                         SEQUENCE LISTING

<110>  Qatar Foundation for Education, Science and Community 
       Development
 
<120>  CRISPR-CAS9-SGRNA COMPLEX AND METHODS FOR CLONING APPLICATIONS

<130>  5600234.00622

<150>  US63/105,627
<151>  2020-10-26

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-Cas9-F1

<400>  1
ggcgccgaau uccagaucua guuuuagagc uaugcu                                 36


<210>  2
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-Cas9-F2

<400>  2
uaccgaagcu uucgucgacg guuuuagagc uaugcu                                 36


<210>  3
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-Cas9-R1

<400>  3
aagcuucggu accguagauc guuuuagagc uaugcu                                 36


<210>  4
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-Cas9-R2

<400>  4
uggauccaga ucuucucgag guuuuagagc uaugcu                                 36


<210>  5
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-I-2-BamHI-F1

<400>  5
gaacaucaaa gaugcauaug guuuuagagc uaugcu                                 36


<210>  6
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-I-2-BamHI-F2

<400>  6
aagaugcaua ugaggauccu guuuuagagc uaugcu                                 36


<210>  7
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-I-3-BbsI-F1

<400>  7
ucaacuucug cauugaaccg guuuuagagc uaugcu                                 36


<210>  8
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-I-3-BbsI-F1

<400>  8
aucagcccau guacguguuc guuuuagagc uaugcu                                 36


<210>  9
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-I-3-BbsI-R1

<400>  9
agaugauugu cuuaucauua guuuuagagc uaugcu                                 36


<210>  10
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-V-2-BbsI-R2

<400>  10
gauugucuua ucauuagggu guuuuagagc uaugcu                                 36


<210>  11
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-V-2-NotI-1

<400>  11
ccugcuguug gcggccgccc guuuuagagc uaugcu                                 36


<210>  12
<211>  36
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-V-2-NotI-2

<400>  12
cugauuauga ucuagagucg guuuuagagc uaugcu                                 36


<210>  13
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-1-BamHI-F primer

<400>  13
cacagcagga tccagtgagc aagggc                                            26


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-1-NotI-R1 primer

<400>  14
agggcggccg ccaacagcag ga                                                22


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-2-I-1-F primer

<400>  15
gaaggacggc ggccactacg ac                                                22


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-2-I-1-R primer

<400>  16
aaatctgctc attgcccttg gcg                                               23


<210>  17
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-3-KpnI-F primer

<400>  17
ctgactggta ccatgggaag ttcacatcat catcatcatc ac                          42


<210>  18
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-3-BbsI-R primer

<400>  18
gtctagagct aaggtcttct caagccagaa tcctttcgca cagc                        44


<210>  19
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-2-BamHI-F primer

<400>  19
attcgcggat ccatggtgag caagggcgag gag                                    33


<210>  20
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-2-EcoRI-R primer

<400>  20
aatgcagaat tcattatgaa gaacaatacc acaaaatgcc aaat                        44


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-3-I-2-F primer

<400>  21
cgtaacaact ccgccccatt ga                                                22


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-3-I-2-R primer

<400>  22
gcgtaaccac cacacccgcc                                                   20


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-3-I-3-F primer

<400>  23
atgggaagtt cacatcatca tcatcatcac                                        30


<210>  24
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-3-I-3-R primer

<400>  24
ctcaagccag aatcctttcg cacagc                                            26


<210>  25
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA sequence consisting of acrRNA sequence (n) aligned with 
       tracrRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  acrRNA sequence domain

<400>  25
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuagca uagcaaguua aaauaaggcu       60

aguccguuau caacuugaaa aaguggcacc gagucggugc uuu                        103


<210>  26
<211>  6477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of vector-1 with the multiple cloning-site insert 
       (V-1-MCS)

<400>  26
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gcttgccacc      600

atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc      660

tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      720

acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc      780

ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc      840

gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag      900

gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac      960

aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc     1020

atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag     1080

atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc     1140

tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac     1200

atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc     1260

cactccaccg gcgccgaatt ccagatctac ggtaccgaag ctttcgtcga cgaggtacca     1320

ctcgagaaga tctggatcca gtgagcaagg gcgaggagct gttcaccggg gtggtgccca     1380

tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg     1440

agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc     1500

ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct     1560

accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc     1620

aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt     1680

tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg     1740

gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg     1800

ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg     1860

gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc     1920

tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga     1980

agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg     2040

acgagctgta caagcaatgt actaactacg ctttgttgaa actcgctggc gatgttgaaa     2100

gtaaccccgg tcctgctagc atggcaccct gcatgctgct cctgctgttg gcggccgccc     2160

tggccccgac tcagacccgc gcgggcccac attcgctgag gtatttccac accgccgtgt     2220

cccggcccgg cctcgggaag ccccggttca tctctgtcgg ctacgtggac gacacgcagt     2280

tcgtgcgctt cgacagcgac gcggagaatc cgaggtatga gccgcgggtg cggtggatgg     2340

agcaggtgga gcccgagtat tgggagcgga acacgcagat cgccaagggc aatgagcaga     2400

ttttccgagt gaacctgagg accgcgctgc gctactacaa ccagagcgcg ggcggctctc     2460

acacgttcca acggatgtac ggctgtgagg tggggtcgga ctggcgcctc ctccgcgggt     2520

acgagcagta cgcatacgac ggctgcgatt acatcgccct gaacgaagac ctgaaaacgt     2580

ggacggcggc cgacatggcg gcgctgatca ccaaacacaa gtgggagcag gctggtgatg     2640

cagagagaga ccgggcctac ctggagggca cgtgcgtgga gtggctccgc agatacctgc     2700

agctcgggaa cgcgacgctg ccgcgcacag attccccaaa ggcccatgtg acccgtcaca     2760

gcagacctga agataaagtc accctgaggt gctgggccct gggcttctac cctgctgaca     2820

tcaccctgac ctggcagttg aatggggagg agctgaccca ggacatggag cttgtggaga     2880

ccaggcctgc aggggatgga accttccaga agtgggcatc tgtggtggtg cctcttggga     2940

aggagcagta ttacacatgc catgtgtacc atcaggggct gcctgagccc ctcaccctga     3000

gatgggagcc tcctccatcc actgtctcca acacggtaat cattgctgtt ctggttgtcc     3060

ttggagctgc aatagtcact ggagctgtgg tggcttttgt gatgaagatg agaaggagaa     3120

acacaggtgg aaaaggaggg taagcggccg cgactctaga tcataatcag ccataccaca     3180

tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat     3240

aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa     3300

agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt     3360

ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa     3420

attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa     3480

aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa     3540

caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca     3600

gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg     3660

taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc     3720

ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc     3780

aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca     3840

gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt     3900

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata     3960

atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta     4020

gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat     4080

tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc     4140

atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta     4200

actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca     4260

gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga     4320

ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga     4380

acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga     4440

ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg     4500

gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga     4560

ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt     4620

tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct     4680

gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct     4740

gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg     4800

agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca     4860

ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga     4920

tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt     4980

ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt     5040

ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct     5100

ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt     5160

cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca     5220

cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg     5280

gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct     5340

agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg     5400

gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt     5460

cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc     5520

cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca     5580

gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt     5640

gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc     5700

atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag     5760

atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa     5820

aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg     5880

aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag     5940

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg     6000

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga     6060

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc     6120

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc     6180

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga     6240

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt     6300

cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg     6360

aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac     6420

atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat        6477


<210>  27
<211>  6462
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of vector-2 (V-2)

<400>  27
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gcttgccacc      600

atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc      660

tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      720

acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc      780

ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc      840

gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag      900

gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac      960

aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc     1020

atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag     1080

atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc     1140

tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac     1200

atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc     1260

cactccaccg gcgccgaatt cgcgtccgcg ccatggccat ctacaagcag tcacagcagg     1320

atccagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg     1380

acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct     1440

acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca     1500

ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga     1560

agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct     1620

tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc     1680

tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc     1740

acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga     1800

acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg     1860

ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc     1920

actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg     1980

tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc     2040

aatgtactaa ctacgctttg ttgaaactcg ctggcgatgt tgaaagtaac cccggtcctg     2100

ctagcatggc accctgcatg ctgctcctgc tgttggcggc cgccctggcc ccgactcaga     2160

cccgcgcggg cccacattcg ctgaggtatt tccacaccgc cgtgtcccgg cccggcctcg     2220

ggaagccccg gttcatctct gtcggctacg tggacgacac gcagttcgtg cgcttcgaca     2280

gcgacgcgga gaatccgagg tatgagccgc gggtgcggtg gatggagcag gtggagcccg     2340

agtattggga gcggaacacg cagatcgcca agggcaatga gcagattttc cgagtgaacc     2400

tgaggaccgc gctgcgctac tacaaccaga gcgcgggcgg ctctcacacg ttccaacgga     2460

tgtacggctg tgaggtgggg tcggactggc gcctcctccg cgggtacgag cagtacgcat     2520

acgacggctg cgattacatc gccctgaacg aagacctgaa aacgtggacg gcggccgaca     2580

tggcggcgct gatcaccaaa cacaagtggg agcaggctgg tgatgcagag agagaccggg     2640

cctacctgga gggcacgtgc gtggagtggc tccgcagata cctgcagctc gggaacgcga     2700

cgctgccgcg cacagattcc ccaaaggccc atgtgacccg tcacagcaga cctgaagata     2760

aagtcaccct gaggtgctgg gccctgggct tctaccctgc tgacatcacc ctgacctggc     2820

agttgaatgg ggaggagctg acccaggaca tggagcttgt ggagaccagg cctgcagggg     2880

atggaacctt ccagaagtgg gcatctgtgg tggtgcctct tgggaaggag cagtattaca     2940

catgccatgt gtaccatcag gggctgcctg agcccctcac cctgagatgg gagcctcctc     3000

catccactgt ctccaacacg gtaatcattg ctgttctggt tgtccttgga gctgcaatag     3060

tcactggagc tgtggtggct tttgtgatga agatgagaag gagaaacaca ggtggaaaag     3120

gagggtaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta     3180

cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt     3240

gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca     3300

aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc     3360

aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt     3420

ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc     3480

aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt     3540

aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact     3600

acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg     3660

gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag     3720

aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac     3780

gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg     3840

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     3900

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     3960

gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc     4020

ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg     4080

tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag     4140

tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc     4200

gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc     4260

tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc     4320

aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca     4380

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac     4440

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt     4500

tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc     4560

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg     4620

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc     4680

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc     4740

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat     4800

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc     4860

cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca     4920

tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga     4980

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat     5040

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc     5100

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact     5160

ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc     5220

accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg     5280

atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact     5340

gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag     5400

aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg     5460

cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt     5520

tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg     5580

gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat     5640

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     5700

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     5760

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     5820

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     5880

agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc     5940

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     6000

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     6060

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     6120

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     6180

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     6240

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     6300

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     6360

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg     6420

ttatcccctg attctgtgga taaccgtatt accgccatgc at                        6462


<210>  28
<211>  767
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of the insert-1 (I-1)

<400>  28
ggatccatgc ccaagccaat ggccgccaac tatctgaaga atcagcccat gtacgtgttc       60

aggaagacag agctgaagca ctccaagacc gagctgaatt tcaaggagtg gcagaaggcc      120

tttacagacg tgatgggcat ggatgagctg tacaaggagt ttggcaccga gaacctgtat      180

aaaacgagtg ccgtattgca gagtgggttt cggaaaatgg aaggctctgg cggcagcatg      240

gtgttcacac tggaggactt tgtgggcgat tggcggcaga ccgccggcta taatctggat      300

caggtgctgg agcagggcgg cgtgagctcc ctgttccaga acctgggcgt gagcgtgacc      360

cctatccagc ggatcgtgct gtccggcgag aacggcctga agatcgacat ccacgtgatc      420

atcccatacg agggcctgtc tggcgatcag atgggccaga tcgagaagat cttcaaggtg      480

gtgtaccccg tggacgatca ccacttcaaa gtgatcctgc actatggcac cctggtcatc      540

gacggcgtga cccccaatat gatcgattat ttcggccggc cttacgaggg catcgccgtg      600

tttgacggca agaagatcac cgtgacaggc accctgtgga acggcaataa gatcatcgac      660

gagagactga tcaaccccga tggctccctg ctgttcaggg tgactattaa cggagtgact      720

ggctggcggc tgtgcgaaag gattctggct tgactcgagg cggccgc                    767


<210>  29
<211>  906
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pV-2-I-1 sequenced with pV-2-I-1-F primer

<400>  29
caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca       60

gtacgagcgc gccgagggcc gccactccac cggcgccgaa ttcgcgtccg cgccatggcc      120

atctacaagc agtcacagca ggatccatgc ccaagccaat ggccgccaac tatctgaaga      180

atcagcccat gtacgtgttc aggaagacag agctgaagca ctccaagacc gagctgaatt      240

tcaaggagtg gcagaaggcc tttacagacg tgatgggcat ggatgagctg tacaaggagt      300

ttggcaccga gaacctgtat aaaacgagtg ccgtattgca gagtgggttt cggaaaatgg      360

aaggctctgg cggcagcatg gtgttcacac tggaggactt tgtgggcgat tggcggcaga      420

ccgccggcta taatctggat caggtgctgg agcagggcgg cgtgagctcc ctgttccaga      480

acctgggcgt gagcgtgacc cctatccagc ggatcgtgct gtccggcgag aacggcctga      540

agatcgacat ccacgtgatc atcccatacg agggcctgtc tggcgatcag atgggccaga      600

tcgagaagat cttcaaggtg gtgtaccccg tggacgatca ccacttcaaa gtgatcctgc      660

actatggcac cctggtcatc gacggcgtga cccccaatat gatcgattat ttcggccggc      720

cttacgaggg catcgccgtg tttgacggca gaagatcacc gtgacaggca ccctgtggaa      780

cggcatagat catcgacgag agactgatca acccgatggc tcctgctgtc aggtgactat      840

tacggagtga ctgctgcgct gtgcgaaagg atctggcttg actcgagcgc cgcctgcccc      900

gactca                                                                 906


<210>  30
<211>  876
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-2-I-1 clone using pV-2-I-1-R primer

<400>  30
ttcaggaaga cagagctgaa gcactccaag accgagctga atttcaagga gtggcagaag       60

gccttacaga cgtgatgggc atggatgagc tgtacaagga gtttggcacc gagaacctgt      120

ataaaacgag tgccgtattg cagagtgggt ttcggaaaat ggaaggctct ggcggcagca      180

tggtgttcac actggaggac tttgtgggcg attggcggca gaccgccggc tataatctgg      240

atcaggtgct ggagcagggc ggcgtgagct ccctgttcca gaacctgggc gtgagcgtga      300

cccctatcca gcggatcgtg ctgtccggcg agaacggcct gaagatcgac atccacgtga      360

tcatcccata cgagggcctg tctggcgatc agatgggcca gatcgagaag atcttcaagg      420

tggtgtaccc cgtggacgat caccacttca aagtgatcct gcactatggc accctggtca      480

tcgacggcgt gacccccaat atgatcgatt atttcggccg gccttacgag ggcatcgccg      540

tgtttgacgg caagaagatc accgtgacag gcaccctgtg gaacggcaat aagatcatcg      600

acgagagact gatcaacccc gatggctccc tgctgttcag ggtgactatt aacggagtga      660

ctggctggcg gctgtgcgaa aggattctgg cttgactcga ggcggccgcc ctggccccga      720

ctcagacccg cgcgggccca cattcgctga ggtatttcca caccgccgtg tcccggcccg      780

gcctcgggaa gccccggttc atctctgtcg gctacgtgga cgacacgcag ttcgtgcgct      840

tcgacagcga cgcggagaat ccgaggtatg agccgc                                876


<210>  31
<211>  1218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of the insert-2 (I-2)

<400>  31
ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag       60

ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc      120

acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg      180

cccaccctcg tgaccaccct gagctacggc gtgcagtgct tcagccgcta ccccgaccac      240

atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc      300

atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac      360

accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg      420

gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag      480

aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag      540

ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac      600

aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac      660

atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac      720

aagtccggat caagcttaat ttctagtcat ttggatgtca cagccttatg tcacaaaatt      780

ttcttgcata tccatggact gatatctgct gaccgctatt ccctgttcct tgtctgtgaa      840

gacagctcca atgacaagtt tcttatcagc cgcctctttg atgttgctga aggttcaaca      900

ctggaagaag tttcaaataa ctgtatccgc ttagaatgga acaaaggcat tgtgggacat      960

gtggcagcgc ttggtgagcc cttgaacatc aaagatgcat atgaggatcc tcggttcaat     1020

gcagaagttg accaaattac aggctacaag acacaaagca ttctttgtat gccaattaag     1080

aatcataggg aagaggttgt tggtgtagcc caggccatca acaagaaatc aggaaacggt     1140

gggacattta ctgaaaaaga tgaaaaggac tttgctgctt atttggcatt ttgtggtatt     1200

gttcttcata atgaattc                                                   1218


<210>  32
<211>  5432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of vector-3 (V-3)

<400>  32
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc      960

agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca     1020

gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc     1080

ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg     1140

cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg     1200

gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag     1260

gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta     1320

agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg     1380

cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa     1440

gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc     1500

aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt     1560

cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca     1620

acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc     1680

tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg     1740

tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc     1800

atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga     1860

agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc     1920

atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt     1980

tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga     2040

ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc     2100

ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac     2160

gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca     2220

atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt     2280

gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg     2340

tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga     2400

agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct     2460

cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg     2520

gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg     2580

gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc     2640

gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat     2700

ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac     2760

tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt     2820

gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct     2880

cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc     2940

tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca     3000

ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga     3060

tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag     3120

cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt     3180

cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac     3240

cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt     3300

gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg     3360

gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt     3420

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt     3480

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     3540

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     3600

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     3660

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     3720

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     3780

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     3840

ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg     3900

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     3960

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     4020

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     4080

tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc     4140

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     4200

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     4260

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     4320

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     4380

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     4440

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     4500

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     4560

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     4620

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     4680

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     4740

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     4800

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     4860

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     4920

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     4980

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     5040

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     5100

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     5160

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     5220

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     5280

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt     5340

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc     5400

gcacatttcc ccgaaaagtg ccacctgacg tc                                   5432


<210>  33
<211>  821
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-2 using primer pV-3-I-2-F1

<400>  33
taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc       60

gagctcggat ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg      120

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc      180

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg      240

ccctggccca ccctcgtgac caccctgagc tacggcgtgc agtgcttcag ccgctacccc      300

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag      360

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag      420

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac      480

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac      540

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc      600

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg      660

cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc      720

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag      780

ctgtacagtc cggatcagct taatttctag tcattggatg t                          821


<210>  34
<211>  934
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-2 using primer pV-3-I-2-F2

<400>  34
gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac       60

ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt      120

cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gatcaagctt      180

aatttctagt catttggatg tcacagcctt atgtcacaaa attttcttgc atatccatgg      240

actgatatct gctgaccgct attccctgtt ccttgtctgt gaagacagct ccaatgacaa      300

gtttcttatc agccgcctct ttgatgttgc tgaaggttca acactggaag aagtttcaaa      360

taactgtatc cgcttagaat ggaacaaagg cattgtggga catgtggcag cgcttggtga      420

gcccttgaac atcaaagatg catatgagga tcctcggttc aatgcagaag ttgaccaaat      480

tacaggctac aagacacaaa gcattctttg tatgccaatt aagaatcata gggaagaggt      540

tgttggtgta gcccaggcca tcaacaagaa atcaggaaac ggtgggacat ttactgaaaa      600

agatgaaaag gactttgctg cttatttggc attttgtggt attgttcttc ataatgaatt      660

ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg      720

atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc      780

ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc      840

atcgcattgt ctgagtaggt gtcattctat tctggggggg tgggggtggg gcaggacagc      900

aaggggagga ttgggaagac aataagcagg catg                                  934


<210>  35
<211>  1354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   DNA sequence of the insert-3 (I-3)

<400>  35
ggtaccatgg gaagttcaca tcatcatcat catcactcat caggactggt gccacggggg       60

tctcacatgg tctccaaggg ggaagaggac aacatggcct ctctgcctgc cacacacgag      120

ctgcacatct tcggcagcat caatggcgtg gactttgata tggtgggcca gggcacaggc      180

aacccaaatg acggctacga ggagctgaac ctgaagagca ccaagggcga tctgcagttc      240

tccccttgga tcctggtgcc acacatcggc tatggctttc accagtatct gccctaccct      300

gacggcatgt cccccttcca ggccgccatg gtggatggct ctggctacca ggtgcacagg      360

accatgcagt ttgaggacgg cgcctccctg acagtgaatt accggtatac ctacgagggc      420

tctcacatca agggcgaggc ccaggtgaag ggcacaggct tcccagccga tggccccgtg      480

atgacaaact ctctgaccgc cgccgactgg tgccggagca agaagaccta ccctaatgat      540

aagacaatca tctccacctt taagtggtct tataccacag gcaacggcaa gcggtacaga      600

agcacagcca gaaccacata tacctttgcc aagccaatgg ccgccaacta tctgaagaat      660

cagcccatgt acgtgttcag gaagacagag ctgaagcact ccaagaccga gctgaatttc      720

aaggagtggc agaaggcctt tacagacgtg atgggcatgg atgagctgta caaggagttt      780

ggcaccgaga acctgtatga tgaggtcgac ggctctggcg gcagcatggt gttcacactg      840

gaggactttg tgggcgattg gcggcagacc gccggctata atctggatca ggtgctggag      900

cagggcggcg tgagctccct gttccagaac ctgggcgtga gcgtgacccc tatccagcgg      960

atcgtgctgt ccggcgagaa cggcctgaag atcgacatcc acgtgatcat cccatacgag     1020

ggcctgtctg gcgatcagat gggccagatc gagaagatct tcaaggtggt gtaccccgtg     1080

gacgatcacc acttcaaagt gatcctgcac tatggcaccc tggtcatcga cggcgtgacc     1140

cccaatatga tcgattattt cggccggcct tacgagggca tcgccgtgtt tgacggcaag     1200

aagatcaccg tgacaggcac cctgtggaac ggcaataaga tcatcgacga gagactgatc     1260

aaccccgatg gctccctgct gttcagggtg actattaacg gagtgactgg ctggcggctg     1320

tgcgaaagga ttctggcttg agaagacctt agca                                 1354


<210>  36
<211>  712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-3 using primer pV-3-I-3-F1

<400>  36
gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga       60

gacccaagct ggctagcgtt taaacttaag cttggtacca tgggaagttc acatcatcat      120

catcatcact catcaggact ggtgccacgg gggtctcaca tggtctccaa gggggaagag      180

gacaacatgg cctctctgcc tgccacacac gagctgcaca tcttcggcag catcaatggc      240

gtggactttg atatggtggg ccagggcaca ggcaacccaa atgacggcta cgaggagctg      300

aacctgaaga gcaccaaggg cgatctgcag ttctcccctt ggatcctggt gccacacatc      360

ggctatggct ttcaccagta tctgccctac cctgacggca tgtccccctt ccaggccgcc      420

atggtggatg gctctggcta ccaggtgcac aggaccatgc agtttgagga cggcgcctcc      480

ctgacagtga attaccggta tacctacgag ggctctcaca tcaagggcga ggcccaggtg      540

aagggcacag gcttcccagc cgatggcccc gtgatgacaa actctctgac cgccgccgac      600

tggtgccgga gcaagaagac ctaccctaat gataagacaa tcatctccac ctttaagtgg      660

tcttatacca caggcaacgg caagcggtac agaagcacag ccagaaccac at              712


<210>  37
<211>  639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-3 using primer pV-3-I-3-F2

<400>  37
cggtggaatt accggtatac ctacgagggg taacacrtca agggcgaggc ccaggtgaag       60

ggcacaggct tcccagccga tggccccgtg atgacaaact ctctgaccgc cgccgactgg      120

tgccggagca agaagaccta ccctaatgat aagacaatca tctccacctt taagtggtct      180

tataccacag gcaacggcaa gcggtacaga agcacagcca gaaccacata tacctttgcc      240

aagccaatgg ccgccaacta tctgaagaat cagcccatgt acgtgttcag gaagacagag      300

ctgaagcact ccaagaccga gctgaatttc aaggagtggc agaaggcctt tacagacgtg      360

atgggcatgg atgagctgta caaggagttt ggcaccgaga acctgtatgc agtgctccaa      420

agcggatttc gcggctctgg cggcagcatg gtgttcacac tggaggactt tgtgggcgat      480

tggcggcaga ccgccggcta taatctggat caggtgctgg agcagggcgg cgtgagctcc      540

ctgttccaga acctgggcgt gagcgtgacc cctatccagc ggatcgtgct gtccggcgag      600

aacggcctga agatcgacat ccacgtgatc atcccatac                             639


<210>  38
<211>  859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-3 using primer pV-3-I-3-F3

<400>  38
cacacatcgg ctatggcttt caccagtatc tgccctaccc tgacggcatg tcccccttcc       60

aggccgccat ggtggatggc tctggctacc aggtgcacag gaccatgcag tttgaggacg      120

gcgcctccct gacagtgaat taccggtata cctacgaggg ctctcacatc aagggcgagg      180

cccaggtgaa gggcacaggc ttcccagccg atggccccgt gatgacaaac tctctgaccg      240

ccgccgactg gtgccggagc aagaagacct accctaatga taagacaatc atctccacct      300

ttaagtggtc ttataccaca ggcaacggca agcggtacag aagcacagcc agaaccacat      360

atacctttgc caagccaatg gccgccaact atctgaagaa tcagcccatg tacgtgttca      420

ggaagacaga gctgaagcac tccaagaccg agctgaattt caaggagtgg cagaaggcct      480

ttacagacgt gatgggcatg gatgagctgt acaaggagtt tggcaccgag aacctgtatg      540

atgaggtcga cggctctggc ggcagcatgg tgttcacact ggaggacttt gtgggcgatt      600

ggcggcagac cgccggctat aatctggatc aggtgctgga gcagggcggc gtgagctccc      660

tgttccagaa cctgggcgtg agcgtgaccs ctatccagcg gatcgtgctg tccggcgaga      720

acggcctgaa gatcgacatc cacgtgatca tcccatacga gggcctgtct ggcgatcaga      780

tgggccagat cgagaagatc ttcaaggtgt gtacccgtga cgatcacact tcaagtgatc      840

tgcactatgg caccstgtc                                                   859


<210>  39
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of pV-3-I-3 using primer pV-3-I-3-F4

<400>  39
tcgagatcag catgtacggt tcagagcaga gctgagcatc cagacgagct gattcaggag       60

tgcagagctt acagacgtga tgggcatgat gagctgtaca gagtttggca cgagacctgt      120

atgcagtgct ccaaagcgat ttcgcggctc tggcggcagc atggtgtcac actggaggac      180

tttgtgggcg attggcggca gaccgccggc tataatctgg atcaggtgct ggagcagggc      240

ggcgtgagct ccctgttcca gaacctgggc gtgagcgtga cccctatcca gcggatcgtg      300

ctgtccggcg agaacggcct gaagatcgac atccacgtga tcatcccata cgagggcctg      360

tctggcgatc agatgggcca gatcgagaag atcttcaagg tggtgtaccc cgtggacgat      420

caccacttca aagtgatcct gcactatggc accctggtca tcgacggcgt gacccccaat      480

atgatcgatt atttcggccg gccttacgag ggcatcgccg tgtttgacgg caagaagatc      540

accgtgacag gcaccctgtg gaacggcaat aagatcatcg acgagagact gatcaacccc      600

gatggctccc tgctgttcag ggtgactatt aacggagtga ctggctggcg gctgtgcgaa      660

aggattctgg cttgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact      720

gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg      780

gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg      840

agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg      900

gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaa         957


<210>  40
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mulitple cloning site targt DNA of V-1

<400>  40
cactccaccg gcgccgaatt ccagatctac ggtaccgaag ctttcgtcga cgaggtacca       60

ctcgagaaga tctggatcca gtgagcaa                                          88


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-sgRNA-NotI-1

<400>  41
ttttcctccc attcgccggc gct                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-sgRNA-NotI-2

<400>  42
aggacgacaa ccgccgcgcg gga                                               23


<210>  43
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protospacer adjacent motif (PAM) targeting BamHI

<400>  43
ccttgaacat caaagatgca tatgaggatc ccggttcaat gcagaagttg acca             54


<210>  44
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proto-spacer adjacent motif targeting BbsI site 1

<400>  44
gcaagaagac ctaccctaat gataagacaa tcatctccac cttt                        44


<210>  45
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Proto-spacer adjacent motif targeting BbsI site 2

<400>  45
atctgaagaa tcagcccatg tacgtgttca ggaagacaga gctgaagc                    48


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-gRNA-BamHI-R1

<400>  46
tcaacttctg cattgaaccg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-SRNA-BbsI-R3

<400>  47
agatgattgt cttatcatta                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-sRNA-BbsI-F1

<400>  48
atcagcccat gtacgtgttc                                                   20


