<110>    INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
         MediSpan Co., Ltd

<120>    Composition for preventing or treating non-alcoholic fatty liver
         disease or metabolic syndrome comprising flagellin fusion protein

<130>    OP20-0098/PCT

<150>    KR 10-2020-0136280
<151>    2020-10-20

<160>    28

<170>    KoPatentIn 3.0

<210>    1
<211>    278
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Bacillus subtilis(Bs) flagellin


<400>    1
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Pro Gly Lys
        275            


<210>    2
<211>    505
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Salmonella dublin(Sd) flagellin


<400>    2
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn
  1               5                  10                  15 

Asn Leu Asn Lys Ser Gln Ser Ser Leu Ser Ser Ala Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ser
                 85                  90                  95 

Val Gln Ala Thr Asn Gly Thr Asn Ser Asp Ser Asp Leu Lys Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Gln Gln Arg Leu Glu Glu Ile Asp Arg Val Ser Asn
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ser Gln Asp Asn Gln Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Gln Lys Ile Asp Val Lys Ser Leu Gly Leu Asp Gly Phe Asn Val Asn
                165                 170                 175 

Gly Pro Lys Glu Ala Thr Val Gly Asp Leu Lys Ser Ser Phe Lys Asn
            180                 185                 190 

Val Thr Gly Tyr Asp Thr Tyr Ala Ala Gly Ala Asp Lys Tyr Arg Val
        195                 200                 205 

Asp Ile Asn Ser Gly Ala Val Val Thr Asp Ala Val Ala Pro Asp Lys
    210                 215                 220 

Val Tyr Val Asn Ala Ala Asn Gly Gln Leu Thr Thr Asp Asp Ala Glu
225                 230                 235                 240 

Asn Asn Thr Ala Val Asp Leu Phe Lys Thr Thr Lys Ser Thr Ala Gly
                245                 250                 255 

Thr Ala Glu Ala Lys Ala Ile Ala Gly Ala Ile Lys Gly Gly Lys Glu
            260                 265                 270 

Gly Asp Thr Phe Asp Tyr Lys Gly Val Thr Phe Thr Ile Asp Thr Lys
        275                 280                 285 

Thr Gly Asp Asp Gly Asn Gly Lys Val Ser Thr Thr Ile Asn Gly Glu
    290                 295                 300 

Lys Val Thr Leu Thr Val Ala Asp Ile Ala Ile Gly Ala Ala Asp Val
305                 310                 315                 320 

Asn Ala Ala Thr Leu Gln Ser Ser Lys Asn Val Tyr Thr Ser Val Val
                325                 330                 335 

Asn Gly Gln Phe Thr Phe Asp Asp Lys Thr Lys Asn Glu Ser Ala Lys
            340                 345                 350 

Leu Ser Asp Leu Glu Ala Asn Asn Ala Val Lys Gly Glu Ser Lys Ile
        355                 360                 365 

Thr Val Asn Gly Ala Glu Tyr Thr Ala Asn Ala Thr Gly Asp Lys Ile
    370                 375                 380 

Thr Leu Ala Gly Lys Thr Met Phe Ile Asp Lys Thr Ala Ser Gly Val
385                 390                 395                 400 

Ser Thr Leu Ile Asn Glu Asp Ala Ala Ala Ala Lys Lys Ser Thr Ala
                405                 410                 415 

Asn Pro Leu Ala Ser Ile Asp Ser Ala Leu Ser Lys Val Asp Ala Val
            420                 425                 430 

Arg Ser Ser Leu Gly Ala Ile Gln Asn Arg Phe Asp Ser Ala Ile Thr
        435                 440                 445 

Asn Leu Gly Asn Thr Val Thr Asn Leu Asn Ser Ala Arg Ser Arg Ile
    450                 455                 460 

Glu Asp Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln
465                 470                 475                 480 

Ile Leu Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala Asn Gln Val
                485                 490                 495 

Pro Gln Asn Val Leu Ser Leu Leu Arg
            500                 505


<210>    3
<211>    387
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Pseudomonas aeruginosa (Pa) flagellin


<400>    3
Met Ala Leu Thr Val Asn Thr Asn Ile Ala Ser Leu Asn Thr Gln Arg
  1               5                  10                  15 

Asn Gln Asn Asn Ser Ser Ala Ser Leu Asn Thr Ser Leu Gln Arg Leu
             20                  25                  30 

Ser Thr Gly Ser Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Leu
         35                  40                  45 

Gln Ile Ala Asn Arg Leu Thr Ser Gln Val Asn Gly Leu Asn Val Ala
     50                  55                  60 

Thr Lys Asn Ala Asn Asp Gly Ile Ser Leu Ala Gln Thr Ala Glu Gly
 65                  70                  75                  80 

Ala Leu Gln Gln Ser Thr Asn Ile Leu Gln Arg Met Arg Asp Leu Ser
                 85                  90                  95 

Leu Gln Ser Ala Asn Gly Ser Asn Ser Asp Ser Glu Arg Thr Ala Leu
            100                 105                 110 

Asn Gly Glu Val Lys Gln Leu Gln Lys Glu Leu Asp Arg Ile Ser Asn
        115                 120                 125 

Thr Thr Thr Phe Gly Gly Arg Lys Leu Leu Asp Gly Ser Phe Gly Val
    130                 135                 140 

Ala Ser Phe Gln Val Gly Ser Ala Ala Asn Glu Ile Ile Ser Val Gly
145                 150                 155                 160 

Ile Asp Glu Met Ser Ala Glu Ser Leu Asn Gly Thr Tyr Phe Lys Ala
                165                 170                 175 

Asp Gly Gly Gly Ala Val Thr Ala Ala Thr Ala Ser Gly Thr Val Asp
            180                 185                 190 

Ile Ala Ile Asp Ile Thr Gly Gly Ser Ala Val Asn Val Lys Val Asp
        195                 200                 205 

Met Lys Gly Asn Glu Thr Ala Glu Gln Ala Ala Ala Lys Ile Ala Ala
    210                 215                 220 

Ala Val Asn Asp Ala Asn Val Gly Ile Gly Ala Phe Thr Asp Gly Ala
225                 230                 235                 240 

Gln Ile Ser Tyr Val Ser Lys Ala Ser Ala Asp Gly Thr Thr Ser Ala
                245                 250                 255 

Val Ser Gly Val Ala Ile Thr Asp Thr Gly Ser Thr Gly Ala Gly Thr
            260                 265                 270 

Ala Ala Gly Thr Thr Thr Phe Thr Glu Ala Asn Asp Thr Val Ala Lys
        275                 280                 285 

Ile Asp Ile Ser Thr Ala Lys Gly Ala Gln Ser Ala Val Leu Val Ile
    290                 295                 300 

Asp Glu Ala Ile Lys Gln Ile Asp Ala Gln Arg Ala Asp Leu Gly Ala
305                 310                 315                 320 

Val Gln Asn Arg Phe Asp Asn Thr Ile Asn Asn Leu Lys Asn Ile Gly
                325                 330                 335 

Glu Asn Val Ser Ala Ala Arg Gly Arg Ile Glu Asp Thr Asp Phe Ala
            340                 345                 350 

Ala Glu Thr Ala Asn Leu Thr Lys Asn Gln Val Leu Gln Gln Ala Gly
        355                 360                 365 

Thr Ala Ile Leu Ala Gln Ala Asn Gln Leu Pro Gln Ser Val Leu Ser
    370                 375                 380 

Leu Leu Arg
385        


<210>    4
<211>    550
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Shigella flexneri (Sf) flagellin


<400>    4
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Ile Thr Gln Asn
  1               5                  10                  15 

Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser Ser Ser Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ala Arg Asn Ala Asn Asp Gly Ile Ser Val Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln Arg Ile Arg Glu Leu Thr
                 85                  90                  95 

Val Gln Ala Ser Thr Gly Thr Asn Ser Asp Ser Asp Leu Asp Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu Ile Asp Arg Val Ser Gly
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Asn Val Leu Ala Lys Asp Gly Ser Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Gly Gln Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Lys Lys Ile Asp Ser Asp Thr Leu Gly Leu Asn Gly Phe Asn Val Asn
                165                 170                 175 

Gly Gly Gly Ala Val Ala Asn Thr Ala Ala Ser Lys Ala Asp Leu Val
            180                 185                 190 

Ala Ala Asn Ala Thr Val Val Gly Asn Lys Tyr Thr Val Ser Ala Gly
        195                 200                 205 

Tyr Asp Ala Ala Lys Ala Ser Asp Leu Leu Ala Gly Val Ser Asp Gly
    210                 215                 220 

Asp Thr Val Gln Ala Thr Ile Asn Asn Gly Phe Gly Thr Ala Ala Ser
225                 230                 235                 240 

Ala Thr Asn Tyr Lys Tyr Asp Ser Ala Ser Lys Ser Tyr Ser Phe Asp
                245                 250                 255 

Thr Thr Thr Ala Ser Ala Ala Asp Val Gln Lys Tyr Leu Thr Pro Gly
            260                 265                 270 

Val Gly Asp Thr Ala Lys Gly Thr Ile Thr Ile Asp Gly Ser Ala Gln
        275                 280                 285 

Asp Val Gln Ile Ser Ser Asp Gly Lys Ile Thr Ala Ser Asn Gly Asp
    290                 295                 300 

Lys Leu Tyr Ile Asp Thr Thr Gly Arg Leu Thr Lys Asn Gly Ser Gly
305                 310                 315                 320 

Ala Ser Leu Thr Glu Ala Ser Leu Ser Thr Leu Ala Ala Asn Asn Thr
                325                 330                 335 

Lys Ala Thr Thr Ile Asp Ile Gly Gly Thr Ser Ile Ser Phe Thr Gly
            340                 345                 350 

Asn Ser Thr Thr Pro Asp Thr Ile Thr Tyr Ser Val Thr Gly Ala Lys
        355                 360                 365 

Val Asp Gln Ala Ala Phe Asp Lys Ala Val Ser Thr Ser Gly Asn Asn
    370                 375                 380 

Val Asp Phe Thr Thr Ala Gly Tyr Ser Val Asn Gly Thr Thr Gly Ala
385                 390                 395                 400 

Val Thr Lys Gly Val Asp Ser Val Tyr Val Asp Asn Asn Glu Ala Leu
                405                 410                 415 

Thr Thr Ser Asp Thr Val Asp Phe Tyr Leu Gln Asp Asp Gly Ser Val
            420                 425                 430 

Thr Asn Gly Ser Gly Lys Ala Val Tyr Lys Asp Ala Asp Gly Lys Leu
        435                 440                 445 

Thr Thr Asp Ala Glu Thr Lys Ala Ala Thr Thr Ala Asp Pro Leu Lys
    450                 455                 460 

Ala Leu Asp Glu Ala Ile Ser Ser Ile Asp Lys Phe Arg Ser Ser Leu
465                 470                 475                 480 

Gly Ala Val Gln Asn Arg Leu Asp Ser Ala Val Thr Asn Leu Asn Asn
                485                 490                 495 

Thr Thr Thr Asn Leu Ser Glu Ala Gln Ser Arg Ile Gln Asp Ala Asp
            500                 505                 510 

Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln Ile Ile Gln Gln
        515                 520                 525 

Ala Gly Asn Ser Val Leu Ala Lys Ala Asn Gln Val Pro Gln Gln Val
    530                 535                 540 

Leu Ser Leu Leu Gln Gly
545                 550


<210>    5
<211>    498
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Escherichia coli (Ec) flagellin


<400>    5
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Ile Thr Gln Asn
  1               5                  10                  15 

Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser Ser Ser Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ala Arg Asn Ala Asn Asp Gly Ile Ser Val Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Thr
                 85                  90                  95 

Val Gln Ala Thr Thr Gly Thr Asn Ser Glu Ser Asp Leu Ser Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu Ile Asp Arg Val Ser Gly
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Asn Val Leu Ala Lys Asn Gly Ser Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Asn Gln Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Lys Gln Ile Asp Ala Lys Thr Leu Gly Leu Asp Gly Phe Ser Val Lys
                165                 170                 175 

Asn Asn Asp Thr Val Thr Thr Ser Ala Pro Val Thr Ala Phe Gly Ala
            180                 185                 190 

Thr Thr Thr Asn Asn Ile Lys Leu Thr Gly Ile Thr Leu Ser Thr Glu
        195                 200                 205 

Ala Ala Thr Asp Thr Gly Gly Thr Asn Pro Ala Ser Ile Glu Gly Val
    210                 215                 220 

Tyr Thr Asp Asn Gly Asn Asp Tyr Tyr Ala Lys Ile Thr Gly Gly Asp
225                 230                 235                 240 

Asn Asp Gly Lys Tyr Tyr Ala Val Thr Val Ala Asn Asp Gly Thr Val
                245                 250                 255 

Thr Met Ala Thr Gly Ala Thr Ala Asn Ala Thr Val Thr Asp Ala Asn
            260                 265                 270 

Thr Thr Lys Ala Thr Thr Ile Thr Ser Gly Gly Thr Pro Val Gln Ile
        275                 280                 285 

Asp Asn Thr Ala Gly Ser Ala Thr Ala Asn Leu Gly Ala Val Ser Leu
    290                 295                 300 

Val Lys Leu Gln Asp Ser Lys Gly Asn Asp Thr Asp Thr Tyr Ala Leu
305                 310                 315                 320 

Lys Asp Thr Asn Gly Asn Leu Tyr Ala Ala Asp Val Asn Glu Thr Thr
                325                 330                 335 

Gly Ala Val Ser Val Lys Thr Ile Thr Tyr Thr Asp Ser Ser Gly Ala
            340                 345                 350 

Ala Ser Ser Pro Thr Ala Val Lys Leu Gly Gly Asp Asp Gly Lys Thr
        355                 360                 365 

Glu Val Val Asp Ile Asp Gly Lys Thr Tyr Asp Ser Ala Asp Leu Asn
    370                 375                 380 

Gly Gly Asn Leu Gln Thr Gly Leu Thr Ala Gly Gly Glu Ala Leu Thr
385                 390                 395                 400 

Ala Val Ala Asn Gly Lys Thr Thr Asp Pro Leu Lys Ala Leu Asp Asp
                405                 410                 415 

Ala Ile Ala Ser Val Asp Lys Phe Arg Ser Ser Leu Gly Ala Val Gln
            420                 425                 430 

Asn Arg Leu Asp Ser Ala Val Thr Asn Leu Asn Asn Thr Thr Thr Asn
        435                 440                 445 

Leu Ser Glu Ala Gln Ser Arg Ile Gln Asp Ala Asp Tyr Ala Thr Glu
    450                 455                 460 

Val Ser Asn Met Ser Lys Ala Gln Ile Ile Gln Gln Ala Gly Asn Ser
465                 470                 475                 480 

Val Leu Ala Lys Ala Asn Gln Val Pro Gln Gln Val Leu Ser Leu Leu
                485                 490                 495 

Gln Gly
       


<210>    6
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Human IgG4 Fc region


<400>    6
Ala Met Val Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Leu Gly Lys
225                


<210>    7
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Mouse IgG1 Fc region


<400>    7
Thr Met Val Arg Ser Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu
  1               5                  10                  15 

Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr
             20                  25                  30 

Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser Lys
         35                  40                  45 

Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val
     50                  55                  60 

His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe
 65                  70                  75                  80 

Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly
                 85                  90                  95 

Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile
            100                 105                 110 

Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val
        115                 120                 125 

Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser
    130                 135                 140 

Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu
145                 150                 155                 160 

Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro
                165                 170                 175 

Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val
            180                 185                 190 

Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu
        195                 200                 205 

His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser His Ser
    210                 215                 220 

Pro Gly Lys
225        


<210>    8
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Linker 1


<400>    8
Gly Gly Gly Gly Ser
  1               5


<210>    9
<211>    15
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Linker 2


<400>    9
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
  1               5                  10                  15


<210>    10
<211>    509
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4-Fc-Bsflagellin fusion protein


<400>    10
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Pro Pro Cys
        275                 280                 285 

Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu
    290                 295                 300 

Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
305                 310                 315                 320 

Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln
                325                 330                 335 

Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
            340                 345                 350 

Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
        355                 360                 365 

Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
    370                 375                 380 

Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys
385                 390                 395                 400 

Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
                405                 410                 415 

Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
            420                 425                 430 

Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
        435                 440                 445 

Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
    450                 455                 460 

Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln
465                 470                 475                 480 

Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
                485                 490                 495 

His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
            500                 505                


<210>    11
<211>    507
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    mIgG1-Fc-Bsflagellin fusion protein (with linker 1)


<400>    11
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Thr Met Val Arg Ser Gly Cys Lys
        275                 280                 285 

Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro
    290                 295                 300 

Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr
305                 310                 315                 320 

Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser
                325                 330                 335 

Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg
            340                 345                 350 

Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile
        355                 360                 365 

Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn
    370                 375                 380 

Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys
385                 390                 395                 400 

Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu
                405                 410                 415 

Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe
            420                 425                 430 

Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala
        435                 440                 445 

Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr
    450                 455                 460 

Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly
465                 470                 475                 480 

Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His
                485                 490                 495 

Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys
            500                 505        


<210>    12
<211>    517
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    mIgG1-Fc-Bsflagellin fusion protein (with linker 2)


<400>    12
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
        275                 280                 285 

Gly Ser Thr Met Val Arg Ser Gly Cys Lys Pro Cys Ile Cys Thr Val
    290                 295                 300 

Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val
305                 310                 315                 320 

Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile
                325                 330                 335 

Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val
            340                 345                 350 

Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser
        355                 360                 365 

Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu
    370                 375                 380 

Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala
385                 390                 395                 400 

Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro
                405                 410                 415 

Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys
            420                 425                 430 

Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr
        435                 440                 445 

Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr
    450                 455                 460 

Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu
465                 470                 475                 480 

Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser
                485                 490                 495 

Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser
            500                 505                 510 

His Ser Pro Gly Lys
        515        


<210>    13
<211>    761
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4-Fc-Sdflagellin  fusion protein (with linker 1)


<400>    13
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu
  1               5                  10                  15 

Val Thr Asn Ser Ile Ser Met Ala Gln Val Ile Asn Thr Asn Ser Leu
             20                  25                  30 

Ser Leu Leu Thr Gln Asn Asn Leu Asn Lys Ser Gln Ser Ser Leu Ser
         35                  40                  45 

Ser Ala Ile Glu Arg Leu Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys
     50                  55                  60 

Asp Asp Ala Ala Gly Gln Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile
 65                  70                  75                  80 

Lys Gly Leu Thr Gln Ala Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile
                 85                  90                  95 

Ala Gln Thr Thr Glu Gly Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln
            100                 105                 110 

Arg Val Arg Glu Leu Ser Val Gln Ala Thr Asn Gly Thr Asn Ser Asp
        115                 120                 125 

Ser Asp Leu Lys Ser Ile Gln Asp Glu Ile Gln Gln Arg Leu Glu Glu
    130                 135                 140 

Ile Asp Arg Val Ser Asn Gln Thr Gln Phe Asn Gly Val Lys Val Leu
145                 150                 155                 160 

Ser Gln Asp Asn Gln Met Lys Ile Gln Val Gly Ala Asn Asp Gly Glu
                165                 170                 175 

Thr Ile Thr Ile Asp Leu Gln Lys Ile Asp Val Lys Ser Leu Gly Leu
            180                 185                 190 

Asp Gly Phe Asn Val Asn Gly Pro Lys Glu Ala Thr Val Gly Asp Leu
        195                 200                 205 

Lys Ser Ser Phe Lys Asn Val Thr Gly Tyr Asp Thr Tyr Ala Ala Gly
    210                 215                 220 

Ala Asp Lys Tyr Arg Val Asp Ile Asn Ser Gly Ala Val Val Thr Asp
225                 230                 235                 240 

Ala Val Ala Pro Asp Lys Val Tyr Val Asn Ala Ala Asn Gly Gln Leu
                245                 250                 255 

Thr Thr Asp Asp Ala Glu Asn Asn Thr Ala Val Asp Leu Phe Lys Thr
            260                 265                 270 

Thr Lys Ser Thr Ala Gly Thr Ala Glu Ala Lys Ala Ile Ala Gly Ala
        275                 280                 285 

Ile Lys Gly Gly Lys Glu Gly Asp Thr Phe Asp Tyr Lys Gly Val Thr
    290                 295                 300 

Phe Thr Ile Asp Thr Lys Thr Gly Asp Asp Gly Asn Gly Lys Val Ser
305                 310                 315                 320 

Thr Thr Ile Asn Gly Glu Lys Val Thr Leu Thr Val Ala Asp Ile Ala
                325                 330                 335 

Ile Gly Ala Ala Asp Val Asn Ala Ala Thr Leu Gln Ser Ser Lys Asn
            340                 345                 350 

Val Tyr Thr Ser Val Val Asn Gly Gln Phe Thr Phe Asp Asp Lys Thr
        355                 360                 365 

Lys Asn Glu Ser Ala Lys Leu Ser Asp Leu Glu Ala Asn Asn Ala Val
    370                 375                 380 

Lys Gly Glu Ser Lys Ile Thr Val Asn Gly Ala Glu Tyr Thr Ala Asn
385                 390                 395                 400 

Ala Thr Gly Asp Lys Ile Thr Leu Ala Gly Lys Thr Met Phe Ile Asp
                405                 410                 415 

Lys Thr Ala Ser Gly Val Ser Thr Leu Ile Asn Glu Asp Ala Ala Ala
            420                 425                 430 

Ala Lys Lys Ser Thr Ala Asn Pro Leu Ala Ser Ile Asp Ser Ala Leu
        435                 440                 445 

Ser Lys Val Asp Ala Val Arg Ser Ser Leu Gly Ala Ile Gln Asn Arg
    450                 455                 460 

Phe Asp Ser Ala Ile Thr Asn Leu Gly Asn Thr Val Thr Asn Leu Asn
465                 470                 475                 480 

Ser Ala Arg Ser Arg Ile Glu Asp Ala Asp Tyr Ala Thr Glu Val Ser
                485                 490                 495 

Asn Met Ser Lys Ala Gln Ile Leu Gln Gln Ala Gly Thr Ser Val Leu
            500                 505                 510 

Ala Gln Ala Asn Gln Val Pro Gln Asn Val Leu Ser Leu Leu Arg Gly
        515                 520                 525 

Gly Gly Gly Ser Ala Met Val Arg Ser Pro Pro Cys Pro Ser Cys Pro
    530                 535                 540 

Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
545                 550                 555                 560 

Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
                565                 570                 575 

Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr
            580                 585                 590 

Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
        595                 600                 605 

Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
    610                 615                 620 

Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
625                 630                 635                 640 

Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
                645                 650                 655 

Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met
            660                 665                 670 

Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
        675                 680                 685 

Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
    690                 695                 700 

Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
705                 710                 715                 720 

Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val
                725                 730                 735 

Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
            740                 745                 750 

Lys Ser Leu Ser Leu Ser Leu Gly Lys
        755                 760    


<210>    14
<211>    643
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4-Fc-Paflagellin  fusion protein (with linker 1)


<400>    14
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu
  1               5                  10                  15 

Val Thr Asn Ser Ile Ser Met Ala Leu Thr Val Asn Thr Asn Ile Ala
             20                  25                  30 

Ser Leu Asn Thr Gln Arg Asn Gln Asn Asn Ser Ser Ala Ser Leu Asn
         35                  40                  45 

Thr Ser Leu Gln Arg Leu Ser Thr Gly Ser Arg Ile Asn Ser Ala Lys
     50                  55                  60 

Asp Asp Ala Ala Gly Leu Gln Ile Ala Asn Arg Leu Thr Ser Gln Val
 65                  70                  75                  80 

Asn Gly Leu Asn Val Ala Thr Lys Asn Ala Asn Asp Gly Ile Ser Leu
                 85                  90                  95 

Ala Gln Thr Ala Glu Gly Ala Leu Gln Gln Ser Thr Asn Ile Leu Gln
            100                 105                 110 

Arg Met Arg Asp Leu Ser Leu Gln Ser Ala Asn Gly Ser Asn Ser Asp
        115                 120                 125 

Ser Glu Arg Thr Ala Leu Asn Gly Glu Val Lys Gln Leu Gln Lys Glu
    130                 135                 140 

Leu Asp Arg Ile Ser Asn Thr Thr Thr Phe Gly Gly Arg Lys Leu Leu
145                 150                 155                 160 

Asp Gly Ser Phe Gly Val Ala Ser Phe Gln Val Gly Ser Ala Ala Asn
                165                 170                 175 

Glu Ile Ile Ser Val Gly Ile Asp Glu Met Ser Ala Glu Ser Leu Asn
            180                 185                 190 

Gly Thr Tyr Phe Lys Ala Asp Gly Gly Gly Ala Val Thr Ala Ala Thr
        195                 200                 205 

Ala Ser Gly Thr Val Asp Ile Ala Ile Asp Ile Thr Gly Gly Ser Ala
    210                 215                 220 

Val Asn Val Lys Val Asp Met Lys Gly Asn Glu Thr Ala Glu Gln Ala
225                 230                 235                 240 

Ala Ala Lys Ile Ala Ala Ala Val Asn Asp Ala Asn Val Gly Ile Gly
                245                 250                 255 

Ala Phe Thr Asp Gly Ala Gln Ile Ser Tyr Val Ser Lys Ala Ser Ala
            260                 265                 270 

Asp Gly Thr Thr Ser Ala Val Ser Gly Val Ala Ile Thr Asp Thr Gly
        275                 280                 285 

Ser Thr Gly Ala Gly Thr Ala Ala Gly Thr Thr Thr Phe Thr Glu Ala
    290                 295                 300 

Asn Asp Thr Val Ala Lys Ile Asp Ile Ser Thr Ala Lys Gly Ala Gln
305                 310                 315                 320 

Ser Ala Val Leu Val Ile Asp Glu Ala Ile Lys Gln Ile Asp Ala Gln
                325                 330                 335 

Arg Ala Asp Leu Gly Ala Val Gln Asn Arg Phe Asp Asn Thr Ile Asn
            340                 345                 350 

Asn Leu Lys Asn Ile Gly Glu Asn Val Ser Ala Ala Arg Gly Arg Ile
        355                 360                 365 

Glu Asp Thr Asp Phe Ala Ala Glu Thr Ala Asn Leu Thr Lys Asn Gln
    370                 375                 380 

Val Leu Gln Gln Ala Gly Thr Ala Ile Leu Ala Gln Ala Asn Gln Leu
385                 390                 395                 400 

Pro Gln Ser Val Leu Ser Leu Leu Arg Gly Gly Gly Gly Ser Ala Met
                405                 410                 415 

Val Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly
            420                 425                 430 

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
        435                 440                 445 

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln
    450                 455                 460 

Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val
465                 470                 475                 480 

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr
                485                 490                 495 

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly
            500                 505                 510 

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile
        515                 520                 525 

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val
    530                 535                 540 

Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser
545                 550                 555                 560 

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
                565                 570                 575 

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
            580                 585                 590 

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val
        595                 600                 605 

Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met
    610                 615                 620 

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
625                 630                 635                 640 

Leu Gly Lys
           


<210>    15
<211>    806
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4-Fc-Sfflagellin fusion protein (with linker 1)


<400>    15
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu
  1               5                  10                  15 

Val Thr Asn Ser Ile Ser Met Ala Gln Val Ile Asn Thr Asn Ser Leu
             20                  25                  30 

Ser Leu Ile Thr Gln Asn Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser
         35                  40                  45 

Ser Ser Ile Glu Arg Leu Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys
     50                  55                  60 

Asp Asp Ala Ala Gly Gln Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile
 65                  70                  75                  80 

Lys Gly Leu Thr Gln Ala Ala Arg Asn Ala Asn Asp Gly Ile Ser Val
                 85                  90                  95 

Ala Gln Thr Thr Glu Gly Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln
            100                 105                 110 

Arg Ile Arg Glu Leu Thr Val Gln Ala Ser Thr Gly Thr Asn Ser Asp
        115                 120                 125 

Ser Asp Leu Asp Ser Ile Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu
    130                 135                 140 

Ile Asp Arg Val Ser Gly Gln Thr Gln Phe Asn Gly Val Asn Val Leu
145                 150                 155                 160 

Ala Lys Asp Gly Ser Met Lys Ile Gln Val Gly Ala Asn Asp Gly Gln
                165                 170                 175 

Thr Ile Thr Ile Asp Leu Lys Lys Ile Asp Ser Asp Thr Leu Gly Leu
            180                 185                 190 

Asn Gly Phe Asn Val Asn Gly Gly Gly Ala Val Ala Asn Thr Ala Ala
        195                 200                 205 

Ser Lys Ala Asp Leu Val Ala Ala Asn Ala Thr Val Val Gly Asn Lys
    210                 215                 220 

Tyr Thr Val Ser Ala Gly Tyr Asp Ala Ala Lys Ala Ser Asp Leu Leu
225                 230                 235                 240 

Ala Gly Val Ser Asp Gly Asp Thr Val Gln Ala Thr Ile Asn Asn Gly
                245                 250                 255 

Phe Gly Thr Ala Ala Ser Ala Thr Asn Tyr Lys Tyr Asp Ser Ala Ser
            260                 265                 270 

Lys Ser Tyr Ser Phe Asp Thr Thr Thr Ala Ser Ala Ala Asp Val Gln
        275                 280                 285 

Lys Tyr Leu Thr Pro Gly Val Gly Asp Thr Ala Lys Gly Thr Ile Thr
    290                 295                 300 

Ile Asp Gly Ser Ala Gln Asp Val Gln Ile Ser Ser Asp Gly Lys Ile
305                 310                 315                 320 

Thr Ala Ser Asn Gly Asp Lys Leu Tyr Ile Asp Thr Thr Gly Arg Leu
                325                 330                 335 

Thr Lys Asn Gly Ser Gly Ala Ser Leu Thr Glu Ala Ser Leu Ser Thr
            340                 345                 350 

Leu Ala Ala Asn Asn Thr Lys Ala Thr Thr Ile Asp Ile Gly Gly Thr
        355                 360                 365 

Ser Ile Ser Phe Thr Gly Asn Ser Thr Thr Pro Asp Thr Ile Thr Tyr
    370                 375                 380 

Ser Val Thr Gly Ala Lys Val Asp Gln Ala Ala Phe Asp Lys Ala Val
385                 390                 395                 400 

Ser Thr Ser Gly Asn Asn Val Asp Phe Thr Thr Ala Gly Tyr Ser Val
                405                 410                 415 

Asn Gly Thr Thr Gly Ala Val Thr Lys Gly Val Asp Ser Val Tyr Val
            420                 425                 430 

Asp Asn Asn Glu Ala Leu Thr Thr Ser Asp Thr Val Asp Phe Tyr Leu
        435                 440                 445 

Gln Asp Asp Gly Ser Val Thr Asn Gly Ser Gly Lys Ala Val Tyr Lys
    450                 455                 460 

Asp Ala Asp Gly Lys Leu Thr Thr Asp Ala Glu Thr Lys Ala Ala Thr
465                 470                 475                 480 

Thr Ala Asp Pro Leu Lys Ala Leu Asp Glu Ala Ile Ser Ser Ile Asp
                485                 490                 495 

Lys Phe Arg Ser Ser Leu Gly Ala Val Gln Asn Arg Leu Asp Ser Ala
            500                 505                 510 

Val Thr Asn Leu Asn Asn Thr Thr Thr Asn Leu Ser Glu Ala Gln Ser
        515                 520                 525 

Arg Ile Gln Asp Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys
    530                 535                 540 

Ala Gln Ile Ile Gln Gln Ala Gly Asn Ser Val Leu Ala Lys Ala Asn
545                 550                 555                 560 

Gln Val Pro Gln Gln Val Leu Ser Leu Leu Gln Gly Gly Gly Gly Gly
                565                 570                 575 

Ser Ala Met Val Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu
            580                 585                 590 

Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
        595                 600                 605 

Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
    610                 615                 620 

Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly
625                 630                 635                 640 

Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn
                645                 650                 655 

Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
            660                 665                 670 

Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro
        675                 680                 685 

Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
    690                 695                 700 

Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn
705                 710                 715                 720 

Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
                725                 730                 735 

Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
            740                 745                 750 

Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg
        755                 760                 765 

Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys
    770                 775                 780 

Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
785                 790                 795                 800 

Ser Leu Ser Leu Gly Lys
                805    


<210>    16
<211>    754
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4-Fc-Ecflagellin fusion protein (with linker 1)


<400>    16
Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu
  1               5                  10                  15 

Val Thr Asn Ser Ile Ser Met Ala Gln Val Ile Asn Thr Asn Ser Leu
             20                  25                  30 

Ser Leu Ile Thr Gln Asn Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser
         35                  40                  45 

Ser Ser Ile Glu Arg Leu Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys
     50                  55                  60 

Asp Asp Ala Ala Gly Gln Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile
 65                  70                  75                  80 

Lys Gly Leu Thr Gln Ala Ala Arg Asn Ala Asn Asp Gly Ile Ser Val
                 85                  90                  95 

Ala Gln Thr Thr Glu Gly Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln
            100                 105                 110 

Arg Val Arg Glu Leu Thr Val Gln Ala Thr Thr Gly Thr Asn Ser Glu
        115                 120                 125 

Ser Asp Leu Ser Ser Ile Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu
    130                 135                 140 

Ile Asp Arg Val Ser Gly Gln Thr Gln Phe Asn Gly Val Asn Val Leu
145                 150                 155                 160 

Ala Lys Asn Gly Ser Met Lys Ile Gln Val Gly Ala Asn Asp Asn Gln
                165                 170                 175 

Thr Ile Thr Ile Asp Leu Lys Gln Ile Asp Ala Lys Thr Leu Gly Leu
            180                 185                 190 

Asp Gly Phe Ser Val Lys Asn Asn Asp Thr Val Thr Thr Ser Ala Pro
        195                 200                 205 

Val Thr Ala Phe Gly Ala Thr Thr Thr Asn Asn Ile Lys Leu Thr Gly
    210                 215                 220 

Ile Thr Leu Ser Thr Glu Ala Ala Thr Asp Thr Gly Gly Thr Asn Pro
225                 230                 235                 240 

Ala Ser Ile Glu Gly Val Tyr Thr Asp Asn Gly Asn Asp Tyr Tyr Ala
                245                 250                 255 

Lys Ile Thr Gly Gly Asp Asn Asp Gly Lys Tyr Tyr Ala Val Thr Val
            260                 265                 270 

Ala Asn Asp Gly Thr Val Thr Met Ala Thr Gly Ala Thr Ala Asn Ala
        275                 280                 285 

Thr Val Thr Asp Ala Asn Thr Thr Lys Ala Thr Thr Ile Thr Ser Gly
    290                 295                 300 

Gly Thr Pro Val Gln Ile Asp Asn Thr Ala Gly Ser Ala Thr Ala Asn
305                 310                 315                 320 

Leu Gly Ala Val Ser Leu Val Lys Leu Gln Asp Ser Lys Gly Asn Asp
                325                 330                 335 

Thr Asp Thr Tyr Ala Leu Lys Asp Thr Asn Gly Asn Leu Tyr Ala Ala
            340                 345                 350 

Asp Val Asn Glu Thr Thr Gly Ala Val Ser Val Lys Thr Ile Thr Tyr
        355                 360                 365 

Thr Asp Ser Ser Gly Ala Ala Ser Ser Pro Thr Ala Val Lys Leu Gly
    370                 375                 380 

Gly Asp Asp Gly Lys Thr Glu Val Val Asp Ile Asp Gly Lys Thr Tyr
385                 390                 395                 400 

Asp Ser Ala Asp Leu Asn Gly Gly Asn Leu Gln Thr Gly Leu Thr Ala
                405                 410                 415 

Gly Gly Glu Ala Leu Thr Ala Val Ala Asn Gly Lys Thr Thr Asp Pro
            420                 425                 430 

Leu Lys Ala Leu Asp Asp Ala Ile Ala Ser Val Asp Lys Phe Arg Ser
        435                 440                 445 

Ser Leu Gly Ala Val Gln Asn Arg Leu Asp Ser Ala Val Thr Asn Leu
    450                 455                 460 

Asn Asn Thr Thr Thr Asn Leu Ser Glu Ala Gln Ser Arg Ile Gln Asp
465                 470                 475                 480 

Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln Ile Ile
                485                 490                 495 

Gln Gln Ala Gly Asn Ser Val Leu Ala Lys Ala Asn Gln Val Pro Gln
            500                 505                 510 

Gln Val Leu Ser Leu Leu Gln Gly Gly Gly Gly Gly Ser Ala Met Val
        515                 520                 525 

Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly
    530                 535                 540 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
545                 550                 555                 560 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                565                 570                 575 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            580                 585                 590 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        595                 600                 605 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    610                 615                 620 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
625                 630                 635                 640 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                645                 650                 655 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            660                 665                 670 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        675                 680                 685 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    690                 695                 700 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
705                 710                 715                 720 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                725                 730                 735 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu
            740                 745                 750 

Gly Lys
       


<210>    17
<211>    2
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 1


<400>    17
Gly Gly
177    


<210>    18
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 2


<400>    18
Gly Ala Gly
  1        


<210>    19
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 3


<400>    19
Gly Pro Ala
  1        


<210>    20
<211>    1
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 4


<400>    20
Gly
119


<210>    21
<211>    2
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 5


<400>    21
Gly Ser
 11    


<210>    22
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 6


<400>    22
Gly Ser Gly Ser Gly
  1               5


<210>    23
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 7


<400>    23
Gly Gly Ser
  1        


<210>    24
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 8


<400>    24
Gly Gly Ser Ser Gly
  1               5


<210>    25
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 9


<400>    25
Gly Gly Ser Gly
  1            


<210>    26
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 10


<400>    26
Gly Gly Gly Ser
  1            


<210>    27
<211>    6
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 11


<400>    27
Gly Gly Ser Gly Gly Gly
  1               5    


<210>    28
<211>    13
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 12


<400>    28
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
  1               5                  10            
