                         SEQUENCE LISTING

<110>  Intervet International BV
       Langereis, Martijn
       de Groof, Ad
       Vermeij, Paul
       Bosch, Berend-Jan
 
<120>  Recombinant Vectors Encoding Chimeric Coronavirus Spike Proteins 
       and Use Thereof

<130>  25143

<150>  EP20207302.9
<151>  2020-11-12

<150>  EP21166533.6
<151>  2021-04-01

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  3489
<212>  DNA
<213>  Infectious Bronchitis Virus

<400>  1
atgctggtga ccccactgct gctggtgaca ctgctgtgcg cactgtgctc cgccgctctg       60

tacgatagct ccagctacgt gtactactac cagagcgcat tccggccccc tgatggatgg      120

cacctgcacg gcggagccta cgctgtggtg aacatctcca gcgagagcaa caatgctggc      180

tccagctccg gatgcacagt ggggatcatt cacgggggca gagtggtgaa tgcaagctcc      240

attgcaatga ctgcccccag ctccggaatg gcatggagct ccagccagtt ttgcaccgcc      300

tactgcaact ttagcgatac cacagtgttc gtgacacact gctacaagca cggagggtgc      360

ccaatcactg gaatgctgca gcagcacagc attagggtgt ccgcaatgaa aaacgggcag      420

ctgttctaca atctgacagt gagcgtggcc aagtacccca cttttaaatc cttccagtgc      480

gtgaacaatc tgaccagcgt gtacctgaac ggcgatctgg tgtacaccag caatgctact      540

accgacgtga catccgcagg agtgtacttt aaggccggcg gacctatcac atacaaagtg      600

atgcgggaag tgagagcact ggcctacttc gtgaatggca ctgctcagga tgtgattctg      660

tgcgatggct ccccaagggg actgctggca tgccagtaca acaccggcaa tttcagcgac      720

ggattttacc ccttcacaaa ctccagcctg gtgaagcaga aatttatcgt gtaccgcgag      780

aacagcgtga atacaacttt cacactgcac aacttcactt ttcacaatga aaccggagcc      840

aaccccaatc ctagcggggt gcagaacatt cagacatacc agacccagac agctcagagc      900

gggtactaca acttcaattt ttccttcctg tccagctttg tgtacaagga gagcaacttc      960

atgtacggca gctaccaccc atcctgcaat tttcggctgg aaacaatcaa caatgggctg     1020

tggttcaact ccctgagcgt gtccattgct tacggccctc tgcagggcgg ctgcaaacag     1080

agcgtgtttt ccggaagagc cacctgctgc tacgcttact cctacggagg gccactgctg     1140

tgcaaggggg tgtacagcgg cgagctggat cacaatttcg aatgcggact gctggtgtac     1200

gtgaccaaaa gcggcggcag cagaatccag actgccaccg agccacccgt gatcacacag     1260

cacaactaca acaatattac actgaacact tgcgtggact acaatatcta cgggagaact     1320

ggccagggat tcattaccaa cgtgacagat agcgctgtgt cctacaatta cctggctgac     1380

gcaggcctgg caatcctgga taccagcggc agcatcgaca tttttgtggt gcagtccgag     1440

tacggcctga actactacaa agtgaatccc tgcgaagatg tgaaccagca gttcgttgtg     1500

agcgggggca aactggtggg aatcctgaca agccggaatg agactgggtc ccagctgctg     1560

gaaaaccagt tctacatcaa gatcactaac ggaaccagaa ggttccgccg gagcatcaca     1620

gagtccgtgg aaaactgccc ttacgtgtcc tacgggaagt tttgcattaa accagacggc     1680

agcatcgcca ctattgtgcc caagcagctg gagcagtttg tggctcctct gctgaacgtg     1740

accgaaaatg tgctgatccc aaacagcttc aatctgacag tgactgatga gtacattcag     1800

accaggatgg acaaagtgca gatcaactgc ctgcagtaca tttgcgggaa cagcctggaa     1860

tgccgcaatc tgttccagca gtacggccct gtgtgcgata acatcctgag cgtggtgaac     1920

agcgtgggcc agaaggagga catggaactg ctgaactttt actccagcac taaacccgcc     1980

ggcttcaaca cccctgtgct gagcaatgtg tccaccggag agtttaatat ctccctgttc     2040

ctgaccacac catccagccc cagaaggcgc agctttattg aggatctgct gttcacaagc     2100

gtggaatccg tggggctgcc cactgatgac gcttacaaga actgcaccgc aggccctctg     2160

ggattcctga aagacctggc ctgcgctcgc gagtacaatg gcctgctggt gctgcctcca     2220

atcattacag ctgaaatgca gatcctgtac acttccagcc tggtggctag catggcattt     2280

ggagggatca ctgcagccgg ggcaattccc ttcgccaccc agctgcaggc aaggatcaac     2340

cacctgggca ttacacagtc cctgctgctg aagaaccagg agaaaatcgc tgccagcttc     2400

aataaggcta ttgggcacat gcaggaaggc ttccgcagca cttccctggc actgcagcag     2460

atccaggatg tggtgaacaa gcagtccgcc attctgaccg agacaatggc tagcctgaac     2520

aaaaattttg gcgccatctc cagcgtgatc caggaaattt accagcagct ggatgctatc     2580

caggcaaacg cccaggtgga caggctgatt acaggacgcc tgtccagcct gagcgtgctg     2640

gcttccgcaa agcaggcaga gtacatccgg gtgtcccagc agagagagct ggccacacag     2700

aagatcaacg aatgcgtgaa aagccagtcc attcggtaca gcttctgcgg gaatggcaga     2760

cacgtgctga ctatccctca gaacgcccca aatggcatcg tgtttattca cttcagctac     2820

acccccgact cctttgtgaa cgtgacagct atcgtgggat tctgcgtgaa gccagccaat     2880

gcttcccagt acgctattgt gcctgcaaac ggaagaggga tctttattca agtgaatgga     2940

agctactaca tcactgcaag ggatatgtac atgcctcgcg ccatcaccgc tggggacatt     3000

gtgactctga ccagctgcca ggccaactac gtgtccgtga ataaaaccgt gatcactacc     3060

ttcgtggata acgatgactt tgatttcaat gacgagctga gcaagtggtg gaacgacaca     3120

aaacacgaac tgcctgattt tgacaagttc aattacactg tgccaatcct ggatattgac     3180

agcgagatcg ataggattca gggagtgatc caggggctga acgatagcct gattgacctg     3240

gaaaaactgt ccatcctgaa gacatacatt aaatggccct ggtacgtgtg gctggcaatc     3300

gcctttgcta ccatcatttt catcctgatt ctgggatggg tgttctttat gacagggtgc     3360

tgcggctgct gctgcggatg ctttgggatt atgcccctga tgagcaagtg cgggaagaaa     3420

tccagctact acacaacttt cgataacgac gtggtgaccg agcagtaccg ccctaagaaa     3480

agcgtgtga                                                             3489


<210>  2
<211>  1162
<212>  PRT
<213>  Infectious Bronchitis Virus

<400>  2

Met Leu Val Thr Pro Leu Leu Leu Val Thr Leu Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Ala Leu Tyr Asp Ser Ser Ser Tyr Val Tyr Tyr Tyr Gln Ser 
            20                  25                  30          


Ala Phe Arg Pro Pro Asp Gly Trp His Leu His Gly Gly Ala Tyr Ala 
        35                  40                  45              


Val Val Asn Ile Ser Ser Glu Ser Asn Asn Ala Gly Ser Ser Ser Gly 
    50                  55                  60                  


Cys Thr Val Gly Ile Ile His Gly Gly Arg Val Val Asn Ala Ser Ser 
65                  70                  75                  80  


Ile Ala Met Thr Ala Pro Ser Ser Gly Met Ala Trp Ser Ser Ser Gln 
                85                  90                  95      


Phe Cys Thr Ala Tyr Cys Asn Phe Ser Asp Thr Thr Val Phe Val Thr 
            100                 105                 110         


His Cys Tyr Lys His Gly Gly Cys Pro Ile Thr Gly Met Leu Gln Gln 
        115                 120                 125             


His Ser Ile Arg Val Ser Ala Met Lys Asn Gly Gln Leu Phe Tyr Asn 
    130                 135                 140                 


Leu Thr Val Ser Val Ala Lys Tyr Pro Thr Phe Lys Ser Phe Gln Cys 
145                 150                 155                 160 


Val Asn Asn Leu Thr Ser Val Tyr Leu Asn Gly Asp Leu Val Tyr Thr 
                165                 170                 175     


Ser Asn Ala Thr Thr Asp Val Thr Ser Ala Gly Val Tyr Phe Lys Ala 
            180                 185                 190         


Gly Gly Pro Ile Thr Tyr Lys Val Met Arg Glu Val Arg Ala Leu Ala 
        195                 200                 205             


Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Ile Leu Cys Asp Gly Ser 
    210                 215                 220                 


Pro Arg Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn Phe Ser Asp 
225                 230                 235                 240 


Gly Phe Tyr Pro Phe Thr Asn Ser Ser Leu Val Lys Gln Lys Phe Ile 
                245                 250                 255     


Val Tyr Arg Glu Asn Ser Val Asn Thr Thr Phe Thr Leu His Asn Phe 
            260                 265                 270         


Thr Phe His Asn Glu Thr Gly Ala Asn Pro Asn Pro Ser Gly Val Gln 
        275                 280                 285             


Asn Ile Gln Thr Tyr Gln Thr Gln Thr Ala Gln Ser Gly Tyr Tyr Asn 
    290                 295                 300                 


Phe Asn Phe Ser Phe Leu Ser Ser Phe Val Tyr Lys Glu Ser Asn Phe 
305                 310                 315                 320 


Met Tyr Gly Ser Tyr His Pro Ser Cys Asn Phe Arg Leu Glu Thr Ile 
                325                 330                 335     


Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Ile Ala Tyr Gly 
            340                 345                 350         


Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly Arg Ala Thr 
        355                 360                 365             


Cys Cys Tyr Ala Tyr Ser Tyr Gly Gly Pro Leu Leu Cys Lys Gly Val 
    370                 375                 380                 


Tyr Ser Gly Glu Leu Asp His Asn Phe Glu Cys Gly Leu Leu Val Tyr 
385                 390                 395                 400 


Val Thr Lys Ser Gly Gly Ser Arg Ile Gln Thr Ala Thr Glu Pro Pro 
                405                 410                 415     


Val Ile Thr Gln His Asn Tyr Asn Asn Ile Thr Leu Asn Thr Cys Val 
            420                 425                 430         


Asp Tyr Asn Ile Tyr Gly Arg Thr Gly Gln Gly Phe Ile Thr Asn Val 
        435                 440                 445             


Thr Asp Ser Ala Val Ser Tyr Asn Tyr Leu Ala Asp Ala Gly Leu Ala 
    450                 455                 460                 


Ile Leu Asp Thr Ser Gly Ser Ile Asp Ile Phe Val Val Gln Ser Glu 
465                 470                 475                 480 


Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp Val Asn Gln 
                485                 490                 495     


Gln Phe Val Val Ser Gly Gly Lys Leu Val Gly Ile Leu Thr Ser Arg 
            500                 505                 510         


Asn Glu Thr Gly Ser Gln Leu Leu Glu Asn Gln Phe Tyr Ile Lys Ile 
        515                 520                 525             


Thr Asn Gly Thr Arg Arg Phe Arg Arg Ser Ile Thr Glu Ser Val Glu 
    530                 535                 540                 


Asn Cys Pro Tyr Val Ser Tyr Gly Lys Phe Cys Ile Lys Pro Asp Gly 
545                 550                 555                 560 


Ser Ile Ala Thr Ile Val Pro Lys Gln Leu Glu Gln Phe Val Ala Pro 
                565                 570                 575     


Leu Leu Asn Val Thr Glu Asn Val Leu Ile Pro Asn Ser Phe Asn Leu 
            580                 585                 590         


Thr Val Thr Asp Glu Tyr Ile Gln Thr Arg Met Asp Lys Val Gln Ile 
        595                 600                 605             


Asn Cys Leu Gln Tyr Ile Cys Gly Asn Ser Leu Glu Cys Arg Asn Leu 
    610                 615                 620                 


Phe Gln Gln Tyr Gly Pro Val Cys Asp Asn Ile Leu Ser Val Val Asn 
625                 630                 635                 640 


Ser Val Gly Gln Lys Glu Asp Met Glu Leu Leu Asn Phe Tyr Ser Ser 
                645                 650                 655     


Thr Lys Pro Ala Gly Phe Asn Thr Pro Val Leu Ser Asn Val Ser Thr 
            660                 665                 670         


Gly Glu Phe Asn Ile Ser Leu Phe Leu Thr Thr Pro Ser Ser Pro Arg 
        675                 680                 685             


Arg Arg Ser Phe Ile Glu Asp Leu Leu Phe Thr Ser Val Glu Ser Val 
    690                 695                 700                 


Gly Leu Pro Thr Asp Asp Ala Tyr Lys Asn Cys Thr Ala Gly Pro Leu 
705                 710                 715                 720 


Gly Phe Leu Lys Asp Leu Ala Cys Ala Arg Glu Tyr Asn Gly Leu Leu 
                725                 730                 735     


Val Leu Pro Pro Ile Ile Thr Ala Glu Met Gln Ile Leu Tyr Thr Ser 
            740                 745                 750         


Ser Leu Val Ala Ser Met Ala Phe Gly Gly Ile Thr Ala Ala Gly Ala 
        755                 760                 765             


Ile Pro Phe Ala Thr Gln Leu Gln Ala Arg Ile Asn His Leu Gly Ile 
    770                 775                 780                 


Thr Gln Ser Leu Leu Leu Lys Asn Gln Glu Lys Ile Ala Ala Ser Phe 
785                 790                 795                 800 


Asn Lys Ala Ile Gly His Met Gln Glu Gly Phe Arg Ser Thr Ser Leu 
                805                 810                 815     


Ala Leu Gln Gln Ile Gln Asp Val Val Asn Lys Gln Ser Ala Ile Leu 
            820                 825                 830         


Thr Glu Thr Met Ala Ser Leu Asn Lys Asn Phe Gly Ala Ile Ser Ser 
        835                 840                 845             


Val Ile Gln Glu Ile Tyr Gln Gln Leu Asp Ala Ile Gln Ala Asn Ala 
    850                 855                 860                 


Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Ser Ser Leu Ser Val Leu 
865                 870                 875                 880 


Ala Ser Ala Lys Gln Ala Glu Tyr Ile Arg Val Ser Gln Gln Arg Glu 
                885                 890                 895     


Leu Ala Thr Gln Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Ile Arg 
            900                 905                 910         


Tyr Ser Phe Cys Gly Asn Gly Arg His Val Leu Thr Ile Pro Gln Asn 
        915                 920                 925             


Ala Pro Asn Gly Ile Val Phe Ile His Phe Ser Tyr Thr Pro Asp Ser 
    930                 935                 940                 


Phe Val Asn Val Thr Ala Ile Val Gly Phe Cys Val Lys Pro Ala Asn 
945                 950                 955                 960 


Ala Ser Gln Tyr Ala Ile Val Pro Ala Asn Gly Arg Gly Ile Phe Ile 
                965                 970                 975     


Gln Val Asn Gly Ser Tyr Tyr Ile Thr Ala Arg Asp Met Tyr Met Pro 
            980                 985                 990         


Arg Ala Ile Thr Ala Gly Asp Ile  Val Thr Leu Thr Ser  Cys Gln Ala 
        995                 1000                 1005             


Asn Tyr  Val Ser Val Asn Lys  Thr Val Ile Thr Thr  Phe Val Asp 
    1010                 1015                 1020             


Asn Asp  Asp Phe Asp Phe Asn  Asp Glu Leu Ser Lys  Trp Trp Asn 
    1025                 1030                 1035             


Asp Thr  Lys His Glu Leu Pro  Asp Phe Asp Lys Phe  Asn Tyr Thr 
    1040                 1045                 1050             


Val Pro  Ile Leu Asp Ile Asp  Ser Glu Ile Asp Arg  Ile Gln Gly 
    1055                 1060                 1065             


Val Ile  Gln Gly Leu Asn Asp  Ser Leu Ile Asp Leu  Glu Lys Leu 
    1070                 1075                 1080             


Ser Ile  Leu Lys Thr Tyr Ile  Lys Trp Pro Trp Tyr  Val Trp Leu 
    1085                 1090                 1095             


Ala Ile  Ala Phe Ala Thr Ile  Ile Phe Ile Leu Ile  Leu Gly Trp 
    1100                 1105                 1110             


Val Phe  Phe Met Thr Gly Cys  Cys Gly Cys Cys Cys  Gly Cys Phe 
    1115                 1120                 1125             


Gly Ile  Met Pro Leu Met Ser  Lys Cys Gly Lys Lys  Ser Ser Tyr 
    1130                 1135                 1140             


Tyr Thr  Thr Phe Asp Asn Asp  Val Val Thr Glu Gln  Tyr Arg Pro 
    1145                 1150                 1155             


Lys Lys  Ser Val 
    1160         


<210>  3
<211>  3423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified infectious bronchitis virus and 
       vesicular stomatitis virus

<400>  3
atgctggtga ccccactgct gctggtgaca ctgctgtgcg cactgtgctc cgccgctctg       60

tacgatagct ccagctacgt gtactactac cagagcgcat tccggccccc tgatggatgg      120

cacctgcacg gcggagccta cgctgtggtg aacatctcca gcgagagcaa caatgctggc      180

tccagctccg gatgcacagt ggggatcatt cacgggggca gagtggtgaa tgcaagctcc      240

attgcaatga ctgcccccag ctccggaatg gcatggagct ccagccagtt ttgcaccgcc      300

tactgcaact ttagcgatac cacagtgttc gtgacacact gctacaagca cggagggtgc      360

ccaatcactg gaatgctgca gcagcacagc attagggtgt ccgcaatgaa aaacgggcag      420

ctgttctaca atctgacagt gagcgtggcc aagtacccca cttttaaatc cttccagtgc      480

gtgaacaatc tgaccagcgt gtacctgaac ggcgatctgg tgtacaccag caatgctact      540

accgacgtga catccgcagg agtgtacttt aaggccggcg gacctatcac atacaaagtg      600

atgcgggaag tgagagcact ggcctacttc gtgaatggca ctgctcagga tgtgattctg      660

tgcgatggct ccccaagggg actgctggca tgccagtaca acaccggcaa tttcagcgac      720

ggattttacc ccttcacaaa ctccagcctg gtgaagcaga aatttatcgt gtaccgcgag      780

aacagcgtga atacaacttt cacactgcac aacttcactt ttcacaatga aaccggagcc      840

aaccccaatc ctagcggggt gcagaacatt cagacatacc agacccagac agctcagagc      900

gggtactaca acttcaattt ttccttcctg tccagctttg tgtacaagga gagcaacttc      960

atgtacggca gctaccaccc atcctgcaat tttcggctgg aaacaatcaa caatgggctg     1020

tggttcaact ccctgagcgt gtccattgct tacggccctc tgcagggcgg ctgcaaacag     1080

agcgtgtttt ccggaagagc cacctgctgc tacgcttact cctacggagg gccactgctg     1140

tgcaaggggg tgtacagcgg cgagctggat cacaatttcg aatgcggact gctggtgtac     1200

gtgaccaaaa gcggcggcag cagaatccag actgccaccg agccacccgt gatcacacag     1260

cacaactaca acaatattac actgaacact tgcgtggact acaatatcta cgggagaact     1320

ggccagggat tcattaccaa cgtgacagat agcgctgtgt cctacaatta cctggctgac     1380

gcaggcctgg caatcctgga taccagcggc agcatcgaca tttttgtggt gcagtccgag     1440

tacggcctga actactacaa agtgaatccc tgcgaagatg tgaaccagca gttcgttgtg     1500

agcgggggca aactggtggg aatcctgaca agccggaatg agactgggtc ccagctgctg     1560

gaaaaccagt tctacatcaa gatcactaac ggaaccgccg ccttcgccgc cagcatcaca     1620

gagtccgtgg aaaactgccc ttacgtgtcc tacgggaagt tttgcattaa accagacggc     1680

agcatcgcca ctattgtgcc caagcagctg gagcagtttg tggctcctct gctgaacgtg     1740

accgaaaatg tgctgatccc aaacagcttc aatctgacag tgactgatga gtacattcag     1800

accaggatgg acaaagtgca gatcaactgc ctgcagtaca tttgcgggaa cagcctggaa     1860

tgccgcaatc tgttccagca gtacggccct gtgtgcgata acatcctgag cgtggtgaac     1920

agcgtgggcc agaaggagga catggaactg ctgaactttt actccagcac taaacccgcc     1980

ggcttcaaca cccctgtgct gagcaatgtg tccaccggag agtttaatat ctccctgttc     2040

ctgaccacac catccagccc cagaaggcgc agctttattg aggatctgct gttcacaagc     2100

gtggaatccg tggggctgcc cactgatgac gcttacaaga actgcaccgc aggccctctg     2160

ggattcctga aagacctggc ctgcgctcgc gagtacaatg gcctgctggt gctgcctcca     2220

atcattacag ctgaaatgca gatcctgtac acttccagcc tggtggctag catggcattt     2280

ggagggatca ctgcagccgg ggcaattccc ttcgccaccc agctgcaggc aaggatcaac     2340

cacctgggca ttacacagtc cctgctgctg aagaaccagg agaaaatcgc tgccagcttc     2400

aataaggcta ttgggcacat gcaggaaggc ttccgcagca cttccctggc actgcagcag     2460

atccaggatg tggtgaacaa gcagtccgcc attctgaccg agacaatggc tagcctgaac     2520

aaaaattttg gcgccatctc cagcgtgatc caggaaattt accagcagct ggatgctatc     2580

caggcaaacg cccaggtgga caggctgatt acaggacgcc tgtccagcct gagcgtgctg     2640

gcttccgcaa agcaggcaga gtacatccgg gtgtcccagc agagagagct ggccacacag     2700

aagatcaacg aatgcgtgaa aagccagtcc attcggtaca gcttctgcgg gaatggcaga     2760

cacgtgctga ctatccctca gaacgcccca aatggcatcg tgtttattca cttcagctac     2820

acccccgact cctttgtgaa cgtgacagct atcgtgggat tctgcgtgaa gccagccaat     2880

gcttcccagt acgctattgt gcctgcaaac ggaagaggga tctttattca agtgaatgga     2940

agctactaca tcactgcaag ggatatgtac atgcctcgcg ccatcaccgc tggggacatt     3000

gtgactctga ccagctgcca ggccaactac gtgtccgtga ataaaaccgt gatcactacc     3060

ttcgtggata acgatgactt tgatttcaat gacgagctga gcaagtggtg gaacgacaca     3120

aaacacgaac tgcctgattt tgacaagttc aattacactg tgccaatcct ggatattgac     3180

agcgagatcg ataggattca gggagtgatc caggggctga acgatagcct gattgacctg     3240

gaaaaactgt ccatcctgaa gacatacatt aaatcctcca tcgcttcctt cttcttcatc     3300

atcggcctga tcatcggact gtttctggtg ctgagggtgg gcatctacct gtgcatcaag     3360

ctgaagcaca ctaagaagag gcagatctac accgacatcg agatgaacag gctgggcaag     3420

tga                                                                   3423


<210>  4
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified infectious bronchitis virus and 
       vesicular stomatitis virus

<400>  4

Met Leu Val Thr Pro Leu Leu Leu Val Thr Leu Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Ala Leu Tyr Asp Ser Ser Ser Tyr Val Tyr Tyr Tyr Gln Ser 
            20                  25                  30          


Ala Phe Arg Pro Pro Asp Gly Trp His Leu His Gly Gly Ala Tyr Ala 
        35                  40                  45              


Val Val Asn Ile Ser Ser Glu Ser Asn Asn Ala Gly Ser Ser Ser Gly 
    50                  55                  60                  


Cys Thr Val Gly Ile Ile His Gly Gly Arg Val Val Asn Ala Ser Ser 
65                  70                  75                  80  


Ile Ala Met Thr Ala Pro Ser Ser Gly Met Ala Trp Ser Ser Ser Gln 
                85                  90                  95      


Phe Cys Thr Ala Tyr Cys Asn Phe Ser Asp Thr Thr Val Phe Val Thr 
            100                 105                 110         


His Cys Tyr Lys His Gly Gly Cys Pro Ile Thr Gly Met Leu Gln Gln 
        115                 120                 125             


His Ser Ile Arg Val Ser Ala Met Lys Asn Gly Gln Leu Phe Tyr Asn 
    130                 135                 140                 


Leu Thr Val Ser Val Ala Lys Tyr Pro Thr Phe Lys Ser Phe Gln Cys 
145                 150                 155                 160 


Val Asn Asn Leu Thr Ser Val Tyr Leu Asn Gly Asp Leu Val Tyr Thr 
                165                 170                 175     


Ser Asn Ala Thr Thr Asp Val Thr Ser Ala Gly Val Tyr Phe Lys Ala 
            180                 185                 190         


Gly Gly Pro Ile Thr Tyr Lys Val Met Arg Glu Val Arg Ala Leu Ala 
        195                 200                 205             


Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Ile Leu Cys Asp Gly Ser 
    210                 215                 220                 


Pro Arg Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn Phe Ser Asp 
225                 230                 235                 240 


Gly Phe Tyr Pro Phe Thr Asn Ser Ser Leu Val Lys Gln Lys Phe Ile 
                245                 250                 255     


Val Tyr Arg Glu Asn Ser Val Asn Thr Thr Phe Thr Leu His Asn Phe 
            260                 265                 270         


Thr Phe His Asn Glu Thr Gly Ala Asn Pro Asn Pro Ser Gly Val Gln 
        275                 280                 285             


Asn Ile Gln Thr Tyr Gln Thr Gln Thr Ala Gln Ser Gly Tyr Tyr Asn 
    290                 295                 300                 


Phe Asn Phe Ser Phe Leu Ser Ser Phe Val Tyr Lys Glu Ser Asn Phe 
305                 310                 315                 320 


Met Tyr Gly Ser Tyr His Pro Ser Cys Asn Phe Arg Leu Glu Thr Ile 
                325                 330                 335     


Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Ile Ala Tyr Gly 
            340                 345                 350         


Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly Arg Ala Thr 
        355                 360                 365             


Cys Cys Tyr Ala Tyr Ser Tyr Gly Gly Pro Leu Leu Cys Lys Gly Val 
    370                 375                 380                 


Tyr Ser Gly Glu Leu Asp His Asn Phe Glu Cys Gly Leu Leu Val Tyr 
385                 390                 395                 400 


Val Thr Lys Ser Gly Gly Ser Arg Ile Gln Thr Ala Thr Glu Pro Pro 
                405                 410                 415     


Val Ile Thr Gln His Asn Tyr Asn Asn Ile Thr Leu Asn Thr Cys Val 
            420                 425                 430         


Asp Tyr Asn Ile Tyr Gly Arg Thr Gly Gln Gly Phe Ile Thr Asn Val 
        435                 440                 445             


Thr Asp Ser Ala Val Ser Tyr Asn Tyr Leu Ala Asp Ala Gly Leu Ala 
    450                 455                 460                 


Ile Leu Asp Thr Ser Gly Ser Ile Asp Ile Phe Val Val Gln Ser Glu 
465                 470                 475                 480 


Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp Val Asn Gln 
                485                 490                 495     


Gln Phe Val Val Ser Gly Gly Lys Leu Val Gly Ile Leu Thr Ser Arg 
            500                 505                 510         


Asn Glu Thr Gly Ser Gln Leu Leu Glu Asn Gln Phe Tyr Ile Lys Ile 
        515                 520                 525             


Thr Asn Gly Thr Ala Ala Phe Ala Ala Ser Ile Thr Glu Ser Val Glu 
    530                 535                 540                 


Asn Cys Pro Tyr Val Ser Tyr Gly Lys Phe Cys Ile Lys Pro Asp Gly 
545                 550                 555                 560 


Ser Ile Ala Thr Ile Val Pro Lys Gln Leu Glu Gln Phe Val Ala Pro 
                565                 570                 575     


Leu Leu Asn Val Thr Glu Asn Val Leu Ile Pro Asn Ser Phe Asn Leu 
            580                 585                 590         


Thr Val Thr Asp Glu Tyr Ile Gln Thr Arg Met Asp Lys Val Gln Ile 
        595                 600                 605             


Asn Cys Leu Gln Tyr Ile Cys Gly Asn Ser Leu Glu Cys Arg Asn Leu 
    610                 615                 620                 


Phe Gln Gln Tyr Gly Pro Val Cys Asp Asn Ile Leu Ser Val Val Asn 
625                 630                 635                 640 


Ser Val Gly Gln Lys Glu Asp Met Glu Leu Leu Asn Phe Tyr Ser Ser 
                645                 650                 655     


Thr Lys Pro Ala Gly Phe Asn Thr Pro Val Leu Ser Asn Val Ser Thr 
            660                 665                 670         


Gly Glu Phe Asn Ile Ser Leu Phe Leu Thr Thr Pro Ser Ser Pro Arg 
        675                 680                 685             


Arg Arg Ser Phe Ile Glu Asp Leu Leu Phe Thr Ser Val Glu Ser Val 
    690                 695                 700                 


Gly Leu Pro Thr Asp Asp Ala Tyr Lys Asn Cys Thr Ala Gly Pro Leu 
705                 710                 715                 720 


Gly Phe Leu Lys Asp Leu Ala Cys Ala Arg Glu Tyr Asn Gly Leu Leu 
                725                 730                 735     


Val Leu Pro Pro Ile Ile Thr Ala Glu Met Gln Ile Leu Tyr Thr Ser 
            740                 745                 750         


Ser Leu Val Ala Ser Met Ala Phe Gly Gly Ile Thr Ala Ala Gly Ala 
        755                 760                 765             


Ile Pro Phe Ala Thr Gln Leu Gln Ala Arg Ile Asn His Leu Gly Ile 
    770                 775                 780                 


Thr Gln Ser Leu Leu Leu Lys Asn Gln Glu Lys Ile Ala Ala Ser Phe 
785                 790                 795                 800 


Asn Lys Ala Ile Gly His Met Gln Glu Gly Phe Arg Ser Thr Ser Leu 
                805                 810                 815     


Ala Leu Gln Gln Ile Gln Asp Val Val Asn Lys Gln Ser Ala Ile Leu 
            820                 825                 830         


Thr Glu Thr Met Ala Ser Leu Asn Lys Asn Phe Gly Ala Ile Ser Ser 
        835                 840                 845             


Val Ile Gln Glu Ile Tyr Gln Gln Leu Asp Ala Ile Gln Ala Asn Ala 
    850                 855                 860                 


Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Ser Ser Leu Ser Val Leu 
865                 870                 875                 880 


Ala Ser Ala Lys Gln Ala Glu Tyr Ile Arg Val Ser Gln Gln Arg Glu 
                885                 890                 895     


Leu Ala Thr Gln Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Ile Arg 
            900                 905                 910         


Tyr Ser Phe Cys Gly Asn Gly Arg His Val Leu Thr Ile Pro Gln Asn 
        915                 920                 925             


Ala Pro Asn Gly Ile Val Phe Ile His Phe Ser Tyr Thr Pro Asp Ser 
    930                 935                 940                 


Phe Val Asn Val Thr Ala Ile Val Gly Phe Cys Val Lys Pro Ala Asn 
945                 950                 955                 960 


Ala Ser Gln Tyr Ala Ile Val Pro Ala Asn Gly Arg Gly Ile Phe Ile 
                965                 970                 975     


Gln Val Asn Gly Ser Tyr Tyr Ile Thr Ala Arg Asp Met Tyr Met Pro 
            980                 985                 990         


Arg Ala Ile Thr Ala Gly Asp Ile  Val Thr Leu Thr Ser  Cys Gln Ala 
        995                 1000                 1005             


Asn Tyr  Val Ser Val Asn Lys  Thr Val Ile Thr Thr  Phe Val Asp 
    1010                 1015                 1020             


Asn Asp  Asp Phe Asp Phe Asn  Asp Glu Leu Ser Lys  Trp Trp Asn 
    1025                 1030                 1035             


Asp Thr  Lys His Glu Leu Pro  Asp Phe Asp Lys Phe  Asn Tyr Thr 
    1040                 1045                 1050             


Val Pro  Ile Leu Asp Ile Asp  Ser Glu Ile Asp Arg  Ile Gln Gly 
    1055                 1060                 1065             


Val Ile  Gln Gly Leu Asn Asp  Ser Leu Ile Asp Leu  Glu Lys Leu 
    1070                 1075                 1080             


Ser Ile  Leu Lys Thr Tyr Ile  Lys Ser Ser Ile Ala  Ser Phe Phe 
    1085                 1090                 1095             


Phe Ile  Ile Gly Leu Ile Ile  Gly Leu Phe Leu Val  Leu Arg Val 
    1100                 1105                 1110             


Gly Ile  Tyr Leu Cys Ile Lys  Leu Lys His Thr Lys  Lys Arg Gln 
    1115                 1120                 1125             


Ile Tyr  Thr Asp Ile Glu Met  Asn Arg Leu Gly Lys  
    1130                 1135                 1140 


<210>  5
<211>  3423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified infectious bronchitis virus and 
       vesicular stomatitis virus

<400>  5
atgctggtga ccccactgct gctggtgaca ctgctgtgcg cactgtgctc cgccgctctg       60

tacgatagct ccagctacgt gtactactac cagagcgcat tccggccccc tgatggatgg      120

cacctgcacg gcggagccta cgctgtggtg aacatctcca gcgagagcaa caatgctggc      180

tccagctccg gatgcacagt ggggatcatt cacgggggca gagtggtgaa tgcaagctcc      240

attgcaatga ctgcccccag ctccggaatg gcatggagct ccagccagtt ttgcaccgcc      300

tactgcaact ttagcgatac cacagtgttc gtgacacact gctacaagca cggagggtgc      360

ccaatcactg gaatgctgca gcagcacagc attagggtgt ccgcaatgaa aaacgggcag      420

ctgttctaca atctgacagt gagcgtggcc aagtacccca cttttaaatc cttccagtgc      480

gtgaacaatc tgaccagcgt gtacctgaac ggcgatctgg tgtacaccag caatgctact      540

accgacgtga catccgcagg agtgtacttt aaggccggcg gacctatcac atacaaagtg      600

atgcgggaag tgagagcact ggcctacttc gtgaatggca ctgctcagga tgtgattctg      660

tgcgatggct ccccaagggg actgctggca tgccagtaca acaccggcaa tttcagcgac      720

ggattttacc ccttcacaaa ctccagcctg gtgaagcaga aatttatcgt gtaccgcgag      780

aacagcgtga atacaacttt cacactgcac aacttcactt ttcacaatga aaccggagcc      840

aaccccaatc ctagcggggt gcagaacatt cagacatacc agacccagac agctcagagc      900

gggtactaca acttcaattt ttccttcctg tccagctttg tgtacaagga gagcaacttc      960

atgtacggca gctaccaccc atcctgcaat tttcggctgg aaacaatcaa caatgggctg     1020

tggttcaact ccctgagcgt gtccattgct tacggccctc tgcagggcgg ctgcaaacag     1080

agcgtgtttt ccggaagagc cacctgctgc tacgcttact cctacggagg gccactgctg     1140

tgcaaggggg tgtacagcgg cgagctggat cacaatttcg aatgcggact gctggtgtac     1200

gtgaccaaaa gcggcggcag cagaatccag actgccaccg agccacccgt gatcacacag     1260

cacaactaca acaatattac actgaacact tgcgtggact acaatatcta cgggagaact     1320

ggccagggat tcattaccaa cgtgacagat agcgctgtgt cctacaatta cctggctgac     1380

gcaggcctgg caatcctgga taccagcggc agcatcgaca tttttgtggt gcagtccgag     1440

tacggcctga actactacaa agtgaatccc tgcgaagatg tgaaccagca gttcgttgtg     1500

agcgggggca aactggtggg aatcctgaca agccggaatg agactgggtc ccagctgctg     1560

gaaaaccagt tctacatcaa gatcactaac ggaaccgccg ccttcgccgc cagcatcaca     1620

gagtccgtgg aaaactgccc ttacgtgtcc tacgggaagt tttgcattaa accagacggc     1680

agcatcgcca ctattgtgcc caagcagctg gagcagtttg tggctcctct gctgaacgtg     1740

accgaaaatg tgctgatccc aaacagcttc aatctgacag tgactgatga gtacattcag     1800

accaggatgg acaaagtgca gatcaactgc ctgcagtaca tttgcgggaa cagcctggaa     1860

tgccgcaatc tgttccagca gtacggccct gtgtgcgata acatcctgag cgtggtgaac     1920

agcgtgggcc agaaggagga catggaactg ctgaactttt actccagcac taaacccgcc     1980

ggcttcaaca cccctgtgct gagcaatgtg tccaccggag agtttaatat ctccctgttc     2040

ctgaccacac catccagccc cagaaggcgc agctttattg aggatctgct gttcacaagc     2100

gtggaatccg tggggctgcc cactgatgac gcttacaaga actgcaccgc aggccctctg     2160

ggattcctga aagacctggc ctgcgctcgc gagtacaatg gcctgctggt gctgcctcca     2220

atcattacag ctgaaatgca gatcctgtac acttccagcc tggtggctag catggcattt     2280

ggagggatca ctgcagccgg ggcaattccc ttcgccaccc agctgcaggc aaggatcaac     2340

cacctgggca ttacacagtc cctgctgctg aagaaccagg agaaaatcgc tgccagcttc     2400

aataaggcta ttgggcacat gcaggaaggc ttccgcagca cttccctggc actgcagcag     2460

atccaggatg tggtgaacaa gcagtccgcc attctgaccg agacaatggc tagcctgaac     2520

aaaaattttg gcgccatctc cagcgtgatc caggaaattt accagcagct ggatcccccc     2580

caggcaaacg cccaggtgga caggctgatt acaggacgcc tgtccagcct gagcgtgctg     2640

gcttccgcaa agcaggcaga gtacatccgg gtgtcccagc agagagagct ggccacacag     2700

aagatcaacg aatgcgtgaa aagccagtcc attcggtaca gcttctgcgg gaatggcaga     2760

cacgtgctga ctatccctca gaacgcccca aatggcatcg tgtttattca cttcagctac     2820

acccccgact cctttgtgaa cgtgacagct atcgtgggat tctgcgtgaa gccagccaat     2880

gcttcccagt acgctattgt gcctgcaaac ggaagaggga tctttattca agtgaatgga     2940

agctactaca tcactgcaag ggatatgtac atgcctcgcg ccatcaccgc tggggacatt     3000

gtgactctga ccagctgcca ggccaactac gtgtccgtga ataaaaccgt gatcactacc     3060

ttcgtggata acgatgactt tgatttcaat gacgagctga gcaagtggtg gaacgacaca     3120

aaacacgaac tgcctgattt tgacaagttc aattacactg tgccaatcct ggatattgac     3180

agcgagatcg ataggattca gggagtgatc caggggctga acgatagcct gattgacctg     3240

gaaaaactgt ccatcctgaa gacatacatt aaatcctcca tcgcttcctt cttcttcatc     3300

atcggcctga tcatcggact gtttctggtg ctgagggtgg gcatctacct gtgcatcaag     3360

ctgaagcaca ctaagaagag gcagatctac accgacatcg agatgaacag gctgggcaag     3420

tga                                                                   3423


<210>  6
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified infectious bronchitis virus and 
       vesicular stomatitis virus

<400>  6

Met Leu Val Thr Pro Leu Leu Leu Val Thr Leu Leu Cys Ala Leu Cys 
1               5                   10                  15      


Ser Ala Ala Leu Tyr Asp Ser Ser Ser Tyr Val Tyr Tyr Tyr Gln Ser 
            20                  25                  30          


Ala Phe Arg Pro Pro Asp Gly Trp His Leu His Gly Gly Ala Tyr Ala 
        35                  40                  45              


Val Val Asn Ile Ser Ser Glu Ser Asn Asn Ala Gly Ser Ser Ser Gly 
    50                  55                  60                  


Cys Thr Val Gly Ile Ile His Gly Gly Arg Val Val Asn Ala Ser Ser 
65                  70                  75                  80  


Ile Ala Met Thr Ala Pro Ser Ser Gly Met Ala Trp Ser Ser Ser Gln 
                85                  90                  95      


Phe Cys Thr Ala Tyr Cys Asn Phe Ser Asp Thr Thr Val Phe Val Thr 
            100                 105                 110         


His Cys Tyr Lys His Gly Gly Cys Pro Ile Thr Gly Met Leu Gln Gln 
        115                 120                 125             


His Ser Ile Arg Val Ser Ala Met Lys Asn Gly Gln Leu Phe Tyr Asn 
    130                 135                 140                 


Leu Thr Val Ser Val Ala Lys Tyr Pro Thr Phe Lys Ser Phe Gln Cys 
145                 150                 155                 160 


Val Asn Asn Leu Thr Ser Val Tyr Leu Asn Gly Asp Leu Val Tyr Thr 
                165                 170                 175     


Ser Asn Ala Thr Thr Asp Val Thr Ser Ala Gly Val Tyr Phe Lys Ala 
            180                 185                 190         


Gly Gly Pro Ile Thr Tyr Lys Val Met Arg Glu Val Arg Ala Leu Ala 
        195                 200                 205             


Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Ile Leu Cys Asp Gly Ser 
    210                 215                 220                 


Pro Arg Gly Leu Leu Ala Cys Gln Tyr Asn Thr Gly Asn Phe Ser Asp 
225                 230                 235                 240 


Gly Phe Tyr Pro Phe Thr Asn Ser Ser Leu Val Lys Gln Lys Phe Ile 
                245                 250                 255     


Val Tyr Arg Glu Asn Ser Val Asn Thr Thr Phe Thr Leu His Asn Phe 
            260                 265                 270         


Thr Phe His Asn Glu Thr Gly Ala Asn Pro Asn Pro Ser Gly Val Gln 
        275                 280                 285             


Asn Ile Gln Thr Tyr Gln Thr Gln Thr Ala Gln Ser Gly Tyr Tyr Asn 
    290                 295                 300                 


Phe Asn Phe Ser Phe Leu Ser Ser Phe Val Tyr Lys Glu Ser Asn Phe 
305                 310                 315                 320 


Met Tyr Gly Ser Tyr His Pro Ser Cys Asn Phe Arg Leu Glu Thr Ile 
                325                 330                 335     


Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Ile Ala Tyr Gly 
            340                 345                 350         


Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe Ser Gly Arg Ala Thr 
        355                 360                 365             


Cys Cys Tyr Ala Tyr Ser Tyr Gly Gly Pro Leu Leu Cys Lys Gly Val 
    370                 375                 380                 


Tyr Ser Gly Glu Leu Asp His Asn Phe Glu Cys Gly Leu Leu Val Tyr 
385                 390                 395                 400 


Val Thr Lys Ser Gly Gly Ser Arg Ile Gln Thr Ala Thr Glu Pro Pro 
                405                 410                 415     


Val Ile Thr Gln His Asn Tyr Asn Asn Ile Thr Leu Asn Thr Cys Val 
            420                 425                 430         


Asp Tyr Asn Ile Tyr Gly Arg Thr Gly Gln Gly Phe Ile Thr Asn Val 
        435                 440                 445             


Thr Asp Ser Ala Val Ser Tyr Asn Tyr Leu Ala Asp Ala Gly Leu Ala 
    450                 455                 460                 


Ile Leu Asp Thr Ser Gly Ser Ile Asp Ile Phe Val Val Gln Ser Glu 
465                 470                 475                 480 


Tyr Gly Leu Asn Tyr Tyr Lys Val Asn Pro Cys Glu Asp Val Asn Gln 
                485                 490                 495     


Gln Phe Val Val Ser Gly Gly Lys Leu Val Gly Ile Leu Thr Ser Arg 
            500                 505                 510         


Asn Glu Thr Gly Ser Gln Leu Leu Glu Asn Gln Phe Tyr Ile Lys Ile 
        515                 520                 525             


Thr Asn Gly Thr Ala Ala Phe Ala Ala Ser Ile Thr Glu Ser Val Glu 
    530                 535                 540                 


Asn Cys Pro Tyr Val Ser Tyr Gly Lys Phe Cys Ile Lys Pro Asp Gly 
545                 550                 555                 560 


Ser Ile Ala Thr Ile Val Pro Lys Gln Leu Glu Gln Phe Val Ala Pro 
                565                 570                 575     


Leu Leu Asn Val Thr Glu Asn Val Leu Ile Pro Asn Ser Phe Asn Leu 
            580                 585                 590         


Thr Val Thr Asp Glu Tyr Ile Gln Thr Arg Met Asp Lys Val Gln Ile 
        595                 600                 605             


Asn Cys Leu Gln Tyr Ile Cys Gly Asn Ser Leu Glu Cys Arg Asn Leu 
    610                 615                 620                 


Phe Gln Gln Tyr Gly Pro Val Cys Asp Asn Ile Leu Ser Val Val Asn 
625                 630                 635                 640 


Ser Val Gly Gln Lys Glu Asp Met Glu Leu Leu Asn Phe Tyr Ser Ser 
                645                 650                 655     


Thr Lys Pro Ala Gly Phe Asn Thr Pro Val Leu Ser Asn Val Ser Thr 
            660                 665                 670         


Gly Glu Phe Asn Ile Ser Leu Phe Leu Thr Thr Pro Ser Ser Pro Arg 
        675                 680                 685             


Arg Arg Ser Phe Ile Glu Asp Leu Leu Phe Thr Ser Val Glu Ser Val 
    690                 695                 700                 


Gly Leu Pro Thr Asp Asp Ala Tyr Lys Asn Cys Thr Ala Gly Pro Leu 
705                 710                 715                 720 


Gly Phe Leu Lys Asp Leu Ala Cys Ala Arg Glu Tyr Asn Gly Leu Leu 
                725                 730                 735     


Val Leu Pro Pro Ile Ile Thr Ala Glu Met Gln Ile Leu Tyr Thr Ser 
            740                 745                 750         


Ser Leu Val Ala Ser Met Ala Phe Gly Gly Ile Thr Ala Ala Gly Ala 
        755                 760                 765             


Ile Pro Phe Ala Thr Gln Leu Gln Ala Arg Ile Asn His Leu Gly Ile 
    770                 775                 780                 


Thr Gln Ser Leu Leu Leu Lys Asn Gln Glu Lys Ile Ala Ala Ser Phe 
785                 790                 795                 800 


Asn Lys Ala Ile Gly His Met Gln Glu Gly Phe Arg Ser Thr Ser Leu 
                805                 810                 815     


Ala Leu Gln Gln Ile Gln Asp Val Val Asn Lys Gln Ser Ala Ile Leu 
            820                 825                 830         


Thr Glu Thr Met Ala Ser Leu Asn Lys Asn Phe Gly Ala Ile Ser Ser 
        835                 840                 845             


Val Ile Gln Glu Ile Tyr Gln Gln Leu Asp Pro Pro Gln Ala Asn Ala 
    850                 855                 860                 


Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Ser Ser Leu Ser Val Leu 
865                 870                 875                 880 


Ala Ser Ala Lys Gln Ala Glu Tyr Ile Arg Val Ser Gln Gln Arg Glu 
                885                 890                 895     


Leu Ala Thr Gln Lys Ile Asn Glu Cys Val Lys Ser Gln Ser Ile Arg 
            900                 905                 910         


Tyr Ser Phe Cys Gly Asn Gly Arg His Val Leu Thr Ile Pro Gln Asn 
        915                 920                 925             


Ala Pro Asn Gly Ile Val Phe Ile His Phe Ser Tyr Thr Pro Asp Ser 
    930                 935                 940                 


Phe Val Asn Val Thr Ala Ile Val Gly Phe Cys Val Lys Pro Ala Asn 
945                 950                 955                 960 


Ala Ser Gln Tyr Ala Ile Val Pro Ala Asn Gly Arg Gly Ile Phe Ile 
                965                 970                 975     


Gln Val Asn Gly Ser Tyr Tyr Ile Thr Ala Arg Asp Met Tyr Met Pro 
            980                 985                 990         


Arg Ala Ile Thr Ala Gly Asp Ile  Val Thr Leu Thr Ser  Cys Gln Ala 
        995                 1000                 1005             


Asn Tyr  Val Ser Val Asn Lys  Thr Val Ile Thr Thr  Phe Val Asp 
    1010                 1015                 1020             


Asn Asp  Asp Phe Asp Phe Asn  Asp Glu Leu Ser Lys  Trp Trp Asn 
    1025                 1030                 1035             


Asp Thr  Lys His Glu Leu Pro  Asp Phe Asp Lys Phe  Asn Tyr Thr 
    1040                 1045                 1050             


Val Pro  Ile Leu Asp Ile Asp  Ser Glu Ile Asp Arg  Ile Gln Gly 
    1055                 1060                 1065             


Val Ile  Gln Gly Leu Asn Asp  Ser Leu Ile Asp Leu  Glu Lys Leu 
    1070                 1075                 1080             


Ser Ile  Leu Lys Thr Tyr Ile  Lys Ser Ser Ile Ala  Ser Phe Phe 
    1085                 1090                 1095             


Phe Ile  Ile Gly Leu Ile Ile  Gly Leu Phe Leu Val  Leu Arg Val 
    1100                 1105                 1110             


Gly Ile  Tyr Leu Cys Ile Lys  Leu Lys His Thr Lys  Lys Arg Gln 
    1115                 1120                 1125             


Ile Tyr  Thr Asp Ile Glu Met  Asn Arg Leu Gly Lys  
    1130                 1135                 1140 


<210>  7
<211>  3822
<212>  DNA
<213>  Severe Acute Respiratory Syndrome Coronavirus 2

<400>  7
atgttcgtgt tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccacc       60

aggacccagc tgccaccagc ctacaccaac agcttcacca ggggcgtgta ctaccccgac      120

aaagtgttca gatcttccgt gctgcacagc acccaggacc tgttcctgcc cttcttctct      180

aacgtgacct ggttccacgc catccacgtg tccggcacca acggcaccaa gaggttcgac      240

aaccccgtgc tgcccttcaa cgacggcgtg tacttcgcca gcaccgagaa gtctaacatc      300

atcagaggct ggatcttcgg caccaccctg gactccaaaa cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttctgtaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaaa tcttggatgg agtccgagtt cagggtgtac      480

agctctgcca acaactgcac cttcgagtac gtgagccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa aaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaaaca cacccccatc aacctggtgc gcgacctgcc acagggcttc      660

tctgccctgg agccactggt ggacctgcca atcggcatca acatcaccag gttccagacc      720

ctgctggccc tgcacagatc ctacctgacc ccaggcgact ccagctctgg atggaccgct      780

ggagctgccg cctactacgt gggctacctg cagccccgga ccttcctgct gaaatacaac      840

gagaacggaa ccatcaccga cgctgtggac tgcgctctgg acccactgtc tgaaaccaag      900

tgtaccctga aatccttcac cgtggagaag ggcatctacc agacctccaa cttccgggtg      960

cagcccaccg aaagcatcgt gcgcttcccc aacatcacca acctgtgccc cttcggcgag     1020

gtgttcaacg ctaccaggtt cgctagcgtg tacgcttgga accggaagcg catcagcaac     1080

tgcgtggccg actactctgt gctgtacaac tccgccagct tctctacctt caagtgctac     1140

ggcgtgtccc ccaccaaact gaacgacctg tgcttcacca acgtgtacgc cgacagcttc     1200

gtgatcaggg gcgacgaggt gcgccagatc gctccaggac agaccggcaa gatcgctgac     1260

tacaactaca aactgcccga cgacttcacc ggctgcgtga tcgcctggaa ctctaacaac     1320

ctggactcca aagtgggcgg caactacaac tacctgtaca ggctgttcag aaagtctaac     1380

ctgaaaccct tcgagcggga catcagcacc gaaatctacc aggctggatc taccccatgc     1440

aacggagtgg agggcttcaa ctgttacttc cccctgcagt cctacggctt ccagccaacc     1500

aacggagtgg gataccagcc atacagggtg gtggtgctgt ctttcgaact gctgcacgct     1560

ccagctaccg tgtgcggacc caagaaatcc accaacctgg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggactgac cggaaccggc gtgctgaccg agagcaacaa gaaattcctg     1680

cccttccagc agttcggccg ggacatcgct gacaccaccg acgccgtgcg cgacccccag     1740

accctggaaa tcctggacat caccccctgc agcttcggcg gcgtgtctgt gatcacccca     1800

ggaaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg taccgaggtg     1860

ccagtggcta tccacgctga ccagctgacc ccaacctgga gggtgtactc taccggctcc     1920

aacgtgttcc agaccagagc tggatgcctg atcggagctg agcacgtgaa caactcctac     1980

gaatgcgaca tccccatcgg cgccggcatc tgtgccagct accagaccca gaccaacagc     2040

ccaaggagag ccaggtctgt ggcttcccag agcatcatcg cctacaccat gtccctgggc     2100

gccgaaaaca gcgtggccta cagcaacaac tctatcgcca tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gcccgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgtg gcgacagcac cgaatgctct aacctgctgc tgcagtacgg ctccttctgt     2280

acccagctga acagagccct gaccggaatc gctgtggagc aggacaaaaa cacccaggaa     2340

gtgttcgccc aggtgaagca gatctacaaa acccccccca tcaaggactt cggcggcttc     2400

aacttctccc agatcctgcc cgacccctcc aagcccagca aaaggtcttt catcgaggac     2460

ctgctgttca acaaggtgac cctggccgac gccggcttca tcaaacagta cggcgactgc     2520

ctgggcgaca tcgctgctag agacctgatc tgtgcccaga agttcaacgg actgaccgtg     2580

ctgccaccac tgctgaccga cgaaatgatc gctcagtaca cctctgccct gctggctgga     2640

accatcacct ccggatggac cttcggcgct ggagccgccc tgcagatccc cttcgccatg     2700

cagatggcct acagattcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaaa tccaggactc tctgtccagc     2820

accgcttccg ccctgggcaa actgcaggac gtggtgaacc agaacgccca ggccctgaac     2880

accctggtga agcagctgtc ttccaacttc ggcgccatca gctctgtgct gaacgacatc     2940

ctgtccaggc tggacaaagt ggaggccgaa gtgcagatcg acaggctgat caccggcaga     3000

ctgcagagcc tgcagaccta cgtgacccag cagctgatca gggctgctga aatcagggct     3060

tctgccaacc tggctgctac caagatgtcc gagtgcgtgc tgggccagag caagagagtg     3120

gacttctgtg gcaaaggcta ccacctgatg tccttcccac agagcgcccc acacggagtg     3180

gtgttcctgc acgtgaccta cgtgcccgcc caggagaaga acttcaccac cgctccagct     3240

atctgccacg acggcaaagc tcacttccca agggaaggcg tgttcgtgtc caacggcacc     3300

cactggttcg tgacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgagcg gcaactgtga cgtggtcatc ggaatcgtga acaacaccgt gtacgaccca     3420

ctgcagccag agctggactc tttcaaggag gaactggaca agtacttcaa aaaccacacc     3480

tccccagacg tggacctggg cgacatctct ggcatcaacg cctccgtggt gaacatccag     3540

aaggagatcg acaggctgaa cgaagtggcc aaaaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aaatggccct ggtacatctg gctgggcttc     3660

atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacctcctgc     3720

tgtagctgcc tgaagggctg ctgttcttgt ggctcctgct gtaaattcga cgaggacgac     3780

tccgaacccg tgctgaaggg cgtgaaactg cactacacct ga                        3822


<210>  8
<211>  1273
<212>  PRT
<213>  Severe Acute Respiratory Syndrome Coronavirus 2

<400>  8

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  9
<211>  3783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified Severe Acute Respiratory Syndrome 
       Coronavirus 2 and vesicular stomatitis virus

<400>  9
atgttcgtgt tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccacc       60

agaacccagc tgccaccagc ctacaccaac agcttcaccc ggggcgtgta ctaccccgac      120

aaagtgttcc gctcttccgt gctgcactct acccaggacc tgttcctgcc cttcttctcc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca acggcaccaa gaggttcgac      240

aaccccgtgc tgcccttcaa cgacggcgtg tacttcgcct ctaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccaccctg gacagcaaaa cccagtctct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttctgtaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaaa tcctggatgg agagcgagtt cagggtgtac      480

agctctgcca acaactgtac cttcgagtac gtgagccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa aaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actctaaaca cacccccatc aacctggtgc gcgacctgcc acagggcttc      660

tccgccctgg agccactggt ggacctgccc atcggcatca acatcaccag gttccagacc      720

ctgctggccc tgcaccgctc ctacctgacc ccaggcgact ccagctctgg atggaccgct      780

ggagctgccg cctactacgt gggctacctg cagcccagga ccttcctgct gaaatacaac      840

gaaaacggaa ccatcaccga cgctgtggac tgcgctctgg acccactgtc cgaaaccaag      900

tgtaccctga aaagcttcac cgtggagaag ggcatctacc agaccagcaa cttcagggtg      960

cagcccaccg aatctatcgt gagattcccc aacatcacca acctgtgccc cttcggcgag     1020

gtgttcaacg ccaccagatt cgccagcgtg tacgcctgga acaggaagag aatctctaac     1080

tgcgtggccg actactccgt gctgtacaac tctgcctcct tcagcacctt caagtgctac     1140

ggcgtgagcc ccaccaaact gaacgacctg tgcttcacca acgtgtacgc cgactctttc     1200

gtgatcaggg gcgacgaggt gagacagatc gctccaggac agaccggcaa gatcgctgac     1260

tacaactaca aactgcccga cgacttcacc ggctgcgtga tcgcctggaa ctccaacaac     1320

ctggacagca aagtgggcgg caactacaac tacctgtacc ggctgttccg caagagcaac     1380

ctgaaaccct tcgagcggga catctctacc gaaatctacc aggctggatc caccccatgc     1440

aacggagtgg agggcttcaa ctgttacttc cccctgcagt cctacggctt ccagccaacc     1500

aacggagtgg gataccagcc atacagggtg gtggtgctgt ccttcgaact gctgcacgct     1560

ccagctaccg tgtgcggacc caagaaaagc accaacctgg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggactgac cggaaccggc gtgctgaccg agagcaacaa gaaattcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccaccg acgccgtgag agacccacag     1740

accctggaaa tcctggacat caccccctgc tctttcggcg gcgtgtccgt gatcacccca     1800

ggaaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg taccgaggtg     1860

ccagtggcta tccacgctga ccagctgacc ccaacctgga gggtgtacag caccggctct     1920

aacgtgttcc agaccagagc tggatgcctg atcggagctg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgccggcatc tgtgcctctt accagaccca gaccaactct     2040

ccagctgccg cccggtccgt ggcttctcag tccatcatcg cctacaccat gagcctgggc     2100

gccgaaaact ctgtggccta ctccaacaac agcatcgcca tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gcccgtgagc atgaccaaga cctctgtgga ctgcaccatg     2220

tacatctgtg gcgactctac cgaatgctcc aacctgctgc tgcagtacgg ctccttctgt     2280

acccagctga accgcgccct gaccggaatc gctgtggagc aggacaaaaa cacccaggaa     2340

gtgttcgccc aggtgaagca gatctacaaa acccccccca tcaaggactt cggcggcttc     2400

aacttctccc agatcctgcc cgacccctct aagccctcca aaaggagctt catcgaggac     2460

ctgctgttca acaaggtgac cctggccgac gccggcttca tcaaacagta cggcgactgc     2520

ctgggcgaca tcgctgctag agacctgatc tgtgcccaga agttcaacgg actgaccgtg     2580

ctgccaccac tgctgaccga cgaaatgatc gctcagtaca cctccgccct gctggctgga     2640

accatcacca gcggatggac cttcggcgct ggagccgccc tgcagatccc cttcgccatg     2700

cagatggcct acaggttcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaaa tccaggactc cctgtccagc     2820

accgctagcg ccctgggcaa actgcaggac gtggtgaacc agaacgccca ggccctgaac     2880

accctggtga agcagctgtc ttccaacttc ggcgccatca gctctgtgct gaacgacatc     2940

ctgtcccggc tggacaaagt ggaggccgaa gtgcagatcg acaggctgat caccggccgc     3000

ctgcagtctc tgcagaccta cgtgacccag cagctgatca gggccgccga aatcagagcc     3060

tccgccaacc tggccgccac caagatgagc gagtgcgtgc tgggccagtc taagcgcgtg     3120

gacttctgtg gcaaaggcta ccacctgatg agcttcccac agtctgcccc acacggagtg     3180

gtgttcctgc acgtgaccta cgtgcccgcc caggagaaga acttcaccac cgctccagct     3240

atctgccacg acggcaaagc tcacttccca agggaaggcg tgttcgtgag caacggcacc     3300

cactggttcg tgacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtccg gcaactgtga cgtggtcatc ggaatcgtga acaacaccgt gtacgaccca     3420

ctgcagccag agctggactc cttcaaggag gaactggaca agtacttcaa aaaccacacc     3480

agcccagacg tggacctggg cgacatctcc ggcatcaacg ccagcgtggt gaacatccag     3540

aaggagatcg acaggctgaa cgaagtggcc aaaaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aaatccagca tcgcctcctt cttcttcatc     3660

atcggcctga tcatcggcct gttcctggtg ctgagagtgg gcatctacct gtgcatcaag     3720

ctgaaacaca ccaagaaacg gcagatctac accgacatcg agatgaaccg cctgggcaag     3780

tga                                                                   3783


<210>  10
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified Severe Acute Respiratory Syndrome 
       Coronavirus 2 and vesicular stomatitis virus

<400>  10

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ala Ala Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Ser Ser Ile Ala  Ser Phe Phe 
    1205                 1210                 1215             


Phe Ile  Ile Gly Leu Ile Ile  Gly Leu Phe Leu Val  Leu Arg Val 
    1220                 1225                 1230             


Gly Ile  Tyr Leu Cys Ile Lys  Leu Lys His Thr Lys  Lys Arg Gln 
    1235                 1240                 1245             


Ile Tyr  Thr Asp Ile Glu Met  Asn Arg Leu Gly Lys  
    1250                 1255                 1260 


<210>  11
<211>  3783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified Severe Acute Respiratory Syndrome 
       Coronavirus 2 and vesicular stomatitis virus

<400>  11
atgttcgtgt tcctggtgct gctgcccctg gtgtccagcc agtgcgtgaa cctgaccacc       60

agaacccagc tgccaccagc ctacaccaac agcttcaccc ggggcgtgta ctaccccgac      120

aaagtgttcc gctcttccgt gctgcactct acccaggacc tgttcctgcc cttcttctcc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca acggcaccaa gaggttcgac      240

aaccccgtgc tgcccttcaa cgacggcgtg tacttcgcct ctaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccaccctg gacagcaaaa cccagtctct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttctgtaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaaa tcctggatgg agagcgagtt cagggtgtac      480

agctctgcca acaactgtac cttcgagtac gtgagccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa aaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actctaaaca cacccccatc aacctggtgc gcgacctgcc acagggcttc      660

tccgccctgg agccactggt ggacctgccc atcggcatca acatcaccag gttccagacc      720

ctgctggccc tgcaccgctc ctacctgacc ccaggcgact ccagctctgg atggaccgct      780

ggagctgccg cctactacgt gggctacctg cagcccagga ccttcctgct gaaatacaac      840

gaaaacggaa ccatcaccga cgctgtggac tgcgctctgg acccactgtc cgaaaccaag      900

tgtaccctga aaagcttcac cgtggagaag ggcatctacc agaccagcaa cttcagggtg      960

cagcccaccg aatctatcgt gagattcccc aacatcacca acctgtgccc cttcggcgag     1020

gtgttcaacg ccaccagatt cgccagcgtg tacgcctgga acaggaaaag aatctctaac     1080

tgcgtggccg actactccgt gctgtacaac tctgcctcct tcagcacctt caagtgctac     1140

ggcgtgagcc ccaccaaact gaacgacctg tgcttcacca acgtgtacgc cgactctttc     1200

gtgatcaggg gcgacgaggt gagacagatc gctccaggac agaccggcaa gatcgctgac     1260

tacaactaca aactgcccga cgacttcacc ggctgcgtga tcgcctggaa ctccaacaac     1320

ctggacagca aagtgggcgg caactacaac tacctgtacc ggctgttccg caagagcaac     1380

ctgaaaccct tcgagcggga catctctacc gaaatctacc aggctggatc caccccatgc     1440

aacggagtgg agggcttcaa ctgttacttc cccctgcagt cctacggctt ccagccaacc     1500

aacggagtgg gataccagcc atacagggtg gtggtgctgt ccttcgaact gctgcacgct     1560

ccagctaccg tgtgcggacc caagaaaagc accaacctgg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggactgac cggaaccggc gtgctgaccg agagcaacaa gaaattcctg     1680

cccttccagc agttcggaag ggacatcgct gacaccaccg acgccgtgag agacccacag     1740

accctggaaa tcctggacat caccccctgc tctttcggcg gcgtgtccgt gatcacccca     1800

ggaaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg taccgaggtg     1860

ccagtggcta tccacgctga ccagctgacc ccaacctgga gggtgtacag caccggctct     1920

aacgtgttcc agaccagagc tggatgcctg atcggagctg agcacgtgaa caacagctac     1980

gaatgcgaca tccccatcgg cgccggcatc tgtgcctctt accagaccca gaccaactct     2040

ccagctgccg cccggtccgt ggcttctcag tccatcatcg cctacaccat gagcctgggc     2100

gccgaaaact ctgtggccta ctccaacaac agcatcgcca tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gcccgtgagc atgaccaaga cctctgtgga ctgcaccatg     2220

tacatctgtg gcgactctac cgaatgctcc aacctgctgc tgcagtacgg ctccttctgt     2280

acccagctga accgcgccct gaccggaatc gctgtggagc aggacaaaaa cacccaggaa     2340

gtgttcgccc aggtgaagca gatctacaaa acccccccca tcaaggactt cggcggcttc     2400

aacttctccc agatcctgcc cgacccctct aagccctcca aaaggagctt catcgaggac     2460

ctgctgttca acaaggtgac cctggccgac gccggcttca tcaaacagta cggcgactgc     2520

ctgggcgaca tcgctgctag agacctgatc tgtgcccaga agttcaacgg actgaccgtg     2580

ctgccaccac tgctgaccga cgaaatgatc gctcagtaca cctccgccct gctggctgga     2640

accatcacca gcggatggac cttcggcgct ggagccgccc tgcagatccc cttcgccatg     2700

cagatggcct acaggttcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaaa tccaggactc cctgtccagc     2820

accgctagcg ccctgggcaa actgcaggac gtggtgaacc agaacgccca ggccctgaac     2880

accctggtga agcagctgtc ttccaacttc ggcgccatca gctctgtgct gaacgacatc     2940

ctgtccaggc tggacccacc agaggctgaa gtgcagatcg acaggctgat caccggccgc     3000

ctgcagtctc tgcagaccta cgtgacccag cagctgatca gggccgccga aatcagagcc     3060

tccgccaacc tggccgccac caagatgagc gagtgcgtgc tgggccagtc taagcgcgtg     3120

gacttctgtg gcaaaggcta ccacctgatg agcttcccac agtctgcccc acacggagtg     3180

gtgttcctgc acgtgaccta cgtgcccgcc caggagaaga acttcaccac cgctccagct     3240

atctgccacg acggcaaagc tcacttccca agggaaggcg tgttcgtgag caacggcacc     3300

cactggttcg tgacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtccg gcaactgtga cgtggtcatc ggaatcgtga acaacaccgt gtacgaccca     3420

ctgcagccag agctggactc cttcaaggag gaactggaca agtacttcaa aaaccacacc     3480

agcccagacg tggacctggg cgacatctcc ggcatcaacg ccagcgtggt gaacatccag     3540

aaggagatcg acaggctgaa cgaagtggcc aaaaacctga acgaaagcct gatcgacctg     3600

caggagctgg gcaagtacga acagtacatc aaatccagca tcgcctcctt cttcttcatc     3660

atcggcctga tcatcggcct gttcctggtg ctgagagtgg gcatctacct gtgcatcaag     3720

ctgaaacaca ccaagaaacg gcagatctac accgacatcg agatgaaccg cctgggcaag     3780

tga                                                                   3783


<210>  12
<211>  1260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric sequence of modified Severe Acute Respiratory Syndrome 
       Coronavirus 2 and vesicular stomatitis virus

<400>  12

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ala Ala Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Ser Ser Ile Ala  Ser Phe Phe 
    1205                 1210                 1215             


Phe Ile  Ile Gly Leu Ile Ile  Gly Leu Phe Leu Val  Leu Arg Val 
    1220                 1225                 1230             


Gly Ile  Tyr Leu Cys Ile Lys  Leu Lys His Thr Lys  Lys Arg Gln 
    1235                 1240                 1245             


Ile Tyr  Thr Asp Ile Glu Met  Asn Arg Leu Gly Lys  
    1250                 1255                 1260 


<210>  13
<211>  4
<212>  PRT
<213>  Severe Acute Respiratory Syndrome Coronavirus 2

<400>  13

Arg Arg Ala Arg 
1               


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified furin cleavage site IBV

<400>  14

Ala Ala Phe Ala Ala 
1               5   


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  modified furin cleavage site SARS-CoV2

<400>  15

Ala Ala Ala Arg 
1               


<210>  16
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus sequence of 130 BCoV spike protein genes from 2016 - 
       2021


<220>
<221>  CDS
<222>  (1)..(4092)
<223>  Consensus BCoV Spike protein

<400>  16
atg ttt ctg att ctg ctg att agc ctg cca acc gct ttt gct gtc atc         48
Met Phe Leu Ile Leu Leu Ile Ser Leu Pro Thr Ala Phe Ala Val Ile           
1               5                   10                  15                

ggg gac ctg aaa tgc acc acc gtg agt att aac gac gtg gac aca ggc         96
Gly Asp Leu Lys Cys Thr Thr Val Ser Ile Asn Asp Val Asp Thr Gly           
            20                  25                  30                    

gtg cct tcc atc agc acc gac acc gtg gac gtg acc aac ggc ctg ggc        144
Val Pro Ser Ile Ser Thr Asp Thr Val Asp Val Thr Asn Gly Leu Gly           
        35                  40                  45                        

acc tac tac gtg ctg gac cgc gtg tac ctg aac acc acc ctg ctg ctg        192
Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Thr Thr Leu Leu Leu           
    50                  55                  60                            

aac ggc tac tac cct acc tcc ggc agc acc tac aga aac atg gcc ctg        240
Asn Gly Tyr Tyr Pro Thr Ser Gly Ser Thr Tyr Arg Asn Met Ala Leu           
65                  70                  75                  80            

aag ggc acc ctg ctg ctg tcc acc ctg tgg ttc aag ccc cct ttc ctg        288
Lys Gly Thr Leu Leu Leu Ser Thr Leu Trp Phe Lys Pro Pro Phe Leu           
                85                  90                  95                

agc gac ttc acc aac ggc atc ttc gcc aag gtg aag aac acc aaa gtg        336
Ser Asp Phe Thr Asn Gly Ile Phe Ala Lys Val Lys Asn Thr Lys Val           
            100                 105                 110                   

atc aag gac ggc gtg atg tac tcc gag ttc ccc gcc atc acc atc ggc        384
Ile Lys Asp Gly Val Met Tyr Ser Glu Phe Pro Ala Ile Thr Ile Gly           
        115                 120                 125                       

agc acc ttc gtg aac acc tcc tac agc gtg gtg gtg cag cct cac acc        432
Ser Thr Phe Val Asn Thr Ser Tyr Ser Val Val Val Gln Pro His Thr           
    130                 135                 140                           

acc atc ctg ggc aac aag ctg cag ggc ttc ctg gag atc tcc gtg tgc        480
Thr Ile Leu Gly Asn Lys Leu Gln Gly Phe Leu Glu Ile Ser Val Cys           
145                 150                 155                 160           

cag tac acc atg tgc gag tac ccc aac acc atc tgc aac cct aac ctg        528
Gln Tyr Thr Met Cys Glu Tyr Pro Asn Thr Ile Cys Asn Pro Asn Leu           
                165                 170                 175               

ggc aac cag agg gtg gag ctg tgg cac tgg gac aca ggc gtg gtg agc        576
Gly Asn Gln Arg Val Glu Leu Trp His Trp Asp Thr Gly Val Val Ser           
            180                 185                 190                   

tgc ctg tac aag agg aac ttc acc tac gac gtg aac gcc gac tac ctg        624
Cys Leu Tyr Lys Arg Asn Phe Thr Tyr Asp Val Asn Ala Asp Tyr Leu           
        195                 200                 205                       

tac ttc cac ttc tac cag gag ggc ggc acc ttc tac gcc tac ttc acc        672
Tyr Phe His Phe Tyr Gln Glu Gly Gly Thr Phe Tyr Ala Tyr Phe Thr           
    210                 215                 220                           

gac aca ggc gtg gtg acc aag ttc ctg ttc aac gtg tac ctg ggc acc        720
Asp Thr Gly Val Val Thr Lys Phe Leu Phe Asn Val Tyr Leu Gly Thr           
225                 230                 235                 240           

gtg ctg tcc cac tac tac gtg atg ccc ctg acc tgc aac agc gcc ctg        768
Val Leu Ser His Tyr Tyr Val Met Pro Leu Thr Cys Asn Ser Ala Leu           
                245                 250                 255               

acc ctg gag tac tgg gtg acc cct ctg acc agc aag cag tac ctg ctg        816
Thr Leu Glu Tyr Trp Val Thr Pro Leu Thr Ser Lys Gln Tyr Leu Leu           
            260                 265                 270                   

gcc ttc aac cag gac ggc gtg atc ttc aac gcc gtg gac tgc aag tcc        864
Ala Phe Asn Gln Asp Gly Val Ile Phe Asn Ala Val Asp Cys Lys Ser           
        275                 280                 285                       

gac ttc atg agc gag atc aag tgc aag acc ctg tcc atc gct cct agc        912
Asp Phe Met Ser Glu Ile Lys Cys Lys Thr Leu Ser Ile Ala Pro Ser           
    290                 295                 300                           

acc ggg gtg tac gag ctg aac ggc tac acc gtg cag cct atc gcc gac        960
Thr Gly Val Tyr Glu Leu Asn Gly Tyr Thr Val Gln Pro Ile Ala Asp           
305                 310                 315                 320           

gtg tac cgg agg atc ccc aac ctg cct gac tgc aac atc gag gcc tgg       1008
Val Tyr Arg Arg Ile Pro Asn Leu Pro Asp Cys Asn Ile Glu Ala Trp           
                325                 330                 335               

ctg aac gac aag tcc gtg ccc agc cct ctg aac tgg gag cgg aag acc       1056
Leu Asn Asp Lys Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr           
            340                 345                 350                   

ttc agc aac tgc aac ttc aac atg agc tcc ctg atg tcc ttc atc cag       1104
Phe Ser Asn Cys Asn Phe Asn Met Ser Ser Leu Met Ser Phe Ile Gln           
        355                 360                 365                       

gcc gac agc ttc acc tgc aac aac atc gac gcc gcc aag atc tac ggc       1152
Ala Asp Ser Phe Thr Cys Asn Asn Ile Asp Ala Ala Lys Ile Tyr Gly           
    370                 375                 380                           

atg tgc ttc agc tcc atc acc atc gac aag ttc gcc atc ccc aac ggc       1200
Met Cys Phe Ser Ser Ile Thr Ile Asp Lys Phe Ala Ile Pro Asn Gly           
385                 390                 395                 400           

cgg aag gtg gac ctg cag ctg ggc aac ctg ggc tac ctg cag tcc ttc       1248
Arg Lys Val Asp Leu Gln Leu Gly Asn Leu Gly Tyr Leu Gln Ser Phe           
                405                 410                 415               

aac tac agg atc gac acc acc gcc acc agc tgc cag ctg tac tac aac       1296
Asn Tyr Arg Ile Asp Thr Thr Ala Thr Ser Cys Gln Leu Tyr Tyr Asn           
            420                 425                 430                   

ctg cct gct gct aac gtg tcc gtg agc agg ttc aac cct agc acc tgg       1344
Leu Pro Ala Ala Asn Val Ser Val Ser Arg Phe Asn Pro Ser Thr Trp           
        435                 440                 445                       

aac cgc aga ttc ggc ttc acc gag cag tcc gtg ttc aag ccc cag cct       1392
Asn Arg Arg Phe Gly Phe Thr Glu Gln Ser Val Phe Lys Pro Gln Pro           
    450                 455                 460                           

gcc ggc gtg ttc acc gac cac gac gtg gtg tac gcc cag cac tgc ttc       1440
Ala Gly Val Phe Thr Asp His Asp Val Val Tyr Ala Gln His Cys Phe           
465                 470                 475                 480           

aag gcc ccc acc aac ttc tgc cct tgc aag ctg gac ggc agc ctg tgc       1488
Lys Ala Pro Thr Asn Phe Cys Pro Cys Lys Leu Asp Gly Ser Leu Cys           
                485                 490                 495               

gtg gga tcc gga agc ggc atc gac gcc ggc tac aag aac acc ggc atc       1536
Val Gly Ser Gly Ser Gly Ile Asp Ala Gly Tyr Lys Asn Thr Gly Ile           
            500                 505                 510                   

gga acc tgc cct gct gga acc aac tac ctg acc tgc cac aac gct gct       1584
Gly Thr Cys Pro Ala Gly Thr Asn Tyr Leu Thr Cys His Asn Ala Ala           
        515                 520                 525                       

cag tgc gac tgc ctg tgc acc ccc gac cct atc acc tcc aag gcc acc       1632
Gln Cys Asp Cys Leu Cys Thr Pro Asp Pro Ile Thr Ser Lys Ala Thr           
    530                 535                 540                           

ggc ccc tac aag tgc cct cag acc aag tac ctg gtg gga atc gga gag       1680
Gly Pro Tyr Lys Cys Pro Gln Thr Lys Tyr Leu Val Gly Ile Gly Glu           
545                 550                 555                 560           

cac tgc tcc ggc ctg gcc atc aag agc gac cac tgc gga gga aac cct       1728
His Cys Ser Gly Leu Ala Ile Lys Ser Asp His Cys Gly Gly Asn Pro           
                565                 570                 575               

tgc agc tgc cag cct cag gcc ttc ctg gga tgg tcc gtg gac agc tgc       1776
Cys Ser Cys Gln Pro Gln Ala Phe Leu Gly Trp Ser Val Asp Ser Cys           
            580                 585                 590                   

ctg cag ggc gac cgc tgc aac atc ttc gcc aac ttc atc ctg cac gac       1824
Leu Gln Gly Asp Arg Cys Asn Ile Phe Ala Asn Phe Ile Leu His Asp           
        595                 600                 605                       

gtg aac tcc ggc acc acc tgc agc acc gac ctg cag aag agc aac acc       1872
Val Asn Ser Gly Thr Thr Cys Ser Thr Asp Leu Gln Lys Ser Asn Thr           
    610                 615                 620                           

gac atc atc ctg ggc gtg tgc gtg aac tac gac ctg tac ggc atc acc       1920
Asp Ile Ile Leu Gly Val Cys Val Asn Tyr Asp Leu Tyr Gly Ile Thr           
625                 630                 635                 640           

ggc cag ggc atc ttc gtg gag gtg aac gcc acc tac tac aac tcc tgg       1968
Gly Gln Gly Ile Phe Val Glu Val Asn Ala Thr Tyr Tyr Asn Ser Trp           
                645                 650                 655               

cag aac ctg ctg tac gac agc aac ggc aac ctg tac ggc ttc cgc gac       2016
Gln Asn Leu Leu Tyr Asp Ser Asn Gly Asn Leu Tyr Gly Phe Arg Asp           
            660                 665                 670                   

tac ctg acc aac cgg acc ttc atg atc agg tcc tgc tac agc ggc aga       2064
Tyr Leu Thr Asn Arg Thr Phe Met Ile Arg Ser Cys Tyr Ser Gly Arg           
        675                 680                 685                       

gtg agc gcc gct ttc cac gcc aac agc tcc gag ccc gcc ctg ctg ttc       2112
Val Ser Ala Ala Phe His Ala Asn Ser Ser Glu Pro Ala Leu Leu Phe           
    690                 695                 700                           

cgg aac atc aag tgc aac tac gtg ttc aac aac acc ctg tcc agg cag       2160
Arg Asn Ile Lys Cys Asn Tyr Val Phe Asn Asn Thr Leu Ser Arg Gln           
705                 710                 715                 720           

ctg cag cct atc aac tac ttc gac agc tac ctg ggc tgc gtg gtg aac       2208
Leu Gln Pro Ile Asn Tyr Phe Asp Ser Tyr Leu Gly Cys Val Val Asn           
                725                 730                 735               

gcc gac aac tcc acc agc tcc gtg gtg cag acc tgc gac ctg acc gtg       2256
Ala Asp Asn Ser Thr Ser Ser Val Val Gln Thr Cys Asp Leu Thr Val           
            740                 745                 750                   

gga tcc gga tac tgc gtg gac tac agc acc aag cgg agg tcc cgc aga       2304
Gly Ser Gly Tyr Cys Val Asp Tyr Ser Thr Lys Arg Arg Ser Arg Arg           
        755                 760                 765                       

agc atc acc acc ggc tac aga ttc acc aac ttc gag ccc ttc acc gtg       2352
Ser Ile Thr Thr Gly Tyr Arg Phe Thr Asn Phe Glu Pro Phe Thr Val           
    770                 775                 780                           

aac tcc gtg aac gac agc ctg gag ccc gtg gga ggc ctg tac gag atc       2400
Asn Ser Val Asn Asp Ser Leu Glu Pro Val Gly Gly Leu Tyr Glu Ile           
785                 790                 795                 800           

cag atc cct agc gag ttc acc atc ggc aac atg gag gag ttc atc cag       2448
Gln Ile Pro Ser Glu Phe Thr Ile Gly Asn Met Glu Glu Phe Ile Gln           
                805                 810                 815               

acc agc tcc cct aag gtg acc atc gac tgc tcc gcc ttc gtg tgc ggc       2496
Thr Ser Ser Pro Lys Val Thr Ile Asp Cys Ser Ala Phe Val Cys Gly           
            820                 825                 830                   

gac tac gct gct tgc aag tcc cag ctg gtg gag tac ggc agc ttc tgc       2544
Asp Tyr Ala Ala Cys Lys Ser Gln Leu Val Glu Tyr Gly Ser Phe Cys           
        835                 840                 845                       

gac aac atc aac gcc atc ctg acc gag gtg aac gag ctg ctg gac acc       2592
Asp Asn Ile Asn Ala Ile Leu Thr Glu Val Asn Glu Leu Leu Asp Thr           
    850                 855                 860                           

acc cag ctg cag gtg gcc aac tcc ctg atg aac ggc gtg acc ctg agc       2640
Thr Gln Leu Gln Val Ala Asn Ser Leu Met Asn Gly Val Thr Leu Ser           
865                 870                 875                 880           

acc aag ctg aag gac ggc gtg aac ttc aac gtg gac gat atc aac ttc       2688
Thr Lys Leu Lys Asp Gly Val Asn Phe Asn Val Asp Asp Ile Asn Phe           
                885                 890                 895               

tcc ccc gtg ctg gga tgc ctg gga agc gac tgc aac aag gtg agc tcc       2736
Ser Pro Val Leu Gly Cys Leu Gly Ser Asp Cys Asn Lys Val Ser Ser           
            900                 905                 910                   

cgg tcc gcc atc gag gac ctg ctg ttc tcc aag gtg aag ctg agc gac       2784
Arg Ser Ala Ile Glu Asp Leu Leu Phe Ser Lys Val Lys Leu Ser Asp           
        915                 920                 925                       

gtg ggc ttc gtg gag gcc tac aac aac tgc aca ggc gga gct gag atc       2832
Val Gly Phe Val Glu Ala Tyr Asn Asn Cys Thr Gly Gly Ala Glu Ile           
    930                 935                 940                           

agg gac ctg atc tgc gtg cag agc tac aac ggc atc aag gtg ctg ccc       2880
Arg Asp Leu Ile Cys Val Gln Ser Tyr Asn Gly Ile Lys Val Leu Pro           
945                 950                 955                 960           

cct ctg ctg tcc gag aac cag atc agc ggc tac acc ctg gct gcc acc       2928
Pro Leu Leu Ser Glu Asn Gln Ile Ser Gly Tyr Thr Leu Ala Ala Thr           
                965                 970                 975               

tcc gcc agc ctg ttc cct cct tgg agc gcc gcc gcc ggg gtg ccc ttc       2976
Ser Ala Ser Leu Phe Pro Pro Trp Ser Ala Ala Ala Gly Val Pro Phe           
            980                 985                 990                   

tac ctg aac gtg cag tac cgc atc  aac ggc atc ggc gtg  acc atg gac     3024
Tyr Leu Asn Val Gln Tyr Arg Ile  Asn Gly Ile Gly Val  Thr Met Asp         
        995                 1000                 1005                     

gtg ctg  agc cag aac cag aag  ctg atc gcc aac gcc  ttc aac aac        3069
Val Leu  Ser Gln Asn Gln Lys  Leu Ile Ala Asn Ala  Phe Asn Asn            
    1010                 1015                 1020                        

gct ctg  gga gct atc cag gag  gga ttc gac gct acc  aac agc gcc        3114
Ala Leu  Gly Ala Ile Gln Glu  Gly Phe Asp Ala Thr  Asn Ser Ala            
    1025                 1030                 1035                        

ctg gtg  aag atc cag gct gtg  gtg aac gct aac gct  gag gcc ctg        3159
Leu Val  Lys Ile Gln Ala Val  Val Asn Ala Asn Ala  Glu Ala Leu            
    1040                 1045                 1050                        

aac aac  ctg ctg cag cag ctg  agc aac cgc ttc ggc  gcc atc agc        3204
Asn Asn  Leu Leu Gln Gln Leu  Ser Asn Arg Phe Gly  Ala Ile Ser            
    1055                 1060                 1065                        

tcc agc  ctg cag gag atc ctg  tcc aga ctg gac gcc  ctg gag gct        3249
Ser Ser  Leu Gln Glu Ile Leu  Ser Arg Leu Asp Ala  Leu Glu Ala            
    1070                 1075                 1080                        

cag gct  cag atc gac cgc ctg  atc aac ggc aga ctg  acc gcc ctg        3294
Gln Ala  Gln Ile Asp Arg Leu  Ile Asn Gly Arg Leu  Thr Ala Leu            
    1085                 1090                 1095                        

aac gcc  tac gtg agc cag cag  ctg tcc gac agc acc  ctg gtg aag        3339
Asn Ala  Tyr Val Ser Gln Gln  Leu Ser Asp Ser Thr  Leu Val Lys            
    1100                 1105                 1110                        

ttc tcc  gcc gcc cag gcc atg  gag aag gtg aac gag  tgc gtg aag        3384
Phe Ser  Ala Ala Gln Ala Met  Glu Lys Val Asn Glu  Cys Val Lys            
    1115                 1120                 1125                        

agc cag  tcc agc cgc atc aac  ttc tgc ggc aac ggc  aac cac atc        3429
Ser Gln  Ser Ser Arg Ile Asn  Phe Cys Gly Asn Gly  Asn His Ile            
    1130                 1135                 1140                        

atc tcc  ctg gtg cag aac gcc  ccc tac ggc ctg tac  ttc atc cac        3474
Ile Ser  Leu Val Gln Asn Ala  Pro Tyr Gly Leu Tyr  Phe Ile His            
    1145                 1150                 1155                        

ttc agc  tac gtg ccc acc aag  tac gtg acc gcc aag  gtg tcc cct        3519
Phe Ser  Tyr Val Pro Thr Lys  Tyr Val Thr Ala Lys  Val Ser Pro            
    1160                 1165                 1170                        

ggc ctg  tgc atc gcc ggg gac  cgg ggc atc gcc cct  aag agc ggc        3564
Gly Leu  Cys Ile Ala Gly Asp  Arg Gly Ile Ala Pro  Lys Ser Gly            
    1175                 1180                 1185                        

tac ttc  gtg aac gtg aac aac  acc tgg atg ttc acc  ggc tcc ggc        3609
Tyr Phe  Val Asn Val Asn Asn  Thr Trp Met Phe Thr  Gly Ser Gly            
    1190                 1195                 1200                        

tac tac  tac ccc gag cct atc  acc ggc aac aac gtg  gtg gtc atg        3654
Tyr Tyr  Tyr Pro Glu Pro Ile  Thr Gly Asn Asn Val  Val Val Met            
    1205                 1210                 1215                        

agc acc  tgc gcc gtg aac tac  acc aag gcc ccc gac  gtg atg ctg        3699
Ser Thr  Cys Ala Val Asn Tyr  Thr Lys Ala Pro Asp  Val Met Leu            
    1220                 1225                 1230                        

aac atc  tcc acc ccc aac ctg  cct gac ttc aag gag  gag ctg gac        3744
Asn Ile  Ser Thr Pro Asn Leu  Pro Asp Phe Lys Glu  Glu Leu Asp            
    1235                 1240                 1245                        

cag tgg  ttc aag aac cag acc  tcc gtg gcc cct gac  ctg agc ctg        3789
Gln Trp  Phe Lys Asn Gln Thr  Ser Val Ala Pro Asp  Leu Ser Leu            
    1250                 1255                 1260                        

gac tac  atc aac gtg acc ttc  ctg gac ctg cag gac  gag atg aac        3834
Asp Tyr  Ile Asn Val Thr Phe  Leu Asp Leu Gln Asp  Glu Met Asn            
    1265                 1270                 1275                        

agg ctg  cag gag gcc atc aag  gtg ctg aac cag tcc  tac atc aac        3879
Arg Leu  Gln Glu Ala Ile Lys  Val Leu Asn Gln Ser  Tyr Ile Asn            
    1280                 1285                 1290                        

ctg aag  gac atc ggc acc tac  gag tac tac gtg aag  tgg ccc tgg        3924
Leu Lys  Asp Ile Gly Thr Tyr  Glu Tyr Tyr Val Lys  Trp Pro Trp            
    1295                 1300                 1305                        

tac gtg  tgg ctg ctg atc ggc  ttt gcc ggc gtg gcc  atg ctg gtg        3969
Tyr Val  Trp Leu Leu Ile Gly  Phe Ala Gly Val Ala  Met Leu Val            
    1310                 1315                 1320                        

ctg ctg  ttc ttc atc tgc tgc  tgc acc ggc tgc ggc  acc agc tgc        4014
Leu Leu  Phe Phe Ile Cys Cys  Cys Thr Gly Cys Gly  Thr Ser Cys            
    1325                 1330                 1335                        

ttc aag  aag tgc ggc ggc tgc  tgc gac gat tac act  gga cat cag        4059
Phe Lys  Lys Cys Gly Gly Cys  Cys Asp Asp Tyr Thr  Gly His Gln            
    1340                 1345                 1350                        

gaa ctg  gtc atc aaa aca tca  cac gag gac taa                         4092
Glu Leu  Val Ile Lys Thr Ser  His Glu Asp                                 
    1355                 1360                                             


<210>  17
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Met Phe Leu Ile Leu Leu Ile Ser Leu Pro Thr Ala Phe Ala Val Ile 
1               5                   10                  15      


Gly Asp Leu Lys Cys Thr Thr Val Ser Ile Asn Asp Val Asp Thr Gly 
            20                  25                  30          


Val Pro Ser Ile Ser Thr Asp Thr Val Asp Val Thr Asn Gly Leu Gly 
        35                  40                  45              


Thr Tyr Tyr Val Leu Asp Arg Val Tyr Leu Asn Thr Thr Leu Leu Leu 
    50                  55                  60                  


Asn Gly Tyr Tyr Pro Thr Ser Gly Ser Thr Tyr Arg Asn Met Ala Leu 
65                  70                  75                  80  


Lys Gly Thr Leu Leu Leu Ser Thr Leu Trp Phe Lys Pro Pro Phe Leu 
                85                  90                  95      


Ser Asp Phe Thr Asn Gly Ile Phe Ala Lys Val Lys Asn Thr Lys Val 
            100                 105                 110         


Ile Lys Asp Gly Val Met Tyr Ser Glu Phe Pro Ala Ile Thr Ile Gly 
        115                 120                 125             


Ser Thr Phe Val Asn Thr Ser Tyr Ser Val Val Val Gln Pro His Thr 
    130                 135                 140                 


Thr Ile Leu Gly Asn Lys Leu Gln Gly Phe Leu Glu Ile Ser Val Cys 
145                 150                 155                 160 


Gln Tyr Thr Met Cys Glu Tyr Pro Asn Thr Ile Cys Asn Pro Asn Leu 
                165                 170                 175     


Gly Asn Gln Arg Val Glu Leu Trp His Trp Asp Thr Gly Val Val Ser 
            180                 185                 190         


Cys Leu Tyr Lys Arg Asn Phe Thr Tyr Asp Val Asn Ala Asp Tyr Leu 
        195                 200                 205             


Tyr Phe His Phe Tyr Gln Glu Gly Gly Thr Phe Tyr Ala Tyr Phe Thr 
    210                 215                 220                 


Asp Thr Gly Val Val Thr Lys Phe Leu Phe Asn Val Tyr Leu Gly Thr 
225                 230                 235                 240 


Val Leu Ser His Tyr Tyr Val Met Pro Leu Thr Cys Asn Ser Ala Leu 
                245                 250                 255     


Thr Leu Glu Tyr Trp Val Thr Pro Leu Thr Ser Lys Gln Tyr Leu Leu 
            260                 265                 270         


Ala Phe Asn Gln Asp Gly Val Ile Phe Asn Ala Val Asp Cys Lys Ser 
        275                 280                 285             


Asp Phe Met Ser Glu Ile Lys Cys Lys Thr Leu Ser Ile Ala Pro Ser 
    290                 295                 300                 


Thr Gly Val Tyr Glu Leu Asn Gly Tyr Thr Val Gln Pro Ile Ala Asp 
305                 310                 315                 320 


Val Tyr Arg Arg Ile Pro Asn Leu Pro Asp Cys Asn Ile Glu Ala Trp 
                325                 330                 335     


Leu Asn Asp Lys Ser Val Pro Ser Pro Leu Asn Trp Glu Arg Lys Thr 
            340                 345                 350         


Phe Ser Asn Cys Asn Phe Asn Met Ser Ser Leu Met Ser Phe Ile Gln 
        355                 360                 365             


Ala Asp Ser Phe Thr Cys Asn Asn Ile Asp Ala Ala Lys Ile Tyr Gly 
    370                 375                 380                 


Met Cys Phe Ser Ser Ile Thr Ile Asp Lys Phe Ala Ile Pro Asn Gly 
385                 390                 395                 400 


Arg Lys Val Asp Leu Gln Leu Gly Asn Leu Gly Tyr Leu Gln Ser Phe 
                405                 410                 415     


Asn Tyr Arg Ile Asp Thr Thr Ala Thr Ser Cys Gln Leu Tyr Tyr Asn 
            420                 425                 430         


Leu Pro Ala Ala Asn Val Ser Val Ser Arg Phe Asn Pro Ser Thr Trp 
        435                 440                 445             


Asn Arg Arg Phe Gly Phe Thr Glu Gln Ser Val Phe Lys Pro Gln Pro 
    450                 455                 460                 


Ala Gly Val Phe Thr Asp His Asp Val Val Tyr Ala Gln His Cys Phe 
465                 470                 475                 480 


Lys Ala Pro Thr Asn Phe Cys Pro Cys Lys Leu Asp Gly Ser Leu Cys 
                485                 490                 495     


Val Gly Ser Gly Ser Gly Ile Asp Ala Gly Tyr Lys Asn Thr Gly Ile 
            500                 505                 510         


Gly Thr Cys Pro Ala Gly Thr Asn Tyr Leu Thr Cys His Asn Ala Ala 
        515                 520                 525             


Gln Cys Asp Cys Leu Cys Thr Pro Asp Pro Ile Thr Ser Lys Ala Thr 
    530                 535                 540                 


Gly Pro Tyr Lys Cys Pro Gln Thr Lys Tyr Leu Val Gly Ile Gly Glu 
545                 550                 555                 560 


His Cys Ser Gly Leu Ala Ile Lys Ser Asp His Cys Gly Gly Asn Pro 
                565                 570                 575     


Cys Ser Cys Gln Pro Gln Ala Phe Leu Gly Trp Ser Val Asp Ser Cys 
            580                 585                 590         


Leu Gln Gly Asp Arg Cys Asn Ile Phe Ala Asn Phe Ile Leu His Asp 
        595                 600                 605             


Val Asn Ser Gly Thr Thr Cys Ser Thr Asp Leu Gln Lys Ser Asn Thr 
    610                 615                 620                 


Asp Ile Ile Leu Gly Val Cys Val Asn Tyr Asp Leu Tyr Gly Ile Thr 
625                 630                 635                 640 


Gly Gln Gly Ile Phe Val Glu Val Asn Ala Thr Tyr Tyr Asn Ser Trp 
                645                 650                 655     


Gln Asn Leu Leu Tyr Asp Ser Asn Gly Asn Leu Tyr Gly Phe Arg Asp 
            660                 665                 670         


Tyr Leu Thr Asn Arg Thr Phe Met Ile Arg Ser Cys Tyr Ser Gly Arg 
        675                 680                 685             


Val Ser Ala Ala Phe His Ala Asn Ser Ser Glu Pro Ala Leu Leu Phe 
    690                 695                 700                 


Arg Asn Ile Lys Cys Asn Tyr Val Phe Asn Asn Thr Leu Ser Arg Gln 
705                 710                 715                 720 


Leu Gln Pro Ile Asn Tyr Phe Asp Ser Tyr Leu Gly Cys Val Val Asn 
                725                 730                 735     


Ala Asp Asn Ser Thr Ser Ser Val Val Gln Thr Cys Asp Leu Thr Val 
            740                 745                 750         


Gly Ser Gly Tyr Cys Val Asp Tyr Ser Thr Lys Arg Arg Ser Arg Arg 
        755                 760                 765             


Ser Ile Thr Thr Gly Tyr Arg Phe Thr Asn Phe Glu Pro Phe Thr Val 
    770                 775                 780                 


Asn Ser Val Asn Asp Ser Leu Glu Pro Val Gly Gly Leu Tyr Glu Ile 
785                 790                 795                 800 


Gln Ile Pro Ser Glu Phe Thr Ile Gly Asn Met Glu Glu Phe Ile Gln 
                805                 810                 815     


Thr Ser Ser Pro Lys Val Thr Ile Asp Cys Ser Ala Phe Val Cys Gly 
            820                 825                 830         


Asp Tyr Ala Ala Cys Lys Ser Gln Leu Val Glu Tyr Gly Ser Phe Cys 
        835                 840                 845             


Asp Asn Ile Asn Ala Ile Leu Thr Glu Val Asn Glu Leu Leu Asp Thr 
    850                 855                 860                 


Thr Gln Leu Gln Val Ala Asn Ser Leu Met Asn Gly Val Thr Leu Ser 
865                 870                 875                 880 


Thr Lys Leu Lys Asp Gly Val Asn Phe Asn Val Asp Asp Ile Asn Phe 
                885                 890                 895     


Ser Pro Val Leu Gly Cys Leu Gly Ser Asp Cys Asn Lys Val Ser Ser 
            900                 905                 910         


Arg Ser Ala Ile Glu Asp Leu Leu Phe Ser Lys Val Lys Leu Ser Asp 
        915                 920                 925             


Val Gly Phe Val Glu Ala Tyr Asn Asn Cys Thr Gly Gly Ala Glu Ile 
    930                 935                 940                 


Arg Asp Leu Ile Cys Val Gln Ser Tyr Asn Gly Ile Lys Val Leu Pro 
945                 950                 955                 960 


Pro Leu Leu Ser Glu Asn Gln Ile Ser Gly Tyr Thr Leu Ala Ala Thr 
                965                 970                 975     


Ser Ala Ser Leu Phe Pro Pro Trp Ser Ala Ala Ala Gly Val Pro Phe 
            980                 985                 990         


Tyr Leu Asn Val Gln Tyr Arg Ile  Asn Gly Ile Gly Val  Thr Met Asp 
        995                 1000                 1005             


Val Leu  Ser Gln Asn Gln Lys  Leu Ile Ala Asn Ala  Phe Asn Asn 
    1010                 1015                 1020             


Ala Leu  Gly Ala Ile Gln Glu  Gly Phe Asp Ala Thr  Asn Ser Ala 
    1025                 1030                 1035             


Leu Val  Lys Ile Gln Ala Val  Val Asn Ala Asn Ala  Glu Ala Leu 
    1040                 1045                 1050             


Asn Asn  Leu Leu Gln Gln Leu  Ser Asn Arg Phe Gly  Ala Ile Ser 
    1055                 1060                 1065             


Ser Ser  Leu Gln Glu Ile Leu  Ser Arg Leu Asp Ala  Leu Glu Ala 
    1070                 1075                 1080             


Gln Ala  Gln Ile Asp Arg Leu  Ile Asn Gly Arg Leu  Thr Ala Leu 
    1085                 1090                 1095             


Asn Ala  Tyr Val Ser Gln Gln  Leu Ser Asp Ser Thr  Leu Val Lys 
    1100                 1105                 1110             


Phe Ser  Ala Ala Gln Ala Met  Glu Lys Val Asn Glu  Cys Val Lys 
    1115                 1120                 1125             


Ser Gln  Ser Ser Arg Ile Asn  Phe Cys Gly Asn Gly  Asn His Ile 
    1130                 1135                 1140             


Ile Ser  Leu Val Gln Asn Ala  Pro Tyr Gly Leu Tyr  Phe Ile His 
    1145                 1150                 1155             


Phe Ser  Tyr Val Pro Thr Lys  Tyr Val Thr Ala Lys  Val Ser Pro 
    1160                 1165                 1170             


Gly Leu  Cys Ile Ala Gly Asp  Arg Gly Ile Ala Pro  Lys Ser Gly 
    1175                 1180                 1185             


Tyr Phe  Val Asn Val Asn Asn  Thr Trp Met Phe Thr  Gly Ser Gly 
    1190                 1195                 1200             


Tyr Tyr  Tyr Pro Glu Pro Ile  Thr Gly Asn Asn Val  Val Val Met 
    1205                 1210                 1215             


Ser Thr  Cys Ala Val Asn Tyr  Thr Lys Ala Pro Asp  Val Met Leu 
    1220                 1225                 1230             


Asn Ile  Ser Thr Pro Asn Leu  Pro Asp Phe Lys Glu  Glu Leu Asp 
    1235                 1240                 1245             


Gln Trp  Phe Lys Asn Gln Thr  Ser Val Ala Pro Asp  Leu Ser Leu 
    1250                 1255                 1260             


Asp Tyr  Ile Asn Val Thr Phe  Leu Asp Leu Gln Asp  Glu Met Asn 
    1265                 1270                 1275             


Arg Leu  Gln Glu Ala Ile Lys  Val Leu Asn Gln Ser  Tyr Ile Asn 
    1280                 1285                 1290             


Leu Lys  Asp Ile Gly Thr Tyr  Glu Tyr Tyr Val Lys  Trp Pro Trp 
    1295                 1300                 1305             


Tyr Val  Trp Leu Leu Ile Gly  Phe Ala Gly Val Ala  Met Leu Val 
    1310                 1315                 1320             


Leu Leu  Phe Phe Ile Cys Cys  Cys Thr Gly Cys Gly  Thr Ser Cys 
    1325                 1330                 1335             


Phe Lys  Lys Cys Gly Gly Cys  Cys Asp Asp Tyr Thr  Gly His Gln 
    1340                 1345                 1350             


Glu Leu  Val Ile Lys Thr Ser  His Glu Asp 
    1355                 1360             


<210>  18
<211>  3390
<212>  DNA
<213>  porcine enteric alphacoronavirus


<220>
<221>  CDS
<222>  (1)..(3390)
<223>  SADS-CoV Spike protein

<400>  18
atg aag ctg ttt acc gtg ttt acc ctg ctc gcc tca atc aga gtg ctc         48
Met Lys Leu Phe Thr Val Phe Thr Leu Leu Ala Ser Ile Arg Val Leu           
1               5                   10                  15                

tat ggc tgt gaa tcc gtg gac ttt aac ctg ttt aat acc atc ttc tcc         96
Tyr Gly Cys Glu Ser Val Asp Phe Asn Leu Phe Asn Thr Ile Phe Ser           
            20                  25                  30                    

acc cac agg ggc ctg tcc aac acc acc agc gtg atc acc ggc gcc tac        144
Thr His Arg Gly Leu Ser Asn Thr Thr Ser Val Ile Thr Gly Ala Tyr           
        35                  40                  45                        

ccc agc acc aac aag tcc gac tgg agc tgc aac acc agg acc gga cac        192
Pro Ser Thr Asn Lys Ser Asp Trp Ser Cys Asn Thr Arg Thr Gly His           
    50                  55                  60                            

ctg tcc gga agc gga ttc gga atc ggc ctg tac gtg cag acc ccc aga        240
Leu Ser Gly Ser Gly Phe Gly Ile Gly Leu Tyr Val Gln Thr Pro Arg           
65                  70                  75                  80            

gag cag tac cag tac gac gga tcc gga gct gga gga tac acc atc gcc        288
Glu Gln Tyr Gln Tyr Asp Gly Ser Gly Ala Gly Gly Tyr Thr Ile Ala           
                85                  90                  95                

gtg agc cca atc cac gtg acc aac ctg acc tgg gag ctg tgg atc cac        336
Val Ser Pro Ile His Val Thr Asn Leu Thr Trp Glu Leu Trp Ile His           
            100                 105                 110                   

aga aag tgg ggc gtg aac tcc gtg gtg acc gtg agg ctg tgc aga tgg        384
Arg Lys Trp Gly Val Asn Ser Val Val Thr Val Arg Leu Cys Arg Trp           
        115                 120                 125                       

tgg cag ttc atg agc ttc aac tcc acc agc cac gct gct gac gct gga        432
Trp Gln Phe Met Ser Phe Asn Ser Thr Ser His Ala Ala Asp Ala Gly           
    130                 135                 140                           

ccc acc aac gcc ttc gag tgc ctg atc aac ggc agc tac cca acc cac        480
Pro Thr Asn Ala Phe Glu Cys Leu Ile Asn Gly Ser Tyr Pro Thr His           
145                 150                 155                 160           

cgc aac acc ggc tac atg ttc ggc gtg acc tgg tac aac gac ctg gtg        528
Arg Asn Thr Gly Tyr Met Phe Gly Val Thr Trp Tyr Asn Asp Leu Val           
                165                 170                 175               

cgg atc gtg ttc ccc cca acc gtg ctg gag atg cag ctg gac ggc ctg        576
Arg Ile Val Phe Pro Pro Thr Val Leu Glu Met Gln Leu Asp Gly Leu           
            180                 185                 190                   

cag tgg gag agg gtg cag ttc aac tcc cca gtg aac gct gga cac gct        624
Gln Trp Glu Arg Val Gln Phe Asn Ser Pro Val Asn Ala Gly His Ala           
        195                 200                 205                       

acc cgg ttc aac gtg gtg aag gac atc tcc acc gtg ctg gtg gag aca        672
Thr Arg Phe Asn Val Val Lys Asp Ile Ser Thr Val Leu Val Glu Thr           
    210                 215                 220                           

aac tcc ggc ggc agc gtg ttc agg tac agc tac tgc gcc gac ggc ttc        720
Asn Ser Gly Gly Ser Val Phe Arg Tyr Ser Tyr Cys Ala Asp Gly Phe           
225                 230                 235                 240           

gtg aac ggc ctg cag tgc aag ctg aga ctg ttc gac atc ccc cca ggc        768
Val Asn Gly Leu Gln Cys Lys Leu Arg Leu Phe Asp Ile Pro Pro Gly           
                245                 250                 255               

gtg tac tcc aac agc gag gtg gag tac cca acc gcc ctg tac acc gtg        816
Val Tyr Ser Asn Ser Glu Val Glu Tyr Pro Thr Ala Leu Tyr Thr Val           
            260                 265                 270                   

gtg cac aac atg tcc gcc tgc ccc gag agg cca gac agc tac tgc ggc        864
Val His Asn Met Ser Ala Cys Pro Glu Arg Pro Asp Ser Tyr Cys Gly           
        275                 280                 285                       

tcc aac agc tgc ccc ttc aag aga gcc gtg ttc agc aac tgc atc gtg        912
Ser Asn Ser Cys Pro Phe Lys Arg Ala Val Phe Ser Asn Cys Ile Val           
    290                 295                 300                           

aac tac acc acc tgg gtg aac cca gac cag cgc gac ttc cag cac ctg        960
Asn Tyr Thr Thr Trp Val Asn Pro Asp Gln Arg Asp Phe Gln His Leu           
305                 310                 315                 320           

atc ctg ccc aac ggc aag ttc aac cca ttc acc gag tgc aac ggc ctg       1008
Ile Leu Pro Asn Gly Lys Phe Asn Pro Phe Thr Glu Cys Asn Gly Leu           
                325                 330                 335               

aac agg atc gtg gac gga tgc gtg cca gga ttc gtg ctg agg gtg gga       1056
Asn Arg Ile Val Asp Gly Cys Val Pro Gly Phe Val Leu Arg Val Gly           
            340                 345                 350                   

agg ggc aag gcc gtg aac cgg acc atc gtg acc ccc tac ctg aag cca       1104
Arg Gly Lys Ala Val Asn Arg Thr Ile Val Thr Pro Tyr Leu Lys Pro           
        355                 360                 365                       

tac gag tgc ttc ggc tgg tcc tgg aac gac aac cag gac agc atc tac       1152
Tyr Glu Cys Phe Gly Trp Ser Trp Asn Asp Asn Gln Asp Ser Ile Tyr           
    370                 375                 380                           

gac tgg tgg atc gcc gac ttc gtg tcc acc gga gcc ttc gtg tgc gag       1200
Asp Trp Trp Ile Ala Asp Phe Val Ser Thr Gly Ala Phe Val Cys Glu           
385                 390                 395                 400           

agc aac cca gag gct cca aag acc ggc gtg tgc gtg acc tac acc gtg       1248
Ser Asn Pro Glu Ala Pro Lys Thr Gly Val Cys Val Thr Tyr Thr Val           
                405                 410                 415               

gag aag gtg acc ttc cag ggc gtg ctg tac gag tcc aac ttc acc ttc       1296
Glu Lys Val Thr Phe Gln Gly Val Leu Tyr Glu Ser Asn Phe Thr Phe           
            420                 425                 430                   

gcc cag tac tac aac ctg ctg tac gtg ggc agc cag ctg aga tac gtg       1344
Ala Gln Tyr Tyr Asn Leu Leu Tyr Val Gly Ser Gln Leu Arg Tyr Val           
        435                 440                 445                       

aga atc ctg ggc aag gtg tac gag gtg tcc agc tgt ttc gag gcc agc       1392
Arg Ile Leu Gly Lys Val Tyr Glu Val Ser Ser Cys Phe Glu Ala Ser           
    450                 455                 460                           

tac gac gtg ctg tac cgc aac aac cag tcc ttc ggc ctg ctg tac cgg       1440
Tyr Asp Val Leu Tyr Arg Asn Asn Gln Ser Phe Gly Leu Leu Tyr Arg           
465                 470                 475                 480           

agc ttc gac tgc aac cag ctg cac atc aag tcc gcc aga ttc gtg gac       1488
Ser Phe Asp Cys Asn Gln Leu His Ile Lys Ser Ala Arg Phe Val Asp           
                485                 490                 495               

agg ctg ctg cca agc cac aac gga acc gcc acc gtg ctg ggc tgc ctg       1536
Arg Leu Leu Pro Ser His Asn Gly Thr Ala Thr Val Leu Gly Cys Leu           
            500                 505                 510                   

ttc aac gcc tcc tac gcc ccc aac gac acc atg gtg aac tgc acc aac       1584
Phe Asn Ala Ser Tyr Ala Pro Asn Asp Thr Met Val Asn Cys Thr Asn           
        515                 520                 525                       

cca ctg ggc gac gga ttc tgc gct gac ctg ctg gga aac gtg gcc gtg       1632
Pro Leu Gly Asp Gly Phe Cys Ala Asp Leu Leu Gly Asn Val Ala Val           
    530                 535                 540                           

cgc agg atg acc ttc gag aag cac gac acc acc tac gtg gcc ccc gtg       1680
Arg Arg Met Thr Phe Glu Lys His Asp Thr Thr Tyr Val Ala Pro Val           
545                 550                 555                 560           

acc aac gag agg tac acc gag atg cca ctg gac cac cag ctg atc ctg       1728
Thr Asn Glu Arg Tyr Thr Glu Met Pro Leu Asp His Gln Leu Ile Leu           
                565                 570                 575               

acc gag cag ttc ctg cag acc acc atg cca aag ttc tcc gtg agc tgc       1776
Thr Glu Gln Phe Leu Gln Thr Thr Met Pro Lys Phe Ser Val Ser Cys           
            580                 585                 590                   

gag aca tac atc tgc gac gtg agc aag gcc tgc aag aac ctg ctg ttc       1824
Glu Thr Tyr Ile Cys Asp Val Ser Lys Ala Cys Lys Asn Leu Leu Phe           
        595                 600                 605                       

agg tac ggc ggc ttc tgc cag aag gtg gag gcc gac atc aga gga gct       1872
Arg Tyr Gly Gly Phe Cys Gln Lys Val Glu Ala Asp Ile Arg Gly Ala           
    610                 615                 620                           

ggc atc ctg ctg gac ggc gac gtg tcc agc ctg tac tcc acc atc gct       1920
Gly Ile Leu Leu Asp Gly Asp Val Ser Ser Leu Tyr Ser Thr Ile Ala           
625                 630                 635                 640           

gct aag acc tcc agc gtg gtg cca acc acc gac agg ttc aac gtg agc       1968
Ala Lys Thr Ser Ser Val Val Pro Thr Thr Asp Arg Phe Asn Val Ser           
                645                 650                 655               

cag ttc ttc ctg cca aag acc cag tcc agc gcc aac aag tac gag tcc       2016
Gln Phe Phe Leu Pro Lys Thr Gln Ser Ser Ala Asn Lys Tyr Glu Ser           
            660                 665                 670                   

aga agc gcc atc gag gac ctg ctg ttc tcc aag atc gag aca acc ggc       2064
Arg Ser Ala Ile Glu Asp Leu Leu Phe Ser Lys Ile Glu Thr Thr Gly           
        675                 680                 685                       

ccc ggc ttc tac ggc gac tac tac aac tgc aag aag aac gcc atc cag       2112
Pro Gly Phe Tyr Gly Asp Tyr Tyr Asn Cys Lys Lys Asn Ala Ile Gln           
    690                 695                 700                           

gac ctg acc tgc gcc cag tac cac aac ggc atc ctg gtc atc ccc cca       2160
Asp Leu Thr Cys Ala Gln Tyr His Asn Gly Ile Leu Val Ile Pro Pro           
705                 710                 715                 720           

atc atg gac gcc gag aca ctg gga atg tac gga gga atc gct gcc gcc       2208
Ile Met Asp Ala Glu Thr Leu Gly Met Tyr Gly Gly Ile Ala Ala Ala           
                725                 730                 735               

tcc gtg acc ctg gga atc ttc gga gga cag gct gga atg gct acc tgg       2256
Ser Val Thr Leu Gly Ile Phe Gly Gly Gln Ala Gly Met Ala Thr Trp           
            740                 745                 750                   

agc gtg gcc atg gcc ggc cgg ctg aac gct ctg gga gtg gtg cag aac       2304
Ser Val Ala Met Ala Gly Arg Leu Asn Ala Leu Gly Val Val Gln Asn           
        755                 760                 765                       

gct ctg gtg gac gac gtg aac aag ctg gcc aac ggc ttc aac cag ctg       2352
Ala Leu Val Asp Asp Val Asn Lys Leu Ala Asn Gly Phe Asn Gln Leu           
    770                 775                 780                           

acc gcc tcc gtg agc aag ctg gcc ctg acc acc tcc agc gcc ctg cag       2400
Thr Ala Ser Val Ser Lys Leu Ala Leu Thr Thr Ser Ser Ala Leu Gln           
785                 790                 795                 800           

gct atc cag gct gtg gtg aac cag aac gct gct cag gtg gag tcc ctg       2448
Ala Ile Gln Ala Val Val Asn Gln Asn Ala Ala Gln Val Glu Ser Leu           
                805                 810                 815               

gtg agc ggc atc acc gag aac ttc ggc gcc atc tcc acc aac ttc aaa       2496
Val Ser Gly Ile Thr Glu Asn Phe Gly Ala Ile Ser Thr Asn Phe Lys           
            820                 825                 830                   

gtg atc agc cag cgc ctg gac aag ctg gag gct gac gtg cag atg gac       2544
Val Ile Ser Gln Arg Leu Asp Lys Leu Glu Ala Asp Val Gln Met Asp           
        835                 840                 845                       

cgc ctg atc aac ggc cgg atg aac gtg ctg cag ctg ttc gtg acc aac       2592
Arg Leu Ile Asn Gly Arg Met Asn Val Leu Gln Leu Phe Val Thr Asn           
    850                 855                 860                           

tac aag ctg aag atc gcc gag ctg agg aac acc cac aga tac gtg cag       2640
Tyr Lys Leu Lys Ile Ala Glu Leu Arg Asn Thr His Arg Tyr Val Gln           
865                 870                 875                 880           

tcc ctg atc aac gag tgc gtg tac gct cag agc ctg agg aac gga ttc       2688
Ser Leu Ile Asn Glu Cys Val Tyr Ala Gln Ser Leu Arg Asn Gly Phe           
                885                 890                 895               

tgc gga cag ggc ctg cac gtg ctg tcc ctg atg cag aac gcc ccc agc       2736
Cys Gly Gln Gly Leu His Val Leu Ser Leu Met Gln Asn Ala Pro Ser           
            900                 905                 910                   

ggc atc atg ttc ttc cac tac agc ctg atc ccc aac aac acc atc acc       2784
Gly Ile Met Phe Phe His Tyr Ser Leu Ile Pro Asn Asn Thr Ile Thr           
        915                 920                 925                       

gtg aag acc acc cca ggc ctg tgc gag tcc gac gag ctg gga agc aag       2832
Val Lys Thr Thr Pro Gly Leu Cys Glu Ser Asp Glu Leu Gly Ser Lys           
    930                 935                 940                           

tgc atc gtg gct aag gac ggc gtg ctg gtg tcc gcc aac ctg agc tac       2880
Cys Ile Val Ala Lys Asp Gly Val Leu Val Ser Ala Asn Leu Ser Tyr           
945                 950                 955                 960           

tgg cag tgg tcc ccc cgc aac ctg tac aag cca gag aac ctg acc ttc       2928
Trp Gln Trp Ser Pro Arg Asn Leu Tyr Lys Pro Glu Asn Leu Thr Phe           
                965                 970                 975               

gcc aac gtg atc gcc gtg agc agg gga gct aac tac acc acc ctg aac       2976
Ala Asn Val Ile Ala Val Ser Arg Gly Ala Asn Tyr Thr Thr Leu Asn           
            980                 985                 990                   

aag acc ttc gac atc ccc gag ctg  aac tcc acc ttc cca  atc gag gag     3024
Lys Thr Phe Asp Ile Pro Glu Leu  Asn Ser Thr Phe Pro  Ile Glu Glu         
        995                 1000                 1005                     

gag ttc  cgc gag tac ttc cag  aac atg tcc agc gag  ctg cag gtg        3069
Glu Phe  Arg Glu Tyr Phe Gln  Asn Met Ser Ser Glu  Leu Gln Val            
    1010                 1015                 1020                        

ctg aag  aac ctg acc gcc gac  atg tcc aag ctg aac  atc agc gcc        3114
Leu Lys  Asn Leu Thr Ala Asp  Met Ser Lys Leu Asn  Ile Ser Ala            
    1025                 1030                 1035                        

gag atc  cag ctg atc aac gag  atc gcc cac aac gtg  tcc aac atg        3159
Glu Ile  Gln Leu Ile Asn Glu  Ile Ala His Asn Val  Ser Asn Met            
    1040                 1045                 1050                        

agg gtg  gag gtg gag aag ttc  cag aga tac gtg aac  tac gtg aag        3204
Arg Val  Glu Val Glu Lys Phe  Gln Arg Tyr Val Asn  Tyr Val Lys            
    1055                 1060                 1065                        

tgg gcc  tgg tgg cag tgg ctg  ctg atc ttc atc gcc  ctg acc ctg        3249
Trp Ala  Trp Trp Gln Trp Leu  Leu Ile Phe Ile Ala  Leu Thr Leu            
    1070                 1075                 1080                        

ctg gct  ggc ctg atg ctg tgg  tgc tgc ctg gcc acc  ggc tgc tgc        3294
Leu Ala  Gly Leu Met Leu Trp  Cys Cys Leu Ala Thr  Gly Cys Cys            
    1085                 1090                 1095                        

ggc atg  tgc gga tgc ctg gct  gct acc tgc gct tcc  tgc tgc gac        3339
Gly Met  Cys Gly Cys Leu Ala  Ala Thr Cys Ala Ser  Cys Cys Asp            
    1100                 1105                 1110                        

tgc agg  gga acc aag ctg cag  agc tac gag atc gag  aag gtc cat        3384
Cys Arg  Gly Thr Lys Leu Gln  Ser Tyr Glu Ile Glu  Lys Val His            
    1115                 1120                 1125                        

gtc cag                                                               3390
Val Gln                                                                   
    1130                                                                  


<210>  19
<211>  1130
<212>  PRT
<213>  porcine enteric alphacoronavirus

<400>  19

Met Lys Leu Phe Thr Val Phe Thr Leu Leu Ala Ser Ile Arg Val Leu 
1               5                   10                  15      


Tyr Gly Cys Glu Ser Val Asp Phe Asn Leu Phe Asn Thr Ile Phe Ser 
            20                  25                  30          


Thr His Arg Gly Leu Ser Asn Thr Thr Ser Val Ile Thr Gly Ala Tyr 
        35                  40                  45              


Pro Ser Thr Asn Lys Ser Asp Trp Ser Cys Asn Thr Arg Thr Gly His 
    50                  55                  60                  


Leu Ser Gly Ser Gly Phe Gly Ile Gly Leu Tyr Val Gln Thr Pro Arg 
65                  70                  75                  80  


Glu Gln Tyr Gln Tyr Asp Gly Ser Gly Ala Gly Gly Tyr Thr Ile Ala 
                85                  90                  95      


Val Ser Pro Ile His Val Thr Asn Leu Thr Trp Glu Leu Trp Ile His 
            100                 105                 110         


Arg Lys Trp Gly Val Asn Ser Val Val Thr Val Arg Leu Cys Arg Trp 
        115                 120                 125             


Trp Gln Phe Met Ser Phe Asn Ser Thr Ser His Ala Ala Asp Ala Gly 
    130                 135                 140                 


Pro Thr Asn Ala Phe Glu Cys Leu Ile Asn Gly Ser Tyr Pro Thr His 
145                 150                 155                 160 


Arg Asn Thr Gly Tyr Met Phe Gly Val Thr Trp Tyr Asn Asp Leu Val 
                165                 170                 175     


Arg Ile Val Phe Pro Pro Thr Val Leu Glu Met Gln Leu Asp Gly Leu 
            180                 185                 190         


Gln Trp Glu Arg Val Gln Phe Asn Ser Pro Val Asn Ala Gly His Ala 
        195                 200                 205             


Thr Arg Phe Asn Val Val Lys Asp Ile Ser Thr Val Leu Val Glu Thr 
    210                 215                 220                 


Asn Ser Gly Gly Ser Val Phe Arg Tyr Ser Tyr Cys Ala Asp Gly Phe 
225                 230                 235                 240 


Val Asn Gly Leu Gln Cys Lys Leu Arg Leu Phe Asp Ile Pro Pro Gly 
                245                 250                 255     


Val Tyr Ser Asn Ser Glu Val Glu Tyr Pro Thr Ala Leu Tyr Thr Val 
            260                 265                 270         


Val His Asn Met Ser Ala Cys Pro Glu Arg Pro Asp Ser Tyr Cys Gly 
        275                 280                 285             


Ser Asn Ser Cys Pro Phe Lys Arg Ala Val Phe Ser Asn Cys Ile Val 
    290                 295                 300                 


Asn Tyr Thr Thr Trp Val Asn Pro Asp Gln Arg Asp Phe Gln His Leu 
305                 310                 315                 320 


Ile Leu Pro Asn Gly Lys Phe Asn Pro Phe Thr Glu Cys Asn Gly Leu 
                325                 330                 335     


Asn Arg Ile Val Asp Gly Cys Val Pro Gly Phe Val Leu Arg Val Gly 
            340                 345                 350         


Arg Gly Lys Ala Val Asn Arg Thr Ile Val Thr Pro Tyr Leu Lys Pro 
        355                 360                 365             


Tyr Glu Cys Phe Gly Trp Ser Trp Asn Asp Asn Gln Asp Ser Ile Tyr 
    370                 375                 380                 


Asp Trp Trp Ile Ala Asp Phe Val Ser Thr Gly Ala Phe Val Cys Glu 
385                 390                 395                 400 


Ser Asn Pro Glu Ala Pro Lys Thr Gly Val Cys Val Thr Tyr Thr Val 
                405                 410                 415     


Glu Lys Val Thr Phe Gln Gly Val Leu Tyr Glu Ser Asn Phe Thr Phe 
            420                 425                 430         


Ala Gln Tyr Tyr Asn Leu Leu Tyr Val Gly Ser Gln Leu Arg Tyr Val 
        435                 440                 445             


Arg Ile Leu Gly Lys Val Tyr Glu Val Ser Ser Cys Phe Glu Ala Ser 
    450                 455                 460                 


Tyr Asp Val Leu Tyr Arg Asn Asn Gln Ser Phe Gly Leu Leu Tyr Arg 
465                 470                 475                 480 


Ser Phe Asp Cys Asn Gln Leu His Ile Lys Ser Ala Arg Phe Val Asp 
                485                 490                 495     


Arg Leu Leu Pro Ser His Asn Gly Thr Ala Thr Val Leu Gly Cys Leu 
            500                 505                 510         


Phe Asn Ala Ser Tyr Ala Pro Asn Asp Thr Met Val Asn Cys Thr Asn 
        515                 520                 525             


Pro Leu Gly Asp Gly Phe Cys Ala Asp Leu Leu Gly Asn Val Ala Val 
    530                 535                 540                 


Arg Arg Met Thr Phe Glu Lys His Asp Thr Thr Tyr Val Ala Pro Val 
545                 550                 555                 560 


Thr Asn Glu Arg Tyr Thr Glu Met Pro Leu Asp His Gln Leu Ile Leu 
                565                 570                 575     


Thr Glu Gln Phe Leu Gln Thr Thr Met Pro Lys Phe Ser Val Ser Cys 
            580                 585                 590         


Glu Thr Tyr Ile Cys Asp Val Ser Lys Ala Cys Lys Asn Leu Leu Phe 
        595                 600                 605             


Arg Tyr Gly Gly Phe Cys Gln Lys Val Glu Ala Asp Ile Arg Gly Ala 
    610                 615                 620                 


Gly Ile Leu Leu Asp Gly Asp Val Ser Ser Leu Tyr Ser Thr Ile Ala 
625                 630                 635                 640 


Ala Lys Thr Ser Ser Val Val Pro Thr Thr Asp Arg Phe Asn Val Ser 
                645                 650                 655     


Gln Phe Phe Leu Pro Lys Thr Gln Ser Ser Ala Asn Lys Tyr Glu Ser 
            660                 665                 670         


Arg Ser Ala Ile Glu Asp Leu Leu Phe Ser Lys Ile Glu Thr Thr Gly 
        675                 680                 685             


Pro Gly Phe Tyr Gly Asp Tyr Tyr Asn Cys Lys Lys Asn Ala Ile Gln 
    690                 695                 700                 


Asp Leu Thr Cys Ala Gln Tyr His Asn Gly Ile Leu Val Ile Pro Pro 
705                 710                 715                 720 


Ile Met Asp Ala Glu Thr Leu Gly Met Tyr Gly Gly Ile Ala Ala Ala 
                725                 730                 735     


Ser Val Thr Leu Gly Ile Phe Gly Gly Gln Ala Gly Met Ala Thr Trp 
            740                 745                 750         


Ser Val Ala Met Ala Gly Arg Leu Asn Ala Leu Gly Val Val Gln Asn 
        755                 760                 765             


Ala Leu Val Asp Asp Val Asn Lys Leu Ala Asn Gly Phe Asn Gln Leu 
    770                 775                 780                 


Thr Ala Ser Val Ser Lys Leu Ala Leu Thr Thr Ser Ser Ala Leu Gln 
785                 790                 795                 800 


Ala Ile Gln Ala Val Val Asn Gln Asn Ala Ala Gln Val Glu Ser Leu 
                805                 810                 815     


Val Ser Gly Ile Thr Glu Asn Phe Gly Ala Ile Ser Thr Asn Phe Lys 
            820                 825                 830         


Val Ile Ser Gln Arg Leu Asp Lys Leu Glu Ala Asp Val Gln Met Asp 
        835                 840                 845             


Arg Leu Ile Asn Gly Arg Met Asn Val Leu Gln Leu Phe Val Thr Asn 
    850                 855                 860                 


Tyr Lys Leu Lys Ile Ala Glu Leu Arg Asn Thr His Arg Tyr Val Gln 
865                 870                 875                 880 


Ser Leu Ile Asn Glu Cys Val Tyr Ala Gln Ser Leu Arg Asn Gly Phe 
                885                 890                 895     


Cys Gly Gln Gly Leu His Val Leu Ser Leu Met Gln Asn Ala Pro Ser 
            900                 905                 910         


Gly Ile Met Phe Phe His Tyr Ser Leu Ile Pro Asn Asn Thr Ile Thr 
        915                 920                 925             


Val Lys Thr Thr Pro Gly Leu Cys Glu Ser Asp Glu Leu Gly Ser Lys 
    930                 935                 940                 


Cys Ile Val Ala Lys Asp Gly Val Leu Val Ser Ala Asn Leu Ser Tyr 
945                 950                 955                 960 


Trp Gln Trp Ser Pro Arg Asn Leu Tyr Lys Pro Glu Asn Leu Thr Phe 
                965                 970                 975     


Ala Asn Val Ile Ala Val Ser Arg Gly Ala Asn Tyr Thr Thr Leu Asn 
            980                 985                 990         


Lys Thr Phe Asp Ile Pro Glu Leu  Asn Ser Thr Phe Pro  Ile Glu Glu 
        995                 1000                 1005             


Glu Phe  Arg Glu Tyr Phe Gln  Asn Met Ser Ser Glu  Leu Gln Val 
    1010                 1015                 1020             


Leu Lys  Asn Leu Thr Ala Asp  Met Ser Lys Leu Asn  Ile Ser Ala 
    1025                 1030                 1035             


Glu Ile  Gln Leu Ile Asn Glu  Ile Ala His Asn Val  Ser Asn Met 
    1040                 1045                 1050             


Arg Val  Glu Val Glu Lys Phe  Gln Arg Tyr Val Asn  Tyr Val Lys 
    1055                 1060                 1065             


Trp Ala  Trp Trp Gln Trp Leu  Leu Ile Phe Ile Ala  Leu Thr Leu 
    1070                 1075                 1080             


Leu Ala  Gly Leu Met Leu Trp  Cys Cys Leu Ala Thr  Gly Cys Cys 
    1085                 1090                 1095             


Gly Met  Cys Gly Cys Leu Ala  Ala Thr Cys Ala Ser  Cys Cys Asp 
    1100                 1105                 1110             


Cys Arg  Gly Thr Lys Leu Gln  Ser Tyr Glu Ile Glu  Lys Val His 
    1115                 1120                 1125             


Val Gln  
    1130 


<210>  20
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCoV consensus Spike with furin site mutation

<400>  20
atgtttctga ttctgctgat tagcctgcca accgcttttg ctgtcatcgg ggacctgaaa       60

tgcaccaccg tgagtattaa cgacgtggac acaggcgtgc cttccatcag caccgacacc      120

gtggacgtga ccaacggcct gggcacctac tacgtgctgg accgcgtgta cctgaacacc      180

accctgctgc tgaacggcta ctaccctacc tccggcagca cctacagaaa catggccctg      240

aagggcaccc tgctgctgtc caccctgtgg ttcaagcccc ctttcctgag cgacttcacc      300

aacggcatct tcgccaaggt gaagaacacc aaagtgatca aggacggcgt gatgtactcc      360

gagttccccg ccatcaccat cggcagcacc ttcgtgaaca cctcctacag cgtggtggtg      420

cagcctcaca ccaccatcct gggcaacaag ctgcagggct tcctggagat ctccgtgtgc      480

cagtacacca tgtgcgagta ccccaacacc atctgcaacc ctaacctggg caaccagagg      540

gtggagctgt ggcactggga cacaggcgtg gtgagctgcc tgtacaagag gaacttcacc      600

tacgacgtga acgccgacta cctgtacttc cacttctacc aggagggcgg caccttctac      660

gcctacttca ccgacacagg cgtggtgacc aagttcctgt tcaacgtgta cctgggcacc      720

gtgctgtccc actactacgt gatgcccctg acctgcaaca gcgccctgac cctggagtac      780

tgggtgaccc ctctgaccag caagcagtac ctgctggcct tcaaccagga cggcgtgatc      840

ttcaacgccg tggactgcaa gtccgacttc atgagcgaga tcaagtgcaa gaccctgtcc      900

atcgctccta gcaccggggt gtacgagctg aacggctaca ccgtgcagcc tatcgccgac      960

gtgtaccgga ggatccccaa cctgcctgac tgcaacatcg aggcctggct gaacgacaag     1020

tccgtgccca gccctctgaa ctgggagcgg aagaccttca gcaactgcaa cttcaacatg     1080

agctccctga tgtccttcat ccaggccgac agcttcacct gcaacaacat cgacgccgcc     1140

aagatctacg gcatgtgctt cagctccatc accatcgaca agttcgccat ccccaacggc     1200

cggaaggtgg acctgcagct gggcaacctg ggctacctgc agtccttcaa ctacaggatc     1260

gacaccaccg ccaccagctg ccagctgtac tacaacctgc ctgctgctaa cgtgtccgtg     1320

agcaggttca accctagcac ctggaaccgc agattcggct tcaccgagca gtccgtgttc     1380

aagccccagc ctgccggcgt gttcaccgac cacgacgtgg tgtacgccca gcactgcttc     1440

aaggccccca ccaacttctg cccttgcaag ctggacggca gcctgtgcgt gggatccgga     1500

agcggcatcg acgccggcta caagaacacc ggcatcggaa cctgccctgc tggaaccaac     1560

tacctgacct gccacaacgc tgctcagtgc gactgcctgt gcacccccga ccctatcacc     1620

tccaaggcca ccggccccta caagtgccct cagaccaagt acctggtggg aatcggagag     1680

cactgctccg gcctggccat caagagcgac cactgcggag gaaacccttg cagctgccag     1740

cctcaggcct tcctgggatg gtccgtggac agctgcctgc agggcgaccg ctgcaacatc     1800

ttcgccaact tcatcctgca cgacgtgaac tccggcacca cctgcagcac cgacctgcag     1860

aagagcaaca ccgacatcat cctgggcgtg tgcgtgaact acgacctgta cggcatcacc     1920

ggccagggca tcttcgtgga ggtgaacgcc acctactaca actcctggca gaacctgctg     1980

tacgacagca acggcaacct gtacggcttc cgcgactacc tgaccaaccg gaccttcatg     2040

atcaggtcct gctacagcgg cagagtgagc gccgctttcc acgccaacag ctccgagccc     2100

gccctgctgt tccggaacat caagtgcaac tacgtgttca acaacaccct gtccaggcag     2160

ctgcagccta tcaactactt cgacagctac ctgggctgcg tggtgaacgc cgacaactcc     2220

accagctccg tggtgcagac ctgcgacctg accgtgggat ccggatactg cgtggactac     2280

agcaccaagg ccgcctccgc cgccagcatc accaccggct acagattcac caacttcgag     2340

cccttcaccg tgaactccgt gaacgacagc ctggagcccg tgggaggcct gtacgagatc     2400

cagatcccta gcgagttcac catcggcaac atggaggagt tcatccagac cagctcccct     2460

aaggtgacca tcgactgctc cgccttcgtg tgcggcgact acgctgcttg caagtcccag     2520

ctggtggagt acggcagctt ctgcgacaac atcaacgcca tcctgaccga ggtgaacgag     2580

ctgctggaca ccacccagct gcaggtggcc aactccctga tgaacggcgt gaccctgagc     2640

accaagctga aggacggcgt gaacttcaac gtggacgata tcaacttctc ccccgtgctg     2700

ggatgcctgg gaagcgactg caacaaggtg agctcccggt ccgccatcga ggacctgctg     2760

ttctccaagg tgaagctgag cgacgtgggc ttcgtggagg cctacaacaa ctgcacaggc     2820

ggagctgaga tcagggacct gatctgcgtg cagagctaca acggcatcaa ggtgctgccc     2880

cctctgctgt ccgagaacca gatcagcggc tacaccctgg ctgccacctc cgccagcctg     2940

ttccctcctt ggagcgccgc cgccggggtg cccttctacc tgaacgtgca gtaccgcatc     3000

aacggcatcg gcgtgaccat ggacgtgctg agccagaacc agaagctgat cgccaacgcc     3060

ttcaacaacg ctctgggagc tatccaggag ggattcgacg ctaccaacag cgccctggtg     3120

aagatccagg ctgtggtgaa cgctaacgct gaggccctga acaacctgct gcagcagctg     3180

agcaaccgct tcggcgccat cagctccagc ctgcaggaga tcctgtccag actggacgcc     3240

ctggaggctc aggctcagat cgaccgcctg atcaacggca gactgaccgc cctgaacgcc     3300

tacgtgagcc agcagctgtc cgacagcacc ctggtgaagt tctccgccgc ccaggccatg     3360

gagaaggtga acgagtgcgt gaagagccag tccagccgca tcaacttctg cggcaacggc     3420

aaccacatca tctccctggt gcagaacgcc ccctacggcc tgtacttcat ccacttcagc     3480

tacgtgccca ccaagtacgt gaccgccaag gtgtcccctg gcctgtgcat cgccggggac     3540

cggggcatcg cccctaagag cggctacttc gtgaacgtga acaacacctg gatgttcacc     3600

ggctccggct actactaccc cgagcctatc accggcaaca acgtggtggt catgagcacc     3660

tgcgccgtga actacaccaa ggcccccgac gtgatgctga acatctccac ccccaacctg     3720

cctgacttca aggaggagct ggaccagtgg ttcaagaacc agacctccgt ggcccctgac     3780

ctgagcctgg actacatcaa cgtgaccttc ctggacctgc aggacgagat gaacaggctg     3840

caggaggcca tcaaggtgct gaaccagtcc tacatcaacc tgaaggacat cggcacctac     3900

gagtactacg tgaagtggcc ctggtacgtg tggctgctga tcggctttgc cggcgtggcc     3960

atgctggtgc tgctgttctt catctgctgc tgcaccggct gcggcaccag ctgcttcaag     4020

aagtgcggcg gctgctgcga cgattacact ggacatcagg aactggtcat caaaacatca     4080

cacgaggact aa                                                         4092


<210>  21
<211>  4092
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCoV consensus Spike with mutated furin site and 2P mutation

<400>  21
atgtttctga ttctgctgat tagcctgcca accgcttttg ctgtcatcgg ggacctgaaa       60

tgcaccaccg tgagtattaa cgacgtggac acaggcgtgc cttccatcag caccgacacc      120

gtggacgtga ccaacggcct gggcacctac tacgtgctgg accgcgtgta cctgaacacc      180

accctgctgc tgaacggcta ctaccctacc tccggcagca cctacagaaa catggccctg      240

aagggcaccc tgctgctgtc caccctgtgg ttcaagcccc ctttcctgag cgacttcacc      300

aacggcatct tcgccaaggt gaagaacacc aaagtgatca aggacggcgt gatgtactcc      360

gagttccccg ccatcaccat cggcagcacc ttcgtgaaca cctcctacag cgtggtggtg      420

cagcctcaca ccaccatcct gggcaacaag ctgcagggct tcctggagat ctccgtgtgc      480

cagtacacca tgtgcgagta ccccaacacc atctgcaacc ctaacctggg caaccagagg      540

gtggagctgt ggcactggga cacaggcgtg gtgagctgcc tgtacaagag gaacttcacc      600

tacgacgtga acgccgacta cctgtacttc cacttctacc aggagggcgg caccttctac      660

gcctacttca ccgacacagg cgtggtgacc aagttcctgt tcaacgtgta cctgggcacc      720

gtgctgtccc actactacgt gatgcccctg acctgcaaca gcgccctgac cctggagtac      780

tgggtgaccc ctctgaccag caagcagtac ctgctggcct tcaaccagga cggcgtgatc      840

ttcaacgccg tggactgcaa gtccgacttc atgagcgaga tcaagtgcaa gaccctgtcc      900

atcgctccta gcaccggggt gtacgagctg aacggctaca ccgtgcagcc tatcgccgac      960

gtgtaccgga ggatccccaa cctgcctgac tgcaacatcg aggcctggct gaacgacaag     1020

tccgtgccca gccctctgaa ctgggagcgg aagaccttca gcaactgcaa cttcaacatg     1080

agctccctga tgtccttcat ccaggccgac agcttcacct gcaacaacat cgacgccgcc     1140

aagatctacg gcatgtgctt cagctccatc accatcgaca agttcgccat ccccaacggc     1200

cggaaggtgg acctgcagct gggcaacctg ggctacctgc agtccttcaa ctacaggatc     1260

gacaccaccg ccaccagctg ccagctgtac tacaacctgc ctgctgctaa cgtgtccgtg     1320

agcaggttca accctagcac ctggaaccgc agattcggct tcaccgagca gtccgtgttc     1380

aagccccagc ctgccggcgt gttcaccgac cacgacgtgg tgtacgccca gcactgcttc     1440

aaggccccca ccaacttctg cccttgcaag ctggacggca gcctgtgcgt gggatccgga     1500

agcggcatcg acgccggcta caagaacacc ggcatcggaa cctgccctgc tggaaccaac     1560

tacctgacct gccacaacgc tgctcagtgc gactgcctgt gcacccccga ccctatcacc     1620

tccaaggcca ccggccccta caagtgccct cagaccaagt acctggtggg aatcggagag     1680

cactgctccg gcctggccat caagagcgac cactgcggag gaaacccttg cagctgccag     1740

cctcaggcct tcctgggatg gtccgtggac agctgcctgc agggcgaccg ctgcaacatc     1800

ttcgccaact tcatcctgca cgacgtgaac tccggcacca cctgcagcac cgacctgcag     1860

aagagcaaca ccgacatcat cctgggcgtg tgcgtgaact acgacctgta cggcatcacc     1920

ggccagggca tcttcgtgga ggtgaacgcc acctactaca actcctggca gaacctgctg     1980

tacgacagca acggcaacct gtacggcttc cgcgactacc tgaccaaccg gaccttcatg     2040

atcaggtcct gctacagcgg cagagtgagc gccgctttcc acgccaacag ctccgagccc     2100

gccctgctgt tccggaacat caagtgcaac tacgtgttca acaacaccct gtccaggcag     2160

ctgcagccta tcaactactt cgacagctac ctgggctgcg tggtgaacgc cgacaactcc     2220

accagctccg tggtgcagac ctgcgacctg accgtgggat ccggatactg cgtggactac     2280

agcaccaagg ccgcctccgc cgccagcatc accaccggct acagattcac caacttcgag     2340

cccttcaccg tgaactccgt gaacgacagc ctggagcccg tgggaggcct gtacgagatc     2400

cagatcccta gcgagttcac catcggcaac atggaggagt tcatccagac cagctcccct     2460

aaggtgacca tcgactgctc cgccttcgtg tgcggcgact acgctgcttg caagtcccag     2520

ctggtggagt acggcagctt ctgcgacaac atcaacgcca tcctgaccga ggtgaacgag     2580

ctgctggaca ccacccagct gcaggtggcc aactccctga tgaacggcgt gaccctgagc     2640

accaagctga aggacggcgt gaacttcaac gtggacgata tcaacttctc ccccgtgctg     2700

ggatgcctgg gaagcgactg caacaaggtg agctcccggt ccgccatcga ggacctgctg     2760

ttctccaagg tgaagctgag cgacgtgggc ttcgtggagg cctacaacaa ctgcacaggc     2820

ggagctgaga tcagggacct gatctgcgtg cagagctaca acggcatcaa ggtgctgccc     2880

cctctgctgt ccgagaacca gatcagcggc tacaccctgg ctgccacctc cgccagcctg     2940

ttccctcctt ggagcgccgc cgccggggtg cccttctacc tgaacgtgca gtaccgcatc     3000

aacggcatcg gcgtgaccat ggacgtgctg agccagaacc agaagctgat cgccaacgcc     3060

ttcaacaacg ctctgggagc tatccaggag ggattcgacg ctaccaacag cgccctggtg     3120

aagatccagg ctgtggtgaa cgctaacgct gaggccctga acaacctgct gcagcagctg     3180

agcaaccgct tcggcgccat cagctccagc ctgcaggaga tcctgtccag actggacccc     3240

cccgaggctc aggctcagat cgaccgcctg atcaacggca gactgaccgc cctgaacgcc     3300

tacgtgagcc agcagctgtc cgacagcacc ctggtgaagt tctccgccgc ccaggccatg     3360

gagaaggtga acgagtgcgt gaagagccag tccagccgca tcaacttctg cggcaacggc     3420

aaccacatca tctccctggt gcagaacgcc ccctacggcc tgtacttcat ccacttcagc     3480

tacgtgccca ccaagtacgt gaccgccaag gtgtcccctg gcctgtgcat cgccggggac     3540

cggggcatcg cccctaagag cggctacttc gtgaacgtga acaacacctg gatgttcacc     3600

ggctccggct actactaccc cgagcctatc accggcaaca acgtggtggt catgagcacc     3660

tgcgccgtga actacaccaa ggcccccgac gtgatgctga acatctccac ccccaacctg     3720

cctgacttca aggaggagct ggaccagtgg ttcaagaacc agacctccgt ggcccctgac     3780

ctgagcctgg actacatcaa cgtgaccttc ctggacctgc aggacgagat gaacaggctg     3840

caggaggcca tcaaggtgct gaaccagtcc tacatcaacc tgaaggacat cggcacctac     3900

gagtactacg tgaagtggcc ctggtacgtg tggctgctga tcggctttgc cggcgtggcc     3960

atgctggtgc tgctgttctt catctgctgc tgcaccggct gcggcaccag ctgcttcaag     4020

aagtgcggcg gctgctgcga cgattacact ggacatcagg aactggtcat caaaacatca     4080

cacgaggact aa                                                         4092


<210>  22
<211>  4032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCoV consensus Spike protein with furin site mutation and 
       Influenza virus HA gene TMD-CTD region

<400>  22
atgtttctga ttctgctgat tagcctgcca accgcttttg ctgtcatcgg ggacctgaaa       60

tgcaccaccg tgagtattaa cgacgtggac acaggcgtgc cttccatcag caccgacacc      120

gtggacgtga ccaacggcct gggcacctac tacgtgctgg accgcgtgta cctgaacacc      180

accctgctgc tgaacggcta ctaccctacc tccggcagca cctacagaaa catggccctg      240

aagggcaccc tgctgctgtc caccctgtgg ttcaagcccc ctttcctgag cgacttcacc      300

aacggcatct tcgccaaggt gaagaacacc aaagtgatca aggacggcgt gatgtactcc      360

gagttccccg ccatcaccat cggcagcacc ttcgtgaaca cctcctacag cgtggtggtg      420

cagcctcaca ccaccatcct gggcaacaag ctgcagggct tcctggagat ctccgtgtgc      480

cagtacacca tgtgcgagta ccccaacacc atctgcaacc ctaacctggg caaccagagg      540

gtggagctgt ggcactggga cacaggcgtg gtgagctgcc tgtacaagag gaacttcacc      600

tacgacgtga acgccgacta cctgtacttc cacttctacc aggagggcgg caccttctac      660

gcctacttca ccgacacagg cgtggtgacc aagttcctgt tcaacgtgta cctgggcacc      720

gtgctgtccc actactacgt gatgcccctg acctgcaaca gcgccctgac cctggagtac      780

tgggtgaccc ctctgaccag caagcagtac ctgctggcct tcaaccagga cggcgtgatc      840

ttcaacgccg tggactgcaa gtccgacttc atgagcgaga tcaagtgcaa gaccctgtcc      900

atcgctccta gcaccggggt gtacgagctg aacggctaca ccgtgcagcc tatcgccgac      960

gtgtaccgga ggatccccaa cctgcctgac tgcaacatcg aggcctggct gaacgacaag     1020

tccgtgccca gccctctgaa ctgggagcgg aagaccttca gcaactgcaa cttcaacatg     1080

agctccctga tgtccttcat ccaggccgac agcttcacct gcaacaacat cgacgccgcc     1140

aagatctacg gcatgtgctt cagctccatc accatcgaca agttcgccat ccccaacggc     1200

cggaaggtgg acctgcagct gggcaacctg ggctacctgc agtccttcaa ctacaggatc     1260

gacaccaccg ccaccagctg ccagctgtac tacaacctgc ctgctgctaa cgtgtccgtg     1320

agcaggttca accctagcac ctggaaccgc agattcggct tcaccgagca gtccgtgttc     1380

aagccccagc ctgccggcgt gttcaccgac cacgacgtgg tgtacgccca gcactgcttc     1440

aaggccccca ccaacttctg cccttgcaag ctggacggca gcctgtgcgt gggatccgga     1500

agcggcatcg acgccggcta caagaacacc ggcatcggaa cctgccctgc tggaaccaac     1560

tacctgacct gccacaacgc tgctcagtgc gactgcctgt gcacccccga ccctatcacc     1620

tccaaggcca ccggccccta caagtgccct cagaccaagt acctggtggg aatcggagag     1680

cactgctccg gcctggccat caagagcgac cactgcggag gaaacccttg cagctgccag     1740

cctcaggcct tcctgggatg gtccgtggac agctgcctgc agggcgaccg ctgcaacatc     1800

ttcgccaact tcatcctgca cgacgtgaac tccggcacca cctgcagcac cgacctgcag     1860

aagagcaaca ccgacatcat cctgggcgtg tgcgtgaact acgacctgta cggcatcacc     1920

ggccagggca tcttcgtgga ggtgaacgcc acctactaca actcctggca gaacctgctg     1980

tacgacagca acggcaacct gtacggcttc cgcgactacc tgaccaaccg gaccttcatg     2040

atcaggtcct gctacagcgg cagagtgagc gccgctttcc acgccaacag ctccgagccc     2100

gccctgctgt tccggaacat caagtgcaac tacgtgttca acaacaccct gtccaggcag     2160

ctgcagccta tcaactactt cgacagctac ctgggctgcg tggtgaacgc cgacaactcc     2220

accagctccg tggtgcagac ctgcgacctg accgtgggat ccggatactg cgtggactac     2280

agcaccaagg ccgcctccgc cgccagcatc accaccggct acagattcac caacttcgag     2340

cccttcaccg tgaactccgt gaacgacagc ctggagcccg tgggaggcct gtacgagatc     2400

cagatcccta gcgagttcac catcggcaac atggaggagt tcatccagac cagctcccct     2460

aaggtgacca tcgactgctc cgccttcgtg tgcggcgact acgctgcttg caagtcccag     2520

ctggtggagt acggcagctt ctgcgacaac atcaacgcca tcctgaccga ggtgaacgag     2580

ctgctggaca ccacccagct gcaggtggcc aactccctga tgaacggcgt gaccctgagc     2640

accaagctga aggacggcgt gaacttcaac gtggacgata tcaacttctc ccccgtgctg     2700

ggatgcctgg gaagcgactg caacaaggtg agctcccggt ccgccatcga ggacctgctg     2760

ttctccaagg tgaagctgag cgacgtgggc ttcgtggagg cctacaacaa ctgcacaggc     2820

ggagctgaga tcagggacct gatctgcgtg cagagctaca acggcatcaa ggtgctgccc     2880

cctctgctgt ccgagaacca gatcagcggc tacaccctgg ctgccacctc cgccagcctg     2940

ttccctcctt ggagcgccgc cgccggggtg cccttctacc tgaacgtgca gtaccgcatc     3000

aacggcatcg gcgtgaccat ggacgtgctg agccagaacc agaagctgat cgccaacgcc     3060

ttcaacaacg ctctgggagc tatccaggag ggattcgacg ctaccaacag cgccctggtg     3120

aagatccagg ctgtggtgaa cgctaacgct gaggccctga acaacctgct gcagcagctg     3180

agcaaccgct tcggcgccat cagctccagc ctgcaggaga tcctgtccag actggacgcc     3240

ctggaggctc aggctcagat cgaccgcctg atcaacggca gactgaccgc cctgaacgcc     3300

tacgtgagcc agcagctgtc cgacagcacc ctggtgaagt tctccgccgc ccaggccatg     3360

gagaaggtga acgagtgcgt gaagagccag tccagccgca tcaacttctg cggcaacggc     3420

aaccacatca tctccctggt gcagaacgcc ccctacggcc tgtacttcat ccacttcagc     3480

tacgtgccca ccaagtacgt gaccgccaag gtgtcccctg gcctgtgcat cgccggggac     3540

cggggcatcg cccctaagag cggctacttc gtgaacgtga acaacacctg gatgttcacc     3600

ggctccggct actactaccc cgagcctatc accggcaaca acgtggtggt catgagcacc     3660

tgcgccgtga actacaccaa ggcccccgac gtgatgctga acatctccac ccccaacctg     3720

cctgacttca aggaggagct ggaccagtgg ttcaagaacc agacctccgt ggcccctgac     3780

ctgagcctgg actacatcaa cgtgaccttc ctggacctgc aggacgagat gaacaggctg     3840

caggaggcca tcaaggtgct gaaccagtcc tacatcaacc tgaaggacat cggcacctac     3900

gagtactacg tgaagtggcc cctggccatc tacagcaccg tggctagctc cctggtgctg     3960

ctggtgagcc tgggagccat ctccttctgg atgtgcagca acggctccct gcagtgccgg     4020

atctgcatct aa                                                         4032


<210>  23
<211>  4071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BCoV consensus Spike protein with furin site mutation and VSV G 
       protein TMD-CTD region

<400>  23
atgtttctga ttctgctgat tagcctgcca accgcttttg ctgtcatcgg ggacctgaaa       60

tgcaccaccg tgagtattaa cgacgtggac acaggcgtgc cttccatcag caccgacacc      120

gtggacgtga ccaacggcct gggcacctac tacgtgctgg accgcgtgta cctgaacacc      180

accctgctgc tgaacggcta ctaccctacc tccggcagca cctacagaaa catggccctg      240

aagggcaccc tgctgctgtc caccctgtgg ttcaagcccc ctttcctgag cgacttcacc      300

aacggcatct tcgccaaggt gaagaacacc aaagtgatca aggacggcgt gatgtactcc      360

gagttccccg ccatcaccat cggcagcacc ttcgtgaaca cctcctacag cgtggtggtg      420

cagcctcaca ccaccatcct gggcaacaag ctgcagggct tcctggagat ctccgtgtgc      480

cagtacacca tgtgcgagta ccccaacacc atctgcaacc ctaacctggg caaccagagg      540

gtggagctgt ggcactggga cacaggcgtg gtgagctgcc tgtacaagag gaacttcacc      600

tacgacgtga acgccgacta cctgtacttc cacttctacc aggagggcgg caccttctac      660

gcctacttca ccgacacagg cgtggtgacc aagttcctgt tcaacgtgta cctgggcacc      720

gtgctgtccc actactacgt gatgcccctg acctgcaaca gcgccctgac cctggagtac      780

tgggtgaccc ctctgaccag caagcagtac ctgctggcct tcaaccagga cggcgtgatc      840

ttcaacgccg tggactgcaa gtccgacttc atgagcgaga tcaagtgcaa gaccctgtcc      900

atcgctccta gcaccggggt gtacgagctg aacggctaca ccgtgcagcc tatcgccgac      960

gtgtaccgga ggatccccaa cctgcctgac tgcaacatcg aggcctggct gaacgacaag     1020

tccgtgccca gccctctgaa ctgggagcgg aagaccttca gcaactgcaa cttcaacatg     1080

agctccctga tgtccttcat ccaggccgac agcttcacct gcaacaacat cgacgccgcc     1140

aagatctacg gcatgtgctt cagctccatc accatcgaca agttcgccat ccccaacggc     1200

cggaaggtgg acctgcagct gggcaacctg ggctacctgc agtccttcaa ctacaggatc     1260

gacaccaccg ccaccagctg ccagctgtac tacaacctgc ctgctgctaa cgtgtccgtg     1320

agcaggttca accctagcac ctggaaccgc agattcggct tcaccgagca gtccgtgttc     1380

aagccccagc ctgccggcgt gttcaccgac cacgacgtgg tgtacgccca gcactgcttc     1440

aaggccccca ccaacttctg cccttgcaag ctggacggca gcctgtgcgt gggatccgga     1500

agcggcatcg acgccggcta caagaacacc ggcatcggaa cctgccctgc tggaaccaac     1560

tacctgacct gccacaacgc tgctcagtgc gactgcctgt gcacccccga ccctatcacc     1620

tccaaggcca ccggccccta caagtgccct cagaccaagt acctggtggg aatcggagag     1680

cactgctccg gcctggccat caagagcgac cactgcggag gaaacccttg cagctgccag     1740

cctcaggcct tcctgggatg gtccgtggac agctgcctgc agggcgaccg ctgcaacatc     1800

ttcgccaact tcatcctgca cgacgtgaac tccggcacca cctgcagcac cgacctgcag     1860

aagagcaaca ccgacatcat cctgggcgtg tgcgtgaact acgacctgta cggcatcacc     1920

ggccagggca tcttcgtgga ggtgaacgcc acctactaca actcctggca gaacctgctg     1980

tacgacagca acggcaacct gtacggcttc cgcgactacc tgaccaaccg gaccttcatg     2040

atcaggtcct gctacagcgg cagagtgagc gccgctttcc acgccaacag ctccgagccc     2100

gccctgctgt tccggaacat caagtgcaac tacgtgttca acaacaccct gtccaggcag     2160

ctgcagccta tcaactactt cgacagctac ctgggctgcg tggtgaacgc cgacaactcc     2220

accagctccg tggtgcagac ctgcgacctg accgtgggat ccggatactg cgtggactac     2280

agcaccaagg ccgcctccgc cgccagcatc accaccggct acagattcac caacttcgag     2340

cccttcaccg tgaactccgt gaacgacagc ctggagcccg tgggaggcct gtacgagatc     2400

cagatcccta gcgagttcac catcggcaac atggaggagt tcatccagac cagctcccct     2460

aaggtgacca tcgactgctc cgccttcgtg tgcggcgact acgctgcttg caagtcccag     2520

ctggtggagt acggcagctt ctgcgacaac atcaacgcca tcctgaccga ggtgaacgag     2580

ctgctggaca ccacccagct gcaggtggcc aactccctga tgaacggcgt gaccctgagc     2640

accaagctga aggacggcgt gaacttcaac gtggacgata tcaacttctc ccccgtgctg     2700

ggatgcctgg gaagcgactg caacaaggtg agctcccggt ccgccatcga ggacctgctg     2760

ttctccaagg tgaagctgag cgacgtgggc ttcgtggagg cctacaacaa ctgcacaggc     2820

ggagctgaga tcagggacct gatctgcgtg cagagctaca acggcatcaa ggtgctgccc     2880

cctctgctgt ccgagaacca gatcagcggc tacaccctgg ctgccacctc cgccagcctg     2940

ttccctcctt ggagcgccgc cgccggggtg cccttctacc tgaacgtgca gtaccgcatc     3000

aacggcatcg gcgtgaccat ggacgtgctg agccagaacc agaagctgat cgccaacgcc     3060

ttcaacaacg ctctgggagc tatccaggag ggattcgacg ctaccaacag cgccctggtg     3120

aagatccagg ctgtggtgaa cgctaacgct gaggccctga acaacctgct gcagcagctg     3180

agcaaccgct tcggcgccat cagctccagc ctgcaggaga tcctgtccag actggacgcc     3240

ctggaggctc aggctcagat cgaccgcctg atcaacggca gactgaccgc cctgaacgcc     3300

tacgtgagcc agcagctgtc cgacagcacc ctggtgaagt tctccgccgc ccaggccatg     3360

gagaaggtga acgagtgcgt gaagagccag tccagccgca tcaacttctg cggcaacggc     3420

aaccacatca tctccctggt gcagaacgcc ccctacggcc tgtacttcat ccacttcagc     3480

tacgtgccca ccaagtacgt gaccgccaag gtgtcccctg gcctgtgcat cgccggggac     3540

cggggcatcg cccctaagag cggctacttc gtgaacgtga acaacacctg gatgttcacc     3600

ggctccggct actactaccc cgagcctatc accggcaaca acgtggtggt catgagcacc     3660

tgcgccgtga actacaccaa ggcccccgac gtgatgctga acatctccac ccccaacctg     3720

cctgacttca aggaggagct ggaccagtgg ttcaagaacc agacctccgt ggcccctgac     3780

ctgagcctgg actacatcaa cgtgaccttc ctggacctgc aggacgagat gaacaggctg     3840

caggaggcca tcaaggtgct gaaccagtcc tacatcaacc tgaaggacat cggcacctac     3900

gagtactacg tgaagtggcc cagctccatc gccagcttct tcttcatcat cggcctgatc     3960

atcggcctgt tcctggtgct gcgggtgggc atctacctgt gcatcaagct gaagcacacc     4020

aagaagcggc agatctacac cgacatcgag atgaacaggc tgggcaagta a              4071


<210>  24
<211>  3351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SADS-CoV Spike protein with VSV G protein TMD-CTD region

<400>  24
atgaagctgt ttaccgtgtt taccctgctc gcctcaatca gagtgctcta tggctgtgaa       60

tccgtggact ttaacctgtt taataccatc ttctccaccc acaggggcct gtccaacacc      120

accagcgtga tcaccggcgc ctaccccagc accaacaagt ccgactggag ctgcaacacc      180

aggaccggac acctgtccgg aagcggattc ggaatcggcc tgtacgtgca gacccccaga      240

gagcagtacc agtacgacgg atccggagct ggaggataca ccatcgccgt gagcccaatc      300

cacgtgacca acctgacctg ggagctgtgg atccacagaa agtggggcgt gaactccgtg      360

gtgaccgtga ggctgtgcag atggtggcag ttcatgagct tcaactccac cagccacgct      420

gctgacgctg gacccaccaa cgccttcgag tgcctgatca acggcagcta cccaacccac      480

cgcaacaccg gctacatgtt cggcgtgacc tggtacaacg acctggtgcg gatcgtgttc      540

cccccaaccg tgctggagat gcagctggac ggcctgcagt gggagagggt gcagttcaac      600

tccccagtga acgctggaca cgctacccgg ttcaacgtgg tgaaggacat ctccaccgtg      660

ctggtggaga caaactccgg cggcagcgtg ttcaggtaca gctactgcgc cgacggcttc      720

gtgaacggcc tgcagtgcaa gctgagactg ttcgacatcc ccccaggcgt gtactccaac      780

agcgaggtgg agtacccaac cgccctgtac accgtggtgc acaacatgtc cgcctgcccc      840

gagaggccag acagctactg cggctccaac agctgcccct tcaagagagc cgtgttcagc      900

aactgcatcg tgaactacac cacctgggtg aacccagacc agcgcgactt ccagcacctg      960

atcctgccca acggcaagtt caacccattc accgagtgca acggcctgaa caggatcgtg     1020

gacggatgcg tgccaggatt cgtgctgagg gtgggaaggg gcaaggccgt gaaccggacc     1080

atcgtgaccc cctacctgaa gccatacgag tgcttcggct ggtcctggaa cgacaaccag     1140

gacagcatct acgactggtg gatcgccgac ttcgtgtcca ccggagcctt cgtgtgcgag     1200

agcaacccag aggctccaaa gaccggcgtg tgcgtgacct acaccgtgga gaaggtgacc     1260

ttccagggcg tgctgtacga gtccaacttc accttcgccc agtactacaa cctgctgtac     1320

gtgggcagcc agctgagata cgtgagaatc ctgggcaagg tgtacgaggt gtccagctgt     1380

ttcgaggcca gctacgacgt gctgtaccgc aacaaccagt ccttcggcct gctgtaccgg     1440

agcttcgact gcaaccagct gcacatcaag tccgccagat tcgtggacag gctgctgcca     1500

agccacaacg gaaccgccac cgtgctgggc tgcctgttca acgcctccta cgcccccaac     1560

gacaccatgg tgaactgcac caacccactg ggcgacggat tctgcgctga cctgctggga     1620

aacgtggccg tgcgcaggat gaccttcgag aagcacgaca ccacctacgt ggcccccgtg     1680

accaacgaga ggtacaccga gatgccactg gaccaccagc tgatcctgac cgagcagttc     1740

ctgcagacca ccatgccaaa gttctccgtg agctgcgaga catacatctg cgacgtgagc     1800

aaggcctgca agaacctgct gttcaggtac ggcggcttct gccagaaggt ggaggccgac     1860

atcagaggag ctggcatcct gctggacggc gacgtgtcca gcctgtactc caccatcgct     1920

gctaagacct ccagcgtggt gccaaccacc gacaggttca acgtgagcca gttcttcctg     1980

ccaaagaccc agtccagcgc caacaagtac gagtccagaa gcgccatcga ggacctgctg     2040

ttctccaaga tcgagacaac cggccccggc ttctacggcg actactacaa ctgcaagaag     2100

aacgccatcc aggacctgac ctgcgcccag taccacaacg gcatcctggt catcccccca     2160

atcatggacg ccgagacact gggaatgtac ggaggaatcg ctgccgcctc cgtgaccctg     2220

ggaatcttcg gaggacaggc tggaatggct acctggagcg tggccatggc cggccggctg     2280

aacgctctgg gagtggtgca gaacgctctg gtggacgacg tgaacaagct ggccaacggc     2340

ttcaaccagc tgaccgcctc cgtgagcaag ctggccctga ccacctccag cgccctgcag     2400

gctatccagg ctgtggtgaa ccagaacgct gctcaggtgg agtccctggt gagcggcatc     2460

accgagaact tcggcgccat ctccaccaac ttcaaagtga tcagccagcg cctggacaag     2520

ctggaggctg acgtgcagat ggaccgcctg atcaacggcc ggatgaacgt gctgcagctg     2580

ttcgtgacca actacaagct gaagatcgcc gagctgagga acacccacag atacgtgcag     2640

tccctgatca acgagtgcgt gtacgctcag agcctgagga acggattctg cggacagggc     2700

ctgcacgtgc tgtccctgat gcagaacgcc cccagcggca tcatgttctt ccactacagc     2760

ctgatcccca acaacaccat caccgtgaag accaccccag gcctgtgcga gtccgacgag     2820

ctgggaagca agtgcatcgt ggctaaggac ggcgtgctgg tgtccgccaa cctgagctac     2880

tggcagtggt ccccccgcaa cctgtacaag ccagagaacc tgaccttcgc caacgtgatc     2940

gccgtgagca ggggagctaa ctacaccacc ctgaacaaga ccttcgacat ccccgagctg     3000

aactccacct tcccaatcga ggaggagttc cgcgagtact tccagaacat gtccagcgag     3060

ctgcaggtgc tgaagaacct gaccgccgac atgtccaagc tgaacatcag cgccgagatc     3120

cagctgatca acgagatcgc ccacaacgtg tccaacatga gggtggaggt ggagaagttc     3180

cagagatacg tgaactacgt gaagtcctcc atcgcctcct tcttcttcat catcggcctg     3240

atcatcggcc tgttcctggt gctgcgcgtg ggcatctacc tgtgcatcaa gctgaagcac     3300

accaagaagc gccagatcta caccgacatc gagatgaacc gcctgggcaa g              3351


