                         SEQUENCE LISTING

<110>  Merck Sharp & dohme Corp.
       Bailly, Marc A
       Chakravarthy, Kalyan
       Fayad, Ghassan
       Fayadat-Dilman, Laurence
       Kofman, Esther
       Handa, Mas
       O'Neil, Jennifer
       Palte, Rachel
       Scapin, Giovanna
       Taremi, Shahriar Shane
 
<120>  ARGINASE 1 BINDERS FOR INHIBITING ARGINASE 1 ACTIVITY

<130>  25134

<150>  63/119,265
<151>  2020-11-30

<160>  81    

<170>  PatentIn version 3.5

<210>  1
<211>  322
<212>  PRT
<213>  Homo Sapiens

<400>  1

Met Ser Ala Lys Ser Arg Thr Ile Gly Ile Ile Gly Ala Pro Phe Ser 
1               5                   10                  15      


Lys Gly Gln Pro Arg Gly Gly Val Glu Glu Gly Pro Thr Val Leu Arg 
            20                  25                  30          


Lys Ala Gly Leu Leu Glu Lys Leu Lys Glu Gln Glu Cys Asp Val Lys 
        35                  40                  45              


Asp Tyr Gly Asp Leu Pro Phe Ala Asp Ile Pro Asn Asp Ser Pro Phe 
    50                  55                  60                  


Gln Ile Val Lys Asn Pro Arg Ser Val Gly Lys Ala Ser Glu Gln Leu 
65                  70                  75                  80  


Ala Gly Lys Val Ala Glu Val Lys Lys Asn Gly Arg Ile Ser Leu Val 
                85                  90                  95      


Leu Gly Gly Asp His Ser Leu Ala Ile Gly Ser Ile Ser Gly His Ala 
            100                 105                 110         


Arg Val His Pro Asp Leu Gly Val Ile Trp Val Asp Ala His Thr Asp 
        115                 120                 125             


Ile Asn Thr Pro Leu Thr Thr Thr Ser Gly Asn Leu His Gly Gln Pro 
    130                 135                 140                 


Val Ser Phe Leu Leu Lys Glu Leu Lys Gly Lys Ile Pro Asp Val Pro 
145                 150                 155                 160 


Gly Phe Ser Trp Val Thr Pro Cys Ile Ser Ala Lys Asp Ile Val Tyr 
                165                 170                 175     


Ile Gly Leu Arg Asp Val Asp Pro Gly Glu His Tyr Ile Leu Lys Thr 
            180                 185                 190         


Leu Gly Ile Lys Tyr Phe Ser Met Thr Glu Val Asp Arg Leu Gly Ile 
        195                 200                 205             


Gly Lys Val Met Glu Glu Thr Leu Ser Tyr Leu Leu Gly Arg Lys Lys 
    210                 215                 220                 


Arg Pro Ile His Leu Ser Phe Asp Val Asp Gly Leu Asp Pro Ser Phe 
225                 230                 235                 240 


Thr Pro Ala Thr Gly Thr Pro Val Val Gly Gly Leu Thr Tyr Arg Glu 
                245                 250                 255     


Gly Leu Tyr Ile Thr Glu Glu Ile Tyr Lys Thr Gly Leu Leu Ser Gly 
            260                 265                 270         


Leu Asp Ile Met Glu Val Asn Pro Ser Leu Gly Lys Thr Pro Glu Glu 
        275                 280                 285             


Val Thr Arg Thr Val Asn Thr Ala Val Ala Ile Thr Leu Ala Cys Phe 
    290                 295                 300                 


Gly Leu Ala Arg Glu Gly Asn His Lys Pro Ile Asp Tyr Leu Asn Pro 
305                 310                 315                 320 


Pro Lys 
        


<210>  2
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Arg Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          


Gly Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Asn Trp Asn Gly Gly Ser Thr Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr His Cys 
                85                  90                  95      


Ala Arg Asp Arg Arg Arg Gly Ser Tyr Gly Ser Asp Ala Phe Asp Ile 
            100                 105                 110         


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser 
        115                 120             


<210>  3
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Gln Leu Leu Ile 
        35                  40                  45              


Ser Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln Lys Tyr Asn Ser Ala Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 Kabat

<400>  4

Asp Tyr Gly Met Thr 
1               5   


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 Kabat/Kabat+Chothia

<400>  5

Gly Ile Asn Trp Asn Gly Gly Ser Thr Gly Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 Kabat/Chothia/Kabat+Chothia/AbM

<400>  6

Asp Arg Arg Arg Gly Ser Tyr Gly Ser Asp Ala Phe Asp Ile 
1               5                   10                  


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 Chothia

<400>  7

Gly Phe Thr Phe Asp Asp Tyr 
1               5           


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 Chothia

<400>  8

Asn Trp Asn Gly Gly Ser 
1               5       


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 Kabat+Chothia/AbM

<400>  9

Gly Phe Thr Phe Asp Asp Tyr Gly Met Thr 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 AbM

<400>  10

Gly Ile Asn Trp Asn Gly Gly Ser Thr Gly 
1               5                   10  


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 IMGT

<400>  11

Gly Phe Thr Phe Asp Asp Tyr Gly 
1               5               


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 IMGT

<400>  12

Arg Arg Gly Ser Tyr Gly Ser Asp Ala Phe Asp Ile 
1               5                   10          


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 IMGT

<400>  13

Ala Arg Asp Arg Arg Arg Gly Ser Tyr Gly Ser Asp Ala Phe Asp Ile 
1               5                   10                  15      


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 Contact

<400>  14

Asp Asp Tyr Gly Met Thr 
1               5       


<210>  15
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 Contact

<400>  15

Trp Val Ser Gly Ile Asn Trp Asn Gly Gly Ser Thr Gly 
1               5                   10              


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 Contact

<400>  16

Ala Arg Asp Arg Arg Arg Gly Ser Tyr Gly Ser Asp Ala Phe Asp 
1               5                   10                  15  


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 Kabat/Chothia/Kabat+Chothia/AbM

<400>  17

Arg Ala Ser Gln Gly Ile Ser Asn Tyr Leu Ala 
1               5                   10      


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 Kabat/Chothia/Kabat+Chothia/AbM

<400>  18

Ala Ala Ser Thr Leu Gln Ser 
1               5           


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR3 Kabat/Chothia/Kabat+Chothia/AbM/IMGT

<400>  19

Gln Lys Tyr Asn Ser Ala Pro Arg Thr 
1               5                   


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 IMGT

<400>  20

Gln Gly Ile Ser Asn Tyr 
1               5       


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 Contact

<400>  21

Ser Asn Tyr Leu Ala Trp Tyr 
1               5           


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 Contact

<400>  22

Leu Leu Ile Ser Ala Ala Ser Thr Leu Gln 
1               5                   10  


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR3 Contact

<400>  23

Gln Lys Tyr Asn Ser Ala Pro Arg 
1               5               


<210>  24
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC constant domain

<400>  24

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  25
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (L234A L235A D265S)

<400>  25

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Ser Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  26
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (L234A L235A P329G)

<400>  26

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Ser Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  27
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (L235E)

<400>  27

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  28
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (D265A)

<400>  28

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Ala Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  29
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (D265A N297G)

<400>  29

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Ala Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Gly Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  30
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (E233A/L235A)

<400>  30

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Ala Leu Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  31
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (N297X


<220>
<221>  MISC_FEATURE
<222>  (180)..(180)
<223>  Xaa is any amino acid other than Asn

<400>  31

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Xaa Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  32
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain

<400>  32

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  33
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (D265S)

<400>  33

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Ser 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  34
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (P329G)

<400>  34

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Gly 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  35
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (D265A)

<400>  35

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Ala 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  36
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (D265A N297G)

<400>  36

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Ala 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gly 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  37
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (N297X)


<220>
<221>  MISC_FEATURE
<222>  (176)..(176)
<223>  Xaa is any amino acid other than Asn

<400>  37

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Xaa 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ser Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  38
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 HC Constant domain (V234A G237A P238S H268A V309L 
       A330S P331S X378S/A)


<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa can be any naturally occurring amino acid

<400>  38

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Ala Ala Ala Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser Ala Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ser Ser Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Xaa Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  39
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 HC Constant domain (S228P)

<400>  39

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  40
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 HC Constant domain (S228P P329G)

<400>  40

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Gly Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  41
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 HC Constant domain (S228P D265A)

<400>  41

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Ala Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  42
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 HC Constant domain (S228P D265A N297G)

<400>  42

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Ala Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Gly Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  43
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 HC Constant domain (S228P N297X)


<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is any amino acid other than Asn

<400>  43

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Xaa Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  44
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human LC Kappa Constant domain

<400>  44

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  45
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (N297A/D356E/L358M)

<400>  45

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  46
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 HC Constant domain (L234F/L235E/P331S/D356E/L358M)

<400>  46

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Leu Thr Cys Leu Val Lys 
225                 230                 235                 240 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                245                 250                 255     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            260                 265                 270         


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
        275                 280                 285             


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    290                 295                 300                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
305                 310                 315         


<210>  47
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human LC lambda Constant domain

<400>  47

Gly Gln Pro Lys Ala Asn Pro Thr Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      


Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          


Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Gly Ser Pro 
        35                  40                  45              


Val Lys Ala Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys 
65                  70                  75                  80  


Ser His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      


Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105     


<210>  48
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse HC IgG1 (D265A) constant domain

<400>  48

Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala 
1               5                   10                  15      


Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu 
    50                  55                  60                  


Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro 
            100                 105                 110         


Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu 
        115                 120                 125             


Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Ala Ile Ser 
    130                 135                 140                 


Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro 
        195                 200                 205             


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln 
    210                 215                 220                 


Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val 
225                 230                 235                 240 


Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val 
                245                 250                 255     


Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln 
            260                 265                 270         


Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn 
        275                 280                 285             


Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val 
    290                 295                 300                 


Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser His 
305                 310                 315                 320 


Ser Pro Gly Lys 
                


<210>  49
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse HC IgG2 constant domain

<400>  49

Ala Lys Thr Thr Ala Pro Ser Val Tyr Pro Leu Ala Pro Val Cys Gly 
1               5                   10                  15      


Asp Thr Thr Gly Ser Ser Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Leu Thr Trp Asn Ser Gly Ser Leu Ser Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu 
    50                  55                  60                  


Ser Ser Ser Val Thr Val Thr Ser Ser Thr Trp Pro Ser Gln Ser Ile 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Ile Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys 
            100                 105                 110         


Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro 
        115                 120                 125             


Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp 
145                 150                 155                 160 


Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg 
                165                 170                 175     


Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln 
            180                 185                 190         


His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn 
        195                 200                 205             


Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly 
    210                 215                 220                 


Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu 
225                 230                 235                 240 


Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met 
                245                 250                 255     


Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu 
            260                 265                 270         


Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe 
        275                 280                 285             


Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn 
    290                 295                 300                 


Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His His Thr 
305                 310                 315                 320 


Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
                325                 330 


<210>  50
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse LC kappa constant domain

<400>  50

Arg Thr Val Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
1               5                   10                  15      


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
        35                  40                  45              


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
65                  70                  75                  80  


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
                85                  90                  95      


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
            100                 105         


<210>  51
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VH1

<400>  51
caggtgcagc tggtgcagag cggcgccgag gtgaagaagc ccggcgccag cgtgaaggtg       60

agctgcaagg ccagcggcta caccttcacc aactacggca tcagctgggt gagacaggcc      120

cccggccagg gcctggagtg gatgggctgg atcagcgcct acaacggcaa caccaactac      180

gcccagaagc tgcagggcag agtgaccatg accaccgaca ccagcaccag caccgcctac      240

atggagctga gaagcctgag aagcgacgac accgccgtgt actactgcgc cagagagggc      300

gcctacggct acagaagccc ctaccacaac tggttcgacc cctggggcca gggcaccctg      360

gtgaccgtga gcagc                                                       375


<210>  52
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VH2

<400>  52
gaggtgcagc tggtgcagag cggcgccgag gtgaagaagc ccggcgccag cgtgaaggtg       60

agctgcaagg ccagcggcta caccttcttc aagtacggca tcagctgggt gagacaggcc      120

cccggccagg gcctggagtg gatgggcagc atcagcccct acaccggcga gacccactac      180

gcccagaagc tgcagggcag agtgaccatg accaccgaca ccagcaccag caccgcctac      240

atggagctga gaagcctgag aagcgacgac accgccgtgt actactgcgc cagagagggc      300

gcctacggct acagaagccc ctaccagaac tggttcgacc cctggggcca gggcaccctg      360

gtgaccgtga gcagc                                                       375


<210>  53
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VL

<400>  53
gagatcgtga tgacccagag ccccgccacc ctgagcctga gccccggcga gagagccacc       60

ctgagctgca gagccagcca gagcgtgagc agctacctgg cctggtacca gcagaagccc      120

ggccaggccc ccagactgct gatctacgac gccagcaaca gagccaccgg catccccgcc      180

agattcagcg gcagcggcag cggcaccgac ttcaccctga ccatcagcag cctggagccc      240

gaggacttcg ccgtgtacta ctgccagcag cacagcctgc tgcccagaac cttcggcggc      300

ggcaccaagg tggagatcaa g                                                321


<210>  54
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC constant domain

<400>  54
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  55
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain (L234A
       L235A D265S)

<400>  55
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgaggc cgccggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt gagcgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  56
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain (L234A
       L235A P329G)

<400>  56
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgaggc cgccggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt gagcgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgggcgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  57
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (L235E)

<400>  57
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct ggagggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  58
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (D265A)

<400>  58
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggccgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  59
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain (D265A
       N297G)

<400>  59
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggccgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacggc      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  60
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (E233A/L235A)

<400>  60
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgccct ggccggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  61
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (N297X, wherein X is any amino acid other than N)


<220>
<221>  misc_feature
<222>  (538)..(540)
<223>  Codon wherein each N is any nucleotide with the proviso that the 
       codon does not encode Asn or form a translation stop codon

<400>  61
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacnnn      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagacgag      720

ctgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  62
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain

<400>  62
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg acgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcaacag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  63
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain 
       (D265S)

<400>  63
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtga gcgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcaacag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  64
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain 
       (P329G)

<400>  64
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg acgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcaacag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gggcgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  65
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain 
       (D265A)

<400>  65
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg ccgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcaacag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  66
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain (D265A
       N297G)

<400>  66
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg ccgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcggcag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  67
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain 
       (N297X, wherein X is any amino acid other than N)


<220>
<221>  misc_feature
<222>  (526)..(528)
<223>  Codon wherein each N is any nucleotide with the proviso that the 
       codon does not encode Asn or form a translation stop codon

<400>  67
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg tggccggccc cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg acgtgagcca cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcnnnag caccttcaga      540

gtggtgagcg tgctgaccgt ggtgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccgccccc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcag cgtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  68
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG2 HC Constant domain (V234A
       G237A P238S H268A V309L A330S P331S X378S/A)


<220>
<221>  misc_feature
<222>  (769)..(771)
<223>  n is a, c, g, or t

<400>  68
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcaacttcgg cacccagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagac cgtggagaga      300

aagtgctgcg tggagtgccc cccctgcccc gccccccccg ccgccgccag cagcgtgttc      360

ctgttccccc ccaagcccaa ggacaccctg atgatcagca gaacccccga ggtgacctgc      420

gtggtggtgg acgtgagcgc cgaggacccc gaggtgcagt tcaactggta cgtggacggc      480

gtggaggtgc acaacgccaa gaccaagccc agagaggagc agttcaacag caccttcaga      540

gtggtgagcg tgctgaccgt gctgcaccag gactggctga acggcaagga gtacaagtgc      600

aaggtgagca acaagggcct gcccagcagc atcgagaaga ccatcagcaa gaccaagggc      660

cagcccagag agccccaggt gtacaccctg ccccccagca gagaggagat gaccaagaac      720

caggtgagcc tgacctgcct ggtgaagggc ttctacccca gcgacatcnn ngtggagtgg      780

gagagcaacg gccagcccga gaacaactac aagaccaccc cccccatgct ggacagcgac      840

ggcagcttct tcctgtacag caagctgacc gtggacaaga gcagatggca gcagggcaac      900

gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagagcctg      960

agcctgagcc ccggcaag                                                    978


<210>  69
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG4 HC Constant domain 
       (S228P)

<400>  69
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggacgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcaa cagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgcccagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gccaggagga gatgaccaag      720

aaccaggtga gcctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      840

gacggcagct tcttcctgta cagcagactg accgtggaca agagcagatg gcaggagggc      900

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      960

ctgagcctga gcctgggcaa g                                                981


<210>  70
<211>  960
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG4 HC Constant domain (S228P
       P329G)

<400>  70
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggacgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcaa cagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgggcagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gccaggagga gatgaccaag      720

aaccaggtga gcctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      840

gacggcagct tcttcctgta cagcagactg accgtggaca agagcagatg gcaggagggc      900

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      960


<210>  71
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG4 HC Constant domain (S228P
       D265A)

<400>  71
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggccgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcaa cagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgcccagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gccaggagga gatgaccaag      720

aaccaggtga gcctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      840

gacggcagct tcttcctgta cagcagactg accgtggaca agagcagatg gcaggagggc      900

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      960

ctgagcctga gcctgggcaa g                                                981


<210>  72
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG4 HC Constant domain (S228P
       D265A N297G)

<400>  72
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggccgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcgg cagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgcccagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gccaggagga gatgaccaag      720

aaccaggtga gcctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      840

gacggcagct tcttcctgta cagcagactg accgtggaca agagcagatg gcaggagggc      900

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      960

ctgagcctga gcctgggcaa g                                                981


<210>  73
<211>  981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG4 HC Constant domain (S228P
       N297X, wherein X is any amino acid other than N)


<220>
<221>  misc_feature
<222>  (529)..(531)
<223>  Codon wherein each N is any nucleotide with the proviso that the 
       codon does not encode Asn or form a transaltion stop codon

<400>  73
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggacgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcnn nagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgcccagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gccaggagga gatgaccaag      720

aaccaggtga gcctgacctg cctggtgaag ggcttctacc ccagcgacat cgccgtggag      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc      840

gacggcagct tcttcctgta cagcagactg accgtggaca agagcagatg gcaggagggc      900

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc      960

ctgagcctga gcctgggcaa g                                                981


<210>  74
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human LC Kappa  Constant domain

<400>  74
agaaccgtgg ccgcccccag cgtgttcatc ttccccccca gcgacgagca gctgaagagc       60

ggcaccgcca gcgtggtgtg cctgctgaac aacttctacc ccagagaggc caaggtgcag      120

tggaaggtgg acaacgccct gcagagcggc aacagccagg agagcgtgac cgagcaggac      180

agcaaggaca gcacctacag cctgagcagc accctgaccc tgagcaaggc cgactacgag      240

aagcacaagg tgtacgcctg cgaggtgacc caccagggcc tgagcagccc cgtgaccaag      300

agcttcaaca gaggcgagtg c                                                321


<210>  75
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (N297A/D356E/L358M)

<400>  75
gccagcacca agggccccag cgtgttcccc ctggccccca gcagcaagag caccagcggc       60

ggcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgca acgtgaacca caagcccagc aacaccaagg tggacaagaa ggtggagccc      300

aagagctgcg acaagaccca cacctgcccc ccctgccccg cccccgagct gctgggcggc      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacgcc      540

agcacctaca gagtggtgag cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gcaaggtgag caacaaggcc ctgcccgccc ccatcgagaa gaccatcagc      660

aaggccaagg gccagcccag agagccccag gtgtacaccc tgccccccag cagagaggag      720

atgaccaaga accaggtgag cctgacctgc ctggtgaagg gcttctaccc cagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac cccccccgtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgag ccccggcaag                                       990


<210>  76
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human IgG1 HC Constant domain 
       (L234F/L235E/P331S/D356E/L358M)

<400>  76
gccagcacca agggccccag cgtgttcccc ctggccccct gcagcagaag caccagcgag       60

agcaccgccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgagc      120

tggaacagcg gcgccctgac cagcggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg caccaagacc      240

tacacctgca acgtggacca caagcccagc aacaccaagg tggacaagag agtggagagc      300

aagtacggcc ccccctgccc cccctgcccc gcccccgagt tcctgggcgg ccccagcgtg      360

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      420

tgcgtggtgg tggacgtgag ccaggaggac cccgaggtgc agttcaactg gtacgtggac      480

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagttcaa cagcacctac      540

agagtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      600

tgcaaggtga gcaacaaggg cctgcccagc agcatcgaga agaccatcag caaggccaag      660

ggccagccca gagagcccca ggtgtacacc ctgcccccca gcctgacctg cctggtgaag      720

ggcttctacc ccagcgacat cgccgtggag tgggagagca acggccagcc cgagaacaac      780

tacaagacca ccccccccgt gctggacagc gacggcagct tcttcctgta cagcagactg      840

accgtggaca agagcagatg gcaggagggc aacgtgttca gctgcagcgt gatgcacgag      900

gccctgcaca accactacac ccagaagagc ctgagcctga gcctgggcaa g               951


<210>  77
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding human LC lambda Constant domain

<400>  77
ggccagccca aggccaaccc caccgtgacc ctgttccccc ccagcagcga ggagctgcag       60

gccaacaagg ccaccctggt gtgcctgatc agcgacttct accccggcgc cgtgaccgtg      120

gcctggaagg ccgacggcag ccccgtgaag gccggcgtgg agaccaccaa gcccagcaag      180

cagagcaaca acaagtacgc cgccagcagc tacctgagcc tgacccccga gcagtggaag      240

agccacagaa gctacagctg ccaggtgacc cacgagggca gcaccgtgga gaagaccgtg      300

gcccccaccg agtgcagc                                                    318


<210>  78
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader peptide A

<400>  78

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  79
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader peptide B

<400>  79

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  80
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding leader peptide A

<400>  80
atgagcgtgc ccacccaggt gctgggcctg ctgctgctgt ggctgaccga cgccagatgc       60


<210>  81
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding leader peptide B

<400>  81
atggagtgga gctgggtgtt cctgttcttc ctgagcgtga ccaccggcgt gcacagc          57


