﻿               SEQUENCE LISTING

<110> Ishchuk Olena
      Petranovic Nielsen Dina

<120> A GENETICALLY MODIFIED YEAST CELL

<130> P42000589SE00

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 1220
<212> DNA
<213> yeast


<220> 
<223> Yeast cell ROX1 gene sequence deleted from genome

<400> 1
gaaaatacta atacttcttc acacaaaaga acgcagttag acaatcaaca atgaatccta      60

aatcctctac acctaagatt ccaagaccca agaacgcatt tattctgttc agacagcact     120

accacaggat cttaatagac gaatggaccg ctcaaggtgt ggaaataccc cataattcaa     180

acatttctaa aattattggt acgaagtgga agggcttaca accggaagat aaggcacact     240

gggaaaatct agcggagaag gagaaactag aacatgaaag gaagtatcct gaatacaaat     300

acaagccggt aagaaagtct aagaagaagc aactactttt gaaggaaatc gagcaacagc     360

agcagcaaca acagaaagaa cagcagcagc agaaacagtc acaaccgcaa ttacaacagc     420

cctttaacaa caatatagtt cttatgaaaa gagcacattc tctttcacca tcttcctcgg     480

tgtcaagctc gaacagctat cagttccaat tgaacaatga tcttaagagg ttgcctattc     540

cttctgttaa tacttctaac tatatggtct ccagatcttt aagtggacta cctttgacgc     600

atgataagac ggcaagagac ctaccacagc tgtcatctca actaaattct attccatatt     660

actcagctcc acacgaccct tcaacgagac atcattacct caacgtcgct caagctcaac     720

caagggctaa ctcgacccct caattgccct ttatttcatc cattatcaac aacagcagtc     780

aaacaccggt aactacaact accacatcca caacaactgc gacatcttct cctgggaaat     840

tctcctcttc tccgaactcc tctgtactgg agaacaacag attaaacagt atcaacaatt     900

caaatcaata tttacctccc cctctattac cttctctgca agattttcaa ctggatcagt     960

accagcagct aaagcagatg ggaccaactt atattgtcaa accactgtct cacaccagga    1020

acaatctatt gtccacaact acccctacgc atcatcacat tcctcatata ccaaaccaaa    1080

acattcctct acatcaaatt ataaactcaa gcaacactga ggtcaccgct aaaactagcc    1140

tagtttctcc gaaatgattt tttttttcca tttcttcttt ccgttatatt atattatact    1200

atattccctt taactaaaaa                                                1220


<210> 2
<211> 4670
<212> DNA
<213> Yeast


<220> 
<223> Yeast cell VPS10 gene sequence deleted from genome

<400> 2
tttaactaat gccgaagaat tcacccccaa agtgacaaag actatcgcgc aagattcatt       60

tgatatatta agctttgatg attccaacac tttaattaga aaacaagaca cctccgttac      120

tataagcttt gacgatggtg aaacatggga aaaagttgaa ggcattgaag gcgaaatcac      180

ttggatatac atcgacccct tcaatagaca tgacagagcc gttgcaacgg caatgaatgg      240

gtcgtacctt tatataacca atgatcaagg taagtcatgg gagcgtataa cgctacctga      300

ctccggagaa agtatttcac ctcgtgaatg ctatatagaa acccatccct tgaataagaa      360

ctattttctt gcaaagtgca actattgtga gaaaacagaa gtaaacaatg acaatgaaga      420

gaattcgggg gacgaagagg gacaatttga aatatttaat attacacgtt gcacagacaa      480

ggtttttgca agtaatgatg gtggaaaatc cttttctgag atcaagtctt ctctagaaag      540

gaacgaaaat agtcctatca gcatttctga ttgtggcttt gccaagacca gcaaagattc      600

tgaccttgaa agtagtgata cctcaataat ctgtcttttt caaaatatgc agcttattat      660

ggatgagttt agttctcctt acaccgaaag taaattggtc ctaactacgg actggggcaa      720

atcactaaaa gaatttgacc aatttaaaga taaggtcgtc aatggttaca ggatattgaa      780

atctcacatg gttgtcttaa cccagggcga cagatataat gatatgtctt ccatggatgt      840

gtgggtatca aatgatctgt caaactttaa aatggcttac atgcctactc agttaaggca      900

ttctatgcaa ggagaaatat atgaggacgc tatgggaaga attatcttgc ccatgagtag      960

ggaaagaagt gatcaagagg aggataaggg catcgtgtct gaaattttaa tttccgactc     1020

acaagggtta aaattttccc ccatcccatg gaccgcaaat gaggtgtttg gttatattaa     1080

tttttatcaa cctacttact tgaaaggaac gatgattgcc tcactttacc ctctatctag     1140

gcgtcgtaac cgtaaaggaa aagccaaagg agtaaagagt aagggggtaa ccaaaatatc     1200

tgttgataat ggcctcacat ggacaatgtt aaaagttgtt gatccagata acgcagactc     1260

attcgactgt gatattactg attttgagaa ttgttcgctt caaaatatgt tttacacacg     1320

ggagggttcc actccaaccg ccggaattct aatgacaaca ggtattgttg gcgatggtag     1380

tgtcttcgac tggggagatc aaagaacctt tatttctagg gatggtggct taacatggaa     1440

actcgccttt gattttcctt gtttatacgc tgttggtgat tacggaaatg ttattgtggc     1500

tataccgtat aatgcggatg aagacgacga tcctcaatcc gaattttatt actctttaga     1560

ccaaggtaaa acttggaccg aatatcagct agaaactact atctacccaa atgaagtaat     1620

gaatacaacg cccgacggat ctggagctaa atttattcta aatgggttta ctttggcgca     1680

tatggatggt acaacgaatt tcatctatgc aattgatttt tcaacagcct ttaatgataa     1740

gacatgcgaa gaaaatgatt tcgaggattg gaatttagct gaggggaagt gtgtcaatgg     1800

agtcaagtac aagatcagaa gaagaaaaca ggacgctcag tgcttggtga agaaagtttt     1860

tgaagactta caattatttg agactgcttg tgacaagtgt accgaggctg attacgaatg     1920

cgcgtttgaa tttgttaggg acgcgaccgg gaaatgcgta ccagactaca acctaatcgt     1980

tctctctgac gtatgtgata agacaaagaa aaaaactgtg cctgtaaaac cattgcaact     2040

agttaaaggt gataaatgta aaaaaccaat gacagtcaaa tcagtggata tttcgtgtga     2100

gggagttcca aagaagggaa cgaatgataa agaaatagtg gttacagaaa acaaatttga     2160

tttcaagatt caattctatc aatactttga cacagtcacc gacgaatccc tcctcatgat     2220

caattcaaga ggagaagctt atatatctca tgatggtgga caaacaataa aaaggttcga     2280

cagtaatggt gaaacaatta ttgaagttgt gtttaatcca tactacaatt cttcagctta     2340

tctgtttggt tccaaaggta gcattttctc tacccatgat aggggatact cttttatgac     2400

tgctaaattg cccgaggcta ggcagttagg tatgccatta gactttaacg ctaaggcaca     2460

ggatacattt atctattatg gtggtaagaa ttgtgagtca atcttaagtc cggaatgtca     2520

tgcggtagca tacctgacca atgatggggg cgaaacgttt acggaaatgc ttgataatgc     2580

aattcattgt gagtttgcgg gctcactttt caaatatccg tcaaatgagg atatggttat     2640

gtgtcaagtg aaggaaaagt cttcgcagac aagaagctta gtttcttcta ctgatttttt     2700

ccaggatgat aaaaataccg tctttgaaaa tattatcggc tacttatcca ctggtggcta     2760

tatcatcgtt gctgttcctc atgagaacaa cgaattgaga gcatacgtaa ctatcgatgg     2820

tactgagttt gccgaggcaa aattcccata tgatgaagat gttgggaagc aagaggcatt     2880

cactatatta gagtctgaga aaggatcgat attcttacat ttagcaacaa acttagtacc     2940

aggacgcgat tttggcaatc ttttgaaatc caactcaaat ggtacttctt ttgtcacgtt     3000

ggagcatgcc gttaatagaa acacattcgg ctatgttgac tttgaaaaaa ttcaaggtct     3060

cgaaggcatt attctcacca acatcgtttc aaatagtgac aaggtcgccg agaataaaga     3120

agacaaacaa ttgaagacga agatcacctt taatgaaggt tcagattgga actttttgaa     3180

acctccgaag agggattcag aaggaaaaaa gttttcttgc agctccaaat cactggatga     3240

gtgttcattg cacttacatg gctatactga acgtaaggat attagagata catattcttc     3300

cggttctgca ttaggaatga tgttcggcgt tggcaacgtt ggtcctaacc ttttaccata     3360

taaagaatgt tccaccttct tcaccaccga tggtggcgaa acgtgggctg aagttaagaa     3420

gactcctcac caatgggaat acggtgacca cggtgggatt ttagttttag ttcctgaaaa     3480

ctcagaaact gattctattt cctattctac cgattttggt aaaacatgga aagattataa     3540

attctgcgct gataaggttt tagtaaagga tataaccact gttcccaggg attctgcttt     3600

gagatttttg ctgtttggag aggcagcaga tattggaggc agctcattta gaacgtacac     3660

aattgatttt agaaacatct tcgaaagaca atgtgatttc gacatcactg gtaaggaaag     3720

cgcagattat aaatactctc ctctgggttc caaaagcaat tgcctatttg gtcaccaaac     3780

cgagttttta cgtaaaaccg atgaaaattg ttttattggg aatattccac tttctgaatt     3840

ttcaagaaat atcaaaaact gttcttgtac aagacaagat ttcgagtgtg attacaactt     3900

ttacaaagct aacgatggta cttgtaaatt agtcaaagga ctaagcccag caaatgctgc     3960

agacgtttgt aaaaaagagc cagatttaat cgaatatttt gaatcgtcag gctacagaaa     4020

gatccctcta tcaacctgtg agggtggcct gaaattggat gctccctcat caccacatgc     4080

ttgcccagga aaagaaaaag aattcaagga aaagtactca gtaagtgccg gtccctttgc     4140

atttattttc atttcaattc ttttaataat tttctttgcc gcatggtttg tatatgacag     4200

aggtatcaga agaaatgggg gatttgcaag gtttggagaa attaggctag gtgacgatgg     4260

tttaatagaa aacaataata ctgacagagt tgtcaataac attgtgaaat caggatttta     4320

cgttttctca aatatcgggt ctcttttaca gcacacaaaa actaatatag cgcatgctat     4380

ctccaaaatt agaggaaggt ttggaaacag aacaggtcca agctactcat ccctgatcca     4440

tgatcaattt ttggatgaag cagatgacct gcttgctggc cacgatgaag acgccaatga     4500

cttatccagt ttcatggatc agggtagtaa ttttgaaatc gaagaagatg atgttccaac     4560

acttgaagaa gagcatacat catatacaga tcaacctacg accaccgatg ttccagatac     4620

attaccagaa ggaaatgagg aaaacatcga caggcctgat tctacagcgc                4670


<210> 3
<211> 1095
<212> DNA
<213> Yeast


<220> 
<223> Yeast cell HMX1 gene sequence deleted from genome

<400> 3
aatataacac agcatatata cacacacaca cataaaataa ccgcaaaaat ggaggacagt      60

agcaatacaa tcataccctc acccactgac gtgggggcgc tagcaaacag aatcaacttt     120

caaaccagag atgcccacaa taaaatcaat accttcatgg gcataaagat ggccatcgcc     180

atgagacatg gctttatata cagacagggt attctggcgt actattatgt gttcgatgcc     240

atcgagcaag agatagatcg cctactgaat gaccccgtaa cggaggagga gctgcaaact     300

tcgaccattc tgaagcagtt ttggctcgaa gattttagaa gatctacgca gatctataag     360

gacctgaagc tgctatactc aaacacgttt aaaagcacag aatcattaaa cgaattcctg     420

gctacgttcc agaagccacc gctactacag cagtttatca ataacatcca cgaaaacata     480

cacaaggagc catgcaccat tctttcttac tgtcacgttc tgtacttggc gcttttcgcc     540

ggcggcaagc taatacgatc gaatttgtac agaagactgg ggctcttccc caacttcgag     600

aagctatcac agaaggaact ggtcaaaaag ggcacaaact tcttcacctt cagcgatctg     660

ggtcccactg aagaaacacg cttgaaatgg gaatacaaga agaactatga gctggccacc     720

aggacggaat tgaccgaagc acaaaagttg cagatcatta gcgtcgcaga aggcattttt     780

gattggaact tcaacatcgt tgcagaaatt ggagagttga atcgtcgcga gttaatgggc     840

aagttcagct tcaagtgtat tacgtacttg tacgaagaat ggatgttcaa caaggattct     900

gctactagaa gagcactcca cacggtcatg ctgctggtgc tttctattat cgcgatctgg     960

gttctttact tcttggtaaa gagttttctt agcatagtat aacgtataaa aaaaaaagtc    1020

taaatacaaa cataatatat acatgaaata atatcaaaat atgaagaata gggaaggaaa    1080

aacagcagag aaaaa                                                     1095


<210> 4
<211> 1181
<212> DNA
<213> Yeast


<220> 
<223> Yeast cell PEP4 gene sequence deleted from genome

<400> 4
gccaaccaag ttgctgcaaa agtccacaag gctaaaattt ataaacacga gttgtccgat      60

gagatgaaag aagtcacttt cgagcaacat ttagctcatt taggccaaaa gtacttgact     120

caatttgaga aagctaaccc cgaagttgtt ttttctaggg agcatccttt cttcactgaa     180

ggtggtcacg atgttccatt gacaaattac ttgaacgcac aatattacac tgacattact     240

ttgggtactc cacctcaaaa cttcaaggtt attttggata ctggttcttc aaacctttgg     300

gttccaagta acgaatgtgg ttccttggct tgtttcctac attctaaata cgatcatgaa     360

gcttcatcaa gctacaaagc taatggtact gaatttgcca ttcaatatgg tactggttct     420

ttggaaggtt acatttctca agacactttg tccatcgggg atttgaccat tccaaaacaa     480

gacttcgctg aggctaccag cgagccgggc ttaacatttg catttggcaa gttcgatggt     540

attttgggtt tgggttacga taccatttct gttgataagg tggtccctcc attttacaac     600

gccattcaac aagatttgtt ggacgaaaag agatttgcct tttatttggg agacacttca     660

aaggatactg aaaatggcgg tgaagccacc tttggtggta ttgacgagtc taagttcaag     720

ggcgatatca cttggttacc tgttcgtcgt aaggcttact gggaagtcaa gtttgaaggt     780

atcggtttag gcgacgagta cgccgaattg gagagccatg gtgccgccat cgatactggt     840

acttctttga ttaccttgcc atcaggatta gctgaaatga ttaatgctga aattggggcc     900

aagaagggtt ggaccggtca atatactcta gactgtaaca ccagagacaa tctacctgat     960

ctaattttca acttcaatgg ctacaacttc actattgggc catacgatta cacgcttgaa    1020

gtttcaggct cctgtatctc tgcaattaca ccaatggatt tcccagaacc tgttggccca    1080

ctggccatcg ttggtgatgc cttcttgcgt aaatactatt ctatttacga tttgggcaac    1140

aatgcggttg gtttggccaa agcaatttga gctaaacttt t                        1181


<210> 5
<211> 309
<212> DNA
<213> Yeast


<220> 
<223> Yeast cell AHSP gene sequence

<400> 5
atggctttat taaaagccaa taaggattta atttcagcag gtttgaaaga atttagtgtt     60

cttctaaatc aacaagtttt taatgatcct ttggtctctg aagaagatat ggttactgtt    120

gttgaagatt ggatgaattt ttatattaat tattataaac aacaagttac tggtgaacct    180

caagaaagag ataaagctct acaagaacta aggcaagaac taaatactct agctaatcca    240

tttttggcta aatatagaga ttttctaaaa tctcatgaat tgccatctca tccaccacca    300

tcttcttag                                                            309


<210> 6
<211> 102
<212> PRT
<213> Yeast


<220> 
<223> Yeast cell AHSP polypeptide sequence

<400> 6
Met Ala Leu Leu Lys Ala Asn Lys Asp Leu Ile Ser Ala Gly Leu Lys 
1               5                   10                  15      
Glu Phe Ser Val Leu Leu Asn Gln Gln Val Phe Asn Asp Pro Leu Val 
            20                  25                  30          
Ser Glu Glu Asp Met Val Thr Val Val Glu Asp Trp Met Asn Phe Tyr 
        35                  40                  45              
Ile Asn Tyr Tyr Lys Gln Gln Val Thr Gly Glu Pro Gln Glu Arg Asp 
    50                  55                  60                  
Lys Ala Leu Gln Glu Leu Arg Gln Glu Leu Asn Thr Leu Ala Asn Pro 
65                  70                  75                  80  
Phe Leu Ala Lys Tyr Arg Asp Phe Leu Lys Ser His Glu Leu Pro Ser 
                85                  90                  95      
His Pro Pro Pro Ser Ser 
            100         

<210> 7
<211> 984
<212> DNA
<213> Yeast


<220> 
<223> Yeast cell HEM3 gene

<400> 7
atgggccctg aaactctaca tattggtggg agaaaatcga aattggcggt aatacaatcc      60

aaccatgttt taaaactgat cgaagaaaag tatccggact acgactgcaa ggttttcact     120

ttgcaaactc ttggtgacca gattcaattc aaacctttgt actcatttgg cggtaaagct     180

ttatggacaa aggagttgga agaccatctt taccatgacg atccctcaaa gaagcttgac     240

ttgatcgttc attctctgaa ggacatgccc actttactac cagagggttt cgagctgggg     300

ggtatcacta agcgggtcga tccaacagat tgtcttgtca tgccctttta ctctgcttat     360

aagtctctgg atgaccttcc agacgggggg attgtgggaa cctcatccgt gagaagatct     420

gctcagctaa aaagaaaata cccacatttg aaatttgaaa gtgtcagagg aaatatacaa     480

actagattac aaaaactaga cgacccaaaa tctccgtacc aatgcatcat cttggcgtct     540

gctgggttga tgcgtatggg gttggaaaac agaattacgc agcgattcca ttcggataca     600

atgtaccatg cagttggaca aggcgccctg ggtatagaaa ttagaaaggg tgacaccaag     660

atgatgaaga ttcttgacga aatttgcgat ctaaatgcaa ctatatgttg cctttcggag     720

cgtgctttga tgagaacttt agaggggggt tgttccgttc ctattggtgt ggaatctaaa     780

tacaatgaag agactaaaaa attactatta aaggccattg tagttgacgt tgaaggcaca     840

gaagcagtag aagacgaaat tgaaatgcta atagaaaatg ttaaagaaga ttccatggcg     900

tgtggtaaga tactagctga aagaatgatt gccgatggcg caaagaaaat tctggatgaa     960

attaatttag acagaatcaa atga                                            984


<210> 8
<211> 327
<212> PRT
<213> Yeast


<220> 
<223> Yeast cell Hem3 polypeptide sequence

<400> 8
Met Gly Pro Glu Thr Leu His Ile Gly Gly Arg Lys Ser Lys Leu Ala 
1               5                   10                  15      
Val Ile Gln Ser Asn His Val Leu Lys Leu Ile Glu Glu Lys Tyr Pro 
            20                  25                  30          
Asp Tyr Asp Cys Lys Val Phe Thr Leu Gln Thr Leu Gly Asp Gln Ile 
        35                  40                  45              
Gln Phe Lys Pro Leu Tyr Ser Phe Gly Gly Lys Ala Leu Trp Thr Lys 
    50                  55                  60                  
Glu Leu Glu Asp His Leu Tyr His Asp Asp Pro Ser Lys Lys Leu Asp 
65                  70                  75                  80  
Leu Ile Val His Ser Leu Lys Asp Met Pro Thr Leu Leu Pro Glu Gly 
                85                  90                  95      
Phe Glu Leu Gly Gly Ile Thr Lys Arg Val Asp Pro Thr Asp Cys Leu 
            100                 105                 110         
Val Met Pro Phe Tyr Ser Ala Tyr Lys Ser Leu Asp Asp Leu Pro Asp 
        115                 120                 125             
Gly Gly Ile Val Gly Thr Ser Ser Val Arg Arg Ser Ala Gln Leu Lys 
    130                 135                 140                 
Arg Lys Tyr Pro His Leu Lys Phe Glu Ser Val Arg Gly Asn Ile Gln 
145                 150                 155                 160 
Thr Arg Leu Gln Lys Leu Asp Asp Pro Lys Ser Pro Tyr Gln Cys Ile 
                165                 170                 175     
Ile Leu Ala Ser Ala Gly Leu Met Arg Met Gly Leu Glu Asn Arg Ile 
            180                 185                 190         
Thr Gln Arg Phe His Ser Asp Thr Met Tyr His Ala Val Gly Gln Gly 
        195                 200                 205             
Ala Leu Gly Ile Glu Ile Arg Lys Gly Asp Thr Lys Met Met Lys Ile 
    210                 215                 220                 
Leu Asp Glu Ile Cys Asp Leu Asn Ala Thr Ile Cys Cys Leu Ser Glu 
225                 230                 235                 240 
Arg Ala Leu Met Arg Thr Leu Glu Gly Gly Cys Ser Val Pro Ile Gly 
                245                 250                 255     
Val Glu Ser Lys Tyr Asn Glu Glu Thr Lys Lys Leu Leu Leu Lys Ala 
            260                 265                 270         
Ile Val Val Asp Val Glu Gly Thr Glu Ala Val Glu Asp Glu Ile Glu 
        275                 280                 285             
Met Leu Ile Glu Asn Val Lys Glu Asp Ser Met Ala Cys Gly Lys Ile 
    290                 295                 300                 
Leu Ala Glu Arg Met Ile Ala Asp Gly Ala Lys Lys Ile Leu Asp Glu 
305                 310                 315                 320 
Ile Asn Leu Asp Arg Ile Lys 
                325         

<210> 9
<211> 354
<212> DNA
<213> Homo sapiens


<220> 
<223> Sequence encoding human hemoglobin subunit alpha

<400> 9
atggtcttgt ctcctgccga taaaactaat gtaaaagctg cctggggtaa agtaggtgcc      60

cacgccggtg aatatcacgg ttcagcacaa gtcaagggtc atggtaaaaa ggtagccgac     120

gctttgacca atgcagttgc ccacgtcgat gacatgccaa acgctttgtc cgcattaagt     180

gatttgcatg ctcacaagtt gagagtagac cctgttaact tcaagttgtt gtcccattgt     240

ttgttagtta ccttagctgc acacttgcca gccgaattca ctcctgctgt ccatgcttct     300

ttagacaagt ttttagcatc cgtttccacc gttttgacct ccaagtatag ataa           354


<210> 10
<211> 444
<212> DNA
<213> Homo sapiens


<220> 
<223> Sequence encoding human hemoglobin subunit beta

<400> 10
atggtacatt tgactccaga agaaaagtcc gccgtaacag cattatgggg taaagtaaac      60

gtagacgaag ttggtggtga agccttaggt agattgttag ttgtctatcc atggacccaa     120

agatttttcg aatcttttgg tgacttgtct actccagacg ccgtaatggg taatcctaaa     180

gttaaggctc atggtaaaaa ggttttgggt gctttttccg atggtttggc acacttagac     240

aacttgaaag gtactttcgc aacattgtct gaattgcatt gtgataagtt acacgttgat     300

cctgaaaact tcagattgtt gggtaacgta ttagtttgcg tcttggctca tcactttggt     360

aaagaattca ctccacctgt tcaagcagcc tatcaaaagg ttgtcgcagg tgttgctaac     420

gcattggcac ataagtatca ctga                                            444


<210> 11
<211> 117
<212> PRT
<213> Homo sapiens


<220> 
<223> Human hemoglobin subunit alpha

<400> 11
Met Val Leu Ser Pro Ala Asp Lys Thr Asn Val Lys Ala Ala Trp Gly 
1               5                   10                  15      
Lys Val Gly Ala His Ala Gly Glu Tyr His Gly Ser Ala Gln Val Lys 
            20                  25                  30          
Gly His Gly Lys Lys Val Ala Asp Ala Leu Thr Asn Ala Val Ala His 
        35                  40                  45              
Val Asp Asp Met Pro Asn Ala Leu Ser Ala Leu Ser Asp Leu His Ala 
    50                  55                  60                  
His Lys Leu Arg Val Asp Pro Val Asn Phe Lys Leu Leu Ser His Cys 
65                  70                  75                  80  
Leu Leu Val Thr Leu Ala Ala His Leu Pro Ala Glu Phe Thr Pro Ala 
                85                  90                  95      
Val His Ala Ser Leu Asp Lys Phe Leu Ala Ser Val Ser Thr Val Leu 
            100                 105                 110         
Thr Ser Lys Tyr Arg 
        115         

<210> 12
<211> 147
<212> PRT
<213> Homo sapiens


<220> 
<223> Human hemoglobin subunit beta

<400> 12
Met Val His Leu Thr Pro Glu Glu Lys Ser Ala Val Thr Ala Leu Trp 
1               5                   10                  15      
Gly Lys Val Asn Val Asp Glu Val Gly Gly Glu Ala Leu Gly Arg Leu 
            20                  25                  30          
Leu Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Glu Ser Phe Gly Asp 
        35                  40                  45              
Leu Ser Thr Pro Asp Ala Val Met Gly Asn Pro Lys Val Lys Ala His 
    50                  55                  60                  
Gly Lys Lys Val Leu Gly Ala Phe Ser Asp Gly Leu Ala His Leu Asp 
65                  70                  75                  80  
Asn Leu Lys Gly Thr Phe Ala Thr Leu Ser Glu Leu His Cys Asp Lys 
                85                  90                  95      
Leu His Val Asp Pro Glu Asn Phe Arg Leu Leu Gly Asn Val Leu Val 
            100                 105                 110         
Cys Val Leu Ala His His Phe Gly Lys Glu Phe Thr Pro Pro Val Gln 
        115                 120                 125             
Ala Ala Tyr Gln Lys Val Val Ala Gly Val Ala Asn Ala Leu Ala His 
    130                 135                 140                 
Lys Tyr His 
145         

<210> 13
<211> 1173
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence encoding hemoglobin secreted from modified yeast cell,
      ∆rox1∆vps10∆hmx1∆pep4/HEM3+alpha leader-Hbfusion

<400> 13
atgagatttc catctatttt tactgctgtt ttgtttgctg cttcttctgc tttggctgct      60

ccagttaata ctactactga agatgaaact gctcaaattc cagctgaagc tgttattggt     120

tattctgatt tggagggtga ctttgatgtt gctgttttgc cattttctaa ctctactaac     180

aacggtttgc tattcatcaa cactactatc gcttctatcg ctgctaaaga agaaggtgtt     240

tctttggata aaagagaaga aggtgaacca aaagtcttat ctcctgccga taaaacaaat     300

gtaaaagctg cctggggtaa agtaggtgct catgccggtg aatacggtgc tgaagcctta     360

gaaagaatgt ttttgtcttt cccaactaca aagacctact tccctcattt cgatttgtct     420

cacggttcag cacaagtcaa gggtcatggt aaaaaggtag ccgacgcttt gacaaatgca     480

gtcgcccacg tagatgacat gccaaacgcc ttgtccgctt taagtgattt gcatgctcac     540

aaattaagag ttgaccctgt caacttcaag ttgttgtccc attgtttgtt ggttactttg     600

gctgcacact tgccagccga attcactcct gcagttcatg cctccttaga taaattcttg     660

gcttccgtaa gtacagtttt gaccagtaag tatagaggtg gttctggtgg ttcaggtggt     720

tccggtggta gtggtcattt tactgaagaa gataaagcta ccatcacttc attatggggt     780

aaagtaaacg ttgaagacgc aggtggtgaa accttgggta gattgttagt tgtctaccca     840

tggactcaaa gatttttcga ttctttcggt aatttgtctt ctgcttcagc aattatgggt     900

aaccctaaag ttaaggctca tggtaaaaag gtcttaactt ctttgggtga cgctattaaa     960

cacttagatg acttgaaggg tacatttgcc caattatcag aattgcattg cgataaattg    1020

cacgttgacc cagaaaattt caagttgttg ggtaacgtct tagtaacagt tttggcaatc    1080

catttcggta aagaattcac ccctgaagtt caagcaagtt ggcaaaagat ggtcacaggt    1140

gtcgcctccg cattgagttc cagatatcat tga                                 1173


<210> 14
<211> 390
<212> PRT
<213> Artificial Sequence


<220> 
<223> Polypeptide sequence of hemoglobin secreted from modified yeast
      cell, ∆rox1∆vps10∆hmx1∆pep4/HEM3+alpha leader-Hbfusion

<400> 14
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  
Ser Leu Asp Lys Arg Glu Glu Gly Glu Pro Lys Val Leu Ser Pro Ala 
                85                  90                  95      
Asp Lys Thr Asn Val Lys Ala Ala Trp Gly Lys Val Gly Ala His Ala 
            100                 105                 110         
Gly Glu Tyr Gly Ala Glu Ala Leu Glu Arg Met Phe Leu Ser Phe Pro 
        115                 120                 125             
Thr Thr Lys Thr Tyr Phe Pro His Phe Asp Leu Ser His Gly Ser Ala 
    130                 135                 140                 
Gln Val Lys Gly His Gly Lys Lys Val Ala Asp Ala Leu Thr Asn Ala 
145                 150                 155                 160 
Val Ala His Val Asp Asp Met Pro Asn Ala Leu Ser Ala Leu Ser Asp 
                165                 170                 175     
Leu His Ala His Lys Leu Arg Val Asp Pro Val Asn Phe Lys Leu Leu 
            180                 185                 190         
Ser His Cys Leu Leu Val Thr Leu Ala Ala His Leu Pro Ala Glu Phe 
        195                 200                 205             
Thr Pro Ala Val His Ala Ser Leu Asp Lys Phe Leu Ala Ser Val Ser 
    210                 215                 220                 
Thr Val Leu Thr Ser Lys Tyr Arg Gly Gly Ser Gly Gly Ser Gly Gly 
225                 230                 235                 240 
Ser Gly Gly Ser Gly His Phe Thr Glu Glu Asp Lys Ala Thr Ile Thr 
                245                 250                 255     
Ser Leu Trp Gly Lys Val Asn Val Glu Asp Ala Gly Gly Glu Thr Leu 
            260                 265                 270         
Gly Arg Leu Leu Val Val Tyr Pro Trp Thr Gln Arg Phe Phe Asp Ser 
        275                 280                 285             
Phe Gly Asn Leu Ser Ser Ala Ser Ala Ile Met Gly Asn Pro Lys Val 
    290                 295                 300                 
Lys Ala His Gly Lys Lys Val Leu Thr Ser Leu Gly Asp Ala Ile Lys 
305                 310                 315                 320 
His Leu Asp Asp Leu Lys Gly Thr Phe Ala Gln Leu Ser Glu Leu His 
                325                 330                 335     
Cys Asp Lys Leu His Val Asp Pro Glu Asn Phe Lys Leu Leu Gly Asn 
            340                 345                 350         
Val Leu Val Thr Val Leu Ala Ile His Phe Gly Lys Glu Phe Thr Pro 
        355                 360                 365             
Glu Val Gln Ala Ser Trp Gln Lys Met Val Thr Gly Val Ala Ser Ala 
    370                 375                 380                 
Leu Ser Ser Arg Tyr His 
385                 390 

