                         SEQUENCE LISTING - RECTIFICATION (R91 PCT)

<110>  ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
 
<120>  METHOD OF PREPARATION OF cDNA LIBRARY USEFUL FOR EFFICIENT mRNA 
       SEQUENCING AND USES THEREOF

<130>  P2505EP00

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 1

<400>  1
ctcgagtagc ag                                                           12


<210>  2
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 2

<400>  2
cagcacacgt ca                                                           12


<210>  3
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 3

<400>  3
acagcgatcg ac                                                           12


<210>  4
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 4

<400>  4
ctctctacag ca                                                           12


<210>  5
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 5

<400>  5
tagtcgtcta gc                                                           12


<210>  6
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 6

<400>  6
catcagctgc ac                                                           12


<210>  7
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 7

<400>  7
tagtagcacg ca                                                           12


<210>  8
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 8

<400>  8
cagtcagctg ac                                                           12


<210>  9
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 9

<400>  9
cagcagtcta cg                                                           12


<210>  10
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 10

<400>  10
cagctagagc ac                                                           12


<210>  11
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 11

<400>  11
acagcagcgt ag                                                           12


<210>  12
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 12

<400>  12
actctacgcg ac                                                           12


<210>  13
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 13

<400>  13
ctgtcgagct ga                                                           12


<210>  14
<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  Barcode sequence 14

<400>  14
acagacgagt ca                                                           12


<210>  15
<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  Barcode sequence 15

<400>  15
ctatgatcta cg                                                           12


<210>  16
<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  Barcode sequence 16

<400>  16
ctcagagcag ac                                                           12


<210>  17
<211>  12
<212>  DNA
<213>  artificial sequence

<220>
<223>  Barcode sequence 17

<400>  17
acagagacta cg                                                           12


<210>  18
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 18

<400>  18
ctctgcacta gc                                                           12


<210>  19
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 19

<400>  19
actagtgacg ac                                                           12


<210>  20
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 20

<400>  20
tacgatgcgt ac                                                           12


<210>  21
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ACGAGACATCAC

<400>  21
acgagacatc ac                                                           12


<210>  22
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Barcode sequence 22

<400>  22
catcactgca ca                                                           12


<210>  23
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 1


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  23
ctacacgacg ctcttccgat ctctcgagta gcagnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  24
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 2


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  24
ctacacgacg ctcttccgat ctcagcacac gtcannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  25
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 3


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  25
ctacacgacg ctcttccgat ctacagcgat cgacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  26
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 4


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  26
ctacacgacg ctcttccgat ctctctctac agcannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  27
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 5


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  27
ctacacgacg ctcttccgat cttagtcgtc tagcnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  28
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 6


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  28
ctacacgacg ctcttccgat ctcatcagct gcacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  29
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 7


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  29
ctacacgacg ctcttccgat cttagtagca cgcannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  30
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 8


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  30
ctacacgacg ctcttccgat ctcagtcagc tgacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  31
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 9


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  31
ctacacgacg ctcttccgat ctcagcagtc tacgnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  32
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 10


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  32
ctacacgacg ctcttccgat ctcagctaga gcacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  33
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 11


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  33
ctacacgacg ctcttccgat ctacagcagc gtagnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  34
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 12


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  34
ctacacgacg ctcttccgat ctactctacg cgacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  35
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 13


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  35
ctacacgacg ctcttccgat ctctgtcgag ctgannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  36
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 14


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  36
ctacacgacg ctcttccgat ctacagacga gtcannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  37
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 15


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  37
ctacacgacg ctcttccgat ctctatgatc tacgnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  38
<211>  82
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligodT 16


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  38
ctacacgacg ctcttccgat ctctcagagc agacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  39
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 17


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein v stands for any nucleotide selected from A,C and G

<400>  39
ctacacgacg ctcttccgat ctacagagac tacgnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  40
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 18


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  40
ctacacgacg ctcttccgat ctctctgcac tagcnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  41
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 19


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  41
ctacacgacg ctcttccgat ctactagtga cgacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  42
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 20


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  42
ctacacgacg ctcttccgat cttacgatgc gtacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  43
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CTACACGACGCTCTTCCGATCTACGAGACATCACNNNNNNNNNNNVVVVVTTTTTTTTTTTTTTT
       TTTTTTTTTTTTTTTVN


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  43
ctacacgacg ctcttccgat ctacgagaca tcacnnnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  44
<211>  82
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 22


<220>
<221>  misc_feature
<222>  (35)..(45)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (46)..(50)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (81)..(81)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  44
ctacacgacg ctcttccgat ctcatcactg cacannnnnn nnnnnvvvvv tttttttttt       60

tttttttttt tttttttttt vn                                                82


<210>  45
<211>  85
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligodT 23


<220>
<221>  misc_feature
<222>  (26)..(48)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<220>
<221>  misc_feature
<222>  (49)..(53)
<223>  wherein v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  wherein  v stands for any nucleotide selected from A,C and G

<220>
<221>  misc_feature
<222>  (85)..(85)
<223>  wherein n stands for any nucleotide selected from A,C,G and T

<400>  45
aagcagtggt atcaacgcag agtacnnnnn nnnnnnnnnn nnnnnnnnvv vvvttttttt       60

tttttttttt tttttttttt tttvn                                             85


