                         SEQUENCE LISTING

<110>  Flagship Pioneering Innovations VI, LLC
 
<120>  ASPARAGINASE PROTEINS

<130>  5708.1031001

<150>  63/093,974
<151>  2020-10-20

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  326
<212>  PRT
<213>  Escherichia coli

<400>  1

Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asn 
    50                  55                  60                  


Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Thr Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210>  2
<211>  327
<212>  PRT
<213>  Erwinia chrysanthemi

<400>  2

Ala Asp Lys Leu Pro Asn Ile Val Ile Leu Ala Thr Gly Gly Thr Ile 
1               5                   10                  15      


Ala Gly Ser Ala Ala Thr Gly Thr Gln Thr Thr Gly Tyr Lys Ala Gly 
            20                  25                  30          


Ala Leu Gly Val Asp Thr Leu Ile Asn Ala Val Pro Glu Val Lys Lys 
        35                  40                  45              


Leu Ala Asn Val Lys Gly Glu Gln Phe Ser Asn Met Ala Ser Glu Asn 
    50                  55                  60                  


Met Thr Gly Asp Val Val Leu Lys Leu Ser Gln Arg Val Asn Glu Leu 
65                  70                  75                  80  


Leu Ala Arg Asp Asp Val Asp Gly Val Val Ile Thr His Gly Thr Asp 
                85                  90                  95      


Thr Val Glu Glu Ser Ala Tyr Phe Leu His Leu Thr Val Lys Ser Asp 
            100                 105                 110         


Lys Pro Val Val Phe Val Ala Ala Met Arg Pro Ala Thr Ala Ile Ser 
        115                 120                 125             


Ala Asp Gly Pro Met Asn Leu Leu Glu Ala Val Arg Val Ala Gly Asp 
    130                 135                 140                 


Lys Gln Ser Arg Gly Arg Gly Val Met Val Val Leu Asn Asp Arg Ile 
145                 150                 155                 160 


Gly Ser Ala Arg Tyr Ile Thr Lys Thr Asn Ala Ser Thr Leu Asp Thr 
                165                 170                 175     


Phe Lys Ala Asn Glu Glu Gly Tyr Leu Gly Val Ile Ile Gly Asn Arg 
            180                 185                 190         


Ile Tyr Tyr Gln Asn Arg Ile Asp Lys Leu His Thr Thr Arg Ser Val 
        195                 200                 205             


Phe Asp Val Arg Gly Leu Thr Ser Leu Pro Lys Val Asp Ile Leu Tyr 
    210                 215                 220                 


Gly Tyr Gln Asp Asp Pro Glu Tyr Leu Tyr Asp Ala Ala Ile Gln His 
225                 230                 235                 240 


Gly Val Lys Gly Ile Val Tyr Ala Gly Met Gly Ala Gly Ser Val Ser 
                245                 250                 255     


Val Arg Gly Ile Ala Gly Met Arg Lys Ala Met Glu Lys Gly Val Val 
            260                 265                 270         


Val Ile Arg Ser Thr Arg Thr Gly Asn Gly Ile Val Pro Pro Asp Glu 
        275                 280                 285             


Glu Leu Pro Gly Leu Val Ser Asp Ser Leu Asn Pro Ala His Ala Arg 
    290                 295                 300                 


Ile Leu Leu Met Leu Ala Leu Thr Arg Thr Ser Asp Pro Lys Val Ile 
305                 310                 315                 320 


Gln Glu Tyr Phe His Thr Tyr 
                325         


<210>  3
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus asparaginase sequence


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (33)..(34)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(38)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (52)..(52)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (64)..(64)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (68)..(68)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (71)..(72)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (75)..(76)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (78)..(80)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (104)..(104)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (106)..(106)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (138)..(140)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (142)..(144)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (192)..(192)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (196)..(196)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (199)..(202)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (207)..(210)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (224)..(225)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (229)..(229)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (232)..(233)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (252)..(252)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (255)..(256)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (258)..(259)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (262)..(263)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (265)..(265)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (287)..(288)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (311)..(312)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (314)..(314)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (316)..(318)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (321)..(321)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (324)..(325)
<223>  Xaa can be any naturally occurring amino acid

<400>  3

Xaa Xaa Xaa Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Xaa Xaa Leu Xaa Xaa Xaa Val Pro Gln Leu Xaa Asp Ile Ala Xaa Val 
        35                  40                  45              


Xaa Xaa Glu Xaa Val Xaa Asn Ile Gly Ser Gln Asp Met Asn Asp Xaa 
    50                  55                  60                  


Val Trp Leu Xaa Leu Ala Xaa Xaa Ile Asn Xaa Xaa Cys Xaa Xaa Xaa 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Xaa Cys Xaa Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Xaa Xaa Xaa Ser Xaa Xaa Xaa 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Xaa 
            180                 185                 190         


Pro Ala Arg Xaa His Thr Xaa Xaa Xaa Xaa Phe Asp Val Ser Xaa Xaa 
        195                 200                 205             


Xaa Xaa Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Xaa 
    210                 215                 220                 


Xaa Leu Pro Ala Xaa Ala Leu Xaa Xaa Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Xaa Val Phe Xaa Xaa 
                245                 250                 255     


Leu Xaa Xaa Ala Ala Xaa Xaa Gly Xaa Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Xaa Xaa 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Xaa Xaa Thr Xaa Asp Xaa Xaa Xaa Ile Gln 
305                 310                 315                 320 


Xaa Ile Phe Xaa Xaa Tyr 
                325     


<210>  4
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V1

<400>  4

Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Asn 
    50                  55                  60                  


Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Ser Asp Ser Glu Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Ile Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Asp Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Ile Gln Thr Lys Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210>  5
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V2

<400>  5

Leu Lys Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Lys Ala Val Pro Gln Leu Ala Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Lys Lys Ile Asn Asp Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Gln Arg Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Pro His Thr Pro Asp Thr Glu Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Thr Ser Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Val Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Lys Thr 
                245                 250                 255     


Leu Ala Glu Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Asn Asp Pro Glu Glu Ile Gln 
305                 310                 315                 320 


Arg Ile Phe Asp Thr Tyr 
                325     


<210>  6
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V3

<400>  6

Leu Lys Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Asp Lys Leu Val Glu Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Leu Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Pro Ala Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Pro His Thr Leu Ala Thr Glu Phe Asp Val Ser Ser Leu 
        195                 200                 205             


Thr Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Val Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Lys Ala Ala Glu Asp Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Lys Asp Pro Glu Glu Ile Gln 
305                 310                 315                 320 


Lys Ile Phe Asp Thr Tyr 
                325     


<210>  7
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V4

<400>  7

Ile Lys Lys Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Glu Leu Val Glu Arg Val Pro Gln Leu Lys Asp Ile Ala Glu Val 
        35                  40                  45              


Lys Gly Glu Gln Val Met Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Lys Lys Ile Asn Glu Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Gln Arg Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Pro His Thr Pro Asp Thr Glu Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Thr Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Ser Leu Pro Ala Lys Ala Leu Val Glu Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Lys Ala Ala Glu Asp Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Lys Asp Pro Glu Glu Ile Gln 
305                 310                 315                 320 


Arg Ile Phe Asp Thr Tyr 
                325     


<210>  8
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5

<400>  8

Ile Lys Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Lys Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Arg Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Glu Lys Ile Asn Lys Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Gln Arg Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Leu His Thr Pro Leu Thr Glu Phe Asp Val Ser Ser Leu 
        195                 200                 205             


Asp Ala Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Ala Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Gln Thr 
                245                 250                 255     


Leu Ala Gln Ala Ala Ala Asp Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Lys Asp Pro Glu Glu Ile Gln 
305                 310                 315                 320 


Glu Ile Phe Asp Arg Tyr 
                325     


<210>  9
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V6

<400>  9

Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Asp Asn Leu Val Ala Leu Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Lys Lys Ile Asn Asp Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Glu Arg Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Pro Leu Thr Glu Phe Asp Val Ser Asn Leu 
        195                 200                 205             


Thr Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Leu Val Phe Ile Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Glu Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Asn Asp Pro Arg Glu Ile Gln 
305                 310                 315                 320 


Glu Ile Phe Asp Thr Tyr 
                325     


<210>  10
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V7

<400>  10

Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Lys Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Asn 
    50                  55                  60                  


Val Trp Leu Arg Leu Ala Lys Lys Ile Asn Asp Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Arg Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Pro Leu Thr Pro Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Ser Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Asn Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210>  11
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V8

<400>  11

Lys Lys Lys Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Asp Glu Leu Ile Ala Arg Val Pro Gln Leu Ala Asp Ile Ala Glu Val 
        35                  40                  45              


Asp Gly Glu Gln Val Met Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Lys Arg Ile Asn Glu Leu Cys Glu Gln Tyr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Ser Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala His Pro Glu Ser Lys Gly Gln 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Gln 
            180                 185                 190         


Pro Ala Arg Pro His Thr Leu Ala Ser Glu Phe Asp Val Ser Lys Ile 
        195                 200                 205             


Glu Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Phe 
    210                 215                 220                 


Val Leu Pro Ala Glu Ala Leu Ile Lys Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Lys Ala 
                245                 250                 255     


Leu Lys Glu Ala Ala Lys Asn Gly Ile Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Glu Gln 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Val Lys Thr Asn Asp Arg Arg Glu Ile Gln 
305                 310                 315                 320 


Arg Ile Phe Asp Thr Tyr 
                325     


<210>  12
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V9

<400>  12

Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Ala Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Val Glu Gln Val Lys Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Lys Leu Ala Lys Lys Ile Asn Asp Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Arg Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Leu His Thr Leu Glu Thr Glu Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asp Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Val Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Lys Thr 
                245                 250                 255     


Leu Ala Glu Ala Ala Arg Arg Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Glu Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Lys Thr Asn Asp Pro Arg Glu Ile Gln 
305                 310                 315                 320 


Glu Ile Phe Arg Arg Tyr 
                325     


<210>  13
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V10

<400>  13

Met Lys Lys Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Asp Glu Leu Val Ala Gly Val Pro Gln Leu Ala Asp Ile Ala Asp Val 
        35                  40                  45              


Thr Ala Glu Glu Val Arg Asn Ile Gly Ser Gln Asp Met Asn Asp Glu 
    50                  55                  60                  


Val Trp Leu Glu Leu Ala Arg Lys Ile Asn Glu Asp Cys Glu Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Thr Cys Asn Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Asp Ala Ser Ala Gly Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Lys Asp Thr Pro Phe Asp Val Ser Gly Leu 
        195                 200                 205             


Thr Ser Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Ser Leu Pro Ala Lys Ala Leu Val Glu Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Glu Val Phe Glu Thr 
                245                 250                 255     


Leu Ala Glu Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Ala 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Leu Thr Lys Asp Pro Ala Ala Ile Gln 
305                 310                 315                 320 


Ala Ile Phe Asp Glu Tyr 
                325     


<210>  14
<211>  24
<212>  PRT
<213>  Escherichia coli

<400>  14

Met Glu Phe Phe Lys Lys Thr Ala Leu Ala Ala Leu Val Met Gly Phe 
1               5                   10                  15      


Ser Gly Ala Ala Leu Ala Gly Ser 
            20                  


<210>  15
<211>  25
<212>  PRT
<213>  Erwinia chrysanthemi

<400>  15

Met Glu Arg Trp Phe Lys Ser Leu Phe Val Leu Val Leu Phe Phe Val 
1               5                   10                  15      


Phe Thr Ala Ser Ala Ala Asp Gly Ser 
            20                  25  


<210>  16
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Resurfaced

<400>  16

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Gly Ser 
            20                  


<210>  17
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6x His tag

<400>  17

His His His His His His 
1               5       


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV protease cleavage site

<400>  18

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


