﻿                         序列表

<120>  Sip1Aa可溶性杀虫蛋白突变体

<160>  6

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  364
<212>  PRT
<213>  苏云金芽孢杆菌(Bacillus thuringiensis)

<400>  1
Met Lys Tyr Lys Phe Ser Lys Val Val Lys Cys Thr Leu Pro Ala Leu 
1               5                   10                  15      
Met Ile Thr Thr Phe Val Thr Pro Ser Met Ala Val Phe Ala Ala Glu 
            20                  25                  30          
Thr Lys Ser Pro Asn Leu Asn Ala Ser Gln Gln Ala Ile Thr Pro Tyr 
        35                  40                  45              
Ala Glu Ser Tyr Ile Asp Thr Val Gln Asp Arg Met Lys Gln Arg Asp 
    50                  55                  60                  
Arg Glu Ser Lys Leu Thr Gly Lys Pro Ile Asn Met Gln Glu Gln Ile 
65                  70                  75                  80  
Ile Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Phe Lys Asp Gln Asn 
                85                  90                  95      
Asn Asn His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asp Asn Leu 
            100                 105                 110         
Ala Ser Ser Lys Gly Tyr Asp Ser Ile Ala Glu Gln Met Gly Leu Lys 
        115                 120                 125             
Ile Glu Ala Leu Asn Asp Met Asp Val Thr Asn Ile Asp Tyr Thr Ser 
    130                 135                 140                 
Lys Thr Gly Asp Thr Ile Tyr Asn Gly Ile Ser Glu Leu Thr Asn Tyr 
145                 150                 155                 160 
Thr Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Arg Asp Tyr 
                165                 170                 175     
Thr Lys Ser Glu Ser Thr Ser Val Thr Asn Gly Leu Gln Leu Gly Phe 
            180                 185                 190         
Lys Val Ala Ala Lys Gly Val Val Ala Leu Ala Gly Ala Asp Phe Glu 
        195                 200                 205             
Thr Ser Val Thr Tyr Asn Leu Ser Ser Thr Thr Thr Glu Thr Asn Thr 
    210                 215                 220                 
Ile Ser Asp Lys Phe Thr Val Pro Ser Gln Glu Val Thr Leu Ser Pro 
225                 230                 235                 240 
Gly His Lys Ala Val Val Lys His Asp Leu Arg Lys Met Val Tyr Phe 
                245                 250                 255     
Gly Thr Gln Asp Leu Lys Gly Asp Leu Lys Val Ser Phe Asn Asp Lys 
            260                 265                 270         
Glu Ile Val Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Ser Ile Asp Leu 
        275                 280                 285             
Ser Asp Ile Arg Lys Thr Met Ile Glu Ile Asp Lys Trp Asn His Val 
    290                 295                 300                 
Asn Thr Ile Asp Phe Tyr Gln Leu Val Gly Val Lys Asn His Ile Lys 
305                 310                 315                 320 
Asn Gly Asp Thr Leu Tyr Ile Asp Thr Pro Ala Glu Phe Thr Phe Asn 
                325                 330                 335     
Gly Ala Asn Pro Tyr Tyr Arg Ala Thr Phe Thr Glu Tyr Asp Glu Asn 
            340                 345                 350         
Gly Asn Pro Val Gln Thr Lys Ile Leu Ser Gly Asn 
        355                 360                 

<210>  2
<211>  1095
<212>  DNA
<213>  苏云金芽孢杆菌(Bacillus thuringiensis)

<400>  2
atgaaataca agttttcaaa agtcgttaag tgtactttac cagctttaat gattactaca        60
ttcgttactc caagtatggc agtttttgcc gcagaaacca agtcgccaaa tctaaatgca       120
tctcaacaag caataactcc atatgctgaa tcttatattg atacggttca agatagaatg       180
aaacaaagag atagggaatc aaaactaact ggtaagccaa taaatatgca agaacaaata       240
atagatggat ggtttttagc tagattctgg atatttaaag atcaaaataa caatcatcaa       300
acaaatagat ttatatcctg gtttaaagat aatcttgcta gttcgaaggg gtatgacagt       360
atagcagaac aaatgggctt aaaaatagaa gcattaaatg atatggatgt aacaaatatt       420
gattatacat ctaaaacagg tgataccata tataatggaa tttctgaact aacaaattat       480
acaggaacaa cccaaaaaat gaaaaccgat agttttcaaa gagattatac aaaatctgaa       540
tccacttcag taacaaatgg gttacaatta ggatttaaag ttgctgctaa gggagtagtt       600
gcattagcag gtgcagattt tgaaacaagt gttacctata atttatcatc tactacaact       660
gaaacaaata caatatcgga taagtttact gttccatctc aagaagttac attatcccca       720
ggtcataaag cagtagtgaa acatgatttg agaaaaatgg tgtattttgg gactcaagat       780
ttaaagggtg atttaaaagt aagttttaat gataaagaga ttgtacaaaa atttatttat       840
ccaaattata gatcaattga tttatctgat attcgtaaaa caatgattga aattgataaa       900
tggaatcatg taaataccat tgacttttat caattagttg gagttaaaaa tcatataaaa       960
aatggtgata ctttatatat agataccccg gccgaattta catttaatgg agctaatcca      1020
tattatagag caacatttac agaatacgac gagaacggaa atcctgttca aacaaagatt      1080
ttaagtggaa attaa                                                       1095

<210>  3
<211>  364
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  3
Met Lys Tyr Lys Phe Ser Lys Val Val Lys Cys Thr Leu Pro Ala Leu 
1               5                   10                  15      
Met Ile Thr Thr Phe Val Thr Pro Ser Met Ala Val Phe Ala Ala Glu 
            20                  25                  30          
Thr Lys Ser Pro Asn Leu Asn Ala Ser Gln Gln Ala Ile Thr Pro Tyr 
        35                  40                  45              
Ala Glu Ser Tyr Ile Asp Thr Val Gln Asp Arg Met Lys Gln Arg Asp 
    50                  55                  60                  
Arg Glu Ser Lys Leu Thr Gly Lys Pro Ile Asn Met Gln Glu Gln Ile 
65                  70                  75                  80  
Ile Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Phe Lys Asp Gln Asn 
                85                  90                  95      
Asn Asn His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asp Asn Leu 
            100                 105                 110         
Ala Ser Ser Lys Gly Tyr Asp Ser Ile Ala Glu Gln Met Gly Leu Lys 
        115                 120                 125             
Ile Glu Ala Leu Asn Asp Met Asp Val Thr Asn Ile Asp Tyr Thr Ser 
    130                 135                 140                 
Lys Thr Gly Asp Thr Ile Tyr Asn Cys Ile Ser Glu Leu Thr Asn Tyr 
145                 150                 155                 160 
Thr Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Arg Asp Tyr 
                165                 170                 175     
Thr Lys Ser Glu Ser Thr Ser Val Thr Asn Gly Leu Gln Leu Gly Phe 
            180                 185                 190         
Lys Val Ala Ala Lys Gly Val Val Ala Leu Ala Gly Ala Asp Phe Glu 
        195                 200                 205             
Thr Ser Val Thr Tyr Asn Leu Ser Ser Thr Thr Thr Glu Thr Asn Thr 
    210                 215                 220                 
Ile Ser Asp Lys Phe Thr Val Pro Ser Gln Glu Val Thr Leu Ser Pro 
225                 230                 235                 240 
Gly His Lys Ala Val Val Lys Cys Asp Leu Arg Lys Met Val Tyr Phe 
                245                 250                 255     
Gly Thr Gln Asp Leu Lys Gly Asp Leu Lys Val Ser Phe Asn Asp Lys 
            260                 265                 270         
Glu Ile Val Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Ser Ile Asp Leu 
        275                 280                 285             
Ser Asp Ile Arg Lys Thr Met Ile Glu Ile Asp Lys Trp Asn His Val 
    290                 295                 300                 
Asn Thr Ile Asp Phe Tyr Gln Leu Val Gly Val Lys Asn His Ile Lys 
305                 310                 315                 320 
Asn Gly Asp Thr Leu Tyr Ile Asp Thr Pro Ala Glu Phe Thr Phe Asn 
                325                 330                 335     
Gly Ala Asn Pro Tyr Tyr Arg Ala Thr Phe Thr Glu Tyr Asp Glu Asn 
            340                 345                 350         
Gly Asn Pro Val Gln Thr Lys Ile Leu Ser Gly Asn 
        355                 360                 

<210>  4
<211>  1095
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
atgaaataca agttttcaaa agtcgttaag tgtactttac cagctttaat gattactaca        60
ttcgttactc caagtatggc agtttttgcc gcagaaacca agtcgccaaa tctaaatgca       120
tctcaacaag caataactcc atatgctgaa tcttatattg atacggttca agatagaatg       180
aaacaaagag atagggaatc aaaactaact ggtaagccaa taaatatgca agaacaaata       240
atagatggat ggtttttagc tagattctgg atatttaaag atcaaaataa caatcatcaa       300
acaaatagat ttatatcctg gtttaaagat aatcttgcta gttcgaaggg gtatgacagt       360
atagcagaac aaatgggctt aaaaatagaa gcattaaatg atatggatgt aacaaatatt       420
gattatacat ctaaaacagg tgataccata tataattgta tttctgaact aacaaattat       480
acaggaacaa cccaaaaaat gaaaaccgat agttttcaaa gagattatac aaaatctgaa       540
tccacttcag taacaaatgg gttacaatta ggatttaaag ttgctgctaa gggagtagtt       600
gcattagcag gtgcagattt tgaaacaagt gttacctata atttatcatc tactacaact       660
gaaacaaata caatatcgga taagtttact gttccatctc aagaagttac attatcccca       720
ggtcataaag cagtagtgaa atgtgatttg agaaaaatgg tgtattttgg gactcaagat       780
ttaaagggtg atttaaaagt aagttttaat gataaagaga ttgtacaaaa atttatttat       840
ccaaattata gatcaattga tttatctgat attcgtaaaa caatgattga aattgataaa       900
tggaatcatg taaataccat tgacttttat caattagttg gagttaaaaa tcatataaaa       960
aatggtgata ctttatatat agataccccg gccgaattta catttaatgg agctaatcca      1020
tattatagag caacatttac agaatacgac gagaacggaa atcctgttca aacaaagatt      1080
ttaagtggaa attaa                                                       1095

<210>  5
<211>  364
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  5
Met Lys Tyr Lys Phe Ser Lys Val Val Lys Cys Thr Leu Pro Ala Leu 
1               5                   10                  15      
Met Ile Thr Thr Phe Val Thr Pro Ser Met Ala Val Phe Ala Ala Glu 
            20                  25                  30          
Thr Lys Ser Pro Asn Leu Asn Ala Ser Gln Gln Ala Ile Thr Pro Tyr 
        35                  40                  45              
Ala Glu Ser Tyr Ile Asp Thr Val Gln Asp Arg Met Lys Gln Arg Asp 
    50                  55                  60                  
Arg Glu Ser Lys Leu Thr Gly Lys Pro Ile Asn Met Gln Glu Gln Ile 
65                  70                  75                  80  
Ile Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Phe Lys Asp Gln Asn 
                85                  90                  95      
Asn Asn His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asp Asn Leu 
            100                 105                 110         
Ala Ser Ser Lys Gly Tyr Asp Ser Ile Ala Glu Gln Met Gly Leu Lys 
        115                 120                 125             
Ile Glu Ala Leu Asn Asp Met Asp Val Thr Asn Ile Asp Tyr Thr Ser 
    130                 135                 140                 
Lys Thr Gly Asp Thr Ile Tyr Asn Gly Ile Ser Glu Leu Cys Asn Tyr 
145                 150                 155                 160 
Thr Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Arg Asp Tyr 
                165                 170                 175     
Thr Lys Ser Glu Ser Thr Ser Val Thr Asn Gly Leu Gln Leu Gly Phe 
            180                 185                 190         
Lys Val Ala Ala Lys Gly Val Val Ala Leu Ala Gly Ala Asp Phe Glu 
        195                 200                 205             
Thr Ser Val Thr Tyr Asn Leu Ser Ser Thr Thr Thr Glu Thr Asn Thr 
    210                 215                 220                 
Ile Ser Asp Lys Phe Thr Val Pro Ser Gln Glu Val Thr Leu Ser Pro 
225                 230                 235                 240 
Gly His Cys Ala Val Val Lys His Asp Leu Arg Lys Met Val Tyr Phe 
                245                 250                 255     
Gly Thr Gln Asp Leu Lys Gly Asp Leu Lys Val Ser Phe Asn Asp Lys 
            260                 265                 270         
Glu Ile Val Gln Lys Phe Ile Tyr Pro Asn Tyr Arg Ser Ile Asp Leu 
        275                 280                 285             
Ser Asp Ile Arg Lys Thr Met Ile Glu Ile Asp Lys Trp Asn His Val 
    290                 295                 300                 
Asn Thr Ile Asp Phe Tyr Gln Leu Val Gly Val Lys Asn His Ile Lys 
305                 310                 315                 320 
Asn Gly Asp Thr Leu Tyr Ile Asp Thr Pro Ala Glu Phe Thr Phe Asn 
                325                 330                 335     
Gly Ala Asn Pro Tyr Tyr Arg Ala Thr Phe Thr Glu Tyr Asp Glu Asn 
            340                 345                 350         
Gly Asn Pro Val Gln Thr Lys Ile Leu Ser Gly Asn 
        355                 360                 

<210>  6
<211>  1095
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
atgaaataca agttttcaaa agtcgttaag tgtactttac cagctttaat gattactaca        60
ttcgttactc caagtatggc agtttttgcc gcagaaacca agtcgccaaa tctaaatgca       120
tctcaacaag caataactcc atatgctgaa tcttatattg atacggttca agatagaatg       180
aaacaaagag atagggaatc aaaactaact ggtaagccaa taaatatgca agaacaaata       240
atagatggat ggtttttagc tagattctgg atatttaaag atcaaaataa caatcatcaa       300
acaaatagat ttatatcctg gtttaaagat aatcttgcta gttcgaaggg gtatgacagt       360
atagcagaac aaatgggctt aaaaatagaa gcattaaatg atatggatgt aacaaatatt       420
gattatacat ctaaaacagg tgataccata tataatggaa tttctgaact atgtaattat       480
acaggaacaa cccaaaaaat gaaaaccgat agttttcaaa gagattatac aaaatctgaa       540
tccacttcag taacaaatgg gttacaatta ggatttaaag ttgctgctaa gggagtagtt       600
gcattagcag gtgcagattt tgaaacaagt gttacctata atttatcatc tactacaact       660
gaaacaaata caatatcgga taagtttact gttccatctc aagaagttac attatcccca       720
ggtcattgtg cagtagtgaa acatgatttg agaaaaatgg tgtattttgg gactcaagat       780
ttaaagggtg atttaaaagt aagttttaat gataaagaga ttgtacaaaa atttatttat       840
ccaaattata gatcaattga tttatctgat attcgtaaaa caatgattga aattgataaa       900
tggaatcatg taaataccat tgacttttat caattagttg gagttaaaaa tcatataaaa       960
aatggtgata ctttatatat agataccccg gccgaattta catttaatgg agctaatcca      1020
tattatagag caacatttac agaatacgac gagaacggaa atcctgttca aacaaagatt      1080
ttaagtggaa attaa                                                       1095

