                         SEQUENCE LISTING

<110>  THE GENERAL HOSPITAL CORPORATION
 
<120>  KITS, REAGENTS AND METHODS FOR THE ASSESSMENT OF LIVER DISEASES

<130>  G11718-00419-SSS

<150>  US 63/107,730
<151>  2020-10-30

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Modified GRF peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tyr residue is linked to an hexenoyl-trans-3 moiety

<220>
<221>  MOD_RES
<222>  (44)..(44)
<223>  AMIDATION

<400>  1

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Ser Asn Gln Glu Arg Gly Ala Arg Ala Arg Leu 
        35                  40                  


<210>  2
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GRF peptide


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa = Tyr or His

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa = Val or Ala

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa = Asn or Ser

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa = Val or Ile

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  Xaa = Ala or Gly

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Xaa = Ser or Tyr

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa = Gln or His

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Xaa = Asp or Glu

<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  Xaa = Met or Ile or Nle

<220>
<221>  VARIANT
<222>  (28)..(28)
<223>  Xaa = Ser or Asn

<220>
<221>  VARIANT
<222>  (30)..(30)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Xaa = any amino acid or is absent

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (33)..(33)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (34)..(34)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (35)..(35)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (37)..(37)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (38)..(38)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (39)..(39)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  Xaa = any amino acid or is absent

<220>
<221>  VARIANT
<222>  (44)..(44)
<223>  Xaa = any amino acid or is absent

<400>  2

Xaa Xaa Asp Ala Ile Phe Tyr Xaa Ser Tyr Arg Lys Xaa Leu Xaa Gln 
1               5                   10                  15      


Leu Xaa Ala Arg Lys Leu Leu Xaa Xaa Ile Xaa Xaa Arg Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  


<210>  3
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence corresponding to positions 30 to 44 of the 
       human GRF

<400>  3

Gln Gln Gly Glu Ser Asn Gln Glu Arg Gly Ala Arg Ala Arg Leu 
1               5                   10                  15  


<210>  4
<211>  44
<212>  PRT
<213>  Homo sapiens

<400>  4

Tyr Ala Asp Ala Ile Phe Thr Asn Ser Tyr Arg Lys Val Leu Gly Gln 
1               5                   10                  15      


Leu Ser Ala Arg Lys Leu Leu Gln Asp Ile Met Ser Arg Gln Gln Gly 
            20                  25                  30          


Glu Ser Asn Gln Glu Arg Gly Ala Arg Ala Arg Leu 
        35                  40                  


<210>  5
<211>  232
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          


Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              


Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  


Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  


Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      


Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         


Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             


Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Lys Lys Ser Val 
    130                 135                 140                 


Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys Arg Lys Lys Ser Arg Tyr 
145                 150                 155                 160 


Lys Ser Trp Ser Val Tyr Val Gly Ala Arg Cys Cys Leu Met Pro Trp 
                165                 170                 175     


Ser Leu Pro Gly Pro His Pro Cys Gly Pro Cys Ser Glu Arg Arg Lys 
            180                 185                 190         


His Leu Phe Val Gln Asp Pro Gln Thr Cys Lys Cys Ser Cys Lys Asn 
        195                 200                 205             


Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn Glu Arg Thr 
    210                 215                 220                 


Cys Arg Cys Asp Lys Pro Arg Arg 
225                 230         


<210>  6
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      


Tyr Leu His His Ala Lys Trp Ser Gln Ala 
            20                  25      


<210>  7
<211>  206
<212>  PRT
<213>  Homo sapiens

<400>  7

Ala Pro Met Ala Glu Gly Gly Gly Gln Asn His His Glu Val Val Lys 
1               5                   10                  15      


Phe Met Asp Val Tyr Gln Arg Ser Tyr Cys His Pro Ile Glu Thr Leu 
            20                  25                  30          


Val Asp Ile Phe Gln Glu Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys 
        35                  40                  45              


Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly Cys Cys Asn Asp Glu 
    50                  55                  60                  


Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr Met Gln Ile 
65                  70                  75                  80  


Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu Met Ser Phe 
                85                  90                  95      


Leu Gln His Asn Lys Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg 
            100                 105                 110         


Gln Glu Lys Lys Ser Val Arg Gly Lys Gly Lys Gly Gln Lys Arg Lys 
        115                 120                 125             


Arg Lys Lys Ser Arg Tyr Lys Ser Trp Ser Val Tyr Val Gly Ala Arg 
    130                 135                 140                 


Cys Cys Leu Met Pro Trp Ser Leu Pro Gly Pro His Pro Cys Gly Pro 
145                 150                 155                 160 


Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr Cys 
                165                 170                 175     


Lys Cys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu 
            180                 185                 190         


Glu Leu Asn Glu Arg Thr Cys Arg Cys Asp Lys Pro Arg Arg 
        195                 200                 205     


<210>  8
<211>  390
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Pro Pro Ser Gly Leu Arg Leu Leu Leu Leu Leu Leu Pro Leu Leu 
1               5                   10                  15      


Trp Leu Leu Val Leu Thr Pro Gly Arg Pro Ala Ala Gly Leu Ser Thr 
            20                  25                  30          


Cys Lys Thr Ile Asp Met Glu Leu Val Lys Arg Lys Arg Ile Glu Ala 
        35                  40                  45              


Ile Arg Gly Gln Ile Leu Ser Lys Leu Arg Leu Ala Ser Pro Pro Ser 
    50                  55                  60                  


Gln Gly Glu Val Pro Pro Gly Pro Leu Pro Glu Ala Val Leu Ala Leu 
65                  70                  75                  80  


Tyr Asn Ser Thr Arg Asp Arg Val Ala Gly Glu Ser Ala Glu Pro Glu 
                85                  90                  95      


Pro Glu Pro Glu Ala Asp Tyr Tyr Ala Lys Glu Val Thr Arg Val Leu 
            100                 105                 110         


Met Val Glu Thr His Asn Glu Ile Tyr Asp Lys Phe Lys Gln Ser Thr 
        115                 120                 125             


His Ser Ile Tyr Met Phe Phe Asn Thr Ser Glu Leu Arg Glu Ala Val 
    130                 135                 140                 


Pro Glu Pro Val Leu Leu Ser Arg Ala Glu Leu Arg Leu Leu Arg Leu 
145                 150                 155                 160 


Lys Leu Lys Val Glu Gln His Val Glu Leu Tyr Gln Lys Tyr Ser Asn 
                165                 170                 175     


Asn Ser Trp Arg Tyr Leu Ser Asn Arg Leu Leu Ala Pro Ser Asp Ser 
            180                 185                 190         


Pro Glu Trp Leu Ser Phe Asp Val Thr Gly Val Val Arg Gln Trp Leu 
        195                 200                 205             


Ser Arg Gly Gly Glu Ile Glu Gly Phe Arg Leu Ser Ala His Cys Ser 
    210                 215                 220                 


Cys Asp Ser Arg Asp Asn Thr Leu Gln Val Asp Ile Asn Gly Phe Thr 
225                 230                 235                 240 


Thr Gly Arg Arg Gly Asp Leu Ala Thr Ile His Gly Met Asn Arg Pro 
                245                 250                 255     


Phe Leu Leu Leu Met Ala Thr Pro Leu Glu Arg Ala Gln His Leu Gln 
            260                 265                 270         


Ser Ser Arg His Arg Arg Ala Leu Asp Thr Asn Tyr Cys Phe Ser Ser 
        275                 280                 285             


Thr Glu Lys Asn Cys Cys Val Arg Gln Leu Tyr Ile Asp Phe Arg Lys 
    290                 295                 300                 


Asp Leu Gly Trp Lys Trp Ile His Glu Pro Lys Gly Tyr His Ala Asn 
305                 310                 315                 320 


Phe Cys Leu Gly Pro Cys Pro Tyr Ile Trp Ser Leu Asp Thr Gln Tyr 
                325                 330                 335     


Ser Lys Val Leu Ala Leu Tyr Asn Gln His Asn Pro Gly Ala Ser Ala 
            340                 345                 350         


Ala Pro Cys Cys Val Pro Gln Ala Leu Glu Pro Leu Pro Ile Val Tyr 
        355                 360                 365             


Tyr Val Gly Arg Lys Pro Lys Val Glu Gln Leu Ser Asn Met Ile Val 
    370                 375                 380                 


Arg Ser Cys Lys Cys Ser 
385                 390 


<210>  9
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Pro Pro Ser Gly Leu Arg Leu Leu Leu Leu Leu Leu Pro Leu Leu 
1               5                   10                  15      


Trp Leu Leu Val Leu Thr Pro Gly Arg Pro Ala Ala Gly 
            20                  25                  


<210>  10
<211>  249
<212>  PRT
<213>  Homo sapiens

<400>  10

Leu Ser Thr Cys Lys Thr Ile Asp Met Glu Leu Val Lys Arg Lys Arg 
1               5                   10                  15      


Ile Glu Ala Ile Arg Gly Gln Ile Leu Ser Lys Leu Arg Leu Ala Ser 
            20                  25                  30          


Pro Pro Ser Gln Gly Glu Val Pro Pro Gly Pro Leu Pro Glu Ala Val 
        35                  40                  45              


Leu Ala Leu Tyr Asn Ser Thr Arg Asp Arg Val Ala Gly Glu Ser Ala 
    50                  55                  60                  


Glu Pro Glu Pro Glu Pro Glu Ala Asp Tyr Tyr Ala Lys Glu Val Thr 
65                  70                  75                  80  


Arg Val Leu Met Val Glu Thr His Asn Glu Ile Tyr Asp Lys Phe Lys 
                85                  90                  95      


Gln Ser Thr His Ser Ile Tyr Met Phe Phe Asn Thr Ser Glu Leu Arg 
            100                 105                 110         


Glu Ala Val Pro Glu Pro Val Leu Leu Ser Arg Ala Glu Leu Arg Leu 
        115                 120                 125             


Leu Arg Leu Lys Leu Lys Val Glu Gln His Val Glu Leu Tyr Gln Lys 
    130                 135                 140                 


Tyr Ser Asn Asn Ser Trp Arg Tyr Leu Ser Asn Arg Leu Leu Ala Pro 
145                 150                 155                 160 


Ser Asp Ser Pro Glu Trp Leu Ser Phe Asp Val Thr Gly Val Val Arg 
                165                 170                 175     


Gln Trp Leu Ser Arg Gly Gly Glu Ile Glu Gly Phe Arg Leu Ser Ala 
            180                 185                 190         


His Cys Ser Cys Asp Ser Arg Asp Asn Thr Leu Gln Val Asp Ile Asn 
        195                 200                 205             


Gly Phe Thr Thr Gly Arg Arg Gly Asp Leu Ala Thr Ile His Gly Met 
    210                 215                 220                 


Asn Arg Pro Phe Leu Leu Leu Met Ala Thr Pro Leu Glu Arg Ala Gln 
225                 230                 235                 240 


His Leu Gln Ser Ser Arg His Arg Arg 
                245                 


<210>  11
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  11

Ala Leu Asp Thr Asn Tyr Cys Phe Ser Ser Thr Glu Lys Asn Cys Cys 
1               5                   10                  15      


Val Arg Gln Leu Tyr Ile Asp Phe Arg Lys Asp Leu Gly Trp Lys Trp 
            20                  25                  30          


Ile His Glu Pro Lys Gly Tyr His Ala Asn Phe Cys Leu Gly Pro Cys 
        35                  40                  45              


Pro Tyr Ile Trp Ser Leu Asp Thr Gln Tyr Ser Lys Val Leu Ala Leu 
    50                  55                  60                  


Tyr Asn Gln His Asn Pro Gly Ala Ser Ala Ala Pro Cys Cys Val Pro 
65                  70                  75                  80  


Gln Ala Leu Glu Pro Leu Pro Ile Val Tyr Tyr Val Gly Arg Lys Pro 
                85                  90                  95      


Lys Val Glu Gln Leu Ser Asn Met Ile Val Arg Ser Cys Lys Cys Ser 
            100                 105                 110         


<210>  12
<211>  554
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu 
1               5                   10                  15      


Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr 
            20                  25                  30          


Glu Glu Val Ser Glu Tyr Cys Ser His Met Ile Gly Ser Gly His Leu 
        35                  40                  45              


Gln Ser Leu Gln Arg Leu Ile Asp Ser Gln Met Glu Thr Ser Cys Gln 
    50                  55                  60                  


Ile Thr Phe Glu Phe Val Asp Gln Glu Gln Leu Lys Asp Pro Val Cys 
65                  70                  75                  80  


Tyr Leu Lys Lys Ala Phe Leu Leu Val Gln Asp Ile Met Glu Asp Thr 
                85                  90                  95      


Met Arg Phe Arg Asp Asn Thr Pro Asn Ala Ile Ala Ile Val Gln Leu 
            100                 105                 110         


Gln Glu Leu Ser Leu Arg Leu Lys Ser Cys Phe Thr Lys Asp Tyr Glu 
        115                 120                 125             


Glu His Asp Lys Ala Cys Val Arg Thr Phe Tyr Glu Thr Pro Leu Gln 
    130                 135                 140                 


Leu Leu Glu Lys Val Lys Asn Val Phe Asn Glu Thr Lys Asn Leu Leu 
145                 150                 155                 160 


Asp Lys Asp Trp Asn Ile Phe Ser Lys Asn Cys Asn Asn Ser Phe Ala 
                165                 170                 175     


Glu Cys Ser Ser Gln Asp Val Val Thr Lys Pro Asp Cys Asn Cys Leu 
            180                 185                 190         


Tyr Pro Lys Ala Ile Pro Ser Ser Asp Pro Ala Ser Val Ser Pro His 
        195                 200                 205             


Gln Pro Leu Ala Pro Ser Met Ala Pro Val Ala Gly Leu Thr Trp Glu 
    210                 215                 220                 


Asp Ser Glu Gly Thr Glu Gly Ser Ser Leu Leu Pro Gly Glu Gln Pro 
225                 230                 235                 240 


Leu His Thr Val Asp Pro Gly Ser Ala Lys Gln Arg Pro Pro Arg Ser 
                245                 250                 255     


Thr Cys Gln Ser Phe Glu Pro Pro Glu Thr Pro Val Val Lys Asp Ser 
            260                 265                 270         


Thr Ile Gly Gly Ser Pro Gln Pro Arg Pro Ser Val Gly Ala Phe Asn 
        275                 280                 285             


Pro Gly Met Glu Asp Ile Leu Asp Ser Ala Met Gly Thr Asn Trp Val 
    290                 295                 300                 


Pro Glu Glu Ala Ser Gly Glu Ala Ser Glu Ile Pro Val Pro Gln Gly 
305                 310                 315                 320 


Thr Glu Leu Ser Pro Ser Arg Pro Gly Gly Gly Ser Met Gln Thr Glu 
                325                 330                 335     


Pro Ala Arg Pro Ser Asn Phe Leu Ser Ala Ser Ser Pro Leu Pro Ala 
            340                 345                 350         


Ser Ala Lys Gly Gln Gln Pro Ala Asp Val Thr Gly Thr Ala Leu Pro 
        355                 360                 365             


Arg Val Gly Pro Val Arg Pro Thr Gly Gln Asp Trp Asn His Thr Pro 
    370                 375                 380                 


Gln Lys Thr Asp His Pro Ser Ala Leu Leu Arg Asp Pro Pro Glu Pro 
385                 390                 395                 400 


Gly Ser Pro Arg Ile Ser Ser Leu Arg Pro Gln Gly Leu Ser Asn Pro 
                405                 410                 415     


Ser Thr Leu Ser Ala Gln Pro Gln Leu Ser Arg Ser His Ser Ser Gly 
            420                 425                 430         


Ser Val Leu Pro Leu Gly Glu Leu Glu Gly Arg Arg Ser Thr Arg Asp 
        435                 440                 445             


Arg Arg Ser Pro Ala Glu Pro Glu Gly Gly Pro Ala Ser Glu Gly Ala 
    450                 455                 460                 


Ala Arg Pro Leu Pro Arg Phe Asn Ser Val Pro Leu Thr Asp Thr Gly 
465                 470                 475                 480 


His Glu Arg Gln Ser Glu Gly Ser Phe Ser Pro Gln Leu Gln Glu Ser 
                485                 490                 495     


Val Phe His Leu Leu Val Pro Ser Val Ile Leu Val Leu Leu Ala Val 
            500                 505                 510         


Gly Gly Leu Leu Phe Tyr Arg Trp Arg Arg Arg Ser His Gln Glu Pro 
        515                 520                 525             


Gln Arg Ala Asp Ser Pro Leu Glu Gln Pro Glu Gly Ser Pro Leu Thr 
    530                 535                 540                 


Gln Asp Asp Arg Gln Val Glu Leu Pro Val 
545                 550                 


<210>  13
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Thr Ala Pro Gly Ala Ala Gly Arg Cys Pro Pro Thr Thr Trp Leu 
1               5                   10                  15      


Gly Ser Leu Leu Leu Leu Val Cys Leu Leu Ala Ser Arg Ser Ile Thr 
            20                  25                  30          


<210>  14
<211>  522
<212>  PRT
<213>  Homo sapiens

<400>  14

Glu Glu Val Ser Glu Tyr Cys Ser His Met Ile Gly Ser Gly His Leu 
1               5                   10                  15      


Gln Ser Leu Gln Arg Leu Ile Asp Ser Gln Met Glu Thr Ser Cys Gln 
            20                  25                  30          


Ile Thr Phe Glu Phe Val Asp Gln Glu Gln Leu Lys Asp Pro Val Cys 
        35                  40                  45              


Tyr Leu Lys Lys Ala Phe Leu Leu Val Gln Asp Ile Met Glu Asp Thr 
    50                  55                  60                  


Met Arg Phe Arg Asp Asn Thr Pro Asn Ala Ile Ala Ile Val Gln Leu 
65                  70                  75                  80  


Gln Glu Leu Ser Leu Arg Leu Lys Ser Cys Phe Thr Lys Asp Tyr Glu 
                85                  90                  95      


Glu His Asp Lys Ala Cys Val Arg Thr Phe Tyr Glu Thr Pro Leu Gln 
            100                 105                 110         


Leu Leu Glu Lys Val Lys Asn Val Phe Asn Glu Thr Lys Asn Leu Leu 
        115                 120                 125             


Asp Lys Asp Trp Asn Ile Phe Ser Lys Asn Cys Asn Asn Ser Phe Ala 
    130                 135                 140                 


Glu Cys Ser Ser Gln Asp Val Val Thr Lys Pro Asp Cys Asn Cys Leu 
145                 150                 155                 160 


Tyr Pro Lys Ala Ile Pro Ser Ser Asp Pro Ala Ser Val Ser Pro His 
                165                 170                 175     


Gln Pro Leu Ala Pro Ser Met Ala Pro Val Ala Gly Leu Thr Trp Glu 
            180                 185                 190         


Asp Ser Glu Gly Thr Glu Gly Ser Ser Leu Leu Pro Gly Glu Gln Pro 
        195                 200                 205             


Leu His Thr Val Asp Pro Gly Ser Ala Lys Gln Arg Pro Pro Arg Ser 
    210                 215                 220                 


Thr Cys Gln Ser Phe Glu Pro Pro Glu Thr Pro Val Val Lys Asp Ser 
225                 230                 235                 240 


Thr Ile Gly Gly Ser Pro Gln Pro Arg Pro Ser Val Gly Ala Phe Asn 
                245                 250                 255     


Pro Gly Met Glu Asp Ile Leu Asp Ser Ala Met Gly Thr Asn Trp Val 
            260                 265                 270         


Pro Glu Glu Ala Ser Gly Glu Ala Ser Glu Ile Pro Val Pro Gln Gly 
        275                 280                 285             


Thr Glu Leu Ser Pro Ser Arg Pro Gly Gly Gly Ser Met Gln Thr Glu 
    290                 295                 300                 


Pro Ala Arg Pro Ser Asn Phe Leu Ser Ala Ser Ser Pro Leu Pro Ala 
305                 310                 315                 320 


Ser Ala Lys Gly Gln Gln Pro Ala Asp Val Thr Gly Thr Ala Leu Pro 
                325                 330                 335     


Arg Val Gly Pro Val Arg Pro Thr Gly Gln Asp Trp Asn His Thr Pro 
            340                 345                 350         


Gln Lys Thr Asp His Pro Ser Ala Leu Leu Arg Asp Pro Pro Glu Pro 
        355                 360                 365             


Gly Ser Pro Arg Ile Ser Ser Leu Arg Pro Gln Gly Leu Ser Asn Pro 
    370                 375                 380                 


Ser Thr Leu Ser Ala Gln Pro Gln Leu Ser Arg Ser His Ser Ser Gly 
385                 390                 395                 400 


Ser Val Leu Pro Leu Gly Glu Leu Glu Gly Arg Arg Ser Thr Arg Asp 
                405                 410                 415     


Arg Arg Ser Pro Ala Glu Pro Glu Gly Gly Pro Ala Ser Glu Gly Ala 
            420                 425                 430         


Ala Arg Pro Leu Pro Arg Phe Asn Ser Val Pro Leu Thr Asp Thr Gly 
        435                 440                 445             


His Glu Arg Gln Ser Glu Gly Ser Phe Ser Pro Gln Leu Gln Glu Ser 
    450                 455                 460                 


Val Phe His Leu Leu Val Pro Ser Val Ile Leu Val Leu Leu Ala Val 
465                 470                 475                 480 


Gly Gly Leu Leu Phe Tyr Arg Trp Arg Arg Arg Ser His Gln Glu Pro 
                485                 490                 495     


Gln Arg Ala Asp Ser Pro Leu Glu Gln Pro Glu Gly Ser Pro Leu Thr 
            500                 505                 510         


Gln Asp Asp Arg Gln Val Glu Leu Pro Val 
        515                 520         


