggat agcttcaagg aagaattgga caaatatttc     4800

aaaaatcaca cttcacccga tgtggacctg ggtgacatta gtggtatcaa tgcgtccgtg     4860

gtcaatattc aaaaagagat tgacaggctc aacgaagtgg ccaagaacct gaacgaaagt     4920

cttatcgatc tgcaagaatt gggaaagtat gagcagtaca tcaagtggcc gtggtacatt     4980

tggttgggtt ttatcgccgg tctgatcgcc atcgttatgg ttaccattat gctttgctgc     5040

atgacgagca atgggtcttt gcagtgcaga atatgcatct aatgaaagct tatcgatacc     5100

gtcgacctcg agggcccaga tctgaattca ccccaccagt gcaggctgcc tatcagaaag     5160

tggtggctgg tgtggctaat gccctggccc acaagtatca ctaagctcgc tttcttgctg     5220

tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt     5280

atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt cattgcaatg     5340

atgtatttaa attatttctg aatattttac taaaaaggga atgtgggagg tcagtgcatt     5400

taaaacataa agaaatgaag agctagttca aaccttggga aaatacacta tatcttaaac     5460

tccatgaaag aaggtgaggc tgcaaacagc taatgcacat tggcaacagc ccctgatgcc     5520

tatgccttat tcatccctca gaaaaggatt caagtagagg cttgatttgg aggttaaagt     5580

tttgctatgc tgtattttac attacttatt gttttagctg tcctcatgaa tgtcttttca     5640

ctacccattt gcttatcctg catctctcag ccttgactcc actcagttct cttgcttaga     5700

gataccacct ttcccctgaa gtgttccttc catgttttac ggcgagatgg tttctcctcg     5760

cctggccact cagccttagt tgtctctgtt gtcttataga ggtctacttg aagaaggaaa     5820

aacagggggc atggtttgac tgtcctgtga gcccttcttc cctgcctccc ccactcacag     5880

tgacccggaa tccctcgaca tggcagtcta gaattcttga agacgaaagg gcctcgtgat     5940

acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac     6000

ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat     6060

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag     6120

tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc     6180

tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc     6240

acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc     6300

cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc     6360

ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt     6420

ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt     6480

atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat     6540

cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct     6600

tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat     6660

gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc     6720

ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg     6780

ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc     6840

tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta     6900

cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc     6960

ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga     7020

tttaaaactt catttttaat ttaaaaggat ctaggctgct gcttgcaaac aaaaaaacca     7080

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     7140

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     7200

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     7260

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     7320

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     7380

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     7440

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     7500

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     7560

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     7620

gccagcaacg gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt     7680

ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct     7740

tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg     7800

ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc     7860

catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt     7920

ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg     7980

tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa     8040

gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcggggagct ttttgcaaaa     8100

gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc     8160

ctcggcctct gcataaataa aaaaaattag tcagccatga gc                        8202


<210>  2
<211>  2654
<212>  PRT
<213>  Artificial

<220>
<223>  Proteins expressed by lentiviral construct encoding modified 
       SARS-CoV-2 spike

<400>  2

Leu Ala His Cys Ile Arg Cys Ile His Ile Ile Ile Cys Thr Phe Ile 
1               5                   10                  15      


Leu Ala His Val Gln His Tyr Arg His Val Asp Ile Asp Tyr Leu Val 
            20                  25                  30          


Ile Asn Ser Asn Gln Leu Arg Gly His Phe Ile Ala His Ile Trp Ser 
        35                  40                  45              


Ser Ala Leu His Asn Leu Arg Met Ala Arg Leu Ala Asp Arg Pro Thr 
    50                  55                  60                  


Thr Pro Ala His Arg Gln Arg Met Phe Pro Arg Gln Gly Leu Ser Ile 
65                  70                  75                  80  


Asp Val Asn Gly Trp Ser Ile Tyr Gly Lys Leu Pro Thr Trp Gln Tyr 
                85                  90                  95      


Ile Lys Cys Ile Ile Cys Gln Val Arg Pro Leu Leu Thr Ser Met Thr 
            100                 105                 110         


Val Asn Gly Pro Pro Gly Ile Met Pro Ser Thr Pro Tyr Gly Thr Phe 
        115                 120                 125             


Leu Leu Gly Ser Thr Ser Thr Tyr Ser Ser Leu Leu Pro Trp Cys Gly 
    130                 135                 140                 


Phe Gly Ser Thr Ser Met Gly Val Asp Ser Gly Leu Thr His Gly Asp 
145                 150                 155                 160 


Phe Gln Val Ser Thr Pro Leu Thr Ser Met Gly Val Cys Phe Gly Thr 
                165                 170                 175     


Lys Ile Asn Gly Thr Phe Gln Asn Val Val Thr Thr Pro Pro His Arg 
            180                 185                 190         


Lys Trp Ala Val Gly Val Tyr Gly Gly Arg Ser Ile Ala Glu Leu Val 
        195                 200                 205             


Thr Val Arg Ser Pro Gly Asp Ala Ile His Ala Val Leu Thr Ser Ile 
    210                 215                 220                 


Glu Asp Thr Gly Thr Asp Pro Ala Ser Pro Arg Ser Leu His Val Val 
225                 230                 235                 240 


Pro Ser Ser Asp Pro Glu Asn Phe Arg Val Ser Leu Trp Asp Pro Cys 
                245                 250                 255     


Phe Leu Ser Pro Ser Phe Leu Trp Leu Ser Ser Cys His Arg Lys Gly 
            260                 265                 270         


Arg Ser Asn Arg Val His Ile Leu Thr Lys Ser Gly Phe Cys Ile Cys 
        275                 280                 285             


Asn Phe Lys Lys Cys Phe Leu Leu Leu Ile Tyr Phe Phe Val Tyr Leu 
    290                 295                 300                 


Ile Ser Asn Thr Phe Pro Asn Leu Phe Leu Ser Gly Gln Tyr Asn Val 
305                 310                 315                 320 


Ser Cys Leu Phe Ala Pro Phe Arg Ile Thr Val Ile Ile Ser Gly Leu 
                325                 330                 335     


Arg Gln Gln Tyr Phe Cys Ile Ile Phe Leu His Ile Asn Cys Asn Cys 
            340                 345                 350         


Lys Arg Phe His Ile Ala Asn Ser Ser Tyr Asn Pro Ala Thr Ile Leu 
        355                 360                 365             


Leu Leu Phe Tyr Gly Trp Asp Lys Ala Gly Leu Phe Val Gln Ala Arg 
    370                 375                 380                 


Pro Phe Cys Ser Cys Ser Tyr Leu Leu Ser Ser Ser His Ser Ser Trp 
385                 390                 395                 400 


Ala Thr Cys Trp Ser Val Cys Trp Pro Ile Thr Leu Ala Lys Asn Ser 
                405                 410                 415     


Asp Thr Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln 
            420                 425                 430         


Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
        435                 440                 445             


Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser 
    450                 455                 460                 


Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val 
465                 470                 475                 480 


Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg 
                485                 490                 495     


Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser 
            500                 505                 510         


Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu 
        515                 520                 525             


Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val 
    530                 535                 540                 


Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly 
545                 550                 555                 560 


Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg 
                565                 570                 575     


Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro 
            580                 585                 590         


Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg 
        595                 600                 605             


Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys 
    610                 615                 620                 


His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala 
625                 630                 635                 640 


Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe 
                645                 650                 655     


Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser 
            660                 665                 670         


Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
        675                 680                 685             


Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr 
    690                 695                 700                 


Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr 
705                 710                 715                 720 


Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe 
                725                 730                 735     


Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
            740                 745                 750         


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
        755                 760                 765             


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
    770                 775                 780                 


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
785                 790                 795                 800 


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
                805                 810                 815     


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
            820                 825                 830         


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
        835                 840                 845             


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
    850                 855                 860                 


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
865                 870                 875                 880 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
                885                 890                 895     


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            900                 905                 910         


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
        915                 920                 925             


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
    930                 935                 940                 


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
945                 950                 955                 960 


Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
                965                 970                 975     


Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
            980                 985                 990         


Ala Val Arg Asp Pro Gln Thr Leu  Glu Ile Leu Asp Ile  Thr Pro Cys 
        995                 1000                 1005             


Ser Phe  Gly Gly Val Ser Val  Ile Thr Pro Gly Thr  Asn Thr Ser 
    1010                 1015                 1020             


Asn Gln  Val Ala Val Leu Tyr  Gln Gly Val Asn Cys  Thr Glu Val 
    1025                 1030                 1035             


Pro Val  Ala Ile His Ala Asp  Gln Leu Thr Pro Thr  Trp Arg Val 
    1040                 1045                 1050             


Tyr Ser  Thr Gly Ser Asn Val  Phe Gln Thr Arg Ala  Gly Cys Leu 
    1055                 1060                 1065             


Ile Gly  Ala Glu His Val Asn  Asn Ser Tyr Glu Cys  Asp Ile Pro 
    1070                 1075                 1080             


Ile Gly  Ala Gly Ile Cys Ala  Ser Tyr Gln Thr Gln  Thr Asn Ser 
    1085                 1090                 1095             


Pro Arg  Arg Ala Arg Ser Val  Ala Ser Gln Ser Ile  Ile Ala Tyr 
    1100                 1105                 1110             


Thr Met  Ser Leu Gly Ala Glu  Asn Ser Val Ala Tyr  Ser Asn Asn 
    1115                 1120                 1125             


Ser Ile  Ala Ile Pro Thr Asn  Phe Thr Ile Ser Val  Thr Thr Glu 
    1130                 1135                 1140             


Ile Leu  Pro Val Ser Met Thr  Lys Thr Ser Val Asp  Cys Thr Met 
    1145                 1150                 1155             


Tyr Ile  Cys Gly Asp Ser Thr  Glu Cys Ser Asn Leu  Leu Leu Gln 
    1160                 1165                 1170             


Tyr Gly  Ser Phe Cys Thr Gln  Leu Asn Arg Ala Leu  Thr Gly Ile 
    1175                 1180                 1185             


Ala Val  Glu Gln Asp Lys Asn  Thr Gln Glu Val Phe  Ala Gln Val 
    1190                 1195                 1200             


Lys Gln  Ile Tyr Lys Thr Pro  Pro Ile Lys Asp Phe  Gly Gly Phe 
    1205                 1210                 1215             


Asn Phe  Ser Gln Ile Leu Pro  Asp Pro Ser Lys Pro  Ser Lys Arg 
    1220                 1225                 1230             


Ser Phe  Ile Glu Asp Leu Leu  Phe Asn Lys Val Thr  Leu Ala Asp 
    1235                 1240                 1245             


Ala Gly  Phe Ile Lys Gln Tyr  Gly Asp Cys Leu Gly  Asp Ile Ala 
    1250                 1255                 1260             


Ala Arg  Asp Leu Ile Cys Ala  Gln Lys Phe Asn Gly  Leu Thr Val 
    1265                 1270                 1275             


Leu Pro  Pro Leu Leu Thr Asp  Glu Met Ile Ala Gln  Tyr Thr Ser 
    1280                 1285                 1290             


Ala Leu  Leu Ala Gly Thr Ile  Thr Ser Gly Trp Thr  Phe Gly Ala 
    1295                 1300                 1305             


Gly Ala  Ala Leu Gln Ile Pro  Phe Ala Met Gln Met  Ala Tyr Arg 
    1310                 1315                 1320             


Phe Asn  Gly Ile Gly Val Thr  Gln Asn Val Leu Tyr  Glu Asn Gln 
    1325                 1330                 1335             


Lys Leu  Ile Ala Asn Gln Phe  Asn Ser Ala Ile Gly  Lys Ile Gln 
    1340                 1345                 1350             


Asp Ser  Leu Ser Ser Thr Ala  Ser Ala Leu Gly Lys  Leu Gln Asp 
    1355                 1360                 1365             


Val Val  Asn Gln Asn Ala Gln  Ala Leu Asn Thr Leu  Val Lys Gln 
    1370                 1375                 1380             


Leu Ser  Ser Asn Phe Gly Ala  Ile Ser Ser Val Leu  Asn Asp Ile 
    1385                 1390                 1395             


Leu Ser  Arg Leu Asp Lys Val  Glu Ala Glu Val Gln  Ile Asp Arg 
    1400                 1405                 1410             


Leu Ile  Thr Gly Arg Leu Gln  Ser Leu Gln Thr Tyr  Val Thr Gln 
    1415                 1420                 1425             


Gln Leu  Ile Arg Ala Ala Glu  Ile Arg Ala Ser Ala  Asn Leu Ala 
    1430                 1435                 1440             


Ala Thr  Lys Met Ser Glu Cys  Val Leu Gly Gln Ser  Lys Arg Val 
    1445                 1450                 1455             


Asp Phe  Cys Gly Lys Gly Tyr  His Leu Met Ser Phe  Pro Gln Ser 
    1460                 1465                 1470             


Ala Pro  His Gly Val Val Phe  Leu His Val Thr Tyr  Val Pro Ala 
    1475                 1480                 1485             


Gln Glu  Lys Asn Phe Thr Thr  Ala Pro Ala Ile Cys  His Asp Gly 
    1490                 1495                 1500             


Lys Ala  His Phe Pro Arg Glu  Gly Val Phe Val Ser  Asn Gly Thr 
    1505                 1510                 1515             


His Trp  Phe Val Thr Gln Arg  Asn Phe Tyr Glu Pro  Gln Ile Ile 
    1520                 1525                 1530             


Thr Thr  Asp Asn Thr Phe Val  Ser Gly Asn Cys Asp  Val Val Ile 
    1535                 1540                 1545             


Gly Ile  Val Asn Asn Thr Val  Tyr Asp Pro Leu Gln  Pro Glu Leu 
    1550                 1555                 1560             


Asp Ser  Phe Lys Glu Glu Leu  Asp Lys Tyr Phe Lys  Asn His Thr 
    1565                 1570                 1575             


Ser Pro  Asp Val Asp Leu Gly  Asp Ile Ser Gly Ile  Asn Ala Ser 
    1580                 1585                 1590             


Val Val  Asn Ile Gln Lys Glu  Ile Asp Arg Leu Asn  Glu Val Ala 
    1595                 1600                 1605             


Lys Asn  Leu Asn Glu Ser Leu  Ile Asp Leu Gln Glu  Leu Gly Lys 
    1610                 1615                 1620             


Tyr Glu  Gln Tyr Ile Lys Trp  Pro Trp Tyr Ile Trp  Leu Gly Phe 
    1625                 1630                 1635             


Ile Ala  Gly Leu Ile Ala Ile  Val Met Val Thr Ile  Met Leu Cys 
    1640                 1645                 1650             


Cys Met  Thr Ser Asn Gly Ser  Leu Gln Cys Arg Ile  Cys Ile Lys 
    1655                 1660                 1665             


Leu Ile  Asp Thr Val Asp Leu  Glu Gly Pro Asp Leu  Asn Ser Pro 
    1670                 1675                 1680             


His Gln  Cys Arg Leu Pro Ile  Arg Lys Trp Trp Leu  Val Trp Leu 
    1685                 1690                 1695             


Met Pro  Trp Pro Thr Ser Ile  Thr Lys Leu Ala Phe  Leu Leu Ser 
    1700                 1705                 1710             


Asn Phe  Tyr Arg Phe Leu Cys  Ser Leu Ser Pro Thr  Thr Lys Leu 
    1715                 1720                 1725             


Gly Asp  Ile Met Lys Gly Leu  Glu His Leu Asp Ser  Ala Lys Thr 
    1730                 1735                 1740             


Phe Ile  Phe Ile Ala Met Met  Tyr Leu Asn Tyr Phe  Ile Phe Tyr 
    1745                 1750                 1755             


Lys Gly  Asn Val Gly Gly Gln  Cys Ile Asn Ile Lys  Lys Arg Ala 
    1760                 1765                 1770             


Ser Ser  Asn Leu Gly Lys Ile  His Tyr Ile Leu Asn  Ser Met Lys 
    1775                 1780                 1785             


Glu Gly  Glu Ala Ala Asn Ser  Cys Thr Leu Ala Thr  Ala Pro Asp 
    1790                 1795                 1800             


Ala Tyr  Ala Leu Phe Ile Pro  Gln Lys Arg Ile Gln  Val Glu Ala 
    1805                 1810                 1815             


Phe Gly  Gly Ser Phe Ala Met  Leu Tyr Phe Thr Leu  Leu Ile Val 
    1820                 1825                 1830             


Leu Ala  Val Leu Met Asn Val  Phe Ser Leu Pro Ile  Cys Leu Ser 
    1835                 1840                 1845             


Cys Ile  Ser Gln Pro Leu His  Ser Val Leu Leu Leu  Arg Asp Thr 
    1850                 1855                 1860             


Thr Phe  Pro Leu Lys Cys Ser  Phe His Val Leu Arg  Arg Asp Gly 
    1865                 1870                 1875             


Phe Ser  Ser Pro Gly His Ser  Ala Leu Val Val Ser  Val Val Leu 
    1880                 1885                 1890             


Arg Ser  Thr Arg Arg Lys Asn  Arg Gly His Gly Leu  Thr Val Leu 
    1895                 1900                 1905             


Ala Leu  Leu Pro Cys Leu Pro  His Ser Gln Pro Gly  Ile Pro Arg 
    1910                 1915                 1920             


His Gly  Ser Leu Glu Phe Leu  Lys Thr Lys Gly Pro  Arg Asp Thr 
    1925                 1930                 1935             


Pro Ile  Phe Ile Gly Cys His  Asp Asn Asn Gly Phe  Leu Asp Val 
    1940                 1945                 1950             


Arg Trp  His Phe Ser Gly Lys  Cys Ala Arg Asn Pro  Tyr Leu Phe 
    1955                 1960                 1965             


Ile Phe  Leu Asn Thr Phe Lys  Tyr Val Ser Ala His  Glu Thr Ile 
    1970                 1975                 1980             


Thr Leu  Ile Asn Ala Ser Ile  Ile Leu Lys Lys Glu  Glu Tyr Glu 
    1985                 1990                 1995             


Tyr Ser  Thr Phe Pro Cys Arg  Pro Tyr Ser Leu Phe  Cys Gly Ile 
    2000                 2005                 2010             


Leu Pro  Ser Cys Phe Cys Ser  Pro Arg Asn Ala Gly  Glu Ser Lys 
    2015                 2020                 2025             


Arg Cys  Arg Ser Val Gly Cys  Thr Ser Gly Leu His  Arg Thr Gly 
    2030                 2035                 2040             


Ser Gln  Gln Arg Asp Pro Glu  Phe Ser Pro Arg Arg  Thr Phe Ser 
    2045                 2050                 2055             


Asn Asp  Glu His Phe Ser Ser  Ala Met Trp Arg Gly  Ile Ile Pro 
    2060                 2065                 2070             


Cys Arg  Arg Ala Arg Ala Thr  Arg Ser Pro His Thr  Leu Phe Ser 
    2075                 2080                 2085             


Glu Leu  Gly Val Leu Thr Ser  His Arg Lys Ala Ser  Tyr Gly Trp 
    2090                 2095                 2100             


His Asp  Ser Lys Arg Ile Met  Gln Cys Cys His Asn  His Glu His 
    2105                 2110                 2115             


Cys Gly  Gln Leu Thr Ser Asp  Asn Asp Arg Arg Thr  Glu Gly Ala 
    2120                 2125                 2130             


Asn Arg  Phe Phe Ala Gln His  Gly Gly Ser Cys Asn  Ser Pro Ser 
    2135                 2140                 2145             


Leu Gly  Thr Gly Ala Glu Ser  His Thr Lys Arg Arg  Ala His His 
    2150                 2155                 2160             


Asp Ala  Cys Ser Asn Gly Asn  Asn Val Ala Gln Thr  Ile Asn Trp 
    2165                 2170                 2175             


Arg Thr  Thr Tyr Ser Ser Phe  Pro Ala Thr Ile Asn  Arg Leu Asp 
    2180                 2185                 2190             


Gly Gly  Gly Ser Cys Arg Thr  Thr Ser Ala Leu Gly  Pro Ser Gly 
    2195                 2200                 2205             


Trp Leu  Val Tyr Cys Ile Trp  Ser Arg Ala Trp Val  Ser Arg Tyr 
    2210                 2215                 2220             


His Cys  Ser Thr Gly Ala Arg  Trp Ala Leu Pro Tyr  Arg Ser Tyr 
    2225                 2230                 2235             


Leu His  Asp Gly Glu Ser Gly  Asn Tyr Gly Thr Lys  Thr Asp Arg 
    2240                 2245                 2250             


Asp Arg  Cys Leu Thr Asp Ala  Leu Val Thr Val Arg  Pro Ser Leu 
    2255                 2260                 2265             


Leu Ile  Tyr Thr Leu Asp Phe  Lys Thr Ser Phe Leu  Ile Lys Asp 
    2270                 2275                 2280             


Leu Gly  Cys Cys Leu Gln Thr  Lys Lys Pro Pro Leu  Pro Ala Val 
    2285                 2290                 2295             


Val Cys  Leu Pro Asp Gln Glu  Leu Pro Thr Leu Phe  Pro Lys Val 
    2300                 2305                 2310             


Thr Gly  Phe Ser Arg Ala Gln  Ile Pro Asn Thr Val  Leu Leu Val 
    2315                 2320                 2325             


Pro Leu  Gly His His Phe Lys  Asn Ser Val Ala Pro  Pro Thr Tyr 
    2330                 2335                 2340             


Leu Ala  Leu Leu Ile Leu Leu  Pro Val Ala Ala Ala  Ser Gly Asp 
    2345                 2350                 2355             


Lys Ser  Cys Leu Thr Gly Leu  Asp Ser Arg Arg Leu  Pro Asp Lys 
    2360                 2365                 2370             


Ala Gln  Arg Ser Gly Thr Gly  Gly Ser Cys Thr Gln  Pro Ser Leu 
    2375                 2380                 2385             


Glu Arg  Thr Thr Tyr Thr Glu  Leu Arg Tyr Leu Gln  Arg Glu Leu 
    2390                 2395                 2400             


Glu Ser  Ala Thr Leu Pro Glu  Gly Arg Lys Ala Asp  Arg Tyr Pro 
    2405                 2410                 2415             


Val Ser  Gly Arg Val Gly Thr  Gly Glu Arg Thr Arg  Glu Leu Pro 
    2420                 2425                 2430             


Gly Gly  Asn Ala Trp Tyr Leu  Tyr Ser Pro Val Gly  Phe Arg His 
    2435                 2440                 2445             


Leu Leu  Glu Arg Arg Phe Leu  Cys Ser Ser Gly Gly  Arg Ser Leu 
    2450                 2455                 2460             


Trp Lys  Asn Ala Ser Asn Gly  Asp Ala Pro Arg Ala  Ala Ala Gly 
    2465                 2470                 2475             


Asp Gly  Gly Arg Asp Gly Tyr  Val Leu Pro Arg Val  Gly Leu Arg 
    2480                 2485                 2490             


Ile His  Ser Ser Pro Gln Glu  Leu Ile Gly Ser Asn  Ser Trp Ser 
    2495                 2500                 2505             


Gly Glu  Ser Val Ser Glu Val  Pro Pro Ala Ser Ile  Gln Val Glu 
    2510                 2515                 2520             


Val Ala  Arg Leu His Ala Pro  Arg Arg Asn Ala Gly  Arg Gln Thr 
    2525                 2530                 2535             


Arg Tyr  Arg Ala Ala Pro Thr  Ile His Ala Asn Pro  Phe His Val 
    2540                 2545                 2550             


Leu Ala  Glu Ala Ala Ile Ala  Val Thr Ile Ser Gly  Pro Met Ile 
    2555                 2560                 2565             


Glu Val  Arg Leu Val Arg Ala  Ala Ser Asp Pro Ser  Cys Pro Trp 
    2570                 2575                 2580             


Ser Ser  Ser Thr Cys Leu Asp  Ser Met Ala Cys Asn  Ala Gly Ile 
    2585                 2590                 2595             


Pro Met  Pro Pro Glu Ala Arg  Arg Ile Ile Met Gly  Lys Ala Ile 
    2600                 2605                 2610             


Gln Pro  Arg Val Gly Glu Leu  Phe Ala Lys Ala Ala  Ser Lys Lys 
    2615                 2620                 2625             


Ala Ser  Ser Leu Leu Leu Glu  Leu Arg Gly Arg Gly  Gly Leu Gly 
    2630                 2635                 2640             


Leu Cys  Ile Asn Lys Lys Asn  Ser Ala Met Ser 
    2645                 2650                 


<210>  3
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Influenza hemagglutinin cytoplasmic tail

<400>  3

Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
1               5                   10  


<210>  4
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Leucine-valine dipeptide cleavage site


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<400>  4

Tyr Xaa Xaa Leu 
1               


<210>  5
<211>  1249
<212>  PRT
<213>  Artificial

<220>
<223>  Modified SARS-CoV-2 spike protein

<400>  5

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Asn  Gly Ser Leu Gln Cys  Arg Ile Cys 
    1235                 1240                 1245             


Ile 
    


