                         SEQUENCE LISTING

<110>  shen, zhejin
 
<120>  蝮蛇蛋白水解酶在治疗老年痴呆上的应用  Use of 
       Agkistrodon halys protease for the treatment of Alzheimer's 
       Disease

<130>  PROTEASE-AD

<140>  PCT-AD-001
<141>  2020-11-09

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  222
<212>  PRT
<213>  Gloydius ussuriensis  白眉蝮蛇

<400>  1

Val Ile Gly Gly Val Glu Cys Asn Ile Asn Glu His Arg Phe Leu Val 
1               5                   10                  15      


Ala Leu Tyr Thr Ser Arg Ser Arg Arg Phe Tyr Cys Gly Gly Thr Leu 
            20                  25                  30          


Ile Asn Gln Glu Trp Val Leu Thr Ala Ala His Cys Asp Arg Lys Asn 
        35                  40                  45              


Ile Arg Ile Lys Leu Gly Met His Ser Glu Lys Val Pro Asn Glu Asp 
    50                  55                  60                  


Ala Gln Thr Arg Val Pro Lys Glu Lys Phe Phe Cys Leu Ser Ser Lys 
65                  70                  75                  80  


Thr Tyr Thr Lys Trp Asp Lys Asp Ile Met Leu Met Arg Leu Lys Arg 
                85                  90                  95      


Pro Val Asn Asn Ser Thr His Ile Ala Pro Val Ser Leu Pro Ser Asn 
            100                 105                 110         


Pro Pro Ser Val Gly Ser Val Cys Arg Val Met Gly Trp Gly Thr Ile 
        115                 120                 125             


Thr Ser Ser Gln Glu Thr His Pro Asp Val Pro His Cys Ala Asn Ile 
    130                 135                 140                 


Asn Ile Leu Asp Tyr Glu Val Cys Arg Ala Ala Tyr Pro Glu Leu Pro 
145                 150                 155                 160 


Val Thr Arg Arg Thr Leu Cys Ala Gly Ile Leu Glu Gly Gly Lys Asp 
                165                 170                 175     


Ser Cys Asn Gly Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly Gln Phe 
            180                 185                 190         


Gln Gly Ile Ala Tyr Trp Gly Ala Asp Thr Cys Ala Gln Pro Arg Glu 
        195                 200                 205             


Pro Gly Leu Tyr Thr Lys Val Phe Asp Tyr Thr Asp Trp Ile 
    210                 215                 220         


<210>  2
<211>  219
<212>  PRT
<213>  Gloydius ussuriensis白眉蝮蛇

<400>  2

Val Ile Gly Gly Val Glu Cys Asn Ile Asn Glu His Gly Phe Leu Ala 
1               5                   10                  15      


Leu Leu Tyr Ser Arg Arg Phe Gln Cys Gly Gly Thr Leu Ile Asn Glu 
            20                  25                  30          


Glu Trp Val Leu Thr Ala Ala His Cys Asp Met Arg Asn Met Tyr Ile 
        35                  40                  45              


Tyr Leu Gly Val His Asn Val Ser Val Gln Tyr Asp Asp Glu Gln Arg 
    50                  55                  60                  


Arg Tyr Pro Lys Lys Lys Tyr Phe Cys Leu Ser Ser Arg Asn Tyr Asn 
65                  70                  75                  80  


Gln Trp Asp Lys Asp Ile Met Leu Ile Arg Leu Asn Arg Pro Val Arg 
                85                  90                  95      


Asn Ser Ala His Ile Ala Pro Leu Ser Leu Pro Ser Asn Pro Pro Ser 
            100                 105                 110         


Val Gly Ser Val Cys Arg Val Met Gly Trp Gly Thr Ile Thr Ser Pro 
        115                 120                 125             


Gln Glu Thr Leu Pro Asp Val Pro His Cys Ala Asn Ile Asn Ile Leu 
    130                 135                 140                 


Asp Tyr Glu Val Cys Arg Ala Ala Tyr Pro Glu Leu Pro Val Thr Arg 
145                 150                 155                 160 


Arg Thr Leu Cys Ala Gly Ile Leu Glu Gly Gly Lys Asp Ser Cys Asn 
                165                 170                 175     


Gly Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly Gln Phe Gln Gly Ile 
            180                 185                 190         


Ala Tyr Trp Gly Ala Asp Thr Cys Ala Gln Pro Arg Glu Pro Gly Leu 
        195                 200                 205             


Tyr Thr Lys Val Phe Asp Tyr Thr Asp Trp Ile 
    210                 215                 


<210>  3
<211>  222
<212>  PRT
<213>  Gloydius ussuriensis白眉蝮蛇

<400>  3

Ile Ile Gly Gly Asp Glu Cys Asn Ile Asn Glu His Arg Phe Leu Val 
1               5                   10                  15      


Ala Leu Tyr Thr Ser Arg Ser Arg Arg Phe Tyr Cys Gly Gly Thr Leu 
            20                  25                  30          


Ile Asn Gln Glu Trp Val Leu Thr Ala Ala His Cys Asp Arg Lys Asn 
        35                  40                  45              


Ile Arg Ile Lys Leu Gly Met His Ser Glu Lys Val Pro Asn Glu Asp 
    50                  55                  60                  


Ala Gln Thr Arg Val Pro Lys Glu Lys Phe Phe Cys Leu Ser Ser Lys 
65                  70                  75                  80  


Thr Tyr Thr Lys Trp Asp Lys Asp Ile Met Leu Met Arg Leu Lys Arg 
                85                  90                  95      


Pro Val Asn Asn Ser Thr His Ile Ala Pro Val Ser Leu Pro Ser Asn 
            100                 105                 110         


Pro Pro Ser Val Gly Ser Val Cys Arg Val Met Gly Trp Gly Thr Ile 
        115                 120                 125             


Thr Ser Pro Gln Glu Thr Tyr Pro Asp Val Pro His Cys Ala Asn Ile 
    130                 135                 140                 


Asn Ile Leu Asp Tyr Glu Val Cys Gln Ala Ala His Gly Gly Leu Pro 
145                 150                 155                 160 


Ala Thr Ser Arg Thr Leu Cys Ala Gly Ile Leu Lys Gly Gly Lys Asp 
                165                 170                 175     


Ser Cys Lys Gly Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly Gln Phe 
            180                 185                 190         


Gln Gly Ile Ala Ser Trp Gly Ala His Pro Cys Gly Gln Ser Leu Lys 
        195                 200                 205             


Pro Gly Val Tyr Thr Lys Val Phe Asp Tyr Thr Glu Trp Ile 
    210                 215                 220         


<210>  4
<211>  224
<212>  PRT
<213>  Gloydius halys江浙蝮蛇

<400>  4

Ile Ile Gly Gly Asp Glu Cys Asn Ile Asn Glu His Arg Phe Leu Val 
1               5                   10                  15      


Ala Leu Tyr Thr Ser Arg Ser Arg Thr Leu Phe Cys Gly Gly Thr Leu 
            20                  25                  30          


Ile Asn Gln Glu Trp Val Leu Thr Ala Ala His Cys Asp Arg Lys Asn 
        35                  40                  45              


Phe Arg Ile Lys Leu Gly Met His Ser Lys Lys Val Pro Asn Glu Asp 
    50                  55                  60                  


Glu Gln Thr Arg Val Pro Lys Glu Lys Phe Phe Cys Leu Ser Ser Lys 
65                  70                  75                  80  


Asn Tyr Thr Leu Trp Asp Lys Asp Ile Met Leu Ile Arg Leu Asp Ser 
                85                  90                  95      


Pro Val Lys Asn Ser Lys His Ile Ala Pro Phe Ser Leu Pro Ser Ser 
            100                 105                 110         


Pro Pro Ser Val Gly Ser Val Cys Arg Ile Met Gly Trp Gly Arg Ile 
        115                 120                 125             


Ser Pro Thr Glu Gly Thr Tyr Pro Asp Val Pro His Cys Val Asn Ile 
    130                 135                 140                 


Asn Leu Leu Glu Tyr Glu Met Cys Arg Ala Pro Tyr Pro Glu Phe Glu 
145                 150                 155                 160 


Leu Pro Ala Thr Ser Arg Thr Leu Cys Ala Gly Ile Leu Glu Gly Gly 
                165                 170                 175     


Lys Asp Thr Cys Lys Gly Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly 
            180                 185                 190         


Gln Phe Gln Gly Ile Ala Ser Trp Gly Asp Asp Pro Cys Ala Gln Pro 
        195                 200                 205             


His Lys Pro Ala Ala Tyr Thr Lys Val Phe Asp His Leu Asp Trp Ile 
    210                 215                 220                 


<210>  5
<211>  222
<212>  PRT
<213>  Gloydius halys江浙蝮蛇

<400>  5

Val Ile Gly Gly Asp Glu Cys Asn Ile Asn Glu His Arg Phe Leu Val 
1               5                   10                  15      


Ala Leu Tyr Thr Ser Arg Thr Leu Phe Cys Gly Gly Thr Leu Ile Asn 
            20                  25                  30          


Gln Glu Trp Val Leu Thr Ala Ala His Cys Asn Met Glu Asp Ile Gln 
        35                  40                  45              


Ile Lys Leu Gly Met His Ser Lys Lys Val Pro Asn Glu Asp Glu Gln 
    50                  55                  60                  


Lys Arg Val Pro Lys Glu Lys Phe Phe Cys Leu Ser Ser Lys Lys Leu 
65                  70                  75                  80  


Tyr Leu Trp Asp Lys Asp Ile Met Leu Ile Arg Leu Asp Ser Pro Val 
                85                  90                  95      


Lys Asn Ser Ala His Ile Ala Pro Leu Ser Leu Pro Ser Ser Pro Pro 
            100                 105                 110         


Ser Val Gly Ser Val Cys Arg Thr Met Gly Trp Gly Arg Ile Ser Ser 
        115                 120                 125             


Thr Lys Glu Thr Tyr Pro Asp Val Pro His Cys Val Asn Ile Asn Leu 
    130                 135                 140                 


Leu Glu Tyr Glu Met Cys Arg Ala Pro Tyr Pro Glu Phe Glu Leu Pro 
145                 150                 155                 160 


Ala Thr Ser Arg Thr Leu Cys Ala Gly Ile Leu Glu Gly Gly Lys Asp 
                165                 170                 175     


Thr Cys Val Gly Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly Gln Phe 
            180                 185                 190         


Gln Gly Ile Ala Ser Trp Gly Asp His Pro Cys Ala Gln Pro His Lys 
        195                 200                 205             


Pro Ala Ala Tyr Thr Lys Val Phe Asp His Leu Asp Trp Ile 
    210                 215                 220         


<210>  6
<211>  220
<212>  PRT
<213>  Crotalus adamanteus响尾蛇

<400>  6

Val Ile Gly Gly Asp Glu Cys Asn Ile Asn Glu His Arg Phe Leu Val 
1               5                   10                  15      


Ala Leu Tyr Asp Tyr Trp Ser Gln Ser Phe Leu Cys Gly Gly Thr Leu 
            20                  25                  30          


Ile Asn Glu Glu Trp Val Leu Thr Ala Lys His Cys Asp Arg Thr His 
        35                  40                  45              


Ile Leu Ile Tyr Val Gly Val His Asp Arg Ser Val Gln Phe Asp Lys 
    50                  55                  60                  


Glu Gln Arg Arg Phe Pro Lys Glu Lys Tyr Phe Phe Asp Cys Ser Asn 
65                  70                  75                  80  


Asn Phe Thr Lys Trp Asp Lys Asp Ile Met Leu Ile Arg Leu Asn Lys 
                85                  90                  95      


Pro Val Ser Tyr Ser Glu His Ile Ala Pro Leu Ser Leu Pro Ser Ser 
            100                 105                 110         


Pro Pro Ile Val Gly Ser Val Cys Arg Ala Met Gly Trp Gly Gln Thr 
        115                 120                 125             


Thr Ser Pro Gln Glu Thr Leu Pro Asp Val Pro His Cys Ala Asn Ile 
    130                 135                 140                 


Asn Leu Leu Asp Tyr Glu Val Cys Arg Thr Ala His Pro Gln Phe Arg 
145                 150                 155                 160 


Leu Pro Ala Thr Ser Arg Thr Leu Cys Ala Gly Val Leu Glu Gly Gly 
                165                 170                 175     


Ile Asp Thr Cys Asn Arg Asp Ser Gly Gly Pro Leu Ile Cys Asn Gly 
            180                 185                 190         


Gln Phe Gln Gly Ile Val Phe Trp Gly Pro Asp Pro Cys Ala Gln Pro 
        195                 200                 205             


Asp Lys Pro Gly Leu Tyr Thr Lys Leu Asp Trp Ile 
    210                 215                 220 


