                         SEQUENCE LISTING

<110>  UNIVERSITY OF WASHINGTON and THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
 
<120>  COMPOSITIONS AND METHODS FOR TREATING CELIAC SPRUE DISEASE

<130>  20-1718-WO

<150>  US 63/108,163
<151>  2020-10-30

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  552
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma062-M

<400>  1

Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala Val 
1               5                   10                  15      


Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys Gly 
            20                  25                  30          


Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg Arg 
        35                  40                  45              


Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala Ile 
    50                  55                  60                  


Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala Ser 
65                  70                  75                  80  


His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala Asp 
                85                  90                  95      


Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr Ala 
            100                 105                 110         


Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val Glu 
        115                 120                 125             


Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu Gly 
    130                 135                 140                 


Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val Leu 
145                 150                 155                 160 


Gly Leu Asp Thr Arg Pro Val Ala Arg Arg Arg Phe Arg Met Gln Arg 
                165                 170                 175     


Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala Ala Ala Pro Thr 
            180                 185                 190         


Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln Phe Pro Glu Gly 
        195                 200                 205             


Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu Leu Gly Gly Gly 
    210                 215                 220                 


Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser Leu Gly Val Pro 
225                 230                 235                 240 


Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala Ser Asn Gln Pro 
                245                 250                 255     


Thr Gly Asp Pro Glu Gly Pro Asp Gly Glu Val Thr Leu Asp Ile Glu 
            260                 265                 270         


Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala Val Tyr Phe Ala 
        275                 280                 285             


Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr Thr Ala Ile His 
    290                 295                 300                 


Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser Trp Ser Met Pro 
305                 310                 315                 320 


Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met Asn Arg Ala Phe 
                325                 330                 335     


Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala Ala Ala Gly Asp 
            340                 345                 350         


Gln Gly Ser Thr Ser Gly Glu Gln Asp Gly Leu Tyr His Val His Phe 
        355                 360                 365             


Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly Thr Arg Leu Val 
    370                 375                 380                 


Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp Asn Gln Gly Pro 
385                 390                 395                 400 


Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile Phe Pro Leu Pro 
                405                 410                 415     


Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala Asn Pro Gly Ala 
            420                 425                 430         


Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn Ala Asp Pro Gln 
        435                 440                 445             


Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr Val Thr Gly Gly 
    450                 455                 460                 


Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val Ala Arg Ile Asn 
465                 470                 475                 480 


Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro Thr Leu Tyr Gln 
                485                 490                 495     


Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly Asn Asn Asp Ile 
            500                 505                 510         


Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly Trp Asp Pro Cys 
        515                 520                 525             


Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu Gln Ala Leu Leu 
    530                 535                 540                 


Pro Ser Ala Ser Gln Pro Gln Pro 
545                 550         


<210>  2
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma011 Pre-Protein Domain

<400>  2

Met Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala 
1               5                   10                  15      


Val Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys 
            20                  25                  30          


Gly Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg 
        35                  40                  45              


Arg Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala 
    50                  55                  60                  


Ile Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala 
65                  70                  75                  80  


Ser His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala 
                85                  90                  95      


Asp Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr 
            100                 105                 110         


Ala Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val 
        115                 120                 125             


Glu Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu 
    130                 135                 140                 


Gly Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val 
145                 150                 155                 160 


Leu Gly Leu Asp Thr Arg Pro Val Ala Arg Pro His Phe Arg Met Gln 
                165                 170                 175     


Arg Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala 
            180                 185                 


<210>  3
<211>  364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma011 Mature Peptide

<400>  3

Ala Ala Pro Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln 
1               5                   10                  15      


Phe Pro Glu Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu 
            20                  25                  30          


Leu Gly Gly Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser 
        35                  40                  45              


Leu Gly Val Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala 
    50                  55                  60                  


Ser Asn Gln Pro Thr Gly Asp Pro Lys Gly Pro Asp Gly Glu Val Glu 
65                  70                  75                  80  


Leu Asp Ile Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala 
                85                  90                  95      


Val Tyr Phe Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr 
            100                 105                 110         


Thr Ala Ile His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser 
        115                 120                 125             


Trp Ser Gly Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met 
    130                 135                 140                 


Asn Arg Ala Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala 
145                 150                 155                 160 


Ala Ala Gly Asp Ser Gly Ser Thr Gly Gly Glu Gln Asp Gly Leu Tyr 
                165                 170                 175     


His Val His Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly 
            180                 185                 190         


Thr Arg Leu Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp 
        195                 200                 205             


Asn Asp Gly Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile 
    210                 215                 220                 


Phe Pro Leu Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala 
225                 230                 235                 240 


Asn Pro Gly Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn 
                245                 250                 255     


Ala Asp Pro Ala Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr 
            260                 265                 270         


Val Ile Gly Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val 
        275                 280                 285             


Ala Arg Ile Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro 
    290                 295                 300                 


Thr Leu Tyr Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly 
305                 310                 315                 320 


Asn Asn Asp Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly 
                325                 330                 335     


Trp Asp Pro Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu 
            340                 345                 350         


Gln Ala Leu Leu Pro Ser Ala Ser Gln Pro Gln Pro 
        355                 360                 


<210>  4
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma010 (Full Length)

<400>  4

Met Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala 
1               5                   10                  15      


Val Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys 
            20                  25                  30          


Gly Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg 
        35                  40                  45              


Arg Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala 
    50                  55                  60                  


Ile Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala 
65                  70                  75                  80  


Ser His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala 
                85                  90                  95      


Asp Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr 
            100                 105                 110         


Ala Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val 
        115                 120                 125             


Glu Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu 
    130                 135                 140                 


Gly Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val 
145                 150                 155                 160 


Leu Gly Leu Asp Thr Arg Pro Val Ala Arg Pro His Phe Arg Met Gln 
                165                 170                 175     


Arg Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala Ala Ala Pro 
            180                 185                 190         


Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln Phe Pro Glu 
        195                 200                 205             


Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu Leu Gly Gly 
    210                 215                 220                 


Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser Leu Gly Val 
225                 230                 235                 240 


Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala Ser Asn Gln 
                245                 250                 255     


Pro Thr Gly Asp Pro Lys Gly Pro Asp Gly Glu Val Glu Leu Asp Ile 
            260                 265                 270         


Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala Val Tyr Phe 
        275                 280                 285             


Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr Thr Ala Ile 
    290                 295                 300                 


His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser Trp Ser Gly 
305                 310                 315                 320 


Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met Asn Arg Ala 
                325                 330                 335     


Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala Ala Ala Gly 
            340                 345                 350         


Asp Ser Gly Ser Thr Gly Gly Glu Gln Asp Gly Leu Tyr His Val His 
        355                 360                 365             


Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly Thr Arg Leu 
    370                 375                 380                 


Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp Asn Asp Gly 
385                 390                 395                 400 


Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile Phe Pro Leu 
                405                 410                 415     


Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala Asn Pro Gly 
            420                 425                 430         


Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn Ala Asp Pro 
        435                 440                 445             


Ala Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr Val Ile Gly 
    450                 455                 460                 


Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val Ala Arg Ile 
465                 470                 475                 480 


Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro Thr Leu Tyr 
                485                 490                 495     


Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly Asn Asn Asp 
            500                 505                 510         


Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly Trp Asp Pro 
        515                 520                 525             


Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu Gln Ala Leu 
    530                 535                 540                 


Leu Pro Ser Ala Ser Gln Pro Gln Pro Gly Ser Thr Glu Asn Leu Tyr 
545                 550                 555                 560 


Phe Gln Ser Gly Ala Leu Glu His His His His His His 
                565                 570             


<210>  5
<211>  384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma010 Mature Peptide

<400>  5

Ala Ala Pro Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln 
1               5                   10                  15      


Phe Pro Glu Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu 
            20                  25                  30          


Leu Gly Gly Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser 
        35                  40                  45              


Leu Gly Val Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala 
    50                  55                  60                  


Ser Asn Gln Pro Thr Gly Asp Pro Lys Gly Pro Asp Gly Glu Val Glu 
65                  70                  75                  80  


Leu Asp Ile Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala 
                85                  90                  95      


Val Tyr Phe Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr 
            100                 105                 110         


Thr Ala Ile His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser 
        115                 120                 125             


Trp Ser Gly Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met 
    130                 135                 140                 


Asn Arg Ala Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala 
145                 150                 155                 160 


Ala Ala Gly Asp Ser Gly Ser Thr Gly Gly Glu Gln Asp Gly Leu Tyr 
                165                 170                 175     


His Val His Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly 
            180                 185                 190         


Thr Arg Leu Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp 
        195                 200                 205             


Asn Asp Gly Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile 
    210                 215                 220                 


Phe Pro Leu Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala 
225                 230                 235                 240 


Asn Pro Gly Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn 
                245                 250                 255     


Ala Asp Pro Ala Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr 
            260                 265                 270         


Val Ile Gly Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val 
        275                 280                 285             


Ala Arg Ile Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro 
    290                 295                 300                 


Thr Leu Tyr Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly 
305                 310                 315                 320 


Asn Asn Asp Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly 
                325                 330                 335     


Trp Asp Pro Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu 
            340                 345                 350         


Gln Ala Leu Leu Pro Ser Ala Ser Gln Pro Gln Pro Gly Ser Thr Glu 
        355                 360                 365             


Asn Leu Tyr Phe Gln Ser Gly Ala Leu Glu His His His His His His 
    370                 375                 380                 


<210>  6
<211>  553
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma011

(Full Length)

<400>  6

Met Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala 
1               5                   10                  15      


Val Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys 
            20                  25                  30          


Gly Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg 
        35                  40                  45              


Arg Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala 
    50                  55                  60                  


Ile Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala 
65                  70                  75                  80  


Ser His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala 
                85                  90                  95      


Asp Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr 
            100                 105                 110         


Ala Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val 
        115                 120                 125             


Glu Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu 
    130                 135                 140                 


Gly Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val 
145                 150                 155                 160 


Leu Gly Leu Asp Thr Arg Pro Val Ala Arg Pro His Phe Arg Met Gln 
                165                 170                 175     


Arg Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala Ala Ala Pro 
            180                 185                 190         


Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln Phe Pro Glu 
        195                 200                 205             


Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu Leu Gly Gly 
    210                 215                 220                 


Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser Leu Gly Val 
225                 230                 235                 240 


Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala Ser Asn Gln 
                245                 250                 255     


Pro Thr Gly Asp Pro Lys Gly Pro Asp Gly Glu Val Glu Leu Asp Ile 
            260                 265                 270         


Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala Val Tyr Phe 
        275                 280                 285             


Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr Thr Ala Ile 
    290                 295                 300                 


His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser Trp Ser Gly 
305                 310                 315                 320 


Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met Asn Arg Ala 
                325                 330                 335     


Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala Ala Ala Gly 
            340                 345                 350         


Asp Ser Gly Ser Thr Gly Gly Glu Gln Asp Gly Leu Tyr His Val His 
        355                 360                 365             


Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly Thr Arg Leu 
    370                 375                 380                 


Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp Asn Asp Gly 
385                 390                 395                 400 


Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile Phe Pro Leu 
                405                 410                 415     


Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala Asn Pro Gly 
            420                 425                 430         


Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn Ala Asp Pro 
        435                 440                 445             


Ala Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr Val Ile Gly 
    450                 455                 460                 


Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val Ala Arg Ile 
465                 470                 475                 480 


Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro Thr Leu Tyr 
                485                 490                 495     


Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly Asn Asn Asp 
            500                 505                 510         


Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly Trp Asp Pro 
        515                 520                 525             


Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu Gln Ala Leu 
    530                 535                 540                 


Leu Pro Ser Ala Ser Gln Pro Gln Pro 
545                 550             


<210>  7
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma062-M Pre-Protein Domain

<400>  7

Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala Val 
1               5                   10                  15      


Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys Gly 
            20                  25                  30          


Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg Arg 
        35                  40                  45              


Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala Ile 
    50                  55                  60                  


Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala Ser 
65                  70                  75                  80  


His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala Asp 
                85                  90                  95      


Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr Ala 
            100                 105                 110         


Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val Glu 
        115                 120                 125             


Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu Gly 
    130                 135                 140                 


Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val Leu 
145                 150                 155                 160 


Gly Leu Asp Thr Arg Pro Val Ala Arg Arg Arg Phe Arg Met Gln Arg 
                165                 170                 175     


Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala 
            180                 185             


<210>  8
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kuma062-M Mature Peptide

<400>  8

Ala Ala Pro Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln 
1               5                   10                  15      


Phe Pro Glu Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu 
            20                  25                  30          


Leu Gly Gly Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser 
        35                  40                  45              


Leu Gly Val Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala 
    50                  55                  60                  


Ser Asn Gln Pro Thr Gly Asp Pro Glu Gly Pro Asp Gly Glu Val Thr 
65                  70                  75                  80  


Leu Asp Ile Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala 
                85                  90                  95      


Val Tyr Phe Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr 
            100                 105                 110         


Thr Ala Ile His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser 
        115                 120                 125             


Trp Ser Met Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met 
    130                 135                 140                 


Asn Arg Ala Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala 
145                 150                 155                 160 


Ala Ala Gly Asp Gln Gly Ser Thr Ser Gly Glu Gln Asp Gly Leu Tyr 
                165                 170                 175     


His Val His Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly 
            180                 185                 190         


Thr Arg Leu Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp 
        195                 200                 205             


Asn Gln Gly Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile 
    210                 215                 220                 


Phe Pro Leu Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala 
225                 230                 235                 240 


Asn Pro Gly Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn 
                245                 250                 255     


Ala Asp Pro Gln Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr 
            260                 265                 270         


Val Thr Gly Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val 
        275                 280                 285             


Ala Arg Ile Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro 
    290                 295                 300                 


Thr Leu Tyr Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly 
305                 310                 315                 320 


Asn Asn Asp Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly 
                325                 330                 335     


Trp Asp Pro Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu 
            340                 345                 350         


Gln Ala Leu Leu Pro Ser Ala Ser Gln Pro Gln 
        355                 360             


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gliadin

<400>  9

Pro Gln Leu Pro 
1               


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gliadin

<400>  10

Pro Gln Gln Pro 
1               


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gliadin

<400>  11

Pro Phe Pro Gln Pro Gln Leu Pro Tyr 
1               5                   


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gliadin

<400>  12

Pro Phe Pro Gln Pro Gln Gln Pro Phe 
1               5                   


<210>  13
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide

<400>  13

Leu Gln Leu Gln Pro Phe Pro Gln Pro Gln Leu Pro Tyr Pro Gln Pro 
1               5                   10                  15      


Gln Leu Pro Tyr Pro Gln Pro Gln Leu Pro Tyr Pro Gln Pro Gln Pro 
            20                  25                  30          


Phe 
    


<210>  14
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide

<400>  14

Phe Leu Gln Pro Gln Gln Pro Phe Pro Gln Gln Pro Gln Gln Pro Tyr 
1               5                   10                  15      


Pro Gln Gln Pro Gln Gln Pro Phe Pro Gln 
            20                  25      


<210>  15
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavable histidine tag


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Xaa is  Lys or Gln

<220>
<221>  misc_feature
<222>  (30)..(55)
<223>  Xaa is any amino acid

<400>  15

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Pro Gln Xaa Pro Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa His His His His His His 
    50                  55                  60  


<210>  16
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavable histidine tag

<400>  16

Gly Ser Ser Gly Ser Ser Gly Ser Gln Pro Gln Leu Pro Tyr Gly Ser 
1               5                   10                  15      


Ser Gly Ser Ser Gly Ser His His His His His His 
            20                  25              


<210>  17
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  histidine tag

<400>  17

Gly Ser Thr Glu Asn Leu Tyr Phe Gln Ser Gly Ala Leu Glu His His 
1               5                   10                  15      


His His His His 
            20  


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV Protease Cut site

<400>  18

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R5

<400>  19

Gln Gln Pro Phe Pro 
1               5   


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G12

<400>  20

Gln Pro Gln Leu Pro Tyr 
1               5       


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gliadinase

<400>  21

Ser Asp Met Glu 
1               


<210>  22
<211>  1659
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A nucleic acid that encodes the Kuma062-M Protein

<400>  22
agtgatatgg aaaaaccgtg gaaagaaggt gaagaagccc gcgcagtgct gcaaggtcat       60

gctcgtgcgc aggcaccgca agcagtcgat aaaggcccgg tggcaggtga cgaacgcatg      120

gctgttaccg tggttctgcg tcgccagcgt gcaggtgaac tggcggccca cgtggaacgt      180

caagcagcta ttgctccgca tgcgcgcgaa cacctgaaac gtgaagcgtt tgcggccagt      240

catggtgcgt ccctggatga ctttgccgaa ctgcgtcgct tcgcagatgc tcacggcctg      300

gcgctggacc gtgcaaacgt tgcagctggc accgccgttc tgtctggtcc ggacgatgca      360

atcaatcgcg cttttggtgt ggaactgcgt catttcgatc acccggacgg ctcatatcgt      420

tcgtacctgg gtgaagtcac cgtgccggcc agtattgcac cgatgatcga agcggttctg      480

ggcctggata cgcgtccggt cgcccgccgt cgttttcgta tgcagcgtcg cgcagaaggc      540

ggtttcgaag ctcgttccca agcggcggca ccgaccgcat atacgccgct ggatgttgcg      600

caggcctacc aatttccgga aggtctggac ggccagggtc aatgcattgc cattatcgaa      660

ctgggcggtg gctatgatga agcttcactg gcgcagtact tcgcgtcgct gggcgtgccg      720

gcaccgcaag tcgtgagtgt ttccgtcgat ggtgcgagca accagccgac cggtgatccg      780

gaaggtccgg acggtgaagt gaccctggat atcgaagttg caggcgctct ggcgccgggt      840

gccaaatttg cagtgtattt cgcgccggat accactgccg gttttctgga cgcgattacc      900

acggccatcc acgatccgac gctgaaaccg agcgttgtct caatttcgtg gagcatgccg      960

gaagacagct ggacctctgc tgcgatcgcc gcaatgaacc gtgcgtttct ggatgctgcg     1020

gccctgggtg tgaccgttct ggcagctgcg ggcgaccagg gttctacgag cggcgaacag     1080

gacggtctgt atcatgtgca tttcccggcc gcatcaccgt acgttctggc gtgcggtggc     1140

acgcgcctgg tcgcatcggg tggccgtatt gcgcaggaaa ccgtctggaa ccagggtccg     1200

gacggtggtg caacgggtgg cggtgtgagc cgcatcttcc cgctgccggc atggcaggaa     1260

cacgctaacg ttccgccgtc tgcaaatccg ggcgcgagca gcggccgtgg tgtcccggat     1320

ctggctggta atgcggaccc gcagaccggt tatgaagtgg ttattgatgg cgaagcaacc     1380

gtcaccggcg gtacgagcgc cgtggcaccg ctgtttgctg cgctggttgc gcgtattaac     1440

cagaaactgg gcaaagcagt tggttatctg aatccgaccc tgtaccaact gccggcagat     1500

gttttccatg acatcacgga gggtaacaat gatattgcaa accgtgcgca gatttatcaa     1560

gcaggtccgg gctgggaccc gtgtaccggt ctgggttcac cgattggtgt gcgtctgctg     1620

caagcactgt tgccgagtgc ctcccagccg caaccgtga                            1659


<210>  23
<211>  553
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Wherein Xaa is not Met.

<400>  23

Xaa Ser Asp Met Glu Lys Pro Trp Lys Glu Gly Glu Glu Ala Arg Ala 
1               5                   10                  15      


Val Leu Gln Gly His Ala Arg Ala Gln Ala Pro Gln Ala Val Asp Lys 
            20                  25                  30          


Gly Pro Val Ala Gly Asp Glu Arg Met Ala Val Thr Val Val Leu Arg 
        35                  40                  45              


Arg Gln Arg Ala Gly Glu Leu Ala Ala His Val Glu Arg Gln Ala Ala 
    50                  55                  60                  


Ile Ala Pro His Ala Arg Glu His Leu Lys Arg Glu Ala Phe Ala Ala 
65                  70                  75                  80  


Ser His Gly Ala Ser Leu Asp Asp Phe Ala Glu Leu Arg Arg Phe Ala 
                85                  90                  95      


Asp Ala His Gly Leu Ala Leu Asp Arg Ala Asn Val Ala Ala Gly Thr 
            100                 105                 110         


Ala Val Leu Ser Gly Pro Asp Asp Ala Ile Asn Arg Ala Phe Gly Val 
        115                 120                 125             


Glu Leu Arg His Phe Asp His Pro Asp Gly Ser Tyr Arg Ser Tyr Leu 
    130                 135                 140                 


Gly Glu Val Thr Val Pro Ala Ser Ile Ala Pro Met Ile Glu Ala Val 
145                 150                 155                 160 


Leu Gly Leu Asp Thr Arg Pro Val Ala Arg Arg Arg Phe Arg Met Gln 
                165                 170                 175     


Arg Arg Ala Glu Gly Gly Phe Glu Ala Arg Ser Gln Ala Ala Ala Pro 
            180                 185                 190         


Thr Ala Tyr Thr Pro Leu Asp Val Ala Gln Ala Tyr Gln Phe Pro Glu 
        195                 200                 205             


Gly Leu Asp Gly Gln Gly Gln Cys Ile Ala Ile Ile Glu Leu Gly Gly 
    210                 215                 220                 


Gly Tyr Asp Glu Ala Ser Leu Ala Gln Tyr Phe Ala Ser Leu Gly Val 
225                 230                 235                 240 


Pro Ala Pro Gln Val Val Ser Val Ser Val Asp Gly Ala Ser Asn Gln 
                245                 250                 255     


Pro Thr Gly Asp Pro Glu Gly Pro Asp Gly Glu Val Thr Leu Asp Ile 
            260                 265                 270         


Glu Val Ala Gly Ala Leu Ala Pro Gly Ala Lys Phe Ala Val Tyr Phe 
        275                 280                 285             


Ala Pro Asp Thr Thr Ala Gly Phe Leu Asp Ala Ile Thr Thr Ala Ile 
    290                 295                 300                 


His Asp Pro Thr Leu Lys Pro Ser Val Val Ser Ile Ser Trp Ser Met 
305                 310                 315                 320 


Pro Glu Asp Ser Trp Thr Ser Ala Ala Ile Ala Ala Met Asn Arg Ala 
                325                 330                 335     


Phe Leu Asp Ala Ala Ala Leu Gly Val Thr Val Leu Ala Ala Ala Gly 
            340                 345                 350         


Asp Gln Gly Ser Thr Ser Gly Glu Gln Asp Gly Leu Tyr His Val His 
        355                 360                 365             


Phe Pro Ala Ala Ser Pro Tyr Val Leu Ala Cys Gly Gly Thr Arg Leu 
    370                 375                 380                 


Val Ala Ser Gly Gly Arg Ile Ala Gln Glu Thr Val Trp Asn Gln Gly 
385                 390                 395                 400 


Pro Asp Gly Gly Ala Thr Gly Gly Gly Val Ser Arg Ile Phe Pro Leu 
                405                 410                 415     


Pro Ala Trp Gln Glu His Ala Asn Val Pro Pro Ser Ala Asn Pro Gly 
            420                 425                 430         


Ala Ser Ser Gly Arg Gly Val Pro Asp Leu Ala Gly Asn Ala Asp Pro 
        435                 440                 445             


Gln Thr Gly Tyr Glu Val Val Ile Asp Gly Glu Ala Thr Val Thr Gly 
    450                 455                 460                 


Gly Thr Ser Ala Val Ala Pro Leu Phe Ala Ala Leu Val Ala Arg Ile 
465                 470                 475                 480 


Asn Gln Lys Leu Gly Lys Ala Val Gly Tyr Leu Asn Pro Thr Leu Tyr 
                485                 490                 495     


Gln Leu Pro Ala Asp Val Phe His Asp Ile Thr Glu Gly Asn Asn Asp 
            500                 505                 510         


Ile Ala Asn Arg Ala Gln Ile Tyr Gln Ala Gly Pro Gly Trp Asp Pro 
        515                 520                 525             


Cys Thr Gly Leu Gly Ser Pro Ile Gly Val Arg Leu Leu Gln Ala Leu 
    530                 535                 540                 


Leu Pro Ser Ala Ser Gln Pro Gln Pro 
545                 550             


