                         SEQUENCE LISTING

<110>  Biosion Inc.
 
<120>  NANOBODIES BINDING TROP2 AND USES THEREOF

<130>  55532 00042

<150>  CN202011209105.X
<151>  2020-11-03

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH CDR1 for 01-9F, 01-9F-CDR-V1 to 01-9F-CDR-V11, 
       01-9F-CDR-V11-V1 to 01-9F-CDR-V11-V24

<400>  1

Arg Tyr Cys Val Ala 
1               5   


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH CDR2 for 01-9F and 01-5A antibodies


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Asp or Glu

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be Gly or Ala

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Xaa can be Asp or Glu

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Xaa can be Ser or Thr

<400>  2

Arg Ile Leu Ser Xaa Xaa Thr Thr Ser Tyr Ser Xaa Xaa Val Lys Gly 
1               5                   10                  15      


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH CDR3 for 01-9F and 01-5A antibodies


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be Asp, Glu or Ile

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be Gly or Ala

<400>  3

Glu Ala Phe Arg Pro Phe Thr Pro Ser Xaa Xaa Asp Cys Thr Thr Val 
1               5                   10                  15      


Leu Gly Ile Asp Tyr 
            20      


<210>  4
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F, 01-9F-CDR-V1 to 01-9F-CDR-V3, and 01-5A


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be Ser or Thr

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  Xaa can be Gln or Gly

<220>
<221>  misc_feature
<222>  (107)..(107)
<223>  Xaa can be Asp, Glu or Ile

<220>
<221>  misc_feature
<222>  (108)..(108)
<223>  Xaa can be Gly or Ala

<220>
<221>  misc_feature
<222>  (124)..(124)
<223>  Xaa can be Pro or Leu

<400>  4

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Xaa Val Xaa Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Val Ser Gly Leu Pro Tyr Glu Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Gly Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Gly Thr Thr Ser Tyr Ser Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Glu Ala Phe Arg Pro Phe Thr Pro Ser Xaa Xaa Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Xaa Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  5
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V4, 01-9F-CDR-V5, 01-9F-CDR-V8 and 01-9F-CDR-V9


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  Xaa can be Glu or Asp

<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  Xaa can be Gly or Ala

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  Xaa can be Asp or Glu

<400>  5

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Val Ser Gly Leu Pro Tyr Glu Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Gly Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Xaa Xaa Thr Thr Ser Tyr Ser Xaa Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Glu Ala Phe Arg Pro Phe Thr Pro Ser Glu Gly Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Pro Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  6
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V6, 01-9F-CDR-V7, 01-9F-CDR-V10 and 
       01-9F-CDR-V11


<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  Xaa can be Asp or Glu

<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  Xaa can be Gly or Ala
<220>
<221>  misc_feature
<222>  (62)..(62)
<223>  Xaa can be Ser or Thr

<400>  6

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Val Ser Gly Leu Pro Tyr Glu Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Gly Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Xaa Xaa Thr Thr Ser Tyr Ser Asp Xaa Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Pro Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  7
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V1

<400>  7

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Leu Pro Tyr Glu Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Lys Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  8
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V2 to 01-9F-CDR-V11-V6


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be Phe or Leu

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be Tyr or Phe

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be Lys or Arg

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be Ala or Arg

<400>  8

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Xaa Thr Xaa Ser Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Xaa Asp Asn Ala Lys Asn Ser Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Xaa Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  9
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V7 to 01-9F-CDR-V11-V11


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be Phe or Leu

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be Tyr or Phe

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be Lys or Arg

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be Ala or Arg

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Xaa Pro Xaa Ser Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Xaa Asp Asn Ala Lys Asn Ser Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Xaa Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  10
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V12 to 01-9F-CDR-V11-V16


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be Phe or Leu

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be Tyr or Phe

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be Lys or Arg

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be Ala or Arg

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Xaa Thr Xaa Glu Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Xaa Asp Asn Ala Lys Asn Ser Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Xaa Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  11
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V17 to 01-9F-CDR-V11-V21


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be Phe or Leu

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be Tyr or Phe

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be Lys or Arg

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Xaa can be Ala or Arg

<400>  11

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Xaa Thr Xaa Ser Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Gly Leu Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Xaa Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Xaa Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  12
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V22

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Gly Ser Glu Tyr Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Phe Arg Gln Ala Pro Gly Gln Gly Leu Glu Ala Val 
        35                  40                  45              


Ala Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  13
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH of 01-9F-CDR-V11-V23 and 01-9F-CDR-V11-V24


<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  Xaa can be Val or Phe

<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  Xaa can be Trp or Gly

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Arg Tyr 
            20                  25                  30          


Cys Val Ala Trp Xaa Arg Gln Ala Pro Gly Lys Gly Leu Glu Xaa Val 
        35                  40                  45              


Ser Arg Ile Leu Ser Asp Ala Thr Thr Ser Tyr Ser Asp Thr Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Glu Ala Phe Arg Pro Phe Thr Pro Ser Asp Ala Asp Cys Thr Thr 
            100                 105                 110         


Val Leu Gly Ile Asp Tyr Trp Gly Lys Gly Thr Thr Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  14
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Constant region for heavy chain only antibodies

<400>  14

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  15
<211>  502
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human TROP2-Fc protein

<400>  15

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Gly Ser Arg Cys His Thr Ala Ala Gln Asp Asn Cys Thr Cys 
            20                  25                  30          


Pro Thr Asn Lys Met Thr Val Cys Ser Pro Asp Gly Pro Gly Gly Arg 
        35                  40                  45              


Cys Gln Cys Arg Ala Leu Gly Ser Gly Met Ala Val Asp Cys Ser Thr 
    50                  55                  60                  


Leu Thr Ser Lys Cys Leu Leu Leu Lys Ala Arg Met Ser Ala Pro Lys 
65                  70                  75                  80  


Asn Ala Arg Thr Leu Val Arg Pro Ser Glu His Ala Leu Val Asp Asn 
                85                  90                  95      


Asp Gly Leu Tyr Asp Pro Asp Cys Asp Pro Glu Gly Arg Phe Lys Ala 
            100                 105                 110         


Arg Gln Cys Asn Gln Thr Ser Val Cys Trp Cys Val Asn Ser Val Gly 
        115                 120                 125             


Val Arg Arg Thr Asp Lys Gly Asp Leu Ser Leu Arg Cys Asp Glu Leu 
    130                 135                 140                 


Val Arg Thr His His Ile Leu Ile Asp Leu Arg His Arg Pro Thr Ala 
145                 150                 155                 160 


Gly Ala Phe Asn His Ser Asp Leu Asp Ala Glu Leu Arg Arg Leu Phe 
                165                 170                 175     


Arg Glu Arg Tyr Arg Leu His Pro Lys Phe Val Ala Ala Val His Tyr 
            180                 185                 190         


Glu Gln Pro Thr Ile Gln Ile Glu Leu Arg Gln Asn Thr Ser Gln Lys 
        195                 200                 205             


Ala Ala Gly Asp Val Asp Ile Gly Asp Ala Ala Tyr Tyr Phe Glu Arg 
    210                 215                 220                 


Asp Ile Lys Gly Glu Ser Leu Phe Gln Gly Arg Gly Gly Leu Asp Leu 
225                 230                 235                 240 


Arg Val Arg Gly Glu Pro Leu Gln Val Glu Arg Thr Leu Ile Tyr Tyr 
                245                 250                 255     


Leu Asp Glu Ile Pro Pro Lys Phe Ser Met Lys Arg Leu Thr Glu Pro 
            260                 265                 270         


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
        275                 280                 285             


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
    290                 295                 300                 


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
305                 310                 315                 320 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
                325                 330                 335     


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
            340                 345                 350         


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
        355                 360                 365             


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
    370                 375                 380                 


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
385                 390                 395                 400 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
                405                 410                 415     


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
            420                 425                 430         


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
        435                 440                 445             


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
    450                 455                 460                 


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
465                 470                 475                 480 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
                485                 490                 495     


Ser Leu Ser Pro Gly Lys 
            500         


<210>  16
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human TROP2-his protein

<400>  16

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Phe Pro Gly Ser Arg Cys His Thr Ala Ala Gln Asp Asn Cys Thr Cys 
            20                  25                  30          


Pro Thr Asn Lys Met Thr Val Cys Ser Pro Asp Gly Pro Gly Gly Arg 
        35                  40                  45              


Cys Gln Cys Arg Ala Leu Gly Ser Gly Met Ala Val Asp Cys Ser Thr 
    50                  55                  60                  


Leu Thr Ser Lys Cys Leu Leu Leu Lys Ala Arg Met Ser Ala Pro Lys 
65                  70                  75                  80  


Asn Ala Arg Thr Leu Val Arg Pro Ser Glu His Ala Leu Val Asp Asn 
                85                  90                  95      


Asp Gly Leu Tyr Asp Pro Asp Cys Asp Pro Glu Gly Arg Phe Lys Ala 
            100                 105                 110         


Arg Gln Cys Asn Gln Thr Ser Val Cys Trp Cys Val Asn Ser Val Gly 
        115                 120                 125             


Val Arg Arg Thr Asp Lys Gly Asp Leu Ser Leu Arg Cys Asp Glu Leu 
    130                 135                 140                 


Val Arg Thr His His Ile Leu Ile Asp Leu Arg His Arg Pro Thr Ala 
145                 150                 155                 160 


Gly Ala Phe Asn His Ser Asp Leu Asp Ala Glu Leu Arg Arg Leu Phe 
                165                 170                 175     


Arg Glu Arg Tyr Arg Leu His Pro Lys Phe Val Ala Ala Val His Tyr 
            180                 185                 190         


Glu Gln Pro Thr Ile Gln Ile Glu Leu Arg Gln Asn Thr Ser Gln Lys 
        195                 200                 205             


Ala Ala Gly Asp Val Asp Ile Gly Asp Ala Ala Tyr Tyr Phe Glu Arg 
    210                 215                 220                 


Asp Ile Lys Gly Glu Ser Leu Phe Gln Gly Arg Gly Gly Leu Asp Leu 
225                 230                 235                 240 


Arg Val Arg Gly Glu Pro Leu Gln Val Glu Arg Thr Leu Ile Tyr Tyr 
                245                 250                 255     


Leu Asp Glu Ile Pro Pro Lys Phe Ser Met Lys Arg Leu Thr His His 
            260                 265                 270         


His His His His His His His His 
        275                 280 


<210>  17
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of sacituzumab

<400>  17

Ser Val Gln Leu Gln Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Thr Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Ala Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Ser Ser Leu Lys Ala Asp Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Gly Phe Gly Ser Ser Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Gln Gly Ser Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys 
    210                 215                 220                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
            260                 265                 270         


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Pro Gly Lys 
    450     


<210>  18
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain of sacituzumab

<400>  18

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  19
<211>  282
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cynomolgus monkey TROP2-his protein

<400>  19

Met Ala Arg Gly Pro Gly Leu Ala Pro Pro Pro Leu Arg Leu Pro Leu 
1               5                   10                  15      


Leu Leu Leu Leu Leu Ala Ala Val Thr Gly His Thr Ala Ala Gln Asp 
            20                  25                  30          


Asn Cys Thr Cys Pro Thr Asn Lys Met Thr Val Cys Ser Pro Asp Gly 
        35                  40                  45              


Pro Gly Gly Arg Cys Gln Cys Arg Ala Leu Gly Ser Gly Val Ala Val 
    50                  55                  60                  


Asp Cys Ser Thr Leu Thr Ser Lys Cys Leu Leu Leu Lys Ala Arg Met 
65                  70                  75                  80  


Ser Ala Pro Lys Asn Ala Arg Thr Leu Val Arg Pro Asn Glu His Ala 
                85                  90                  95      


Leu Val Asp Asn Asp Gly Leu Tyr Asp Pro Asp Cys Asp Pro Glu Gly 
            100                 105                 110         


Arg Phe Lys Ala Arg Gln Cys Asn Gln Thr Ser Val Cys Trp Cys Val 
        115                 120                 125             


Asn Ser Val Gly Val Arg Arg Thr Asp Lys Gly Asp Leu Ser Leu Arg 
    130                 135                 140                 


Cys Asp Glu Leu Val Arg Thr His His Ile Leu Ile Asp Leu Arg His 
145                 150                 155                 160 


Arg Pro Thr Ala Gly Ala Phe Asn His Ser Asp Leu Asp Ala Glu Leu 
                165                 170                 175     


Arg Arg Leu Phe Arg Glu Arg Tyr Arg Leu His Pro Lys Phe Val Ala 
            180                 185                 190         


Ala Val His Tyr Glu Gln Pro Thr Ile Gln Ile Glu Leu Arg Gln Asn 
        195                 200                 205             


Thr Ser Gln Lys Ala Ala Gly Asp Val Asp Ile Gly Asp Ala Ala Tyr 
    210                 215                 220                 


Tyr Phe Glu Arg Asp Val Lys Gly Glu Ser Leu Phe Gln Gly Arg Gly 
225                 230                 235                 240 


Gly Leu Asp Leu Arg Val Arg Gly Glu Pro Leu Gln Val Glu Arg Thr 
                245                 250                 255     


Leu Ile Tyr Tyr Leu Asp Glu Ile Pro Pro Lys Phe Ser Met Lys Arg 
            260                 265                 270         


His His His His His His His His His His 
        275                 280         


<210>  20
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full length human TROP2

<400>  20

Met Ala Arg Gly Pro Gly Leu Ala Pro Pro Pro Leu Arg Leu Pro Leu 
1               5                   10                  15      


Leu Leu Leu Val Leu Ala Ala Val Thr Gly His Thr Ala Ala Gln Asp 
            20                  25                  30          


Asn Cys Thr Cys Pro Thr Asn Lys Met Thr Val Cys Ser Pro Asp Gly 
        35                  40                  45              


Pro Gly Gly Arg Cys Gln Cys Arg Ala Leu Gly Ser Gly Met Ala Val 
    50                  55                  60                  


Asp Cys Ser Thr Leu Thr Ser Lys Cys Leu Leu Leu Lys Ala Arg Met 
65                  70                  75                  80  


Ser Ala Pro Lys Asn Ala Arg Thr Leu Val Arg Pro Ser Glu His Ala 
                85                  90                  95      


Leu Val Asp Asn Asp Gly Leu Tyr Asp Pro Asp Cys Asp Pro Glu Gly 
            100                 105                 110         


Arg Phe Lys Ala Arg Gln Cys Asn Gln Thr Ser Val Cys Trp Cys Val 
        115                 120                 125             


Asn Ser Val Gly Val Arg Arg Thr Asp Lys Gly Asp Leu Ser Leu Arg 
    130                 135                 140                 


Cys Asp Glu Leu Val Arg Thr His His Ile Leu Ile Asp Leu Arg His 
145                 150                 155                 160 


Arg Pro Thr Ala Gly Ala Phe Asn His Ser Asp Leu Asp Ala Glu Leu 
                165                 170                 175     


Arg Arg Leu Phe Arg Glu Arg Tyr Arg Leu His Pro Lys Phe Val Ala 
            180                 185                 190         


Ala Val His Tyr Glu Gln Pro Thr Ile Gln Ile Glu Leu Arg Gln Asn 
        195                 200                 205             


Thr Ser Gln Lys Ala Ala Gly Asp Val Asp Ile Gly Asp Ala Ala Tyr 
    210                 215                 220                 


Tyr Phe Glu Arg Asp Ile Lys Gly Glu Ser Leu Phe Gln Gly Arg Gly 
225                 230                 235                 240 


Gly Leu Asp Leu Arg Val Arg Gly Glu Pro Leu Gln Val Glu Arg Thr 
                245                 250                 255     


Leu Ile Tyr Tyr Leu Asp Glu Ile Pro Pro Lys Phe Ser Met Lys Arg 
            260                 265                 270         


Leu Thr Ala Gly Leu Ile Ala Val Ile Val Val Val Val Val Ala Leu 
        275                 280                 285             


Val Ala Gly Met Ala Val Leu Val Ile Thr Asn Arg Arg Lys Ser Gly 
    290                 295                 300                 


Lys Tyr Lys Lys Val Glu Ile Lys Glu Leu Gly Glu Leu Arg Lys Glu 
305                 310                 315                 320 


Pro Ser Leu Gly Gly Gly Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
                325                 330                 335     


<210>  21
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DTTP-1170 protein

<400>  21

Met Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Lys His Gln Val Gln Leu Val Glu Ser Gly Gly Gly Trp Val Gln 
385                 390                 395                 400 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                405                 410                 415     


Ser Asp Thr Ala Met Met Trp Val Arg Gln Ala Pro Gly Lys Gly Arg 
            420                 425                 430         


Glu Trp Val Ala Ala Ile Asp Thr Gly Gly Gly Tyr Thr Tyr Tyr Ala 
        435                 440                 445             


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
    450                 455                 460                 


Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Arg 
465                 470                 475                 480 


Tyr Tyr Cys Ala Lys Thr Tyr Ser Gly Asn Tyr Tyr Ser Asn Tyr Thr 
                485                 490                 495     


Val Ala Asn Tyr Gly Thr Thr Gly Arg Gly Thr Leu Val Thr Val Ser 
            500                 505                 510         


Ser His His His His His His 
        515                 


<210>  22
<211>  624
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DT3C protein

<400>  22

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Met Gly Ala Asp Asp Val Val Asp Ser Ser 
            20                  25                  30          


Lys Ser Phe Val Met Glu Asn Phe Ser Ser Tyr His Gly Thr Lys Pro 
        35                  40                  45              


Gly Tyr Val Asp Ser Ile Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly 
    50                  55                  60                  


Thr Gln Gly Asn Tyr Asp Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp 
65                  70                  75                  80  


Asn Lys Tyr Asp Ala Ala Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu 
                85                  90                  95      


Ser Gly Lys Ala Gly Gly Val Val Lys Val Thr Tyr Pro Gly Leu Thr 
            100                 105                 110         


Lys Val Leu Ala Leu Lys Val Asp Asn Ala Glu Thr Ile Lys Lys Glu 
        115                 120                 125             


Leu Gly Leu Ser Leu Thr Glu Pro Leu Met Glu Gln Val Gly Thr Glu 
    130                 135                 140                 


Glu Phe Ile Lys Arg Phe Gly Asp Gly Ala Ser Arg Val Val Leu Ser 
145                 150                 155                 160 


Leu Pro Phe Ala Glu Gly Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp 
                165                 170                 175     


Glu Gln Ala Lys Ala Leu Ser Val Glu Leu Glu Ile Asn Phe Glu Thr 
            180                 185                 190         


Arg Gly Lys Arg Gly Gln Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala 
        195                 200                 205             


Cys Ala Gly Asn Arg Val Arg Arg Ser Val Gly Ser Ser Leu Ser Cys 
    210                 215                 220                 


Ile Asn Leu Asp Trp Asp Val Ile Arg Asp Lys Thr Lys Thr Lys Ile 
225                 230                 235                 240 


Glu Ser Leu Lys Glu His Gly Pro Ile Lys Asn Lys Met Ser Glu Ser 
                245                 250                 255     


Pro Asn Lys Thr Val Ser Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu 
            260                 265                 270         


Phe His Gln Thr Ala Leu Glu His Pro Glu Leu Ser Glu Leu Lys Thr 
        275                 280                 285             


Val Thr Gly Thr Asn Pro Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp 
    290                 295                 300                 


Ala Val Asn Val Ala Gln Val Ile Asp Ser Glu Thr Ala Asp Asn Leu 
305                 310                 315                 320 


Glu Lys Thr Thr Ala Ala Leu Ser Ile Leu Pro Gly Ile Gly Ser Val 
                325                 330                 335     


Met Gly Ile Ala Asp Gly Ala Val His His Asn Thr Glu Glu Ile Val 
            340                 345                 350         


Ala Gln Ser Ile Ala Leu Ser Ser Leu Met Val Ala Gln Ala Ile Pro 
        355                 360                 365             


Leu Val Gly Glu Leu Val Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val 
    370                 375                 380                 


Glu Ser Ile Ile Asn Leu Phe Gln Val Val His Asn Ser Tyr Asn Arg 
385                 390                 395                 400 


Pro Ala Tyr Ser Pro Gly His Lys His Ile Asp Glu Ile Leu Ala Ala 
                405                 410                 415     


Leu Pro Lys Thr Asp Thr Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu 
            420                 425                 430         


Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys 
        435                 440                 445             


Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr 
    450                 455                 460                 


Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu Val 
465                 470                 475                 480 


Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu Val 
                485                 490                 495     


Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val Asp 
            500                 505                 510         


Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly 
        515                 520                 525             


Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val 
    530                 535                 540                 


Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu Thr Pro Ala Val 
545                 550                 555                 560 


Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr 
                565                 570                 575     


Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln 
            580                 585                 590         


Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala 
        595                 600                 605             


Thr Lys Thr Phe Thr Val Thr Glu Leu Glu His His His His His His 
    610                 615                 620                 


<210>  23
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VHH for 01-9F

<400>  23
caagtgcaac ttgttgaaag cgggggcggt agcgtacagg cgggagggag cctccgattg       60

agctgcgtgg tcagcgggct gccgtatgag agatactgcg tagcatggtt caggcaaggc      120

ccgggtaaag agcgagaggg agtagctcgg atactttctg acggtactac gtcttatagt      180

gactccgtga aggggcgctt cactattagc aaggataatg cgaaaaacac attgtacctt      240

cagatgaaca gcctgaagag tgaggatacg gctacttatt attgtgcagc ggaagcattc      300

cgcccattca caccctccga cggggattgt accacagtgc ttggtataga ctactgggga      360

aaaggaacgc ctgttactgt gagcagc                                          387


<210>  24
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VHH for 01-9F-CDR-V11

<400>  24
caagtgcaac tggtagaatc tggggggggc agtgtacaag ctgggggcag cctgagactg       60

agctgtgtgg tgtctggcct gccctatgag agatactgtg tggcctggtt cagacaaggc      120

cctggcaagg agagagaggg ggtggctaga atcctgtctg atgccaccac aagctactct      180

gacacagtga agggcagatt caccatcagc aaggacaatg ccaagaacac cctgtacctg      240

cagatgaaca gcctgaagtc tgaggacaca gccacctact actgtgctgc tgaggccttc      300

agacccttca ccccctctga tgctgactgc accacagtgc tgggcattga ctactggggc      360

aagggcaccc ctgtgacagt gagctct                                          387


<210>  25
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VHH for 01-9F-CDR-V11-V11

<400>  25
gaggtgcagc tcgtggagag cggcgggggc ctggtgcaac ctggcgggag cctgagactg       60

agctgcgccg ctagcggcct gccctacagc agatactgcg tggcctggtt cagacaagcc      120

cccggcaagg gcctggaggg cgtggctaga atcctgagcg acgccaccac aagctacagc      180

gacaccgtga agggcagatt caccatcagc aaggacaacg ccaagaacag cctgtacctg      240

cagatgaaca gcctgagagc cgaggacacc gccgtgtact actgcgccgc cgaggccttc      300

agacccttca cccctagcga cgccgactgc accaccgtgc tgggcatcga ctactggggc      360

caaggcacca ccgtgaccgt gagcagc                                          387


<210>  26
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Constant region for heavy chain only antibodies

<400>  26
gagcccaaaa gctgtgacaa gacccacacc tgtcccccct gtcctgcccc tgagctcctt       60

gggggcccat ctgtgttcct gttccccccc aagcccaagg acaccctgat gatcagcaga      120

acccctgagg tgacctgtgt ggtggtggat gtgagccatg aggaccctga ggtgaagttc      180

aactggtatg tggatggggt ggaggtgcac aatgccaaga ccaagcctag agaggagcag      240

tacaacagca cctacagagt ggtgtctgtg ctgacagtgc tgcaccaaga ctggctgaat      300

ggcaaggagt acaagtgcaa ggtgagcaac aaggccctgc ctgcccccat tgagaagaca      360

atcagcaagg ccaaggggca gcctagagag ccccaagtgt acaccctgcc ccctagcaga      420

gaggagatga ccaagaacca agtgagcctg acctgcctgg tgaagggctt ctacccctct      480

gacattgctg tggagtggga gagcaatggg cagcctgaga acaactacaa gaccaccccc      540

cctgtgctgg actctgatgg cagcttcttc ctgtacagca agctgacagt ggacaagagc      600

agatggcagc aaggcaatgt gttcagctgc tctgtgatgc atgaggccct gcacaaccac      660

tacacacaga agagcctgag cctgagccct ggcaagtga                             699


