               SEQUENCE LISTING

<110> Boehringer Ingelheim International GmbH

<120> Viral vectors and nucleic acids for use in the treatment of ILD, PF-ILD and IPF

<130> P 112651

<160> 101

<170> BiSSAP 1.3.6

<210> 1
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 1
aaugcacccg ggcaaggauu ug                                             22


<210> 2
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 2
uccgucucag uuacuuuaua gc                                             22


<210> 3
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 3
acuggacuug gagucagaag g                                              21


<210> 4
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 4
acccgucccg uucguccccg ga                                             22


<210> 5
<211> 23
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 5
ucucacacag aaaucgcacc cgu                                            23


<210> 6
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 6
ucagugcacu acagaacuuu gu                                             22


<210> 7
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 7
agaucgaccg uguuauauuc gc                                             22


<210> 8
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 8
aauauaacac agauggccug u                                              21


<210> 9
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 9
ugucuugcag gccgucaugc a                                              21


<210> 10
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 10
ucagugcauc acagaacuuu gu                                             22


<210> 11
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 11
uaacagucuc cagucacggc ca                                             22


<210> 12
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 12
uauggcacug guagaauuca cu                                             22


<210> 13
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> mciroRNA (up)

<400> 13
uaguagaccg uauagcguac g                                              21


<210> 14
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 14
ucggauccgu cugagcuugg cu                                             22


<210> 15
<211> 23
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 15
accuuggcuc uagacugcuu acu                                            23


<210> 16
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (up)

<400> 16
ugagaacuga auuccauagg cu                                             22


<210> 17
<211> 23
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> mciroRNA (down)

<400> 17
aacauucaac gcugucggug agu                                            23


<210> 18
<211> 23
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 18
uacccuguag auccgaauuu gug                                            23


<210> 19
<211> 23
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 19
aacauucauu gcugucggug ggu                                            23


<210> 20
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 20
aauggcgcca cuaggguugu g                                              21


<210> 21
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 21
ugagaacuga auuccauggg uu                                             22


<210> 22
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 22
cuagacugag gcuccuugag g                                              21


<210> 23
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 23
uagcagcaca gaaauauugg c                                              21


<210> 24
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 24
gaguauuguu uccacugccu gg                                             22


<210> 25
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 25
gugaaauguu uaggaccacu ag                                             22


<210> 26
<211> 21
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 26
ccguccugag guuguugagc u                                              21


<210> 27
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 27
acaggcuguc ugaucccacg gu                                             22


<210> 28
<211> 22
<212> RNA
<213> Mus <mouse, genus>


<220> 
<223> microRNA (down)

<400> 28
guaaacaucc gacugaaagc uc                                             22


<210> 29
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide specific for lung - Seq ID No. 1 WO 2015/018860

<400> 29
Gly His Gly Tyr Phe 
1               5   

<210> 30
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> lung specifice peptide - Seq ID No. 2 WO 2015/018860

<400> 30
Glu Ser Gly His Gly Tyr Phe 
1               5           

<210> 31
<211> 744
<212> PRT
<213> Artificial Sequence


<220> 
<223> Seq ID No. 9 WO 2015/018860

<400> 31
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      
Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          
Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  
Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             
Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 
Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 
Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     
Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         
Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     
Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         
Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 
Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 
Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     
Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         
Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             
Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 
Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 
Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     
Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         
Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             
Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 
Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 
Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     
Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         
Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             
Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 
Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 
Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     
Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Gln Arg Gly Glu Ser Gly 
            580                 585                 590         
His Gly Tyr Phe Ala Gln Ala Ala Thr Ala Asp Val Asn Thr Gln Gly 
        595                 600                 605             
Val Leu Pro Gly Met Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly 
    610                 615                 620                 
Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly His Phe His Pro Ser 
625                 630                 635                 640 
Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu 
                645                 650                 655     
Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Ser Thr Thr Phe Ser Ala 
            660                 665                 670         
Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser 
        675                 680                 685             
Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn 
    690                 695                 700                 
Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asn Lys Ser Val Asn Val Asp 
705                 710                 715                 720 
Phe Thr Val Asp Thr Asn Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly 
                725                 730                 735     
Thr Arg Tyr Leu Thr Arg Asn Leu 
            740                 

<210> 32
<211> 1774
<212> DNA
<213> Artificial Sequence


<220> 
<223> CMV-mir181a-scAAV: Double stranded AAV vector genome for
      simultaneous expression of a cDNA (eGFP) and a miRNA

<400> 32
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgaaa cattcaacgc tgtcggtgag    1440

ttagtgaagc cacagatgta accatcgacc gttgattgta ccgtgcctac tgcctcggac    1500

ttcaaggggc tagaattcga gacttgttta ttgcagctta taatggttac aaataaagca    1560

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt    1620

ccaaactcat caatgtatct taacgcggcc gagatctcca ctccctctct gcgcgctcgc    1680

tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc    1740

tcagtgagcg agcgagcgcg cagctgcctg cagg                                1774


<210> 33
<211> 1719
<212> DNA
<213> Artificial Sequence


<220> 
<223> CMV-mir181a-mir181b-mir10a-scAAV: Double stranded AAV vector
      genome for simultaneous expression of three miRNAs

<400> 33
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaaggcg cgcctcgact tcttaaccca acagaaggct     660

cgagaaggta tattgctgtt gacagtgagc gaaacattca acgctgtcgg tgagttagtg     720

aagccacaga tgtaaccatc gaccgttgat tgtaccgtgc ctactgcctc ggacttcaag     780

gggctagaat tcgatcgact tcttaaccca acagaaggct cgagaaggta tattgctgtt     840

gacagtgagc gaaacattca ttgctgtcgg tgggtttagt gaagccacag atgtactcac     900

tgatcaatga atgcaaagtg cctactgcct cggacttcaa ggggctagaa ttcgatcgac     960

ttcttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag cgataccctg    1020

tagatccgaa tttgtgtagt gaagccacag atgtacaaat tcgtatctag gggaatagtg    1080

cctactgcct cggacttcaa ggggctagaa ttcgagaacg ggtggcatcc ctgtgacccc    1140

tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta    1200

ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg    1260

aggggggtgg tatggagcaa ggggcaagtt gggagaacaa cctgtagggc ctgcggggtc    1320

tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc    1380

tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg    1440

accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg    1500

ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga    1560

ttacaggcgt gaaccactgc tcccttccct gtccttagat ctccactccc tctctgcgcg    1620

ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg    1680

cggcctcagt gagcgagcga gcgcgcagct gcctgcagg                           1719


<210> 34
<211> 22
<212> RNA
<213> Homo sapiens


<220> 
<223> microRNA (up)

<400> 34
aaugcacccg ggcaaggauu cu                                             22


<210> 35
<211> 22
<212> RNA
<213> Homo sapiens


<220> 
<223> miRNA (up)

<400> 35
acuggacuug gagucagaag gc                                             22


<210> 36
<211> 21
<212> RNA
<213> Homo sapiens


<220> 
<223> miRNA (up)

<400> 36
uaacagucuc cagucacggc c                                              21


<210> 37
<211> 21
<212> RNA
<213> Homo sapiens


<220> 
<223> miRNA (down)

<400> 37
cuagacugaa gcuccuugag g                                              21


<210> 38
<211> 21
<212> RNA
<213> Homo sapiens


<220> 
<223> miRNA (down)

<400> 38
uagcagcaca gaaauauugg c                                              21


<210> 39
<211> 23
<212> RNA
<213> Homo sapiens


<220> 
<223> miRNA (down)

<400> 39
gggguauugu uuccgcugcc agg                                            23


<210> 40
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p 23 nt, miR-E backbone, Passenger position

<400> 40
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgtac     60

cctgtagatc cgaatttgtg tagtgaagcc acagatgtac acaaattcgg atctacaggg    120

tctgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 41
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p 23 nt, miR-E backbone, Guide position

<400> 41
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgaac     60

aaattcggat ctacagggta tagtgaagcc acagatgtat accctgtaga tccgaatttg    120

tgtgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 42
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p 22 nt, miR-E backbone, Passenger position

<400> 42
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgtac     60

cctgtagatc cgaatttgtt agtgaagcca cagatgtaac aaattcggat ctacagggtc    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 43
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p 22 nt, miR-E backbone, Guide position

<400> 43
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcca     60

aattcggatc tacagggtat agtgaagcca cagatgtata ccctgtagat ccgaatttgt    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 44
<211> 184
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p, natural pre-miRNA in miR-E backbone, Human
      (hsa-mir-10a MI0000266)

<400> 44
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttggat ctgtctgtct     60

tctgtatata ccctgtagat ccgaatttgt gtaaggaatt ttgtggtcac aaattcgtat    120

ctaggggaat atgtagttga cataaacact ccgctctctc ggacttcaag gggctagaat    180

tcga                                                                 184


<210> 45
<211> 184
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-10a-5p, natural pre-miRNA in miR-E backbone, Mouse
      (mmu-mir-10a MI0000685)

<400> 45
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttggac ctgtctgtct     60

tctgtatata ccctgtagat ccgaatttgt gtaaggaatt ttgtggtcac aaattcgtat    120

ctaggggaat atgtagttga cataaacact ccgctcactc ggacttcaag gggctagaat    180

tcga                                                                 184


<210> 46
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p 23 nt, miR-E backbone, Passenger position

<400> 46
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgaac     60

attcaacgct gtcggtgagt tagtgaagcc acagatgtaa ctcaccgaca gcgttgaatg    120

tgtgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 47
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p 23 nt, miR-E backbone, Guide position

<400> 47
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcct     60

caccgacagc gttgaatgtt tagtgaagcc acagatgtaa acattcaacg ctgtcggtga    120

gttgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 48
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p 22 nt, miR-E backbone, Passenger position

<400> 48
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgaac     60

attcaacgct gtcggtgagt agtgaagcca cagatgtact caccgacagc gttgaatgtg    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 49
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p 22 nt, miR-E backbone, Guide position

<400> 49
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgatc     60

accgacagcg ttgaatgttt agtgaagcca cagatgtaaa cattcaacgc tgtcggtgag    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 50
<211> 184
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p, natural pre-miRNA in miR-E backbone, Human
      (hsa-mir-181a-1 MI0000289)

<400> 50
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgtga gttttgaggt     60

tgcttcagtg aacattcaac gctgtcggtg agtttggaat taaaatcaaa accatcgacc    120

gttgattgta ccctatggct aaccatcatc tactccactc ggacttcaag gggctagaat    180

tcga                                                                 184


<210> 51
<211> 161
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181a-5p, natural pre-miRNA in miR-E backbone, Mouse
      (mmu-mir-181a-1 MI0000697)

<400> 51
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgggt tgcttcagtg     60

aacattcaac gctgtcggtg agtttggaat tcaaataaaa accatcgacc gttgattgta    120

ccctatagct aaccctcgga cttcaagggg ctagaattcg a                        161


<210> 52
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p 23 nt, miR-E backbone, Passenger position

<400> 52
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgaac     60

attcattgct gtcggtgggt tagtgaagcc acagatgtaa cccaccgaca gcaatgaatg    120

tgtgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 53
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p 23 nt, miR-E backbone, Guide position

<400> 53
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgccc     60

caccgacagc aatgaatgtt tagtgaagcc acagatgtaa acattcattg ctgtcggtgg    120

gttgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 54
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p 22 nt, miR-E backbone, Passenger position

<400> 54
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgaac     60

attcattgct gtcggtgggt agtgaagcca cagatgtacc caccgacagc aatgaatgtg    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 55
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p 22 nt, miR-E backbone, Guide position

<400> 55
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgacc     60

accgacagca atgaatgttt agtgaagcca cagatgtaaa cattcattgc tgtcggtggg    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 56
<211> 184
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p, natural pre-miRNA in miR-E backbone, Human
      (hsa-mir-181b-1 MI0000270)

<400> 56
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgcct gtgcagagat     60

tattttttaa aaggtcacaa tcaacattca ttgctgtcgg tgggttgaac tgtgtggaca    120

agctcactga acaatgaatg caactgtggc cccgcttctc ggacttcaag gggctagaat    180

tcga                                                                 184


<210> 57
<211> 154
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-181b-5p, natural pre-miRNA in miR-E backbone, Mouse
      (mmu-mir-181b-1 MI0000723)

<400> 57
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgagg tcacaatcaa     60

cattcattgc tgtcggtggg ttgaactgtg tagaaaagct cactgaacaa tgaatgcaac    120

tgtggccctc ggacttcaag gggctagaat tcga                                154


<210> 58
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p 23 nt, miR-E backbone, Passenger position

<400> 58
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgacc     60

ttggctctag actgcttact tagtgaagcc acagatgtaa gtaagcagtc tagagccaag    120

gctgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 59
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p 23 nt, miR-E backbone, Guide position

<400> 59
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcgt     60

aagcagtcta gagccaaggt tagtgaagcc acagatgtaa ccttggctct agactgctta    120

cttgcctact gcctcggact tcaaggggct agaattcga                           159


<210> 60
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p 22 nt, miR-E backbone, Passenger position

<400> 60
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgacc     60

ttggctctag actgcttact agtgaagcca cagatgtagt aagcagtcta gagccaaggc    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 61
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p 22 nt, miR-E backbone, Guide position

<400> 61
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgata     60

agcagtctag agccaaggtt agtgaagcca cagatgtaac cttggctcta gactgcttac    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 62
<211> 184
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p, natural pre-miRNA in miR-E backbone, Human
      (hsa-mir-212 MI0000288)

<400> 62
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgcgg ggcaccccgc     60

ccggacagcg cgccggcacc ttggctctag actgcttact gcccgggccg ccctcagtaa    120

cagtctccag tcacggccac cgacgcctgg ccccgccctc ggacttcaag gggctagaat    180

tcga                                                                 184


<210> 63
<211> 165
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-212-5p, natural pre-miRNA in miR-E backbone, Mouse
      (mmu-mir-212 MI0000696)

<400> 63
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgggg cagcgcgccg     60

gcaccttggc tctagactgc ttactgcccg ggccgccttc agtaacagtc tccagtcacg    120

gccaccgacg cctggcccct cggacttcaa ggggctagaa ttcga                    165


<210> 64
<211> 1773
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(23 nt in miR-E backbone)-SV40pA,
      Passenger position

<400> 64
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgaac attcattgct gtcggtgggt    1440

tagtgaagcc acagatgtaa cccaccgaca gcaatgaatg tgtgcctact gcctcggact    1500

tcaaggggct agaattcgag acttgtttat tgcagcttat aatggttaca aataaagcaa    1560

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc    1620

caaactcatc aatgtatctt aacgcggccg agatctccac tccctctctg cgcgctcgct    1680

cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct    1740

cagtgagcga gcgagcgcgc agctgcctgc agg                                 1773


<210> 65
<211> 1773
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(23 nt in miR-E backbone)-SV40pA, Guide
      position

<400> 65
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgccc caccgacagc aatgaatgtt    1440

tagtgaagcc acagatgtaa acattcattg ctgtcggtgg gttgcctact gcctcggact    1500

tcaaggggct agaattcgag acttgtttat tgcagcttat aatggttaca aataaagcaa    1560

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc    1620

caaactcatc aatgtatctt aacgcggccg agatctccac tccctctctg cgcgctcgct    1680

cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct    1740

cagtgagcga gcgagcgcgc agctgcctgc agg                                 1773


<210> 66
<211> 1771
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(22 nt in miR-E backbone)-SV40pA,
      Passenger position

<400> 66
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgaac attcattgct gtcggtgggt    1440

agtgaagcca cagatgtacc caccgacagc aatgaatgtg tgcctactgc ctcggacttc    1500

aaggggctag aattcgagac ttgtttattg cagcttataa tggttacaaa taaagcaata    1560

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca    1620

aactcatcaa tgtatcttaa cgcggccgag atctccactc cctctctgcg cgctcgctcg    1680

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca    1740

gtgagcgagc gagcgcgcag ctgcctgcag g                                   1771


<210> 67
<211> 1771
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(22 nt in miR-E backbone)-SV40pA, Guide
      position

<400> 67
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgacc accgacagca atgaatgttt    1440

agtgaagcca cagatgtaaa cattcattgc tgtcggtggg tgcctactgc ctcggacttc    1500

aaggggctag aattcgagac ttgtttattg cagcttataa tggttacaaa taaagcaata    1560

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca    1620

aactcatcaa tgtatcttaa cgcggccgag atctccactc cctctctgcg cgctcgctcg    1680

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca    1740

gtgagcgagc gagcgcgcag ctgcctgcag g                                   1771


<210> 68
<211> 1798
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(natural pre-miRNA, human)-SV40pA

<400> 68
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgcct gtgcagagat tattttttaa aaggtcacaa    1440

tcaacattca ttgctgtcgg tgggttgaac tgtgtggaca agctcactga acaatgaatg    1500

caactgtggc cccgcttctc ggacttcaag gggctagaat tcgagacttg tttattgcag    1560

cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt    1620

cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc ggccgagatc    1680

tccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga    1740

cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagg      1798


<210> 69
<211> 1768
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir181b-5p(natural pre-miRNA, mouse)-SV40pA

<400> 69
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgagg tcacaatcaa cattcattgc tgtcggtggg    1440

ttgaactgtg tagaaaagct cactgaacaa tgaatgcaac tgtggccctc ggacttcaag    1500

gggctagaat tcgagacttg tttattgcag cttataatgg ttacaaataa agcaatagca    1560

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac    1620

tcatcaatgt atcttaacgc ggccgagatc tccactccct ctctgcgcgc tcgctcgctc    1680

actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg    1740

agcgagcgag cgcgcagctg cctgcagg                                       1768


<210> 70
<211> 2091
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(all 23 nt in miR-E
      backbone), Passenger position

<400> 70
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgaac attcaacgct gtcggtgagt    1440

tagtgaagcc acagatgtaa ctcaccgaca gcgttgaatg tgtgcctact gcctcggact    1500

tcaaggggct agaattcgat cgacttctta acccaacaga aggctcgaga aggtatattg    1560

ctgttgacag tgagcgaaca ttcattgctg tcggtgggtt agtgaagcca cagatgtaac    1620

ccaccgacag caatgaatgt gtgcctactg cctcggactt caaggggcta gaattcgatc    1680

gacttcttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgtaccc    1740

tgtagatccg aatttgtgta gtgaagccac agatgtacac aaattcggat ctacagggtc    1800

tgcctactgc ctcggacttc aaggggctag aattcgagac ttgtttattg cagcttataa    1860

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    1920

ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcggccgag atctccactc    1980

cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg    2040

gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g             2091


<210> 71
<211> 2091
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(all 23 nt in miR-E
      backbone), Guide position

<400> 71
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgcct caccgacagc gttgaatgtt    1440

tagtgaagcc acagatgtaa acattcaacg ctgtcggtga gttgcctact gcctcggact    1500

tcaaggggct agaattcgat cgacttctta acccaacaga aggctcgaga aggtatattg    1560

ctgttgacag tgagcgcccc accgacagca atgaatgttt agtgaagcca cagatgtaaa    1620

cattcattgc tgtcggtggg ttgcctactg cctcggactt caaggggcta gaattcgatc    1680

gacttcttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgaacaa    1740

attcggatct acagggtata gtgaagccac agatgtatac cctgtagatc cgaatttgtg    1800

tgcctactgc ctcggacttc aaggggctag aattcgagac ttgtttattg cagcttataa    1860

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    1920

ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcggccgag atctccactc    1980

cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg    2040

gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g             2091


<210> 72
<211> 2085
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(all 22 nt in miR-E
      backbone), Passenger position

<400> 72
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgaac attcaacgct gtcggtgagt    1440

agtgaagcca cagatgtact caccgacagc gttgaatgtg tgcctactgc ctcggacttc    1500

aaggggctag aattcgatcg acttcttaac ccaacagaag gctcgagaag gtatattgct    1560

gttgacagtg agcgaacatt cattgctgtc ggtgggtagt gaagccacag atgtacccac    1620

cgacagcaat gaatgtgtgc ctactgcctc ggacttcaag gggctagaat tcgatcgact    1680

tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gtaccctgta    1740

gatccgaatt tgttagtgaa gccacagatg taacaaattc ggatctacag ggtctgccta    1800

ctgcctcgga cttcaagggg ctagaattcg agacttgttt attgcagctt ataatggtta    1860

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag    1920

ttgtggtttg tccaaactca tcaatgtatc ttaacgcggc cgagatctcc actccctctc    1980

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg    2040

cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcagg                    2085


<210> 73
<211> 2085
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(all 22 nt in miR-E
      backbone), Guide position

<400> 73
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgatc accgacagcg ttgaatgttt    1440

agtgaagcca cagatgtaaa cattcaacgc tgtcggtgag tgcctactgc ctcggacttc    1500

aaggggctag aattcgatcg acttcttaac ccaacagaag gctcgagaag gtatattgct    1560

gttgacagtg agcgaccacc gacagcaatg aatgtttagt gaagccacag atgtaaacat    1620

tcattgctgt cggtgggtgc ctactgcctc ggacttcaag gggctagaat tcgatcgact    1680

tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gccaaattcg    1740

gatctacagg gtatagtgaa gccacagatg tataccctgt agatccgaat ttgttgccta    1800

ctgcctcgga cttcaagggg ctagaattcg agacttgttt attgcagctt ataatggtta    1860

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag    1920

ttgtggtttg tccaaactca tcaatgtatc ttaacgcggc cgagatctcc actccctctc    1980

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg    2040

cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcagg                    2085


<210> 74
<211> 2091
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(all 23 nt in miR-E
      backbone), Passenger position

<400> 74
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgacc ttggctctag actgcttact    1440

tagtgaagcc acagatgtaa gtaagcagtc tagagccaag gctgcctact gcctcggact    1500

tcaaggggct agaattcgat cgacttctta acccaacaga aggctcgaga aggtatattg    1560

ctgttgacag tgagcgaaca ttcattgctg tcggtgggtt agtgaagcca cagatgtaac    1620

ccaccgacag caatgaatgt gtgcctactg cctcggactt caaggggcta gaattcgatc    1680

gacttcttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgtaccc    1740

tgtagatccg aatttgtgta gtgaagccac agatgtacac aaattcggat ctacagggtc    1800

tgcctactgc ctcggacttc aaggggctag aattcgagac ttgtttattg cagcttataa    1860

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    1920

ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcggccgag atctccactc    1980

cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg    2040

gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g             2091


<210> 75
<211> 2091
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(all 23 nt in miR-E
      backbone), Guide position

<400> 75
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgcgt aagcagtcta gagccaaggt    1440

tagtgaagcc acagatgtaa ccttggctct agactgctta cttgcctact gcctcggact    1500

tcaaggggct agaattcgat cgacttctta acccaacaga aggctcgaga aggtatattg    1560

ctgttgacag tgagcgcccc accgacagca atgaatgttt agtgaagcca cagatgtaaa    1620

cattcattgc tgtcggtggg ttgcctactg cctcggactt caaggggcta gaattcgatc    1680

gacttcttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt gagcgaacaa    1740

attcggatct acagggtata gtgaagccac agatgtatac cctgtagatc cgaatttgtg    1800

tgcctactgc ctcggacttc aaggggctag aattcgagac ttgtttattg cagcttataa    1860

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    1920

ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcggccgag atctccactc    1980

cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg    2040

gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g             2091


<210> 76
<211> 2085
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(all 22 nt in miR-E
      backbone), Passenger position

<400> 76
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgacc ttggctctag actgcttact    1440

agtgaagcca cagatgtagt aagcagtcta gagccaaggc tgcctactgc ctcggacttc    1500

aaggggctag aattcgatcg acttcttaac ccaacagaag gctcgagaag gtatattgct    1560

gttgacagtg agcgaacatt cattgctgtc ggtgggtagt gaagccacag atgtacccac    1620

cgacagcaat gaatgtgtgc ctactgcctc ggacttcaag gggctagaat tcgatcgact    1680

tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gtaccctgta    1740

gatccgaatt tgttagtgaa gccacagatg taacaaattc ggatctacag ggtctgccta    1800

ctgcctcgga cttcaagggg ctagaattcg agacttgttt attgcagctt ataatggtta    1860

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag    1920

ttgtggtttg tccaaactca tcaatgtatc ttaacgcggc cgagatctcc actccctctc    1980

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg    2040

cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcagg                    2085


<210> 77
<211> 2085
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(all 22 nt in miR-E
      backbone), Guide position

<400> 77
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgaca gtgagcgata agcagtctag agccaaggtt    1440

agtgaagcca cagatgtaac cttggctcta gactgcttac tgcctactgc ctcggacttc    1500

aaggggctag aattcgatcg acttcttaac ccaacagaag gctcgagaag gtatattgct    1560

gttgacagtg agcgaccacc gacagcaatg aatgtttagt gaagccacag atgtaaacat    1620

tcattgctgt cggtgggtgc ctactgcctc ggacttcaag gggctagaat tcgatcgact    1680

tcttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gccaaattcg    1740

gatctacagg gtatagtgaa gccacagatg tataccctgt agatccgaat ttgttgccta    1800

ctgcctcgga cttcaagggg ctagaattcg agacttgttt attgcagctt ataatggtta    1860

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag    1920

ttgtggtttg tccaaactca tcaatgtatc ttaacgcggc cgagatctcc actccctctc    1980

tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg    2040

cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcagg                    2085


<210> 78
<211> 2166
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(natural pre-miRNAs in
      miR-E backbone), Human

<400> 78
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgtga gttttgaggt tgcttcagtg aacattcaac    1440

gctgtcggtg agtttggaat taaaatcaaa accatcgacc gttgattgta ccctatggct    1500

aaccatcatc tactccactc ggacttcaag gggctagaat tcgatcgact tcttaaccca    1560

acagaaggct cgagaaggta tattgctgtt gcctgtgcag agattatttt ttaaaaggtc    1620

acaatcaaca ttcattgctg tcggtgggtt gaactgtgtg gacaagctca ctgaacaatg    1680

aatgcaactg tggccccgct tctcggactt caaggggcta gaattcgatc gacttcttaa    1740

cccaacagaa ggctcgagaa ggtatattgc tgttggatct gtctgtcttc tgtatatacc    1800

ctgtagatcc gaatttgtgt aaggaatttt gtggtcacaa attcgtatct aggggaatat    1860

gtagttgaca taaacactcc gctctctcgg acttcaaggg gctagaattc gagacttgtt    1920

tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc    1980

atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgg    2040

ccgagatctc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg    2100

tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc    2160

tgcagg                                                               2166


<210> 79
<211> 2113
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-181a-mir181b-mir10a(natural pre-miRNAs in
      miR-E backbone), Mouse

<400> 79
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgggt tgcttcagtg aacattcaac gctgtcggtg    1440

agtttggaat tcaaataaaa accatcgacc gttgattgta ccctatagct aaccctcgga    1500

cttcaagggg ctagaattcg atcgacttct taacccaaca gaaggctcga gaaggtatat    1560

tgctgttgag gtcacaatca acattcattg ctgtcggtgg gttgaactgt gtagaaaagc    1620

tcactgaaca atgaatgcaa ctgtggccct cggacttcaa ggggctagaa ttcgatcgac    1680

ttcttaaccc aacagaaggc tcgagaaggt atattgctgt tggacctgtc tgtcttctgt    1740

atataccctg tagatccgaa tttgtgtaag gaattttgtg gtcacaaatt cgtatctagg    1800

ggaatatgta gttgacataa acactccgct cactcggact tcaaggggct agaattcgag    1860

acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa    1920

ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt    1980

aacgcggccg agatctccac tccctctctg cgcgctcgct cgctcactga ggccgggcga    2040

ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc    2100

agctgcctgc agg                                                       2113


<210> 80
<211> 2166
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(natural pre-miRNAs in
      miR-E backbone), Human

<400> 80
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgcgg ggcaccccgc ccggacagcg cgccggcacc    1440

ttggctctag actgcttact gcccgggccg ccctcagtaa cagtctccag tcacggccac    1500

cgacgcctgg ccccgccctc ggacttcaag gggctagaat tcgatcgact tcttaaccca    1560

acagaaggct cgagaaggta tattgctgtt gcctgtgcag agattatttt ttaaaaggtc    1620

acaatcaaca ttcattgctg tcggtgggtt gaactgtgtg gacaagctca ctgaacaatg    1680

aatgcaactg tggccccgct tctcggactt caaggggcta gaattcgatc gacttcttaa    1740

cccaacagaa ggctcgagaa ggtatattgc tgttggatct gtctgtcttc tgtatatacc    1800

ctgtagatcc gaatttgtgt aaggaatttt gtggtcacaa attcgtatct aggggaatat    1860

gtagttgaca taaacactcc gctctctcgg acttcaaggg gctagaattc gagacttgtt    1920

tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc    1980

atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgg    2040

ccgagatctc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg    2100

tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc    2160

tgcagg                                                               2166


<210> 81
<211> 2117
<212> DNA
<213> Artificial Sequence


<220> 
<223> >scAAV-CMV-eGFP-mir-212-5p-mir181b-mir10a(natural pre-miRNAs in
      miR-E backbone), Mouse

<400> 81
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc      60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca     120

actccatcac taggggttcc tgcggccgct cgacccccta aaatgggcaa acattgcaag     180

caaacagcaa acacacagcc ctccctgcct gctgaccttg gagctggggc agaggtcaga     240

gacctctctg ggcccatgcc acctccaaca tccactcgac cccttggaat ttcggtggag     300

aggagcagag gttgtcctgg cgtggtttag gtagtgtgag aggggaatga ctcctttcgg     360

taagtgcagt ggaagctgta cactgcccag gcaaagcgtc cgggcagcgt aggcgggcga     420

ctcagatccc agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt     480

ggttaatatt caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga     540

cgaggacagg gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc     600

cggactctaa gaggtacctt aattaagcca ccatggtgtc caagggcgag gaactgttca     660

ccggcgtggt gcccatcctg gtggaactgg atggcgacgt gaacggccac aagttcagcg     720

tgtccggcga gggcgaaggc gacgccacat atggcaagct gaccctgaag ttcatctgca     780

ccaccggcaa gctgcccgtg ccttggccta ccctcgtgac cacactgacc tacggcgtgc     840

agtgcttcag cagatacccc gaccatatga agcagcacga cttcttcaag agcgccatgc     900

ccgagggcta cgtgcaggaa cggaccatct tctttaagga cgacggcaac tacaagacca     960

gggccgaagt gaagttcgag ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg    1020

acttcaaaga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca    1080

acgtgtacat catggccgac aagcagaaaa acggcatcaa agtgaacttc aagatccggc    1140

acaacatcga ggacggctcc gtgcagctgg ccgaccacta ccagcagaac acccccatcg    1200

gagatggccc cgtgctgctg cccgacaacc actacctgag cacacagagc gccctgagca    1260

aggaccccaa cgagaagcgg gaccacatgg tgctgctgga atttgtgacc gccgctggca    1320

tcaccctggg catggacgag ctgtacaaat gaggcgcgcc tcgacttctt aacccaacag    1380

aaggctcgag aaggtatatt gctgttgggg cagcgcgccg gcaccttggc tctagactgc    1440

ttactgcccg ggccgccttc agtaacagtc tccagtcacg gccaccgacg cctggcccct    1500

cggacttcaa ggggctagaa ttcgatcgac ttcttaaccc aacagaaggc tcgagaaggt    1560

atattgctgt tgaggtcaca atcaacattc attgctgtcg gtgggttgaa ctgtgtagaa    1620

aagctcactg aacaatgaat gcaactgtgg ccctcggact tcaaggggct agaattcgat    1680

cgacttctta acccaacaga aggctcgaga aggtatattg ctgttggacc tgtctgtctt    1740

ctgtatatac cctgtagatc cgaatttgtg taaggaattt tgtggtcaca aattcgtatc    1800

taggggaata tgtagttgac ataaacactc cgctcactcg gacttcaagg ggctagaatt    1860

cgagacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc    1920

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta    1980

tcttaacgcg gccgagatct ccactccctc tctgcgcgct cgctcgctca ctgaggccgg    2040

gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc    2100

gcgcagctgc ctgcagg                                                   2117


<210> 82
<211> 736
<212> PRT
<213> Artificial Sequence


<220> 
<223> AAV6.2 VP1

<400> 82
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly 
145                 150                 155                 160 
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro 
            180                 185                 190         
Ala Thr Pro Ala Ala Val Gly Pro Thr Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala 
    210                 215                 220                 
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     
Tyr Lys Gln Ile Ser Ser Ala Ser Thr Gly Ala Ser Asn Asp Asn His 
            260                 265                 270         
Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe 
        275                 280                 285             
His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn 
    290                 295                 300                 
Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln 
305                 310                 315                 320 
Val Lys Glu Val Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn 
                325                 330                 335     
Leu Thr Ser Thr Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro 
            340                 345                 350         
Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala 
        355                 360                 365             
Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly 
    370                 375                 380                 
Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro 
385                 390                 395                 400 
Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe 
                405                 410                 415     
Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp 
            420                 425                 430         
Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Asn Arg 
        435                 440                 445             
Thr Gln Asn Gln Ser Gly Ser Ala Gln Asn Lys Asp Leu Leu Phe Ser 
    450                 455                 460                 
Arg Gly Ser Pro Ala Gly Met Ser Val Gln Pro Lys Asn Trp Leu Pro 
465                 470                 475                 480 
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Lys Thr Asp Asn 
                485                 490                 495     
Asn Asn Ser Asn Phe Thr Trp Thr Gly Ala Ser Lys Tyr Asn Leu Asn 
            500                 505                 510         
Gly Arg Glu Ser Ile Ile Asn Pro Gly Thr Ala Met Ala Ser His Lys 
        515                 520                 525             
Asp Asp Lys Asp Lys Phe Phe Pro Met Ser Gly Val Met Ile Phe Gly 
    530                 535                 540                 
Lys Glu Ser Ala Gly Ala Ser Asn Thr Ala Leu Asp Asn Val Met Ile 
545                 550                 555                 560 
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Arg 
                565                 570                 575     
Phe Gly Thr Val Ala Val Asn Leu Gln Ser Ser Ser Thr Asp Pro Ala 
            580                 585                 590         
Thr Gly Asp Val His Val Met Gly Ala Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 
Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu 
625                 630                 635                 640 
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     
Asn Pro Pro Ala Glu Phe Ser Ala Thr Lys Phe Ala Ser Phe Ile Thr 
            660                 665                 670         
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Val Gln Tyr Thr Ser Asn 
    690                 695                 700                 
Tyr Ala Lys Ser Ala Asn Val Asp Phe Thr Val Asp Asn Asn Gly Leu 
705                 710                 715                 720 
Tyr Thr Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu 
                725                 730                 735     



<210> 83
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir- Ren713, neutral control, mirE backbone

<400> 83
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcag     60

gaattataat gcttatctat agtgaagcca cagatgtata gataagcatt ataattccta    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 84
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-181a stem-loop, miR-E context

<400> 84
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgtttgag ttttgaggtt     60

gcttcagtga acattcaacg ctgtcggtga gtttggaatt aaaatcaaaa ccatcgaccg    120

ttgattgtac cctatggcta accatcatct actccatcgg acttcaaggg gctagaattc    180

ga                                                                   182


<210> 85
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-212 stem-loop, miR-E context

<400> 85
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttcggg gcaccccgcc     60

cggacagcgc gccggcacct tggctctaga ctgcttactg cccgggccgc cctcagtaac    120

agtctccagt cacggccacc gacgcctggc cccgcctcgg acttcaaggg gctagaattc    180

ga                                                                   182


<210> 86
<211> 157
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a-3p, miR-E backbone

<400> 86
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcaa     60

ccgatttcag atggtgctat agtgaagcca cagatgtata gcaccatctg aaatcggtta    120

tgcctactgc ctcggacttc aaggggctag aattcga                             157


<210> 87
<211> 159
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-181a stem-loop, miR-E context, Mouse

<400> 87
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttggtt gcttcagtga     60

acattcaacg ctgtcggtga gtttggaatt caaataaaaa ccatcgaccg ttgattgtac    120

cctatagcta acctcggact tcaaggggct agaattcga                           159


<210> 88
<211> 163
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-212 stem-loop, miR-E context, Mouse

<400> 88
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgggc agcgcgccgg     60

caccttggct ctagactgct tactgcccgg gccgccttca gtaacagtct ccagtcacgg    120

ccaccgacgc ctggccctcg gacttcaagg ggctagaatt cga                      163


<210> 89
<211> 136
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a stem-loop, miR-E context

<400> 89
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttatga ctgatttctt     60

ttggtgttca gagtcaatat aattttctag caccatctga aatcggttat tcggacttca    120

aggggctaga attcga                                                    136


<210> 90
<211> 138
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a, natural pre-miRNA in miR-E backbone

<400> 90
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgatg actgatttct     60

tttggtgttc agagtcaata taattttcta gcaccatctg aaatcggtta tctcggactt    120

caaggggcta gaattcga                                                  138


<210> 91
<211> 3846
<212> DNA
<213> Artificial Sequence (plasmid)


<220> 
<223> >#2760_pAAVsc_CMV-miR212-5p\22nt\in mir-E backbone, circular

<220> 
<221> misc_feature
<222> 1
<223> /note="first nucleotide ITR"

<220> 
<221> misc_feature
<222> 150..1131
<223> /note="CMV-miR-212-polyA expression cassete"

<220> 
<221> misc_feature
<222> 753..909
<223> /note="see Seq ID NO: 61"

<220> 
<221> misc_feature
<222> 1249
<223> /note="last nucleotide ITR"

<400> 91
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccgct agttattaat agtaatcaat tacggggtca      180

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct      240

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta      300

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac      360

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt      420

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag      480

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat      540

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat      600

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc      660

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt      720

ttagtgaacc gtcagatcgg taccggcgcg cctcgacttc ttaacccaac agaaggctcg      780

agaaggtata ttgctgttga cagtgagcga taagcagtct agagccaagg ttagtgaagc      840

cacagatgta accttggctc tagactgctt actgcctact gcctcggact tcaaggggct      900

agaattcgac gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc      960

cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg     1020

catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca     1080

agggggagga ttgggaagac aatagcaggc atgctgggga gagctcagat ctccactccc     1140

tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc     1200

tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg     1260

cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat     1320

agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga     1380

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg     1440

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat     1500

ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg     1560

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata     1620

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt     1680

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat     1740

ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa     1800

tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc     1860

cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga     1920

gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg     1980

tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg     2040

gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa     2100

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     2160

agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     2220

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     2280

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     2340

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     2400

tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     2460

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     2520

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     2580

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     2640

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     2700

cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     2760

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     2820

tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg     2880

ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta     2940

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     3000

gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga     3060

ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc     3120

tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa     3180

agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa     3240

aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc     3300

cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt     3360

agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc     3420

tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac     3480

gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca     3540

gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg     3600

ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag     3660

gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt     3720

ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat     3780

ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc     3840

acatgt                                                                3846


<210> 92
<211> 22
<212> RNA
<213> Artificial Sequence


<220> 
<223> hsa miR-29a-3p

<400> 92
uagcaccauc ugaaaucggu ua                                             22


<210> 93
<211> 529
<212> DNA
<213> Artificial Sequence


<220> 
<223> >mir-29a\stem\loop-mir181a\stem\loop-bGH\pA

<400> 93
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttatga ctgatttctt      60

ttggtgttca gagtcaatat aattttctag caccatctga aatcggttat tcggacttca     120

aggggctaga attcgatcga cttcttaacc caacagaagg ctcgagaagg tatattgctg     180

tttgagtttt gaggttgctt cagtgaacat tcaacgctgt cggtgagttt ggaattaaaa     240

tcaaaaccat cgaccgttga ttgtacccta tggctaacca tcatctactc catcggactt     300

caaggggcta gaattcgacg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     360

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     420

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     480

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctgggga                 529


<210> 94
<211> 529
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a\stem\loop-mir212\stem\loop-bGH\pA

<400> 94
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttatga ctgatttctt      60

ttggtgttca gagtcaatat aattttctag caccatctga aatcggttat tcggacttca     120

aggggctaga attcgatcga cttcttaacc caacagaagg ctcgagaagg tatattgctg     180

ttcggggcac cccgcccgga cagcgcgccg gcaccttggc tctagactgc ttactgcccg     240

ggccgccctc agtaacagtc tccagtcacg gccaccgacg cctggccccg cctcggactt     300

caaggggcta gaattcgacg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     360

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     420

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     480

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctgggga                 529


<210> 95
<211> 527
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a-3p-mir-181a-5p\23\nt,\miR-E\backbone,\Guide\position,\bGH
      \pA

<400> 95
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcaa      60

ccgatttcag atggtgctat agtgaagcca cagatgtata gcaccatctg aaatcggtta     120

tgcctactgc ctcggacttc aaggggctag aattcgatcg acttcttaac ccaacagaag     180

gctcgagaag gtatattgct gttgacagtg agcgcctcac cgacagcgtt gaatgtttag     240

tgaagccaca gatgtaaaca ttcaacgctg tcggtgagtt gcctactgcc tcggacttca     300

aggggctaga attcgacgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     360

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     420

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     480

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctgggga                   527


<210> 96
<211> 525
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a-3p-mir-181a-5p\22\nt,\miR-E\backbone,\Guide\position,\bGH
      \pA

<400> 96
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcaa      60

ccgatttcag atggtgctat agtgaagcca cagatgtata gcaccatctg aaatcggtta     120

tgcctactgc ctcggacttc aaggggctag aattcgatcg acttcttaac ccaacagaag     180

gctcgagaag gtatattgct gttgacagtg agcgatcacc gacagcgttg aatgtttagt     240

gaagccacag atgtaaacat tcaacgctgt cggtgagtgc ctactgcctc ggacttcaag     300

gggctagaat tcgacgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc     360

cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga     420

aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga     480

cagcaagggg gaggattggg aagacaatag caggcatgct gggga                     525


<210> 97
<211> 527
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a-3p-mir-212-5p\23\nt,\miR-E\backbone,\Guide\position,\bGH\
      pA

<400> 97
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcaa      60

ccgatttcag atggtgctat agtgaagcca cagatgtata gcaccatctg aaatcggtta     120

tgcctactgc ctcggacttc aaggggctag aattcgatcg acttcttaac ccaacagaag     180

gctcgagaag gtatattgct gttgacagtg agcgcgtaag cagtctagag ccaaggttag     240

tgaagccaca gatgtaacct tggctctaga ctgcttactt gcctactgcc tcggacttca     300

aggggctaga attcgacgac tgtgccttct agttgccagc catctgttgt ttgcccctcc     360

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag     420

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag     480

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctgggga                   527


<210> 98
<211> 525
<212> DNA
<213> Artificial Sequence


<220> 
<223> mir-29a-3p-mir-212-5p\22\nt,\miR-E\backbone,\Guide\position,\bGH\
      pA

<400> 98
tcgacttctt aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcaa      60

ccgatttcag atggtgctat agtgaagcca cagatgtata gcaccatctg aaatcggtta     120

tgcctactgc ctcggacttc aaggggctag aattcgatcg acttcttaac ccaacagaag     180

gctcgagaag gtatattgct gttgacagtg agcgataagc agtctagagc caaggttagt     240

gaagccacag atgtaacctt ggctctagac tgcttactgc ctactgcctc ggacttcaag     300

gggctagaat tcgacgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc     360

cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga     420

aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga     480

cagcaagggg gaggattggg aagacaatag caggcatgct gggga                     525


<210> 99
<211> 22
<212> RNA
<213> Artificial Sequence


<220> 
<223> hsa-mir-212-5p, 22 nt (having a deletion of 1 nt at the
      3'-terminus)

<400> 99
accuuggcuc uagacugcuu ac                                             22


<210> 100
<211> 22
<212> RNA
<213> Artificial Sequence


<220> 
<223> hsa-miR-181a-5p, 22nt (having a deletion of 1 nt at the
      3'-terminus)

<400> 100
aacauucaac gcugucggug ag                                             22


<210> 101
<211> 22
<212> RNA
<213> Artificial Sequence


<220> 
<223> hsa-mir-181b-5p, 22nt (having a deletion of 1 nt at the
      3'-terminus)

<400> 101
aacauucauu gcugucggug gg                                             22


