                             LISTA DE SECUENCIAS                              

<110> CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

<120> PROTEINA QUIMERICA QUE COMPRENDE EL DOMINIO DE UNION AL
      RECEPTOR DE LA PROTEINA DE LA ESPIGA DE CORONAVIRUS Y
      COMPOSICIONES QUE LA COMPRENDEN

<130> Secuencias-Vac

<140> CU 2020-0081
<141> 2020-11-04

<160> 11                            

<170> PatentIn Ver. 2.1

<210> 1
<211> 201
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena RBD

<400> 1
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg  
  1               5                  10                  15    

Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val  
             20                  25                  30        

Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys  
         35                  40                  45            

Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn  
     50                  55                  60                

Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile  
 65                  70                  75                  80

Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro  
                 85                  90                  95    

Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp  
            100                 105                 110        

Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys  
        115                 120                 125            

Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln  
    130                 135                 140                

Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe  
145                 150                 155                 160

Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln  
                165                 170                 175    

Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala  
            180                 185                 190        

Thr Val Cys Gly Pro Lys Lys Ser Thr                              
        195                 200    

<210> 2
<211> 207
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena RBDH

<400> 2
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg  
  1               5                  10                  15    

Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val  
             20                  25                  30        

Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys  
         35                  40                  45            

Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn  
     50                  55                  60                

Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile  
 65                  70                  75                  80

Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro  
                 85                  90                  95    

Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp  
            100                 105                 110        

Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys  
        115                 120                 125            

Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln  
    130                 135                 140                

Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe  
145                 150                 155                 160

Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln  
                165                 170                 175    

Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala  
            180                 185                 190        

Thr Val Cys Gly Pro Lys Lys Ser Thr His His His His His His      
        195                 200                 205        


<210> 3
<211> 222
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena RBDcmycH

<400> 3
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg  
  1               5                  10                  15    

Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val  
             20                  25                  30        

Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys  
         35                  40                  45            

Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn  
     50                  55                  60                

Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile  
 65                  70                  75                  80

Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro  
                 85                  90                  95    

Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp  
            100                 105                 110        

Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys  
        115                 120                 125            

Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln  
    130                 135                 140                

Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe  
145                 150                 155                 160

Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln  
                165                 170                 175    

Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala  
            180                 185                 190        

Thr Val Cys Gly Pro Lys Lys Ser Arg Glu Gln Lys Leu Ile Ser Glu  
        195                 200                 205            

Glu Asp Leu Asn Ser Ala Val Asp His His His His His His          
    210                 215                 220        

<210> 4
<211> 237
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena CRBDH6-P

<400> 4
Asn Trp Ser Phe Phe Ser Asn Ile Gly Gly Ser Ser Gly Gly Ser Asn  
  1               5                  10                  15    

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe  
             20                  25                  30        

Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala  
         35                  40                  45            

Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys  
     50                  55                  60                

Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val  
 65                  70                  75                  80

Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala  
                 85                  90                  95    

Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp  
            100                 105                 110        

Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser  
        115                 120                 125            

Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser  
    130                 135                 140                

Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala  
145                 150                 155                 160

Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro  
                165                 170                 175    

Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro  
            180                 185                 190        

Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr  
        195                 200                 205            

Val Cys Gly Pro Lys Lys Gly Gly Ser Gly Gly Ser Ser Ser Ser Ser  
    210                 215                 220                

Ser Ser Ser Ser Ser Ile Glu His His His His His His              
225                 230                 235        


<210> 5
<211> 227
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:Secuencia
      aminoacdica de la protena CRBDH6-H

<400> 5
Trp Ser Phe Phe Ser Asn Ile Gly Gly Ser Gly Gly Arg Phe Pro Asn  
  1               5                  10                  15    

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe  
             20                  25                  30        

Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala  
         35                  40                  45            

Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys  
     50                  55                  60                

Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val  
 65                  70                  75                  80

Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala  
                 85                  90                  95    

Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp  
            100                 105                 110        

Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser  
        115                 120                 125            

Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser  
    130                 135                 140                

Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala  
145                 150                 155                 160

Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro  
                165                 170                 175    

Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro  
            180                 185                 190        

Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr  
        195                 200                 205            

Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Ala Ala Ala His His His  
    210                 215                 220                

His His His                                                      
225        

<210> 6
<211> 209
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena CRBD

<400> 6
Asn Trp Ser Phe Phe Ser Asn Ile Asn Ile Thr Asn Leu Cys Pro Phe  
  1               5                  10                  15    

Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn  
             20                  25                  30        

Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn  
         35                  40                  45            

Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys  
     50                  55                  60                

Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile  
 65                  70                  75                  80

Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile  
                 85                  90                  95    

Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile  
            100                 105                 110        

Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn  
        115                 120                 125            

Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg  
    130                 135                 140                

Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly  
145                 150                 155                 160

Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln  
                165                 170                 175    

Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser  
            180                 185                 190        
Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser  
        195                 200                 205            

Thr                                                              
   
<210> 7
<211> 32
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      Oligonucletido empleado para la amplificacin del
      vector pPICZalfaA

<400> 7
gcatgctctt cagtttgtag ccttagacat ga                               32    

<210> 8
<211> 33
<212> ADN
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:
      Oligonucletido empleado para la amplificacin del
      vector pPICZalfaA

<400> 8
gcatgctctt cttgcttcag cctctctctt ctc                              33    

<210> 9
<211> 19
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:Secuencia
      aminoacdica del pptido seal de la protena
      humana LRP1

<400> 9
Met Leu Thr Pro Pro Leu Leu Leu Leu Leu Pro Leu Leu Ser Ala Leu  
  1               5                  10                  15    

Val Ala Ala                                                      

<210> 10
<211> 222
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial: Secuencia
      aminoacdica de la protena RBDH6-P

<400> 10
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg  
  1               5                  10                  15    

Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val  
             20                  25                  30        

Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys  
         35                  40                  45            

Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn  
     50                  55                  60                

Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile  
 65                  70                  75                  80

Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro  
                 85                  90                  95    

Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp  
            100                 105                 110        

Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys  
        115                 120                 125            

Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln  
    130                 135                 140                

Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe  
145                 150                 155                 160

Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln  
                165                 170                 175    

Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala  
            180                 185                 190        

Thr Val Cys Gly Pro Lys Lys Gly Gly Ser Gly Gly Ser Ser Ser Ser  
        195                 200                 205            

Ser Ser Ser Ser Ser Ser Ile Glu His His His His His His          
    210                 215                 220        


<210> 11
<211> 215
<212> PRT
<213> Secuencia Artificial

<220> 
<223> Descripcin de la Secuencia Artificial:  Secuencia
      aminoacdica de la protena RBDH6-H

<400> 11
Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn  
  1               5                  10                  15    

Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser  
             20                  25                  30        

Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser  
         35                  40                  45            

Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys  
     50                  55                  60                

Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val  
 65                  70                  75                  80

Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr  
                 85                  90                  95    

Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn  
            100                 105                 110        

Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu  
        115                 120                 125            

Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu  
    130                 135                 140                

Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn  
145                 150                 155                 160

Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val  
                165                 170                 175    

Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His  
            180                 185                 190        

Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Ala Ala  
        195                 200                 205            

Ala His His His His His His                                      
    210                 215


MsC. Mariela Vazquez Castillo
Agente Oficial, CIGB



