                         SEQUENCE LISTING

<110>  Gyroscope Therapeutics Limited
 
<120>  Methods of Producing Recombinant Complement Proteins, Vectors and
        Therapeutic Uses Thereof

<130>  P123973PCT

<140>  PCT/GB2021/053009
<141>  2021-11-19

<150>  GB 2018320.8
<151>  2020-11-20

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  583
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Lys Leu Leu His Val Phe Leu Leu Phe Leu Cys Phe His Leu Arg 
1               5                   10                  15      


Phe Cys Lys Val Thr Tyr Thr Ser Gln Glu Asp Leu Val Glu Lys Lys 
            20                  25                  30          


Cys Leu Ala Lys Lys Tyr Thr His Leu Ser Cys Asp Lys Val Phe Cys 
        35                  40                  45              


Gln Pro Trp Gln Arg Cys Ile Glu Gly Thr Cys Val Cys Lys Leu Pro 
    50                  55                  60                  


Tyr Gln Cys Pro Lys Asn Gly Thr Ala Val Cys Ala Thr Asn Arg Arg 
65                  70                  75                  80  


Ser Phe Pro Thr Tyr Cys Gln Gln Lys Ser Leu Glu Cys Leu His Pro 
                85                  90                  95      


Gly Thr Lys Phe Leu Asn Asn Gly Thr Cys Thr Ala Glu Gly Lys Phe 
            100                 105                 110         


Ser Val Ser Leu Lys His Gly Asn Thr Asp Ser Glu Gly Ile Val Glu 
        115                 120                 125             


Val Lys Leu Val Asp Gln Asp Lys Thr Met Phe Ile Cys Lys Ser Ser 
    130                 135                 140                 


Trp Ser Met Arg Glu Ala Asn Val Ala Cys Leu Asp Leu Gly Phe Gln 
145                 150                 155                 160 


Gln Gly Ala Asp Thr Gln Arg Arg Phe Lys Leu Ser Asp Leu Ser Ile 
                165                 170                 175     


Asn Ser Thr Glu Cys Leu His Val His Cys Arg Gly Leu Glu Thr Ser 
            180                 185                 190         


Leu Ala Glu Cys Thr Phe Thr Lys Arg Arg Thr Met Gly Tyr Gln Asp 
        195                 200                 205             


Phe Ala Asp Val Val Cys Tyr Thr Gln Lys Ala Asp Ser Pro Met Asp 
    210                 215                 220                 


Asp Phe Phe Gln Cys Val Asn Gly Lys Tyr Ile Ser Gln Met Lys Ala 
225                 230                 235                 240 


Cys Asp Gly Ile Asn Asp Cys Gly Asp Gln Ser Asp Glu Leu Cys Cys 
                245                 250                 255     


Lys Ala Cys Gln Gly Lys Gly Phe His Cys Lys Ser Gly Val Cys Ile 
            260                 265                 270         


Pro Ser Gln Tyr Gln Cys Asn Gly Glu Val Asp Cys Ile Thr Gly Glu 
        275                 280                 285             


Asp Glu Val Gly Cys Ala Gly Phe Ala Ser Val Thr Gln Glu Glu Thr 
    290                 295                 300                 


Glu Ile Leu Thr Ala Asp Met Asp Ala Glu Arg Arg Arg Ile Lys Ser 
305                 310                 315                 320 


Leu Leu Pro Lys Leu Ser Cys Gly Val Lys Asn Arg Met His Ile Arg 
                325                 330                 335     


Arg Lys Arg Ile Val Gly Gly Lys Arg Ala Gln Leu Gly Asp Leu Pro 
            340                 345                 350         


Trp Gln Val Ala Ile Lys Asp Ala Ser Gly Ile Thr Cys Gly Gly Ile 
        355                 360                 365             


Tyr Ile Gly Gly Cys Trp Ile Leu Thr Ala Ala His Cys Leu Arg Ala 
    370                 375                 380                 


Ser Lys Thr His Arg Tyr Gln Ile Trp Thr Thr Val Val Asp Trp Ile 
385                 390                 395                 400 


His Pro Asp Leu Lys Arg Ile Val Ile Glu Tyr Val Asp Arg Ile Ile 
                405                 410                 415     


Phe His Glu Asn Tyr Asn Ala Gly Thr Tyr Gln Asn Asp Ile Ala Leu 
            420                 425                 430         


Ile Glu Met Lys Lys Asp Gly Asn Lys Lys Asp Cys Glu Leu Pro Arg 
        435                 440                 445             


Ser Ile Pro Ala Cys Val Pro Trp Ser Pro Tyr Leu Phe Gln Pro Asn 
    450                 455                 460                 


Asp Thr Cys Ile Val Ser Gly Trp Gly Arg Glu Lys Asp Asn Glu Arg 
465                 470                 475                 480 


Val Phe Ser Leu Gln Trp Gly Glu Val Lys Leu Ile Ser Asn Cys Ser 
                485                 490                 495     


Lys Phe Tyr Gly Asn Arg Phe Tyr Glu Lys Glu Met Glu Cys Ala Gly 
            500                 505                 510         


Thr Tyr Asp Gly Ser Ile Asp Ala Cys Lys Gly Asp Ser Gly Gly Pro 
        515                 520                 525             


Leu Val Cys Met Asp Ala Asn Asn Val Thr Tyr Val Trp Gly Val Val 
    530                 535                 540                 


Ser Trp Gly Glu Asn Cys Gly Lys Pro Glu Phe Pro Gly Val Tyr Thr 
545                 550                 555                 560 


Lys Val Ala Asn Tyr Phe Asp Trp Ile Ser Tyr His Val Gly Arg Pro 
                565                 570                 575     


Phe Ile Ser Gln Tyr Asn Val 
            580             


<210>  2
<211>  317
<212>  PRT
<213>  Homo sapiens

<400>  2

Lys Val Thr Tyr Thr Ser Gln Glu Asp Leu Val Glu Lys Lys Cys Leu 
1               5                   10                  15      


Ala Lys Lys Tyr Thr His Leu Ser Cys Asp Lys Val Phe Cys Gln Pro 
            20                  25                  30          


Trp Gln Arg Cys Ile Glu Gly Thr Cys Val Cys Lys Leu Pro Tyr Gln 
        35                  40                  45              


Cys Pro Lys Asn Gly Thr Ala Val Cys Ala Thr Asn Arg Arg Ser Phe 
    50                  55                  60                  


Pro Thr Tyr Cys Gln Gln Lys Ser Leu Glu Cys Leu His Pro Gly Thr 
65                  70                  75                  80  


Lys Phe Leu Asn Asn Gly Thr Cys Thr Ala Glu Gly Lys Phe Ser Val 
                85                  90                  95      


Ser Leu Lys His Gly Asn Thr Asp Ser Glu Gly Ile Val Glu Val Lys 
            100                 105                 110         


Leu Val Asp Gln Asp Lys Thr Met Phe Ile Cys Lys Ser Ser Trp Ser 
        115                 120                 125             


Met Arg Glu Ala Asn Val Ala Cys Leu Asp Leu Gly Phe Gln Gln Gly 
    130                 135                 140                 


Ala Asp Thr Gln Arg Arg Phe Lys Leu Ser Asp Leu Ser Ile Asn Ser 
145                 150                 155                 160 


Thr Glu Cys Leu His Val His Cys Arg Gly Leu Glu Thr Ser Leu Ala 
                165                 170                 175     


Glu Cys Thr Phe Thr Lys Arg Arg Thr Met Gly Tyr Gln Asp Phe Ala 
            180                 185                 190         


Asp Val Val Cys Tyr Thr Gln Lys Ala Asp Ser Pro Met Asp Asp Phe 
        195                 200                 205             


Phe Gln Cys Val Asn Gly Lys Tyr Ile Ser Gln Met Lys Ala Cys Asp 
    210                 215                 220                 


Gly Ile Asn Asp Cys Gly Asp Gln Ser Asp Glu Leu Cys Cys Lys Ala 
225                 230                 235                 240 


Cys Gln Gly Lys Gly Phe His Cys Lys Ser Gly Val Cys Ile Pro Ser 
                245                 250                 255     


Gln Tyr Gln Cys Asn Gly Glu Val Asp Cys Ile Thr Gly Glu Asp Glu 
            260                 265                 270         


Val Gly Cys Ala Gly Phe Ala Ser Val Ala Gln Glu Glu Thr Glu Ile 
        275                 280                 285             


Leu Thr Ala Asp Met Asp Ala Glu Arg Arg Arg Ile Lys Ser Leu Leu 
    290                 295                 300                 


Pro Lys Leu Ser Cys Gly Val Lys Asn Arg Met His Ile 
305                 310                 315         


<210>  3
<211>  244
<212>  PRT
<213>  Homo sapiens

<400>  3

Ile Val Gly Gly Lys Arg Ala Gln Leu Gly Asp Leu Pro Trp Gln Val 
1               5                   10                  15      


Ala Ile Lys Asp Ala Ser Gly Ile Thr Cys Gly Gly Ile Tyr Ile Gly 
            20                  25                  30          


Gly Cys Trp Ile Leu Thr Ala Ala His Cys Leu Arg Ala Ser Lys Thr 
        35                  40                  45              


His Arg Tyr Gln Ile Trp Thr Thr Val Val Asp Trp Ile His Pro Asp 
    50                  55                  60                  


Leu Lys Arg Ile Val Ile Glu Tyr Val Asp Arg Ile Ile Phe His Glu 
65                  70                  75                  80  


Asn Tyr Asn Ala Gly Thr Tyr Gln Asn Asp Ile Ala Leu Ile Glu Met 
                85                  90                  95      


Lys Lys Asp Gly Asn Lys Lys Asp Cys Glu Leu Pro Arg Ser Ile Pro 
            100                 105                 110         


Ala Cys Val Pro Trp Ser Pro Tyr Leu Phe Gln Pro Asn Asp Thr Cys 
        115                 120                 125             


Ile Val Ser Gly Trp Gly Arg Glu Lys Asp Asn Glu Arg Val Phe Ser 
    130                 135                 140                 


Leu Gln Trp Gly Glu Val Lys Leu Ile Ser Asn Cys Ser Lys Phe Tyr 
145                 150                 155                 160 


Gly Asn Arg Phe Tyr Glu Lys Glu Met Glu Cys Ala Gly Thr Tyr Asp 
                165                 170                 175     


Gly Ser Ile Asp Ala Cys Lys Gly Asp Ser Gly Gly Pro Leu Val Cys 
            180                 185                 190         


Met Asp Ala Asn Asn Val Thr Tyr Val Trp Gly Val Val Ser Trp Gly 
        195                 200                 205             


Glu Asn Cys Gly Lys Pro Glu Phe Pro Gly Val Tyr Thr Lys Val Ala 
    210                 215                 220                 


Asn Tyr Phe Asp Trp Ile Ser Tyr His Val Gly Arg Pro Phe Ile Ser 
225                 230                 235                 240 


Gln Tyr Asn Val 
                


<210>  4
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  4

Arg Arg Lys Arg 
1               


<210>  5
<211>  794
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Glu Leu Arg Pro Trp Leu Leu Trp Val Val Ala Ala Thr Gly Thr 
1               5                   10                  15      


Leu Val Leu Leu Ala Ala Asp Ala Gln Gly Gln Lys Val Phe Thr Asn 
            20                  25                  30          


Thr Trp Ala Val Arg Ile Pro Gly Gly Pro Ala Val Ala Asn Ser Val 
        35                  40                  45              


Ala Arg Lys His Gly Phe Leu Asn Leu Gly Gln Ile Phe Gly Asp Tyr 
    50                  55                  60                  


Tyr His Phe Trp His Arg Gly Val Thr Lys Arg Ser Leu Ser Pro His 
65                  70                  75                  80  


Arg Pro Arg His Ser Arg Leu Gln Arg Glu Pro Gln Val Gln Trp Leu 
                85                  90                  95      


Glu Gln Gln Val Ala Lys Arg Arg Thr Lys Arg Asp Val Tyr Gln Glu 
            100                 105                 110         


Pro Thr Asp Pro Lys Phe Pro Gln Gln Trp Tyr Leu Ser Gly Val Thr 
        115                 120                 125             


Gln Arg Asp Leu Asn Val Lys Ala Ala Trp Ala Gln Gly Tyr Thr Gly 
    130                 135                 140                 


His Gly Ile Val Val Ser Ile Leu Asp Asp Gly Ile Glu Lys Asn His 
145                 150                 155                 160 


Pro Asp Leu Ala Gly Asn Tyr Asp Pro Gly Ala Ser Phe Asp Val Asn 
                165                 170                 175     


Asp Gln Asp Pro Asp Pro Gln Pro Arg Tyr Thr Gln Met Asn Asp Asn 
            180                 185                 190         


Arg His Gly Thr Arg Cys Ala Gly Glu Val Ala Ala Val Ala Asn Asn 
        195                 200                 205             


Gly Val Cys Gly Val Gly Val Ala Tyr Asn Ala Arg Ile Gly Gly Val 
    210                 215                 220                 


Arg Met Leu Asp Gly Glu Val Thr Asp Ala Val Glu Ala Arg Ser Leu 
225                 230                 235                 240 


Gly Leu Asn Pro Asn His Ile His Ile Tyr Ser Ala Ser Trp Gly Pro 
                245                 250                 255     


Glu Asp Asp Gly Lys Thr Val Asp Gly Pro Ala Arg Leu Ala Glu Glu 
            260                 265                 270         


Ala Phe Phe Arg Gly Val Ser Gln Gly Arg Gly Gly Leu Gly Ser Ile 
        275                 280                 285             


Phe Val Trp Ala Ser Gly Asn Gly Gly Arg Glu His Asp Ser Cys Asn 
    290                 295                 300                 


Cys Asp Gly Tyr Thr Asn Ser Ile Tyr Thr Leu Ser Ile Ser Ser Ala 
305                 310                 315                 320 


Thr Gln Phe Gly Asn Val Pro Trp Tyr Ser Glu Ala Cys Ser Ser Thr 
                325                 330                 335     


Leu Ala Thr Thr Tyr Ser Ser Gly Asn Gln Asn Glu Lys Gln Ile Val 
            340                 345                 350         


Thr Thr Asp Leu Arg Gln Lys Cys Thr Glu Ser His Thr Gly Thr Ser 
        355                 360                 365             


Ala Ser Ala Pro Leu Ala Ala Gly Ile Ile Ala Leu Thr Leu Glu Ala 
    370                 375                 380                 


Asn Lys Asn Leu Thr Trp Arg Asp Met Gln His Leu Val Val Gln Thr 
385                 390                 395                 400 


Ser Lys Pro Ala His Leu Asn Ala Asn Asp Trp Ala Thr Asn Gly Val 
                405                 410                 415     


Gly Arg Lys Val Ser His Ser Tyr Gly Tyr Gly Leu Leu Asp Ala Gly 
            420                 425                 430         


Ala Met Val Ala Leu Ala Gln Asn Trp Thr Thr Val Ala Pro Gln Arg 
        435                 440                 445             


Lys Cys Ile Ile Asp Ile Leu Thr Glu Pro Lys Asp Ile Gly Lys Arg 
    450                 455                 460                 


Leu Glu Val Arg Lys Thr Val Thr Ala Cys Leu Gly Glu Pro Asn His 
465                 470                 475                 480 


Ile Thr Arg Leu Glu His Ala Gln Ala Arg Leu Thr Leu Ser Tyr Asn 
                485                 490                 495     


Arg Arg Gly Asp Leu Ala Ile His Leu Val Ser Pro Met Gly Thr Arg 
            500                 505                 510         


Ser Thr Leu Leu Ala Ala Arg Pro His Asp Tyr Ser Ala Asp Gly Phe 
        515                 520                 525             


Asn Asp Trp Ala Phe Met Thr Thr His Ser Trp Asp Glu Asp Pro Ser 
    530                 535                 540                 


Gly Glu Trp Val Leu Glu Ile Glu Asn Thr Ser Glu Ala Asn Asn Tyr 
545                 550                 555                 560 


Gly Thr Leu Thr Lys Phe Thr Leu Val Leu Tyr Gly Thr Ala Pro Glu 
                565                 570                 575     


Gly Leu Pro Val Pro Pro Glu Ser Ser Gly Cys Lys Thr Leu Thr Ser 
            580                 585                 590         


Ser Gln Ala Cys Val Val Cys Glu Glu Gly Phe Ser Leu His Gln Lys 
        595                 600                 605             


Ser Cys Val Gln His Cys Pro Pro Gly Phe Ala Pro Gln Val Leu Asp 
    610                 615                 620                 


Thr His Tyr Ser Thr Glu Asn Asp Val Glu Thr Ile Arg Ala Ser Val 
625                 630                 635                 640 


Cys Ala Pro Cys His Ala Ser Cys Ala Thr Cys Gln Gly Pro Ala Leu 
                645                 650                 655     


Thr Asp Cys Leu Ser Cys Pro Ser His Ala Ser Leu Asp Pro Val Glu 
            660                 665                 670         


Gln Thr Cys Ser Arg Gln Ser Gln Ser Ser Arg Glu Ser Pro Pro Gln 
        675                 680                 685             


Gln Gln Pro Pro Arg Leu Pro Pro Glu Val Glu Ala Gly Gln Arg Leu 
    690                 695                 700                 


Arg Ala Gly Leu Leu Pro Ser His Leu Pro Glu Val Val Ala Gly Leu 
705                 710                 715                 720 


Ser Cys Ala Phe Ile Val Leu Val Phe Val Thr Val Phe Leu Val Leu 
                725                 730                 735     


Gln Leu Arg Ser Gly Phe Ser Phe Arg Gly Val Lys Val Tyr Thr Met 
            740                 745                 750         


Asp Arg Gly Leu Ile Ser Tyr Lys Gly Leu Pro Pro Glu Ala Trp Gln 
        755                 760                 765             


Glu Glu Cys Pro Ser Asp Ser Glu Glu Asp Glu Gly Arg Gly Glu Arg 
    770                 775                 780                 


Thr Ala Phe Ile Lys Asp Gln Ser Ala Leu 
785                 790                 


<210>  6
<211>  1179
<212>  DNA
<213>  Homo sapiens

<400>  6
ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg       60

ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt      120

gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca      180

gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc      240

gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt      300

acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg      360

gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg      420

cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct      480

ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg      540

caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc      600

gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga      660

gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct      720

ggtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca      780

gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg      840

acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg      900

tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat      960

tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg     1020

gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa     1080

ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca     1140

gtggttcaaa gtttttttct tccatttcag gtgtcgtga                            1179


<210>  7
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak Sequence

<400>  7
gccacc                                                                   6


<210>  8
<211>  2385
<212>  DNA
<213>  Homo sapiens

<400>  8
atggagctga ggccctggtt gctatgggtg gtagcagcaa caggaacctt ggtcctgcta       60

gcagctgatg ctcagggcca gaaggtcttc accaacacgt gggctgtgcg catccctgga      120

ggcccagcgg tggccaacag tgtggcacgg aagcatgggt tcctcaacct gggccagatc      180

ttcggggact attaccactt ctggcatcga ggagtgacga agcggtccct gtcgcctcac      240

cgcccgcggc acagccggct gcagagggag cctcaagtac agtggctgga acagcaggtg      300

gcaaagcgac ggactaaacg ggacgtgtac caggagccca cagaccccaa gtttcctcag      360

cagtggtacc tgtctggtgt cactcagcgg gacctgaatg tgaaggcggc ctgggcgcag      420

ggctacacag ggcacggcat tgtggtctcc attctggacg atggcatcga gaagaaccac      480

ccggacttgg caggcaatta tgatcctggg gccagttttg atgtcaatga ccaggaccct      540

gacccccagc ctcggtacac acagatgaat gacaacaggc acggcacacg gtgtgcgggg      600

gaagtggctg cggtggccaa caacggtgtc tgtggtgtag gtgtggccta caacgcccgc      660

attggagggg tgcgcatgct ggatggcgag gtgacagatg cagtggaggc acgctcgctg      720

ggcctgaacc ccaaccacat ccacatctac agtgccagct ggggccccga ggatgacggc      780

aagacagtgg atgggccagc ccgcctcgcc gaggaggcct tcttccgtgg ggttagccag      840

ggccgagggg ggctgggctc catctttgtc tgggcctcgg ggaacggggg ccgggaacat      900

gacagctgca actgcgacgg ctacaccaac agtatctaca cgctgtccat cagcagcgcc      960

acgcagtttg gcaacgtgcc gtggtacagc gaggcctgct cgtccacact ggccacgacc     1020

tacagcagtg gcaaccagaa tgagaagcag atcgtgacga ctgacttgcg gcagaagtgc     1080

acggagtctc acacgggcac ctcagcctct gcccccttag cagccggcat cattgctctc     1140

accctggagg ccaataagaa cctcacatgg cgggacatgc aacacctggt ggtacagacc     1200

tcgaagccag cccacctcaa tgccaacgac tgggccacca atggtgtggg ccggaaagtg     1260

agccactcat atggctacgg gcttttggac gcaggcgcca tggtggccct ggcccagaat     1320

tggaccacag tggcccccca gcggaagtgc atcatcgaca tcctcaccga gcccaaagac     1380

atcgggaaac ggctcgaggt gcggaagacc gtgaccgcgt gcctgggcga gcccaaccac     1440

atcactcggc tggagcacgc tcaggcgcgg ctcaccctgt cctataatcg ccgtggcgac     1500

ctggccatcc acctggtcag ccccatgggc acccgctcca ccctgctggc agccaggcca     1560

catgactact ccgcagatgg gtttaatgac tgggccttca tgacaactca ttcctgggat     1620

gaggatccct ctggcgagtg ggtcctagag attgaaaaca ccagcgaagc caacaactat     1680

gggacgctga ccaagttcac cctcgtactc tatggcaccg cccctgaggg gctgcccgta     1740

cctccagaaa gcagtggctg caagaccctc acgtccagtc aggcctgtgt ggtgtgcgag     1800

gaaggcttct ccctgcacca gaagagctgt gtccagcact gccctccagg cttcgccccc     1860

caagtcctcg atacgcacta tagcaccgag aatgacgtgg agaccatccg ggccagcgtc     1920

tgcgccccct gccacgcctc atgtgccaca tgccaggggc cggccctgac agactgcctc     1980

agctgcccca gccacgcctc cttggaccct gtggagcaga cttgctcccg gcaaagccag     2040

agcagccgag agtccccgcc acagcagcag ccacctcggc tgcccccgga ggtggaggcg     2100

gggcaacggc tgcgggcagg gctgctgccc tcacacctgc ctgaggtggt ggccggcctc     2160

agctgcgcct tcatcgtgct ggtcttcgtc actgtcttcc tggtcctgca gctgcgctct     2220

ggctttagtt ttcggggggt gaaggtgtac accatggacc gtggcctcat ctcctacaag     2280

gggctgcccc ctgaagcctg gcaggaggag tgcccgtctg actcagaaga ggacgagggc     2340

cggggcgaga ggaccgcctt tatcaaagac cagagcgccc tctga                     2385


<210>  9
<211>  588
<212>  DNA
<213>  Encephalomyocarditis virus

<400>  9
gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt       60

gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc      120

ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag      180

gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac      240

aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc      300

tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc      360

acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca      420

aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt      480

gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg      540

gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaacc                   588


<210>  10
<211>  1752
<212>  DNA
<213>  Homo sapiens

<400>  10
atgaagcttc ttcatgtttt cctgttattt ctgtgcttcc acttaaggtt ttgcaaggtc       60

acttatacat ctcaagagga tctggtggag aaaaagtgct tagcaaaaaa atatactcac      120

ctctcctgcg ataaagtctt ctgccagcca tggcagagat gcattgaggg cacctgtgtt      180

tgtaaactac cgtatcagtg cccaaagaat ggcactgcag tgtgtgcaac taacaggaga      240

agcttcccaa catactgtca acaaaagagt ttggaatgtc ttcatccagg gacaaagttt      300

ttaaataacg gaacatgcac agccgaagga aagtttagtg tttccttgaa gcatggaaat      360

acagattcag agggaatagt tgaagtaaaa cttgtggacc aagataagac aatgttcata      420

tgcaaaagca gctggagcat gagggaagcc aacgtggcct gccttgacct tgggtttcaa      480

caaggtgctg atactcaaag aaggtttaag ttgtctgatc tctctataaa ttccactgaa      540

tgtctacatg tgcattgccg aggattagag accagtttgg ctgaatgtac ttttactaag      600

agaagaacta tgggttacca ggatttcgct gatgtggttt gttatacaca gaaagcagat      660

tctccaatgg atgacttctt tcagtgtgtg aatgggaaat acatttctca gatgaaagcc      720

tgtgatggta tcaatgattg tggagaccaa agtgatgaac tgtgttgtaa agcatgccaa      780

ggcaaaggct tccattgcaa atcgggtgtt tgcattccaa gccagtatca atgcaatggt      840

gaggtggact gcattacagg ggaagatgaa gttggctgtg caggctttgc atctgtggct      900

caagaagaaa cagaaatttt gactgctgac atggatgcag aaagaagacg gataaaatca      960

ttattaccta aactatcttg tggagttaaa aacagaatgc acattcgaag gaaacgaatt     1020

gtgggaggaa agcgagcaca actgggagac ctcccatggc aggtggcaat taaggatgcc     1080

agtggaatca cctgtggggg aatttatatt ggtggctgtt ggattctgac tgctgcacat     1140

tgtctcagag ccagtaaaac tcatcgttac caaatatgga caacagtagt agactggata     1200

caccccgacc ttaaacgtat agtaattgaa tacgtggata gaattatttt ccatgaaaac     1260

tacaatgcag gcacttacca aaatgacatc gctttgattg aaatgaaaaa agacggaaac     1320

aaaaaagatt gtgagctgcc tcgttccatc cctgcctgtg tcccctggtc tccttaccta     1380

ttccaaccta atgatacatg catcgtttct ggctggggac gagaaaaaga taacgaaaga     1440

gtcttttcac ttcagtgggg tgaagttaaa ctaataagca actgctctaa gttttacgga     1500

aatcgtttct atgaaaaaga aatggaatgt gcaggtacat atgatggttc catcgatgcc     1560

tgtaaagggg actctggagg ccccttagtc tgtatggatg ccaacaatgt gacttatgtc     1620

tggggtgttg tgagttgggg ggaaaactgt ggaaaaccag agttcccagg tgtttacacc     1680

aaagtggcca attattttga ctggattagc taccatgtag gaaggccttt tatttctcag     1740

tacaatgtat aa                                                         1752


<210>  11
<211>  222
<212>  DNA
<213>  Simian virus 40

<400>  11
cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa       60

aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca      120

ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt      180

gggaggtttt ttaaagcaag taaaacctct acaaatgtgg ta                         222


<210>  12
<211>  588
<212>  DNA
<213>  Human cytomegalovirus

<400>  12
gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata       60

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc      120

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag      180

ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac      240

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg      300

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg      360

tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat      420

agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt      480

tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc      540

aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctc                   588


<210>  13
<211>  1026
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hygromycin resistance marker

<400>  13
atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac       60

agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat      120

gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat      180

cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt      240

ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg      300

caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat      360

gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga      420

atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat      480

cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag      540

ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc      600

tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg      660

atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct      720

tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg      780

cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac      840

ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga      900

gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc      960

tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag     1020

gaatag                                                                1026


<210>  14
<211>  225
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bovine Growth Hormone late polyadenylation signal

<400>  14
ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc       60

tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc      120

tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt      180

gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgg                      225


<210>  15
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC origin of replication

<400>  15
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg       60

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      120

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctct cttcgggaag      180

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      240

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      300

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      360

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      420

taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac      480

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      540

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa                  589


<210>  16
<211>  861
<212>  DNA
<213>  Escherichia coli

<400>  16
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat       60

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc      120

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa      180

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca      240

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa      300

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt      360

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc      420

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact      480

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc      540

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg      600

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct      660

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc      720

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag      780

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac      840

acggaaatgt tgaatactca t                                                861


<210>  17
<211>  585
<212>  DNA
<213>  Human cytomegalovirus

<400>  17
ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc       60

ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca      120

ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta      180

tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta      240

tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat      300

cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga      360

ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca      420

aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg      480

taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc      540

ctggagacgc catccacgct gttttgacct ccatagaaga caccg                      585


<210>  18
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAG promoter

<400>  18
ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga       60

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat      120

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc      180

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct      240

attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc      300

ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg      360

gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg      420

cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg      480

aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg      540

cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga      600

ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat      660

tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg      720

ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg      780

ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag      840

agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct      900

ggttattgtg ctgtctcatc attttggcaa agaattggat cc                         942


<210>  19
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE

<400>  19
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt      120

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg      180

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact      240

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct      300

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg      360

ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc      420

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc      480

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt      540

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgc                  589


<210>  20
<211>  245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE3

<400>  20
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt      120

atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc      180

atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc      240

gtggt                                                                  245


<210>  21
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bovine Growth Hormone poly-A (bGH poly-A) signal

<400>  21
cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga       60

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt      120

gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg      180

attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgg                   228


<210>  22
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Bovine Growth Hormone poly-A (bGH poly-A) signal

<400>  22
tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc       60

cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga      120

aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga      180

cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat      240

ggcttctgag gcggaaagaa ccagctgggg                                       270


