                         SEQUENCE LISTING

<110>  NEW YORK UNIVERSITY
 
<120>  ENHANCEMENT OF PREDICTABLE AND TEMPLATE-FREE GENE EDITING BY THE 
       ASSOCIATION OF CAS WITH DNA POLYMERASE

<130>  058636.00417

<150>  63/109,909
<151>  2020-11-05

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  1
caagctgaag gtgaccaggg cgg                                               23


<210>  2
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  2
caagctgaag gtgaccaagg gcgg                                              24


<210>  3
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  

CRISPR related sequence

<400>  3
caagctgaag gtgaccaggg                                                   20


<210>  4
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  4
gttcgattcc actggtcccg cc                                                22


<210>  5
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  5
atttatagcc caagatttcc                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  6
gcctgcttcc tcacagcttg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  7
ttcttgaacc aggaaatctt                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  8
agagtaacag tctgagtagg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  9
cctgcagggt ggcctcacct                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  10
ggggccaggt ggccaaggtg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  11
aaaatgtaca aggaccgaca                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  12
accagagtaa cagtctgagt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR related sequence

<400>  13
tataaaatca cagagggtga                                                   20


<210>  14
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  self cleaving peptide sequence

<400>  14

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  15
<211>  19
<212>  PRT
<213>  artificial sequence

<220>
<223>  self cleaving peptide sequence

<400>  15

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  16
<211>  20
<212>  PRT
<213>  artificial sequence

<220>
<223>  self cleaving peptide sequence

<400>  16

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  17
<211>  22
<212>  PRT
<213>  artificial sequence

<220>
<223>  self cleaving peptide sequence

<400>  17

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  18
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  linker

<400>  18

Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Gly 
        


<210>  19
<211>  897
<212>  PRT
<213>  Bacteriophage T4

<400>  19

Lys Glu Phe Tyr Ile Ser Ile Glu Thr Val Gly Asn Asn Ile Val Glu 
1               5                   10                  15      


Arg Tyr Ile Asp Glu Asn Gly Lys Glu Arg Thr Arg Glu Val Glu Tyr 
            20                  25                  30          


Leu Pro Thr Met Phe Arg His Cys Lys Glu Glu Ser Lys Tyr Lys Asp 
        35                  40                  45              


Ile Tyr Gly Lys Asn Cys Ala Pro Gln Lys Phe Pro Ser Met Lys Asp 
    50                  55                  60                  


Ala Arg Asp Trp Met Lys Arg Met Glu Asp Ile Gly Leu Glu Ala Leu 
65                  70                  75                  80  


Gly Met Asn Asp Phe Lys Leu Ala Tyr Ile Ser Asp Thr Tyr Gly Ser 
                85                  90                  95      


Glu Ile Val Tyr Asp Arg Lys Phe Val Arg Val Ala Asn Cys Asp Ile 
            100                 105                 110         


Glu Val Thr Gly Asp Lys Phe Pro Asp Pro Met Lys Ala Glu Tyr Glu 
        115                 120                 125             


Ile Asp Ala Ile Thr His Tyr Asp Ser Ile Asp Asp Arg Phe Tyr Val 
    130                 135                 140                 


Phe Asp Leu Leu Asn Ser Met Tyr Gly Ser Val Ser Lys Trp Asp Ala 
145                 150                 155                 160 


Lys Leu Ala Ala Lys Leu Asp Cys Glu Gly Gly Asp Glu Val Pro Gln 
                165                 170                 175     


Glu Ile Leu Asp Arg Val Ile Tyr Met Pro Phe Asp Asn Glu Arg Asp 
            180                 185                 190         


Met Leu Met Glu Tyr Ile Asn Leu Trp Glu Gln Lys Arg Pro Ala Ile 
        195                 200                 205             


Phe Thr Gly Trp Asn Ile Glu Gly Phe Asp Val Pro Tyr Ile Met Asn 
    210                 215                 220                 


Arg Val Lys Met Ile Leu Gly Glu Arg Ser Met Lys Arg Phe Ser Pro 
225                 230                 235                 240 


Ile Gly Arg Val Lys Ser Lys Leu Ile Gln Asn Met Tyr Gly Ser Lys 
                245                 250                 255     


Glu Ile Tyr Ser Ile Asp Gly Val Ser Ile Leu Asp Tyr Leu Asp Leu 
            260                 265                 270         


Tyr Lys Lys Phe Ala Phe Thr Asn Leu Pro Ser Phe Ser Leu Glu Ser 
        275                 280                 285             


Val Ala Gln His Glu Thr Lys Lys Gly Lys Leu Pro Tyr Asp Gly Pro 
    290                 295                 300                 


Ile Asn Lys Leu Arg Glu Thr Asn His Gln Arg Tyr Ile Ser Tyr Asn 
305                 310                 315                 320 


Ile Ile Asp Val Glu Ser Val Gln Ala Ile Asp Lys Ile Arg Gly Phe 
                325                 330                 335     


Ile Asp Leu Val Leu Ser Met Ser Tyr Tyr Ala Lys Met Pro Phe Ser 
            340                 345                 350         


Gly Val Met Ser Pro Ile Lys Thr Trp Asp Ala Ile Ile Phe Asn Ser 
        355                 360                 365             


Leu Lys Gly Glu His Lys Val Ile Pro Gln Gln Gly Ser His Val Lys 
    370                 375                 380                 


Gln Ser Phe Pro Gly Ala Phe Val Phe Glu Pro Lys Pro Ile Ala Arg 
385                 390                 395                 400 


Arg Tyr Ile Met Ser Phe Asp Leu Thr Ser Leu Tyr Pro Ser Ile Ile 
                405                 410                 415     


Arg Gln Val Asn Ile Ser Pro Glu Thr Ile Arg Gly Gln Phe Lys Val 
            420                 425                 430         


His Pro Ile His Glu Tyr Ile Ala Gly Thr Ala Pro Lys Pro Ser Asp 
        435                 440                 445             


Glu Tyr Ser Cys Ser Pro Asn Gly Trp Met Tyr Asp Lys His Gln Glu 
    450                 455                 460                 


Gly Ile Ile Pro Lys Glu Ile Ala Lys Val Phe Phe Gln Arg Lys Asp 
465                 470                 475                 480 


Trp Lys Lys Lys Met Phe Ala Glu Glu Met Asn Ala Glu Ala Ile Lys 
                485                 490                 495     


Lys Ile Ile Met Lys Gly Ala Gly Ser Cys Ser Thr Lys Pro Glu Val 
            500                 505                 510         


Glu Arg Tyr Val Lys Phe Ser Asp Asp Phe Leu Asn Glu Leu Ser Asn 
        515                 520                 525             


Tyr Thr Glu Ser Val Leu Asn Ser Leu Ile Glu Glu Cys Glu Lys Ala 
    530                 535                 540                 


Ala Thr Leu Ala Asn Thr Asn Gln Leu Asn Arg Lys Ile Leu Ile Asn 
545                 550                 555                 560 


Ser Leu Tyr Gly Ala Leu Gly Asn Ile His Phe Arg Tyr Tyr Asp Leu 
                565                 570                 575     


Arg Asn Ala Thr Ala Ile Thr Ile Phe Gly Gln Val Gly Ile Gln Trp 
            580                 585                 590         


Ile Ala Arg Lys Ile Asn Glu Tyr Leu Asn Lys Val Cys Gly Thr Asn 
        595                 600                 605             


Asp Glu Asp Phe Ile Ala Ala Gly Asp Thr Asp Ser Val Tyr Val Cys 
    610                 615                 620                 


Val Asp Lys Val Ile Glu Lys Val Gly Leu Asp Arg Phe Lys Glu Gln 
625                 630                 635                 640 


Asn Asp Leu Val Glu Phe Met Asn Gln Phe Gly Lys Lys Lys Met Glu 
                645                 650                 655     


Pro Met Ile Asp Val Ala Tyr Arg Glu Leu Cys Asp Tyr Met Asn Asn 
            660                 665                 670         


Arg Glu His Leu Met His Met Asp Arg Glu Ala Ile Ser Cys Pro Pro 
        675                 680                 685             


Leu Gly Ser Lys Gly Val Gly Gly Phe Trp Lys Ala Lys Lys Arg Tyr 
    690                 695                 700                 


Ala Leu Asn Val Tyr Asp Met Glu Asp Lys Arg Phe Ala Glu Pro His 
705                 710                 715                 720 


Leu Lys Ile Met Gly Met Glu Thr Gln Gln Ser Ser Thr Pro Lys Ala 
                725                 730                 735     


Val Gln Glu Ala Leu Glu Glu Ser Ile Arg Arg Ile Leu Gln Glu Gly 
            740                 745                 750         


Glu Glu Ser Val Gln Glu Tyr Tyr Lys Asn Phe Glu Lys Glu Tyr Arg 
        755                 760                 765             


Gln Leu Asp Tyr Lys Val Ile Ala Glu Val Lys Thr Ala Asn Asp Ile 
    770                 775                 780                 


Ala Lys Tyr Asp Asp Lys Gly Trp Pro Gly Phe Lys Cys Pro Phe His 
785                 790                 795                 800 


Ile Arg Gly Val Leu Thr Tyr Arg Arg Ala Val Ser Gly Leu Gly Val 
                805                 810                 815     


Ala Pro Ile Leu Asp Gly Asn Lys Val Met Val Leu Pro Leu Arg Glu 
            820                 825                 830         


Gly Asn Pro Phe Gly Asp Lys Cys Ile Ala Trp Pro Ser Gly Thr Glu 
        835                 840                 845             


Leu Pro Lys Glu Ile Arg Ser Asp Val Leu Ser Trp Ile Asp His Ser 
    850                 855                 860                 


Thr Leu Phe Gln Lys Ser Phe Val Lys Pro Leu Ala Gly Met Cys Glu 
865                 870                 875                 880 


Ser Ala Gly Met Asp Tyr Glu Glu Lys Ala Ser Leu Asp Phe Leu Phe 
                885                 890                 895     


Gly 
    


<210>  20
<211>  130
<212>  PRT
<213>  artificial sequence

<220>
<223>  MS2 binding protein

<400>  20

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr 
    130 


<210>  21
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  nuclear localization signal

<400>  21

Gly Pro Lys Lys Lys Arg Lys Val Ala Ala Ala 
1               5                   10      


<210>  22
<211>  1065
<212>  PRT
<213>  artificial sequence

<220>
<223>  fusion protein

<400>  22

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  


Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      


Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         


Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             


Ile Tyr Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Lys Glu Phe Tyr Ile 
145                 150                 155                 160 


Ser Ile Glu Thr Val Gly Asn Asn Ile Val Glu Arg Tyr Ile Asp Glu 
                165                 170                 175     


Asn Gly Lys Glu Arg Thr Arg Glu Val Glu Tyr Leu Pro Thr Met Phe 
            180                 185                 190         


Arg His Cys Lys Glu Glu Ser Lys Tyr Lys Asp Ile Tyr Gly Lys Asn 
        195                 200                 205             


Cys Ala Pro Gln Lys Phe Pro Ser Met Lys Asp Ala Arg Asp Trp Met 
    210                 215                 220                 


Lys Arg Met Glu Asp Ile Gly Leu Glu Ala Leu Gly Met Asn Asp Phe 
225                 230                 235                 240 


Lys Leu Ala Tyr Ile Ser Asp Thr Tyr Gly Ser Glu Ile Val Tyr Asp 
                245                 250                 255     


Arg Lys Phe Val Arg Val Ala Asn Cys Asp Ile Glu Val Thr Gly Asp 
            260                 265                 270         


Lys Phe Pro Asp Pro Met Lys Ala Glu Tyr Glu Ile Asp Ala Ile Thr 
        275                 280                 285             


His Tyr Asp Ser Ile Asp Asp Arg Phe Tyr Val Phe Asp Leu Leu Asn 
    290                 295                 300                 


Ser Met Tyr Gly Ser Val Ser Lys Trp Asp Ala Lys Leu Ala Ala Lys 
305                 310                 315                 320 


Leu Asp Cys Glu Gly Gly Asp Glu Val Pro Gln Glu Ile Leu Asp Arg 
                325                 330                 335     


Val Ile Tyr Met Pro Phe Asp Asn Glu Arg Asp Met Leu Met Glu Tyr 
            340                 345                 350         


Ile Asn Leu Trp Glu Gln Lys Arg Pro Ala Ile Phe Thr Gly Trp Asn 
        355                 360                 365             


Ile Glu Gly Phe Asp Val Pro Tyr Ile Met Asn Arg Val Lys Met Ile 
    370                 375                 380                 


Leu Gly Glu Arg Ser Met Lys Arg Phe Ser Pro Ile Gly Arg Val Lys 
385                 390                 395                 400 


Ser Lys Leu Ile Gln Asn Met Tyr Gly Ser Lys Glu Ile Tyr Ser Ile 
                405                 410                 415     


Asp Gly Val Ser Ile Leu Asp Tyr Leu Asp Leu Tyr Lys Lys Phe Ala 
            420                 425                 430         


Phe Thr Asn Leu Pro Ser Phe Ser Leu Glu Ser Val Ala Gln His Glu 
        435                 440                 445             


Thr Lys Lys Gly Lys Leu Pro Tyr Asp Gly Pro Ile Asn Lys Leu Arg 
    450                 455                 460                 


Glu Thr Asn His Gln Arg Tyr Ile Ser Tyr Asn Ile Ile Asp Val Glu 
465                 470                 475                 480 


Ser Val Gln Ala Ile Asp Lys Ile Arg Gly Phe Ile Asp Leu Val Leu 
                485                 490                 495     


Ser Met Ser Tyr Tyr Ala Lys Met Pro Phe Ser Gly Val Met Ser Pro 
            500                 505                 510         


Ile Lys Thr Trp Asp Ala Ile Ile Phe Asn Ser Leu Lys Gly Glu His 
        515                 520                 525             


Lys Val Ile Pro Gln Gln Gly Ser His Val Lys Gln Ser Phe Pro Gly 
    530                 535                 540                 


Ala Phe Val Phe Glu Pro Lys Pro Ile Ala Arg Arg Tyr Ile Met Ser 
545                 550                 555                 560 


Phe Asp Leu Thr Ser Leu Tyr Pro Ser Ile Ile Arg Gln Val Asn Ile 
                565                 570                 575     


Ser Pro Glu Thr Ile Arg Gly Gln Phe Lys Val His Pro Ile His Glu 
            580                 585                 590         


Tyr Ile Ala Gly Thr Ala Pro Lys Pro Ser Asp Glu Tyr Ser Cys Ser 
        595                 600                 605             


Pro Asn Gly Trp Met Tyr Asp Lys His Gln Glu Gly Ile Ile Pro Lys 
    610                 615                 620                 


Glu Ile Ala Lys Val Phe Phe Gln Arg Lys Asp Trp Lys Lys Lys Met 
625                 630                 635                 640 


Phe Ala Glu Glu Met Asn Ala Glu Ala Ile Lys Lys Ile Ile Met Lys 
                645                 650                 655     


Gly Ala Gly Ser Cys Ser Thr Lys Pro Glu Val Glu Arg Tyr Val Lys 
            660                 665                 670         


Phe Ser Asp Asp Phe Leu Asn Glu Leu Ser Asn Tyr Thr Glu Ser Val 
        675                 680                 685             


Leu Asn Ser Leu Ile Glu Glu Cys Glu Lys Ala Ala Thr Leu Ala Asn 
    690                 695                 700                 


Thr Asn Gln Leu Asn Arg Lys Ile Leu Ile Asn Ser Leu Tyr Gly Ala 
705                 710                 715                 720 


Leu Gly Asn Ile His Phe Arg Tyr Tyr Asp Leu Arg Asn Ala Thr Ala 
                725                 730                 735     


Ile Thr Ile Phe Gly Gln Val Gly Ile Gln Trp Ile Ala Arg Lys Ile 
            740                 745                 750         


Asn Glu Tyr Leu Asn Lys Val Cys Gly Thr Asn Asp Glu Asp Phe Ile 
        755                 760                 765             


Ala Ala Gly Asp Thr Asp Ser Val Tyr Val Cys Val Asp Lys Val Ile 
    770                 775                 780                 


Glu Lys Val Gly Leu Asp Arg Phe Lys Glu Gln Asn Asp Leu Val Glu 
785                 790                 795                 800 


Phe Met Asn Gln Phe Gly Lys Lys Lys Met Glu Pro Met Ile Asp Val 
                805                 810                 815     


Ala Tyr Arg Glu Leu Cys Asp Tyr Met Asn Asn Arg Glu His Leu Met 
            820                 825                 830         


His Met Asp Arg Glu Ala Ile Ser Cys Pro Pro Leu Gly Ser Lys Gly 
        835                 840                 845             


Val Gly Gly Phe Trp Lys Ala Lys Lys Arg Tyr Ala Leu Asn Val Tyr 
    850                 855                 860                 


Asp Met Glu Asp Lys Arg Phe Ala Glu Pro His Leu Lys Ile Met Gly 
865                 870                 875                 880 


Met Glu Thr Gln Gln Ser Ser Thr Pro Lys Ala Val Gln Glu Ala Leu 
                885                 890                 895     


Glu Glu Ser Ile Arg Arg Ile Leu Gln Glu Gly Glu Glu Ser Val Gln 
            900                 905                 910         


Glu Tyr Tyr Lys Asn Phe Glu Lys Glu Tyr Arg Gln Leu Asp Tyr Lys 
        915                 920                 925             


Val Ile Ala Glu Val Lys Thr Ala Asn Asp Ile Ala Lys Tyr Asp Asp 
    930                 935                 940                 


Lys Gly Trp Pro Gly Phe Lys Cys Pro Phe His Ile Arg Gly Val Leu 
945                 950                 955                 960 


Thr Tyr Arg Arg Ala Val Ser Gly Leu Gly Val Ala Pro Ile Leu Asp 
                965                 970                 975     


Gly Asn Lys Val Met Val Leu Pro Leu Arg Glu Gly Asn Pro Phe Gly 
            980                 985                 990         


Asp Lys Cys Ile Ala Trp Pro Ser  Gly Thr Glu Leu Pro  Lys Glu Ile 
        995                 1000                 1005             


Arg Ser  Asp Val Leu Ser Trp  Ile Asp His Ser Thr  Leu Phe Gln 
    1010                 1015                 1020             


Lys Ser  Phe Val Lys Pro Leu  Ala Gly Met Cys Glu  Ser Ala Gly 
    1025                 1030                 1035             


Met Asp  Tyr Glu Glu Lys Ala  Ser Leu Asp Phe Leu  Phe Gly Gly 
    1040                 1045                 1050             


Ser Gly  Pro Lys Lys Lys Arg  Lys Val Ala Ala Ala  
    1055                 1060                 1065 


<210>  23
<211>  3186
<212>  DNA
<213>  artificial sequence

<220>
<223>  cDNA

<400>  23
atggcttcaa actttactca gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca       60

gtggctcctt ctaatttcgc taatggggtg gcagagtgga tcagctccaa ctcacggagc      120

caggcctaca aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc      180

atcaaggtgg aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc      240

gccgcttgga ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct      300

gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct      360

tccgccatcg ccgctaactc aggtatctac agcgctggag gaggtggaag cggaggagga      420

ggaagcggag gaggaggtag cggacctaag aaaaagagga aggtgaagga attctacatc      480

agcatcgaga ccgtgggtaa caacatcgtg gaaagatata ttgacgaaaa cggcaaggag      540

agaaccagag aggtggaata cctgcctaca atgttccggc actgtaaaga ggaatccaag      600

tacaaggata tctacggcaa aaactgcgcc cctcagaaat tccccagcat gaaagacgcc      660

agagattgga tgaagagaat ggaggatatc ggactggaag ccctgggcat gaacgatttc      720

aagctggcct acatctccga tacatacgga agcgagatcg tgtatgatag aaaattcgtg      780

cgggtggcca attgtgacat tgaggtgacc ggcgacaagt tccctgatcc catgaaagct      840

gaatatgaga tcgacgccat tacccactac gacagcatcg acgacagatt ctacgtgttc      900

gacctgctga actccatgta cggcagcgtg tccaagtggg acgctaagct ggccgccaag      960

ctggactgcg agggcggcga cgaggttcca caagagatcc tggaccgggt catctacatg     1020

cccttcgaca acgagaggga catgctgatg gaatacatca acctgtggga gcagaagcgc     1080

cccgccattt ttacaggctg gaacatcgag ggcttcgacg tgccttatat catgaataga     1140

gtgaaaatga tcctgggaga acggagcatg aaaagattca gccctatcgg cagagtgaag     1200

agcaagctga tccaaaacat gtacggctcc aaggaaatct atagcatcga tggcgtgtcc     1260

atcctggatt acctggacct gtacaaaaag ttcgccttca ccaacctgcc atctttctct     1320

cttgagagcg tcgcccagca cgagacaaag aagggcaagc tgccgtacga cggtcctatc     1380

aacaagctga gagaaacaaa tcaccagaga tacatcagct acaacatcat cgatgtggaa     1440

agcgttcagg ccatcgataa aatcagaggc ttcatcgacc tggtgctgtc tatgtcttac     1500

tacgccaaga tgccttttag cggagtgatg agccctatca agacctggga tgccatcatc     1560

ttcaacagcc tgaagggcga acacaaggtg atcccccaac agggcagcca cgtgaagcag     1620

agcttcccag gcgcttttgt gttcgagccc aagcccatag cgcggagata catcatgagc     1680

tttgatctga ccagcctgta ccccagcatc attcggcaag tgaacatttc tccagaaacc     1740

atcagaggcc agtttaaggt gcaccctatc cacgagtata ttgcaggcac cgctcctaaa     1800

cctagcgacg agtacagctg ctctcctaac ggctggatgt acgacaagca ccaggaggga     1860

atcatcccta aggaaattgc caaggtgttt ttccagcgga aggactggaa gaaaaaaatg     1920

ttcgccgagg aaatgaacgc cgaggccatc aagaagatca tcatgaaggg cgccggcagc     1980

tgctccacca agcctgaggt ggaaagatac gtgaagttca gcgacgattt cctgaatgag     2040

ctcagcaact acaccgagtc tgtcctgaac tcactgattg aggaatgcga gaaggccgcc     2100

accctggcta ataccaacca gctgaaccgg aagattctga tcaacagcct gtacggagct     2160

ctgggcaata ttcacttcag atactacgat ctgcgaaacg ccacagctat tacaattttc     2220

ggccaggtgg gcatccagtg gatcgccaga aagatcaatg agtacctgaa caaggtgtgc     2280

ggcaccaacg acgaggactt catcgccgct ggcgatactg atagcgtgta cgtttgtgtg     2340

gacaaggtca tcgagaaggt tggcctggac agatttaagg aacagaacga cctcgtggag     2400

ttcatgaacc agttcggaaa gaagaagatg gaacccatga tcgatgtggc ttatagagag     2460

ctgtgcgact acatgaacaa cagagagcac ctgatgcaca tggatagaga agctatttct     2520

tgccctcctc tgggctctaa gggagtgggc ggattttgga aagccaaaaa gagatacgcc     2580

ctgaatgtgt acgacatgga agataagaga ttcgccgagc ctcacctgaa aatcatgggc     2640

atggaaacac agcagagcag cacccctaag gctgtgcagg aggccctgga agagtctatc     2700

cggagaatct tgcaggaggg cgaggaaagc gtgcaggagt actacaagaa cttcgagaaa     2760

gaatacagac agctggacta caaggtgatc gcggaggtga agaccgctaa tgatatcgcc     2820

aagtacgacg acaagggctg gcccggcttc aagtgcccct tccacatcag aggcgtgctc     2880

acctaccgca gagccgtttc cggcctgggc gtggccccta tcctggatgg aaacaaagtc     2940

atggtgctgc ctctgagaga gggcaacccc tttggagata aatgcatcgc ttggcctagc     3000

ggcactgagc tgcccaagga aatccgctcc gacgtgctga gctggatcga tcacagcacc     3060

ctgttccaaa agtccttcgt gaagcccctg gccggcatgt gcgagtccgc cggcatggac     3120

tacgaggaaa aggccagcct ggatttcctg ttcggcggat ccggacctaa gaaaaagagg     3180

aaggtg                                                                3186


<210>  24
<211>  163
<212>  RNA
<213>  artificial sequence

<220>
<223>  MS2 binding sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<400>  24
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaggccaaca ugaggaucac ccaugucugc       60

agggccuagc aaguuaaaau aaggcuaguc cguuaucaac uuggccaaca ugaggaucac      120

ccaugucugc agggccaagu ggcaccgagu cggugcuuuu uuu                        163


<210>  25
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  25
agaguaacag ucugaguagg                                                   20


<210>  26
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  26
ccugcagggu ggccucaccu                                                   20


<210>  27
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  27
ggggccaggu ggccaaggug                                                   20


<210>  28
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  28
aaaauguaca aggaccgaca                                                   20


<210>  29
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  29
accagaguaa cagucugagu                                                   20


<210>  30
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  30
uauaaaauca cagaggguga                                                   20


<210>  31
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  31
caagcugaag gugaccaggg                                                   20


<210>  32
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  32
auuuauagcc caagauuucc                                                   20


<210>  33
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  33
gccugcuucc ucacagcuug                                                   20


<210>  34
<211>  20
<212>  RNA
<213>  artificial sequence

<220>
<223>  guide RNA

<400>  34
uucuugaacc aggaaaucuu                                                   20


