                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Novel process

<130>  Case 33828

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  334
<212>  PRT
<213>  Yarrowia lipolytica

<400>  1

Met Lys Leu Ser Thr Ile Leu Phe Thr Ala Cys Ala Thr Leu Ala Ala 
1               5                   10                  15      


Ala Leu Pro Ser Pro Ile Thr Pro Ser Glu Ala Ala Val Leu Gln Lys 
            20                  25                  30          


Arg Val Tyr Thr Ser Thr Glu Thr Ser His Ile Asp Gln Glu Ser Tyr 
        35                  40                  45              


Asn Phe Phe Glu Lys Tyr Ala Arg Leu Ala Asn Ile Gly Tyr Cys Val 
    50                  55                  60                  


Gly Pro Gly Thr Lys Ile Phe Lys Pro Phe Asn Cys Gly Leu Gln Cys 
65                  70                  75                  80  


Ala His Phe Pro Asn Val Glu Leu Ile Glu Glu Phe His Asp Pro Arg 
                85                  90                  95      


Leu Ile Phe Asp Val Ser Gly Tyr Leu Ala Val Asp His Ala Ser Lys 
            100                 105                 110         


Gln Ile Tyr Leu Val Ile Arg Gly Thr His Ser Leu Glu Asp Val Ile 
        115                 120                 125             


Thr Asp Ile Arg Ile Met Gln Ala Pro Leu Thr Asn Phe Asp Leu Ala 
    130                 135                 140                 


Ala Asn Ile Ser Ser Thr Ala Thr Cys Asp Asp Cys Leu Val His Asn 
145                 150                 155                 160 


Gly Phe Ile Gln Ser Tyr Asn Asn Thr Tyr Asn Gln Ile Gly Pro Lys 
                165                 170                 175     


Leu Asp Ser Val Ile Glu Gln Tyr Pro Asp Tyr Gln Ile Ala Val Thr 
            180                 185                 190         


Gly His Ser Leu Gly Gly Ala Ala Ala Leu Leu Phe Gly Ile Asn Leu 
        195                 200                 205             


Lys Val Asn Gly His Asp Pro Leu Val Val Thr Leu Gly Gln Pro Ile 
    210                 215                 220                 


Val Gly Asn Ala Gly Phe Ala Asn Trp Val Asp Lys Leu Phe Phe Gly 
225                 230                 235                 240 


Gln Glu Asn Pro Asp Val Ser Lys Val Ser Lys Asp Arg Lys Leu Tyr 
                245                 250                 255     


Arg Ile Thr His Arg Gly Asp Ile Val Pro Gln Val Pro Phe Trp Asp 
            260                 265                 270         


Gly Tyr Gln His Cys Ser Gly Glu Val Phe Ile Asp Trp Pro Leu Ile 
        275                 280                 285             


His Pro Pro Leu Ser Asn Val Val Met Cys Gln Gly Gln Ser Asn Lys 
    290                 295                 300                 


Gln Cys Ser Ala Gly Asn Thr Leu Leu Gln Gln Val Asn Val Ile Gly 
305                 310                 315                 320 


Asn His Leu Gln Tyr Phe Val Thr Glu Gly Val Cys Gly Ile 
                325                 330                 


<210>  2
<211>  1005
<212>  DNA
<213>  Yarrowia lipolytica

<400>  2
atgaagcttt ccaccatcct cttcacagcc tgcgctaccc tggctgccgc cctcccttcc       60

cccatcactc cttctgaggc cgcagttctc cagaagcgag tgtacacctc taccgagacc      120

tctcacattg accaggagtc ctacaacttc tttgagaagt acgcccgact cgcaaacatt      180

ggatattgtg ttggtcccgg cactaagatc ttcaagccct tcaactgtgg cctgcaatgt      240

gcccacttcc ccaacgttga gctcatcgag gagttccacg acccccgtct catctttgat      300

gtttctggtt acctcgctgt tgatcatgcc tccaagcaga tctaccttgt tattcgagga      360

acccactctc tggaggacgt cataaccgac atccgaatca tgcaggctcc tctgacgaac      420

tttgatcttg ctgctaacat ctcttctact gctacttgtg atgactgtct tgtccacaat      480

ggcttcatcc agtcctacaa caacacctac aatcagatcg gccccaagct cgactctgtg      540

attgagcagt atcccgacta ccagattgct gtcaccggtc actctctcgg aggagctgca      600

gcccttctgt tcggaatcaa cctcaaggtt aacggccacg atcccctcgt tgttactctt      660

ggtcagccca ttgtcggtaa cgctggcttt gctaactggg tcgataaact cttctttggc      720

caggagaacc ccgatgtctc caaggtgtcc aaagaccgaa agctctaccg aatcacccac      780

cgaggagata tcgtccctca agtgcccttc tgggacggtt accagcactg ctctggtgag      840

gtctttattg actggcccct gatccaccct cctctctcca acgttgtcat gtgccagggc      900

cagagcaata aacagtgctc tgccggtaac actctgctcc agcaggtcaa tgtgattgga      960

aaccatctgc agtacttcgt caccgagggt gtctgtggta tctaa                     1005


<210>  3
<211>  498
<212>  PRT
<213>  Yarrowia lipolytica

<400>  3

Met Pro Leu Glu Leu Pro Ser Leu Asn Ala Ser Ile Val Gly Asn Thr 
1               5                   10                  15      


Val Gln Asn Gly Ala Val Glu Gln Phe Leu Asn Ile Arg Tyr Ala Asp 
            20                  25                  30          


Ile Pro Gly Lys Phe Glu Lys Pro Val Leu Lys Asn Asp Trp Asn Gly 
        35                  40                  45              


Ala Glu Ile Asp Ala Thr Lys Val Gly Pro Val Cys Pro Gln Pro Arg 
    50                  55                  60                  


Thr Pro Phe Asn Phe Phe Ser Val Pro Asp Asp Leu Trp Glu Lys Val 
65                  70                  75                  80  


Asn Val Asp Thr Tyr Gln Asp Gly Leu Leu Cys Asp Asn Leu Ile Val 
                85                  90                  95      


Thr Arg Pro Lys Gly Val Ser Ala Asn Ala Arg Leu Pro Thr Val Val 
            100                 105                 110         


Trp Ile His Gly Gly Ser Asn Ile Glu Gly Ser Ile Tyr Asn Leu Ile 
        115                 120                 125             


Tyr Glu Pro Gln Phe Leu Val Ala Glu Ser Val Arg Val Gly Lys Pro 
    130                 135                 140                 


Ile Val His Val Cys Ile Glu Tyr Arg Leu Gly Leu Ala Gly Phe Leu 
145                 150                 155                 160 


Thr Lys Asn Gly Lys Gly Asn Trp Gly Thr Trp Asp Gln Tyr Thr Gly 
                165                 170                 175     


Cys Gln Trp Val Asn Arg His Ile Gln Asp Phe Gly Gly Asp Pro Leu 
            180                 185                 190         


Asn Val Thr Leu Thr Gly Glu Ser Ala Gly Ser Val Ala Val His Asn 
        195                 200                 205             


Met Leu Ile Lys Asp Ser Met Asn Gly Arg Lys Leu Phe Arg Asn Ala 
    210                 215                 220                 


Val Met Met Ser Gly Thr Leu Glu Thr Ile Thr Pro Gln Pro Pro Lys 
225                 230                 235                 240 


Trp His Ala Arg Leu Glu Glu Lys Val Ala Lys Val Thr Gly Lys Glu 
                245                 250                 255     


Val Ala Asp Leu Ala Ser Leu Ser Asp Lys Glu Leu Leu Asp Ala Gln 
            260                 265                 270         


Ile Lys Leu Asn Val Ala Val Cys Met Thr Cys Asp Asp Gly Asp Phe 
        275                 280                 285             


Phe Glu Pro Gly Trp Lys Gln His Leu Thr Pro Asp Trp Leu Asp Lys 
    290                 295                 300                 


Leu Ile Ile Ser Asp Cys Lys Asp Glu Gly Met Leu Tyr Phe Leu Pro 
305                 310                 315                 320 


Val Asn Ala Gln Asp Asp Glu Glu Leu Leu Ala Lys Val Ala Lys Ser 
                325                 330                 335     


Pro Val Gly Lys Glu Ile Ser Glu Leu Tyr Gly Ile Lys Glu Gly Gly 
            340                 345                 350         


Asp Ile Lys Ser Ala Cys Leu Asp Leu Lys Thr Asp Ala Thr Phe Asn 
        355                 360                 365             


Tyr Phe Asn His Leu Leu Phe Lys Lys Met Glu Glu Ala Arg Asn Asn 
    370                 375                 380                 


Gly Ser Thr Ser Arg Val Tyr Arg Leu Ala Val Asp Glu Pro Asn Pro 
385                 390                 395                 400 


His Asn Pro Asp Gln Arg Ala His His Ala Val Asp Val Leu Tyr Met 
                405                 410                 415     


Phe Asn Ser Thr Lys Phe Asn Glu His Gly Asp Lys Leu Ser Arg Leu 
            420                 425                 430         


Phe Gln Ser His Phe Leu Arg Leu Ala Tyr Gly Leu Glu Pro Trp Asp 
        435                 440                 445             


His Arg Asn Phe Gly Val Tyr Arg Asn Gly Gly Tyr Gln Gln Leu Pro 
    450                 455                 460                 


Leu Ser Glu Leu Asn Lys Val Arg Pro Val Glu Arg Tyr Glu Ala Leu 
465                 470                 475                 480 


Ser Lys Met Asp Phe Gly Gln Val Gly Arg Leu Ser Asn Ala Leu Ser 
                485                 490                 495     


Arg Leu 
        


<210>  4
<211>  1497
<212>  DNA
<213>  Yarrowia lipolytica

<400>  4
atgcctctcg aactcccctc gctcaacgcc tcgattgtcg gcaacaccgt tcagaacggc       60

gctgttgagc agtttctcaa catccgatac gccgacattc ctggcaagtt tgagaagccc      120

gtgctcaaga acgattggaa cggcgcggag atcgacgcca ccaaggtcgg tcccgtgtgc      180

ccccaacccc gcaccccatt caacttcttc tccgtgccag acgacctctg ggagaaagtc      240

aatgtggaca cgtaccagga cggtctgctg tgcgacaacc tgattgtgac gcgaccgaag      300

ggcgtgtctg ccaacgcccg gctgcccact gttgtgtgga tccacggcgg ctccaatatt      360

gagggcagta tctacaacct catctatgag ccccagttcc tggtggcaga gtcggtgcga      420

gtaggcaagc cgattgtgca cgtgtgtatc gagtaccgac tgggtctcgc gggcttcctc      480

accaagaacg gcaagggcaa ctggggcacg tgggatcagt acacgggctg ccagtgggtc      540

aaccgccaca ttcaggactt tggaggcgat cctttgaacg tgacattgac cggtgagtct      600

gccggctctg tagcagtcca taacatgctc atcaaggact ccatgaacgg ccgaaagttg      660

ttccgaaatg ccgtcatgat gagtggcact ctcgagacca tcactcctca gcctcccaag      720

tggcatgctc gtttggagga gaaggtggcc aaggtcactg gcaaggaagt ggccgacctt      780

gcttctctgt ccgataagga gctgctcgac gcccagatca agctcaatgt ggctgtgtgc      840

atgacttgcg acgacggcga ctttttcgag cccggatgga agcagcatct gactcctgac      900

tggctcgaca agctcatcat ctccgattgc aaggacgagg gcatgctgta tttcctgcca      960

gtcaacgcgc aggacgacga ggagctgttg gcaaaggtgg ccaagtcgcc cgtgggtaag     1020

gagatttccg agctttacgg catcaaggag ggtggcgata tcaagtctgc gtgtctcgat     1080

ctcaagactg acgccacctt caattacttt aaccatctgc tgttcaagaa gatggaggag     1140

gcccgaaaca acggctccac ttctcgagtt taccgtctgg ccgtcgatga gcccaacccc     1200

cacaaccccg accagcgggc ccaccacgcc gtcgacgtgc tgtacatgtt caactcgacc     1260

aagttcaacg agcacggcga caagctgtct cggctgttcc agagccactt tttgcggctg     1320

gcgtatggcc tggagccctg ggaccatcga aactttggag tgtacagaaa cggcggctac     1380

cagcagctgc cgctgagtga gttgaacaag gtccgacccg tcgagcggta cgaggcgctg     1440

tccaagatgg actttggcca ggttgggcgt ttgtccaatg cgctttcgcg cctatga        1497


<210>  5
<211>  371
<212>  PRT
<213>  Yarrowia lipolytica

<400>  5

Met Val Ser Leu Ser Ala Arg Ile Lys Asp Phe Phe Ser Val Leu Leu 
1               5                   10                  15      


Leu Gly Ala Ala Thr Ile Thr Pro Ser Thr Gln Thr Ala Gly Val Ser 
            20                  25                  30          


Gln Gly Phe Tyr Asp Phe Ala Arg Asp Phe Ala His Leu Ser Asn Ile 
        35                  40                  45              


Ala Tyr Cys Val Asn Ala Pro Ile Thr Pro Leu Asn Pro Asp Phe Thr 
    50                  55                  60                  


Cys Gly Asn Ser Cys Lys His Phe Pro Glu Ile Glu Leu Val Lys Thr 
65                  70                  75                  80  


Phe Gly Gly Asn Phe Phe Lys Thr Ser Ile Thr Gly Tyr Leu Ala Val 
                85                  90                  95      


Asp His Val Lys Lys Glu Lys Tyr Val Val Phe Arg Gly Thr Phe Ser 
            100                 105                 110         


Leu Ala Asp Ala Ile Thr Asp Met Gln Phe Gln Leu Ser Pro Phe Leu 
        115                 120                 125             


Val Asp Val Pro Ala Leu Asn Thr Phe Ser Ala Asn Asp Thr Thr Ala 
    130                 135                 140                 


Glu Ala Gln Thr His Cys Glu Gly Cys Lys Ile His Asp Gly Phe Ser 
145                 150                 155                 160 


Lys Ala Phe Thr Glu Thr Trp Gly Asn Ile Gly Glu Asp Leu Gln Lys 
                165                 170                 175     


His Leu Asp Ala Asn Pro Asp Tyr Gln Leu Tyr Val Thr Gly His Ser 
            180                 185                 190         


Leu Gly Ala Ala Met Ala Leu Leu Gly Ala Thr Ser Ile Lys Leu Lys 
        195                 200                 205             


Gly Tyr Asp Pro Ile Leu Ile Asn Tyr Gly Gln Pro Arg Val Gly Asn 
    210                 215                 220                 


Lys Pro Phe Ala Glu Phe Ile Asn Lys Leu Trp Phe Gly Glu Gly Asn 
225                 230                 235                 240 


Gly Leu Glu Ile Thr Pro Glu Arg Lys Leu Tyr Arg Met Thr His Trp 
                245                 250                 255     


Asn Asp Ile Phe Val Gly Leu Pro Asn Trp Glu Gly Tyr Thr His Ser 
            260                 265                 270         


Asn Gly Glu Val Tyr Ile Asn Asn Arg Phe Ile Asn Pro Pro Leu Lys 
        275                 280                 285             


Asp Val Ile Ser Cys Ala Gly Gly Glu Asn Ser Lys Cys Tyr Arg Ser 
    290                 295                 300                 


Ser Phe Ser Leu Leu Ser Gln Ile Asn Leu Leu Gln Asn His Leu Ala 
305                 310                 315                 320 


Tyr Ile Asp Tyr Ile Gly Tyr Cys Ala Leu Asn Ile Gly Arg Arg Glu 
                325                 330                 335     


Leu Ala Asp Gln Glu His Tyr Thr Gly Pro Tyr Tyr Tyr Gly His Arg 
            340                 345                 350         


Ser Glu Glu Asp Phe Lys Lys Leu Gly Leu Glu Leu Ser Thr Pro Gln 
        355                 360                 365             


Val Glu Asn 
    370     


<210>  6
<211>  1116
<212>  DNA
<213>  Yarrowia lipolytica

<400>  6
atggtatccc tctctgctcg aatcaaagac tttttttcgg tcctcctcct cggagctgca       60

accatcactc cctccacaca gaccgcaggc gtgtctcaag ggttctatga ttttgctcgg      120

gactttgccc atctgtccaa cattgcctac tgtgtcaatg ctcccatcac tccactgaac      180

ccggacttca cctgtggcaa ctcgtgcaag cactttccgg aaattgagct tgtgaagaca      240

tttggaggca acttcttcaa gacctccatt acgggctacc tggctgtcga tcatgtcaag      300

aaggagaagt acgttgtctt ccgaggaacc ttttcgctgg cagacgcgat cacggacatg      360

cagttccagc tgtctccttt cctggtcgat gtgcctgccc tgaacacttt ctcagctaat      420

gacaccaccg cagaggccca gacgcactgt gagggctgca aaattcacga cggcttctcc      480

aaggccttta ccgagacctg gggtaacatt ggtgaggatc tgcagaaaca cctggacgct      540

aacccggact accagctgta cgtgactggc cattctctgg gagctgctat ggcccttctt      600

ggagctactt ccatcaagct caagggctac gatcccattc tcatcaacta cggacagccc      660

cgagtcggaa acaagccctt cgctgagttc attaacaagt tgtggtttgg agaaggcaac      720

ggtctggaaa tcacccccga gagaaagctg taccgaatga cccactggaa cgacatcttt      780

gttggcctgc ccaactggga gggatacacc cactctaacg gtgaagtata catcaacaac      840

cggttcatca accctcctct caaggatgtc atctcttgtg ctggaggcga aaactcgaag      900

tgctaccgat cctcgttcag cctgctgtcc cagatcaatc tgctccaaaa ccacctggct      960

tacattgatt acattggata ctgcgctctg aacattggtc gacgagagct tgccgatcag     1020

gaacattaca ctggtcctta ttactatggt catcgatctg aggaggactt taagaagttg     1080

ggcttggagc tatccacccc acaagttgag aactga                               1116


<210>  7
<211>  406
<212>  PRT
<213>  Yarrowia lipolytica

<400>  7

Met Ala Gly Phe Asn Phe Thr Phe Gly Gln Val Ile Ser Tyr Leu Ala 
1               5                   10                  15      


Ser Met Leu Tyr Gly Gln Val Asp Ala Thr Ser Ser Ser Thr Arg Ile 
            20                  25                  30          


Gln Ala Thr Gln Asp Leu Tyr Asp Phe Thr Ala Lys Phe Ser Arg Leu 
        35                  40                  45              


Ser Asn Ile Ala Tyr Cys Ile Asn Ala Pro Phe Thr Pro Leu Arg Thr 
    50                  55                  60                  


Asp Phe Thr Cys Gly Glu Ser Cys Arg Tyr Phe Pro Asp Leu Gln Leu 
65                  70                  75                  80  


Asp Ser Val Phe Gly Gly Asn Phe Ser Ser Ala Ser Thr Thr Gly Tyr 
                85                  90                  95      


Ile Ala Tyr Asp His Lys Lys Lys Glu Lys Tyr Ile Val Phe Arg Gly 
            100                 105                 110         


Thr Phe Ser Ile Pro Asp Ile Ile Thr Asp Ile Gln Phe Gln Thr Ala 
        115                 120                 125             


Pro Trp Leu Thr Ser Leu Pro Thr His Leu Ile Pro Thr Lys Glu Asp 
    130                 135                 140                 


Phe Glu His Lys Gln Ala Ile Leu Lys His Tyr Ala Ala Glu Asn Lys 
145                 150                 155                 160 


Gly Leu Ser Asn Leu Glu Glu Arg Gln Asp Val Val His Glu Asp Pro 
                165                 170                 175     


Ser Leu Val Pro Lys Lys Met Asp Lys Cys Glu Asn Cys Gln Ile His 
            180                 185                 190         


Asp Gly Phe Ala Lys Gly Phe Asn Glu Thr Ile Glu His Ala Gly Pro 
        195                 200                 205             


Gln Ile Glu Lys Phe Leu Gly Asn Asn Thr Asp Tyr Lys Met Phe Val 
    210                 215                 220                 


Val Gly His Ser Leu Gly Ala Ala Gln Ala Gln Leu Phe Ala Thr Gln 
225                 230                 235                 240 


Phe Lys Leu Leu Gly Phe Asp Pro Tyr Met Ile Asn Phe Gly Gln Pro 
                245                 250                 255     


Arg Leu Gly Asn Pro Glu Phe Ala Ala Tyr Ile Asn Gln Leu Trp Phe 
            260                 265                 270         


Asn Asp Thr Gly Leu Val Val Asn Asp Ala Arg Arg Phe Tyr Arg Val 
        275                 280                 285             


Thr His Trp Asn Asp Ile Val Val Gly Val Pro Asp Trp Leu Asn Tyr 
    290                 295                 300                 


Thr His Ser Ile Gly Glu Val Phe Ile Asp Glu Glu Ser Val Tyr Pro 
305                 310                 315                 320 


Lys Leu Asp Lys Val Val Val Cys Glu Gly Gly Glu Asn Pro Leu Cys 
                325                 330                 335     


His Arg Gly Thr Phe Asn Leu Trp Ser Arg Ile Asn Phe Leu Gln Asn 
            340                 345                 350         


His Leu Ala Tyr Ile Phe Tyr Ile Gly Leu Cys Ala Phe Asn Ile Gly 
        355                 360                 365             


Arg Arg Asp Val Leu Asn Met Pro Gln Tyr Gln Gly Asn Phe Ser Tyr 
    370                 375                 380                 


Gln His Asn Ile Asp Pro Asn Tyr Asn Tyr Asp Thr Lys Val Pro Thr 
385                 390                 395                 400 


Arg Ile Ser Lys Ser Asn 
                405     


<210>  8
<211>  1221
<212>  DNA
<213>  Yarrowia lipolytica

<400>  8
atggcagggt tcaacttcac tttcgggcag gtgatttcgt acctggcttc catgctctat       60

ggacaggtgg atgccacttc atcctccact cgaatccagg ccacccagga tctgtacgac      120

ttcacagcca agttttcgcg actctcaaac atcgcgtact gcatcaatgc ccccttcacg      180

cctctcagaa cggacttcac ctgcggagaa agctgtcggt acttccccga cctccagctg      240

gactcagtgt ttggtggtaa cttctcctca gcctccacta ccggctacat tgcatacgac      300

cacaagaaga aggaaaagta cattgtgttt cgaggaactt tcagtatccc tgatatcatc      360

acagacattc aatttcagac tgccccttgg ttgacctctc tgcccacgca tctgatccct      420

accaaggagg actttgaaca caagcaggcc atcctgaagc actacgctgc cgaaaacaag      480

ggtctcagca acctggaaga gcgacaggat gttgtgcatg aagaccctag cctggttccc      540

aagaaaatgg acaagtgcga gaactgccag atccatgacg gattcgccaa gggcttcaac      600

gagactatcg agcatgccgg accccagatt gaaaagttcc tgggcaataa caccgactac      660

aagatgtttg ttgttggcca ctctctagga gctgctcagg cccagctgtt tgctacacag      720

ttcaaactgc tgggatttga cccttacatg atcaactttg gacagcctcg acttggaaac      780

cctgagttcg ccgcctacat caaccagctg tggttcaacg atactggtct ggttgtcaat      840

gatgcccgac gattctaccg agtgactcac tggaacgata tcgtcgtggg agtgcccgac      900

tggctcaatt acacccactc tatcggagag gtgttcatag acgaggagag cgtttacccc      960

aagctggaca aggtggtggt gtgcgaggga ggagagaacc ccctgtgcca ccgaggaact     1020

ttcaacctgt ggtcacgaat caacttcctg cagaaccatt tggcttatat cttctacatt     1080

ggtctgtgtg ctttcaacat tggccgaaga gacgtgctca acatgccaca ataccagggc     1140

aacttctcgt accagcacaa catcgacccc aactacaatt acgataccaa ggttcccacc     1200

cggatcagta aatcaaacta a                                               1221


<210>  9
<211>  11606
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression plasmid

<400>  9
cgcgtggatc gccggtgcgt tgacgttggt gacctccagc cagaggtgcc cggcgccccg       60

ctcgccggcg aactccgtcg cgagccccat caacgcgcgc ccgaccccgt gcccccggtg      120

ctccggggcg acctcgatgt cctcgacggt cagccggcgg ttccacgccg agtacgagat      180

gaccacgaag cccgccaggt cgccgtcgtc cccgtacgcg acgaacgtcc gggagtccgg      240

gtcgccgtcc tccccgtcgt ccgattcgtc gtccgattcg tcgtcgggga acaccttggt      300

caggggcggg tccaccggca cctcccgcag ggtgaagccg tccccggtgg cggtgacgcg      360

gaagacggtg tcggtggtga aggacccatc cagtgcctcg atggcctcgg cgtcccccgg      420

gacactggtg cggtaccggt aagccgtgtc gtcaagagtg gtcatttttg tgtctaggtg      480

tttgtgtttg gactgcgatc agtgaagaaa agaagaggaa aaattgtgca agaaattttg      540

ctttcaagac ttggctgatg cagcagggta actctgggac acagacctat gtttgtggtt      600

aaactcaatg cacgtggtac gtgcgtggag cgcttaccca tccaagggtg tggacatgga      660

accgacggtc cgtggagttg tgtaatgtca ttttggcgac tcttgaagca aggctataaa      720

aaaattgtgt ggcttgagtc ttatcgagct cggtcactac aagagttaat cttcctgtct      780

caggcagaca ggtcaggcag ggttactttt gggtgtgctg taactcactg tatggccgtt      840

agtgcgcata gacgttgtac atactggacc gaattgtagc gtgctcaata gggccaataa      900

agctattgta gggatccgaa ttttcagaac ctaatttatc tgttacccgg cctgtggctc      960

gcacagctta aaaatggtca aactttcccc ttcttgtctt tttttcctca cattcatcag     1020

gttcttgtct tgatctttca agtgagtatt aattaccgac cttggttctt cattgggaga     1080

gcattggaag ccgtggtgca gcaaccacaa aacggttctt ccccttcgat accttcttgc     1140

ctgcctttca atacaagtcg gctcgattag cggtggtcgc ccccgccagc ggagaacatg     1200

gaactaaccc agaatgagag ctaagtggag aaagaagaga gtcagacgac tcaagcgaaa     1260

gcgccgcaag gtccgagctc gatccaaata agcggttttt aacggagatt taacactaaa     1320

tcgaagaact tttcccgttt catttgcgaa tgagctcgtt aacaaaatcc cccagttttt     1380

ttatccagct gtaaggattg acattagtaa tgaattattg tttggtatat ttaaatctgt     1440

agttcctttc tgtccgtgtc ggcaactgtc gtactcgtga tttacttgta ttgacgaata     1500

cttactgtag cgcactctgc tgctactggt cgtaaggatg tgctatttcg gtgtatggtg     1560

ggttttttgg gggtcggaac cgaagactgt tacacgggca cggctcgttg tgtacacgca     1620

cagagctctt gcgagtcatg ttgtagctag ctcgtcgtgt tcaggaactg ttcgatggtt     1680

cggagagagt cgccgcccag aacatacgcg caccgatgtc agcagacagc cttattacaa     1740

gtatattcaa gcaagtatat ccgtagggtg cgggtgattt ggatctaagg ttcgtactca     1800

acactcacga gcagcttgcc tatgttacat ccttttatca gacataacat aattggagtt     1860

tacttacaca cggggtgtac ctgtatgagc accacctaca attgtagcac tggtacttgt     1920

acaaagaatt tattcgtacg aatcacaggg acggccgccc tcaccgaacc agcgaatacc     1980

tcagcggtcc cctgcagtga ctcaacaaag cgatatgaac atcttgcgat ggtatcctgc     2040

tgatagtttt tactgtacaa acacctgtgt agctccttct agcattttta agttattcac     2100

acctcaaggg gagggataaa ttaaataaat tccaaaagcg aagatcgaga aactaaatta     2160

aaattccaaa aacgaagttg gaacacaacc ccccgaaaaa aaacaacaaa caaaaaaccc     2220

aacaaaataa acaaaaacaa aataaatata taactaccag tatctgacta aaagttcaaa     2280

tactcgtact tacaacaaat agaaatgagc cggccaaaat tctgcagaaa aaaatttcaa     2340

acaagtactg gtataattaa attaaaaaac acatcaaagt atcataacgt tagttatttt     2400

attttattta ataaaagaaa acaacaagat gggctcaaaa ctttcaactt atacgataca     2460

taccaaataa caatttagta tttatctaag tgcttttcgt agataatgga atacaaatgg     2520

atatccagag tatacacatg gatagtatac actgacacga caattctgta tctctttatg     2580

ttaactactg tgaggcatta aatagagctt gatatataaa atgttacatt tcacagtctg     2640

aacttttgca gattacctaa tttggtaaga tattaattat gaactgaaag ttgatggcat     2700

ccctaaattt gatgaaagat gaaattgtaa atgaggtggt aaaagagcta cagtcgtttt     2760

gttttgagat accatcatct ctaacgaaat atctattaaa aatctcagtg tgatcatgag     2820

tcattgccat cctggaaaat gtcatcatgg ctgatatttc taactgttta cttgagataa     2880

atatatattt acaagaactt cccttgaaat taatttagat ataaaatgtt tgcgggcaag     2940

ttactacgag gaataaatta tatctagagg ttccgcttcc tcgctcactg actcgctgcg     3000

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     3060

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     3120

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     3180

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     3240

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     3300

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     3360

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     3420

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     3480

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     3540

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt     3600

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     3660

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     3720

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     3780

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     3840

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     3900

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     3960

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     4020

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     4080

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     4140

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     4200

tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat     4260

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     4320

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     4380

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     4440

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     4500

accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt     4560

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     4620

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     4680

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     4740

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     4800

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     4860

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat     4920

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tggcctagga     4980

agcgacttcc aatcgctttg catatccagt accacaccca caggcgtttg tgctactcta     5040

ctgatagcaa tagatgcgtc ataattggtt ggcccgctga gcctccacag gatactattg     5100

cacataccct ggtcatgtgc agatcagctc atttgtggag actctggagt aacttagacg     5160

acgcctggtt caattgccgc aatgtgcgcc cacgcagata atgtattgag gggtggagcg     5220

cctcttgggg acttgctgta cttgtacggg atattaaacg cactcagcaa gaccatgacg     5280

taaaacacac ctactgtacg atacgtactg taggtattgt actcgtaccc ggtactacaa     5340

atagtacgat actatacgga gtgtatttgt accttgatat acgactggcg gagtgaagag     5400

aaggagttga acaagaccag atggggatat cagccccagt gctttgtatt acaagtacga     5460

gtacttaata gatactgtaa ggctattgat acggatggca gtaagtcatt gagtaagcaa     5520

ttgtggccca gcatctcccc tacgtacttg taccataccc catggagaca ccaatggtct     5580

ttcacgcaca ctgtcgtgtg ctgtatcgca gaatcgggtg tccaaccaaa tgccgttacc     5640

cccacgtcac agccgataga cagatacacc atcaatacca gcaggttgta tcatgcggtt     5700

ggctgaaggt aagctgattg gtctaaaaac tgtagctgtc ctaattcaac gagcgctatt     5760

tggggccaac cacctcggcc aagcggcctt taatctgcgt gccccagagg cgtctaatga     5820

ggctctggcc gccactgtag gagtgtttct ctgtgcgcac acgcagtttt gagtttgggc     5880

gactttccct ttttcccaat tgcgtacaca cacagctccg agctaagcgc tgtccttgaa     5940

ccttctccct cttttccctc tttttctctt ccccttcccc tcctccacat taaggccaaa     6000

tcctgaattg caccaactag tacaacgaca acaatggaca agaagtactc catcggtttg     6060

gacattggta ctaactctgt cggctgggcc gtcatcaccg acgagtacaa ggttccctcc     6120

aagaagttca aggtccttgg caacaccgac cgacactcta tcaagaagaa cctgatcggt     6180

gctctgctgt tcgactctgg cgagactgcc gaggccaccc gactgaagcg aaccgctcga     6240

cgccgataca cccgacgaaa gaaccgaatc tgttacctcc aggagatctt cagcaacgag     6300

atggctaagg tcgacgactc cttcttccac cgactcgagg agtctttcct ggtcgaagag     6360

gataagaagc acgagcgaca ccccatcttc ggcaacattg ttgatgaggt tgcctaccat     6420

gagaagtacc ccaccatcta ccacctccga aagaagctcg tcgactccac tgacaaggct     6480

gacctccgac tcatctacct tgctctcgcc cacatgatca agttccgagg tcacttcctc     6540

attgagggtg atctcaaccc cgacaactcc gacgttgaca agctgttcat ccagctcgtc     6600

cagacctaca accagctctt tgaggagaac cctatcaacg cttctggtgt tgacgccaag     6660

gccattctct ccgcccgact ctctaagtcc cgacgactcg agaacctcat tgcccagctg     6720

cccggcgaga agaagaacgg cctcttcggt aacctgattg ctctctctct tggtctgacc     6780

cccaacttca agtccaactt tgacctcgcc gaggacgcca agctccagct gtccaaggac     6840

acctacgatg acgatctgga caacctcctg gcccagatcg gtgaccagta cgccgatctc     6900

ttccttgccg ccaagaacct ctccgacgcc atcctgctct ccgacatcct ccgagtcaac     6960

accgagatta ccaaggctcc tctgtctgcc tctatgatca agcgatacga cgagcaccac     7020

caggatctca ctcttctcaa ggctctcgtc cgacagcagc tccccgagaa gtacaaggag     7080

attttctttg accagtccaa gaacggttac gctggctaca ttgacggtgg tgcttcccag     7140

gaagagtttt acaagttcat caagcctatt ctggagaaga tggacggtac cgaggagctg     7200

ctcgtcaagc tcaaccgaga ggacctcctt cgaaagcagc gaaccttcga taacggctcc     7260

atcccccacc agatccacct gggtgagctc cacgccattc tccgaagaca agaggacttc     7320

taccccttcc taaaggataa ccgagagaag atcgagaaga ttctcacctt ccgaatcccc     7380

tactacgtcg gtcccctcgc tcgaggtaac tcccgatttg cttggatgac ccgaaagtcc     7440

gaggagacta tcaccccctg gaactttgaa gaggtagtcg acaagggtgc ctccgcccag     7500

tctttcattg agcggatgac caacttcgat aagaacctcc ccaacgagaa ggtccttccc     7560

aagcactctc tcctctacga gtacttcacc gtctacaacg agctgaccaa ggtcaagtac     7620

gttaccgagg gcatgcgaaa gcccgctttc ctctctggtg agcagaagaa ggccattgtc     7680

gacctcctgt tcaagactaa ccgaaaagtc accgtcaagc agctcaagga agactacttc     7740

aagaagattg agtgcttcga ctccgtcgag atttccggtg tcgaggaccg attcaacgcc     7800

tccctcggca cctaccacga tcttctgaag atcatcaagg acaaggactt tcttgataac     7860

gaggagaacg aggacattct cgaggacatc gtcctcaccc tcaccctttt cgaggatcga     7920

gagatgatcg aggagcgact caagacctac gcccatctct tcgacgacaa ggtcatgaag     7980

caactcaagc gacgacgata cactggctgg ggccgacttt cccgaaagct catcaacggc     8040

atccgagaca agcagtctgg caagaccatc ctggacttcc tgaagtccga cggtttcgcc     8100

aaccgaaact tcatgcagct catccacgac gactctctta ccttcaaaga ggatatccag     8160

aaggcccagg tttctggcca gggcgactcc ctccacgagc acattgccaa cctcgccgga     8220

tcccccgcca tcaaaaaggg tatcctccag accgtcaagg ttgtcgacga actcgtgaag     8280

gtcatgggcc gacacaagcc cgagaacatc gttatcgaga tggcccgaga gaaccagacc     8340

acccagaagg gtcagaagaa ctcccgagag cgaatgaagc gaatcgaaga gggtatcaag     8400

gagctcggtt cccagattct caaggagcac cccgtcgaga acacccagct ccagaacgag     8460

aaactctacc tgtactacct ccagaatggc cgagacatgt acgttgacca ggagctcgac     8520

atcaaccgac tctccgacta cgacgtcgac cacattgttc ctcagtcctt cctcaaggac     8580

gactccatcg acaacaaggt tctgacccga tctgacaaga accgaggtaa gtccgacaac     8640

gttccctccg aagaggtcgt taagaagatg aagaactact ggcgacagct tctcaacgcc     8700

aaactgatca cccagcgaaa gtttgacaac ctcaccaagg ccgagcgagg tggtctgtcc     8760

gagctggaca aggccggctt cattaagcga cagctggtcg agactcgaca gatcaccaag     8820

cacgtcgccc agatcctcga ctcccgaatg aacaccaagt acgacgagaa cgacaagctc     8880

atccgggagg tcaaggtcat caccctgaag tctaagcttg tctccgactt ccgaaaggac     8940

ttccagttct acaaggtccg agagatcaac aactaccacc acgcccacga cgcctacctc     9000

aacgccgttg ttggtaccgc cctcatcaag aagtatccca agctcgagtc cgagttcgtt     9060

tacggcgact acaaggttta cgatgtccga aagatgattg ccaagtccga gcaggagatc     9120

ggtaaggcca ccgccaagta ctttttctac tccaacatca tgaatttctt caagaccgag     9180

atcactctcg ccaacggtga gattcgaaag cgacccctga ttgagactaa tggtgagact     9240

ggtgagatcg tctgggataa gggccgagac ttcgccaccg tccgaaaggt cctgtccatg     9300

ccccaggtca acattgtcaa gaagaccgag gtccagaccg gtggcttctc caaggagtcc     9360

attctcccca agcgaaactc cgacaaactc atcgcccgta agaaggactg ggatccgaag     9420

aagtacggtg gtttcgattc tcccaccgtt gcctactccg tcctcgttgt tgctaaagtc     9480

gagaagggta agtctaagaa actcaagtcc gtgaaggagc tactcggtat caccatcatg     9540

gagcgatctt cttttgagaa gaaccccatt gacttcctcg aggccaaggg ttacaaagag     9600

gtcaagaagg acctgattat caagctgccc aagtactccc tctttgagct cgagaacggc     9660

cgaaagcgaa tgctggcttc cgctggtgag ctgcagaagg gcaacgagct cgctctgccc     9720

tccaagtacg tcaacttcct ctacctggcc tcccactacg agaagctcaa gggctccccc     9780

gaggacaacg agcagaagca gctgttcgtt gagcagcaca agcactacct cgacgagatc     9840

atcgagcaga tctccgagtt ctccaagcga gtcatcctcg ctgacgccaa ccttgataag     9900

gttctctctg cttacaacaa gcaccgggac aagcccatcc gagagcaggc cgagaatatc     9960

atccacctct tcactctcac caacctcggc gctcctgctg ccttcaagta cttcgacacc    10020

accattgacc gaaagaggta cacctccacc aaggaagtcc tcgacgccac cctgatccac    10080

cagtccatca ccggcctcta cgaaacccga atcgacctct cccagctcgg cggtgactct    10140

cgagccgacc ccaagaagaa gcgaaaagtc taaatatccg aagatcaaga gcgaagcaag    10200

ttgtaagtcc aggacatgtt tcccgcccac gcgagtgatt tataacacct ctcttttttg    10260

acacccgctc gccttgaaat tcatgtcaca taaattatag tcaacgacgt ttgaataact    10320

tgtcttgtag ttcgatgatg atcatatgat tacattaata gtaattactg tatttgatat    10380

atatactaat tacaatagta catattagaa catacaatag ttagtgccgt gaagtggctt    10440

aaaataccgc gagtcgatta cgtaatatta ttacctcttg cccatcgaac gtacaagtac    10500

tcctctgttc tctccttcct ttgctttgtg cacgaagaac tgcggtcagg tgacacaact    10560

ttttccatct cagggtgtgt cgcgtgtgct tcatccaaac tttagttggg gttcgggttc    10620

gcgcgagatg atcacgtgcc ctgatttggt gtcgtccccc gtcgcgctgc gcacgtgatt    10680

tatttatttc cggtggctgc tgtctacgcg gggccttctc tgcccttctg tttcaacctt    10740

cgggcggttc tcgtaaccag cagtagcaat ccatttcgaa actcaaagag ctaaaaacgt    10800

taaacctcag cagtcgctcg acgaatgggc tgcggttggg aagcccacga ggcctatagc    10860

cagagcctcg agttgacagg agcccagacg ccttttccaa cggcaacttt tatataaaat    10920

ggcaatgtat tcatgcaatt gcggccgtgt caggttggag acactggacc acactctcca    10980

ttgcttcctg aggagatgga tcattgctag tgcatctacg cgcagcaatc ccgcaagctc    11040

gacaaccgta gatgggcttt ggtgggccaa tcaattacgc aacccgcacg ttaaattgta    11100

tgaggaagga aggccacggt acaaagtggg tggtcttcac ccagtggttg ttggtggcgt    11160

catgcagacc atggccgcca gtgtgctgga attgaatatt taccgttcgt ataatgtatg    11220

ctatacgaag ttataccggt ctcgtagtgt tcacgttcag ttcacggtga gcttaaaact    11280

atcttcaaga agagatttga gacctgattt atacttgcag caatgtttac ttcttatcgc    11340

gatacacgaa tgtgatacgg atcaaagtaa gcaggactac gataagataa cgaatgcggt    11400

gcagtccatg tcgattaggt atagatacat ttattttgtg ttatgttaca ttttgggggg    11460

atactgtcct acttgtagta cctacttgta gtggcgcgtt aggggcaggg catgctcatg    11520

tagagcgcct gccgctcgcc gtccgaggcg gtgccgtcgt acagggcggt gtccaggccg    11580

cagagggtga accccatccg ccggta                                         11606


<210>  10
<211>  14006
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression plasmid

<400>  10
gcggagccta ggccggccag cgtggcgcgt ggccatattg gccagcagct tactacagct       60

cagccaaagt ggatcatgat ggacggaaat atcggactgg aggccaaaaa ggaggtgctc      120

aaatacgcac gggacaagtc tgcacaggtg gcattcgaac ccacgtctgt ccccaaagcc      180

gctgctcttt ccgagctcaa tctgcccgtt taccccaaca actccattgc cttagccacc      240

cccaacactg cagagctcga ggccatgttc gaggcattcc acgagaaggg ccgatttgac      300

gttgacgact ggtttccagt catagacggt cttgctctcg gagctgactt tagaaacgga      360

gccaccatgt tgtcgcacca acatcgtgga ctcaaagcta ttctcgaaca aggaactctg      420

gcccaggcaa tccacatgct accatacatc ccaactttga tcatcaaggg cggagccaac      480

ggcgtcgtcg tcttccaact cattgatgat atcgagtctg ccattcattc acagcgttct      540

gcttccaata aaacgcctgg cctgttccag aagggcaacg ctgcgagtgg aaacaccaag      600

gtcggcgtct acatgcagta ctttgagccc gaagaagtgg gcagtcagtc gattgtaagc      660

gtgactggag ctggagacac tctgtttgga accctggcca tggagattgt caaggacgag      720

tcctggttga acgatatggg agacaagaag agtgcagttg tttctcgagc catgaacaat      780

gctgtgaaga ctattcagag taaggacgct gtctgcaaga gcatccttta agtgatttgc      840

catgctttct cttcttccac gatgtaaata cttattttac acactactgt gcagtagcaa      900

atacagaaca agagttgtcg cctattgaca gtacagtacg agtagtgtat gtacagtagt      960

tatacaatat ctatgtgaaa ttcgtcggca gctttcggct gatgaactac gagttcttcg     1020

taataaatca tcaacgtaat aagcttggta ccagagacgg gttggcggcg tatttgtgtc     1080

ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc gggcccaacc     1140

ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac acttgccgtt     1200

aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta ctagtttctt     1260

tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa atttttttgc     1320

tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat tacctttcct     1380

cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt tccttctgag     1440

tataagaatc attcgctagc cacaaaaatg ggtgatctcg atgcccgagg aacctctgct     1500

caccccgagc tctctgagcg accttctatt atgccttcta tgtctgatat tcaggaccct     1560

tctggtgatg acaaggctac tccccgagga tctgctgctg gtctgcccca gcttgagctt     1620

gctggacacg cccgacgact tggccacctt gagaacttct ttgctgtcca ggctcgacag     1680

cagatttact cttcttttgc tgttttttgt gagtttgaca ctgcttgttc tctcgctcag     1740

cttgcttctg ctgtgcgaaa cgtttgtctt tctaaccccc ttctccttca cactgttgag     1800

cctaagcacc ctgacatcgc tggattctac cactctgacg agtacctttc ccgaccttgg     1860

cctcagcacg attacatgcg agttcttcga gaggttcacg tcgctgacgt tgttatgaac     1920

ggacagaagg agcacgctca cgttgttcga gatgctgttg acgtttttca ggctcacgga     1980

aaccaggtta cttctgagct ccttgagctt atgactcaga ttgagattcc tcacgcttct     2040

cagactcgac cctcttggcg acttctctgt tttccccacg gagaggctaa ccgatggcga     2100

acctttgctt ttgtttctaa ccactgttct tctgatggtc tttctggtct taacttcttt     2160

cgagatctcc agaaggagct tgctcacggc cccacctctg gtgctcctgg tgcccccgga     2220

gcttccggag ttattttcga ttacgctcag gacgctgcta ccctgcccaa gctgccccct     2280

cccattgatc agaagctcga ttaccgacct tctaagaagg ctcttctcgg ccttctcgct     2340

ggcaagttcg ttcgagagaa gctcggttac gtttctgctg ctcctcccac tacccctacc     2400

tctgaccttg ctcaccctga gggtcaccag tactactgtt accttgttaa cgttcccact     2460

tcttctgttg cccacattaa gactcaggtg cgagagaacg ttcctcacaa gtgtactctc     2520

actccctttc tccaggcttg ttggcttgtt tctctgttca agtacggtcg agttttttct     2580

ggttcttggc ttgagcgata caccgatgtt cttgttgcta tgaacactcg acagcttctc     2640

cccgaggacc ttgagcttca gcgacagtac cgatacggtt ctaacattgg agctgttcga     2700

tacaactacc ctattgctcc ccttgacgtt cgagataacg atcagaagtt ctggtccctt     2760

gttgagtctt accgacttgc cctttctgat gcccgagata agaacgatta cctttacgct     2820

cttggtgctc ttatgctccc tgagatttac gagaagaaga acgttgatgc tgttgttaac     2880

gataccattc ttaaccagcg acgacaggga acccttcttt ctaacgttgg ttacgttcga     2940

gatgagcagc ccactgcttt tgctattaag aaccacgttt tttctcaggg agttggagct     3000

aaccgaaacg cttttgttct taacatttgt gctaccgatc agggtggtct taacatcgct     3060

atttctattg ctaagggaac ccttgcttct cgacaggagg gacaggagct ttgtgatatt     3120

tttaagtcta ctctccttcg attttaaacg cgtctatccg aagatcaaga gcgaagcaag     3180

ttgtaagtcc aggacatgtt tcccgcccac gcgagtgatt tataacacct ctcttttttg     3240

acacccgctc gccttgaaat tcatgtcaca taaattatag tcaacgacgt ttgaataact     3300

tgtcttgtag ttcgatgatg atcatatgat tacattaata gtaattactg tatctgtacc     3360

tgctgtggac cacgcacggc ggaacgtacc gtacaaatat tttcttgctc acatgactct     3420

ctctcggccg cgcacgccgg tggcaaattg ctcttgcatt ggctctgtct ctagacgtcc     3480

aaaccgtcca aagtggcagg gtgacgtgat gcgacgcacg aaggagatgg cccggtggcg     3540

aggaaccgga cacggcgagc cggcgggaaa aaaggcggaa aacgaaaagc gaagggcaca     3600

atctgacggt gcggctgcca ccaacccaag gaggctattt tgggtcgctt tccatttcac     3660

attcgccctc aatggccact ttgcggtggt gaacatggtt tctgaaacaa ccccccagaa     3720

ttagagtata ttgatgtgtt taagattggg ttgctatttg gccattgtgg gggagggtag     3780

cgacgtggag gacattccag ggcgaattga gcctagaaag tggtaccatt ccaaccgtct     3840

cagtcgtccg aattgatcgc tataactatc acctctctca catgtctact tccccaacca     3900

acatccccaa cctcccccac actaaagttc acgccaataa tgtaggcact ctttctgggt     3960

gtgggacagc agagcaatac ggaggggaga ttacacaacg agccacaatt ggggagatgg     4020

tagccatctc actcgacccg tcgacttttg gcaacgctca attacccacc aaatttgggc     4080

tggagttgag gggaccgtgt tccagcgctg taggaccagc aacacacacg gtatcaacag     4140

caaccaacgc ccccgctaat gcacccagta ctgcgcaggt gtgggccagg tgcgttccag     4200

atgcgagttg gcgaacccta agccgacagt gtactttttg ggacgggcag tagcaatcgt     4260

gggcgtagac cccggtgtat ataaaggggt ggagaggacg gattattagc accaacacac     4320

acacttatac tacatgctag ccacaaaaat gctctctttc ttctggcgaa acggtatcga     4380

gactcccgag cccctcaagg ctgacgtttc cggctctatc cctccctggc ttcagggaac     4440

ccttctccga aacggtcctg gtctgttctc cgttggcaac acttcctaca agcactggtt     4500

cgatggtatg gctctcattc actccttcac ctttaaggat ggtgaggttt tttaccgatc     4560

taagtacctg aagtctgaga cttacaagaa gaacatcgct gccgaccgaa tcgttgtgtc     4620

tgagttcgga accatggtgt accccgatcc ctgcaagaac attttctccc gagccttctc     4680

ttacatgatg aacgccattc ctgactttac cgataacaac ctcattaaca tcattaagta     4740

cggtgaggat tactacgcct cctctgaggt caactacatc aaccagattg accccctgac     4800

ccttgagact ctcggacgaa ctaactaccg aaaccacatt gccatcaacc ttgccactgc     4860

tcaccctcac tacgacgagg agggtaacac ttacaacatg ggcactgcta ttatgaacct     4920

cggtcgaccc aagtacgtga ttttcaaggt gcccgccaac acctctgata aggagaacaa     4980

gaagcctgcc ctctctgagg tggagcaggt ttgctccatt cccatccgac cctcccttta     5040

cccttcttac ttccactctt ttggcatgac tgagaactac atcatcttcg ttgagcaggc     5100

cttcaagctg gacatcgtca agctggctac tgcttacttc cgagatatta actggggatc     5160

ttgccttaag ttcgaccagg atgacattaa cgtgttccac ctggtcaaca agaagactgg     5220

taaggctgtg tccgtgaagt actacactga cccctttgtt accttccacc acatcaacgc     5280

ttacgaggac gatggccacg tcgtcttcga tctcattact tacaaggact ctaagctgta     5340

cgatatgttc tacattcaga acatgaagca ggacgtcaag cgatttattg agactaacaa     5400

ggacttcgct cagcccgtgt gccagcgatt tgtccttccc gtcaacgttg ataaggagac     5460

tcctcaggac atcaaccttg tcaagctgca ggacaccact gccactgctg tcctgaagga     5520

ggacggctct gtctactgca cccctgacat catttttaag ggtcttgagc tccctgctat     5580

caactacaag tttaactcta agaagaaccg atacttctac ggcacccgag tggagtggtc     5640

cccttaccct aacaaggtcg ctaaggtgga cgttgttact cgaacccaca agatttggac     5700

tgaggaggag tgttaccctt ctgagcctgt ctttattgcc tcccctgacg ccgttgatga     5760

ggatgacggt gtgattcttt cttctgtggt ttctttcaac ccccagcgac cccctttcct     5820

ggttgtcctc gatgctaagt ccttcaagga gattgctcga gctaccatcg atgcctctat     5880

tcacatggac cttcacggcc ttttcatcca cgacaagtct acctaagttt tttgatcaat     5940

gatccaatgg ctttcacata cccccccacg cctataatta aaacacagag aaatataatc     6000

taacttaata aatattacgg agaatctttc gagtgttcag cagaaatata gccattgtaa     6060

caaaagccgg ctatcgaccg ctttatcgaa gaatatttcc cgccccccag tggccaaacg     6120

atatcggtca gaaggggcag ctctaaacga agaactgcgg tcaggtgaca caactttttc     6180

catctcaggg tgtgtcgcgt gtgcttcatc caaactttag ttggggttcg ggttcgcgcg     6240

agatgatcac gtgccctgat ttggtgtcgt cccccgtcgc gctgcgcacg tgatttattt     6300

atttccggtg gctgctgtct acgcggggcc ttctctgccc ttctgtttca accttcgggc     6360

ggttctcgta accagcagta gcaatccatt tcgaaactca aagagctaaa aacgttaaac     6420

ctcagcagtc gctcgacgaa tgggctgcgg ttgggaagcc cacgaggcct atagccagag     6480

cctcgagttg acaggagccc agacgccttt tccaacggca acttttatat aaaatggcaa     6540

tgtattcatg caattgcggc cgtgtcaggt tggagacact ggaccacact ctccattgct     6600

tcctgaggag atggatcatt gctagtgcat ctacgcgcag caatcccgca agctcgacaa     6660

ccgtagatgg gctttggtgg gccaatcaat tacgcaaccc gcacgttaaa ttgtatgagg     6720

aaggaaggcc acggtacaaa gtgggtggtc ttcacccagt ggttgttggt ggcgtcatgc     6780

agaccatgca ttggggatag cacagggttg gggtgtcttg tggactcaat gggtgaaagg     6840

agatggaaaa gggcggtgaa aagtggtaga atcgaaatcc ctgacgtcaa tttataaagt     6900

aaaatgcgtt tctgccattt tgctcccctc cttctttcgc aatcgcctcc ccaaaagttg     6960

tcgtggcagt acacatgctt gcatacaatg aagctaatcc ggcttgctca gtagttgcta     7020

tatccaggca tggtgtgaaa cccctcaaag tatatatagg agcggtgagc cccagtctgg     7080

ggtcttttct ctccatctca aaactacttt ctcacatgct agccacaaaa atgaccacta     7140

agtacacttc cgttcacgag tctcccaacg gccctggtga cgctcgaccc accgcttccc     7200

agattatcga cgattacaac cttgagggag agctttctgg caagactgtt ctcgtcaccg     7260

gctgttcctc tggtattggt gttgagactg cccgagctat ttaccgaact ggtgccaccc     7320

tttacctcac tgcccgagat gtcgataagg ccaagaccgt tcttcccgac cttgttgaca     7380

cttcccgagt ccactttctc caccttgacc ttaactctct ggagtctgtt cgaggttttg     7440

ctgagaactt caagtctaag tccactcagc ttcacattct catcgagaac gctggcgtga     7500

tggcctgtcc cgagggccga accgtcgatg gttttgagac tcagtttggt atcaaccacc     7560

ttgctcactt tctcctcttt tacctcctca aggataccct tctcaactct tctacccccg     7620

ctttcaactc ccgagttgtc atcctctctt cttgtgctca ccaggctggt tccgttcacc     7680

ttaacaacct gtctcttgag ggtggatacg agccttggaa gtcttacggc cagtccaaga     7740

ctgccaacct ttggactgcc cgagagatcg agaagcgatt tggtgcttcc ggtatccact     7800

cttgggctgt tcaccccggt tccatcgcta ctgagcttca gcgacacgtt tccgacgagc     7860

ttaagcagaa gtgggctgac gataaggagg gtgccaagct gtggaagtcc accgagcagg     7920

gtgccgccac cactgtcctt gctgctgttt cccctgagct tgagggtaag ggcggtcttt     7980

accttgagga tacccaggtt gccaagcccc ctgcccgagg aatgtttggt gttgctgact     8040

gggcttacga tgaggatggc ccctctaagc tctgggccaa gtctcttgag ctccttaagc     8100

tccagtaaag gttagactat ggatatgtaa tttaactgtg tatatagaga gcgtgcaagt     8160

atggagcgct tgttcagctt gtatgatggt cagacgacct gtctgatcga gtatgtatga     8220

tactgcacaa cctgtgtatc cgcatgatct gtccaatggg gcatgttgtt gtgtttctcg     8280

atacggagat gctgggtaca agtagctaat acgattgaac tacttatact tatatgaggc     8340

ttgaagaaag ctgacttgtg tatgacttat tctcaactac atccccagtc acaataccac     8400

cactgcacta ccactacaca ctagtggtgt gttctgtgga gcattctcac ttttggtaaa     8460

cgacattgct tcaagtgcag cggaatcaaa aagtataaag tgggcagcga gtatacctgt     8520

acagactgta ggcgataact caatccaatt accccccaca acatgactgg ccaaactgat     8580

ctcaagactt tattgaaatc agcaacaccg attctcaatg aaggcacata cttcttctgc     8640

aacattcact tgacgcctaa agttggtgag aaatggaccg acaagacata ttctgctatc     8700

cacggactgt tgcctgtgtc ggtggctaca atacgtgagt cagaagggct gacggtggtg     8760

gttcccaagg aaaaggtcga cgagtatctg tctgactcgt cattgccgcc tttggagtac     8820

gactccaact atgagtgtgc ttggatcact ttgacgatac attcttcgtt ggaggctgtg     8880

ggtctgacag ctgcgttttc ggcgcggttg gccgacaaca atatcagctg caacgtcatt     8940

gctggctttc atcatgatca catttttgtc ggcaaaggcg acgcccagag agccattgac     9000

gttctttcta atttggaccg atagccgtat agtccagtct atctataagt tcaactaact     9060

cgtaactatt accataacat atacttcact gccccagata aggttccgat aaaaagttct     9120

gcagactaaa tttatttcag tctcctcttc accaccaaaa tgccctccta cgaagctcga     9180

gctaacgtcc acaagtccgc ctttgccgct cgagtgctca agctcgtggc agccaagaaa     9240

accaacctgt gtgcttctct ggatgttacc accaccaagg agctcattga gcttgccgat     9300

aaggtcggac cttatgtgtg catgatcaag acccatatcg acatcattga cgacttcacc     9360

tacgccggca ctgtgctccc cctcaaggaa cttgctctta agcacggttt cttcctgttc     9420

gaggacagaa agttcgcaga tattggcaac actgtcaagc accagtacaa gaacggtgtc     9480

taccgaatcg ccgagtggtc cgatatcacc aacgcccacg gtgtacccgg aaccggaatc     9540

attgctggcc tgcgagctgg tgccgaggaa actgtctctg aacagaagaa ggaggacgtc     9600

tctgactacg agaactccca gtacaaggag ttcctggtcc cctctcccaa cgagaagctg     9660

gccagaggtc tgctcatgct ggccgagctg tcttgcaagg gctctctggc cactggcgag     9720

tactccaagc agaccattga gcttgcccga tccgaccccg agtttgtggt tggcttcatt     9780

gcccagaacc gacctaaggg cgactctgag gactggctta ttctgacccc cggggtgggt     9840

cttgacgaca agggagacgc tctcggacag cagtaccgaa ctgttgagga tgtcatgtct     9900

accggaacgg atatcataat tgtcggccga ggtctgtacg gccagaaccg agatcctatt     9960

gaggaggcca agcgatacca gaaggctggc tgggaggctt accagaagat taactgttag    10020

aggttagact atggatatgt aatttaactg tgtatataga gagcgtgcaa gtatggagcg    10080

cttgttcagc ttgtatgatg gtcagacgac ctgtctgatc gagtatgtat gatactgcac    10140

aacctgtgta tccgcatgat ctgtccaatg gggcatgttg ttgtgtttct cgatacggag    10200

atgctgggta caagtagcta atacgattga actacttata cttatatgag gcttgaagaa    10260

agctgacttg tgtatgactt attctcaact acatccccag tcacaatacc accactgcac    10320

taccactaca cctcgagcat gcacactatt atcacattac tacatccaaa cccctacagg    10380

gggaggagct ctccaatcaa atatgtacat taactatctc tcgtaaatca ttgttataag    10440

accgtccgtg actgtctaaa tcggttcatt cgttgtaaca aatcagtgta acaactcgtg    10500

gtacgcccgg tttgcttcgc tcacgctccc ctagattttc cgtctaggac acaacaagcc    10560

ctggtggatg acgttcaacg ccttcagcgc gtctttttcc tcaataacac acgagatgtt    10620

aatttcgttg gctccctgag aaatcatctc aatgttaata cctgcctggg ccagagtaga    10680

gaagaacatg cctgcacatc cgaccatggc cttcattctc gttccgatca gcgacacaat    10740

ggtcatgcct cgcttcacat ctactgtgcc gtactttcgc agctcctcca cagcctgctt    10800

gaggttggag tcgggagcat ggaaggccat cgacacatgg acctcagaag tggagatgag    10860

atcgacgaca agtttctgct ggtcgagagt agcaaagatc ttgttcaaga agccatgact    10920

cttggttcgc ttattcgaat gaacgttgag aacggtgata ttcgatttgg tagtgacagc    10980

tgtaggcttc ttctcgtcat ctacaggagc agacacctgg ggagtgaatg agccagagga    11040

cagagaagtt agcgagtcgg tggaagaagc cagatcctgg acattgtttt tcgttttcag    11100

gttgcccgag ccgtcagcag atgggtagat gatagttcct cctcccaggg ggttttccac    11160

gtttttgatt cggataggaa tatgggcctt aatgacctgc tccatggtga aggggtggat    11220

gacttcagat ccgtagtagg tcaactcagc ggcctcctcg ggggtaatga tgggcagcag    11280

acgggcagtg gacacctttc tgggatcggc agtgaagaca ccatcgactt ctttccagat    11340

ctgaagctcc ttggcgtcca gacccacagc caccagagca gcacacagat cggtgtatcc    11400

tcgtccgatc tggcttagga ggcctccctt gacggggcca aagaaaccgg tcaacacggg    11460

caccatgttt cccttgacag gagcagagtc cgtgttagga gagccgggaa gagtgatgat    11520

ctcgcccaga acacgtccga ggtccgaata gaacttggga tcttcggcat tggtggtggt    11580

aactgcgtga gacaggtcaa agtaccgtgc gttaacacca gcgtcccgca taactgcagt    11640

catgtacatg caactgagct tctccccaat ggccatgatg gagtcgagtg ttcgggggga    11700

gatctcagaa atgatttcag cagcagccag gattcgcaac agctggtcgc actcgccgtt    11760

gatgtcggca ttgaggtttt cgagcagttc ggggttcttg acgtcacgct tagcagctgc    11820

aagatggtct tctcggatag actcaatgat ggggttgtag gcgtctgatc cgagaagagc    11880

agagtcagca gcagcaataa gacgggtggt ggttccctcg gccttggtgg cctctagatg    11940

gcctccttgg ccccattcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt    12000

ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg    12060

ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg    12120

gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag    12180

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga    12240

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct    12300

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc    12360

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg    12420

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc    12480

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca    12540

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag    12600

ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct    12660

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc    12720

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga    12780

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca    12840

cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat    12900

taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac    12960

caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt    13020

gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt    13080

gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag    13140

ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct    13200

attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt    13260

gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc    13320

tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt    13380

agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg    13440

gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg    13500

actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct    13560

tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc    13620

attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt    13680

tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt    13740

tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg    13800

aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat    13860

tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg    13920

cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta    13980

acctataaaa ataggcgtat cacgag                                         14006


<210>  11
<211>  13802
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression plasmid

<400>  11
ggttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta       60

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag      120

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg      180

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg      240

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg      300

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga      360

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc      420

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt      480

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact      540

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg      600

cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt      660

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt      720

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct      780

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg      840

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt      900

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt      960

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     1020

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     1080

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     1140

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     1200

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca     1260

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     1320

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     1380

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     1440

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     1500

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca     1560

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     1620

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     1680

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     1740

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     1800

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     1860

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     1920

aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg     1980

cgtatcacga ggccctttcg tctggcctag gaagcgactt ccaatcgctt tgcatatcca     2040

gtaccacacc cacaggcgtt tgtgctactc tactgatagc aatagatgcg tcataattgg     2100

ttggcccgct gagcctccac aggatactat tgcacatacc ctggtcatgt gcagatcagc     2160

tcatttgtgg agactctgga gtaacttaga cgacgcctgg ttcaattgcc gcaatgtgcg     2220

cccacgcaga taatgtattg aggggtggag cgcctcttgg ggacttgctg tacttgtacg     2280

ggatattaaa cgcactcagc aagaccatga cgtaaaacac acctactgta cgatacgtac     2340

tgtaggtatt gtactcgtac ccggtactac aaatagtacg atactatacg gagtgtattt     2400

gtaccttgat atacgactgg cggagtgaag agaaggagtt gaacaagacc agatggggat     2460

atcagcccca gtgctttgta ttacaagtac gagtacttaa tagatactgt aaggctattg     2520

atacggatgg cagtaagtca ttgagtaagc aattgtggcc cagcatctcc cctacgtact     2580

tgtaccatac cccatggaga caccaatggt ctttcacgca cactgtcgtg tgctgtatcg     2640

cagaatcggg tgtccaacca aatgccgtta cccccacgtc acagccgata gacagataca     2700

ccatcaatac cagcaggttg tatcatgcgg ttggctgaag gtaagctgat tggtctaaaa     2760

actgtagctg tcctaattca acgagcgcta tttggggcca accacctcgg ccaagcggcc     2820

tttaatctgc gtgccccaga ggcgtctaat gaggctctgg ccgccactgt aggagtgttt     2880

ctctgtgcgc acacgcagtt ttgagtttgg gcgactttcc ctttttccca attgcgtaca     2940

cacacagctc cgagctaagc gctgtccttg aaccttctcc ctcttttccc tctttttctc     3000

ttccccttcc cctcctccac attaaggcca aatcctgaat tgcaccaact agtacaacga     3060

caacaatgga caagaagtac tccatcggtt tggacattgg tactaactct gtcggctggg     3120

ccgtcatcac cgacgagtac aaggttccct ccaagaagtt caaggtcctt ggcaacaccg     3180

accgacactc tatcaagaag aacctgatcg gtgctctgct gttcgactct ggcgagactg     3240

ccgaggccac ccgactgaag cgaaccgctc gacgccgata cacccgacga aagaaccgaa     3300

tctgttacct ccaggagatc ttcagcaacg agatggctaa ggtcgacgac tccttcttcc     3360

accgactcga ggagtctttc ctggtcgaag aggataagaa gcacgagcga caccccatct     3420

tcggcaacat tgttgatgag gttgcctacc atgagaagta ccccaccatc taccacctcc     3480

gaaagaagct cgtcgactcc actgacaagg ctgacctccg actcatctac cttgctctcg     3540

cccacatgat caagttccga ggtcacttcc tcattgaggg tgatctcaac cccgacaact     3600

ccgacgttga caagctgttc atccagctcg tccagaccta caaccagctc tttgaggaga     3660

accctatcaa cgcttctggt gttgacgcca aggccattct ctccgcccga ctctctaagt     3720

cccgacgact cgagaacctc attgcccagc tgcccggcga gaagaagaac ggcctcttcg     3780

gtaacctgat tgctctctct cttggtctga cccccaactt caagtccaac tttgacctcg     3840

ccgaggacgc caagctccag ctgtccaagg acacctacga tgacgatctg gacaacctcc     3900

tggcccagat cggtgaccag tacgccgatc tcttccttgc cgccaagaac ctctccgacg     3960

ccatcctgct ctccgacatc ctccgagtca acaccgagat taccaaggct cctctgtctg     4020

cctctatgat caagcgatac gacgagcacc accaggatct cactcttctc aaggctctcg     4080

tccgacagca gctccccgag aagtacaagg agattttctt tgaccagtcc aagaacggtt     4140

acgctggcta cattgacggt ggtgcttccc aggaagagtt ttacaagttc atcaagccta     4200

ttctggagaa gatggacggt accgaggagc tgctcgtcaa gctcaaccga gaggacctcc     4260

ttcgaaagca gcgaaccttc gataacggct ccatccccca ccagatccac ctgggtgagc     4320

tccacgccat tctccgaaga caagaggact tctacccctt cctaaaggat aaccgagaga     4380

agatcgagaa gattctcacc ttccgaatcc cctactacgt cggtcccctc gctcgaggta     4440

actcccgatt tgcttggatg acccgaaagt ccgaggagac tatcaccccc tggaactttg     4500

aagaggtagt cgacaagggt gcctccgccc agtctttcat tgagcggatg accaacttcg     4560

ataagaacct ccccaacgag aaggtccttc ccaagcactc tctcctctac gagtacttca     4620

ccgtctacaa cgagctgacc aaggtcaagt acgttaccga gggcatgcga aagcccgctt     4680

tcctctctgg tgagcagaag aaggccattg tcgacctcct gttcaagact aaccgaaaag     4740

tcaccgtcaa gcagctcaag gaagactact tcaagaagat tgagtgcttc gactccgtcg     4800

agatttccgg tgtcgaggac cgattcaacg cctccctcgg cacctaccac gatcttctga     4860

agatcatcaa ggacaaggac tttcttgata acgaggagaa cgaggacatt ctcgaggaca     4920

tcgtcctcac cctcaccctt ttcgaggatc gagagatgat cgaggagcga ctcaagacct     4980

acgcccatct cttcgacgac aaggtcatga agcaactcaa gcgacgacga tacactggct     5040

ggggccgact ttcccgaaag ctcatcaacg gcatccgaga caagcagtct ggcaagacca     5100

tcctggactt cctgaagtcc gacggtttcg ccaaccgaaa cttcatgcag ctcatccacg     5160

acgactctct taccttcaaa gaggatatcc agaaggccca ggtttctggc cagggcgact     5220

ccctccacga gcacattgcc aacctcgccg gatcccccgc catcaaaaag ggtatcctcc     5280

agaccgtcaa ggttgtcgac gaactcgtga aggtcatggg ccgacacaag cccgagaaca     5340

tcgttatcga gatggcccga gagaaccaga ccacccagaa gggtcagaag aactcccgag     5400

agcgaatgaa gcgaatcgaa gagggtatca aggagctcgg ttcccagatt ctcaaggagc     5460

accccgtcga gaacacccag ctccagaacg agaaactcta cctgtactac ctccagaatg     5520

gccgagacat gtacgttgac caggagctcg acatcaaccg actctccgac tacgacgtcg     5580

accacattgt tcctcagtcc ttcctcaagg acgactccat cgacaacaag gttctgaccc     5640

gatctgacaa gaaccgaggt aagtccgaca acgttccctc cgaagaggtc gttaagaaga     5700

tgaagaacta ctggcgacag cttctcaacg ccaaactgat cacccagcga aagtttgaca     5760

acctcaccaa ggccgagcga ggtggtctgt ccgagctgga caaggccggc ttcattaagc     5820

gacagctggt cgagactcga cagatcacca agcacgtcgc ccagatcctc gactcccgaa     5880

tgaacaccaa gtacgacgag aacgacaagc tcatccggga ggtcaaggtc atcaccctga     5940

agtctaagct tgtctccgac ttccgaaagg acttccagtt ctacaaggtc cgagagatca     6000

acaactacca ccacgcccac gacgcctacc tcaacgccgt tgttggtacc gccctcatca     6060

agaagtatcc caagctcgag tccgagttcg tttacggcga ctacaaggtt tacgatgtcc     6120

gaaagatgat tgccaagtcc gagcaggaga tcggtaaggc caccgccaag tactttttct     6180

actccaacat catgaatttc ttcaagaccg agatcactct cgccaacggt gagattcgaa     6240

agcgacccct gattgagact aatggtgaga ctggtgagat cgtctgggat aagggccgag     6300

acttcgccac cgtccgaaag gtcctgtcca tgccccaggt caacattgtc aagaagaccg     6360

aggtccagac cggtggcttc tccaaggagt ccattctccc caagcgaaac tccgacaaac     6420

tcatcgcccg taagaaggac tgggatccga agaagtacgg tggtttcgat tctcccaccg     6480

ttgcctactc cgtcctcgtt gttgctaaag tcgagaaggg taagtctaag aaactcaagt     6540

ccgtgaagga gctactcggt atcaccatca tggagcgatc ttcttttgag aagaacccca     6600

ttgacttcct cgaggccaag ggttacaaag aggtcaagaa ggacctgatt atcaagctgc     6660

ccaagtactc cctctttgag ctcgagaacg gccgaaagcg aatgctggct tccgctggtg     6720

agctgcagaa gggcaacgag ctcgctctgc cctccaagta cgtcaacttc ctctacctgg     6780

cctcccacta cgagaagctc aagggctccc ccgaggacaa cgagcagaag cagctgttcg     6840

ttgagcagca caagcactac ctcgacgaga tcatcgagca gatctccgag ttctccaagc     6900

gagtcatcct cgctgacgcc aaccttgata aggttctctc tgcttacaac aagcaccggg     6960

acaagcccat ccgagagcag gccgagaata tcatccacct cttcactctc accaacctcg     7020

gcgctcctgc tgccttcaag tacttcgaca ccaccattga ccgaaagagg tacacctcca     7080

ccaaggaagt cctcgacgcc accctgatcc accagtccat caccggcctc tacgaaaccc     7140

gaatcgacct ctcccagctc ggcggtgact ctcgagccga ccccaagaag aagcgaaaag     7200

tctaaatatc cgaagatcaa gagcgaagca agttgtaagt ccaggacatg tttcccgccc     7260

acgcgagtga tttataacac ctctcttttt tgacacccgc tcgccttgaa attcatgtca     7320

cataaattat agtcaacgac gtttgaataa cttgtcttgt agttcgatga tgatcatatg     7380

attacattaa tagtaattac tgtatttgat atatatacta attacaatag tacatattag     7440

aacatacaat agttagtgcc gtgaagtggc ttaaaatacc gcgagtcgat tacgtaatat     7500

tattacctct tgcccatcga acgtacaagt actcctctgt tctctccttc ctttgctttg     7560

tgcacgaaga actgcggtca ggtgacacaa ctttttccat ctcagggtgt gtcgcgtgtg     7620

cttcatccaa actttagttg gggttcgggt tcgcgcgaga tgatcacgtg ccctgatttg     7680

gtgtcgtccc ccgtcgcgct gcgcacgtga tttatttatt tccggtggct gctgtctacg     7740

cggggccttc tctgcccttc tgtttcaacc ttcgggcggt tctcgtaacc agcagtagca     7800

atccatttcg aaactcaaag agctaaaaac gttaaacctc agcagtcgct cgacgaatgg     7860

gctgcggttg ggaagcccac gaggcctata gccagagcct cgagttgaca ggagcccaga     7920

cgccttttcc aacggcaact tttatataaa atggcaatgt attcatgcaa ttgcggccgt     7980

gtcaggttgg agacactgga ccacactctc cattgcttcc tgaggagatg gatcattgct     8040

agtgcatcta cgcgcagcaa tcccgcaagc tcgacaaccg tagatgggct ttggtgggcc     8100

aatcaattac gcaacccgca cgttaaattg tatgaggaag gaaggccacg gtacaaagtg     8160

ggtggtcttc acccagtggt tgttggtggc gtcatgcaga ccatgcattg gggatagcac     8220

agggttgggg tgtcttgtgg actcaatggg tgaaaggaga tggaaaaggg cggtgaaaag     8280

tggtagaatc gaaatccctg acgtcaattt ataaagtaaa atgcgtttct gccattttgc     8340

tcccctcctt ctttcgcaat cgcctcccca aaagttgtcg tggcagtaca catgcttgca     8400

tacaatgaag ctaatccggc ttgctcagta gttgctatat ccaggcatgg tgtgaaaccc     8460

ctcaaagtat atataggagc ggtgagcccc agtctggggt cttttctctc catctcaaaa     8520

ctactttctc acaatggatt tgctgatgag tccgtgagga cgaaacgagt aagctcgtct     8580

ggaggcatga tcaacagcgg ttttagagct agaaatagca agttaaaata aggctagtcc     8640

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg gccggcatgg tcccagcctc     8700

ctcgctggcg ccggctgggc aacatgcttc ggcatggcga atgggactaa acttcgagct     8760

aatccagtag cttacgttac ccaggggcag gtcaactggc tagccacgag tctgtcccag     8820

gtcgcaattt agtgtaataa acaatatata tattgagtct aaagggaatt gtagctattg     8880

tgattgtgtg attttcgtct tgctggttct tattgtgtcc cattcgtttc atcctgatga     8940

ggacccctgg aaccggtgtt ttcttagtct ctgcaatcgc tagtcttgtt gctatgacag     9000

ttgcgtcgac actattcagg tcatctatcg gttattctga tattataata cctccggatc     9060

gatgtacctg atttatactt gcagcaatgt ttacttctta tcgttggacc ccgtcttcaa     9120

ttacacttcc caactgggaa cacccctctt tatcgaccca ttttaggtaa tttaccctag     9180

cccattgtct ccataaggaa tattacccta acccacagtc cagggtgccc aggtccttct     9240

ttggccaaat tttaacttcg gtcctatggc acagcggtag cgcgtgagat tgcaaatctt     9300

aaggtcccga gttcgaatct cggtgggacc tagttgaaaa atacctctaa tgcgccgatg     9360

gtttagtggt aaaatccatc gttgccatcg atgggccccc ggttcgattc cgggtcggcg     9420

caggttgacg tacagcaggc tgaacgagga tgttttagag ctagaaatag caagttaaaa     9480

taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttttcga     9540

tcggccccag ttgcaaaagt tgacacaact ctagatctgc ttccaaatat agaatcataa     9600

caagggttag ggtgtgatta tataatattg gtcttaattg atgtgctagg gctttaaaag     9660

ttggttaaaa taacgctcta atgccttttt aatatattgt ctttttcaaa atctcaaatc     9720

ggacacttct tcgtgtatga gactccattt tttggctccg tcacgtgata tgtattatca     9780

gctatagtgg tgtaaacaaa gttttttact agctgtaatg gcattttgtc ggagtggtaa     9840

atcgccttct tgttgtgcgt tcgagttctg gactctgcac tgggctactt tgaaaaatac     9900

ctctaatgcg ccgatggttt agtggtaaaa tccatcgttg ccatcgatgg gcccccggtt     9960

cgattccggg tcggcgcagg ttgacgttca ctcctcagcc tcccaaggtt ttagagctag    10020

aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg    10080

tgcttttttt tttcgcgata cacgaatgtg atacggatca aagtaagcag gactacgata    10140

agataacgaa tgcggtgcag tccatgtcga ttaggtatag atacatttat tttgtgttat    10200

gttacatttt ggggggatac tgtcctactt gtagtaccta cttgtagtgg cgcgtctatt    10260

cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac    10320

agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg    10380

ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc    10440

cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc    10500

gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac    10560

aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca    10620

tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg    10680

agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca    10740

gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt    10800

gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga    10860

ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg    10920

catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt    10980

gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc    11040

caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac    11100

gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta    11160

catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc    11220

tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca    11280

tatgggtacc tgagaacatt tttgtgtcta ggtgtttgtg tttggactgc gatcagtgaa    11340

gaaaagaaga ggaaaaattg tgcaagaaat tttgctttca agacttggct gatgcagcag    11400

ggtaactctg ggacacagac ctatgtttgt ggttaaactc aatgcacgtg gtacgtgcgt    11460

ggagcgctta cccatccaag ggtgtggaca tggaaccgac ggtccgtgga gttgtgtaat    11520

gtcattttgg cgactcttga agcaaggcta taaaaaaatt gtgtggcttg agtcttatcg    11580

agctcggtca ctacaagagt taatcttcct gtctcaggca gacaggtcag gcagggttac    11640

ttttgggtgt gctgtaactc actgtatggc cgttagtgcg catagacgtt gtacatactg    11700

gaccgaattg tagcgtgctc aatagggcca ataaagctat tgtagggatc cgaattttca    11760

gaacctaatt tatctgttac ccggcctgtg gctcgcacag cttaaaaatg gtcaaacttt    11820

ccccttcttg tctttttttc ctcacattca tcaggttctt gtcttgatct ttcaagtgag    11880

tattaattac cgaccttggt tcttcattgg gagagcattg gaagccgtgg tgcagcaacc    11940

acaaaacggt tcttcccctt cgataccttc ttgcctgcct ttcaatacaa gtcggctcga    12000

ttagcggtgg tcgcccccgc cagcggagaa catggaacta acccagaatg agagctaagt    12060

ggagaaagaa gagagtcaga cgactcaagc gaaagcgccg caaggtccga gctcgatcca    12120

aataagcggt ttttaacgga gatttaacac taaatcgaag aacttttccc gtttcatttg    12180

cgaatgagct cgttaacaaa atcccccagt ttttttatcc agctgtaagg attgacatta    12240

gtaatgaatt attgtttggt atatttaaat ctgtagttcc tttctgtccg tgtcggcaac    12300

tgtcgtactc gtgatttact tgtattgacg aatacttact gtagcgcact ctgctgctac    12360

tggtcgtaag gatgtgctat ttcggtgtat ggtgggtttt ttgggggtcg gaaccgaaga    12420

ctgttacacg ggcacggctc gttgtgtaca cgcacagagc tcttgcgagt catgttgtag    12480

ctagctcgtc gtgttcagga actgttcgat ggttcggaga gagtcgccgc ccagaacata    12540

cgcgcaccga tgtcagcaga cagccttatt acaagtatat tcaagcaagt atatccgtag    12600

ggtgcgggtg atttggatct aaggttcgta ctcaacactc acgagcagct tgcctatgtt    12660

acatcctttt atcagacata acataattgg agtttactta cacacggggt gtacctgtat    12720

gagcaccacc tacaattgta gcactggtac ttgtacaaag aatttattcg tacgaatcac    12780

agggacggcc gccctcaccg aaccagcgaa tacctcagcg gtcccctgca gtgactcaac    12840

aaagcgatat gaacatcttg cgatggtatc ctgctgatag tttttactgt acaaacacct    12900

gtgtagctcc ttctagcatt tttaagttat tcacacctca aggggaggga taaattaaat    12960

aaattccaaa agcgaagatc gagaaactaa attaaaattc caaaaacgaa gttggaacac    13020

aaccccccga aaaaaaacaa caaacaaaaa acccaacaaa ataaacaaaa acaaaataaa    13080

tatataacta ccagtatctg actaaaagtt caaatactcg tacttacaac aaatagaaat    13140

gagccggcca aaattctgca gaaaaaaatt tcaaacaagt actggtataa ttaaattaaa    13200

aaacacatca aagtatcata acgttagtta ttttatttta tttaataaaa gaaaacaaca    13260

agatgggctc aaaactttca acttatacga tacataccaa ataacaattt agtatttatc    13320

taagtgcttt tcgtagataa tggaatacaa atggatatcc agagtataca catggatagt    13380

atacactgac acgacaattc tgtatctctt tatgttaact actgtgaggc attaaataga    13440

gcttgatata taaaatgtta catttcacag tctgaacttt tgcagattac ctaatttggt    13500

aagatattaa ttatgaactg aaagttgatg gcatccctaa atttgatgaa agatgaaatt    13560

gtaaatgagg tggtaaaaga gctacagtcg ttttgttttg agataccatc atctctaacg    13620

aaatatctat taaaaatctc agtgtgatca tgagtcattg ccatcctgga aaatgtcatc    13680

atggctgata tttctaactg tttacttgag ataaatatat atttacaaga acttcccttg    13740

aaattaattt agatataaaa tgtttgcggg caagttacta cgaggaataa attatatcta    13800

ga                                                                   13802


<210>  12
<211>  13246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression plasmid

<400>  12
ggttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta       60

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag      120

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg      180

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg      240

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg      300

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga      360

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc      420

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt      480

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact      540

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg      600

cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt      660

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt      720

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct      780

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg      840

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt      900

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt      960

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     1020

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     1080

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     1140

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     1200

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca     1260

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     1320

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     1380

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     1440

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     1500

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca     1560

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     1620

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     1680

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     1740

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     1800

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     1860

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     1920

aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg     1980

cgtatcacga ggccctttcg tctggcctag gaagcgactt ccaatcgctt tgcatatcca     2040

gtaccacacc cacaggcgtt tgtgctactc tactgatagc aatagatgcg tcataattgg     2100

ttggcccgct gagcctccac aggatactat tgcacatacc ctggtcatgt gcagatcagc     2160

tcatttgtgg agactctgga gtaacttaga cgacgcctgg ttcaattgcc gcaatgtgcg     2220

cccacgcaga taatgtattg aggggtggag cgcctcttgg ggacttgctg tacttgtacg     2280

ggatattaaa cgcactcagc aagaccatga cgtaaaacac acctactgta cgatacgtac     2340

tgtaggtatt gtactcgtac ccggtactac aaatagtacg atactatacg gagtgtattt     2400

gtaccttgat atacgactgg cggagtgaag agaaggagtt gaacaagacc agatggggat     2460

atcagcccca gtgctttgta ttacaagtac gagtacttaa tagatactgt aaggctattg     2520

atacggatgg cagtaagtca ttgagtaagc aattgtggcc cagcatctcc cctacgtact     2580

tgtaccatac cccatggaga caccaatggt ctttcacgca cactgtcgtg tgctgtatcg     2640

cagaatcggg tgtccaacca aatgccgtta cccccacgtc acagccgata gacagataca     2700

ccatcaatac cagcaggttg tatcatgcgg ttggctgaag gtaagctgat tggtctaaaa     2760

actgtagctg tcctaattca acgagcgcta tttggggcca accacctcgg ccaagcggcc     2820

tttaatctgc gtgccccaga ggcgtctaat gaggctctgg ccgccactgt aggagtgttt     2880

ctctgtgcgc acacgcagtt ttgagtttgg gcgactttcc ctttttccca attgcgtaca     2940

cacacagctc cgagctaagc gctgtccttg aaccttctcc ctcttttccc tctttttctc     3000

ttccccttcc cctcctccac attaaggcca aatcctgaat tgcaccaact agtacaacga     3060

caacaatgga caagaagtac tccatcggtt tggacattgg tactaactct gtcggctggg     3120

ccgtcatcac cgacgagtac aaggttccct ccaagaagtt caaggtcctt ggcaacaccg     3180

accgacactc tatcaagaag aacctgatcg gtgctctgct gttcgactct ggcgagactg     3240

ccgaggccac ccgactgaag cgaaccgctc gacgccgata cacccgacga aagaaccgaa     3300

tctgttacct ccaggagatc ttcagcaacg agatggctaa ggtcgacgac tccttcttcc     3360

accgactcga ggagtctttc ctggtcgaag aggataagaa gcacgagcga caccccatct     3420

tcggcaacat tgttgatgag gttgcctacc atgagaagta ccccaccatc taccacctcc     3480

gaaagaagct cgtcgactcc actgacaagg ctgacctccg actcatctac cttgctctcg     3540

cccacatgat caagttccga ggtcacttcc tcattgaggg tgatctcaac cccgacaact     3600

ccgacgttga caagctgttc atccagctcg tccagaccta caaccagctc tttgaggaga     3660

accctatcaa cgcttctggt gttgacgcca aggccattct ctccgcccga ctctctaagt     3720

cccgacgact cgagaacctc attgcccagc tgcccggcga gaagaagaac ggcctcttcg     3780

gtaacctgat tgctctctct cttggtctga cccccaactt caagtccaac tttgacctcg     3840

ccgaggacgc caagctccag ctgtccaagg acacctacga tgacgatctg gacaacctcc     3900

tggcccagat cggtgaccag tacgccgatc tcttccttgc cgccaagaac ctctccgacg     3960

ccatcctgct ctccgacatc ctccgagtca acaccgagat taccaaggct cctctgtctg     4020

cctctatgat caagcgatac gacgagcacc accaggatct cactcttctc aaggctctcg     4080

tccgacagca gctccccgag aagtacaagg agattttctt tgaccagtcc aagaacggtt     4140

acgctggcta cattgacggt ggtgcttccc aggaagagtt ttacaagttc atcaagccta     4200

ttctggagaa gatggacggt accgaggagc tgctcgtcaa gctcaaccga gaggacctcc     4260

ttcgaaagca gcgaaccttc gataacggct ccatccccca ccagatccac ctgggtgagc     4320

tccacgccat tctccgaaga caagaggact tctacccctt cctaaaggat aaccgagaga     4380

agatcgagaa gattctcacc ttccgaatcc cctactacgt cggtcccctc gctcgaggta     4440

actcccgatt tgcttggatg acccgaaagt ccgaggagac tatcaccccc tggaactttg     4500

aagaggtagt cgacaagggt gcctccgccc agtctttcat tgagcggatg accaacttcg     4560

ataagaacct ccccaacgag aaggtccttc ccaagcactc tctcctctac gagtacttca     4620

ccgtctacaa cgagctgacc aaggtcaagt acgttaccga gggcatgcga aagcccgctt     4680

tcctctctgg tgagcagaag aaggccattg tcgacctcct gttcaagact aaccgaaaag     4740

tcaccgtcaa gcagctcaag gaagactact tcaagaagat tgagtgcttc gactccgtcg     4800

agatttccgg tgtcgaggac cgattcaacg cctccctcgg cacctaccac gatcttctga     4860

agatcatcaa ggacaaggac tttcttgata acgaggagaa cgaggacatt ctcgaggaca     4920

tcgtcctcac cctcaccctt ttcgaggatc gagagatgat cgaggagcga ctcaagacct     4980

acgcccatct cttcgacgac aaggtcatga agcaactcaa gcgacgacga tacactggct     5040

ggggccgact ttcccgaaag ctcatcaacg gcatccgaga caagcagtct ggcaagacca     5100

tcctggactt cctgaagtcc gacggtttcg ccaaccgaaa cttcatgcag ctcatccacg     5160

acgactctct taccttcaaa gaggatatcc agaaggccca ggtttctggc cagggcgact     5220

ccctccacga gcacattgcc aacctcgccg gatcccccgc catcaaaaag ggtatcctcc     5280

agaccgtcaa ggttgtcgac gaactcgtga aggtcatggg ccgacacaag cccgagaaca     5340

tcgttatcga gatggcccga gagaaccaga ccacccagaa gggtcagaag aactcccgag     5400

agcgaatgaa gcgaatcgaa gagggtatca aggagctcgg ttcccagatt ctcaaggagc     5460

accccgtcga gaacacccag ctccagaacg agaaactcta cctgtactac ctccagaatg     5520

gccgagacat gtacgttgac caggagctcg acatcaaccg actctccgac tacgacgtcg     5580

accacattgt tcctcagtcc ttcctcaagg acgactccat cgacaacaag gttctgaccc     5640

gatctgacaa gaaccgaggt aagtccgaca acgttccctc cgaagaggtc gttaagaaga     5700

tgaagaacta ctggcgacag cttctcaacg ccaaactgat cacccagcga aagtttgaca     5760

acctcaccaa ggccgagcga ggtggtctgt ccgagctgga caaggccggc ttcattaagc     5820

gacagctggt cgagactcga cagatcacca agcacgtcgc ccagatcctc gactcccgaa     5880

tgaacaccaa gtacgacgag aacgacaagc tcatccggga ggtcaaggtc atcaccctga     5940

agtctaagct tgtctccgac ttccgaaagg acttccagtt ctacaaggtc cgagagatca     6000

acaactacca ccacgcccac gacgcctacc tcaacgccgt tgttggtacc gccctcatca     6060

agaagtatcc caagctcgag tccgagttcg tttacggcga ctacaaggtt tacgatgtcc     6120

gaaagatgat tgccaagtcc gagcaggaga tcggtaaggc caccgccaag tactttttct     6180

actccaacat catgaatttc ttcaagaccg agatcactct cgccaacggt gagattcgaa     6240

agcgacccct gattgagact aatggtgaga ctggtgagat cgtctgggat aagggccgag     6300

acttcgccac cgtccgaaag gtcctgtcca tgccccaggt caacattgtc aagaagaccg     6360

aggtccagac cggtggcttc tccaaggagt ccattctccc caagcgaaac tccgacaaac     6420

tcatcgcccg taagaaggac tgggatccga agaagtacgg tggtttcgat tctcccaccg     6480

ttgcctactc cgtcctcgtt gttgctaaag tcgagaaggg taagtctaag aaactcaagt     6540

ccgtgaagga gctactcggt atcaccatca tggagcgatc ttcttttgag aagaacccca     6600

ttgacttcct cgaggccaag ggttacaaag aggtcaagaa ggacctgatt atcaagctgc     6660

ccaagtactc cctctttgag ctcgagaacg gccgaaagcg aatgctggct tccgctggtg     6720

agctgcagaa gggcaacgag ctcgctctgc cctccaagta cgtcaacttc ctctacctgg     6780

cctcccacta cgagaagctc aagggctccc ccgaggacaa cgagcagaag cagctgttcg     6840

ttgagcagca caagcactac ctcgacgaga tcatcgagca gatctccgag ttctccaagc     6900

gagtcatcct cgctgacgcc aaccttgata aggttctctc tgcttacaac aagcaccggg     6960

acaagcccat ccgagagcag gccgagaata tcatccacct cttcactctc accaacctcg     7020

gcgctcctgc tgccttcaag tacttcgaca ccaccattga ccgaaagagg tacacctcca     7080

ccaaggaagt cctcgacgcc accctgatcc accagtccat caccggcctc tacgaaaccc     7140

gaatcgacct ctcccagctc ggcggtgact ctcgagccga ccccaagaag aagcgaaaag     7200

tctaaatatc cgaagatcaa gagcgaagca agttgtaagt ccaggacatg tttcccgccc     7260

acgcgagtga tttataacac ctctcttttt tgacacccgc tcgccttgaa attcatgtca     7320

cataaattat agtcaacgac gtttgaataa cttgtcttgt agttcgatga tgatcatatg     7380

attacattaa tagtaattac tgtatttgat atatatacta attacaatag tacatattag     7440

aacatacaat agttagtgcc gtgaagtggc ttaaaatacc gcgagtcgat tacgtaatat     7500

tattacctct tgcccatcga acgtacaagt actcctctgt tctctccttc ctttgctttg     7560

tgcacgaaga actgcggtca ggtgacacaa ctttttccat ctcagggtgt gtcgcgtgtg     7620

cttcatccaa actttagttg gggttcgggt tcgcgcgaga tgatcacgtg ccctgatttg     7680

gtgtcgtccc ccgtcgcgct gcgcacgtga tttatttatt tccggtggct gctgtctacg     7740

cggggccttc tctgcccttc tgtttcaacc ttcgggcggt tctcgtaacc agcagtagca     7800

atccatttcg aaactcaaag agctaaaaac gttaaacctc agcagtcgct cgacgaatgg     7860

gctgcggttg ggaagcccac gaggcctata gccagagcct cgagttgaca ggagcccaga     7920

cgccttttcc aacggcaact tttatataaa atggcaatgt attcatgcaa ttgcggccgt     7980

gtcaggttgg agacactgga ccacactctc cattgcttcc tgaggagatg gatcattgct     8040

agtgcatcta cgcgcagcaa tcccgcaagc tcgacaaccg tagatgggct ttggtgggcc     8100

aatcaattac gcaacccgca cgttaaattg tatgaggaag gaaggccacg gtacaaagtg     8160

ggtggtcttc acccagtggt tgttggtggc gtcatgcaga ccatgcattg gggatagcac     8220

agggttgggg tgtcttgtgg actcaatggg tgaaaggaga tggaaaaggg cggtgaaaag     8280

tggtagaatc gaaatccctg acgtcaattt ataaagtaaa atgcgtttct gccattttgc     8340

tcccctcctt ctttcgcaat cgcctcccca aaagttgtcg tggcagtaca catgcttgca     8400

tacaatgaag ctaatccggc ttgctcagta gttgctatat ccaggcatgg tgtgaaaccc     8460

ctcaaagtat atataggagc ggtgagcccc agtctggggt cttttctctc catctcaaaa     8520

ctactttctc acaatgacga gactgatgag tccgtgagga cgaaacgagt aagctcgtcg     8580

gtggcctgga ttcgagtggg ttttagagct agaaatagca agttaaaata aggctagtcc     8640

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg gccggcatgg tcccagcctc     8700

ctcgctggcg ccggctgggc aacatgcttc ggcatggcga atgggactaa acttcgagct     8760

aatccagtag cttacgttac ccaggggcag gtcaactggc tagccacgag tctgtcccag     8820

gtcgcaattt agtgtaataa acaatatata tattgagtct aaagggaatt gtagctattg     8880

tgattgtgtg attttcgtct tgctggttct tattgtgtcc cattcgtttc atcctgatga     8940

ggacccctgg aaccggtgtt ttcttagtct ctgcaatcgc tagtcttgtt gctatgacag     9000

ttgcgtcgac actattcagg tcatctatcg gttattctga tattataata cctccggatc     9060

gatgtacctg atttatactt gcagcaatgt ttacttctta tcgttggacc ccgtcttcaa     9120

ttacacttcc caactgggaa cacccctctt tatcgaccca ttttaggtaa tttaccctag     9180

cccattgtct ccataaggaa tattacccta acccacagtc cagggtgccc aggtccttct     9240

ttggccaaat tttaacttcg gtcctatggc acagcggtag cgcgtgagat tgcaaatctt     9300

aaggtcccga gttcgaatct cggtgggacc tagttgaaaa atacctctaa tgcgccgatg     9360

gtttagtggt aaaatccatc gttgccatcg atgggccccc ggttcgattc cgggtcggcg     9420

caggttgacg tttacaccca ctctatcgga ggttttagag ctagaaatag caagttaaaa     9480

taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttttcgc     9540

gatacacgaa tgtgatacgg atcaaagtaa gcaggactac gataagataa cgaatgcggt     9600

gcagtccatg tcgattaggt atagatacat ttattttgtg ttatgttaca ttttgggggg     9660

atactgtcct acttgtagta cctacttgta gtggcgcgtc tattcctttg ccctcggacg     9720

agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac     9780

ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat     9840

tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg     9900

atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag     9960

ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct    10020

ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc    10080

aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg    10140

cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg    10200

cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc    10260

agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa    10320

tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc    10380

gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg    10440

tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc    10500

cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc    10560

atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc    10620

acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat    10680

cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatggg tacctgagaa    10740

catttttgtg tctaggtgtt tgtgtttgga ctgcgatcag tgaagaaaag aagaggaaaa    10800

attgtgcaag aaattttgct ttcaagactt ggctgatgca gcagggtaac tctgggacac    10860

agacctatgt ttgtggttaa actcaatgca cgtggtacgt gcgtggagcg cttacccatc    10920

caagggtgtg gacatggaac cgacggtccg tggagttgtg taatgtcatt ttggcgactc    10980

ttgaagcaag gctataaaaa aattgtgtgg cttgagtctt atcgagctcg gtcactacaa    11040

gagttaatct tcctgtctca ggcagacagg tcaggcaggg ttacttttgg gtgtgctgta    11100

actcactgta tggccgttag tgcgcataga cgttgtacat actggaccga attgtagcgt    11160

gctcaatagg gccaataaag ctattgtagg gatccgaatt ttcagaacct aatttatctg    11220

ttacccggcc tgtggctcgc acagcttaaa aatggtcaaa ctttcccctt cttgtctttt    11280

tttcctcaca ttcatcaggt tcttgtcttg atctttcaag tgagtattaa ttaccgacct    11340

tggttcttca ttgggagagc attggaagcc gtggtgcagc aaccacaaaa cggttcttcc    11400

ccttcgatac cttcttgcct gcctttcaat acaagtcggc tcgattagcg gtggtcgccc    11460

ccgccagcgg agaacatgga actaacccag aatgagagct aagtggagaa agaagagagt    11520

cagacgactc aagcgaaagc gccgcaaggt ccgagctcga tccaaataag cggtttttaa    11580

cggagattta acactaaatc gaagaacttt tcccgtttca tttgcgaatg agctcgttaa    11640

caaaatcccc cagttttttt atccagctgt aaggattgac attagtaatg aattattgtt    11700

tggtatattt aaatctgtag ttcctttctg tccgtgtcgg caactgtcgt actcgtgatt    11760

tacttgtatt gacgaatact tactgtagcg cactctgctg ctactggtcg taaggatgtg    11820

ctatttcggt gtatggtggg ttttttgggg gtcggaaccg aagactgtta cacgggcacg    11880

gctcgttgtg tacacgcaca gagctcttgc gagtcatgtt gtagctagct cgtcgtgttc    11940

aggaactgtt cgatggttcg gagagagtcg ccgcccagaa catacgcgca ccgatgtcag    12000

cagacagcct tattacaagt atattcaagc aagtatatcc gtagggtgcg ggtgatttgg    12060

atctaaggtt cgtactcaac actcacgagc agcttgccta tgttacatcc ttttatcaga    12120

cataacataa ttggagttta cttacacacg gggtgtacct gtatgagcac cacctacaat    12180

tgtagcactg gtacttgtac aaagaattta ttcgtacgaa tcacagggac ggccgccctc    12240

accgaaccag cgaatacctc agcggtcccc tgcagtgact caacaaagcg atatgaacat    12300

cttgcgatgg tatcctgctg atagttttta ctgtacaaac acctgtgtag ctccttctag    12360

catttttaag ttattcacac ctcaagggga gggataaatt aaataaattc caaaagcgaa    12420

gatcgagaaa ctaaattaaa attccaaaaa cgaagttgga acacaacccc ccgaaaaaaa    12480

acaacaaaca aaaaacccaa caaaataaac aaaaacaaaa taaatatata actaccagta    12540

tctgactaaa agttcaaata ctcgtactta caacaaatag aaatgagccg gccaaaattc    12600

tgcagaaaaa aatttcaaac aagtactggt ataattaaat taaaaaacac atcaaagtat    12660

cataacgtta gttattttat tttatttaat aaaagaaaac aacaagatgg gctcaaaact    12720

ttcaacttat acgatacata ccaaataaca atttagtatt tatctaagtg cttttcgtag    12780

ataatggaat acaaatggat atccagagta tacacatgga tagtatacac tgacacgaca    12840

attctgtatc tctttatgtt aactactgtg aggcattaaa tagagcttga tatataaaat    12900

gttacatttc acagtctgaa cttttgcaga ttacctaatt tggtaagata ttaattatga    12960

actgaaagtt gatggcatcc ctaaatttga tgaaagatga aattgtaaat gaggtggtaa    13020

aagagctaca gtcgttttgt tttgagatac catcatctct aacgaaatat ctattaaaaa    13080

tctcagtgtg atcatgagtc attgccatcc tggaaaatgt catcatggct gatatttcta    13140

actgtttact tgagataaat atatatttac aagaacttcc cttgaaatta atttagatat    13200

aaaatgtttg cgggcaagtt actacgagga ataaattata tctaga                   13246


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence used for Cas9 CRISPR targeting

<400>  13
acagcaggct gaacgaggat                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence used for Cas9 CRISPR targeting

<400>  14
tggaggcatg atcaacagcg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence used for Cas9 CRISPR targeting

<400>  15
tcactcctca gcctcccaag                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence used for Cas9 CRISPR targeting

<400>  16
ggtggcctgg attcgagtgg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence used for Cas9 CRISPR targeting

<400>  17
ttacacccac tctatcggag                                                   20


